Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002744

Physical Location & Seq

Genomic Coordinates Forward (+)

16865304 .. 16871527

Gene Structure 6224 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G35520.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3468 bp

ATGAAGACGATCAAGCCCTTGCCGGAAGGAGTTCGTCACTCCATGCGTTCTGGAATTATCATGTTCGACATGGCGAGGGTCGTGGAAGAACTCGTCTTCAACAGTCTCGATGCTGGGGCGACCAAGGTGTCTATCTTCGTGGGTGTTGTTTCATGCTCTGTGAAAGTTGTGGATGATGGATCAGGCGTTTCAAGAGATGATTTGGTTTTGTTGGGAGAAAGATATGCTACTTCAAAGTTTCACGACTTCACCAACGTGGAGACAGCTAGTGAAACTTTTGGATTTCGTGGAGAGGCCTTAGCTTCAATATCAGATATCTCGTTACTGGAGGTTAGGACAAAAGCTATTGGGAGGCCTAATGGTTATCGAAAGGTTATGAAGGGATCCAAGTGTCTACATCTTGGAATTGATGATGATAGAAAAGACTCTGGCACGACGGTAACTGTCCGAGATCTATTTTACAGTCAGCCAGTGAGACGAAAATATATGCAATCCAGCCCCAAGAAAGTTTTGGAATCTATCAAAAAGTGTGTGTTCCGGATTGCCCTTGTGCACTCCAATGTTTCCTTCAGTGTTCTTGATATCGAAAGTGATGAAGAGCTTTTCCAAACCAATCCTTCTTCTTCAGCATTCTCACTACTGATGAGAGATGCAGGGACCGAAGCTGTAAATTCGCTTTGTAAAGTAAACGTTACAGATGGCATGCTGAATGTCTCTGGTTTTGAGTGTGCGGATGACTGGAAGCCTACGGATGGGCAACAAACAGGAAGACGCAATAGACTTCAATCCAACCCTGGTTACATTCTGTGCATAGCATGTCCACGCCGTCTTTATGAATTCTCGTTTGAACCATCAAAGACGCACGTTGAGTTCAAGAAGTGGGGACCTGTACTTGCCTTTATAGAAAGAATCACTCTAGCCAACTGGAAGAAAGATAGAATTCTTGAACTTTTTGATGGGGGAGCTGATATACTGGCAAAAGGTGATAGACAAGACCTGATTGATGACAAAATTAGACTTCAAAACGGCAGCCTTTTCTCAATTCTTCATTTTCTGGATGCAGATTGGCCAGAAGCTATGGAACCTGCAAAAAAGAAGCTGAAGAGAAGTAATGATCATGCACCTTGTAGTTCTCTCTTGTTTCCGTCTGCTGACTTTAAACAAGATGGTGATTATTTTTCTCCACGAAAGGATGTATGGTCTCCAGAATGTGAAGTCGAACTGAAAATTCAGAATCCCAAAGAGCAAGGTACTGTAGCTGGATTTGAAAGCCGGACTGATTCTCTTCTACAGTCACGTGACATAGAAATGCAAACGAATGAAGACTTCCCACAAGTTACTGACCTCCTTGAAACAAGCTTGGTTGCTGACTCTAAGTGCCGTAAACAGTTTCTAACAAGATGTCAGATTACCACACCTGTCAATATCAACCATGATTTTATGAAAGATTCAGACGTGTTAAATTTTCAGTTTCAAGGATTGAAAGATGAGTTGGATGTCAGCAATTGCATTGGAAAGCATCTCTTGCGTGGTTGCTCTTCAAGAGTAAGCCTAACCTTTCATGAGCCTAAACTATCTCATGTTGAAGGGTATGAATCCGTCGTGCCTATGATACCTAATGAAAAACAAAGTAGTCCGCGGGTCCTAGAGACCAGAGAAGGTGGTTCGTACTGTGATGTTTATTCTGATAAGACTCCTGATTGTTCCCTAGGGAGTTCATGGCAGGATACTGATTGGTTTACTCCACAGTGTTCCTCAGATAGGGGATGTGTTGGAATTGGAGAAGATTTTAACATTACCCCCATAGATACTGCGGAATTTGATTCTTATGATGAAAAAGTTGGTAGTAAAAAGTATCTTTCTTCTGTCAATGTGGGGAGCTCTGTTACTGGTAGTTTCTGTTTAAGTTCTGAGTGGTCTCCAATGTACTCCACACCTTCTGCGACCAAGTGGGAGTCTGAGTACCAGAAAGGTTGTCGAATTCTTGAACAGAGTTTGAGACTGGGAAGGATGCCTGACCCTGAATTTTGTTTCAGTGCAGCTAACAACATCAAATTTGACCACGAGGTCATACCTGAAATGGATTGCTGTGAAACCGGTACAGACTCTTTCACAGCTATTCAGAACTGCACTCAGTTAGCTGATAAAATTTGCAAGTCTTCGTGGGGGCATGCAGATGATGTGCGTATTGACCAATATAGTATCAGGAAGGAAAAGTTCAGTTATATGGATGGCACACAGAACAATGCTGGTAAACAAAGGTCAAAAAGAAGTCGATCTGCTCCTCCATTTTATCGAGAGAAGAAGAGATTTATCAGCTTAAGTTGTAAATCAGACACAAAACCAAAGAACTCTGATCCATCAGAACCTGATGATCTGGAGTGTTTGACACAACCTTGTAATGCATCTCAAATGCATCTTAAGTGCAGCATCCTTGATGATGTGTCGTATGACCACATACAAGAAACAGAAAAAAGATTGAGTTCTGCCTCAGACTTGAAAGCATCTGCTGGTTGCAGGACTGTGCACTCAGAGACCCAAGATGAGGATGTGCACGAAGACTTCAGCTCAGAGGAATTTCTGGATCCAATTAAATCCACAACAAAATGGCGCCATAACTGTGCGGTCTCTCAGGTTCCCAAGGAATCACACGAGCTTCATGGTCAAGATGGTGTATTTGATATATCTTCGGGACTTCTGCACTTACGATCCGATGAATCCTTGGTTCCTGAATCTATCAACAGACACTCCCTTGAAGATGCCAAGGTTCTACAACAGGTTGATAAAAAATATATCCCAATCGTTGCTTGTGGAACAGTTGCCATCGTTGATCAGCATGCTGCCGATGAAAGAATTCGTTTGGAAGAGCTGCGTACAAAGGTCCTGGCAGGGAAAGCGAGGACAGTCACCTACTTGAGTGCAGACCAAGAGTTGGTACTGCCGGAGATGGGTTATCAGTTACTCCAGAGTTATTCAGAGCAGATAAGAGACTGGGGTTGGATCTGCAACATTACTGTAGAAGGGTCAACGTCCTTTAAGAAAAACATGAGCATCATCCAGCGGAAACCAACACCAATCACACTTAATGCGGTTCCATGCATTCTGGGTGTAAATCTATCAGATGTTGATCTATTAGAGTTTCTTCAGCAGCTTGCTGATACTGACGGATCATCAACTATTCCTCCATCTGTTCTTCGAGTCCTAAATTCCAAAGCCTGTAGAGGTGCAATTATGTTTGGAGATAGTCTGTTACCGTCAGAATGCTCTTTAATCATTGATGGACTGAAGCAGACCTCACTTTGTTTCCAGTGTGCTCATGGGCGACCTACAACAGTTCCTCTTGTCGATTTGAAGGCATTGCACAAACAGATAGCAAAGCTCAGTGGAAGACAAGTGTGGCATGGCTTACAACGCAGAGAAATTACACTTGATCGTGCAAAATCACGCTTAGACAACGCTAAAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000793 GO:0000794 GO:0000795 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005712 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022414 GO:0031974 GO:0031981 GO:0032300 GO:0032991 GO:0033554 GO:0034641 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048285 GO:0050896 GO:0051321 GO:0051716 GO:0061982 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0099086 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko03430 map03430

Pfam Domains

Protein Families

DNA_mis_repair HATPase_c_3 MutL_C

Protein Analysis

1155

Amino Acids

129.28

Weight (kDa)

5.66

Isoelectric Point (pI)

44.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c_3	PF13589	24 - 120	2.3e-08	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
MutL_C	PF08676	949 - 1108	3.3e-10	MutL C terminal dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35520 AT4G35520 AT4G35520 AT4G35520 AT4G35520 AT4G35520 AT4G35520 AT4G35520
fragaria_vesca	FvH4_1g10270 FvH4_1g10270 FvH4_1g10270 FvH4_1g10270 FvH4_1g10270 FvH4_1g10270 FvH4_1g10270 FvH4_1g10270
malus_domestica	MD02G1114700.v1.1 MD15G1230500.v1.1 MD15G1230600.v1.1
prunus_persica	Prupe.7G183500_v2.0.a1 Prupe.7G183500_v2.0.a1 Prupe.7G183500_v2.0.a1 Prupe.7G183500_v2.0.a1 Prupe.7G183500_v2.0.a1 Prupe.7G183500_v2.0.a1
pyrus_communis	pycom02g08960 pycom15g20550
rosa_chinensis	RchiOBHm_Chr2g0097091
rosa_laevigata	RLG00000016702
rosa_multiflora	Rmu_co8451989.1_g000001 Rmu_sc0024596.1_g000006
rosa_roxburghii	Rroxscaffold_2G00144980
rosa_rugosa	Rorug02G0063600 Rorug02G0063700
rosa_samantha	Rh2AG111200 Rh2BG113100 Rh2CG115300 Rh2DG114500
rosa_wichuraiana	Rw2G008650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	127, 2066
Acc36I	ACCTGC	1 cut(s)	1093
AccB1I	GGYRCC	1 cut(s)	2610
AccB7I	CCANNNNNTGG	1 cut(s)	2932
AccI	GTMKAC	1 cut(s)	394
AccII	CGCG	1 cut(s)	1639
AccIII	TCCGGA	1 cut(s)	537
AciI	CCGC	7 cut(s)	731, 1637, 1639, 1814, 2624, 3061, 3089
AclI	AACGTT	1 cut(s)	690
AclWI	GGATC	9 cut(s)	187, 378, 391, 2351, 2579, 2592, 2703, 3008, 3175
AcoI	YGGCCR	1 cut(s)	1067
AcuI	CTGAAG	6 cut(s)	553, 609, 1121, 2548, 3128, 3305
AcvI	CACGTG	1 cut(s)	1298
AcyI	GRCGYC	1 cut(s)	2611
AfaI	GTAC	8 cut(s)	891, 1252, 1670, 1928, 1964, 2101, 2875, 2937
AfiI	CCNNNNNNNGG	5 cut(s)	752, 1761, 2545, 2932, 2948
AflII	CTTAAG	2 cut(s)	2320, 2420
AflIII	ACRYGT	1 cut(s)	1455
AgeI	ACCGGT	1 cut(s)	2096
AjiI	CACGTC	1 cut(s)	1456
AjnI	CCWGG	2 cut(s)	793, 2883
AjuI	GAANNNNNNNTTGG	4 cut(s)	551, 583, 1475, 1507
AleI	CACNNNNGTG	1 cut(s)	254
Alw21I	GWGCWC	5 cut(s)	555, 1883, 2529, 2556, 3316
Alw44I	GTGCAC	3 cut(s)	551, 2525, 2552
AlwI	GGATC	9 cut(s)	187, 378, 391, 2351, 2579, 2592, 2703, 3008, 3175
AlwNI	CAGNNNCTG	1 cut(s)	2369
Aor13HI	TCCGGA	1 cut(s)	537
AoxI	GGCC	3 cut(s)	294, 353, 1067
ApaLI	GTGCAC	3 cut(s)	551, 2525, 2552
ApeKI	GCWGC	6 cut(s)	1029, 2039, 2427, 2840, 2869, 3148
AsiGI	ACCGGT	1 cut(s)	2096
Asp700I	GAANNNNTTC	2 cut(s)	30, 2853
AspA2I	CCTAGG	1 cut(s)	1708
AspLEI	GCGC	1 cut(s)	2613
AspS9I	GGNCC	4 cut(s)	657, 884, 1642, 2881
AsuHPI	GGTGA	4 cut(s)	241, 995, 1181, 2899
AvaII	GGWCC	4 cut(s)	657, 884, 1642, 2881
AvrII	CCTAGG	1 cut(s)	1708
BaeGI	GKGCMC	3 cut(s)	555, 2529, 2556
BalI	TGGCCA	1 cut(s)	1069
BamHI	GGATCC	2 cut(s)	383, 2584
BanI	GGYRCC	1 cut(s)	2610
BanII	GRGCYC	1 cut(s)	1883
BauI	CACGAG	2 cut(s)	2063, 2651
BbrPI	CACGTG	1 cut(s)	1298
BbsI	GAAGAC	7 cut(s)	11, 88, 775, 1329, 2151, 2565, 3394
Bbv12I	GWGCWC	5 cut(s)	555, 1883, 2529, 2556, 3316
BbvI	GCAGC	6 cut(s)	1041, 2051, 2439, 2827, 2856, 3160
BceAI	ACGGC	3 cut(s)	810, 1042, 1365
BciT130I	CCWGG	2 cut(s)	795, 2885
BciVI	GTATCC	1 cut(s)	1720
BclI	TGATCA	2 cut(s)	1114, 2830
BfaI	CTAG	4 cut(s)	267, 917, 1646, 1709
BfmI	CTRYAG	4 cut(s)	1254, 1289, 3015, 3217
BfoI	RGCGCY	1 cut(s)	2614
BfrI	CTTAAG	2 cut(s)	2320, 2420
BfuAI	ACCTGC	1 cut(s)	1093
BfuI	GTATCC	1 cut(s)	1720
BglII	AGATCT	1 cut(s)	451
BisI	GCNGC	6 cut(s)	1030, 2040, 2428, 2841, 2870, 3149
BlnI	CCTAGG	1 cut(s)	1708
BlsI	GCNGC	6 cut(s)	1031, 2041, 2429, 2842, 2871, 3150
Bme1390I	CCNGG	2 cut(s)	795, 2885
Bme18I	GGWCC	4 cut(s)	657, 884, 1642, 2881
BmgBI	CACGTC	1 cut(s)	1456
BmgT120I	GGNCC	4 cut(s)	657, 884, 1642, 2881
BmrFI	CCNGG	2 cut(s)	795, 2885
BmrI	ACTGGG	2 cut(s)	2012, 3001
BmuI	ACTGGG	2 cut(s)	2012, 3001
BpiI	GAAGAC	7 cut(s)	11, 88, 775, 1329, 2151, 2565, 3394
BpmI	CTGGAG	4 cut(s)	347, 1188, 2399, 2948
Bpu10I	CCTNAGC	1 cut(s)	298
BpuEI	CTTGAG	1 cut(s)	2935
BsaAI	YACGTR	1 cut(s)	1298
BsaBI	GATNNNNATC	1 cut(s)	3126
BsaHI	GRCGYC	1 cut(s)	2611
BsaI	GGTCTC	5 cut(s)	1206, 1643, 1923, 2528, 2632
BsaJI	CCNNGG	7 cut(s)	123, 793, 1637, 1708, 2640, 2721, 2763
BsaWI	WCCGGW	2 cut(s)	537, 2096
BsaXI	ACNNNNNCTCC	6 cut(s)	1946, 1976, 2732, 2762, 3166, 3196
Bsc4I	CCNNNNNNNGG	5 cut(s)	752, 1761, 2545, 2932, 2948
Bse118I	RCCGGY	1 cut(s)	2096
Bse1I	ACTGG	9 cut(s)	330, 470, 743, 929, 978, 1894, 2007, 2996, 3307
Bse3DI	GCAATG	1 cut(s)	3356
Bse8I	GATNNNNATC	1 cut(s)	3126
BseAI	TCCGGA	1 cut(s)	537
BseBI	CCWGG	2 cut(s)	795, 2885
BseDI	CCNNGG	7 cut(s)	123, 793, 1637, 1708, 2640, 2721, 2763
BseJI	GATNNNNATC	1 cut(s)	3126
BseLI	CCNNNNNNNGG	5 cut(s)	752, 1761, 2545, 2932, 2948
BseMI	GCAATG	1 cut(s)	3356
BseMII	CTCAG	9 cut(s)	1770, 1902, 1950, 2147, 2505, 2544, 2583, 2645, 3394
BseNI	ACTGG	9 cut(s)	330, 470, 743, 929, 978, 1894, 2007, 2996, 3307
BseRI	GAGGAG	1 cut(s)	2274
BseSI	GKGCMC	3 cut(s)	555, 2529, 2556
BseXI	GCAGC	6 cut(s)	1041, 2051, 2439, 2827, 2856, 3160
BseYI	CCCAGC	1 cut(s)	113
BsgI	GTGCAG	5 cut(s)	2058, 2113, 2446, 2684, 2940
Bsh1236I	CGCG	1 cut(s)	1639
BshFI	GGCC	3 cut(s)	296, 355, 1069
BshNI	GGYRCC	1 cut(s)	2610
BshTI	ACCGGT	1 cut(s)	2096
BsiHKAI	GWGCWC	5 cut(s)	555, 1883, 2529, 2556, 3316
BsiSI	CCGG	5 cut(s)	23, 538, 1273, 2097, 2942
BslFI	GGGAC	3 cut(s)	670, 897, 2706
BslI	CCNNNNNNNGG	5 cut(s)	752, 1761, 2545, 2932, 2948
BsmBI	CGTCTC	1 cut(s)	469
BsmFI	GGGAC	3 cut(s)	670, 897, 2706
BsmI	GAATGC	3 cut(s)	629, 3099, 3266
BsnI	GGCC	3 cut(s)	296, 355, 1069
Bso31I	GGTCTC	5 cut(s)	1206, 1643, 1923, 2528, 2632
Bsp1286I	GDGCHC	5 cut(s)	555, 1883, 2529, 2556, 3316
Bsp13I	TCCGGA	1 cut(s)	537
BspACI	CCGC	7 cut(s)	731, 1637, 1639, 1814, 2624, 3061, 3089
BspANI	GGCC	3 cut(s)	296, 355, 1069
BspCNI	CTCAG	9 cut(s)	1769, 1903, 1951, 2146, 2504, 2543, 2582, 2644, 3393
BspEI	TCCGGA	1 cut(s)	537
BspFNI	CGCG	1 cut(s)	1639
BspHI	TCATGA	1 cut(s)	1561
BspMI	ACCTGC	1 cut(s)	1093
BspPI	GGATC	9 cut(s)	187, 378, 391, 2351, 2579, 2592, 2703, 3008, 3175
BspQI	GCTCTTC	3 cut(s)	591, 1543, 2859
BspT107I	GGYRCC	1 cut(s)	2610
BspTI	CTTAAG	2 cut(s)	2320, 2420
BspTNI	GGTCTC	5 cut(s)	1206, 1643, 1923, 2528, 2632
BsrDI	GCAATG	1 cut(s)	3356
BsrFI	RCCGGY	1 cut(s)	2096
BsrI	ACTGG	9 cut(s)	330, 470, 743, 929, 978, 1894, 2007, 2996, 3307
BssAI	RCCGGY	1 cut(s)	2096
BssECI	CCNNGG	7 cut(s)	123, 793, 1637, 1708, 2640, 2721, 2763
BssNI	GRCGYC	1 cut(s)	2611
BssSI	CACGAG	2 cut(s)	2063, 2651
BssT1I	CCWWGG	5 cut(s)	123, 1708, 2640, 2721, 2763
Bst2BI	CACGAG	2 cut(s)	2063, 2651
Bst2UI	CCWGG	2 cut(s)	795, 2885
Bst6I	CTCTTC	6 cut(s)	591, 1097, 1290, 1543, 2300, 2859
BstACI	GRCGYC	1 cut(s)	2611
BstAFI	CTTAAG	2 cut(s)	2320, 2420
BstAPI	GCANNNNNTGC	1 cut(s)	1525
BstBAI	YACGTR	1 cut(s)	1298
BstC8I	GCNNGC	4 cut(s)	704, 2172, 2838, 3153
BstDSI	CCRYGG	1 cut(s)	1637
BstENI	CCTNNNNNAGG	1 cut(s)	1759
BstFNI	CGCG	1 cut(s)	1639
BstH2I	RGCGCY	1 cut(s)	2614
BstHHI	GCGC	1 cut(s)	2613
BstMWI	GCNNNNNNNGC	3 cut(s)	1525, 2894, 3411
BstNI	CCWGG	2 cut(s)	795, 2885
BstNSI	RCATGY	4 cut(s)	706, 819, 2174, 2840
BstSCI	CCNGG	2 cut(s)	793, 2883
BstSFI	CTRYAG	4 cut(s)	1254, 1289, 3015, 3217
BstSLI	GKGCMC	3 cut(s)	555, 2529, 2556
BstUI	CGCG	1 cut(s)	1639
BstV1I	GCAGC	6 cut(s)	1041, 2051, 2439, 2827, 2856, 3160
BstV2I	GAAGAC	7 cut(s)	11, 88, 775, 1329, 2151, 2565, 3394
BstX2I	RGATCY	4 cut(s)	383, 451, 2584, 3000
BstYI	RGATCY	4 cut(s)	383, 451, 2584, 3000
BsuI	GTATCC	1 cut(s)	1720
BsuRI	GGCC	3 cut(s)	296, 355, 1069
BtgI	CCRYGG	1 cut(s)	1637
BtrI	CACGTC	1 cut(s)	1456
BtsIMutI	CAGTG	6 cut(s)	477, 577, 1754, 2041, 3314, 3388
BveI	ACCTGC	1 cut(s)	1093
Cac8I	GCNNGC	4 cut(s)	704, 2172, 2838, 3153
CaiI	CAGNNNCTG	1 cut(s)	2369
CciI	TCATGA	1 cut(s)	1561
CfoI	GCGC	1 cut(s)	2613
Cfr10I	RCCGGY	1 cut(s)	2096
Cfr13I	GGNCC	4 cut(s)	657, 884, 1642, 2881
Cfr42I	CCGCGG	1 cut(s)	1640
CseI	GACGC	2 cut(s)	780, 868
Csp6I	GTAC	8 cut(s)	890, 1251, 1669, 1927, 1963, 2100, 2874, 2936
CspAI	ACCGGT	1 cut(s)	2096
CviQI	GTAC	8 cut(s)	890, 1251, 1669, 1927, 1963, 2100, 2874, 2936
DinI	GGCGCC	1 cut(s)	2612
DraI	TTTAAA	1 cut(s)	1159
DrdI	GACNNNNNNGTC	2 cut(s)	127, 2066
DseDI	GACNNNNNNGTC	2 cut(s)	127, 2066
EaeI	YGGCCR	1 cut(s)	1067
Eam1104I	CTCTTC	6 cut(s)	591, 1097, 1290, 1543, 2300, 2859
EarI	CTCTTC	6 cut(s)	591, 1097, 1290, 1543, 2300, 2859
Ecl136II	GAGCTC	1 cut(s)	1881
Eco130I	CCWWGG	5 cut(s)	123, 1708, 2640, 2721, 2763
Eco147I	AGGCCT	2 cut(s)	296, 355
Eco24I	GRGCYC	1 cut(s)	1883
Eco31I	GGTCTC	5 cut(s)	1206, 1643, 1923, 2528, 2632
Eco32I	GATATC	2 cut(s)	316, 583
Eco47I	GGWCC	4 cut(s)	657, 884, 1642, 2881
Eco53kI	GAGCTC	1 cut(s)	1881
Eco57I	CTGAAG	6 cut(s)	553, 609, 1121, 2548, 3128, 3305
Eco72I	CACGTG	1 cut(s)	1298
EcoICRI	GAGCTC	1 cut(s)	1881
EcoNI	CCTNNNNNAGG	1 cut(s)	1759
EcoO109I	RGGNCCY	3 cut(s)	884, 1642, 2881
EcoRI	GAATTC	4 cut(s)	836, 939, 1980, 2853
EcoRII	CCWGG	2 cut(s)	793, 2883
EcoRV	GATATC	2 cut(s)	316, 583
EcoT14I	CCWWGG	5 cut(s)	123, 1708, 2640, 2721, 2763
EcoT22I	ATGCAT	3 cut(s)	2407, 2418, 3101
EcoT38I	GRGCYC	1 cut(s)	1883
EgeI	GGCGCC	1 cut(s)	2612
EheI	GGCGCC	1 cut(s)	2612
ErhI	CCWWGG	5 cut(s)	123, 1708, 2640, 2721, 2763
Esp3I	CGTCTC	1 cut(s)	469
FalI	AAGNNNNNCTT	2 cut(s)	1508, 1540
FaqI	GGGAC	3 cut(s)	670, 897, 2706
FauI	CCCGC	1 cut(s)	1632
FbaI	TGATCA	2 cut(s)	1114, 2830
FblI	GTMKAC	1 cut(s)	394
Fnu4HI	GCNGC	6 cut(s)	1030, 2040, 2428, 2841, 2870, 3149
FriOI	GRGCYC	1 cut(s)	1883
Fsp4HI	GCNGC	6 cut(s)	1030, 2040, 2428, 2841, 2870, 3149
FspBI	CTAG	4 cut(s)	267, 917, 1646, 1709
GlaI	GCGC	1 cut(s)	2612
GluI	GCNGC	6 cut(s)	1030, 2040, 2428, 2841, 2870, 3149
GsaI	CCCAGC	1 cut(s)	117
GsuI	CTGGAG	4 cut(s)	347, 1188, 2399, 2948
HaeII	RGCGCY	1 cut(s)	2614
HaeIII	GGCC	3 cut(s)	296, 355, 1069
HapII	CCGG	5 cut(s)	23, 538, 1273, 2097, 2942
HgaI	GACGC	2 cut(s)	780, 868
HhaI	GCGC	1 cut(s)	2613
Hin1I	GRCGYC	1 cut(s)	2611
Hin6I	GCGC	1 cut(s)	2611
HinP1I	GCGC	1 cut(s)	2611
HincII	GTYRAC	1 cut(s)	3027
HindII	GTYRAC	1 cut(s)	3027
HindIII	AAGCTT	1 cut(s)	1357
HpaII	CCGG	5 cut(s)	23, 538, 1273, 2097, 2942
HphI	GGTGA	4 cut(s)	241, 995, 1181, 2899
Hpy99I	CGWCG	2 cut(s)	439, 1604
HpyCH4IV	ACGT	6 cut(s)	255, 690, 864, 1297, 1455, 3029
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1525, 2894, 3411
HpySE526I	ACGT	6 cut(s)	255, 690, 864, 1297, 1455, 3029
Hsp92I	GRCGYC	1 cut(s)	2611
HspAI	GCGC	1 cut(s)	2611
KasI	GGCGCC	1 cut(s)	2610
Kpn2I	TCCGGA	1 cut(s)	537
Ksp22I	TGATCA	2 cut(s)	1114, 2830
KspI	CCGCGG	1 cut(s)	1640
LguI	GCTCTTC	3 cut(s)	591, 1543, 2859
LmnI	GCTCC	3 cut(s)	962, 1878, 2287
Lsp1109I	GCAGC	6 cut(s)	1041, 2051, 2439, 2827, 2856, 3160
MaeI	CTAG	4 cut(s)	267, 917, 1646, 1709
MaeII	ACGT	6 cut(s)	255, 690, 864, 1297, 1455, 3029
MfeI	CAATTG	1 cut(s)	1504
MflI	RGATCY	4 cut(s)	383, 451, 2584, 3000
MhlI	GDGCHC	5 cut(s)	555, 1883, 2529, 2556, 3316
MlsI	TGGCCA	1 cut(s)	1069
MluNI	TGGCCA	1 cut(s)	1069
Mly113I	GGCGCC	1 cut(s)	2611
MlyI	GAGTC	6 cut(s)	419, 1364, 1687, 1964, 2099, 3207
MmeI	TCCRAC	4 cut(s)	813, 1473, 1753, 2978
Mox20I	TGGCCA	1 cut(s)	1069
Mph1103I	ATGCAT	3 cut(s)	2407, 2418, 3101
MroI	TCCGGA	1 cut(s)	537
MroXI	GAANNNNTTC	2 cut(s)	30, 2853
MscI	TGGCCA	1 cut(s)	1069
MslI	CAYNNNNRTG	5 cut(s)	254, 558, 1307, 2175, 2619
Msp20I	TGGCCA	1 cut(s)	1069
MspA1I	CMGCKG	2 cut(s)	1639, 3061
MspCI	CTTAAG	2 cut(s)	2320, 2420
MspI	CCGG	5 cut(s)	23, 538, 1273, 2097, 2942
MspR9I	CCNGG	2 cut(s)	795, 2885
MunI	CAATTG	1 cut(s)	1504
Mva1269I	GAATGC	3 cut(s)	629, 3099, 3266
MvaI	CCWGG	2 cut(s)	795, 2885
MvnI	CGCG	1 cut(s)	1639
MwoI	GCNNNNNNNGC	3 cut(s)	1525, 2894, 3411
NarI	GGCGCC	1 cut(s)	2611
NmuCI	GTSAC	4 cut(s)	35, 1293, 1298, 2905
NsiI	ATGCAT	3 cut(s)	2407, 2418, 3101
NspI	RCATGY	4 cut(s)	706, 819, 2174, 2840
OliI	CACNNNNGTG	1 cut(s)	254
PaeI	GCATGC	3 cut(s)	706, 2174, 2840
PagI	TCATGA	1 cut(s)	1561
PceI	AGGCCT	2 cut(s)	296, 355
PciSI	GCTCTTC	3 cut(s)	591, 1543, 2859
PctI	GAATGC	3 cut(s)	629, 3099, 3266
PdmI	GAANNNNTTC	2 cut(s)	30, 2853
PflMI	CCANNNNNTGG	1 cut(s)	2932
PinAI	ACCGGT	1 cut(s)	2096
PkrI	GCNGC	6 cut(s)	1031, 2041, 2429, 2842, 2871, 3150
PleI	GAGTC	6 cut(s)	419, 1364, 1687, 1963, 2099, 3206
PluTI	GGCGCC	1 cut(s)	2614
PmaCI	CACGTG	1 cut(s)	1298
PmlI	CACGTG	1 cut(s)	1298
PpsI	GAGTC	6 cut(s)	419, 1364, 1687, 1963, 2099, 3206
Ppu21I	YACGTR	1 cut(s)	1298
PpuMI	RGGWCCY	3 cut(s)	884, 1642, 2881
Psp124BI	GAGCTC	1 cut(s)	1883
Psp1406I	AACGTT	1 cut(s)	690
Psp5II	RGGWCCY	3 cut(s)	884, 1642, 2881
Psp6I	CCWGG	2 cut(s)	793, 2883
PspCI	CACGTG	1 cut(s)	1298
PspFI	CCCAGC	1 cut(s)	113
PspGI	CCWGG	2 cut(s)	793, 2883
PspPI	GGNCC	4 cut(s)	657, 884, 1642, 2881
PspPPI	RGGWCCY	3 cut(s)	884, 1642, 2881
PstNI	CAGNNNCTG	1 cut(s)	2369
PsuI	RGATCY	4 cut(s)	383, 451, 2584, 3000
RsaI	GTAC	8 cut(s)	891, 1252, 1670, 1928, 1964, 2101, 2875, 2937
RsaNI	GTAC	8 cut(s)	890, 1251, 1669, 1927, 1963, 2100, 2874, 2936
RseI	CAYNNNNRTG	5 cut(s)	254, 558, 1307, 2175, 2619
SacI	GAGCTC	1 cut(s)	1883
SacII	CCGCGG	1 cut(s)	1640
SapI	GCTCTTC	3 cut(s)	591, 1543, 2859
SatI	GCNGC	6 cut(s)	1030, 2040, 2428, 2841, 2870, 3149
Sau96I	GGNCC	4 cut(s)	657, 884, 1642, 2881
SchI	GAGTC	6 cut(s)	419, 1364, 1687, 1964, 2099, 3207
ScrFI	CCNGG	2 cut(s)	795, 2885
SduI	GDGCHC	5 cut(s)	555, 1883, 2529, 2556, 3316
SfcI	CTRYAG	4 cut(s)	1254, 1289, 3015, 3217
SfoI	GGCGCC	1 cut(s)	2612
Sfr303I	CCGCGG	1 cut(s)	1640
SgrBI	CCGCGG	1 cut(s)	1640
SinI	GGWCC	4 cut(s)	657, 884, 1642, 2881
SmiMI	CAYNNNNRTG	5 cut(s)	254, 558, 1307, 2175, 2619
SmlI	CTYRAG	3 cut(s)	2320, 2420, 2914
SmoI	CTYRAG	3 cut(s)	2320, 2420, 2914
SphI	GCATGC	3 cut(s)	706, 2174, 2840
SseBI	AGGCCT	2 cut(s)	296, 355
SsiI	CCGC	7 cut(s)	731, 1637, 1639, 1814, 2624, 3061, 3089
SspDI	GGCGCC	1 cut(s)	2610
SspMI	CTAG	4 cut(s)	267, 917, 1646, 1709
SstI	GAGCTC	1 cut(s)	1883
StuI	AGGCCT	2 cut(s)	296, 355
StyD4I	CCNGG	2 cut(s)	793, 2883
StyI	CCWWGG	5 cut(s)	123, 1708, 2640, 2721, 2763
TaiI	ACGT	6 cut(s)	258, 693, 867, 1300, 1458, 3032
TaqII	GACCGA	1 cut(s)	674
TatI	WGTACW	2 cut(s)	889, 1926
TscAI	CASTG	6 cut(s)	477, 577, 1754, 2041, 3314, 3388
TseFI	GTSAC	4 cut(s)	35, 1293, 1298, 2905
TseI	GCWGC	6 cut(s)	1029, 2039, 2427, 2840, 2869, 3148
Tsp45I	GTSAC	4 cut(s)	35, 1293, 1298, 2905
TspGWI	ACGGA	4 cut(s)	764, 1134, 1588, 3180
TspRI	CASTG	6 cut(s)	477, 577, 1754, 2041, 3314, 3388
Van91I	CCANNNNNTGG	1 cut(s)	2932
Vha464I	CTTAAG	2 cut(s)	2320, 2420
VneI	GTGCAC	3 cut(s)	551, 2525, 2552
VpaK11BI	GGWCC	4 cut(s)	657, 884, 1642, 2881
XagI	CCTNNNNNAGG	1 cut(s)	1759
XceI	RCATGY	4 cut(s)	706, 819, 2174, 2840
XcmI	CCANNNNNNNNNTGG	2 cut(s)	508, 3314
XmaJI	CCTAGG	1 cut(s)	1708
XmiI	GTMKAC	1 cut(s)	394
XmnI	GAANNNNTTC	2 cut(s)	30, 2853
XspI	CTAG	4 cut(s)	267, 917, 1646, 1709
Zsp2I	ATGCAT	3 cut(s)	2407, 2418, 3101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.