pycom08g10130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0007104
Physical Location & Seq
Reverse (-)
8440762 .. 8445144
4383 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g10130.6

Sequence Viewer

Length: 1272 bp
ATGGCGGCCTCCAAGCTCGCGATTCTCTCAATCTTTCTTGCCCTAATTTGCTCCCAGATTAGCGCTGACGCTTCAGTCCCGGCGGAGGAAGACGAGCCAGTGAAGCTCCTCCGATCGGACGGCCCTGATTCGTCCGCTCTGAAGATCGAGTTGGATCAGCTCAGGGCTAAGATCCACTCCCTAGAATTCGAGGTCGGTGAGAAGATTAAGGAACTAAAAAGGAAGAATGAGCTTATAGAAGAGAAGGAGAAAGTCATTCAAGAGAACTCGGATAGCATTTCTTCGTTGCAGAGTGAAATCGAGTCTCTCCAGGTTAATGAACTTAAAAAGGAATTACAATCGCAACGTAAGGAGAAAGAGGGCTTGGAAGCCCGGGCAAATGAAGCTGAGAAGAAGATCGATGTTTTGCTTTTAAAAATAGGGAAGATTGAGAAGACTAATGATGAGCAGAAGACCAAAATCCGTAATACTGAGCGTGCTCTTAAAGTGGCTGAGGAGGAAATGTTAAGGGCAAAGTTTGAAGCTACTCAAAAAGCAAAAGAGTTAAATGAGGTGCATGGAGCATGGATTCCACCATGGCTTGCTGTACATTTTATGCAGTGCCAGTCCTTTCTAGAGACACACTGGAAAGTTCATGGAAAACCAGCAGTGGATATTGCAATTCAGAAGGCTCTGGAGAAAAAAGCCCAGGCTGAAAAGTGGGCTGAGCCCCATGTGGAAACATTAAAAACTAAATGGGTCCCGGCAATCAAGGAACAATGGGTGGTGGTGAAGGATACTGTTGAACCTCATGTGAAATCGTTTAGTGCAAAAACTATTGAAGTTTATGAGGTTTCAAAGACTACACTAGCTCCACATGTTCTAAAAGCGCAGGAAGTTGTTGATCCTTATTTTCAGGAAGCAAAAAGGTTCAGCAAGCCGTATGTTGATCGGGTGGCCACTGTGGCAAAACCTCATGTCGATAAGGTTCGAGTAGTATTGAAGCCTTATACAAAGAAGGCAGTTATTGCCTACGAGAAGTTTCTCAAATCTGCAACAACATACCATCATCAGGTTCAAGGGACAGTAAAAGATTTGCTGAAGAGGCATGAACTGACAAGACCTCTTGCTACAAAGGAGTTGGAGTGGTTTGCGGCCTCGGCTTTGTTGGCCCTGCCCATCATAATTCTGTTCAGAATTTTCTCCTCACTTTTCGGTACAAAGACTAAAAAACCCGTTCGAGGTACCAACCATACACGACGTAAGGTTAAAAGGGGCCATCCAGAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

424

Amino Acids

48.25

Weight (kDa)

9.35

Isoelectric Point (pI)

31.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
Acc65I GGTACC 1 cut(s) 1223
AccB1I GGYRCC 1 cut(s) 1223
AccBSI CCGCTC 1 cut(s) 137
AccII CGCG 1 cut(s) 20
AciI CCGC 4 cut(s) 5, 83, 135, 1133
AclWI GGATC 3 cut(s) 162, 166, 878
AcoI YGGCCR 1 cut(s) 936
AcsI RAATTY 2 cut(s) 185, 1176
AcuI CTGAAG 3 cut(s) 57, 161, 1100
AfaI GTAC 3 cut(s) 588, 1198, 1225
AfeI AGCGCT 1 cut(s) 64
AfiI CCNNNNNNNGG 4 cut(s) 85, 115, 1051, 1220
AflIII ACRYGT 1 cut(s) 856
AgsI TTSAA 7 cut(s) 260, 521, 785, 821, 837, 982, 1058
AjnI CCWGG 2 cut(s) 309, 687
AjuI GAANNNNNNNTTGG 4 cut(s) 134, 166, 347, 379
AluBI AGCT 7 cut(s) 16, 106, 160, 232, 386, 524, 851
AluI AGCT 7 cut(s) 16, 106, 160, 232, 386, 524, 851
Alw21I GWGCWC 1 cut(s) 481
Alw26I GTCTC 2 cut(s) 309, 611
AlwI GGATC 3 cut(s) 162, 166, 878
Ama87I CYCGRG 1 cut(s) 372
Aor51HI AGCGCT 1 cut(s) 64
AoxI GGCC 6 cut(s) 6, 121, 936, 1134, 1149, 1255
ApoI RAATTY 2 cut(s) 185, 1176
Asp718I GGTACC 1 cut(s) 1223
AspLEI GCGC 2 cut(s) 65, 871
AspS9I GGNCC 4 cut(s) 122, 739, 1150, 1255
AsuC2I CCSGG 4 cut(s) 80, 373, 374, 743
AsuHPI GGTGA 2 cut(s) 209, 781
AvaI CYCGRG 1 cut(s) 372
AvaII GGWCC 1 cut(s) 739
BalI TGGCCA 1 cut(s) 938
BanI GGYRCC 1 cut(s) 1223
BanII GRGCYC 1 cut(s) 711
BbsI GAAGAC 3 cut(s) 96, 440, 458
Bbv12I GWGCWC 1 cut(s) 481
BbvCI CCTCAGC 1 cut(s) 492
BccI CCATC 3 cut(s) 1053, 1166, 1266
BceAI ACGGC 2 cut(s) 136, 904
BciT130I CCWGG 2 cut(s) 311, 689
BciVI GTATCC 1 cut(s) 769
BcnI CCSGG 4 cut(s) 80, 373, 374, 743
BcoDI GTCTC 2 cut(s) 309, 611
BfaI CTAG 3 cut(s) 182, 614, 848
BfoI RGCGCY 1 cut(s) 66
BfuI GTATCC 1 cut(s) 769
BglI GCCNNNNNGGC 1 cut(s) 944
BisI GCNGC 2 cut(s) 6, 1134
BlpI GCTNAGC 1 cut(s) 705
BlsI GCNGC 2 cut(s) 7, 1135
Bme1390I CCNGG 6 cut(s) 80, 311, 373, 374, 689, 743
Bme18I GGWCC 1 cut(s) 739
BmeT110I CYCGRG 1 cut(s) 372
BmgT120I GGNCC 4 cut(s) 122, 739, 1150, 1255
BmiI GGNNCC 4 cut(s) 740, 741, 1225, 1256
BmrFI CCNGG 6 cut(s) 80, 311, 373, 374, 689, 743
BpiI GAAGAC 3 cut(s) 96, 440, 458
BpmI CTGGAG 2 cut(s) 293, 695
Bpu10I CCTNAGC 2 cut(s) 161, 492
Bpu1102I GCTNAGC 1 cut(s) 705
BpuMI CCSGG 4 cut(s) 80, 373, 374, 743
Bsa29I ATCGAT 1 cut(s) 399
BsaJI CCNNGG 4 cut(s) 372, 575, 687, 1137
BsaXI ACNNNNNCTCC 6 cut(s) 488, 518, 835, 865, 1109, 1139
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 115, 1051, 1220
Bse1I ACTGG 3 cut(s) 98, 604, 629
BseBI CCWGG 2 cut(s) 311, 689
BseCI ATCGAT 1 cut(s) 399
BseDI CCNNGG 4 cut(s) 372, 575, 687, 1137
BseGI GGATG 1 cut(s) 1258
BseLI CCNNNNNNNGG 4 cut(s) 85, 115, 1051, 1220
BseMII CTCAG 5 cut(s) 175, 378, 462, 483, 696
BseNI ACTGG 3 cut(s) 98, 604, 629
BseRI GAGGAG 3 cut(s) 98, 509, 1174
Bsh1236I CGCG 1 cut(s) 20
Bsh1285I CGRYCG 1 cut(s) 116
BshFI GGCC 6 cut(s) 8, 123, 938, 1136, 1151, 1257
BshNI GGYRCC 1 cut(s) 1223
BshVI ATCGAT 1 cut(s) 399
BsiEI CGRYCG 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 481
BsiHKCI CYCGRG 1 cut(s) 372
BsiSI CCGG 3 cut(s) 80, 373, 743
BslFI GGGAC 3 cut(s) 62, 725, 1075
BslI CCNNNNNNNGG 4 cut(s) 85, 115, 1051, 1220
BsmAI GTCTC 2 cut(s) 309, 611
BsmFI GGGAC 3 cut(s) 62, 725, 1075
BsnI GGCC 6 cut(s) 8, 123, 938, 1136, 1151, 1257
BsoBI CYCGRG 1 cut(s) 372
Bsp1286I GDGCHC 2 cut(s) 481, 711
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 7 cut(s) 113, 144, 154, 171, 396, 883, 928
Bsp1720I GCTNAGC 1 cut(s) 705
Bsp19I CCATGG 1 cut(s) 575
Bsp68I TCGCGA 1 cut(s) 20
BspACI CCGC 4 cut(s) 5, 83, 135, 1133
BspANI GGCC 6 cut(s) 8, 123, 938, 1136, 1151, 1257
BspCNI CTCAG 5 cut(s) 174, 379, 463, 484, 697
BspDI ATCGAT 1 cut(s) 399
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 4 cut(s) 740, 741, 1225, 1256
BspPI GGATC 3 cut(s) 162, 166, 878
BspT107I GGYRCC 1 cut(s) 1223
BsrBI CCGCTC 1 cut(s) 137
BsrGI TGTACA 1 cut(s) 586
BsrI ACTGG 3 cut(s) 98, 604, 629
BssECI CCNNGG 4 cut(s) 372, 575, 687, 1137
BssMI GATC 7 cut(s) 113, 144, 154, 171, 396, 883, 928
BssT1I CCWWGG 1 cut(s) 575
Bst2UI CCWGG 2 cut(s) 311, 689
Bst4CI ACNGT 3 cut(s) 781, 943, 1066
Bst6I CTCTTC 2 cut(s) 234, 1076
BstAPI GCANNNNNTGC 1 cut(s) 1007
BstAUI TGTACA 1 cut(s) 586
BstC8I GCNNGC 4 cut(s) 18, 477, 582, 917
BstDEI CTNAG 6 cut(s) 161, 168, 387, 471, 492, 705
BstDSI CCRYGG 1 cut(s) 575
BstF5I GGATG 1 cut(s) 1258
BstFNI CGCG 1 cut(s) 20
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 2 cut(s) 65, 871
BstKTI GATC 7 cut(s) 116, 147, 157, 174, 399, 886, 931
BstMAI GTCTC 2 cut(s) 309, 611
BstMBI GATC 7 cut(s) 113, 144, 154, 171, 396, 883, 928
BstMCI CGRYCG 1 cut(s) 116
BstMWI GCNNNNNNNGC 7 cut(s) 103, 383, 944, 1007, 1084, 1139, 1148
BstNI CCWGG 2 cut(s) 311, 689
BstNSI RCATGY 1 cut(s) 860
BstSCI CCNGG 6 cut(s) 78, 309, 371, 372, 687, 741
BstUI CGCG 1 cut(s) 20
BstV2I GAAGAC 3 cut(s) 96, 440, 458
BstX2I RGATCY 1 cut(s) 171
BstYI RGATCY 1 cut(s) 171
Bsu15I ATCGAT 1 cut(s) 399
BsuI GTATCC 1 cut(s) 769
BsuRI GGCC 6 cut(s) 8, 123, 938, 1136, 1151, 1257
BsuTUI ATCGAT 1 cut(s) 399
BtgI CCRYGG 1 cut(s) 575
BtsCI GGATG 1 cut(s) 1258
BtsI GCAGTG 2 cut(s) 605, 654
BtsIMutI CAGTG 5 cut(s) 105, 605, 622, 654, 939
BtuMI TCGCGA 1 cut(s) 20
Cac8I GCNNGC 4 cut(s) 18, 477, 582, 917
CfoI GCGC 2 cut(s) 65, 871
Cfr13I GGNCC 4 cut(s) 122, 739, 1150, 1255
Cfr9I CCCGGG 1 cut(s) 372
ClaI ATCGAT 1 cut(s) 399
CseI GACGC 1 cut(s) 77
Csp6I GTAC 3 cut(s) 587, 1197, 1224
CviAII CATG 9 cut(s) 557, 564, 576, 635, 713, 791, 857, 956, 1088
CviQI GTAC 3 cut(s) 587, 1197, 1224
DdeI CTNAG 6 cut(s) 161, 168, 387, 471, 492, 705
DpnI GATC 7 cut(s) 115, 146, 156, 173, 398, 885, 930
DpnII GATC 7 cut(s) 113, 144, 154, 171, 396, 883, 928
DraI TTTAAA 1 cut(s) 414
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 936
Eam1104I CTCTTC 2 cut(s) 234, 1076
EarI CTCTTC 2 cut(s) 234, 1076
EciI GGCGGA 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 575
Eco24I GRGCYC 1 cut(s) 711
Eco47I GGWCC 1 cut(s) 739
Eco47III AGCGCT 1 cut(s) 64
Eco57I CTGAAG 3 cut(s) 57, 161, 1100
Eco88I CYCGRG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 739
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 309, 687
EcoT14I CCWWGG 1 cut(s) 575
EcoT38I GRGCYC 1 cut(s) 711
ErhI CCWWGG 1 cut(s) 575
FaeI CATG 9 cut(s) 560, 567, 579, 638, 716, 794, 860, 959, 1091
FaqI GGGAC 3 cut(s) 62, 725, 1075
FatI CATG 9 cut(s) 556, 563, 575, 634, 712, 790, 856, 955, 1087
Fnu4HI GCNGC 2 cut(s) 6, 1134
FokI GGATG 1 cut(s) 1245
FriOI GRGCYC 1 cut(s) 711
Fsp4HI GCNGC 2 cut(s) 6, 1134
FspBI CTAG 3 cut(s) 182, 614, 848
GlaI GCGC 2 cut(s) 64, 870
GluI GCNGC 2 cut(s) 6, 1134
GsuI CTGGAG 2 cut(s) 293, 695
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 6 cut(s) 8, 123, 938, 1136, 1151, 1257
HapII CCGG 3 cut(s) 80, 373, 743
HgaI GACGC 1 cut(s) 77
HhaI GCGC 2 cut(s) 65, 871
Hin1II CATG 9 cut(s) 560, 567, 579, 638, 716, 794, 860, 959, 1091
Hin6I GCGC 2 cut(s) 63, 869
HinP1I GCGC 2 cut(s) 63, 869
HinfI GANTC 4 cut(s) 22, 128, 302, 568
HpaII CCGG 3 cut(s) 80, 373, 743
HphI GGTGA 2 cut(s) 209, 781
Hpy188I TCNGA 6 cut(s) 113, 118, 141, 271, 666, 1175
Hpy188III TCNNGA 6 cut(s) 19, 260, 614, 674, 896, 1262
Hpy99I CGWCG 1 cut(s) 1242
HpyAV CCTTC 4 cut(s) 238, 661, 766, 991
HpyCH4III ACNGT 3 cut(s) 781, 943, 1066
HpyCH4IV ACGT 2 cut(s) 346, 1240
HpyCH4V TGCA 6 cut(s) 289, 556, 598, 659, 809, 1034
HpyF10VI GCNNNNNNNGC 7 cut(s) 103, 383, 944, 1007, 1084, 1139, 1148
HpyF3I CTNAG 6 cut(s) 161, 168, 387, 471, 492, 705
HpySE526I ACGT 2 cut(s) 346, 1240
Hsp92II CATG 9 cut(s) 560, 567, 579, 638, 716, 794, 860, 959, 1091
HspAI GCGC 2 cut(s) 63, 869
KflI GGGWCCC 1 cut(s) 739
KpnI GGTACC 1 cut(s) 1227
Kzo9I GATC 7 cut(s) 113, 144, 154, 171, 396, 883, 928
LmnI GCTCC 4 cut(s) 56, 111, 560, 856
MaeI CTAG 3 cut(s) 182, 614, 848
MaeII ACGT 2 cut(s) 346, 1240
MalI GATC 7 cut(s) 115, 146, 156, 173, 398, 885, 930
MbiI CCGCTC 1 cut(s) 137
MboI GATC 7 cut(s) 113, 144, 154, 171, 396, 883, 928
MflI RGATCY 1 cut(s) 171
MhlI GDGCHC 2 cut(s) 481, 711
MlsI TGGCCA 1 cut(s) 938
MluCI AATT 6 cut(s) 45, 185, 332, 660, 1164, 1176
MluNI TGGCCA 1 cut(s) 938
MlyI GAGTC 1 cut(s) 311
MmeI TCCRAC 2 cut(s) 132, 1101
Mox20I TGGCCA 1 cut(s) 938
MscI TGGCCA 1 cut(s) 938
MseI TTAA 9 cut(s) 207, 315, 324, 413, 483, 506, 545, 725, 1248
Msp20I TGGCCA 1 cut(s) 938
MspI CCGG 3 cut(s) 80, 373, 743
MspR9I CCNGG 6 cut(s) 80, 311, 373, 374, 689, 743
MvaI CCWGG 2 cut(s) 311, 689
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 7 cut(s) 103, 383, 944, 1007, 1084, 1139, 1148
NciI CCSGG 4 cut(s) 80, 373, 374, 743
NcoI CCATGG 1 cut(s) 575
NdeII GATC 7 cut(s) 113, 144, 154, 171, 396, 883, 928
NlaIII CATG 9 cut(s) 560, 567, 579, 638, 716, 794, 860, 959, 1091
NlaIV GGNNCC 4 cut(s) 740, 741, 1225, 1256
NmeAIII GCCGAG 1 cut(s) 1118
NruI TCGCGA 1 cut(s) 20
NspI RCATGY 1 cut(s) 860
PciI ACATGT 1 cut(s) 856
PfeI GAWTC 3 cut(s) 22, 128, 568
PkrI GCNGC 2 cut(s) 7, 1135
Ple19I CGATCG 1 cut(s) 116
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
PpuMI RGGWCCY 1 cut(s) 739
PscI ACATGT 1 cut(s) 856
Psp5II RGGWCCY 1 cut(s) 739
Psp6I CCWGG 2 cut(s) 309, 687
PspGI CCWGG 2 cut(s) 309, 687
PspN4I GGNNCC 4 cut(s) 740, 741, 1225, 1256
PspPI GGNCC 4 cut(s) 122, 739, 1150, 1255
PspPPI RGGWCCY 1 cut(s) 739
PsuI RGATCY 1 cut(s) 171
PvuI CGATCG 1 cut(s) 116
RruI TCGCGA 1 cut(s) 20
RsaI GTAC 3 cut(s) 588, 1198, 1225
RsaNI GTAC 3 cut(s) 587, 1197, 1224
SaqAI TTAA 9 cut(s) 207, 315, 324, 413, 483, 506, 545, 725, 1248
SatI GCNGC 2 cut(s) 6, 1134
Sau3AI GATC 7 cut(s) 113, 144, 154, 171, 396, 883, 928
Sau96I GGNCC 4 cut(s) 122, 739, 1150, 1255
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 6 cut(s) 80, 311, 373, 374, 689, 743
SduI GDGCHC 2 cut(s) 481, 711
SinI GGWCC 1 cut(s) 739
SmaI CCCGGG 1 cut(s) 374
SrfI GCCCGGGC 1 cut(s) 374
Sse9I AATT 6 cut(s) 45, 185, 332, 660, 1164, 1176
SsiI CCGC 4 cut(s) 5, 83, 135, 1133
SspMI CTAG 3 cut(s) 182, 614, 848
StyD4I CCNGG 6 cut(s) 78, 309, 371, 372, 687, 741
StyI CCWWGG 1 cut(s) 575
TaaI ACNGT 3 cut(s) 781, 943, 1066
TaiI ACGT 2 cut(s) 349, 1243
TaqI TCGA 7 cut(s) 147, 189, 300, 399, 960, 970, 1219
TasI AATT 6 cut(s) 45, 185, 332, 660, 1164, 1176
TatI WGTACW 1 cut(s) 586
TauI GCSGC 2 cut(s) 8, 1136
TfiI GAWTC 3 cut(s) 22, 128, 568
Tru1I TTAA 9 cut(s) 207, 315, 324, 413, 483, 506, 545, 725, 1248
Tru9I TTAA 9 cut(s) 207, 315, 324, 413, 483, 506, 545, 725, 1248
TscAI CASTG 5 cut(s) 105, 605, 629, 654, 946
TspDTI ATGAA 4 cut(s) 333, 396, 623, 1104
TspGWI ACGGA 1 cut(s) 452
TspMI CCCGGG 1 cut(s) 372
TspRI CASTG 5 cut(s) 105, 605, 629, 654, 946
VpaK11BI GGWCC 1 cut(s) 739
XapI RAATTY 2 cut(s) 185, 1176
XbaI TCTAGA 1 cut(s) 613
XceI RCATGY 1 cut(s) 860
XmaI CCCGGG 1 cut(s) 372
XspI CTAG 3 cut(s) 182, 614, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.