Prupe.8G162000_v2.0.a1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0006333
Physical Location & Seq
Forward (+)
16987695 .. 16988597
903 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G162000.1

Sequence Viewer

Length: 570 bp
ATGGAAGGCAGTCGATTCGTCCTCGTTTTGGCTTTGACTCTCACCGCGATCACACCAACACTCTCTACCTACCACTCCGCCACGCTCCTCAACGTCCGCCACAGAGAAAAAGTCACGCGCCTCCACTTCTACCTCTTCGACATCATCAGCGGCACCAAACCCAGCGCAGTAGAAGTCGCACGTCCGAAGGGCGCAAAATACGACAAGTCGGCGACGCCATTCGGCAGCGTGTGGGCCATCGACGACGCGCTCCGTGAGGGCCCCGAGTTCACTTCAAAAGTGGTCGGGAATGCGCAGGGGCTCTACTTGTCATCCGTCCAGGACGAGAACAGCTTAGGGCTAGTGATGTACGCTGATTTCGGTTTTACGACGGGTAAGTTTAACGGGAGCTCCTTCAGCGTGTTCTCGAGGAACCCGATCATGGAGCCGGGGGAGCGTGAGCTGGCAGTCGTGGGAGGGAGAGGGAGGTTTAGAATGGCGAAAGGTTTTGTCAAGGTGAAAACACAATTTTTGAATGTTACCAACGGTGACGCCATTCTTGAGTACAGGGTTACTCTAGTTCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

190

Amino Acids

20.84

Weight (kDa)

9.48

Isoelectric Point (pI)

19.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 294
AccB1I GGYRCC 1 cut(s) 152
AccII CGCG 3 cut(s) 47, 118, 248
AciI CCGC 4 cut(s) 45, 78, 97, 150
AcuI CTGAAG 1 cut(s) 379
AcyI GRCGYC 2 cut(s) 215, 531
AfaI GTAC 2 cut(s) 350, 545
AfiI CCNNNNNNNGG 2 cut(s) 28, 421
AgsI TTSAA 2 cut(s) 276, 514
AjiI CACGTC 1 cut(s) 182
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 3 cut(s) 333, 390, 442
AluI AGCT 3 cut(s) 333, 390, 442
Alw21I GWGCWC 1 cut(s) 392
Ama87I CYCGRG 2 cut(s) 263, 406
AoxI GGCC 2 cut(s) 234, 259
ApaI GGGCCC 1 cut(s) 263
ApeKI GCWGC 1 cut(s) 225
AspLEI GCGC 5 cut(s) 120, 167, 194, 250, 295
AspS9I GGNCC 3 cut(s) 234, 259, 260
AsuC2I CCSGG 1 cut(s) 429
AsuHPI GGTGA 3 cut(s) 34, 508, 539
AvaI CYCGRG 2 cut(s) 263, 406
BaeGI GKGCMC 1 cut(s) 263
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 3 cut(s) 263, 303, 392
Bbv12I GWGCWC 1 cut(s) 392
BbvI GCAGC 1 cut(s) 237
BccI CCATC 1 cut(s) 245
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 320
BcnI CCSGG 1 cut(s) 429
BfaI CTAG 2 cut(s) 341, 557
BisI GCNGC 2 cut(s) 151, 226
BlsI GCNGC 2 cut(s) 152, 227
Bme1390I CCNGG 2 cut(s) 320, 429
BmeT110I CYCGRG 2 cut(s) 263, 406
BmgBI CACGTC 1 cut(s) 182
BmgT120I GGNCC 3 cut(s) 234, 259, 260
BmiI GGNNCC 5 cut(s) 154, 261, 262, 413, 426
BmrFI CCNGG 2 cut(s) 320, 429
Bpu10I CCTNAGC 1 cut(s) 334
BpuEI CTTGAG 1 cut(s) 560
BpuMI CCSGG 1 cut(s) 429
BsaHI GRCGYC 2 cut(s) 215, 531
BsaJI CCNNGG 1 cut(s) 428
BsaXI ACNNNNNCTCC 2 cut(s) 374, 404
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 421
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 1 cut(s) 428
BseGI GGATG 1 cut(s) 311
BseLI CCNNNNNNNGG 2 cut(s) 28, 421
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 263
BseXI GCAGC 1 cut(s) 237
BseYI CCCAGC 1 cut(s) 161
Bsh1236I CGCG 3 cut(s) 47, 118, 248
BshFI GGCC 2 cut(s) 236, 261
BshNI GGYRCC 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 392
BsiHKCI CYCGRG 2 cut(s) 263, 406
BsiSI CCGG 1 cut(s) 428
BslI CCNNNNNNNGG 2 cut(s) 28, 421
BsmI GAATGC 1 cut(s) 295
BsnI GGCC 2 cut(s) 236, 261
BsoBI CYCGRG 2 cut(s) 263, 406
Bsp120I GGGCCC 1 cut(s) 259
Bsp1286I GDGCHC 3 cut(s) 263, 303, 392
Bsp143I GATC 2 cut(s) 48, 417
BspACI CCGC 4 cut(s) 45, 78, 97, 150
BspANI GGCC 2 cut(s) 236, 261
BspFNI CGCG 3 cut(s) 47, 118, 248
BspLI GGNNCC 5 cut(s) 154, 261, 262, 413, 426
BspT107I GGYRCC 1 cut(s) 152
BssECI CCNNGG 1 cut(s) 428
BssMI GATC 2 cut(s) 48, 417
BssNI GRCGYC 2 cut(s) 215, 531
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 1 cut(s) 527
Bst6I CTCTTC 1 cut(s) 140
BstACI GRCGYC 2 cut(s) 215, 531
BstC8I GCNNGC 1 cut(s) 444
BstDEI CTNAG 1 cut(s) 334
BstF5I GGATG 1 cut(s) 311
BstFNI CGCG 3 cut(s) 47, 118, 248
BstHHI GCGC 5 cut(s) 120, 167, 194, 250, 295
BstKTI GATC 2 cut(s) 51, 420
BstMBI GATC 2 cut(s) 48, 417
BstMWI GCNNNNNNNGC 2 cut(s) 396, 433
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 2 cut(s) 318, 427
BstSLI GKGCMC 1 cut(s) 263
BstUI CGCG 3 cut(s) 47, 118, 248
BstV1I GCAGC 1 cut(s) 237
BsuRI GGCC 2 cut(s) 236, 261
BtrI CACGTC 1 cut(s) 182
BtsCI GGATG 1 cut(s) 311
Cac8I GCNNGC 1 cut(s) 444
CfoI GCGC 5 cut(s) 120, 167, 194, 250, 295
Cfr13I GGNCC 3 cut(s) 234, 259, 260
CseI GACGC 3 cut(s) 223, 254, 539
Csp6I GTAC 2 cut(s) 349, 544
CviAII CATG 1 cut(s) 421
CviJI RGCY 9 cut(s) 32, 236, 261, 301, 333, 340, 390, 427, 442
CviKI_1 RGCY 9 cut(s) 32, 236, 261, 301, 333, 340, 390, 427, 442
CviQI GTAC 2 cut(s) 349, 544
DdeI CTNAG 1 cut(s) 334
DpnI GATC 2 cut(s) 50, 419
DpnII GATC 2 cut(s) 48, 417
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
EciI GGCGGA 2 cut(s) 67, 86
Ecl136II GAGCTC 1 cut(s) 390
Eco24I GRGCYC 3 cut(s) 263, 303, 392
Eco53kI GAGCTC 1 cut(s) 390
Eco57I CTGAAG 1 cut(s) 379
Eco88I CYCGRG 2 cut(s) 263, 406
EcoICRI GAGCTC 1 cut(s) 390
EcoO109I RGGNCCY 2 cut(s) 259, 260
EcoRII CCWGG 1 cut(s) 318
EcoT38I GRGCYC 3 cut(s) 263, 303, 392
FaeI CATG 1 cut(s) 424
FaiI YATR 1 cut(s) 422
FatI CATG 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 151, 226
FokI GGATG 1 cut(s) 298
FriOI GRGCYC 3 cut(s) 263, 303, 392
Fsp4HI GCNGC 2 cut(s) 151, 226
FspBI CTAG 2 cut(s) 341, 557
FspI TGCGCA 1 cut(s) 294
GlaI GCGC 5 cut(s) 119, 166, 193, 249, 294
GluI GCNGC 2 cut(s) 151, 226
GsaI CCCAGC 1 cut(s) 165
HaeIII GGCC 2 cut(s) 236, 261
HapII CCGG 1 cut(s) 428
HgaI GACGC 3 cut(s) 223, 254, 539
HhaI GCGC 5 cut(s) 120, 167, 194, 250, 295
Hin1I GRCGYC 2 cut(s) 215, 531
Hin1II CATG 1 cut(s) 424
Hin6I GCGC 5 cut(s) 118, 165, 192, 248, 293
HinP1I GCGC 5 cut(s) 118, 165, 192, 248, 293
HinfI GANTC 2 cut(s) 15, 37
HpaII CCGG 1 cut(s) 428
HphI GGTGA 3 cut(s) 34, 508, 539
Hpy166II GTNNAC 1 cut(s) 270
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 3 cut(s) 286, 406, 539
Hpy8I GTNNAC 1 cut(s) 270
Hpy99I CGWCG 4 cut(s) 217, 245, 248, 373
HpyAV CCTTC 2 cut(s) 181, 403
HpyCH4III ACNGT 1 cut(s) 527
HpyCH4IV ACGT 2 cut(s) 93, 181
HpyF10VI GCNNNNNNNGC 2 cut(s) 396, 433
HpyF3I CTNAG 1 cut(s) 334
HpySE526I ACGT 2 cut(s) 93, 181
Hsp92I GRCGYC 2 cut(s) 215, 531
Hsp92II CATG 1 cut(s) 424
HspAI GCGC 5 cut(s) 118, 165, 192, 248, 293
Kzo9I GATC 2 cut(s) 48, 417
LmnI GCTCC 6 cut(s) 90, 255, 387, 395, 424, 433
LpnPI CCDG 7 cut(s) 175, 281, 305, 332, 428, 441, 532
Lsp1109I GCAGC 1 cut(s) 237
MaeI CTAG 2 cut(s) 341, 557
MaeII ACGT 2 cut(s) 93, 181
MaeIII GTNAC 4 cut(s) 112, 517, 527, 550
MalI GATC 2 cut(s) 50, 419
MboI GATC 2 cut(s) 48, 417
MboII GAAGA 1 cut(s) 127
MhlI GDGCHC 3 cut(s) 263, 303, 392
MluCI AATT 1 cut(s) 506
MlyI GAGTC 1 cut(s) 31
MnlI CCTC 9 cut(s) 32, 98, 131, 143, 250, 402, 449, 455, 459
MseI TTAA 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 150
MspI CCGG 1 cut(s) 428
MspR9I CCNGG 2 cut(s) 320, 429
Mva1269I GAATGC 1 cut(s) 295
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 3 cut(s) 47, 118, 248
MwoI GCNNNNNNNGC 2 cut(s) 396, 433
NciI CCSGG 1 cut(s) 429
NdeII GATC 2 cut(s) 48, 417
NlaIII CATG 1 cut(s) 424
NlaIV GGNNCC 5 cut(s) 154, 261, 262, 413, 426
NmuCI GTSAC 2 cut(s) 112, 527
NsbI TGCGCA 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 406
PcsI WCGNNNNNNNCGW 1 cut(s) 413
PctI GAATGC 1 cut(s) 295
PfeI GAWTC 1 cut(s) 15
PfoI TCCNGGA 1 cut(s) 318
PkrI GCNGC 2 cut(s) 152, 227
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Psp124BI GAGCTC 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 318
PspFI CCCAGC 1 cut(s) 161
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 5 cut(s) 154, 261, 262, 413, 426
PspOMI GGGCCC 1 cut(s) 259
PspPI GGNCC 3 cut(s) 234, 259, 260
RsaI GTAC 2 cut(s) 350, 545
RsaNI GTAC 2 cut(s) 349, 544
SacI GAGCTC 1 cut(s) 392
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 2 cut(s) 151, 226
Sau3AI GATC 2 cut(s) 48, 417
Sau96I GGNCC 3 cut(s) 234, 259, 260
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 2 cut(s) 320, 429
SduI GDGCHC 3 cut(s) 263, 303, 392
Sfr274I CTCGAG 1 cut(s) 406
SlaI CTCGAG 1 cut(s) 406
SmlI CTYRAG 2 cut(s) 406, 539
SmoI CTYRAG 2 cut(s) 406, 539
Sse9I AATT 1 cut(s) 506
SsiI CCGC 4 cut(s) 45, 78, 97, 150
SspMI CTAG 2 cut(s) 341, 557
SstI GAGCTC 1 cut(s) 392
StyD4I CCNGG 2 cut(s) 318, 427
TaaI ACNGT 1 cut(s) 527
TaiI ACGT 2 cut(s) 96, 184
TaqI TCGA 4 cut(s) 13, 138, 240, 407
TasI AATT 1 cut(s) 506
TatI WGTACW 1 cut(s) 543
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 1 cut(s) 15
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseFI GTSAC 2 cut(s) 112, 527
TseI GCWGC 1 cut(s) 225
Tsp45I GTSAC 2 cut(s) 112, 527
TspDTI ATGAA 1 cut(s) 551
TspGWI ACGGA 2 cut(s) 242, 304
XhoI CTCGAG 1 cut(s) 406
XspI CTAG 2 cut(s) 341, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.