Rw6G000030

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0006333
Physical Location & Seq
Forward (+)
73486 .. 73989
504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G000030.1

Sequence Viewer

Length: 504 bp
ATGGCCACCAAACCAGCACACTGCAAATACTACTCCAAAACAATCCCCAAAATCCACCGCAAAGAGAAGGTGACGCACCTCCACTTCTACTACTTCGACATCCTCAGCGGCACCAAACCCAGCCTTACCAGAAAAGACACCTCGCCTACACCCTTCGACAGCCTCACTGCCACCGATGACCCTCTCCGCGAAGGCCCGGAGACCAACTCAACGGCGATCGGGAATGCCAGGGGGATATACTTGTCGGCGAGCCAATACGAAAGCCAGATGGCTTTGATGATGTACAAAGATTTCGGTTTTACTGCGGGTAAGTTTACGGGGAGCTCTTTCGGGGTATTTTCGAGGAACCCGATTTGGGAGACGACGCCGCGTGAGGTGGCGGTGGTGGGCGGGAAAGGGAAGTTTAGGTTGGCTAGAGGGTTTGCTAAGCTTAGGACTCATTACTTAAATGTTAGTAATGGTGATGCCGTTGTCGAGTATAGAGTGACTCTCATCCATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

167

Amino Acids

18.77

Weight (kDa)

9.65

Isoelectric Point (pI)

27.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 24 - 164 3.5e-40 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 110
AccII CGCG 2 cut(s) 189, 370
AciI CCGC 7 cut(s) 58, 108, 187, 305, 368, 380, 390
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 365
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 1 cut(s) 355
AjnI CCWGG 1 cut(s) 227
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 2 cut(s) 324, 430
AluI AGCT 2 cut(s) 324, 430
Alw21I GWGCWC 1 cut(s) 326
Alw26I GTCTC 2 cut(s) 194, 353
AoxI GGCC 2 cut(s) 3, 193
AspS9I GGNCC 1 cut(s) 194
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 2 cut(s) 82, 473
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 326
BbvCI CCTCAGC 1 cut(s) 104
BccI CCATC 1 cut(s) 262
BceAI ACGGC 2 cut(s) 228, 452
BciT130I CCWGG 1 cut(s) 229
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 2 cut(s) 194, 353
BfaI CTAG 1 cut(s) 414
BisI GCNGC 2 cut(s) 109, 368
BlpI GCTNAGC 1 cut(s) 426
BlsI GCNGC 2 cut(s) 110, 369
Bme1390I CCNGG 2 cut(s) 197, 229
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 2 cut(s) 112, 347
BmrFI CCNGG 2 cut(s) 197, 229
BmsI GCATC 1 cut(s) 454
BplI GAGNNNNNCTC 3 cut(s) 191, 223, 474
Bpu10I CCTNAGC 2 cut(s) 104, 431
Bpu1102I GCTNAGC 1 cut(s) 426
BpuMI CCSGG 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 365
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 1 cut(s) 228
Bsc4I CCNNNNNNNGG 1 cut(s) 355
BseBI CCWGG 1 cut(s) 229
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 2 cut(s) 99, 492
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMII CTCAG 1 cut(s) 118
BseYI CCCAGC 1 cut(s) 119
Bsh1236I CGCG 2 cut(s) 189, 370
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 2 cut(s) 5, 195
BshNI GGYRCC 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 326
BsiSI CCGG 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 2 cut(s) 194, 353
BsmBI CGTCTC 1 cut(s) 353
BsmI GAATGC 1 cut(s) 229
BsnI GGCC 2 cut(s) 5, 195
Bso31I GGTCTC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 326
Bsp1407I TGTACA 1 cut(s) 282
Bsp143I GATC 1 cut(s) 216
Bsp1720I GCTNAGC 1 cut(s) 426
BspACI CCGC 7 cut(s) 58, 108, 187, 305, 368, 380, 390
BspANI GGCC 2 cut(s) 5, 195
BspCNI CTCAG 1 cut(s) 117
BspFNI CGCG 2 cut(s) 189, 370
BspLI GGNNCC 2 cut(s) 112, 347
BspT107I GGYRCC 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 194
BsrGI TGTACA 1 cut(s) 282
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 1 cut(s) 216
BssNI GRCGYC 1 cut(s) 365
Bst2UI CCWGG 1 cut(s) 229
BstACI GRCGYC 1 cut(s) 365
BstAUI TGTACA 1 cut(s) 282
BstC8I GCNNGC 1 cut(s) 250
BstDEI CTNAG 3 cut(s) 104, 426, 431
BstF5I GGATG 2 cut(s) 99, 492
BstFNI CGCG 2 cut(s) 189, 370
BstKTI GATC 1 cut(s) 219
BstMAI GTCTC 2 cut(s) 194, 353
BstMBI GATC 1 cut(s) 216
BstMCI CGRYCG 1 cut(s) 219
BstNI CCWGG 1 cut(s) 229
BstSCI CCNGG 2 cut(s) 195, 227
BstUI CGCG 2 cut(s) 189, 370
BsuRI GGCC 2 cut(s) 5, 195
BtsCI GGATG 2 cut(s) 99, 492
BtsI GCAGTG 2 cut(s) 19, 165
BtsIMutI CAGTG 2 cut(s) 19, 165
Cac8I GCNNGC 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 194
CseI GACGC 2 cut(s) 82, 373
Csp6I GTAC 1 cut(s) 283
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 3 cut(s) 104, 426, 431
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 324
Eco24I GRGCYC 1 cut(s) 326
Eco31I GGTCTC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 324
EcoICRI GAGCTC 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 227
EcoT38I GRGCYC 1 cut(s) 326
Esp3I CGTCTC 1 cut(s) 353
FaiI YATR 2 cut(s) 238, 480
FauI CCCGC 2 cut(s) 298, 383
Fnu4HI GCNGC 2 cut(s) 109, 368
FokI GGATG 2 cut(s) 86, 479
FriOI GRGCYC 1 cut(s) 326
Fsp4HI GCNGC 2 cut(s) 109, 368
FspBI CTAG 1 cut(s) 414
GluI GCNGC 2 cut(s) 109, 368
GsaI CCCAGC 1 cut(s) 123
HaeIII GGCC 2 cut(s) 5, 195
HapII CCGG 1 cut(s) 197
HgaI GACGC 2 cut(s) 82, 373
Hin1I GRCGYC 1 cut(s) 365
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 2 cut(s) 436, 487
HpaII CCGG 1 cut(s) 197
HphI GGTGA 2 cut(s) 82, 473
Hpy166II GTNNAC 1 cut(s) 315
Hpy188III TCNNGA 1 cut(s) 220
Hpy8I GTNNAC 1 cut(s) 315
Hpy99I CGWCG 1 cut(s) 367
HpyAV CCTTC 3 cut(s) 61, 163, 185
HpyCH4V TGCA 1 cut(s) 24
HpyF3I CTNAG 3 cut(s) 104, 426, 431
Hsp92I GRCGYC 1 cut(s) 365
Kzo9I GATC 1 cut(s) 216
LmnI GCTCC 1 cut(s) 321
LpnPI CCDG 7 cut(s) 27, 133, 142, 210, 214, 241, 278
LweI GCATC 1 cut(s) 454
MaeI CTAG 1 cut(s) 414
MaeIII GTNAC 2 cut(s) 70, 484
MalI GATC 1 cut(s) 218
MboI GATC 1 cut(s) 216
MhlI GDGCHC 1 cut(s) 326
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 430, 481
MnlI CCTC 8 cut(s) 89, 113, 151, 173, 192, 336, 367, 410
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 446, 502
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 108
MspI CCGG 1 cut(s) 197
MspR9I CCNGG 2 cut(s) 197, 229
Mva1269I GAATGC 1 cut(s) 229
MvaI CCWGG 1 cut(s) 229
MvnI CGCG 2 cut(s) 189, 370
NciI CCSGG 1 cut(s) 197
NdeII GATC 1 cut(s) 216
NlaIV GGNNCC 2 cut(s) 112, 347
NmuCI GTSAC 2 cut(s) 70, 484
PcsI WCGNNNNNNNCGW 1 cut(s) 347
PctI GAATGC 1 cut(s) 229
PkrI GCNGC 2 cut(s) 110, 369
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 2 cut(s) 430, 481
PpsI GAGTC 2 cut(s) 430, 481
Psp124BI GAGCTC 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 227
PspFI CCCAGC 1 cut(s) 119
PspGI CCWGG 1 cut(s) 227
PspN4I GGNNCC 2 cut(s) 112, 347
PspPI GGNCC 1 cut(s) 194
PvuI CGATCG 1 cut(s) 219
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
SacI GAGCTC 1 cut(s) 326
SaqAI TTAA 2 cut(s) 446, 502
SatI GCNGC 2 cut(s) 109, 368
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 1 cut(s) 194
SchI GAGTC 2 cut(s) 430, 481
ScrFI CCNGG 2 cut(s) 197, 229
SduI GDGCHC 1 cut(s) 326
SetI ASST 7 cut(s) 72, 81, 143, 326, 378, 410, 432
SfaNI GCATC 1 cut(s) 454
SsiI CCGC 7 cut(s) 58, 108, 187, 305, 368, 380, 390
SspMI CTAG 1 cut(s) 414
SstI GAGCTC 1 cut(s) 326
StyD4I CCNGG 2 cut(s) 195, 227
TaqI TCGA 4 cut(s) 96, 156, 341, 474
TatI WGTACW 1 cut(s) 282
TauI GCSGC 2 cut(s) 111, 370
Tru1I TTAA 2 cut(s) 446, 502
Tru9I TTAA 2 cut(s) 446, 502
TscAI CASTG 2 cut(s) 26, 172
TseFI GTSAC 2 cut(s) 70, 484
Tsp45I GTSAC 2 cut(s) 70, 484
TspRI CASTG 2 cut(s) 26, 172
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.