FvH4_2g36360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007104
Physical Location & Seq
Reverse (-)
26621663 .. 26626997
5335 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36360.t1

Sequence Viewer

Length: 1332 bp
ATGGCGTCCTCAAAGCTCTTGATTCTCTCAGTCTTTCTAGCCCTAATTTTCACGCAAATTAGGGCCGACGCTTCAGTCGGAGAAGACGAGCCACTGGAAGTAGTCCGATCGGACGGCCCTGATTCCTCCGCTCTGAAGATCGAGTTGGATCAGCTCAAGTCGAAGATCCACTCGCTTGAATCTGAAGTAGATGAGAAAGCCAAGCAACTGCAGAGTAAGGATGAGATAGTAGCGCAGAAGAGTAAAATCATTAAAGTGAACTCGGATGTCATCACGGCTCTGCAGAAAGAAGTTGATTCTCTCCAGAAAAAAGGGTCGCGGGATGCTGAGGTTGACAAGGCTCATGCACGCGCTGGTGAATTAGAGAAGCAGGTTGACCAACTGGCAAAGGAATTGGAAGCACAACATAAAGAGAAAGAGGCTTTGGAAGCCCGAGCCAATCAAGCGGAGAAGAAGATTGATGTGTTGCTTTTAAAGATAGATAAGATTGAGAAGACTAATGAGGAACAAAAGACCAAAATTCGAAAGACTGAGCGTGCTCTTAAAGTTGCTGAGGAAGAAATGTTGAAGGCAAAGTTTGAGGCTACTTTGAAAGCAAAAGCATTAACAGAGGTTCATGGTGCATGGCTTCCACATTGGCTTGCTGTTCATTTTGGCTATTGTCAGGACTTTGTAGAGAAACACTGGAATGAGCATGGAAAACCTGCTGCCGAGGTGGTGATTCAGAAGGCCCTGGAGAAAAAAGCTCAAGCTGGGAAGTGGGCTGAACCCCATGTGGAAGCAGTTAAAACTAAATGGGTCCCAGCAGTCAAGGAACAGTGGTCAGTTTTGAAAGAATCTGTTGAACCTCATGTGCAATCACTAAAAACAAAGTCTATTGAAGCTTACAAGACATCAAAGAGTACATTAGCTCCGCATGTTATAAAAGCACAAGAATGTGTTGATCCCTACTTTCAGGAGGCAAAAAGGTTCAGCAAGCCATATGTTGATCAGGTAGCTACTGTTGCGAAACCTCATGTTGAAAAAGTTAGAGTGGTGTTAAAGCCATATACAAAGCAGGCAGTTCAGGCCTATGAGAAGTTTCTCAAATCCGCTTCAACATATCATCATCAGGTTCAAACCATTGTACGAGAGAAACTGAAAAAGCATGAACTGACAAGACCTCTTGCTACAAAAGAGTTGGAGTGGTTTGCGGCCTCTGCTTTGTTGGCGCTTCCGATCTTAATTCTGTTCAGAGTTTTATCTTCACTCTTCTGTACAAAGTCAAAGAAACCTGTAAGGAGTACCCACCATTCCCGTCGCAAGGCCAAAAGGGGTCATCCAGACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

444

Amino Acids

50.2

Weight (kDa)

9.38

Isoelectric Point (pI)

28.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Apolipoprotein PF01442 275 - 379 9.3e-06 Apolipoprotein A1/A4/E domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 923
AasI GACNNNNNNGTC 1 cut(s) 74
Acc36I ACCTGC 2 cut(s) 361, 712
AccBSI CCGCTC 1 cut(s) 131
AccII CGCG 2 cut(s) 319, 351
AciI CCGC 6 cut(s) 129, 319, 446, 914, 1092, 1193
AclWI GGATC 3 cut(s) 156, 160, 938
AcsI RAATTY 1 cut(s) 519
AcuI CTGAAG 3 cut(s) 57, 155, 204
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 904, 1128, 1258, 1285
AfiI CCNNNNNNNGG 1 cut(s) 1303
AgsI TTSAA 9 cut(s) 179, 568, 592, 832, 845, 881, 1022, 1098, 1118
AjnI CCWGG 1 cut(s) 732
AjuI GAANNNNNNNTTGG 4 cut(s) 128, 160, 407, 439
AluBI AGCT 7 cut(s) 16, 154, 746, 752, 884, 911, 998
AluI AGCT 7 cut(s) 16, 154, 746, 752, 884, 911, 998
Alw21I GWGCWC 1 cut(s) 541
AlwI GGATC 3 cut(s) 156, 160, 938
Ama87I CYCGRG 1 cut(s) 432
AoxI GGCC 6 cut(s) 63, 115, 729, 1068, 1194, 1305
ApeKI GCWGC 1 cut(s) 707
ApoI RAATTY 1 cut(s) 519
AspLEI GCGC 3 cut(s) 235, 353, 1213
AspS9I GGNCC 4 cut(s) 63, 116, 730, 799
AsuHPI GGTGA 2 cut(s) 368, 730
AsuII TTCGAA 1 cut(s) 523
AvaI CYCGRG 1 cut(s) 432
AvaII GGWCC 1 cut(s) 799
BbsI GAAGAC 2 cut(s) 90, 500
Bbv12I GWGCWC 1 cut(s) 541
BbvCI CCTCAGC 2 cut(s) 327, 552
BbvI GCAGC 1 cut(s) 694
BceAI ACGGC 2 cut(s) 130, 291
BciT130I CCWGG 1 cut(s) 734
BclI TGATCA 1 cut(s) 988
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 2 cut(s) 209, 281
BfoI RGCGCY 1 cut(s) 1214
BfuAI ACCTGC 2 cut(s) 361, 712
BisI GCNGC 2 cut(s) 708, 1194
BlsI GCNGC 2 cut(s) 709, 1195
Bme1390I CCNGG 1 cut(s) 734
Bme18I GGWCC 1 cut(s) 799
BmeT110I CYCGRG 1 cut(s) 432
BmgT120I GGNCC 4 cut(s) 63, 116, 730, 799
BmiI GGNNCC 2 cut(s) 800, 801
BmrFI CCNGG 1 cut(s) 734
BmsI GCATC 1 cut(s) 313
BpiI GAAGAC 2 cut(s) 90, 500
BpmI CTGGAG 2 cut(s) 287, 755
Bpu10I CCTNAGC 2 cut(s) 327, 552
Bpu14I TTCGAA 1 cut(s) 523
BpuEI CTTGAG 2 cut(s) 140, 732
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 711, 732
BsaXI ACNNNNNCTCC 2 cut(s) 895, 925
Bsc4I CCNNNNNNNGG 1 cut(s) 1303
Bse1I ACTGG 3 cut(s) 99, 387, 689
BseBI CCWGG 1 cut(s) 734
BseDI CCNNGG 2 cut(s) 711, 732
BseGI GGATG 4 cut(s) 226, 271, 328, 1318
BseLI CCNNNNNNNGG 1 cut(s) 1303
BseMII CTCAG 4 cut(s) 42, 318, 522, 543
BseNI ACTGG 3 cut(s) 99, 387, 689
BseXI GCAGC 1 cut(s) 694
BseYI CCCAGC 2 cut(s) 752, 802
Bsh1236I CGCG 2 cut(s) 319, 351
Bsh1285I CGRYCG 1 cut(s) 110
BshFI GGCC 6 cut(s) 65, 117, 731, 1070, 1196, 1307
BsiEI CGRYCG 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 541
BsiHKCI CYCGRG 1 cut(s) 432
BslFI GGGAC 1 cut(s) 785
BslI CCNNNNNNNGG 1 cut(s) 1303
BsmFI GGGAC 1 cut(s) 785
BsnI GGCC 6 cut(s) 65, 117, 731, 1070, 1196, 1307
BsoBI CYCGRG 1 cut(s) 432
Bsp119I TTCGAA 1 cut(s) 523
Bsp1286I GDGCHC 1 cut(s) 541
Bsp1407I TGTACA 1 cut(s) 1256
Bsp143I GATC 7 cut(s) 107, 138, 148, 165, 943, 988, 1218
BspACI CCGC 6 cut(s) 129, 319, 446, 914, 1092, 1193
BspANI GGCC 6 cut(s) 65, 117, 731, 1070, 1196, 1307
BspCNI CTCAG 4 cut(s) 41, 319, 523, 544
BspFNI CGCG 2 cut(s) 319, 351
BspLI GGNNCC 2 cut(s) 800, 801
BspMAI CTGCAG 2 cut(s) 213, 285
BspMI ACCTGC 2 cut(s) 361, 712
BspPI GGATC 3 cut(s) 156, 160, 938
BspT104I TTCGAA 1 cut(s) 523
BsrBI CCGCTC 1 cut(s) 131
BsrGI TGTACA 1 cut(s) 1256
BsrI ACTGG 3 cut(s) 99, 387, 689
BssECI CCNNGG 2 cut(s) 711, 732
BssMI GATC 7 cut(s) 107, 138, 148, 165, 943, 988, 1218
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 734
Bst4CI ACNGT 2 cut(s) 819, 1003
Bst6I CTCTTC 2 cut(s) 233, 1256
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 1256
BstBI TTCGAA 1 cut(s) 523
BstC8I GCNNGC 5 cut(s) 349, 537, 642, 977, 1059
BstDEI CTNAG 4 cut(s) 28, 327, 531, 552
BstF5I GGATG 4 cut(s) 226, 271, 328, 1318
BstFNI CGCG 2 cut(s) 319, 351
BstH2I RGCGCY 1 cut(s) 1214
BstHHI GCGC 3 cut(s) 235, 353, 1213
BstKTI GATC 7 cut(s) 110, 141, 151, 168, 946, 991, 1221
BstMBI GATC 7 cut(s) 107, 138, 148, 165, 943, 988, 1218
BstMCI CGRYCG 1 cut(s) 110
BstMWI GCNNNNNNNGC 6 cut(s) 428, 443, 1004, 1067, 1199, 1208
BstNI CCWGG 1 cut(s) 734
BstNSI RCATGY 1 cut(s) 920
BstSCI CCNGG 1 cut(s) 732
BstSFI CTRYAG 2 cut(s) 209, 281
BstUI CGCG 2 cut(s) 319, 351
BstV1I GCAGC 1 cut(s) 694
BstV2I GAAGAC 2 cut(s) 90, 500
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
BsuRI GGCC 6 cut(s) 65, 117, 731, 1070, 1196, 1307
BtsCI GGATG 4 cut(s) 226, 271, 328, 1318
BtsIMutI CAGTG 3 cut(s) 92, 682, 824
BveI ACCTGC 2 cut(s) 361, 712
Cac8I GCNNGC 5 cut(s) 349, 537, 642, 977, 1059
CfoI GCGC 3 cut(s) 235, 353, 1213
Cfr13I GGNCC 4 cut(s) 63, 116, 730, 799
CseI GACGC 1 cut(s) 77
Csp6I GTAC 4 cut(s) 903, 1127, 1257, 1284
CviAII CATG 9 cut(s) 344, 617, 624, 695, 773, 851, 917, 1016, 1148
CviQI GTAC 4 cut(s) 903, 1127, 1257, 1284
DdeI CTNAG 4 cut(s) 28, 327, 531, 552
DpnI GATC 7 cut(s) 109, 140, 150, 167, 945, 990, 1220
DpnII GATC 7 cut(s) 107, 138, 148, 165, 943, 988, 1218
DraI TTTAAA 1 cut(s) 474
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
Eam1104I CTCTTC 2 cut(s) 233, 1256
EarI CTCTTC 2 cut(s) 233, 1256
Eco147I AGGCCT 1 cut(s) 1070
Eco47I GGWCC 1 cut(s) 799
Eco57I CTGAAG 3 cut(s) 57, 155, 204
Eco88I CYCGRG 1 cut(s) 432
EcoO109I RGGNCCY 2 cut(s) 730, 799
EcoRII CCWGG 1 cut(s) 732
FaeI CATG 9 cut(s) 347, 620, 627, 698, 776, 854, 920, 1019, 1151
FaqI GGGAC 1 cut(s) 785
FatI CATG 9 cut(s) 343, 616, 623, 694, 772, 850, 916, 1015, 1147
FauI CCCGC 1 cut(s) 312
FauNDI CATATG 1 cut(s) 982
FbaI TGATCA 1 cut(s) 988
Fnu4HI GCNGC 2 cut(s) 708, 1194
FokI GGATG 4 cut(s) 233, 278, 335, 1305
Fsp4HI GCNGC 2 cut(s) 708, 1194
FspBI CTAG 1 cut(s) 38
GlaI GCGC 3 cut(s) 234, 352, 1212
GluI GCNGC 2 cut(s) 708, 1194
GsaI CCCAGC 2 cut(s) 756, 806
GsuI CTGGAG 2 cut(s) 287, 755
HaeII RGCGCY 1 cut(s) 1214
HaeIII GGCC 6 cut(s) 65, 117, 731, 1070, 1196, 1307
HgaI GACGC 1 cut(s) 77
HhaI GCGC 3 cut(s) 235, 353, 1213
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 9 cut(s) 347, 620, 627, 698, 776, 854, 920, 1019, 1151
Hin6I GCGC 3 cut(s) 233, 351, 1211
HinP1I GCGC 3 cut(s) 233, 351, 1211
HincII GTYRAC 2 cut(s) 334, 376
HindII GTYRAC 2 cut(s) 334, 376
HindIII AAGCTT 1 cut(s) 882
HinfI GANTC 6 cut(s) 22, 122, 179, 296, 721, 836
HphI GGTGA 2 cut(s) 368, 730
Hpy166II GTNNAC 3 cut(s) 259, 334, 376
Hpy188I TCNGA 9 cut(s) 80, 107, 112, 135, 184, 265, 726, 1218, 1235
Hpy188III TCNNGA 5 cut(s) 19, 304, 665, 956, 1322
Hpy8I GTNNAC 3 cut(s) 259, 334, 376
Hpy99I CGWCG 2 cut(s) 71, 1302
HpyAV CCTTC 2 cut(s) 562, 721
HpyCH4III ACNGT 2 cut(s) 819, 1003
HpyCH4V TGCA 5 cut(s) 211, 283, 347, 623, 856
HpyF10VI GCNNNNNNNGC 6 cut(s) 428, 443, 1004, 1067, 1199, 1208
HpyF3I CTNAG 4 cut(s) 28, 327, 531, 552
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 9 cut(s) 347, 620, 627, 698, 776, 854, 920, 1019, 1151
HspAI GCGC 3 cut(s) 233, 351, 1211
KflI GGGWCCC 1 cut(s) 799
Ksp22I TGATCA 1 cut(s) 988
Kzo9I GATC 7 cut(s) 107, 138, 148, 165, 943, 988, 1218
LmnI GCTCC 1 cut(s) 916
Lsp1109I GCAGC 1 cut(s) 694
LweI GCATC 1 cut(s) 313
MaeI CTAG 1 cut(s) 38
MalI GATC 7 cut(s) 109, 140, 150, 167, 945, 990, 1220
MbiI CCGCTC 1 cut(s) 131
MboI GATC 7 cut(s) 107, 138, 148, 165, 943, 988, 1218
MflI RGATCY 1 cut(s) 165
MhlI GDGCHC 1 cut(s) 541
MluCI AATT 6 cut(s) 45, 57, 359, 392, 519, 1224
MmeI TCCRAC 3 cut(s) 58, 126, 1161
MseI TTAA 7 cut(s) 252, 473, 543, 605, 786, 1040, 1223
MslI CAYNNNNRTG 3 cut(s) 254, 687, 934
MspR9I CCNGG 1 cut(s) 734
MvaI CCWGG 1 cut(s) 734
MvnI CGCG 2 cut(s) 319, 351
MwoI GCNNNNNNNGC 6 cut(s) 428, 443, 1004, 1067, 1199, 1208
NdeI CATATG 1 cut(s) 982
NdeII GATC 7 cut(s) 107, 138, 148, 165, 943, 988, 1218
NlaIII CATG 9 cut(s) 347, 620, 627, 698, 776, 854, 920, 1019, 1151
NlaIV GGNNCC 2 cut(s) 800, 801
NmeAIII GCCGAG 1 cut(s) 736
NspI RCATGY 1 cut(s) 920
NspV TTCGAA 1 cut(s) 523
PceI AGGCCT 1 cut(s) 1070
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 6 cut(s) 22, 122, 179, 296, 721, 836
PkrI GCNGC 2 cut(s) 709, 1195
Ple19I CGATCG 1 cut(s) 110
PpuMI RGGWCCY 1 cut(s) 799
PsiI TTATAA 1 cut(s) 923
Psp5II RGGWCCY 1 cut(s) 799
Psp6I CCWGG 1 cut(s) 732
PspFI CCCAGC 2 cut(s) 752, 802
PspGI CCWGG 1 cut(s) 732
PspN4I GGNNCC 2 cut(s) 800, 801
PspPI GGNCC 4 cut(s) 63, 116, 730, 799
PspPPI RGGWCCY 1 cut(s) 799
PstI CTGCAG 2 cut(s) 213, 285
PsuI RGATCY 1 cut(s) 165
PvuI CGATCG 1 cut(s) 110
RsaI GTAC 4 cut(s) 904, 1128, 1258, 1285
RsaNI GTAC 4 cut(s) 903, 1127, 1257, 1284
RseI CAYNNNNRTG 3 cut(s) 254, 687, 934
SaqAI TTAA 7 cut(s) 252, 473, 543, 605, 786, 1040, 1223
SatI GCNGC 2 cut(s) 708, 1194
Sau3AI GATC 7 cut(s) 107, 138, 148, 165, 943, 988, 1218
Sau96I GGNCC 4 cut(s) 63, 116, 730, 799
ScrFI CCNGG 1 cut(s) 734
SduI GDGCHC 1 cut(s) 541
SfaNI GCATC 1 cut(s) 313
SfcI CTRYAG 2 cut(s) 209, 281
SfuI TTCGAA 1 cut(s) 523
SinI GGWCC 1 cut(s) 799
SmiMI CAYNNNNRTG 3 cut(s) 254, 687, 934
SmlI CTYRAG 2 cut(s) 155, 747
SmoI CTYRAG 2 cut(s) 155, 747
Sse9I AATT 6 cut(s) 45, 57, 359, 392, 519, 1224
SseBI AGGCCT 1 cut(s) 1070
SsiI CCGC 6 cut(s) 129, 319, 446, 914, 1092, 1193
SspMI CTAG 1 cut(s) 38
StuI AGGCCT 1 cut(s) 1070
StyD4I CCNGG 1 cut(s) 732
TaaI ACNGT 2 cut(s) 819, 1003
TaqI TCGA 3 cut(s) 141, 161, 523
TasI AATT 6 cut(s) 45, 57, 359, 392, 519, 1224
TatI WGTACW 2 cut(s) 902, 1256
TauI GCSGC 1 cut(s) 1196
TfiI GAWTC 6 cut(s) 22, 122, 179, 296, 721, 836
Tru1I TTAA 7 cut(s) 252, 473, 543, 605, 786, 1040, 1223
Tru9I TTAA 7 cut(s) 252, 473, 543, 605, 786, 1040, 1223
TscAI CASTG 3 cut(s) 99, 689, 824
TseI GCWGC 1 cut(s) 707
TspDTI ATGAA 3 cut(s) 605, 638, 1164
TspRI CASTG 3 cut(s) 99, 689, 824
VpaK11BI GGWCC 1 cut(s) 799
XapI RAATTY 1 cut(s) 519
XceI RCATGY 1 cut(s) 920
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.