Rh6BG020400

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0006333
Physical Location & Seq
Reverse (-)
3059334 .. 3059861
528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG020400.1

Sequence Viewer

Length: 528 bp
ATGGCCTCCAAACCAGCACACTGCAAATACTACTCCAAAACAATCCCCAACATCCACCGCAAAGAGAAGGTGACGCACCTCCACTTCTACTACTTCGACATCCTCAGCGGCACCAAACCCAGCGCGGTGGAAATCGCACGTCCCAGCCTCACCAGAAACGACACGTCGCCTACACCCTTCGGCAGCCTCACTGCCACCGATGACCCTCTCCGCGAAGGCCCAGAGACCAACTCAACGGTGATCGGGAATGCCAGGGGGATGTACTTGTCGGCTAGCCAATACGCAAGCCAGATGACTTTGATGATGTACAAAGATTTCGGTTTTACTGCGGGTAAGTTTAAGGGGAGCTCTTTCGGGGTGTTTTCGAGGAATCCGATTTGGGAGACGACGCCGCGTGAGGTGGCGGTGGTGGGCGGGAGAGGGAAGTTTAGGTTGGCTAGAGGGTTTGCTAAGCTTAGGACTCATTACTTAAATGTTAGTAATGGTGATGCCGTTGTCGAGTATAGAGTGACTCTCATCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

175

Amino Acids

19.57

Weight (kDa)

9.79

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 24 - 172 1.1e-47 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 110
AccII CGCG 3 cut(s) 125, 213, 394
AciI CCGC 8 cut(s) 58, 108, 125, 211, 329, 392, 404, 414
AcyI GRCGYC 1 cut(s) 389
AfaI GTAC 2 cut(s) 263, 308
AflIII ACRYGT 1 cut(s) 162
AjiI CACGTC 2 cut(s) 140, 165
AjnI CCWGG 1 cut(s) 251
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AluBI AGCT 2 cut(s) 348, 454
AluI AGCT 2 cut(s) 348, 454
Alw21I GWGCWC 1 cut(s) 350
Alw26I GTCTC 2 cut(s) 218, 377
AoxI GGCC 2 cut(s) 3, 217
ApeKI GCWGC 1 cut(s) 183
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 218
AsuHPI GGTGA 4 cut(s) 82, 142, 250, 497
AsuNHI GCTAGC 1 cut(s) 272
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 350
BbvCI CCTCAGC 1 cut(s) 104
BbvI GCAGC 1 cut(s) 195
BceAI ACGGC 1 cut(s) 476
BciT130I CCWGG 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 218, 377
BfaI CTAG 2 cut(s) 273, 438
BisI GCNGC 3 cut(s) 109, 184, 392
BlpI GCTNAGC 1 cut(s) 450
BlsI GCNGC 3 cut(s) 110, 185, 393
Bme1390I CCNGG 1 cut(s) 253
BmgBI CACGTC 2 cut(s) 140, 165
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 112
BmrFI CCNGG 1 cut(s) 253
BmsI GCATC 1 cut(s) 478
BmtI GCTAGC 1 cut(s) 276
BplI GAGNNNNNCTC 3 cut(s) 215, 247, 498
Bpu10I CCTNAGC 2 cut(s) 104, 455
Bpu1102I GCTNAGC 1 cut(s) 450
BsaHI GRCGYC 1 cut(s) 389
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 252
BseBI CCWGG 1 cut(s) 253
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 4 cut(s) 51, 99, 264, 516
BseMII CTCAG 1 cut(s) 118
BseXI GCAGC 1 cut(s) 195
BseYI CCCAGC 2 cut(s) 119, 143
Bsh1236I CGCG 3 cut(s) 125, 213, 394
BshFI GGCC 2 cut(s) 5, 219
BshNI GGYRCC 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 350
BslFI GGGAC 1 cut(s) 126
BsmAI GTCTC 2 cut(s) 218, 377
BsmBI CGTCTC 1 cut(s) 377
BsmFI GGGAC 1 cut(s) 126
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 2 cut(s) 5, 219
Bso31I GGTCTC 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 350
Bsp1407I TGTACA 1 cut(s) 306
Bsp143I GATC 1 cut(s) 240
Bsp1720I GCTNAGC 1 cut(s) 450
BspACI CCGC 8 cut(s) 58, 108, 125, 211, 329, 392, 404, 414
BspANI GGCC 2 cut(s) 5, 219
BspCNI CTCAG 1 cut(s) 117
BspFNI CGCG 3 cut(s) 125, 213, 394
BspLI GGNNCC 1 cut(s) 112
BspOI GCTAGC 1 cut(s) 276
BspT107I GGYRCC 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 218
BsrGI TGTACA 1 cut(s) 306
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 1 cut(s) 240
BssNI GRCGYC 1 cut(s) 389
Bst2UI CCWGG 1 cut(s) 253
Bst4CI ACNGT 1 cut(s) 238
BstACI GRCGYC 1 cut(s) 389
BstAUI TGTACA 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 274, 286
BstDEI CTNAG 3 cut(s) 104, 450, 455
BstF5I GGATG 4 cut(s) 51, 99, 264, 516
BstFNI CGCG 3 cut(s) 125, 213, 394
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 1 cut(s) 243
BstMAI GTCTC 2 cut(s) 218, 377
BstMBI GATC 1 cut(s) 240
BstNI CCWGG 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 251
BstUI CGCG 3 cut(s) 125, 213, 394
BstV1I GCAGC 1 cut(s) 195
BstXI CCANNNNNNTGG 1 cut(s) 127
BsuRI GGCC 2 cut(s) 5, 219
BtrI CACGTC 2 cut(s) 140, 165
BtsCI GGATG 4 cut(s) 51, 99, 264, 516
BtsI GCAGTG 2 cut(s) 19, 189
BtsIMutI CAGTG 2 cut(s) 19, 189
Cac8I GCNNGC 2 cut(s) 274, 286
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 218
CseI GACGC 2 cut(s) 82, 397
Csp6I GTAC 2 cut(s) 262, 307
CviQI GTAC 2 cut(s) 262, 307
DdeI CTNAG 3 cut(s) 104, 450, 455
DpnI GATC 1 cut(s) 242
DpnII GATC 1 cut(s) 240
Ecl136II GAGCTC 1 cut(s) 348
Eco24I GRGCYC 1 cut(s) 350
Eco31I GGTCTC 1 cut(s) 218
Eco53kI GAGCTC 1 cut(s) 348
EcoICRI GAGCTC 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 251
EcoT38I GRGCYC 1 cut(s) 350
Esp3I CGTCTC 1 cut(s) 377
FaiI YATR 1 cut(s) 504
FaqI GGGAC 1 cut(s) 126
FauI CCCGC 2 cut(s) 322, 407
Fnu4HI GCNGC 3 cut(s) 109, 184, 392
FokI GGATG 4 cut(s) 38, 86, 271, 503
FriOI GRGCYC 1 cut(s) 350
Fsp4HI GCNGC 3 cut(s) 109, 184, 392
FspBI CTAG 2 cut(s) 273, 438
GlaI GCGC 1 cut(s) 124
GluI GCNGC 3 cut(s) 109, 184, 392
GsaI CCCAGC 2 cut(s) 123, 147
HaeIII GGCC 2 cut(s) 5, 219
HgaI GACGC 2 cut(s) 82, 397
HhaI GCGC 1 cut(s) 125
Hin1I GRCGYC 1 cut(s) 389
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 3 cut(s) 370, 460, 511
HphI GGTGA 4 cut(s) 82, 142, 250, 497
Hpy188I TCNGA 1 cut(s) 375
Hpy188III TCNNGA 1 cut(s) 244
Hpy99I CGWCG 2 cut(s) 169, 391
HpyAV CCTTC 3 cut(s) 61, 187, 209
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 2 cut(s) 139, 164
HpyCH4V TGCA 1 cut(s) 24
HpyF3I CTNAG 3 cut(s) 104, 450, 455
HpySE526I ACGT 2 cut(s) 139, 164
Hsp92I GRCGYC 1 cut(s) 389
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 1 cut(s) 240
LmnI GCTCC 1 cut(s) 345
LpnPI CCDG 8 cut(s) 27, 133, 157, 166, 234, 238, 265, 302
Lsp1109I GCAGC 1 cut(s) 195
LweI GCATC 1 cut(s) 478
MaeI CTAG 2 cut(s) 273, 438
MaeII ACGT 2 cut(s) 139, 164
MaeIII GTNAC 2 cut(s) 70, 508
MalI GATC 1 cut(s) 242
MboI GATC 1 cut(s) 240
MhlI GDGCHC 1 cut(s) 350
MlyI GAGTC 2 cut(s) 454, 505
MseI TTAA 2 cut(s) 339, 470
MspA1I CMGCKG 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 253
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 1 cut(s) 253
MvnI CGCG 3 cut(s) 125, 213, 394
NdeII GATC 1 cut(s) 240
NheI GCTAGC 1 cut(s) 272
NlaIV GGNNCC 1 cut(s) 112
NmuCI GTSAC 2 cut(s) 70, 508
PcsI WCGNNNNNNNCGW 1 cut(s) 371
PctI GAATGC 1 cut(s) 253
PfeI GAWTC 1 cut(s) 370
PkrI GCNGC 3 cut(s) 110, 185, 393
PleI GAGTC 2 cut(s) 454, 505
PpsI GAGTC 2 cut(s) 454, 505
Psp124BI GAGCTC 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 251
PspFI CCCAGC 2 cut(s) 119, 143
PspGI CCWGG 1 cut(s) 251
PspN4I GGNNCC 1 cut(s) 112
PspPI GGNCC 1 cut(s) 218
RsaI GTAC 2 cut(s) 263, 308
RsaNI GTAC 2 cut(s) 262, 307
SacI GAGCTC 1 cut(s) 350
SaqAI TTAA 2 cut(s) 339, 470
SatI GCNGC 3 cut(s) 109, 184, 392
Sau3AI GATC 1 cut(s) 240
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 2 cut(s) 454, 505
ScrFI CCNGG 1 cut(s) 253
SduI GDGCHC 1 cut(s) 350
SetI ASST 8 cut(s) 72, 81, 142, 167, 350, 402, 434, 456
SfaNI GCATC 1 cut(s) 478
SsiI CCGC 8 cut(s) 58, 108, 125, 211, 329, 392, 404, 414
SspMI CTAG 2 cut(s) 273, 438
SstI GAGCTC 1 cut(s) 350
StyD4I CCNGG 1 cut(s) 251
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 2 cut(s) 142, 167
TaqI TCGA 3 cut(s) 96, 365, 498
TatI WGTACW 2 cut(s) 261, 306
TauI GCSGC 2 cut(s) 111, 394
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 2 cut(s) 339, 470
Tru9I TTAA 2 cut(s) 339, 470
TscAI CASTG 2 cut(s) 26, 196
TseFI GTSAC 2 cut(s) 70, 508
TseI GCWGC 1 cut(s) 183
Tsp45I GTSAC 2 cut(s) 70, 508
TspRI CASTG 2 cut(s) 26, 196
XspI CTAG 2 cut(s) 273, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.