AT4G38700

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006333
Physical Location & Seq
Reverse (-)
18076301 .. 18077369
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G38700.1

Sequence Viewer

Length: 573 bp
ATGAAGAGCACATTAATAATATTTTTCACACTCTGTTTGAGCATGGCCGTTATGGCGCGTCACGAATCCTACTATGGCAACACCAAACCGGCTAAACTGAATGAGGAGAAAGTGACACGTGTCAGATTTTATCTCCATGATACTCTCAGTGGCCAAAATCCTACCGCCGTTAGAATCGCACATGCAAATCTAACCGGTGGCTCAGCTTCGCCGGTAGGTTTTGGAAGCCTGTTTGTGATAGACGATCCGCTCACCGTCGGGCCGGAGAAACACTCGAAAGAGATCGGGAATGGTCAAGGGATGTATGTCTCCGGGTGCAAAGACTTGAGCAAATTCACGATCGTCATGTACGCTGATTTGGCTTTCACGGCAGGGAAGTTTAATGGAAGCTCGATCAGTATCTTCTCGAGGAATCCGGTGGCGGAAGAGGTCGGAGAGCGAGAAATCGCGATTGTTGGTGGCCGTGGGAAATTCAGAATGGCGAGAGGTTTTGTCAAAGTAAAAACAAATAAGATTGACATGAAGACTGGTGATGCTGTTCTCCGATATGATGCTACTGTTTATCATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

190

Amino Acids

20.82

Weight (kDa)

9.41

Isoelectric Point (pI)

27.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 39 - 187 1.3e-50 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 250
AccII CGCG 2 cut(s) 58, 449
AciI CCGC 3 cut(s) 165, 248, 422
AclWI GGATC 1 cut(s) 239
AcoI YGGCCR 3 cut(s) 45, 151, 460
AcsI RAATTY 2 cut(s) 332, 470
AcvI CACGTG 1 cut(s) 119
AfaI GTAC 1 cut(s) 350
AflIII ACRYGT 2 cut(s) 116, 118
AgeI ACCGGT 1 cut(s) 194
AluBI AGCT 2 cut(s) 206, 390
AluI AGCT 2 cut(s) 206, 390
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 1 cut(s) 313
AlwI GGATC 1 cut(s) 239
Ama87I CYCGRG 1 cut(s) 406
AoxI GGCC 4 cut(s) 45, 151, 260, 460
ApoI RAATTY 2 cut(s) 332, 470
AseI ATTAAT 1 cut(s) 14
AsiGI ACCGGT 1 cut(s) 194
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 260
AsuC2I CCSGG 1 cut(s) 313
AsuHPI GGTGA 2 cut(s) 244, 542
AvaI CYCGRG 1 cut(s) 406
BalI TGGCCA 1 cut(s) 153
BbrPI CACGTG 1 cut(s) 119
BbsI GAAGAC 1 cut(s) 530
Bbv12I GWGCWC 1 cut(s) 11
BceAI ACGGC 4 cut(s) 32, 152, 384, 447
BcnI CCSGG 1 cut(s) 313
BcoDI GTCTC 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 53
BlpI GCTNAGC 1 cut(s) 202
Bme1390I CCNGG 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 406
BmgT120I GGNCC 1 cut(s) 260
BmrFI CCNGG 1 cut(s) 313
BmsI GCATC 2 cut(s) 523, 541
BoxI GACNNNNGTC 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 530
BplI GAGNNNNNCTC 2 cut(s) 257, 289
Bpu1102I GCTNAGC 1 cut(s) 202
BpuEI CTTGAG 1 cut(s) 346
BpuMI CCSGG 1 cut(s) 313
BsaAI YACGTR 1 cut(s) 119
BsaBI GATNNNNATC 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 463
BsaWI WCCGGW 2 cut(s) 194, 415
Bse118I RCCGGY 3 cut(s) 88, 194, 211
Bse1I ACTGG 1 cut(s) 532
Bse8I GATNNNNATC 1 cut(s) 398
BseDI CCNNGG 1 cut(s) 463
BseGI GGATG 1 cut(s) 306
BseJI GATNNNNATC 1 cut(s) 398
BseMII CTCAG 2 cut(s) 160, 216
BseNI ACTGG 1 cut(s) 532
BseRI GAGGAG 1 cut(s) 119
Bsh1236I CGCG 2 cut(s) 58, 449
Bsh1285I CGRYCG 1 cut(s) 342
BshFI GGCC 4 cut(s) 47, 153, 262, 462
BshTI ACCGGT 1 cut(s) 194
BsiEI CGRYCG 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 6 cut(s) 89, 195, 212, 263, 312, 416
BsmAI GTCTC 1 cut(s) 313
BsnI GGCC 4 cut(s) 47, 153, 262, 462
BsoBI CYCGRG 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 4 cut(s) 244, 282, 339, 393
Bsp1720I GCTNAGC 1 cut(s) 202
Bsp68I TCGCGA 1 cut(s) 449
BspACI CCGC 3 cut(s) 165, 248, 422
BspANI GGCC 4 cut(s) 47, 153, 262, 462
BspCNI CTCAG 2 cut(s) 159, 215
BspFNI CGCG 2 cut(s) 58, 449
BspPI GGATC 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 250
BsrFI RCCGGY 3 cut(s) 88, 194, 211
BsrI ACTGG 1 cut(s) 532
BssAI RCCGGY 3 cut(s) 88, 194, 211
BssECI CCNNGG 1 cut(s) 463
BssMI GATC 4 cut(s) 244, 282, 339, 393
Bst4CI ACNGT 2 cut(s) 256, 559
Bst6I CTCTTC 1 cut(s) 420
BstBAI YACGTR 1 cut(s) 119
BstDEI CTNAG 2 cut(s) 146, 202
BstDSI CCRYGG 1 cut(s) 463
BstF5I GGATG 1 cut(s) 306
BstFNI CGCG 2 cut(s) 58, 449
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 4 cut(s) 247, 285, 342, 396
BstMAI GTCTC 1 cut(s) 313
BstMBI GATC 4 cut(s) 244, 282, 339, 393
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 3 cut(s) 53, 359, 368
BstNSI RCATGY 1 cut(s) 185
BstPAI GACNNNNGTC 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 311
BstUI CGCG 2 cut(s) 58, 449
BstV2I GAAGAC 1 cut(s) 530
BsuRI GGCC 4 cut(s) 47, 153, 262, 462
BtgI CCRYGG 1 cut(s) 463
BtsCI GGATG 1 cut(s) 306
BtsIMutI CAGTG 1 cut(s) 154
BtuMI TCGCGA 1 cut(s) 449
CfoI GCGC 1 cut(s) 58
Cfr10I RCCGGY 3 cut(s) 88, 194, 211
Cfr13I GGNCC 1 cut(s) 260
CseI GACGC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 349
CspAI ACCGGT 1 cut(s) 194
CviAII CATG 5 cut(s) 43, 137, 182, 346, 520
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 2 cut(s) 146, 202
DpnI GATC 4 cut(s) 246, 284, 341, 395
DpnII GATC 4 cut(s) 244, 282, 339, 393
EaeI YGGCCR 3 cut(s) 45, 151, 460
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
EciI GGCGGA 1 cut(s) 437
Eco72I CACGTG 1 cut(s) 119
Eco88I CYCGRG 1 cut(s) 406
FaeI CATG 5 cut(s) 46, 140, 185, 349, 523
FaiI YATR 9 cut(s) 44, 53, 75, 138, 183, 306, 347, 521, 549
FatI CATG 5 cut(s) 42, 136, 181, 345, 519
FokI GGATG 1 cut(s) 313
GlaI GCGC 1 cut(s) 57
HaeIII GGCC 4 cut(s) 47, 153, 262, 462
HapII CCGG 6 cut(s) 89, 195, 212, 263, 312, 416
HgaI GACGC 1 cut(s) 47
HhaI GCGC 1 cut(s) 58
Hin1II CATG 5 cut(s) 46, 140, 185, 349, 523
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HinfI GANTC 3 cut(s) 65, 174, 412
HpaII CCGG 6 cut(s) 89, 195, 212, 263, 312, 416
HphI GGTGA 2 cut(s) 244, 542
Hpy188I TCNGA 4 cut(s) 125, 434, 476, 545
Hpy188III TCNNGA 5 cut(s) 62, 286, 337, 406, 448
Hpy99I CGWCG 1 cut(s) 260
HpyCH4III ACNGT 2 cut(s) 256, 559
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 2 cut(s) 185, 318
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 359, 368
HpyF3I CTNAG 2 cut(s) 146, 202
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 5 cut(s) 46, 140, 185, 349, 523
HspAI GCGC 1 cut(s) 56
Kzo9I GATC 4 cut(s) 244, 282, 339, 393
LpnPI CCDG 9 cut(s) 102, 208, 225, 242, 276, 325, 357, 429, 513
LweI GCATC 2 cut(s) 523, 541
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 2 cut(s) 59, 112
MalI GATC 4 cut(s) 246, 284, 341, 395
MbiI CCGCTC 1 cut(s) 250
MboI GATC 4 cut(s) 244, 282, 339, 393
MboII GAAGA 4 cut(s) 16, 394, 437, 535
MhlI GDGCHC 1 cut(s) 11
MlsI TGGCCA 1 cut(s) 153
MluCI AATT 2 cut(s) 332, 470
MluNI TGGCCA 1 cut(s) 153
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 4 cut(s) 97, 402, 421, 479
Mox20I TGGCCA 1 cut(s) 153
MscI TGGCCA 1 cut(s) 153
MseI TTAA 3 cut(s) 14, 381, 571
Msp20I TGGCCA 1 cut(s) 153
MspI CCGG 6 cut(s) 89, 195, 212, 263, 312, 416
MspR9I CCNGG 1 cut(s) 313
MvnI CGCG 2 cut(s) 58, 449
MwoI GCNNNNNNNGC 3 cut(s) 53, 359, 368
NciI CCSGG 1 cut(s) 313
NdeII GATC 4 cut(s) 244, 282, 339, 393
NlaIII CATG 5 cut(s) 46, 140, 185, 349, 523
NmuCI GTSAC 2 cut(s) 59, 112
NruI TCGCGA 1 cut(s) 449
NspI RCATGY 1 cut(s) 185
PaeR7I CTCGAG 1 cut(s) 406
PfeI GAWTC 3 cut(s) 65, 174, 412
PinAI ACCGGT 1 cut(s) 194
Ple19I CGATCG 1 cut(s) 342
PmaCI CACGTG 1 cut(s) 119
PmlI CACGTG 1 cut(s) 119
Ppu21I YACGTR 1 cut(s) 119
PshAI GACNNNNGTC 1 cut(s) 119
PshBI ATTAAT 1 cut(s) 14
PspCI CACGTG 1 cut(s) 119
PspPI GGNCC 1 cut(s) 260
PvuI CGATCG 1 cut(s) 342
RruI TCGCGA 1 cut(s) 449
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SaqAI TTAA 3 cut(s) 14, 381, 571
Sau3AI GATC 4 cut(s) 244, 282, 339, 393
Sau96I GGNCC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 313
SduI GDGCHC 1 cut(s) 11
SetI ASST 6 cut(s) 121, 208, 220, 392, 432, 490
SfaNI GCATC 2 cut(s) 523, 541
Sfr274I CTCGAG 1 cut(s) 406
SlaI CTCGAG 1 cut(s) 406
SmlI CTYRAG 2 cut(s) 325, 406
SmoI CTYRAG 2 cut(s) 325, 406
Sse9I AATT 2 cut(s) 332, 470
SsiI CCGC 3 cut(s) 165, 248, 422
SspI AATATT 1 cut(s) 21
StyD4I CCNGG 1 cut(s) 311
TaaI ACNGT 2 cut(s) 256, 559
TaiI ACGT 1 cut(s) 121
TaqI TCGA 3 cut(s) 275, 392, 407
TasI AATT 2 cut(s) 332, 470
TfiI GAWTC 3 cut(s) 65, 174, 412
Tru1I TTAA 3 cut(s) 14, 381, 571
Tru9I TTAA 3 cut(s) 14, 381, 571
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 2 cut(s) 59, 112
Tsp45I GTSAC 2 cut(s) 59, 112
TspDTI ATGAA 2 cut(s) 17, 536
TspRI CASTG 1 cut(s) 154
VspI ATTAAT 1 cut(s) 14
XapI RAATTY 2 cut(s) 332, 470
XceI RCATGY 1 cut(s) 185
XhoI CTCGAG 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.