MD08G1121400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007104
Physical Location & Seq
Reverse (-)
11097325 .. 11102157
4833 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1121400.v1.1.491

Sequence Viewer

Length: 1344 bp
ATGGCGGCCTCCAAGCTCGCGATTCTCTCAATCTTTCTTGCCCTAATTTGCTCCCAGATTAGGGCTGACGCTTCAGTCCCGGCGGAGGAAGACGAGCCAGTGAAGCTCCTCCGATCGGATGGCCCTGATTCGTCCGCTCTGAAGATCGAGTTGGATCAGCTCAGGGCTAAGATCCACTCCCTAGAATTCGAGGTCGGTGAGAAGATTAAGGAACTAAAAGGGAAGAATGAGCTTATAGAAGAGAAGGAGAAAGTCATTCAAGAGAACTCGGATAGCATTTCTTCGTTGCAGAGTGAAATCGAGTCTCTCCAGAAAAAAGGGTCATTAGATGCTGAGCAGCTGGTTGGGAAGGCTCATGCACGTGCCGGTGAACTGGAGAAAAAGGTTAATGAACTTAAAAAGGAATTACAATCGCAACGTAAGGAGAAAGAGGGCTTGGAAGCCCGGGCAAATGAAGCTGAGAAGAAGATCGATGTTTTGCTTTTAAAAATAGGGAAGATTGAAAAGACTAATGATGAGCAGAAGACCAAAATCCGTAATACTGAGCGTGCTCTTAAAGTGGCTGAGGAGGAAATGTTAAGGGCAAAGTTTGAAGCTACTCAAAAAGCAAAAGAGTTAAATGAGGTGCATGGAGCATGGATTCCACCATGGCTTGCTGTACATTTTATGCAGTGCCAGTCCTTTCTAGAGACACACTGGAAAGTTCATGGAAAACCGGCAGTGGATATTGCAATTCAGAAGGCTCTGGAGAAAAAAGCCCAGGCTGAAAAGTGGGCTGAGCCCCATGTGGCAACATTAAAAACCAAATGGGTCCCGGCAATCAAGGAACAATGGGTGGTGGTGAAGGATACTGTTGAACCTCATGTGAAATCGTTAAGTGCAAAAACTATTGAAGTTTATGAAGTTTCAAAGACTACACTAGCTCCGCATGTTCTAAAAGCGCAGGAAGTTGTTGATCCTTATTTTCAGGAAGCAAAAAGGTTCAGCAAGCCGTATGTTGATCAGGTGGCCACTGTGGCAAAACCTCATGTTGATAAGGTTCGAGTGGTATTGAAGCCTTATACAAAGAAGGCAGTTATTGCCTACGAGAAGTTTCTCAAATTTGCAACAACATACCATCATCAGGTTCAAGGGACAGTAAAAGATTTGCTGAAGAGGCATGAACTGACAAGACCTCTTGCTACAAAGGAGTTGGAGTGGTTTGCGGCCTCGGCTTTGTTGGCCCTGCCCATCATAATTCTGTTCAGAATTTTCTCCTCACTTTTCGGCACAAAGACTAAAAAACCCGTTCGAGGTACCAACCATACACGACGTAAGGCTAAAAGGGGCCATCCAGAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

448

Amino Acids

50.68

Weight (kDa)

9.4

Isoelectric Point (pI)

29.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4686 PF15742 44 - 208 1.2e-06 Domain of unknown function (DUF4686)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
Acc65I GGTACC 1 cut(s) 1295
AccB1I GGYRCC 1 cut(s) 1295
AccBSI CCGCTC 1 cut(s) 137
AccII CGCG 1 cut(s) 20
AciI CCGC 5 cut(s) 5, 83, 135, 926, 1205
AclWI GGATC 3 cut(s) 162, 166, 950
AcoI YGGCCR 1 cut(s) 1008
AcsI RAATTY 3 cut(s) 185, 1100, 1248
AcuI CTGAAG 3 cut(s) 57, 161, 1172
AcvI CACGTG 1 cut(s) 362
AfaI GTAC 2 cut(s) 660, 1297
AfiI CCNNNNNNNGG 6 cut(s) 60, 61, 85, 115, 1123, 1292
AgsI TTSAA 8 cut(s) 260, 503, 593, 857, 893, 909, 1054, 1130
AjnI CCWGG 1 cut(s) 759
AjuI GAANNNNNNNTTGG 4 cut(s) 134, 166, 419, 451
AluBI AGCT 8 cut(s) 16, 106, 160, 232, 340, 458, 596, 923
AluI AGCT 8 cut(s) 16, 106, 160, 232, 340, 458, 596, 923
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 2 cut(s) 309, 683
AlwI GGATC 3 cut(s) 162, 166, 950
Ama87I CYCGRG 1 cut(s) 444
AoxI GGCC 6 cut(s) 6, 121, 1008, 1206, 1221, 1327
ApeKI GCWGC 1 cut(s) 337
ApoI RAATTY 3 cut(s) 185, 1100, 1248
Asp718I GGTACC 1 cut(s) 1295
AspLEI GCGC 1 cut(s) 943
AspS9I GGNCC 4 cut(s) 122, 811, 1222, 1327
AsuC2I CCSGG 4 cut(s) 80, 445, 446, 815
AsuHPI GGTGA 3 cut(s) 209, 380, 853
AvaI CYCGRG 1 cut(s) 444
AvaII GGWCC 1 cut(s) 811
BalI TGGCCA 1 cut(s) 1010
BanI GGYRCC 1 cut(s) 1295
BanII GRGCYC 1 cut(s) 783
BbrPI CACGTG 1 cut(s) 362
BbsI GAAGAC 2 cut(s) 96, 530
Bbv12I GWGCWC 1 cut(s) 553
BbvCI CCTCAGC 1 cut(s) 564
BbvI GCAGC 1 cut(s) 349
BccI CCATC 4 cut(s) 113, 1125, 1238, 1338
BceAI ACGGC 1 cut(s) 976
BciT130I CCWGG 1 cut(s) 761
BciVI GTATCC 1 cut(s) 841
BclI TGATCA 1 cut(s) 1000
BcnI CCSGG 4 cut(s) 80, 445, 446, 815
BcoDI GTCTC 2 cut(s) 309, 683
BfaI CTAG 3 cut(s) 182, 686, 920
BfuI GTATCC 1 cut(s) 841
BglI GCCNNNNNGGC 1 cut(s) 1016
BisI GCNGC 3 cut(s) 6, 338, 1206
BlpI GCTNAGC 2 cut(s) 333, 777
BlsI GCNGC 3 cut(s) 7, 339, 1207
Bme1390I CCNGG 5 cut(s) 80, 445, 446, 761, 815
Bme18I GGWCC 1 cut(s) 811
BmeT110I CYCGRG 1 cut(s) 444
BmgT120I GGNCC 4 cut(s) 122, 811, 1222, 1327
BmiI GGNNCC 4 cut(s) 812, 813, 1297, 1328
BmrFI CCNGG 5 cut(s) 80, 445, 446, 761, 815
BmsI GCATC 1 cut(s) 319
BpiI GAAGAC 2 cut(s) 96, 530
BpmI CTGGAG 3 cut(s) 293, 395, 767
Bpu10I CCTNAGC 2 cut(s) 161, 564
Bpu1102I GCTNAGC 2 cut(s) 333, 777
BpuMI CCSGG 4 cut(s) 80, 445, 446, 815
Bsa29I ATCGAT 1 cut(s) 471
BsaAI YACGTR 1 cut(s) 362
BsaJI CCNNGG 4 cut(s) 444, 647, 759, 1209
BsaXI ACNNNNNCTCC 6 cut(s) 560, 590, 907, 937, 1181, 1211
Bsc4I CCNNNNNNNGG 6 cut(s) 60, 61, 85, 115, 1123, 1292
Bse118I RCCGGY 2 cut(s) 365, 715
Bse1I ACTGG 4 cut(s) 98, 378, 676, 701
BseBI CCWGG 1 cut(s) 761
BseCI ATCGAT 1 cut(s) 471
BseDI CCNNGG 4 cut(s) 444, 647, 759, 1209
BseGI GGATG 2 cut(s) 124, 1330
BseLI CCNNNNNNNGG 6 cut(s) 60, 61, 85, 115, 1123, 1292
BseMII CTCAG 6 cut(s) 175, 324, 450, 534, 555, 768
BseNI ACTGG 4 cut(s) 98, 378, 676, 701
BseRI GAGGAG 3 cut(s) 98, 581, 1246
BseXI GCAGC 1 cut(s) 349
Bsh1236I CGCG 1 cut(s) 20
Bsh1285I CGRYCG 1 cut(s) 116
BshFI GGCC 6 cut(s) 8, 123, 1010, 1208, 1223, 1329
BshNI GGYRCC 1 cut(s) 1295
BshVI ATCGAT 1 cut(s) 471
BsiEI CGRYCG 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 1 cut(s) 444
BsiSI CCGG 5 cut(s) 80, 366, 445, 716, 815
BslFI GGGAC 3 cut(s) 62, 797, 1147
BslI CCNNNNNNNGG 6 cut(s) 60, 61, 85, 115, 1123, 1292
BsmAI GTCTC 2 cut(s) 309, 683
BsmFI GGGAC 3 cut(s) 62, 797, 1147
BsnI GGCC 6 cut(s) 8, 123, 1010, 1208, 1223, 1329
BsoBI CYCGRG 1 cut(s) 444
Bsp1286I GDGCHC 2 cut(s) 553, 783
Bsp1407I TGTACA 1 cut(s) 658
Bsp143I GATC 7 cut(s) 113, 144, 154, 171, 468, 955, 1000
Bsp1720I GCTNAGC 2 cut(s) 333, 777
Bsp19I CCATGG 1 cut(s) 647
Bsp68I TCGCGA 1 cut(s) 20
BspACI CCGC 5 cut(s) 5, 83, 135, 926, 1205
BspANI GGCC 6 cut(s) 8, 123, 1010, 1208, 1223, 1329
BspCNI CTCAG 6 cut(s) 174, 325, 451, 535, 556, 769
BspDI ATCGAT 1 cut(s) 471
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 4 cut(s) 812, 813, 1297, 1328
BspPI GGATC 3 cut(s) 162, 166, 950
BspT107I GGYRCC 1 cut(s) 1295
BsrBI CCGCTC 1 cut(s) 137
BsrFI RCCGGY 2 cut(s) 365, 715
BsrGI TGTACA 1 cut(s) 658
BsrI ACTGG 4 cut(s) 98, 378, 676, 701
BssAI RCCGGY 2 cut(s) 365, 715
BssECI CCNNGG 4 cut(s) 444, 647, 759, 1209
BssMI GATC 7 cut(s) 113, 144, 154, 171, 468, 955, 1000
BssT1I CCWWGG 1 cut(s) 647
Bst2UI CCWGG 1 cut(s) 761
Bst4CI ACNGT 3 cut(s) 853, 1015, 1138
Bst6I CTCTTC 2 cut(s) 234, 1148
BstAPI GCANNNNNTGC 1 cut(s) 1079
BstAUI TGTACA 1 cut(s) 658
BstBAI YACGTR 1 cut(s) 362
BstC8I GCNNGC 4 cut(s) 18, 549, 654, 989
BstDEI CTNAG 7 cut(s) 161, 168, 333, 459, 543, 564, 777
BstDSI CCRYGG 1 cut(s) 647
BstF5I GGATG 2 cut(s) 124, 1330
BstFNI CGCG 1 cut(s) 20
BstHHI GCGC 1 cut(s) 943
BstKTI GATC 7 cut(s) 116, 147, 157, 174, 471, 958, 1003
BstMAI GTCTC 2 cut(s) 309, 683
BstMBI GATC 7 cut(s) 113, 144, 154, 171, 468, 955, 1000
BstMCI CGRYCG 1 cut(s) 116
BstMWI GCNNNNNNNGC 7 cut(s) 103, 455, 1016, 1079, 1156, 1211, 1220
BstNI CCWGG 1 cut(s) 761
BstNSI RCATGY 1 cut(s) 932
BstSCI CCNGG 5 cut(s) 78, 443, 444, 759, 813
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 1 cut(s) 349
BstV2I GAAGAC 2 cut(s) 96, 530
BstX2I RGATCY 1 cut(s) 171
BstYI RGATCY 1 cut(s) 171
Bsu15I ATCGAT 1 cut(s) 471
BsuI GTATCC 1 cut(s) 841
BsuRI GGCC 6 cut(s) 8, 123, 1010, 1208, 1223, 1329
BsuTUI ATCGAT 1 cut(s) 471
BtgI CCRYGG 1 cut(s) 647
BtsCI GGATG 2 cut(s) 124, 1330
BtsI GCAGTG 2 cut(s) 677, 726
BtsIMutI CAGTG 5 cut(s) 105, 677, 694, 726, 1011
BtuMI TCGCGA 1 cut(s) 20
Cac8I GCNNGC 4 cut(s) 18, 549, 654, 989
CfoI GCGC 1 cut(s) 943
Cfr10I RCCGGY 2 cut(s) 365, 715
Cfr13I GGNCC 4 cut(s) 122, 811, 1222, 1327
Cfr9I CCCGGG 1 cut(s) 444
ClaI ATCGAT 1 cut(s) 471
CseI GACGC 1 cut(s) 77
Csp6I GTAC 2 cut(s) 659, 1296
CviQI GTAC 2 cut(s) 659, 1296
DdeI CTNAG 7 cut(s) 161, 168, 333, 459, 543, 564, 777
DpnI GATC 7 cut(s) 115, 146, 156, 173, 470, 957, 1002
DpnII GATC 7 cut(s) 113, 144, 154, 171, 468, 955, 1000
DraI TTTAAA 1 cut(s) 486
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 1008
Eam1104I CTCTTC 2 cut(s) 234, 1148
EarI CTCTTC 2 cut(s) 234, 1148
EciI GGCGGA 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 647
Eco24I GRGCYC 1 cut(s) 783
Eco47I GGWCC 1 cut(s) 811
Eco57I CTGAAG 3 cut(s) 57, 161, 1172
Eco72I CACGTG 1 cut(s) 362
Eco88I CYCGRG 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 811
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 759
EcoT14I CCWWGG 1 cut(s) 647
EcoT38I GRGCYC 1 cut(s) 783
ErhI CCWWGG 1 cut(s) 647
FaqI GGGAC 3 cut(s) 62, 797, 1147
FbaI TGATCA 1 cut(s) 1000
Fnu4HI GCNGC 3 cut(s) 6, 338, 1206
FokI GGATG 2 cut(s) 131, 1317
FriOI GRGCYC 1 cut(s) 783
Fsp4HI GCNGC 3 cut(s) 6, 338, 1206
FspBI CTAG 3 cut(s) 182, 686, 920
GlaI GCGC 1 cut(s) 942
GluI GCNGC 3 cut(s) 6, 338, 1206
GsuI CTGGAG 3 cut(s) 293, 395, 767
HaeIII GGCC 6 cut(s) 8, 123, 1010, 1208, 1223, 1329
HapII CCGG 5 cut(s) 80, 366, 445, 716, 815
HgaI GACGC 1 cut(s) 77
HhaI GCGC 1 cut(s) 943
Hin6I GCGC 1 cut(s) 941
HinP1I GCGC 1 cut(s) 941
HinfI GANTC 4 cut(s) 22, 128, 302, 640
HpaII CCGG 5 cut(s) 80, 366, 445, 716, 815
HphI GGTGA 3 cut(s) 209, 380, 853
Hpy166II GTNNAC 1 cut(s) 371
Hpy188I TCNGA 6 cut(s) 113, 118, 141, 271, 738, 1247
Hpy188III TCNNGA 7 cut(s) 19, 260, 310, 686, 746, 968, 1334
Hpy8I GTNNAC 1 cut(s) 371
Hpy99I CGWCG 1 cut(s) 1314
HpyAV CCTTC 5 cut(s) 238, 343, 733, 838, 1063
HpyCH4III ACNGT 3 cut(s) 853, 1015, 1138
HpyCH4IV ACGT 3 cut(s) 361, 418, 1312
HpyCH4V TGCA 7 cut(s) 289, 359, 628, 670, 731, 881, 1106
HpyF10VI GCNNNNNNNGC 7 cut(s) 103, 455, 1016, 1079, 1156, 1211, 1220
HpyF3I CTNAG 7 cut(s) 161, 168, 333, 459, 543, 564, 777
HpySE526I ACGT 3 cut(s) 361, 418, 1312
HspAI GCGC 1 cut(s) 941
KflI GGGWCCC 1 cut(s) 811
KpnI GGTACC 1 cut(s) 1299
Ksp22I TGATCA 1 cut(s) 1000
Kzo9I GATC 7 cut(s) 113, 144, 154, 171, 468, 955, 1000
LmnI GCTCC 4 cut(s) 56, 111, 632, 928
Lsp1109I GCAGC 1 cut(s) 349
LweI GCATC 1 cut(s) 319
MaeI CTAG 3 cut(s) 182, 686, 920
MaeII ACGT 3 cut(s) 361, 418, 1312
MalI GATC 7 cut(s) 115, 146, 156, 173, 470, 957, 1002
MbiI CCGCTC 1 cut(s) 137
MboI GATC 7 cut(s) 113, 144, 154, 171, 468, 955, 1000
MflI RGATCY 1 cut(s) 171
MhlI GDGCHC 2 cut(s) 553, 783
MlsI TGGCCA 1 cut(s) 1010
MluCI AATT 7 cut(s) 45, 185, 404, 732, 1100, 1236, 1248
MluNI TGGCCA 1 cut(s) 1010
MlyI GAGTC 1 cut(s) 311
MmeI TCCRAC 2 cut(s) 132, 1173
Mox20I TGGCCA 1 cut(s) 1010
MscI TGGCCA 1 cut(s) 1010
MseI TTAA 9 cut(s) 207, 387, 396, 485, 555, 578, 617, 797, 875
MslI CAYNNNNRTG 1 cut(s) 360
Msp20I TGGCCA 1 cut(s) 1010
MspA1I CMGCKG 1 cut(s) 340
MspI CCGG 5 cut(s) 80, 366, 445, 716, 815
MspR9I CCNGG 5 cut(s) 80, 445, 446, 761, 815
MvaI CCWGG 1 cut(s) 761
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 7 cut(s) 103, 455, 1016, 1079, 1156, 1211, 1220
NciI CCSGG 4 cut(s) 80, 445, 446, 815
NcoI CCATGG 1 cut(s) 647
NdeII GATC 7 cut(s) 113, 144, 154, 171, 468, 955, 1000
NlaIV GGNNCC 4 cut(s) 812, 813, 1297, 1328
NmeAIII GCCGAG 1 cut(s) 1190
NruI TCGCGA 1 cut(s) 20
NspI RCATGY 1 cut(s) 932
PfeI GAWTC 3 cut(s) 22, 128, 640
PkrI GCNGC 3 cut(s) 7, 339, 1207
Ple19I CGATCG 1 cut(s) 116
PleI GAGTC 1 cut(s) 310
PmaCI CACGTG 1 cut(s) 362
PmlI CACGTG 1 cut(s) 362
PpsI GAGTC 1 cut(s) 310
Ppu21I YACGTR 1 cut(s) 362
PpuMI RGGWCCY 1 cut(s) 811
Psp5II RGGWCCY 1 cut(s) 811
Psp6I CCWGG 1 cut(s) 759
PspCI CACGTG 1 cut(s) 362
PspGI CCWGG 1 cut(s) 759
PspN4I GGNNCC 4 cut(s) 812, 813, 1297, 1328
PspPI GGNCC 4 cut(s) 122, 811, 1222, 1327
PspPPI RGGWCCY 1 cut(s) 811
PsuI RGATCY 1 cut(s) 171
PvuI CGATCG 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 340
RruI TCGCGA 1 cut(s) 20
RsaI GTAC 2 cut(s) 660, 1297
RsaNI GTAC 2 cut(s) 659, 1296
RseI CAYNNNNRTG 1 cut(s) 360
SaqAI TTAA 9 cut(s) 207, 387, 396, 485, 555, 578, 617, 797, 875
SatI GCNGC 3 cut(s) 6, 338, 1206
Sau3AI GATC 7 cut(s) 113, 144, 154, 171, 468, 955, 1000
Sau96I GGNCC 4 cut(s) 122, 811, 1222, 1327
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 5 cut(s) 80, 445, 446, 761, 815
SduI GDGCHC 2 cut(s) 553, 783
SfaNI GCATC 1 cut(s) 319
SinI GGWCC 1 cut(s) 811
SmaI CCCGGG 1 cut(s) 446
SmiMI CAYNNNNRTG 1 cut(s) 360
SrfI GCCCGGGC 1 cut(s) 446
Sse9I AATT 7 cut(s) 45, 185, 404, 732, 1100, 1236, 1248
SsiI CCGC 5 cut(s) 5, 83, 135, 926, 1205
SspMI CTAG 3 cut(s) 182, 686, 920
StyD4I CCNGG 5 cut(s) 78, 443, 444, 759, 813
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 3 cut(s) 853, 1015, 1138
TaiI ACGT 3 cut(s) 364, 421, 1315
TaqI TCGA 6 cut(s) 147, 189, 300, 471, 1042, 1291
TasI AATT 7 cut(s) 45, 185, 404, 732, 1100, 1236, 1248
TatI WGTACW 1 cut(s) 658
TauI GCSGC 2 cut(s) 8, 1208
TfiI GAWTC 3 cut(s) 22, 128, 640
Tru1I TTAA 9 cut(s) 207, 387, 396, 485, 555, 578, 617, 797, 875
Tru9I TTAA 9 cut(s) 207, 387, 396, 485, 555, 578, 617, 797, 875
TscAI CASTG 5 cut(s) 105, 677, 701, 726, 1018
TseI GCWGC 1 cut(s) 337
TspDTI ATGAA 5 cut(s) 405, 468, 695, 915, 1176
TspGWI ACGGA 1 cut(s) 524
TspMI CCCGGG 1 cut(s) 444
TspRI CASTG 5 cut(s) 105, 677, 701, 726, 1018
VpaK11BI GGWCC 1 cut(s) 811
XapI RAATTY 3 cut(s) 185, 1100, 1248
XbaI TCTAGA 1 cut(s) 685
XceI RCATGY 1 cut(s) 932
XmaI CCCGGG 1 cut(s) 444
XspI CTAG 3 cut(s) 182, 686, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.