Rh6CG018700

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0006333
Physical Location & Seq
Reverse (-)
1858293 .. 1858874
582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG018700.1

Sequence Viewer

Length: 582 bp
ATGATGGAACGCAGTGGTTTGGTTCTCGGACTACTGGCCTTGCTTCTTCTAGCCATGGCCACCAAACCAGCACAATGCAAATACTACTCCAAAACAATGCCCAACATCCACCGCAAAGAGAAGGTGACGCACCTCCACTTCTACTACTTCGACATCCTCAGCGGAACCAAACCCAGCGCGGTGGAAATCGCACGTCCCAGCCTCACCAGAAACGACACATCGCCCACACCCTTCGGCAGCCTCACTGCCACCGATGACCCTCTCCGCGAAGGCCCGGAGTCCAACTCAACGGTGATCGGGAATGCCAGGGGGATATACTTGTCGGCGAGCCAAGACGCAAGTCAGATGACTTTGGTGATGTACAAAGATTTCGGATTTTCTGCGGGTAAGTTTAAGGGGAGCTCTTTCGGGGTGTTTTCTAGGAATCCGATTTGGGAGACGACGCCGCGTGAGGTGGCGGTGGTGGGCGGGAGAGGGAAGTTTAGGTTGGCTAGAGGGTTTGCTAAGCTTAGGACTCATTACTTAAATGTTAGTAATGGTGATGCCGTTGTCGAGTATAGAGTGACTCTCATCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

193

Amino Acids

21.36

Weight (kDa)

9.74

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 42 - 190 1.4e-47 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 179, 267, 448
AciI CCGC 8 cut(s) 112, 162, 179, 265, 383, 446, 458, 468
AcoI YGGCCR 1 cut(s) 57
AcyI GRCGYC 1 cut(s) 443
AfaI GTAC 1 cut(s) 362
AjiI CACGTC 1 cut(s) 194
AjnI CCWGG 1 cut(s) 305
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AluBI AGCT 2 cut(s) 402, 508
AluI AGCT 2 cut(s) 402, 508
Alw21I GWGCWC 1 cut(s) 404
Alw26I GTCTC 1 cut(s) 431
AoxI GGCC 3 cut(s) 36, 57, 271
ApeKI GCWGC 1 cut(s) 237
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 272
AsuC2I CCSGG 1 cut(s) 275
AsuHPI GGTGA 5 cut(s) 136, 196, 304, 367, 551
BalI TGGCCA 1 cut(s) 59
BanII GRGCYC 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 404
BbvCI CCTCAGC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 249
BceAI ACGGC 1 cut(s) 530
BciT130I CCWGG 1 cut(s) 307
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 1 cut(s) 431
BfaI CTAG 3 cut(s) 50, 420, 492
BisI GCNGC 2 cut(s) 238, 446
BlpI GCTNAGC 1 cut(s) 504
BlsI GCNGC 2 cut(s) 239, 447
Bme1390I CCNGG 2 cut(s) 275, 307
BmgBI CACGTC 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 2 cut(s) 275, 307
BmsI GCATC 1 cut(s) 532
BoxI GACNNNNGTC 1 cut(s) 339
BplI GAGNNNNNCTC 3 cut(s) 269, 301, 552
Bpu10I CCTNAGC 2 cut(s) 158, 509
Bpu1102I GCTNAGC 1 cut(s) 504
BpuMI CCSGG 1 cut(s) 275
BsaHI GRCGYC 1 cut(s) 443
BsaJI CCNNGG 2 cut(s) 54, 306
Bse1I ACTGG 1 cut(s) 39
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 2 cut(s) 54, 306
BseGI GGATG 3 cut(s) 105, 153, 570
BseMII CTCAG 1 cut(s) 172
BseNI ACTGG 1 cut(s) 39
BseXI GCAGC 1 cut(s) 249
BseYI CCCAGC 2 cut(s) 173, 197
Bsh1236I CGCG 3 cut(s) 179, 267, 448
BshFI GGCC 3 cut(s) 38, 59, 273
BsiHKAI GWGCWC 1 cut(s) 404
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 1 cut(s) 180
BsmAI GTCTC 1 cut(s) 431
BsmBI CGTCTC 1 cut(s) 431
BsmFI GGGAC 1 cut(s) 180
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 3 cut(s) 38, 59, 273
Bsp1286I GDGCHC 1 cut(s) 404
Bsp1407I TGTACA 1 cut(s) 360
Bsp143I GATC 1 cut(s) 294
Bsp1720I GCTNAGC 1 cut(s) 504
Bsp19I CCATGG 1 cut(s) 54
BspACI CCGC 8 cut(s) 112, 162, 179, 265, 383, 446, 458, 468
BspANI GGCC 3 cut(s) 38, 59, 273
BspCNI CTCAG 1 cut(s) 171
BspFNI CGCG 3 cut(s) 179, 267, 448
BspLI GGNNCC 1 cut(s) 166
BsrGI TGTACA 1 cut(s) 360
BsrI ACTGG 1 cut(s) 39
BssECI CCNNGG 2 cut(s) 54, 306
BssMI GATC 1 cut(s) 294
BssNI GRCGYC 1 cut(s) 443
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 307
Bst4CI ACNGT 1 cut(s) 292
BstACI GRCGYC 1 cut(s) 443
BstAUI TGTACA 1 cut(s) 360
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 3 cut(s) 158, 504, 509
BstDSI CCRYGG 1 cut(s) 54
BstF5I GGATG 3 cut(s) 105, 153, 570
BstFNI CGCG 3 cut(s) 179, 267, 448
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 1 cut(s) 297
BstMAI GTCTC 1 cut(s) 431
BstMBI GATC 1 cut(s) 294
BstNI CCWGG 1 cut(s) 307
BstPAI GACNNNNGTC 1 cut(s) 339
BstSCI CCNGG 2 cut(s) 273, 305
BstUI CGCG 3 cut(s) 179, 267, 448
BstV1I GCAGC 1 cut(s) 249
BstXI CCANNNNNNTGG 1 cut(s) 181
BsuRI GGCC 3 cut(s) 38, 59, 273
BtgI CCRYGG 1 cut(s) 54
BtgZI GCGATG 1 cut(s) 204
BtrI CACGTC 1 cut(s) 194
BtsCI GGATG 3 cut(s) 105, 153, 570
BtsI GCAGTG 2 cut(s) 19, 243
BtsIMutI CAGTG 2 cut(s) 19, 243
Cac8I GCNNGC 1 cut(s) 328
CfoI GCGC 1 cut(s) 179
Cfr13I GGNCC 1 cut(s) 272
CseI GACGC 3 cut(s) 136, 344, 451
Csp6I GTAC 1 cut(s) 361
CviAII CATG 1 cut(s) 55
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 3 cut(s) 158, 504, 509
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
EaeI YGGCCR 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 402
Eco130I CCWWGG 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 404
Eco53kI GAGCTC 1 cut(s) 402
EcoICRI GAGCTC 1 cut(s) 402
EcoRII CCWGG 1 cut(s) 305
EcoT14I CCWWGG 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 404
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 431
FaeI CATG 1 cut(s) 58
FaiI YATR 3 cut(s) 56, 316, 558
FaqI GGGAC 1 cut(s) 180
FatI CATG 1 cut(s) 54
FauI CCCGC 2 cut(s) 376, 461
Fnu4HI GCNGC 2 cut(s) 238, 446
FokI GGATG 3 cut(s) 92, 140, 557
FriOI GRGCYC 1 cut(s) 404
Fsp4HI GCNGC 2 cut(s) 238, 446
FspBI CTAG 3 cut(s) 50, 420, 492
GlaI GCGC 1 cut(s) 178
GluI GCNGC 2 cut(s) 238, 446
GsaI CCCAGC 2 cut(s) 177, 201
HaeIII GGCC 3 cut(s) 38, 59, 273
HapII CCGG 1 cut(s) 275
HgaI GACGC 3 cut(s) 136, 344, 451
HhaI GCGC 1 cut(s) 179
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 1 cut(s) 58
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HindIII AAGCTT 1 cut(s) 506
HinfI GANTC 4 cut(s) 278, 424, 514, 565
HpaII CCGG 1 cut(s) 275
HphI GGTGA 5 cut(s) 136, 196, 304, 367, 551
Hpy188I TCNGA 4 cut(s) 29, 345, 374, 429
Hpy188III TCNNGA 1 cut(s) 298
Hpy99I CGWCG 1 cut(s) 445
HpyAV CCTTC 3 cut(s) 115, 241, 263
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 193
HpyCH4V TGCA 1 cut(s) 78
HpyF3I CTNAG 3 cut(s) 158, 504, 509
HpySE526I ACGT 1 cut(s) 193
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 1 cut(s) 58
HspAI GCGC 1 cut(s) 177
Kzo9I GATC 1 cut(s) 294
LmnI GCTCC 1 cut(s) 399
LpnPI CCDG 8 cut(s) 20, 81, 187, 211, 220, 288, 292, 319
Lsp1109I GCAGC 1 cut(s) 249
LweI GCATC 1 cut(s) 532
MaeI CTAG 3 cut(s) 50, 420, 492
MaeII ACGT 1 cut(s) 193
MaeIII GTNAC 2 cut(s) 124, 562
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 1 cut(s) 38
MhlI GDGCHC 1 cut(s) 404
MlsI TGGCCA 1 cut(s) 59
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 3 cut(s) 287, 508, 559
MmeI TCCRAC 1 cut(s) 306
MnlI CCTC 8 cut(s) 143, 167, 212, 251, 270, 445, 467, 488
Mox20I TGGCCA 1 cut(s) 59
MscI TGGCCA 1 cut(s) 59
MseI TTAA 2 cut(s) 393, 524
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 162
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 2 cut(s) 275, 307
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 1 cut(s) 307
MvnI CGCG 3 cut(s) 179, 267, 448
NciI CCSGG 1 cut(s) 275
NcoI CCATGG 1 cut(s) 54
NdeII GATC 1 cut(s) 294
NlaIII CATG 1 cut(s) 58
NlaIV GGNNCC 1 cut(s) 166
NmuCI GTSAC 2 cut(s) 124, 562
PctI GAATGC 1 cut(s) 307
PfeI GAWTC 1 cut(s) 424
PkrI GCNGC 2 cut(s) 239, 447
PleI GAGTC 3 cut(s) 286, 508, 559
PpsI GAGTC 3 cut(s) 286, 508, 559
PshAI GACNNNNGTC 1 cut(s) 339
Psp124BI GAGCTC 1 cut(s) 404
Psp6I CCWGG 1 cut(s) 305
PspFI CCCAGC 2 cut(s) 173, 197
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 1 cut(s) 272
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
SacI GAGCTC 1 cut(s) 404
SaqAI TTAA 2 cut(s) 393, 524
SatI GCNGC 2 cut(s) 238, 446
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 1 cut(s) 272
SchI GAGTC 3 cut(s) 287, 508, 559
ScrFI CCNGG 2 cut(s) 275, 307
SduI GDGCHC 1 cut(s) 404
SetI ASST 7 cut(s) 126, 135, 196, 404, 456, 488, 510
SfaNI GCATC 1 cut(s) 532
SsiI CCGC 8 cut(s) 112, 162, 179, 265, 383, 446, 458, 468
SspMI CTAG 3 cut(s) 50, 420, 492
SstI GAGCTC 1 cut(s) 404
StyD4I CCNGG 2 cut(s) 273, 305
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 1 cut(s) 196
TaqI TCGA 2 cut(s) 150, 552
TatI WGTACW 1 cut(s) 360
TauI GCSGC 1 cut(s) 448
TfiI GAWTC 1 cut(s) 424
Tru1I TTAA 2 cut(s) 393, 524
Tru9I TTAA 2 cut(s) 393, 524
TscAI CASTG 2 cut(s) 19, 250
TseFI GTSAC 2 cut(s) 124, 562
TseI GCWGC 1 cut(s) 237
Tsp45I GTSAC 2 cut(s) 124, 562
TspRI CASTG 2 cut(s) 19, 250
XspI CTAG 3 cut(s) 50, 420, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.