RLG00000015342

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006333
Physical Location & Seq
Forward (+)
64797488 .. 64798063
576 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015342

Sequence Viewer

Length: 576 bp
ATGGAGCGTAGTCGTTTCATTTTTGGGTTGGCCTTTACTATCCTTCTGACCATCGTCACCACACCCTCGCAATGCAAATACCATTCAAAAACAGTCCCTGTAGACCGCCTCAAAGAGAAAGTCACGGAATTCCACTTCTACCTCTTCGACATCCTCAGCGGCACCAAACCCAGCGCCGTGGAGATCGCACGTCCGAACGTCACCACTGACAAGTCAGCCACGCCTTTCGGCAGCCTCTACGCCTTCGATGATCCTCTTCGAGAGGGCCCCGAGACCAACTCATCGATAGTCGGGAATGCGAAGGGGCTCTACTTGTCCGCCAGCCAAGACCCAGCTAACTTTACCATCGTGATGTACGCAGATTTCGGTTTTACTACCGGTAAGTTTAAAGGGAGCTCATTTGGCGTGTTTTCAAGGAATCCAGTGGCTGAGTCGCCGAAGCGGGAGGTGGCTGTGGTTGGTGGGAGAGGGAAGTTTAGGCTAGCTAGAGGGTTTGCTAGGGTTAAGACTCATTACTTCAATGCTACTAATGGTGATGCCATTTTGGAGTATAAGGCAACCATCATTCACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

192

Amino Acids

21.13

Weight (kDa)

9.52

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 42 - 188 4.1e-48 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccI GTMKAC 1 cut(s) 102
AciI CCGC 4 cut(s) 106, 159, 318, 442
AclWI GGATC 1 cut(s) 245
AcsI RAATTY 1 cut(s) 128
AfaI GTAC 1 cut(s) 356
AgeI ACCGGT 1 cut(s) 377
AgsI TTSAA 3 cut(s) 87, 414, 520
AjiI CACGTC 1 cut(s) 191
AluBI AGCT 3 cut(s) 335, 396, 485
AluI AGCT 3 cut(s) 335, 396, 485
Alw21I GWGCWC 1 cut(s) 398
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 1 cut(s) 245
AlwNI CAGNNNCTG 2 cut(s) 98, 428
Ama87I CYCGRG 1 cut(s) 269
AoxI GGCC 2 cut(s) 30, 265
ApaI GGGCCC 1 cut(s) 269
ApeKI GCWGC 1 cut(s) 231
ApoI RAATTY 1 cut(s) 128
ArsI GACNNNNNNTTYG 2 cut(s) 105, 137
AsiGI ACCGGT 1 cut(s) 377
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 265, 266
AsuHPI GGTGA 3 cut(s) 49, 193, 545
AsuNHI GCTAGC 1 cut(s) 481
AvaI CYCGRG 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 269
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 3 cut(s) 269, 309, 398
BarI GAAGNNNNNNTAC 2 cut(s) 293, 325
Bbv12I GWGCWC 1 cut(s) 398
BbvCI CCTCAGC 1 cut(s) 155
BbvI GCAGC 1 cut(s) 243
BccI CCATC 3 cut(s) 59, 353, 569
BceAI ACGGC 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 3 cut(s) 482, 486, 498
BfmI CTRYAG 1 cut(s) 99
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 2 cut(s) 160, 232
BlsI GCNGC 2 cut(s) 161, 233
BmeT110I CYCGRG 1 cut(s) 269
BmgBI CACGTC 1 cut(s) 191
BmgT120I GGNCC 2 cut(s) 265, 266
BmiI GGNNCC 3 cut(s) 163, 267, 268
BmsI GCATC 1 cut(s) 526
BmtI GCTAGC 1 cut(s) 485
BoxI GACNNNNGTC 1 cut(s) 53
BplI GAGNNNNNCTC 2 cut(s) 263, 295
Bpu10I CCTNAGC 1 cut(s) 155
Bsa29I ATCGAT 1 cut(s) 284
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 177
BsaWI WCCGGW 1 cut(s) 377
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 422
Bse3DI GCAATG 1 cut(s) 77
BseCI ATCGAT 1 cut(s) 284
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 1 cut(s) 150
BseMI GCAATG 1 cut(s) 77
BseMII CTCAG 2 cut(s) 169, 420
BseNI ACTGG 1 cut(s) 422
BseSI GKGCMC 1 cut(s) 269
BseXI GCAGC 1 cut(s) 243
BseYI CCCAGC 2 cut(s) 170, 331
BshFI GGCC 2 cut(s) 32, 267
BshNI GGYRCC 1 cut(s) 161
BshTI ACCGGT 1 cut(s) 377
BshVI ATCGAT 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 398
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 1 cut(s) 378
BslFI GGGAC 1 cut(s) 80
BsmAI GTCTC 1 cut(s) 266
BsmFI GGGAC 1 cut(s) 80
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 2 cut(s) 32, 267
Bso31I GGTCTC 1 cut(s) 266
BsoBI CYCGRG 1 cut(s) 269
Bsp120I GGGCCC 1 cut(s) 265
Bsp1286I GDGCHC 3 cut(s) 269, 309, 398
Bsp143I GATC 2 cut(s) 183, 250
BspACI CCGC 4 cut(s) 106, 159, 318, 442
BspANI GGCC 2 cut(s) 32, 267
BspCNI CTCAG 2 cut(s) 168, 421
BspDI ATCGAT 1 cut(s) 284
BspLI GGNNCC 3 cut(s) 163, 267, 268
BspOI GCTAGC 1 cut(s) 485
BspPI GGATC 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 77
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 1 cut(s) 422
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 1 cut(s) 177
BssMI GATC 2 cut(s) 183, 250
Bst4CI ACNGT 1 cut(s) 94
Bst6I CTCTTC 2 cut(s) 149, 261
BstC8I GCNNGC 2 cut(s) 322, 483
BstDEI CTNAG 2 cut(s) 155, 429
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 2 cut(s) 186, 253
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 2 cut(s) 183, 250
BstMWI GCNNNNNNNGC 1 cut(s) 402
BstPAI GACNNNNGTC 1 cut(s) 53
BstSFI CTRYAG 1 cut(s) 99
BstSLI GKGCMC 1 cut(s) 269
BstV1I GCAGC 1 cut(s) 243
BstXI CCANNNNNNTGG 1 cut(s) 178
Bsu15I ATCGAT 1 cut(s) 284
BsuRI GGCC 2 cut(s) 32, 267
BsuTUI ATCGAT 1 cut(s) 284
BtgI CCRYGG 1 cut(s) 177
BtrI CACGTC 1 cut(s) 191
BtsCI GGATG 1 cut(s) 150
BtsIMutI CAGTG 2 cut(s) 204, 429
Cac8I GCNNGC 2 cut(s) 322, 483
CaiI CAGNNNCTG 2 cut(s) 98, 428
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 2 cut(s) 265, 266
ClaI ATCGAT 1 cut(s) 284
Csp6I GTAC 1 cut(s) 355
CspAI ACCGGT 1 cut(s) 377
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 2 cut(s) 155, 429
DpnI GATC 2 cut(s) 185, 252
DpnII GATC 2 cut(s) 183, 250
DraI TTTAAA 1 cut(s) 388
Eam1104I CTCTTC 2 cut(s) 149, 261
EarI CTCTTC 2 cut(s) 149, 261
EciI GGCGGA 1 cut(s) 307
Ecl136II GAGCTC 1 cut(s) 396
Eco24I GRGCYC 3 cut(s) 269, 309, 398
Eco31I GGTCTC 1 cut(s) 266
Eco53kI GAGCTC 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 396
EcoO109I RGGNCCY 2 cut(s) 265, 266
EcoRI GAATTC 1 cut(s) 128
EcoT38I GRGCYC 3 cut(s) 269, 309, 398
FaiI YATR 1 cut(s) 552
FaqI GGGAC 1 cut(s) 80
FauI CCCGC 1 cut(s) 435
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 2 cut(s) 160, 232
FokI GGATG 1 cut(s) 137
FriOI GRGCYC 3 cut(s) 269, 309, 398
Fsp4HI GCNGC 2 cut(s) 160, 232
FspBI CTAG 3 cut(s) 482, 486, 498
GlaI GCGC 1 cut(s) 175
GluI GCNGC 2 cut(s) 160, 232
GsaI CCCAGC 2 cut(s) 174, 335
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 2 cut(s) 32, 267
HapII CCGG 1 cut(s) 378
HhaI GCGC 1 cut(s) 176
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HinfI GANTC 3 cut(s) 418, 431, 508
HpaII CCGG 1 cut(s) 378
HphI GGTGA 3 cut(s) 49, 193, 545
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 2 cut(s) 48, 195
Hpy188III TCNNGA 3 cut(s) 260, 292, 349
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 3 cut(s) 53, 253, 295
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4IV ACGT 2 cut(s) 190, 198
HpyCH4V TGCA 1 cut(s) 75
HpyF10VI GCNNNNNNNGC 1 cut(s) 402
HpyF3I CTNAG 2 cut(s) 155, 429
HpySE526I ACGT 2 cut(s) 190, 198
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 2 cut(s) 183, 250
LmnI GCTCC 2 cut(s) 4, 393
LpnPI CCDG 6 cut(s) 111, 184, 334, 345, 391, 435
Lsp1109I GCAGC 1 cut(s) 243
LweI GCATC 1 cut(s) 526
MaeI CTAG 3 cut(s) 482, 486, 498
MaeII ACGT 2 cut(s) 190, 198
MaeIII GTNAC 3 cut(s) 55, 121, 199
MalI GATC 2 cut(s) 185, 252
MboI GATC 2 cut(s) 183, 250
MboII GAAGA 2 cut(s) 136, 248
MhlI GDGCHC 3 cut(s) 269, 309, 398
MluCI AATT 1 cut(s) 128
MlyI GAGTC 2 cut(s) 440, 502
MseI TTAA 2 cut(s) 387, 504
MslI CAYNNNNRTG 1 cut(s) 350
MspA1I CMGCKG 1 cut(s) 159
MspI CCGG 1 cut(s) 378
Mva1269I GAATGC 1 cut(s) 301
MwoI GCNNNNNNNGC 1 cut(s) 402
NdeII GATC 2 cut(s) 183, 250
NheI GCTAGC 1 cut(s) 481
NlaIV GGNNCC 3 cut(s) 163, 267, 268
NmuCI GTSAC 3 cut(s) 55, 121, 199
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 418
PinAI ACCGGT 1 cut(s) 377
PkrI GCNGC 2 cut(s) 161, 233
PleI GAGTC 2 cut(s) 439, 502
PpsI GAGTC 2 cut(s) 439, 502
PshAI GACNNNNGTC 1 cut(s) 53
Psp124BI GAGCTC 1 cut(s) 398
PspFI CCCAGC 2 cut(s) 170, 331
PspN4I GGNNCC 3 cut(s) 163, 267, 268
PspOMI GGGCCC 1 cut(s) 265
PspPI GGNCC 2 cut(s) 265, 266
PstNI CAGNNNCTG 2 cut(s) 98, 428
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
RseI CAYNNNNRTG 1 cut(s) 350
SacI GAGCTC 1 cut(s) 398
SaqAI TTAA 2 cut(s) 387, 504
SatI GCNGC 2 cut(s) 160, 232
Sau3AI GATC 2 cut(s) 183, 250
Sau96I GGNCC 2 cut(s) 265, 266
SchI GAGTC 2 cut(s) 440, 502
SduI GDGCHC 3 cut(s) 269, 309, 398
SetI ASST 7 cut(s) 144, 193, 201, 337, 398, 450, 487
SfaNI GCATC 1 cut(s) 526
SfcI CTRYAG 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 350
Sse9I AATT 1 cut(s) 128
SsiI CCGC 4 cut(s) 106, 159, 318, 442
SspMI CTAG 3 cut(s) 482, 486, 498
SstI GAGCTC 1 cut(s) 398
TaaI ACNGT 1 cut(s) 94
TaiI ACGT 2 cut(s) 193, 201
TaqI TCGA 4 cut(s) 147, 246, 259, 284
TasI AATT 1 cut(s) 128
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 1 cut(s) 418
Tru1I TTAA 2 cut(s) 387, 504
Tru9I TTAA 2 cut(s) 387, 504
TscAI CASTG 2 cut(s) 211, 429
TseFI GTSAC 3 cut(s) 55, 121, 199
TseI GCWGC 1 cut(s) 231
Tsp45I GTSAC 3 cut(s) 55, 121, 199
TspDTI ATGAA 1 cut(s) 7
TspGWI ACGGA 1 cut(s) 140
TspRI CASTG 2 cut(s) 211, 429
XapI RAATTY 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 3 cut(s) 482, 486, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.