Rmu_sc0011292.1_g000011

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011292.1
OG0006333
Physical Location & Seq
Forward (+)
37728 .. 38303
576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011292.1_g000011.1.cds

Sequence Viewer

Length: 576 bp
atggagcgcagtcgtttgatttttgggttagcctttactatccttctaaccatcgtcaccacaccttcgcactgcaaataccactcaaaaacagtccctgtagaccgcctcaaagagaaagtcacgcaactccacttctacctcttcgacatcctcagcggcaccaaaccgagcgccgtggagatcgcacatccgaacgtcaccactgacaagtcagccacccctttcgggagcctctatgccttcgatgaccatctgcgagagggacccgagaccaactcatcgatagtcgggaatgcgaaggggctctacttgtccgccagccaagacccagctaactttaccatcgtgatgtacgcagatttcggttttactactggtaagtttaaagggagctcattcggcgtgttttcaaggaatccagtggctgagtcgccggagcgggaggtggctgtggttggtgggagagggaagtttaggctagctagagggtttgctagggttaagactcattacttcaatgctactaatggtgatgccattttggagtataaggcaaccatcattcaccattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

191

Amino Acids

21.06

Weight (kDa)

9.32

Isoelectric Point (pI)

30.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 442
AccI GTMKAC 1 cut(s) 102
AciI CCGC 4 cut(s) 106, 159, 318, 442
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 228
AgsI TTSAA 2 cut(s) 414, 520
AluBI AGCT 3 cut(s) 335, 396, 485
AluI AGCT 3 cut(s) 335, 396, 485
Alw21I GWGCWC 1 cut(s) 398
Alw26I GTCTC 1 cut(s) 266
AlwNI CAGNNNCTG 2 cut(s) 98, 428
Ama87I CYCGRG 1 cut(s) 269
ArsI GACNNNNNNTTYG 2 cut(s) 105, 137
AspLEI GCGC 2 cut(s) 9, 176
AspS9I GGNCC 1 cut(s) 266
AsuHPI GGTGA 4 cut(s) 49, 193, 545, 560
AsuNHI GCTAGC 1 cut(s) 481
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 1 cut(s) 266
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 2 cut(s) 309, 398
BarI GAAGNNNNNNTAC 2 cut(s) 293, 325
Bbv12I GWGCWC 1 cut(s) 398
BbvCI CCTCAGC 1 cut(s) 155
BccI CCATC 4 cut(s) 59, 261, 353, 569
BceAI ACGGC 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 3 cut(s) 482, 486, 498
BfmI CTRYAG 1 cut(s) 99
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 1 cut(s) 160
BlsI GCNGC 1 cut(s) 161
Bme18I GGWCC 1 cut(s) 266
BmeT110I CYCGRG 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 4 cut(s) 163, 233, 267, 268
BmsI GCATC 1 cut(s) 526
BmtI GCTAGC 1 cut(s) 485
BplI GAGNNNNNCTC 2 cut(s) 263, 295
Bpu10I CCTNAGC 1 cut(s) 155
Bsa29I ATCGAT 1 cut(s) 284
BsaBI GATNNNNATC 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 177
Bsc4I CCNNNNNNNGG 1 cut(s) 228
Bse1I ACTGG 2 cut(s) 382, 422
Bse8I GATNNNNATC 1 cut(s) 252
BseCI ATCGAT 1 cut(s) 284
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 2 cut(s) 150, 190
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 169, 420
BseNI ACTGG 2 cut(s) 382, 422
BseYI CCCAGC 1 cut(s) 331
BshNI GGYRCC 1 cut(s) 161
BshVI ATCGAT 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 398
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 1 cut(s) 437
BslFI GGGAC 2 cut(s) 80, 279
BslI CCNNNNNNNGG 1 cut(s) 228
BsmAI GTCTC 1 cut(s) 266
BsmFI GGGAC 2 cut(s) 80, 279
BsmI GAATGC 1 cut(s) 301
Bso31I GGTCTC 1 cut(s) 266
BsoBI CYCGRG 1 cut(s) 269
Bsp1286I GDGCHC 2 cut(s) 309, 398
Bsp143I GATC 1 cut(s) 183
BspACI CCGC 4 cut(s) 106, 159, 318, 442
BspCNI CTCAG 2 cut(s) 168, 421
BspDI ATCGAT 1 cut(s) 284
BspLI GGNNCC 4 cut(s) 163, 233, 267, 268
BspOI GCTAGC 1 cut(s) 485
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 266
BsrBI CCGCTC 1 cut(s) 442
BsrI ACTGG 2 cut(s) 382, 422
BssECI CCNNGG 1 cut(s) 177
BssMI GATC 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 94
Bst6I CTCTTC 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 322, 483
BstDEI CTNAG 2 cut(s) 155, 429
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 2 cut(s) 150, 190
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 2 cut(s) 9, 176
BstKTI GATC 1 cut(s) 186
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 1 cut(s) 183
BstMWI GCNNNNNNNGC 1 cut(s) 402
BstSFI CTRYAG 1 cut(s) 99
Bsu15I ATCGAT 1 cut(s) 284
BsuTUI ATCGAT 1 cut(s) 284
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 2 cut(s) 150, 190
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 3 cut(s) 70, 204, 429
Cac8I GCNNGC 2 cut(s) 322, 483
CaiI CAGNNNCTG 2 cut(s) 98, 428
CfoI GCGC 2 cut(s) 9, 176
Cfr13I GGNCC 1 cut(s) 266
ClaI ATCGAT 1 cut(s) 284
Csp6I GTAC 1 cut(s) 355
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 2 cut(s) 155, 429
DpnI GATC 1 cut(s) 185
DpnII GATC 1 cut(s) 183
DraI TTTAAA 1 cut(s) 388
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
EciI GGCGGA 1 cut(s) 307
Ecl136II GAGCTC 1 cut(s) 396
Eco24I GRGCYC 2 cut(s) 309, 398
Eco31I GGTCTC 1 cut(s) 266
Eco47I GGWCC 1 cut(s) 266
Eco53kI GAGCTC 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 396
EcoO109I RGGNCCY 1 cut(s) 266
EcoT38I GRGCYC 2 cut(s) 309, 398
FaiI YATR 2 cut(s) 240, 552
FaqI GGGAC 2 cut(s) 80, 279
FauI CCCGC 1 cut(s) 435
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 160
FokI GGATG 2 cut(s) 137, 177
FriOI GRGCYC 2 cut(s) 309, 398
Fsp4HI GCNGC 1 cut(s) 160
FspBI CTAG 3 cut(s) 482, 486, 498
GlaI GCGC 2 cut(s) 8, 175
GluI GCNGC 1 cut(s) 160
GsaI CCCAGC 1 cut(s) 335
HaeII RGCGCY 1 cut(s) 177
HapII CCGG 1 cut(s) 437
HhaI GCGC 2 cut(s) 9, 176
Hin6I GCGC 2 cut(s) 7, 174
HinP1I GCGC 2 cut(s) 7, 174
HinfI GANTC 3 cut(s) 418, 431, 508
HpaII CCGG 1 cut(s) 437
HphI GGTGA 4 cut(s) 49, 193, 545, 560
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 1 cut(s) 195
Hpy188III TCNNGA 3 cut(s) 229, 292, 349
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 4 cut(s) 53, 75, 253, 295
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 1 cut(s) 75
HpyF10VI GCNNNNNNNGC 1 cut(s) 402
HpyF3I CTNAG 2 cut(s) 155, 429
HpySE526I ACGT 1 cut(s) 198
HspAI GCGC 2 cut(s) 7, 174
KflI GGGWCCC 1 cut(s) 266
Kzo9I GATC 1 cut(s) 183
LmnI GCTCC 4 cut(s) 4, 231, 393, 439
LpnPI CCDG 6 cut(s) 111, 334, 345, 363, 435, 450
LweI GCATC 1 cut(s) 526
MaeI CTAG 3 cut(s) 482, 486, 498
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 3 cut(s) 55, 121, 199
MalI GATC 1 cut(s) 185
MbiI CCGCTC 1 cut(s) 442
MboI GATC 1 cut(s) 183
MboII GAAGA 1 cut(s) 136
MhlI GDGCHC 2 cut(s) 309, 398
MlyI GAGTC 2 cut(s) 440, 502
MnlI CCTC 8 cut(s) 119, 152, 164, 245, 256, 439, 461, 482
MseI TTAA 2 cut(s) 387, 504
MslI CAYNNNNRTG 1 cut(s) 350
MspA1I CMGCKG 1 cut(s) 159
MspI CCGG 1 cut(s) 437
Mva1269I GAATGC 1 cut(s) 301
MwoI GCNNNNNNNGC 1 cut(s) 402
NdeII GATC 1 cut(s) 183
NheI GCTAGC 1 cut(s) 481
NlaIV GGNNCC 4 cut(s) 163, 233, 267, 268
NmuCI GTSAC 3 cut(s) 55, 121, 199
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 418
PkrI GCNGC 1 cut(s) 161
PleI GAGTC 2 cut(s) 439, 502
PpsI GAGTC 2 cut(s) 439, 502
PpuMI RGGWCCY 1 cut(s) 266
Psp124BI GAGCTC 1 cut(s) 398
Psp5II RGGWCCY 1 cut(s) 266
PspFI CCCAGC 1 cut(s) 331
PspN4I GGNNCC 4 cut(s) 163, 233, 267, 268
PspPI GGNCC 1 cut(s) 266
PspPPI RGGWCCY 1 cut(s) 266
PstNI CAGNNNCTG 2 cut(s) 98, 428
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
RseI CAYNNNNRTG 1 cut(s) 350
SacI GAGCTC 1 cut(s) 398
SaqAI TTAA 2 cut(s) 387, 504
SatI GCNGC 1 cut(s) 160
Sau3AI GATC 1 cut(s) 183
Sau96I GGNCC 1 cut(s) 266
SchI GAGTC 2 cut(s) 440, 502
SduI GDGCHC 2 cut(s) 309, 398
SetI ASST 7 cut(s) 67, 144, 201, 337, 398, 450, 487
SfaNI GCATC 1 cut(s) 526
SfcI CTRYAG 1 cut(s) 99
SinI GGWCC 1 cut(s) 266
SmiMI CAYNNNNRTG 1 cut(s) 350
SsiI CCGC 4 cut(s) 106, 159, 318, 442
SspMI CTAG 3 cut(s) 482, 486, 498
SstI GAGCTC 1 cut(s) 398
TaaI ACNGT 1 cut(s) 94
TaiI ACGT 1 cut(s) 201
TaqI TCGA 3 cut(s) 147, 246, 284
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 1 cut(s) 418
Tru1I TTAA 2 cut(s) 387, 504
Tru9I TTAA 2 cut(s) 387, 504
TscAI CASTG 3 cut(s) 77, 211, 429
TseFI GTSAC 3 cut(s) 55, 121, 199
Tsp45I GTSAC 3 cut(s) 55, 121, 199
TspRI CASTG 3 cut(s) 77, 211, 429
VpaK11BI GGWCC 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 3 cut(s) 482, 486, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.