Rh2DG395200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002473
Physical Location & Seq
Forward (+)
57466173 .. 57471411
5239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG395200.1

Sequence Viewer

Length: 1218 bp
ATGGATCCTGTAACTGTTGCAAGTCTACTGACTGCTTGTGCACAATCAGATGATTCCTTAAGTGGGATGCTGATTCATTTGTATGTCATAAAGCATGGACTAGAGTTTGATCTGTTCGTATGCAATGCCTTGATTAACATGTATGCTAAATTTGGTTGCTTGGGACATGCACACAGGGTTTTTTATGAAATGCAGGTAAGAGATTTGGTGTCATGGAACTCAATAATTACTGCAAATGAGCAGAATGATGAGCCGATGACTGCGCTTGGATTCTTCAACAGAATGCAACTATCCGGAGTTAAGCCTGATTGGTTAACACTAGTGAGTTTGGCTTCCATTATAGCTCAATTAAATGATGCTGAAATGAGTAGGTCTGTTCATGGATTCATTTTGAGGAGAGATTGGTTCAAGGAAGATGTTGTTATTGGAAATGCAGTTGTGGACATGTATGCAAAGTTGGGTGCTGTAGATTCTGCACGTACTGTTTTTGAAGGACTTCCTGTCAAGGATGTGGTTTCATGGAATACTTTGATCACAGAGTGGACAAATGAGAAGCACAGATCATATATAAATTCCTTAGAAACATCATTTGTAAATAAATTGTACCATTCCTGGCATTTGCAGGACTGCCATCCACAAAAGTATGTTCGGGGGACACATCCATCAGAAGAGTTGCCTTTCAAGACTTGTAATTCTTCTGACCAGGTCAGGGAGTCAAATCTGTTCATTGTTCTTCGAGATAGGCGTTCTCAGAAGATCATCTCTCCTATGAATGAAACTCTGCTGGAAAGCACCGCTGATTCTAATGTTACTATGGAAAGGCCCCGGATGGATCATATAGTGTCTTGTTATAAGGGGACCCACCTAAGAAGGAAACCGACCTCAGAACACTCAGGTGTAATCGCAAGAACTTCAGAGCAGAACCCTGCATGTCATCAGGATTCAGTTGGCAGTGCTGCAGAAGTTTCGGGTCAGAACTTTGTGGATGAGGACAACAGAGGGAAGCTAAACTGTGAATTCATACCAAAAAGGCTTAAAAGGGCTTCACCCAACTCTTCCAGCAATGATCAACTTGTGCCATTGGGAAATTTTGATACAAAGGAGGTTTCAATGGCCCATAATGCTCCCTCCGGAAGAGAAGAACAAGGGCATCATCAGTTGCTATTAGAGCACCTTGAGAGCTTCATCTGTCCAAGATCTATCTTCACTGATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

405

Amino Acids

45.74

Weight (kDa)

5.97

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 68 - 108 9.4e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 852
Acc36I ACCTGC 1 cut(s) 184
AccI GTMKAC 1 cut(s) 25
AccIII TCCGGA 2 cut(s) 293, 1130
AciI CCGC 1 cut(s) 795
AclWI GGATC 2 cut(s) 12, 840
AcsI RAATTY 4 cut(s) 149, 571, 1016, 1087
AcuI CTGAAG 1 cut(s) 897
AdeI CACNNNGTG 1 cut(s) 540
AfaI GTAC 2 cut(s) 481, 605
AfiI CCNNNNNNNGG 2 cut(s) 63, 709
AflII CTTAAG 1 cut(s) 58
AflIII ACRYGT 2 cut(s) 138, 444
AgsI TTSAA 5 cut(s) 277, 409, 491, 682, 1110
AhdI GACNNNNNGTC 1 cut(s) 500
AhlI ACTAGT 1 cut(s) 319
AjnI CCWGG 2 cut(s) 611, 702
AleI CACNNNNGTG 1 cut(s) 894
AluBI AGCT 3 cut(s) 344, 1006, 1182
AluI AGCT 3 cut(s) 344, 1006, 1182
Alw21I GWGCWC 2 cut(s) 43, 1173
Alw44I GTGCAC 1 cut(s) 39
AlwI GGATC 2 cut(s) 12, 840
Aor13HI TCCGGA 2 cut(s) 293, 1130
AoxI GGCC 2 cut(s) 821, 1113
ApaLI GTGCAC 1 cut(s) 39
ApeKI GCWGC 1 cut(s) 956
ApoI RAATTY 4 cut(s) 149, 571, 1016, 1087
Asp700I GAANNNNTTC 1 cut(s) 495
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 3 cut(s) 822, 858, 1114
AsuC2I CCSGG 1 cut(s) 826
AsuHPI GGTGA 1 cut(s) 1038
AvaII GGWCC 1 cut(s) 858
BaeGI GKGCMC 1 cut(s) 43
BamHI GGATCC 1 cut(s) 4
Bbv12I GWGCWC 2 cut(s) 43, 1173
BbvI GCAGC 1 cut(s) 943
BccI CCATC 3 cut(s) 639, 670, 823
BcgI CGANNNNNNTGC 2 cut(s) 948, 982
BciT130I CCWGG 2 cut(s) 613, 704
BclI TGATCA 2 cut(s) 531, 1066
BcnI CCSGG 1 cut(s) 826
BcuI ACTAGT 1 cut(s) 319
BfaI CTAG 2 cut(s) 101, 320
BfmI CTRYAG 2 cut(s) 465, 957
BfrI CTTAAG 1 cut(s) 58
BfuAI ACCTGC 1 cut(s) 184
BglII AGATCT 1 cut(s) 1196
BisI GCNGC 1 cut(s) 957
BlsI GCNGC 1 cut(s) 958
Bme1390I CCNGG 3 cut(s) 613, 704, 826
Bme18I GGWCC 1 cut(s) 858
BmeRI GACNNNNNGTC 1 cut(s) 500
BmgT120I GGNCC 3 cut(s) 822, 858, 1114
BmiI GGNNCC 4 cut(s) 6, 824, 859, 860
BmrFI CCNGG 3 cut(s) 613, 704, 826
BmsI GCATC 3 cut(s) 57, 346, 1159
BpuEI CTTGAG 1 cut(s) 1196
BpuMI CCSGG 1 cut(s) 826
BsaAI YACGTR 1 cut(s) 479
BsaJI CCNNGG 1 cut(s) 824
BsaWI WCCGGW 2 cut(s) 293, 1130
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 709
Bse3DI GCAATG 2 cut(s) 130, 1069
BseAI TCCGGA 2 cut(s) 293, 1130
BseBI CCWGG 2 cut(s) 613, 704
BseDI CCNNGG 1 cut(s) 824
BseGI GGATG 6 cut(s) 72, 514, 631, 658, 834, 991
BseLI CCNNNNNNNGG 2 cut(s) 63, 709
BseMI GCAATG 2 cut(s) 130, 1069
BseMII CTCAG 3 cut(s) 764, 897, 906
BseRI GAGGAG 1 cut(s) 409
BseSI GKGCMC 1 cut(s) 43
BseXI GCAGC 1 cut(s) 943
BsgI GTGCAG 1 cut(s) 459
BshFI GGCC 2 cut(s) 823, 1115
BsiHKAI GWGCWC 2 cut(s) 43, 1173
BsiSI CCGG 3 cut(s) 294, 826, 1131
BslFI GGGAC 3 cut(s) 177, 667, 871
BslI CCNNNNNNNGG 2 cut(s) 63, 709
BsmFI GGGAC 3 cut(s) 177, 667, 871
BsmI GAATGC 1 cut(s) 288
BsnI GGCC 2 cut(s) 823, 1115
Bsp1286I GDGCHC 2 cut(s) 43, 1173
Bsp13I TCCGGA 2 cut(s) 293, 1130
Bsp143I GATC 8 cut(s) 4, 109, 531, 560, 756, 832, 1066, 1196
BspACI CCGC 1 cut(s) 795
BspANI GGCC 2 cut(s) 823, 1115
BspCNI CTCAG 3 cut(s) 763, 896, 905
BspEI TCCGGA 2 cut(s) 293, 1130
BspLI GGNNCC 4 cut(s) 6, 824, 859, 860
BspMAI CTGCAG 1 cut(s) 961
BspMI ACCTGC 1 cut(s) 184
BspPI GGATC 2 cut(s) 12, 840
BspTI CTTAAG 1 cut(s) 58
BsrDI GCAATG 2 cut(s) 130, 1069
BssECI CCNNGG 1 cut(s) 824
BssMI GATC 8 cut(s) 4, 109, 531, 560, 756, 832, 1066, 1196
Bst2UI CCWGG 2 cut(s) 613, 704
Bst4CI ACNGT 3 cut(s) 16, 484, 1013
Bst6I CTCTTC 3 cut(s) 663, 1060, 1129
BstAFI CTTAAG 1 cut(s) 58
BstBAI YACGTR 1 cut(s) 479
BstDEI CTNAG 6 cut(s) 577, 750, 866, 883, 892, 1215
BstF5I GGATG 6 cut(s) 72, 514, 631, 658, 834, 991
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 8 cut(s) 7, 112, 534, 563, 759, 835, 1069, 1199
BstMBI GATC 8 cut(s) 4, 109, 531, 560, 756, 832, 1066, 1196
BstMWI GCNNNNNNNGC 2 cut(s) 1121, 1168
BstNI CCWGG 2 cut(s) 613, 704
BstNSI RCATGY 4 cut(s) 142, 170, 448, 933
BstSCI CCNGG 3 cut(s) 611, 702, 824
BstSFI CTRYAG 2 cut(s) 465, 957
BstSLI GKGCMC 1 cut(s) 43
BstV1I GCAGC 1 cut(s) 943
BstX2I RGATCY 2 cut(s) 4, 1196
BstYI RGATCY 2 cut(s) 4, 1196
BsuRI GGCC 2 cut(s) 823, 1115
BtsCI GGATG 6 cut(s) 72, 514, 631, 658, 834, 991
BtsI GCAGTG 1 cut(s) 958
BtsIMutI CAGTG 2 cut(s) 958, 1206
BveI ACCTGC 1 cut(s) 184
CfoI GCGC 1 cut(s) 265
Cfr13I GGNCC 3 cut(s) 822, 858, 1114
CsiI ACCWGGT 1 cut(s) 702
Csp6I GTAC 2 cut(s) 480, 604
CviAII CATG 8 cut(s) 95, 139, 167, 213, 380, 445, 519, 930
CviQI GTAC 2 cut(s) 480, 604
DdeI CTNAG 6 cut(s) 577, 750, 866, 883, 892, 1215
DpnI GATC 8 cut(s) 6, 111, 533, 562, 758, 834, 1068, 1198
DpnII GATC 8 cut(s) 4, 109, 531, 560, 756, 832, 1066, 1196
DraIII CACNNNGTG 1 cut(s) 540
DriI GACNNNNNGTC 1 cut(s) 500
Eam1104I CTCTTC 3 cut(s) 663, 1060, 1129
Eam1105I GACNNNNNGTC 1 cut(s) 500
EarI CTCTTC 3 cut(s) 663, 1060, 1129
Eco47I GGWCC 1 cut(s) 858
Eco57I CTGAAG 1 cut(s) 897
EcoO109I RGGNCCY 2 cut(s) 822, 858
EcoRI GAATTC 1 cut(s) 1016
EcoRII CCWGG 2 cut(s) 611, 702
FaeI CATG 8 cut(s) 98, 142, 170, 216, 383, 448, 522, 933
FaqI GGGAC 3 cut(s) 177, 667, 871
FatI CATG 8 cut(s) 94, 138, 166, 212, 379, 444, 518, 929
FbaI TGATCA 2 cut(s) 531, 1066
FblI GTMKAC 1 cut(s) 25
Fnu4HI GCNGC 1 cut(s) 957
FokI GGATG 6 cut(s) 79, 521, 618, 645, 841, 998
Fsp4HI GCNGC 1 cut(s) 957
FspBI CTAG 2 cut(s) 101, 320
GlaI GCGC 1 cut(s) 264
GluI GCNGC 1 cut(s) 957
HaeIII GGCC 2 cut(s) 823, 1115
HapII CCGG 3 cut(s) 294, 826, 1131
HhaI GCGC 1 cut(s) 265
Hin1II CATG 8 cut(s) 98, 142, 170, 216, 383, 448, 522, 933
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HincII GTYRAC 1 cut(s) 315
HindII GTYRAC 1 cut(s) 315
HinfI GANTC 9 cut(s) 53, 73, 270, 384, 470, 713, 800, 941, 1211
HpaI GTTAAC 1 cut(s) 315
HpaII CCGG 3 cut(s) 294, 826, 1131
HphI GGTGA 1 cut(s) 1038
Hpy166II GTNNAC 5 cut(s) 26, 41, 315, 442, 543
Hpy188I TCNGA 7 cut(s) 49, 667, 700, 753, 886, 916, 975
Hpy188III TCNNGA 5 cut(s) 294, 682, 737, 938, 1131
Hpy8I GTNNAC 5 cut(s) 26, 41, 315, 442, 543
HpyAV CCTTC 2 cut(s) 485, 864
HpyCH4III ACNGT 3 cut(s) 16, 484, 1013
HpyCH4IV ACGT 1 cut(s) 478
HpyF10VI GCNNNNNNNGC 2 cut(s) 1121, 1168
HpyF3I CTNAG 6 cut(s) 577, 750, 866, 883, 892, 1215
HpySE526I ACGT 1 cut(s) 478
Hsp92II CATG 8 cut(s) 98, 142, 170, 216, 383, 448, 522, 933
HspAI GCGC 1 cut(s) 263
KflI GGGWCCC 1 cut(s) 858
Kpn2I TCCGGA 2 cut(s) 293, 1130
Ksp22I TGATCA 2 cut(s) 531, 1066
KspAI GTTAAC 1 cut(s) 315
Kzo9I GATC 8 cut(s) 4, 109, 531, 560, 756, 832, 1066, 1196
LmnI GCTCC 1 cut(s) 1129
Lsp1109I GCAGC 1 cut(s) 943
LweI GCATC 3 cut(s) 57, 346, 1159
MabI ACCWGGT 1 cut(s) 702
MaeI CTAG 2 cut(s) 101, 320
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 2 cut(s) 10, 808
MalI GATC 8 cut(s) 6, 111, 533, 562, 758, 834, 1068, 1198
MboI GATC 8 cut(s) 4, 109, 531, 560, 756, 832, 1066, 1196
MflI RGATCY 2 cut(s) 4, 1196
MhlI GDGCHC 2 cut(s) 43, 1173
MluCI AATT 8 cut(s) 149, 225, 347, 571, 599, 691, 1016, 1087
MlyI GAGTC 1 cut(s) 722
MnlI CCTC 6 cut(s) 387, 892, 982, 992, 1096, 1138
MroI TCCGGA 2 cut(s) 293, 1130
MroXI GAANNNNTTC 1 cut(s) 495
MseI TTAA 6 cut(s) 59, 135, 300, 314, 350, 1035
MslI CAYNNNNRTG 2 cut(s) 81, 894
MspA1I CMGCKG 1 cut(s) 797
MspCI CTTAAG 1 cut(s) 58
MspI CCGG 3 cut(s) 294, 826, 1131
MspR9I CCNGG 3 cut(s) 613, 704, 826
Mva1269I GAATGC 1 cut(s) 288
MvaI CCWGG 2 cut(s) 613, 704
MwoI GCNNNNNNNGC 2 cut(s) 1121, 1168
NciI CCSGG 1 cut(s) 826
NdeII GATC 8 cut(s) 4, 109, 531, 560, 756, 832, 1066, 1196
NlaIII CATG 8 cut(s) 98, 142, 170, 216, 383, 448, 522, 933
NlaIV GGNNCC 4 cut(s) 6, 824, 859, 860
NspI RCATGY 4 cut(s) 142, 170, 448, 933
OliI CACNNNNGTG 1 cut(s) 894
PciI ACATGT 2 cut(s) 138, 444
PcsI WCGNNNNNNNCGW 1 cut(s) 742
PctI GAATGC 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 495
PfeI GAWTC 8 cut(s) 53, 73, 270, 384, 470, 800, 941, 1211
PflFI GACNNNGTC 1 cut(s) 704
PkrI GCNGC 1 cut(s) 958
PleI GAGTC 1 cut(s) 721
PpsI GAGTC 1 cut(s) 721
Ppu21I YACGTR 1 cut(s) 479
PpuMI RGGWCCY 1 cut(s) 858
PscI ACATGT 2 cut(s) 138, 444
PsiI TTATAA 1 cut(s) 852
Psp5II RGGWCCY 1 cut(s) 858
Psp6I CCWGG 2 cut(s) 611, 702
PspGI CCWGG 2 cut(s) 611, 702
PspN4I GGNNCC 4 cut(s) 6, 824, 859, 860
PspPI GGNCC 3 cut(s) 822, 858, 1114
PspPPI RGGWCCY 1 cut(s) 858
PstI CTGCAG 1 cut(s) 961
PsuI RGATCY 2 cut(s) 4, 1196
PsyI GACNNNGTC 1 cut(s) 704
RsaI GTAC 2 cut(s) 481, 605
RsaNI GTAC 2 cut(s) 480, 604
RseI CAYNNNNRTG 2 cut(s) 81, 894
SaqAI TTAA 6 cut(s) 59, 135, 300, 314, 350, 1035
SatI GCNGC 1 cut(s) 957
Sau3AI GATC 8 cut(s) 4, 109, 531, 560, 756, 832, 1066, 1196
Sau96I GGNCC 3 cut(s) 822, 858, 1114
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 3 cut(s) 613, 704, 826
SduI GDGCHC 2 cut(s) 43, 1173
SexAI ACCWGGT 1 cut(s) 702
SfaNI GCATC 3 cut(s) 57, 346, 1159
SfcI CTRYAG 2 cut(s) 465, 957
SinI GGWCC 1 cut(s) 858
SmiMI CAYNNNNRTG 2 cut(s) 81, 894
SmlI CTYRAG 2 cut(s) 58, 1175
SmoI CTYRAG 2 cut(s) 58, 1175
SpeI ACTAGT 1 cut(s) 319
Sse9I AATT 8 cut(s) 149, 225, 347, 571, 599, 691, 1016, 1087
SsiI CCGC 1 cut(s) 795
SspMI CTAG 2 cut(s) 101, 320
StyD4I CCNGG 3 cut(s) 611, 702, 824
TaaI ACNGT 3 cut(s) 16, 484, 1013
TaiI ACGT 1 cut(s) 481
TaqI TCGA 1 cut(s) 736
TasI AATT 8 cut(s) 149, 225, 347, 571, 599, 691, 1016, 1087
TfiI GAWTC 8 cut(s) 53, 73, 270, 384, 470, 800, 941, 1211
Tru1I TTAA 6 cut(s) 59, 135, 300, 314, 350, 1035
Tru9I TTAA 6 cut(s) 59, 135, 300, 314, 350, 1035
TscAI CASTG 2 cut(s) 958, 1213
TseI GCWGC 1 cut(s) 956
TspRI CASTG 2 cut(s) 958, 1213
Tth111I GACNNNGTC 1 cut(s) 704
Vha464I CTTAAG 1 cut(s) 58
VneI GTGCAC 1 cut(s) 39
VpaK11BI GGWCC 1 cut(s) 858
XapI RAATTY 4 cut(s) 149, 571, 1016, 1087
XceI RCATGY 4 cut(s) 142, 170, 448, 933
XmiI GTMKAC 1 cut(s) 25
XmnI GAANNNNTTC 1 cut(s) 495
XspI CTAG 2 cut(s) 101, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.