MD15G1100200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007104
Physical Location & Seq
Reverse (-)
6963724 .. 6968556
4833 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1100200.v1.1.491

Sequence Viewer

Length: 1338 bp
ATGGCGGCCTCCAAGCTCGCGATTCTCTCAATCTTTCTTGCCCTTATTTTCTCCCAGATTCGGGCTGACGCTTCAGTCCCGGCGGAGGAAGACGAGCCAGTGAAGCTCCCCCGATCGGACGGCCCCGATTCGTCCGCTCTGAAGATCGAGTTGGATCAGCTCAGGGCTAAGATCCACTCCCTAGAATTCGATGTCGGTGAGAAGATTAAGGAACTAAAAAAGAAGAATGAGCTTATAGAAGAGAAGGAGAAAGTAATCCAAAAGAACTCGGATAGCATTTCTTCGTTGCAGAGTGAAATTGATTCTCTCCAGAAAAAAGGGTCATTAGATGCTGAGCTTGGGAAGGCTCATGCACGTGCTGGTGAACTAGAGAAAAAGGTTAATGAACTTAAAAAGGAATTGCAATCGCAACGTAATGAGAAAGAGGGCTTGGAAGCCCGGGCGAATGAAGCTGAGAAGAAGGTCGATGTTTTGCTTTTGAAAATAGAGAATATTGAGAAGACTAATGATGAGCAGAAGACCAAAATCCGTAAAACTGAGCGTGCTCTTAAAGTGGCTGAGGAGGAAATGTTGAAGGCAAAGTTTGAAGCTACTCTAAAAGCAAAAGAGTTAAATGAGGTGCATGGTGCATGGATTCCACCATGGCTTGCTGTACATTTTATGCAGTGTCAGTCCTTTCTAGAGAGACACTGGAATGTGCATGGAAAACCTGCAGTGGATATTGCTGTTCAGAAGGCTCTGGAGAAAAAAGCCCAGGCTGAAAAGTGGGCTGAACCCCATGTGGAAACATTAAAAACTAAATGGATCCCGGCAATCAAGGAACAATGGGAGGTGGTGAAGGATACTGTTGAACCTCATGTGAAATCGTTAAGTACAAAAACTTTTGAAGTTTATGAGGTTTCAAAGACTACACTAGCTCCACACGTTATAAAAGCACAAGAAGTTGTTGATCCTTATTTTCAGGAAGCAAAAAAGTTCAGCAAGCCATATGTTGATCAGGTGGCCACTGTGGCAAAACCTCATGTCGATAAGGTTCGAGTGGTATTGAAGCCTTATACAAAGAAGGCAGTTATTGCCTACGAGAAGTTTCTCAAATCTGCAACAGCATACCATCATCAGGTTCAAGGGACGGTAAAAGATTTGCTGAAGAGGCATGAACTGACAAGACCTCTTGCTACAAAGGAGTTGGAGTGGTTTGCGGCCTCGGCTTTGTTGGCCCTGCCCATTATCATTCTGTTCAGAATTTTCTCCTCACTTTTCTGTACAAAGACTAAAAACCCTGTTCGAGGTACCAACCATACACGTCGTAAGGCTAAAAGGGGCCATCCAGAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

446

Amino Acids

50.63

Weight (kDa)

9.31

Isoelectric Point (pI)

30.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4686 PF15742 44 - 205 4.2e-06 Domain of unknown function (DUF4686)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 929
AasI GACNNNNNNGTC 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 718
Acc65I GGTACC 1 cut(s) 1289
AccB1I GGYRCC 1 cut(s) 1289
AccBSI CCGCTC 1 cut(s) 137
AccII CGCG 1 cut(s) 20
AciI CCGC 4 cut(s) 5, 83, 135, 1199
AclWI GGATC 5 cut(s) 162, 166, 799, 812, 944
AcoI YGGCCR 1 cut(s) 1002
AcsI RAATTY 2 cut(s) 185, 1242
AcuI CTGAAG 3 cut(s) 57, 161, 1166
AcvI CACGTG 1 cut(s) 356
AfaI GTAC 4 cut(s) 654, 874, 1264, 1291
AfiI CCNNNNNNNGG 6 cut(s) 60, 61, 85, 115, 1117, 1286
AflIII ACRYGT 2 cut(s) 922, 1301
AgsI TTSAA 8 cut(s) 481, 574, 587, 851, 887, 903, 1048, 1124
AjiI CACGTC 1 cut(s) 1304
AjnI CCWGG 1 cut(s) 753
AjuI GAANNNNNNNTTGG 4 cut(s) 134, 166, 413, 445
AluBI AGCT 8 cut(s) 16, 106, 160, 232, 337, 452, 590, 917
AluI AGCT 8 cut(s) 16, 106, 160, 232, 337, 452, 590, 917
Alw21I GWGCWC 1 cut(s) 547
Alw26I GTCTC 1 cut(s) 679
AlwI GGATC 5 cut(s) 162, 166, 799, 812, 944
Ama87I CYCGRG 1 cut(s) 438
AoxI GGCC 6 cut(s) 6, 121, 1002, 1200, 1215, 1321
ApoI RAATTY 2 cut(s) 185, 1242
Asp718I GGTACC 1 cut(s) 1289
AspS9I GGNCC 3 cut(s) 122, 1216, 1321
AsuC2I CCSGG 4 cut(s) 80, 439, 440, 809
AsuHPI GGTGA 3 cut(s) 209, 374, 847
AvaI CYCGRG 1 cut(s) 438
BalI TGGCCA 1 cut(s) 1004
BamHI GGATCC 1 cut(s) 804
BanI GGYRCC 1 cut(s) 1289
BbrPI CACGTG 1 cut(s) 356
BbsI GAAGAC 3 cut(s) 96, 506, 524
Bbv12I GWGCWC 1 cut(s) 547
BbvCI CCTCAGC 1 cut(s) 558
BccI CCATC 2 cut(s) 1119, 1332
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 1 cut(s) 755
BciVI GTATCC 1 cut(s) 835
BclI TGATCA 1 cut(s) 994
BcnI CCSGG 4 cut(s) 80, 439, 440, 809
BcoDI GTCTC 1 cut(s) 679
BfaI CTAG 4 cut(s) 182, 368, 680, 914
BfmI CTRYAG 1 cut(s) 711
BfuAI ACCTGC 1 cut(s) 718
BfuI GTATCC 1 cut(s) 835
BglI GCCNNNNNGGC 1 cut(s) 1010
BisI GCNGC 2 cut(s) 6, 1200
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 2 cut(s) 7, 1201
Bme1390I CCNGG 5 cut(s) 80, 439, 440, 755, 809
BmeT110I CYCGRG 1 cut(s) 438
BmgBI CACGTC 1 cut(s) 1304
BmgT120I GGNCC 3 cut(s) 122, 1216, 1321
BmiI GGNNCC 4 cut(s) 124, 806, 1291, 1322
BmrFI CCNGG 5 cut(s) 80, 439, 440, 755, 809
BmsI GCATC 1 cut(s) 319
BpiI GAAGAC 3 cut(s) 96, 506, 524
BpmI CTGGAG 2 cut(s) 293, 761
Bpu10I CCTNAGC 2 cut(s) 161, 558
Bpu1102I GCTNAGC 1 cut(s) 333
BpuMI CCSGG 4 cut(s) 80, 439, 440, 809
BsaAI YACGTR 1 cut(s) 356
BsaJI CCNNGG 4 cut(s) 438, 641, 753, 1203
BsaXI ACNNNNNCTCC 6 cut(s) 554, 584, 901, 931, 1175, 1205
Bsc4I CCNNNNNNNGG 6 cut(s) 60, 61, 85, 115, 1117, 1286
Bse1I ACTGG 2 cut(s) 98, 695
BseBI CCWGG 1 cut(s) 755
BseDI CCNNGG 4 cut(s) 438, 641, 753, 1203
BseGI GGATG 1 cut(s) 1324
BseLI CCNNNNNNNGG 6 cut(s) 60, 61, 85, 115, 1117, 1286
BseMII CTCAG 5 cut(s) 175, 324, 444, 528, 549
BseNI ACTGG 2 cut(s) 98, 695
BseRI GAGGAG 2 cut(s) 575, 1240
Bsh1236I CGCG 1 cut(s) 20
Bsh1285I CGRYCG 1 cut(s) 116
BshFI GGCC 6 cut(s) 8, 123, 1004, 1202, 1217, 1323
BshNI GGYRCC 1 cut(s) 1289
BsiEI CGRYCG 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 547
BsiHKCI CYCGRG 1 cut(s) 438
BsiSI CCGG 3 cut(s) 80, 439, 809
BslFI GGGAC 2 cut(s) 62, 1141
BslI CCNNNNNNNGG 6 cut(s) 60, 61, 85, 115, 1117, 1286
BsmAI GTCTC 1 cut(s) 679
BsmFI GGGAC 2 cut(s) 62, 1141
BsnI GGCC 6 cut(s) 8, 123, 1004, 1202, 1217, 1323
BsoBI CYCGRG 1 cut(s) 438
Bsp1286I GDGCHC 1 cut(s) 547
Bsp1407I TGTACA 2 cut(s) 652, 1262
Bsp143I GATC 7 cut(s) 113, 144, 154, 171, 804, 949, 994
Bsp1720I GCTNAGC 1 cut(s) 333
Bsp19I CCATGG 1 cut(s) 641
Bsp68I TCGCGA 1 cut(s) 20
BspACI CCGC 4 cut(s) 5, 83, 135, 1199
BspANI GGCC 6 cut(s) 8, 123, 1004, 1202, 1217, 1323
BspCNI CTCAG 5 cut(s) 174, 325, 445, 529, 550
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 4 cut(s) 124, 806, 1291, 1322
BspMAI CTGCAG 1 cut(s) 715
BspMI ACCTGC 1 cut(s) 718
BspPI GGATC 5 cut(s) 162, 166, 799, 812, 944
BspT107I GGYRCC 1 cut(s) 1289
BsrBI CCGCTC 1 cut(s) 137
BsrGI TGTACA 2 cut(s) 652, 1262
BsrI ACTGG 2 cut(s) 98, 695
BssECI CCNNGG 4 cut(s) 438, 641, 753, 1203
BssMI GATC 7 cut(s) 113, 144, 154, 171, 804, 949, 994
BssT1I CCWWGG 1 cut(s) 641
Bst2UI CCWGG 1 cut(s) 755
Bst4CI ACNGT 3 cut(s) 847, 1009, 1132
Bst6I CTCTTC 2 cut(s) 234, 1142
BstAPI GCANNNNNTGC 1 cut(s) 1073
BstAUI TGTACA 2 cut(s) 652, 1262
BstBAI YACGTR 1 cut(s) 356
BstC8I GCNNGC 4 cut(s) 18, 543, 648, 983
BstDEI CTNAG 6 cut(s) 161, 168, 333, 453, 537, 558
BstDSI CCRYGG 1 cut(s) 641
BstENI CCTNNNNNAGG 1 cut(s) 1284
BstF5I GGATG 1 cut(s) 1324
BstFNI CGCG 1 cut(s) 20
BstKTI GATC 7 cut(s) 116, 147, 157, 174, 807, 952, 997
BstMAI GTCTC 1 cut(s) 679
BstMBI GATC 7 cut(s) 113, 144, 154, 171, 804, 949, 994
BstMCI CGRYCG 1 cut(s) 116
BstMWI GCNNNNNNNGC 7 cut(s) 103, 449, 1010, 1073, 1150, 1205, 1214
BstNI CCWGG 1 cut(s) 755
BstSCI CCNGG 5 cut(s) 78, 437, 438, 753, 807
BstSFI CTRYAG 1 cut(s) 711
BstUI CGCG 1 cut(s) 20
BstV2I GAAGAC 3 cut(s) 96, 506, 524
BstX2I RGATCY 2 cut(s) 171, 804
BstYI RGATCY 2 cut(s) 171, 804
BsuI GTATCC 1 cut(s) 835
BsuRI GGCC 6 cut(s) 8, 123, 1004, 1202, 1217, 1323
BtgI CCRYGG 1 cut(s) 641
BtrI CACGTC 1 cut(s) 1304
BtsCI GGATG 1 cut(s) 1324
BtsI GCAGTG 2 cut(s) 671, 720
BtsIMutI CAGTG 5 cut(s) 105, 671, 688, 720, 1005
BtuMI TCGCGA 1 cut(s) 20
BveI ACCTGC 1 cut(s) 718
Cac8I GCNNGC 4 cut(s) 18, 543, 648, 983
Cfr13I GGNCC 3 cut(s) 122, 1216, 1321
Cfr9I CCCGGG 1 cut(s) 438
CseI GACGC 1 cut(s) 77
Csp6I GTAC 4 cut(s) 653, 873, 1263, 1290
CviAII CATG 9 cut(s) 350, 623, 630, 642, 701, 779, 857, 1022, 1154
CviQI GTAC 4 cut(s) 653, 873, 1263, 1290
DdeI CTNAG 6 cut(s) 161, 168, 333, 453, 537, 558
DpnI GATC 7 cut(s) 115, 146, 156, 173, 806, 951, 996
DpnII GATC 7 cut(s) 113, 144, 154, 171, 804, 949, 994
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 1002
Eam1104I CTCTTC 2 cut(s) 234, 1142
EarI CTCTTC 2 cut(s) 234, 1142
EciI GGCGGA 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 641
Eco57I CTGAAG 3 cut(s) 57, 161, 1166
Eco72I CACGTG 1 cut(s) 356
Eco88I CYCGRG 1 cut(s) 438
EcoNI CCTNNNNNAGG 1 cut(s) 1284
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 753
EcoT14I CCWWGG 1 cut(s) 641
ErhI CCWWGG 1 cut(s) 641
FaeI CATG 9 cut(s) 353, 626, 633, 645, 704, 782, 860, 1025, 1157
FaqI GGGAC 2 cut(s) 62, 1141
FatI CATG 9 cut(s) 349, 622, 629, 641, 700, 778, 856, 1021, 1153
FauNDI CATATG 1 cut(s) 988
FbaI TGATCA 1 cut(s) 994
Fnu4HI GCNGC 2 cut(s) 6, 1200
FokI GGATG 1 cut(s) 1311
Fsp4HI GCNGC 2 cut(s) 6, 1200
FspBI CTAG 4 cut(s) 182, 368, 680, 914
GluI GCNGC 2 cut(s) 6, 1200
GsuI CTGGAG 2 cut(s) 293, 761
HaeIII GGCC 6 cut(s) 8, 123, 1004, 1202, 1217, 1323
HapII CCGG 3 cut(s) 80, 439, 809
HgaI GACGC 1 cut(s) 77
Hin1II CATG 9 cut(s) 353, 626, 633, 645, 704, 782, 860, 1025, 1157
HinfI GANTC 5 cut(s) 22, 58, 128, 302, 634
HpaII CCGG 3 cut(s) 80, 439, 809
HphI GGTGA 3 cut(s) 209, 374, 847
Hpy166II GTNNAC 1 cut(s) 365
Hpy188I TCNGA 5 cut(s) 118, 141, 271, 732, 1241
Hpy188III TCNNGA 6 cut(s) 19, 310, 680, 740, 962, 1328
Hpy8I GTNNAC 1 cut(s) 365
Hpy99I CGWCG 1 cut(s) 1308
HpyAV CCTTC 7 cut(s) 238, 337, 454, 568, 727, 832, 1057
HpyCH4III ACNGT 3 cut(s) 847, 1009, 1132
HpyCH4IV ACGT 4 cut(s) 355, 412, 924, 1303
HpyCH4V TGCA 9 cut(s) 289, 353, 403, 622, 629, 664, 700, 713, 1100
HpyF10VI GCNNNNNNNGC 7 cut(s) 103, 449, 1010, 1073, 1150, 1205, 1214
HpyF3I CTNAG 6 cut(s) 161, 168, 333, 453, 537, 558
HpySE526I ACGT 4 cut(s) 355, 412, 924, 1303
Hsp92II CATG 9 cut(s) 353, 626, 633, 645, 704, 782, 860, 1025, 1157
KpnI GGTACC 1 cut(s) 1293
Ksp22I TGATCA 1 cut(s) 994
Kzo9I GATC 7 cut(s) 113, 144, 154, 171, 804, 949, 994
LmnI GCTCC 2 cut(s) 111, 922
LweI GCATC 1 cut(s) 319
MaeI CTAG 4 cut(s) 182, 368, 680, 914
MaeII ACGT 4 cut(s) 355, 412, 924, 1303
MalI GATC 7 cut(s) 115, 146, 156, 173, 806, 951, 996
MbiI CCGCTC 1 cut(s) 137
MboI GATC 7 cut(s) 113, 144, 154, 171, 804, 949, 994
MflI RGATCY 2 cut(s) 171, 804
MhlI GDGCHC 1 cut(s) 547
MlsI TGGCCA 1 cut(s) 1004
MluCI AATT 4 cut(s) 185, 297, 398, 1242
MluNI TGGCCA 1 cut(s) 1004
MmeI TCCRAC 2 cut(s) 132, 1167
Mox20I TGGCCA 1 cut(s) 1004
MscI TGGCCA 1 cut(s) 1004
MseI TTAA 7 cut(s) 207, 381, 390, 549, 611, 791, 869
MslI CAYNNNNRTG 2 cut(s) 354, 693
Msp20I TGGCCA 1 cut(s) 1004
MspI CCGG 3 cut(s) 80, 439, 809
MspR9I CCNGG 5 cut(s) 80, 439, 440, 755, 809
MvaI CCWGG 1 cut(s) 755
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 7 cut(s) 103, 449, 1010, 1073, 1150, 1205, 1214
NciI CCSGG 4 cut(s) 80, 439, 440, 809
NcoI CCATGG 1 cut(s) 641
NdeI CATATG 1 cut(s) 988
NdeII GATC 7 cut(s) 113, 144, 154, 171, 804, 949, 994
NlaIII CATG 9 cut(s) 353, 626, 633, 645, 704, 782, 860, 1025, 1157
NlaIV GGNNCC 4 cut(s) 124, 806, 1291, 1322
NmeAIII GCCGAG 1 cut(s) 1184
NruI TCGCGA 1 cut(s) 20
PfeI GAWTC 5 cut(s) 22, 58, 128, 302, 634
PkrI GCNGC 2 cut(s) 7, 1201
Ple19I CGATCG 1 cut(s) 116
PmaCI CACGTG 1 cut(s) 356
PmlI CACGTG 1 cut(s) 356
Ppu21I YACGTR 1 cut(s) 356
PsiI TTATAA 1 cut(s) 929
Psp6I CCWGG 1 cut(s) 753
PspCI CACGTG 1 cut(s) 356
PspGI CCWGG 1 cut(s) 753
PspN4I GGNNCC 4 cut(s) 124, 806, 1291, 1322
PspPI GGNCC 3 cut(s) 122, 1216, 1321
PstI CTGCAG 1 cut(s) 715
PsuI RGATCY 2 cut(s) 171, 804
PvuI CGATCG 1 cut(s) 116
RruI TCGCGA 1 cut(s) 20
RsaI GTAC 4 cut(s) 654, 874, 1264, 1291
RsaNI GTAC 4 cut(s) 653, 873, 1263, 1290
RseI CAYNNNNRTG 2 cut(s) 354, 693
SaqAI TTAA 7 cut(s) 207, 381, 390, 549, 611, 791, 869
SatI GCNGC 2 cut(s) 6, 1200
Sau3AI GATC 7 cut(s) 113, 144, 154, 171, 804, 949, 994
Sau96I GGNCC 3 cut(s) 122, 1216, 1321
ScrFI CCNGG 5 cut(s) 80, 439, 440, 755, 809
SduI GDGCHC 1 cut(s) 547
SfaNI GCATC 1 cut(s) 319
SfcI CTRYAG 1 cut(s) 711
SmaI CCCGGG 1 cut(s) 440
SmiMI CAYNNNNRTG 2 cut(s) 354, 693
SrfI GCCCGGGC 1 cut(s) 440
Sse9I AATT 4 cut(s) 185, 297, 398, 1242
SsiI CCGC 4 cut(s) 5, 83, 135, 1199
SspI AATATT 1 cut(s) 493
SspMI CTAG 4 cut(s) 182, 368, 680, 914
StyD4I CCNGG 5 cut(s) 78, 437, 438, 753, 807
StyI CCWWGG 1 cut(s) 641
TaaI ACNGT 3 cut(s) 847, 1009, 1132
TaiI ACGT 4 cut(s) 358, 415, 927, 1306
TaqI TCGA 6 cut(s) 147, 189, 465, 1026, 1036, 1285
TasI AATT 4 cut(s) 185, 297, 398, 1242
TatI WGTACW 3 cut(s) 652, 872, 1262
TauI GCSGC 2 cut(s) 8, 1202
TfiI GAWTC 5 cut(s) 22, 58, 128, 302, 634
Tru1I TTAA 7 cut(s) 207, 381, 390, 549, 611, 791, 869
Tru9I TTAA 7 cut(s) 207, 381, 390, 549, 611, 791, 869
TscAI CASTG 5 cut(s) 105, 671, 695, 720, 1012
TspDTI ATGAA 3 cut(s) 399, 462, 1170
TspGWI ACGGA 1 cut(s) 518
TspMI CCCGGG 1 cut(s) 438
TspRI CASTG 5 cut(s) 105, 671, 695, 720, 1012
XagI CCTNNNNNAGG 1 cut(s) 1284
XapI RAATTY 2 cut(s) 185, 1242
XbaI TCTAGA 1 cut(s) 679
XmaI CCCGGG 1 cut(s) 438
XspI CTAG 4 cut(s) 182, 368, 680, 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.