Rh6BG020300

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0006333
Physical Location & Seq
Reverse (-)
3056126 .. 3056797
672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG020300.1

Sequence Viewer

Length: 651 bp
ATGGAGGATGTATCTCTATATAAACTTAAACCTAATCCCCATCAGTCTCTCACAACATTCAAAACTAGAAGAAAGATGGAGCGCAGTCGTTTGATTTTTGGGTTAGCCTTTACTATCCTTCTAACCATCGTCACCACACCTTCGCACTGCAAATACCACTCAAAAACAGTCCCTGTAGACCGCCTCAAAGAGAAAGTCACGCAACTCCACTTCTACCTCTTCGACATCCTCAGCGGCACCAAACCGAGCGCCGTGGAGATCGCACGTCCGAACGTCACCACTGACAAGTCAGCCACCCCTTTCGGGAGCCTCTATGCCTTCGATGACCATCTGCGAGAGGGCCCCGAGACCAACTCATCGATAGTCGGGAATGCGAAGGGGCTCTACTTGTCCGCCAGCCAAGACCCAGCTAACTTTACCATCGTGATGTACGCAGATTTCGGTTTTACTACTGGTAAGTTTAAAGGGAGCTCATTCGGCGTGTTTTCAAGGAATCCAGTGGCTGAGTCGCTGGAGCGGGAGGTGGCTGTGGTTGGTGGGAGAGGGAAGTTTAGGCTAGCTAGAGGGTTTGCTAGGGTTAAGACTCATTACTTCAATGCTACTAATGGTGATGCCATTTTGGAGTATAAGGCAACCATCATTCACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

216

Amino Acids

24.1

Weight (kDa)

9.71

Isoelectric Point (pI)

32.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 66 - 213 3.6e-47 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 236
AccBSI CCGCTC 1 cut(s) 517
AccI GTMKAC 1 cut(s) 177
AciI CCGC 4 cut(s) 181, 234, 393, 517
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 1 cut(s) 303
AgsI TTSAA 3 cut(s) 61, 489, 595
AjiI CACGTC 1 cut(s) 266
AluBI AGCT 3 cut(s) 410, 471, 560
AluI AGCT 3 cut(s) 410, 471, 560
Alw21I GWGCWC 1 cut(s) 473
Alw26I GTCTC 2 cut(s) 51, 341
AlwNI CAGNNNCTG 2 cut(s) 173, 503
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 1 cut(s) 340
ApaI GGGCCC 1 cut(s) 344
ArsI GACNNNNNNTTYG 2 cut(s) 180, 212
AspLEI GCGC 2 cut(s) 84, 251
AspS9I GGNCC 2 cut(s) 340, 341
AsuHPI GGTGA 4 cut(s) 124, 268, 620, 635
AsuNHI GCTAGC 1 cut(s) 556
AvaI CYCGRG 1 cut(s) 344
BaeGI GKGCMC 1 cut(s) 344
BanI GGYRCC 1 cut(s) 236
BanII GRGCYC 3 cut(s) 344, 384, 473
BarI GAAGNNNNNNTAC 2 cut(s) 368, 400
Bbv12I GWGCWC 1 cut(s) 473
BbvCI CCTCAGC 1 cut(s) 230
BccI CCATC 6 cut(s) 48, 70, 134, 336, 428, 644
BceAI ACGGC 1 cut(s) 236
BcoDI GTCTC 2 cut(s) 51, 341
BfaI CTAG 4 cut(s) 66, 557, 561, 573
BfmI CTRYAG 1 cut(s) 174
BfoI RGCGCY 1 cut(s) 252
BisI GCNGC 1 cut(s) 235
BlsI GCNGC 1 cut(s) 236
BmeT110I CYCGRG 1 cut(s) 344
BmgBI CACGTC 1 cut(s) 266
BmgT120I GGNCC 2 cut(s) 340, 341
BmiI GGNNCC 4 cut(s) 238, 308, 342, 343
BmsI GCATC 1 cut(s) 601
BmtI GCTAGC 1 cut(s) 560
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpmI CTGGAG 1 cut(s) 533
Bpu10I CCTNAGC 1 cut(s) 230
Bsa29I ATCGAT 1 cut(s) 359
BsaBI GATNNNNATC 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 252
Bsc4I CCNNNNNNNGG 1 cut(s) 303
Bse1I ACTGG 2 cut(s) 457, 497
Bse8I GATNNNNATC 1 cut(s) 327
BseCI ATCGAT 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 2 cut(s) 13, 225
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 1 cut(s) 303
BseMII CTCAG 2 cut(s) 244, 495
BseNI ACTGG 2 cut(s) 457, 497
BseSI GKGCMC 1 cut(s) 344
BseYI CCCAGC 1 cut(s) 406
BshFI GGCC 1 cut(s) 342
BshNI GGYRCC 1 cut(s) 236
BshVI ATCGAT 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 473
BsiHKCI CYCGRG 1 cut(s) 344
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 303
BsmAI GTCTC 2 cut(s) 51, 341
BsmFI GGGAC 1 cut(s) 155
BsmI GAATGC 1 cut(s) 376
BsnI GGCC 1 cut(s) 342
Bso31I GGTCTC 1 cut(s) 341
BsoBI CYCGRG 1 cut(s) 344
Bsp120I GGGCCC 1 cut(s) 340
Bsp1286I GDGCHC 3 cut(s) 344, 384, 473
Bsp143I GATC 1 cut(s) 258
BspACI CCGC 4 cut(s) 181, 234, 393, 517
BspANI GGCC 1 cut(s) 342
BspCNI CTCAG 2 cut(s) 243, 496
BspDI ATCGAT 1 cut(s) 359
BspLI GGNNCC 4 cut(s) 238, 308, 342, 343
BspOI GCTAGC 1 cut(s) 560
BspT107I GGYRCC 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 341
BsrBI CCGCTC 1 cut(s) 517
BsrI ACTGG 2 cut(s) 457, 497
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 1 cut(s) 258
Bst4CI ACNGT 1 cut(s) 169
Bst6I CTCTTC 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 397, 558
BstDEI CTNAG 2 cut(s) 230, 504
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 2 cut(s) 13, 225
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 2 cut(s) 84, 251
BstKTI GATC 1 cut(s) 261
BstMAI GTCTC 2 cut(s) 51, 341
BstMBI GATC 1 cut(s) 258
BstMWI GCNNNNNNNGC 1 cut(s) 477
BstSFI CTRYAG 1 cut(s) 174
BstSLI GKGCMC 1 cut(s) 344
Bsu15I ATCGAT 1 cut(s) 359
BsuRI GGCC 1 cut(s) 342
BsuTUI ATCGAT 1 cut(s) 359
BtgI CCRYGG 1 cut(s) 252
BtrI CACGTC 1 cut(s) 266
BtsCI GGATG 2 cut(s) 13, 225
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 3 cut(s) 145, 279, 504
Cac8I GCNNGC 2 cut(s) 397, 558
CaiI CAGNNNCTG 2 cut(s) 173, 503
CfoI GCGC 2 cut(s) 84, 251
Cfr13I GGNCC 2 cut(s) 340, 341
ClaI ATCGAT 1 cut(s) 359
Csp6I GTAC 1 cut(s) 430
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 2 cut(s) 230, 504
DpnI GATC 1 cut(s) 260
DpnII GATC 1 cut(s) 258
DraI TTTAAA 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
EciI GGCGGA 1 cut(s) 382
Ecl136II GAGCTC 1 cut(s) 471
Eco24I GRGCYC 3 cut(s) 344, 384, 473
Eco31I GGTCTC 1 cut(s) 341
Eco53kI GAGCTC 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 344
EcoICRI GAGCTC 1 cut(s) 471
EcoO109I RGGNCCY 2 cut(s) 340, 341
EcoT38I GRGCYC 3 cut(s) 344, 384, 473
FaiI YATR 4 cut(s) 19, 21, 315, 627
FaqI GGGAC 1 cut(s) 155
FauI CCCGC 1 cut(s) 510
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 1 cut(s) 235
FokI GGATG 2 cut(s) 20, 212
FriOI GRGCYC 3 cut(s) 344, 384, 473
Fsp4HI GCNGC 1 cut(s) 235
FspBI CTAG 4 cut(s) 66, 557, 561, 573
GlaI GCGC 2 cut(s) 83, 250
GluI GCNGC 1 cut(s) 235
GsaI CCCAGC 1 cut(s) 410
GsuI CTGGAG 1 cut(s) 533
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 1 cut(s) 342
HhaI GCGC 2 cut(s) 84, 251
Hin6I GCGC 2 cut(s) 82, 249
HinP1I GCGC 2 cut(s) 82, 249
HinfI GANTC 3 cut(s) 493, 506, 583
HphI GGTGA 4 cut(s) 124, 268, 620, 635
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 1 cut(s) 270
Hpy188III TCNNGA 3 cut(s) 304, 367, 424
Hpy8I GTNNAC 1 cut(s) 178
HpyAV CCTTC 4 cut(s) 128, 150, 328, 370
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4IV ACGT 2 cut(s) 265, 273
HpyCH4V TGCA 1 cut(s) 150
HpyF10VI GCNNNNNNNGC 1 cut(s) 477
HpyF3I CTNAG 2 cut(s) 230, 504
HpySE526I ACGT 2 cut(s) 265, 273
HspAI GCGC 2 cut(s) 82, 249
Kzo9I GATC 1 cut(s) 258
LmnI GCTCC 4 cut(s) 79, 306, 468, 514
LpnPI CCDG 6 cut(s) 186, 409, 420, 438, 497, 510
LweI GCATC 1 cut(s) 601
MaeI CTAG 4 cut(s) 66, 557, 561, 573
MaeII ACGT 2 cut(s) 265, 273
MaeIII GTNAC 3 cut(s) 130, 196, 274
MalI GATC 1 cut(s) 260
MbiI CCGCTC 1 cut(s) 517
MboI GATC 1 cut(s) 258
MboII GAAGA 2 cut(s) 81, 211
MhlI GDGCHC 3 cut(s) 344, 384, 473
MlyI GAGTC 2 cut(s) 515, 577
MnlI CCTC 8 cut(s) 194, 227, 239, 320, 331, 514, 536, 557
MseI TTAA 3 cut(s) 27, 462, 579
MslI CAYNNNNRTG 1 cut(s) 425
MspA1I CMGCKG 1 cut(s) 234
Mva1269I GAATGC 1 cut(s) 376
MwoI GCNNNNNNNGC 1 cut(s) 477
NdeII GATC 1 cut(s) 258
NheI GCTAGC 1 cut(s) 556
NlaIV GGNNCC 4 cut(s) 238, 308, 342, 343
NmuCI GTSAC 3 cut(s) 130, 196, 274
PctI GAATGC 1 cut(s) 376
PfeI GAWTC 1 cut(s) 493
PkrI GCNGC 1 cut(s) 236
PleI GAGTC 2 cut(s) 514, 577
PpsI GAGTC 2 cut(s) 514, 577
Psp124BI GAGCTC 1 cut(s) 473
PspFI CCCAGC 1 cut(s) 406
PspN4I GGNNCC 4 cut(s) 238, 308, 342, 343
PspOMI GGGCCC 1 cut(s) 340
PspPI GGNCC 2 cut(s) 340, 341
PstNI CAGNNNCTG 2 cut(s) 173, 503
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
RseI CAYNNNNRTG 1 cut(s) 425
SacI GAGCTC 1 cut(s) 473
SaqAI TTAA 3 cut(s) 27, 462, 579
SatI GCNGC 1 cut(s) 235
Sau3AI GATC 1 cut(s) 258
Sau96I GGNCC 2 cut(s) 340, 341
SchI GAGTC 2 cut(s) 515, 577
SduI GDGCHC 3 cut(s) 344, 384, 473
SetI ASST 9 cut(s) 34, 142, 219, 268, 276, 412, 473, 525, 562
SfaNI GCATC 1 cut(s) 601
SfcI CTRYAG 1 cut(s) 174
SmiMI CAYNNNNRTG 1 cut(s) 425
SsiI CCGC 4 cut(s) 181, 234, 393, 517
SspMI CTAG 4 cut(s) 66, 557, 561, 573
SstI GAGCTC 1 cut(s) 473
TaaI ACNGT 1 cut(s) 169
TaiI ACGT 2 cut(s) 268, 276
TaqI TCGA 3 cut(s) 222, 321, 359
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 1 cut(s) 493
Tru1I TTAA 3 cut(s) 27, 462, 579
Tru9I TTAA 3 cut(s) 27, 462, 579
TscAI CASTG 3 cut(s) 152, 286, 504
TseFI GTSAC 3 cut(s) 130, 196, 274
Tsp45I GTSAC 3 cut(s) 130, 196, 274
TspRI CASTG 3 cut(s) 152, 286, 504
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 4 cut(s) 66, 557, 561, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.