RchiOBHm_Chr6g0305521

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007104
Physical Location & Seq
Forward (+)
64499531 .. 64504709
5179 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27457

Sequence Viewer

Length: 1338 bp
ATGGCGTCCTCAAAGCTCTTGATTCTCTCGGTCTTTCTGGCCCTAGTTTTCTCGCAAATTAGGGGTGAGGCTTCAGTCAAAGAAGATGAGCCACTGGAAGTAGTCCGATCGGACGGCCCTGATTCGTCCGCGCTGAAGATCGAGTTGGATCAACTCAGGTCGAAGATCCACTCACTCGAATTTGAACTGGAGGAGAAAGTTAAGCAACTTCACAGTAAGGATGAGATAGTAGAACAGAAGAATAAAATCATTCAAGTGAACTCGGATGTCATCATGGCTCTGCAGAAAGAAGTTGATTCTCTCCAGAAAAAAGGGTCGCTGGATGTTGAGAAGCAGGTTGGCGAGGCTCATGCACGAGCTGGTGAATTAGAGAAGCAGGTTGACAAACTTAAAAAGGAATTGGAAGCACAACATAAGGAGAAAGAGGCCTTGGAAGCCCGTGCCAATCAGGCTGAGAAGAAGATTGACGTTTTGATCTTGAAAATAGATAAGATCGAGAAGACTAATGATGAACAGAAGACCAAAATTCGAAATACTGAGCGTGCTCTTAAAGTTGCTGAGGAAGAAATGTTGAGGGCAAAGTTTGAGGCTACTTCTAAAGCAAAAGCGTTAACAGAGGTTCATGGTGCATGGCTTCCACATTGGCTTGCTGTTCATTTCGGCTATTGTCAGTCCTTTGTAGAGAAATACTGGAATGAGCATGGAAAACCTGCTGCAGAGGTGGTGATTCAGAAGGCCCTTGAGAAAAAAGCTCAAGCTGGAAAGTGGGCTGAACCCCATGTGGAAGCAGTTAAAACTAAATGGATCCCGGCAGTCAAGGAACAGTGGTCAGTGGCGAAAGATTCTGTTGAACCTCATGTGCAATCACTAAGAACAAAGTCTATTGAAGCTTATAAGACATCAAAGAGTACATTAGCTCCCCATGCTATAAAAGCACAAGAGTTTGTTGATCCTTACTTTCAGGAGGCAAAAAAGTTCAGCAAGCCATATGTTGATCAGGTAGCCACTGTTGCAAAACCTCATGTTGAAAAAGTTAGAGTGGTGTTGAAGCCCTATACAAAGCAGGTTGTTCAGGCCTACGAGAAGTTTCTCAAATCCGCTTCAATATATCATCATCAGGTTCAAACTACCGTACGAGATACACTGAAAAAGCATGAACTGACAAGACCTCTTGCTACGAAAGAGTTGGAGTGGTTTGCGGCCTCTGCTTTGTTGGCACTTCCCATCTTAATTCTGTTCAGAGTTTTATCCTCAATTTTCTGTACAAAGTCAACAAAACCTGTACGGAGTACCCACCATTCACGTCGCAAGGCCAAAAGGGGTCATCCAGACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

445

Amino Acids

50.66

Weight (kDa)

9.35

Isoelectric Point (pI)

30.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Apolipoprotein PF01442 278 - 382 3.8e-06 Apolipoprotein A1/A4/E domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 894
Acc36I ACCTGC 4 cut(s) 325, 367, 718, 1054
AccII CGCG 1 cut(s) 131
AciI CCGC 3 cut(s) 129, 1098, 1199
AclWI GGATC 5 cut(s) 156, 160, 799, 812, 944
AcsI RAATTY 2 cut(s) 179, 525
AcuI CTGAAG 2 cut(s) 57, 155
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 910, 1134, 1264, 1284, 1291
AgsI TTSAA 9 cut(s) 185, 254, 481, 851, 887, 1028, 1048, 1104, 1124
AjiI CACGTC 1 cut(s) 1304
AjuI GAANNNNNNNTTGG 4 cut(s) 128, 160, 413, 445
AluBI AGCT 6 cut(s) 16, 359, 752, 758, 890, 917
AluI AGCT 6 cut(s) 16, 359, 752, 758, 890, 917
Alw21I GWGCWC 1 cut(s) 547
AlwI GGATC 5 cut(s) 156, 160, 799, 812, 944
AoxI GGCC 7 cut(s) 39, 115, 426, 735, 1074, 1200, 1311
ApeKI GCWGC 1 cut(s) 713
ApoI RAATTY 2 cut(s) 179, 525
AspLEI GCGC 1 cut(s) 133
AspS9I GGNCC 3 cut(s) 40, 116, 736
AsuC2I CCSGG 1 cut(s) 809
AsuHPI GGTGA 3 cut(s) 77, 374, 736
AsuII TTCGAA 1 cut(s) 529
BamHI GGATCC 1 cut(s) 804
BauI CACGAG 1 cut(s) 354
BbsI GAAGAC 2 cut(s) 506, 524
Bbv12I GWGCWC 1 cut(s) 547
BbvCI CCTCAGC 1 cut(s) 558
BbvI GCAGC 1 cut(s) 700
BccI CCATC 1 cut(s) 1232
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BclI TGATCA 1 cut(s) 994
BcnI CCSGG 1 cut(s) 809
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 2 cut(s) 281, 714
BfuAI ACCTGC 4 cut(s) 325, 367, 718, 1054
BglI GCCNNNNNGGC 1 cut(s) 449
BisI GCNGC 2 cut(s) 714, 1200
BlsI GCNGC 2 cut(s) 715, 1201
Bme1390I CCNGG 1 cut(s) 809
BmgBI CACGTC 1 cut(s) 1304
BmgT120I GGNCC 3 cut(s) 40, 116, 736
BmiI GGNNCC 1 cut(s) 806
BmrFI CCNGG 1 cut(s) 809
BpiI GAAGAC 2 cut(s) 506, 524
BpmI CTGGAG 2 cut(s) 209, 287
Bpu10I CCTNAGC 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 529
BpuEI CTTGAG 2 cut(s) 738, 761
BpuMI CCSGG 1 cut(s) 809
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 429
BsaXI ACNNNNNCTCC 2 cut(s) 901, 931
Bse1I ACTGG 3 cut(s) 99, 192, 695
BseDI CCNNGG 1 cut(s) 429
BseGI GGATG 4 cut(s) 226, 271, 328, 1324
BseMII CTCAG 4 cut(s) 169, 444, 528, 549
BseNI ACTGG 3 cut(s) 99, 192, 695
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 700
Bsh1236I CGCG 1 cut(s) 131
Bsh1285I CGRYCG 1 cut(s) 110
BshFI GGCC 7 cut(s) 41, 117, 428, 737, 1076, 1202, 1313
BsiEI CGRYCG 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 547
BsiSI CCGG 1 cut(s) 809
BsiWI CGTACG 1 cut(s) 1132
BsnI GGCC 7 cut(s) 41, 117, 428, 737, 1076, 1202, 1313
Bsp119I TTCGAA 1 cut(s) 529
Bsp1286I GDGCHC 1 cut(s) 547
Bsp1407I TGTACA 1 cut(s) 1262
Bsp143I GATC 9 cut(s) 107, 138, 148, 165, 474, 492, 804, 949, 994
BspACI CCGC 3 cut(s) 129, 1098, 1199
BspANI GGCC 7 cut(s) 41, 117, 428, 737, 1076, 1202, 1313
BspCNI CTCAG 4 cut(s) 168, 445, 529, 550
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 1 cut(s) 806
BspMAI CTGCAG 2 cut(s) 285, 718
BspMI ACCTGC 4 cut(s) 325, 367, 718, 1054
BspPI GGATC 5 cut(s) 156, 160, 799, 812, 944
BspT104I TTCGAA 1 cut(s) 529
BsrGI TGTACA 1 cut(s) 1262
BsrI ACTGG 3 cut(s) 99, 192, 695
BssECI CCNNGG 1 cut(s) 429
BssMI GATC 9 cut(s) 107, 138, 148, 165, 474, 492, 804, 949, 994
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 1 cut(s) 429
Bst2BI CACGAG 1 cut(s) 354
Bst4CI ACNGT 4 cut(s) 215, 825, 1009, 1132
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 1262
BstBI TTCGAA 1 cut(s) 529
BstC8I GCNNGC 3 cut(s) 543, 648, 983
BstDEI CTNAG 5 cut(s) 155, 453, 537, 558, 869
BstF5I GGATG 4 cut(s) 226, 271, 328, 1324
BstFNI CGCG 1 cut(s) 131
BstHHI GCGC 1 cut(s) 133
BstKTI GATC 9 cut(s) 110, 141, 151, 168, 477, 495, 807, 952, 997
BstMBI GATC 9 cut(s) 107, 138, 148, 165, 474, 492, 804, 949, 994
BstMCI CGRYCG 1 cut(s) 110
BstMWI GCNNNNNNNGC 7 cut(s) 434, 449, 923, 932, 1010, 1205, 1214
BstSCI CCNGG 1 cut(s) 807
BstSFI CTRYAG 2 cut(s) 281, 714
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 1 cut(s) 700
BstV2I GAAGAC 2 cut(s) 506, 524
BstX2I RGATCY 2 cut(s) 165, 804
BstYI RGATCY 2 cut(s) 165, 804
BsuRI GGCC 7 cut(s) 41, 117, 428, 737, 1076, 1202, 1313
BtrI CACGTC 1 cut(s) 1304
BtsCI GGATG 4 cut(s) 226, 271, 328, 1324
BtsIMutI CAGTG 5 cut(s) 92, 830, 837, 1005, 1142
BveI ACCTGC 4 cut(s) 325, 367, 718, 1054
Cac8I GCNNGC 3 cut(s) 543, 648, 983
CfoI GCGC 1 cut(s) 133
Cfr13I GGNCC 3 cut(s) 40, 116, 736
Csp6I GTAC 5 cut(s) 909, 1133, 1263, 1283, 1290
CviQI GTAC 5 cut(s) 909, 1133, 1263, 1283, 1290
DdeI CTNAG 5 cut(s) 155, 453, 537, 558, 869
DpnI GATC 9 cut(s) 109, 140, 150, 167, 476, 494, 806, 951, 996
DpnII GATC 9 cut(s) 107, 138, 148, 165, 474, 492, 804, 949, 994
Eco130I CCWWGG 1 cut(s) 429
Eco147I AGGCCT 2 cut(s) 428, 1076
Eco57I CTGAAG 2 cut(s) 57, 155
EcoO109I RGGNCCY 1 cut(s) 736
EcoT14I CCWWGG 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 429
FauNDI CATATG 1 cut(s) 988
FbaI TGATCA 1 cut(s) 994
Fnu4HI GCNGC 2 cut(s) 714, 1200
FokI GGATG 4 cut(s) 233, 278, 335, 1311
Fsp4HI GCNGC 2 cut(s) 714, 1200
FspBI CTAG 1 cut(s) 44
GlaI GCGC 1 cut(s) 132
GluI GCNGC 2 cut(s) 714, 1200
GsuI CTGGAG 2 cut(s) 209, 287
HaeIII GGCC 7 cut(s) 41, 117, 428, 737, 1076, 1202, 1313
HapII CCGG 1 cut(s) 809
HhaI GCGC 1 cut(s) 133
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HincII GTYRAC 3 cut(s) 382, 612, 1272
HindII GTYRAC 3 cut(s) 382, 612, 1272
HindIII AAGCTT 1 cut(s) 888
HinfI GANTC 5 cut(s) 22, 122, 296, 727, 842
HpaI GTTAAC 1 cut(s) 612
HpaII CCGG 1 cut(s) 809
HphI GGTGA 3 cut(s) 77, 374, 736
Hpy166II GTNNAC 4 cut(s) 259, 382, 612, 1272
Hpy188I TCNGA 5 cut(s) 107, 112, 265, 732, 1241
Hpy188III TCNNGA 6 cut(s) 19, 304, 478, 496, 962, 1328
Hpy8I GTNNAC 4 cut(s) 259, 382, 612, 1272
Hpy99I CGWCG 1 cut(s) 1308
HpyAV CCTTC 1 cut(s) 727
HpyCH4III ACNGT 4 cut(s) 215, 825, 1009, 1132
HpyCH4IV ACGT 2 cut(s) 468, 1303
HpyCH4V TGCA 6 cut(s) 283, 353, 629, 716, 862, 1013
HpyF10VI GCNNNNNNNGC 7 cut(s) 434, 449, 923, 932, 1010, 1205, 1214
HpyF3I CTNAG 5 cut(s) 155, 453, 537, 558, 869
HpySE526I ACGT 2 cut(s) 468, 1303
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 131
Ksp22I TGATCA 1 cut(s) 994
KspAI GTTAAC 1 cut(s) 612
Kzo9I GATC 9 cut(s) 107, 138, 148, 165, 474, 492, 804, 949, 994
LmnI GCTCC 1 cut(s) 922
Lsp1109I GCAGC 1 cut(s) 700
MaeI CTAG 1 cut(s) 44
MaeII ACGT 2 cut(s) 468, 1303
MalI GATC 9 cut(s) 109, 140, 150, 167, 476, 494, 806, 951, 996
MboI GATC 9 cut(s) 107, 138, 148, 165, 474, 492, 804, 949, 994
MboII GAAGA 9 cut(s) 95, 148, 175, 250, 469, 472, 511, 529, 575
MflI RGATCY 2 cut(s) 165, 804
MhlI GDGCHC 1 cut(s) 547
MluCI AATT 7 cut(s) 57, 179, 365, 398, 525, 1230, 1254
MmeI TCCRAC 2 cut(s) 126, 1167
MseI TTAA 6 cut(s) 201, 390, 549, 611, 792, 1229
MslI CAYNNNNRTG 1 cut(s) 254
MspI CCGG 1 cut(s) 809
MspR9I CCNGG 1 cut(s) 809
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 7 cut(s) 434, 449, 923, 932, 1010, 1205, 1214
NciI CCSGG 1 cut(s) 809
NdeI CATATG 1 cut(s) 988
NdeII GATC 9 cut(s) 107, 138, 148, 165, 474, 492, 804, 949, 994
NlaIV GGNNCC 1 cut(s) 806
NspV TTCGAA 1 cut(s) 529
PceI AGGCCT 2 cut(s) 428, 1076
PfeI GAWTC 5 cut(s) 22, 122, 296, 727, 842
Pfl23II CGTACG 1 cut(s) 1132
PkrI GCNGC 2 cut(s) 715, 1201
Ple19I CGATCG 1 cut(s) 110
PsiI TTATAA 1 cut(s) 894
PspLI CGTACG 1 cut(s) 1132
PspN4I GGNNCC 1 cut(s) 806
PspPI GGNCC 3 cut(s) 40, 116, 736
PstI CTGCAG 2 cut(s) 285, 718
PsuI RGATCY 2 cut(s) 165, 804
PvuI CGATCG 1 cut(s) 110
RsaI GTAC 5 cut(s) 910, 1134, 1264, 1284, 1291
RsaNI GTAC 5 cut(s) 909, 1133, 1263, 1283, 1290
RseI CAYNNNNRTG 1 cut(s) 254
SaqAI TTAA 6 cut(s) 201, 390, 549, 611, 792, 1229
SatI GCNGC 2 cut(s) 714, 1200
Sau3AI GATC 9 cut(s) 107, 138, 148, 165, 474, 492, 804, 949, 994
Sau96I GGNCC 3 cut(s) 40, 116, 736
ScrFI CCNGG 1 cut(s) 809
SduI GDGCHC 1 cut(s) 547
SfcI CTRYAG 2 cut(s) 281, 714
SfuI TTCGAA 1 cut(s) 529
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 2 cut(s) 740, 753
SmoI CTYRAG 2 cut(s) 740, 753
Sse9I AATT 7 cut(s) 57, 179, 365, 398, 525, 1230, 1254
SseBI AGGCCT 2 cut(s) 428, 1076
SsiI CCGC 3 cut(s) 129, 1098, 1199
SspMI CTAG 1 cut(s) 44
StuI AGGCCT 2 cut(s) 428, 1076
StyD4I CCNGG 1 cut(s) 807
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 4 cut(s) 215, 825, 1009, 1132
TaiI ACGT 2 cut(s) 471, 1306
TaqI TCGA 5 cut(s) 141, 161, 177, 495, 529
TaqII GACCGA 1 cut(s) 19
TasI AATT 7 cut(s) 57, 179, 365, 398, 525, 1230, 1254
TatI WGTACW 2 cut(s) 908, 1262
TauI GCSGC 1 cut(s) 1202
TfiI GAWTC 5 cut(s) 22, 122, 296, 727, 842
Tru1I TTAA 6 cut(s) 201, 390, 549, 611, 792, 1229
Tru9I TTAA 6 cut(s) 201, 390, 549, 611, 792, 1229
TscAI CASTG 5 cut(s) 99, 830, 837, 1012, 1149
TseI GCWGC 1 cut(s) 713
TspDTI ATGAA 4 cut(s) 525, 611, 644, 1170
TspGWI ACGGA 1 cut(s) 1300
TspRI CASTG 5 cut(s) 99, 830, 837, 1012, 1149
XapI RAATTY 2 cut(s) 179, 525
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.