AT4G33980

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002473
Physical Location & Seq
Reverse (-)
16282281 .. 16285229
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33980.2

Sequence Viewer

Length: 657 bp
ATGGAGAATGATTGCACGGTGAATATTGTCTCTCTGGAGAAGGATCGCGATGTTTCGGAGGCGTCGGCTGAATCTCAGAGCGAGTCGACTCTTTCGAACTCGCTCGATTCCGGTGTTACGGCTGAGACCTCTCGTTCTGATGCTGATTCCAAACTGGATGAATGTACTGCTTGGACGAATGAGAAACACAACTCATATCTTGATTATTTAGAGAGCTCGTTTGTTAGGCAATTATACTCCTTGCTTGGAGGTGGGACTCAGAGACTTTCTAGAACTCGTGATGTGCAGTCTAACTCTCATAAATCAGCTGATCAGTTTACCGTCCTACAAAATGGTTGCTGGCAGAAGGTTAACTTTGGAAAGAAACAATCTTGTTTGGAGACTTCATCTGAGTTTCGTTTTCACAGAAATTCATTGAGAAATAAGCCTGAAAATTCCAACGGAAATTACACCATGGGAACTACTGTCCAAGGAGATGTGTTATGTCATGACGAAACCAAACACTCAGAGGCGTCAGGGCAGAATTTCAGAGAAGAAGAAGAAGAAGAAGAGAAGGGAGAGGTGAGCAAAAAACGAGAAAGAGAAGCAAATAACGATGATAGTTCATTGAAGGAGGATCAGGTTGTGCCGGTAAGGATGGTGAAGCCCAGAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

24.61

Weight (kDa)

4.97

Isoelectric Point (pI)

55.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 86
AccII CGCG 1 cut(s) 48
AclWI GGATC 2 cut(s) 51, 624
AcsI RAATTY 3 cut(s) 409, 433, 523
AcyI GRCGYC 2 cut(s) 62, 512
AfaI GTAC 1 cut(s) 166
AgsI TTSAA 1 cut(s) 610
AluBI AGCT 2 cut(s) 216, 308
AluI AGCT 2 cut(s) 216, 308
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 4 cut(s) 34, 119, 256, 374
AlwI GGATC 2 cut(s) 51, 624
ApoI RAATTY 3 cut(s) 409, 433, 523
AsuHPI GGTGA 3 cut(s) 31, 574, 652
AsuII TTCGAA 1 cut(s) 95
BanII GRGCYC 1 cut(s) 218
BauI CACGAG 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 218
BccI CCATC 1 cut(s) 631
BceAI ACGGC 1 cut(s) 135
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 4 cut(s) 34, 119, 256, 374
BfaI CTAG 1 cut(s) 270
BmsI GCATC 1 cut(s) 130
BpmI CTGGAG 1 cut(s) 56
Bpu14I TTCGAA 1 cut(s) 95
BsaHI GRCGYC 2 cut(s) 62, 512
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 2 cut(s) 453, 469
BsaWI WCCGGW 1 cut(s) 110
Bse118I RCCGGY 1 cut(s) 628
Bse1I ACTGG 1 cut(s) 159
BseDI CCNNGG 2 cut(s) 453, 469
BseGI GGATG 2 cut(s) 163, 642
BseMII CTCAG 5 cut(s) 89, 114, 272, 381, 519
BseNI ACTGG 1 cut(s) 159
BsgI GTGCAG 1 cut(s) 305
Bsh1236I CGCG 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 218
BsiSI CCGG 2 cut(s) 111, 629
BslFI GGGAC 1 cut(s) 268
BsmAI GTCTC 4 cut(s) 34, 119, 256, 374
BsmFI GGGAC 1 cut(s) 268
Bso31I GGTCTC 1 cut(s) 119
Bsp119I TTCGAA 1 cut(s) 95
Bsp1286I GDGCHC 1 cut(s) 218
Bsp143I GATC 3 cut(s) 43, 310, 616
Bsp19I CCATGG 1 cut(s) 453
Bsp68I TCGCGA 1 cut(s) 48
BspCNI CTCAG 5 cut(s) 88, 115, 271, 382, 518
BspFNI CGCG 1 cut(s) 48
BspHI TCATGA 1 cut(s) 487
BspPI GGATC 2 cut(s) 51, 624
BspT104I TTCGAA 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 119
BsrFI RCCGGY 1 cut(s) 628
BsrI ACTGG 1 cut(s) 159
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 2 cut(s) 453, 469
BssMI GATC 3 cut(s) 43, 310, 616
BssNI GRCGYC 2 cut(s) 62, 512
BssSI CACGAG 1 cut(s) 276
BssT1I CCWWGG 2 cut(s) 453, 469
Bst2BI CACGAG 1 cut(s) 276
Bst4CI ACNGT 3 cut(s) 19, 322, 466
Bst6I CTCTTC 1 cut(s) 543
BstACI GRCGYC 2 cut(s) 62, 512
BstBI TTCGAA 1 cut(s) 95
BstC8I GCNNGC 1 cut(s) 341
BstDEI CTNAG 5 cut(s) 75, 123, 258, 390, 505
BstDSI CCRYGG 1 cut(s) 453
BstF5I GGATG 2 cut(s) 163, 642
BstFNI CGCG 1 cut(s) 48
BstKTI GATC 3 cut(s) 46, 313, 619
BstMAI GTCTC 4 cut(s) 34, 119, 256, 374
BstMBI GATC 3 cut(s) 43, 310, 616
BstUI CGCG 1 cut(s) 48
BtgI CCRYGG 1 cut(s) 453
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 2 cut(s) 163, 642
BtuMI TCGCGA 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 341
CciI TCATGA 1 cut(s) 487
Cfr10I RCCGGY 1 cut(s) 628
CseI GACGC 2 cut(s) 51, 501
Csp6I GTAC 1 cut(s) 165
CviAII CATG 2 cut(s) 454, 488
CviJI RGCY 6 cut(s) 68, 122, 216, 308, 427, 646
CviKI_1 RGCY 6 cut(s) 68, 122, 216, 308, 427, 646
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 5 cut(s) 75, 123, 258, 390, 505
DpnI GATC 3 cut(s) 45, 312, 618
DpnII GATC 3 cut(s) 43, 310, 616
Eam1104I CTCTTC 1 cut(s) 543
EarI CTCTTC 1 cut(s) 543
Ecl136II GAGCTC 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 453, 469
Eco24I GRGCYC 1 cut(s) 218
Eco31I GGTCTC 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 216
EcoICRI GAGCTC 1 cut(s) 216
EcoT14I CCWWGG 2 cut(s) 453, 469
EcoT38I GRGCYC 1 cut(s) 218
ErhI CCWWGG 2 cut(s) 453, 469
FaeI CATG 2 cut(s) 457, 491
FaiI YATR 6 cut(s) 196, 235, 300, 455, 484, 489
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 1 cut(s) 268
FatI CATG 2 cut(s) 453, 487
FbaI TGATCA 1 cut(s) 310
FblI GTMKAC 1 cut(s) 86
FokI GGATG 2 cut(s) 170, 649
FriOI GRGCYC 1 cut(s) 218
FspBI CTAG 1 cut(s) 270
GsuI CTGGAG 1 cut(s) 56
HapII CCGG 2 cut(s) 111, 629
HgaI GACGC 2 cut(s) 51, 501
Hin1I GRCGYC 2 cut(s) 62, 512
Hin1II CATG 2 cut(s) 457, 491
HincII GTYRAC 2 cut(s) 87, 352
HindII GTYRAC 2 cut(s) 87, 352
HinfI GANTC 6 cut(s) 71, 83, 88, 107, 146, 256
HpaI GTTAAC 1 cut(s) 352
HpaII CCGG 2 cut(s) 111, 629
HphI GGTGA 3 cut(s) 31, 574, 652
Hpy166II GTNNAC 3 cut(s) 87, 318, 352
Hpy188I TCNGA 7 cut(s) 58, 78, 139, 261, 391, 508, 530
Hpy188III TCNNGA 6 cut(s) 35, 47, 200, 270, 278, 488
Hpy8I GTNNAC 3 cut(s) 87, 318, 352
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 4 cut(s) 34, 340, 547, 604
HpyCH4III ACNGT 3 cut(s) 19, 322, 466
HpyCH4IV ACGT 1 cut(s) 653
HpyCH4V TGCA 2 cut(s) 15, 286
HpyF3I CTNAG 5 cut(s) 75, 123, 258, 390, 505
HpySE526I ACGT 1 cut(s) 653
Hsp92I GRCGYC 2 cut(s) 62, 512
Hsp92II CATG 2 cut(s) 457, 491
Ksp22I TGATCA 1 cut(s) 310
KspAI GTTAAC 1 cut(s) 352
Kzo9I GATC 3 cut(s) 43, 310, 616
LpnPI CCDG 8 cut(s) 20, 124, 140, 325, 441, 501, 605, 642
LweI GCATC 1 cut(s) 130
MaeI CTAG 1 cut(s) 270
MaeII ACGT 1 cut(s) 653
MaeIII GTNAC 1 cut(s) 115
MalI GATC 3 cut(s) 45, 312, 618
MboI GATC 3 cut(s) 43, 310, 616
MboII GAAGA 6 cut(s) 545, 548, 551, 554, 557, 560
MhlI GDGCHC 1 cut(s) 218
MluCI AATT 5 cut(s) 230, 409, 433, 445, 523
MlyI GAGTC 3 cut(s) 82, 92, 250
MmeI TCCRAC 1 cut(s) 462
MnlI CCTC 6 cut(s) 52, 139, 242, 502, 553, 607
MseI TTAA 1 cut(s) 351
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 2 cut(s) 111, 629
MvnI CGCG 1 cut(s) 48
NcoI CCATGG 1 cut(s) 453
NdeII GATC 3 cut(s) 43, 310, 616
NlaIII CATG 2 cut(s) 457, 491
NruI TCGCGA 1 cut(s) 48
NspV TTCGAA 1 cut(s) 95
PagI TCATGA 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PfeI GAWTC 3 cut(s) 71, 107, 146
PleI GAGTC 3 cut(s) 82, 91, 250
PpsI GAGTC 3 cut(s) 82, 91, 250
Psp124BI GAGCTC 1 cut(s) 218
PvuII CAGCTG 1 cut(s) 308
RruI TCGCGA 1 cut(s) 48
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
SacI GAGCTC 1 cut(s) 218
SalI GTCGAC 1 cut(s) 85
SaqAI TTAA 1 cut(s) 351
Sau3AI GATC 3 cut(s) 43, 310, 616
SchI GAGTC 3 cut(s) 82, 92, 250
SduI GDGCHC 1 cut(s) 218
SetI ASST 8 cut(s) 131, 218, 253, 310, 351, 564, 624, 656
SfaNI GCATC 1 cut(s) 130
SfuI TTCGAA 1 cut(s) 95
Sse9I AATT 5 cut(s) 230, 409, 433, 445, 523
SspI AATATT 1 cut(s) 25
SspMI CTAG 1 cut(s) 270
SstI GAGCTC 1 cut(s) 218
StyI CCWWGG 2 cut(s) 453, 469
TaaI ACNGT 3 cut(s) 19, 322, 466
TaiI ACGT 1 cut(s) 656
TaqI TCGA 3 cut(s) 86, 95, 105
TasI AATT 5 cut(s) 230, 409, 433, 445, 523
TatI WGTACW 1 cut(s) 164
TfiI GAWTC 3 cut(s) 71, 107, 146
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TspDTI ATGAA 4 cut(s) 174, 375, 402, 594
TspGWI ACGGA 1 cut(s) 456
XapI RAATTY 3 cut(s) 409, 433, 523
XbaI TCTAGA 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 86
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.