Rw6G002090

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0006333
Physical Location & Seq
Reverse (-)
3303384 .. 3303962
579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002090.1

Sequence Viewer

Length: 579 bp
ATGAACTGCAGTGGTTTGGTTCTCGGATTACTGGCCTTGCTTCTTCTAGCCATGGCCACCAAACCAGCACACTGCAAATACTACTCCAAAACAATCCCCAACATCCACCGCAAAGAGAAGGTGACGCACCTCCACTTCTACTACTTCGACATCCTCAGCGGCACCAAACCCAGCGCGGTGGAAATCGCACGTCCCAGCCTCACCAGAAACGACACGTCGCCTACACCCTTCGGCAGCCTCACTGCCACCGATGACCCTCTCCGCGAAGGCCCAGAGACCAACTCAACGGTGATCGGGAATGCCAGGGGGATGTACTTGTCGGCTAGCCAATACGCAAGCCAGATGACTTTGATGATGTACAAAGATTTCGGTTTTACTGCGGGTAAGTTTAAGGGGAGCTCTTTCGGGGTGTTTTCGAGGAATCCGATTTGGGAGACGACGCCGCGTGAGGTGGCGGTGGTGGGCGGGAGAGGGAAGTTTAGGTTGGCTAGAGGGTTTGCTAAGCTTAGGACTCATTACTTAAATGTTAGTAATGGTGATGCCGTTGTCGAGTATAGAGTGACTCTCATCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

192

Amino Acids

21.28

Weight (kDa)

9.72

Isoelectric Point (pI)

30.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 41 - 189 1.5e-47 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccII CGCG 3 cut(s) 176, 264, 445
AciI CCGC 8 cut(s) 109, 159, 176, 262, 380, 443, 455, 465
AcoI YGGCCR 1 cut(s) 54
AcyI GRCGYC 1 cut(s) 440
AfaI GTAC 2 cut(s) 314, 359
AflIII ACRYGT 1 cut(s) 213
AjiI CACGTC 2 cut(s) 191, 216
AjnI CCWGG 1 cut(s) 302
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
AluBI AGCT 2 cut(s) 399, 505
AluI AGCT 2 cut(s) 399, 505
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 2 cut(s) 269, 428
AoxI GGCC 3 cut(s) 33, 54, 268
ApeKI GCWGC 1 cut(s) 234
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 1 cut(s) 269
AsuHPI GGTGA 4 cut(s) 133, 193, 301, 548
AsuNHI GCTAGC 1 cut(s) 323
BalI TGGCCA 1 cut(s) 56
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 401
Bbv12I GWGCWC 1 cut(s) 401
BbvCI CCTCAGC 1 cut(s) 155
BbvI GCAGC 1 cut(s) 246
BceAI ACGGC 1 cut(s) 527
BciT130I CCWGG 1 cut(s) 304
BcoDI GTCTC 2 cut(s) 269, 428
BfaI CTAG 3 cut(s) 47, 324, 489
BfmI CTRYAG 1 cut(s) 7
BisI GCNGC 3 cut(s) 160, 235, 443
BlpI GCTNAGC 1 cut(s) 501
BlsI GCNGC 3 cut(s) 161, 236, 444
Bme1390I CCNGG 1 cut(s) 304
BmgBI CACGTC 2 cut(s) 191, 216
BmgT120I GGNCC 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 1 cut(s) 304
BmsI GCATC 1 cut(s) 529
BmtI GCTAGC 1 cut(s) 327
BplI GAGNNNNNCTC 3 cut(s) 266, 298, 549
Bpu10I CCTNAGC 2 cut(s) 155, 506
Bpu1102I GCTNAGC 1 cut(s) 501
BsaHI GRCGYC 1 cut(s) 440
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 51, 303
Bse1I ACTGG 1 cut(s) 36
BseBI CCWGG 1 cut(s) 304
BseDI CCNNGG 2 cut(s) 51, 303
BseGI GGATG 4 cut(s) 102, 150, 315, 567
BseMII CTCAG 1 cut(s) 169
BseNI ACTGG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 246
BseYI CCCAGC 2 cut(s) 170, 194
Bsh1236I CGCG 3 cut(s) 176, 264, 445
BshFI GGCC 3 cut(s) 35, 56, 270
BshNI GGYRCC 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 401
BslFI GGGAC 1 cut(s) 177
BsmAI GTCTC 2 cut(s) 269, 428
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 177
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 3 cut(s) 35, 56, 270
Bso31I GGTCTC 1 cut(s) 269
Bsp1286I GDGCHC 1 cut(s) 401
Bsp1407I TGTACA 1 cut(s) 357
Bsp143I GATC 1 cut(s) 291
Bsp1720I GCTNAGC 1 cut(s) 501
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 8 cut(s) 109, 159, 176, 262, 380, 443, 455, 465
BspANI GGCC 3 cut(s) 35, 56, 270
BspCNI CTCAG 1 cut(s) 168
BspFNI CGCG 3 cut(s) 176, 264, 445
BspLI GGNNCC 1 cut(s) 163
BspMAI CTGCAG 1 cut(s) 11
BspOI GCTAGC 1 cut(s) 327
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 269
BsrGI TGTACA 1 cut(s) 357
BsrI ACTGG 1 cut(s) 36
BssECI CCNNGG 2 cut(s) 51, 303
BssMI GATC 1 cut(s) 291
BssNI GRCGYC 1 cut(s) 440
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 304
Bst4CI ACNGT 1 cut(s) 289
BstACI GRCGYC 1 cut(s) 440
BstAUI TGTACA 1 cut(s) 357
BstC8I GCNNGC 2 cut(s) 325, 337
BstDEI CTNAG 3 cut(s) 155, 501, 506
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 4 cut(s) 102, 150, 315, 567
BstFNI CGCG 3 cut(s) 176, 264, 445
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 1 cut(s) 294
BstMAI GTCTC 2 cut(s) 269, 428
BstMBI GATC 1 cut(s) 291
BstNI CCWGG 1 cut(s) 304
BstSCI CCNGG 1 cut(s) 302
BstSFI CTRYAG 1 cut(s) 7
BstUI CGCG 3 cut(s) 176, 264, 445
BstV1I GCAGC 1 cut(s) 246
BstXI CCANNNNNNTGG 1 cut(s) 178
BsuRI GGCC 3 cut(s) 35, 56, 270
BtgI CCRYGG 1 cut(s) 51
BtrI CACGTC 2 cut(s) 191, 216
BtsCI GGATG 4 cut(s) 102, 150, 315, 567
BtsI GCAGTG 3 cut(s) 16, 70, 240
BtsIMutI CAGTG 3 cut(s) 16, 70, 240
Cac8I GCNNGC 2 cut(s) 325, 337
CfoI GCGC 1 cut(s) 176
Cfr13I GGNCC 1 cut(s) 269
CseI GACGC 2 cut(s) 133, 448
Csp6I GTAC 2 cut(s) 313, 358
CviAII CATG 1 cut(s) 52
CviQI GTAC 2 cut(s) 313, 358
DdeI CTNAG 3 cut(s) 155, 501, 506
DpnI GATC 1 cut(s) 293
DpnII GATC 1 cut(s) 291
EaeI YGGCCR 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 399
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 401
Eco31I GGTCTC 1 cut(s) 269
Eco53kI GAGCTC 1 cut(s) 399
EcoICRI GAGCTC 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 302
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 1 cut(s) 401
ErhI CCWWGG 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 1 cut(s) 55
FaiI YATR 2 cut(s) 53, 555
FaqI GGGAC 1 cut(s) 177
FatI CATG 1 cut(s) 51
FauI CCCGC 2 cut(s) 373, 458
Fnu4HI GCNGC 3 cut(s) 160, 235, 443
FokI GGATG 4 cut(s) 89, 137, 322, 554
FriOI GRGCYC 1 cut(s) 401
Fsp4HI GCNGC 3 cut(s) 160, 235, 443
FspBI CTAG 3 cut(s) 47, 324, 489
GlaI GCGC 1 cut(s) 175
GluI GCNGC 3 cut(s) 160, 235, 443
GsaI CCCAGC 2 cut(s) 174, 198
HaeIII GGCC 3 cut(s) 35, 56, 270
HgaI GACGC 2 cut(s) 133, 448
HhaI GCGC 1 cut(s) 176
Hin1I GRCGYC 1 cut(s) 440
Hin1II CATG 1 cut(s) 55
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 3 cut(s) 421, 511, 562
HphI GGTGA 4 cut(s) 133, 193, 301, 548
Hpy188I TCNGA 2 cut(s) 26, 426
Hpy188III TCNNGA 1 cut(s) 295
Hpy99I CGWCG 2 cut(s) 220, 442
HpyAV CCTTC 3 cut(s) 112, 238, 260
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 2 cut(s) 190, 215
HpyCH4V TGCA 2 cut(s) 9, 75
HpyF3I CTNAG 3 cut(s) 155, 501, 506
HpySE526I ACGT 2 cut(s) 190, 215
Hsp92I GRCGYC 1 cut(s) 440
Hsp92II CATG 1 cut(s) 55
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 1 cut(s) 291
LmnI GCTCC 1 cut(s) 396
LpnPI CCDG 9 cut(s) 17, 78, 184, 208, 217, 285, 289, 316, 353
Lsp1109I GCAGC 1 cut(s) 246
LweI GCATC 1 cut(s) 529
MaeI CTAG 3 cut(s) 47, 324, 489
MaeII ACGT 2 cut(s) 190, 215
MaeIII GTNAC 2 cut(s) 121, 559
MalI GATC 1 cut(s) 293
MboI GATC 1 cut(s) 291
MboII GAAGA 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 401
MlsI TGGCCA 1 cut(s) 56
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 2 cut(s) 505, 556
MnlI CCTC 9 cut(s) 140, 164, 209, 248, 267, 411, 442, 464, 485
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MseI TTAA 2 cut(s) 390, 521
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 304
Mva1269I GAATGC 1 cut(s) 304
MvaI CCWGG 1 cut(s) 304
MvnI CGCG 3 cut(s) 176, 264, 445
NcoI CCATGG 1 cut(s) 51
NdeII GATC 1 cut(s) 291
NheI GCTAGC 1 cut(s) 323
NlaIII CATG 1 cut(s) 55
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 2 cut(s) 121, 559
PcsI WCGNNNNNNNCGW 1 cut(s) 422
PctI GAATGC 1 cut(s) 304
PfeI GAWTC 1 cut(s) 421
PkrI GCNGC 3 cut(s) 161, 236, 444
PleI GAGTC 2 cut(s) 505, 556
PpsI GAGTC 2 cut(s) 505, 556
Psp124BI GAGCTC 1 cut(s) 401
Psp6I CCWGG 1 cut(s) 302
PspFI CCCAGC 2 cut(s) 170, 194
PspGI CCWGG 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 1 cut(s) 269
PstI CTGCAG 1 cut(s) 11
RsaI GTAC 2 cut(s) 314, 359
RsaNI GTAC 2 cut(s) 313, 358
SacI GAGCTC 1 cut(s) 401
SaqAI TTAA 2 cut(s) 390, 521
SatI GCNGC 3 cut(s) 160, 235, 443
Sau3AI GATC 1 cut(s) 291
Sau96I GGNCC 1 cut(s) 269
SchI GAGTC 2 cut(s) 505, 556
ScrFI CCNGG 1 cut(s) 304
SduI GDGCHC 1 cut(s) 401
SetI ASST 8 cut(s) 123, 132, 193, 218, 401, 453, 485, 507
SfaNI GCATC 1 cut(s) 529
SfcI CTRYAG 1 cut(s) 7
SsiI CCGC 8 cut(s) 109, 159, 176, 262, 380, 443, 455, 465
SspMI CTAG 3 cut(s) 47, 324, 489
SstI GAGCTC 1 cut(s) 401
StyD4I CCNGG 1 cut(s) 302
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 2 cut(s) 193, 218
TaqI TCGA 3 cut(s) 147, 416, 549
TatI WGTACW 2 cut(s) 312, 357
TauI GCSGC 2 cut(s) 162, 445
TfiI GAWTC 1 cut(s) 421
Tru1I TTAA 2 cut(s) 390, 521
Tru9I TTAA 2 cut(s) 390, 521
TscAI CASTG 3 cut(s) 16, 77, 247
TseFI GTSAC 2 cut(s) 121, 559
TseI GCWGC 1 cut(s) 234
Tsp45I GTSAC 2 cut(s) 121, 559
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 16, 77, 247
XspI CTAG 3 cut(s) 47, 324, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.