FvH4_2g01700

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006333
Physical Location & Seq
Reverse (-)
1509599 .. 1510331
733 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01700.t1

Sequence Viewer

Length: 576 bp
ATGGAGCATAGTCGTTTCATTATTGGGTTGGCCATTACTCTCCTCCTCACAATCGCCACCACACCTTGTCACTGCAAGTACCACTCAAAAACGGTGCCCCTACACCAACTCAAAGAGAAAGTCACGCACCTCCACTTCTACCTCTTCGACATCCTCAGCGGCACCAAACCAAGCGCTATGGAGGTCGCACGTCCAAATATCACCACTGACAAGTCGGCCACGCCCTTCGGCAGCCTCTACGCCATCGACGACCCTCTCCGAGAGGGCCCTGAGACCAACTCGTCGATTGTTGGAAATGCCAAGGGGCTCTATTTGTCGGCCAGCCAAGATCCAGCTGACTTTACCATCGTTATGTACGTGGATTTTGGTTTTACTACCGGTAAGTTTAAAGGGAGCTCCTTTAGTGTGTTCTCCAGGAATCCAGTGGCAGAGTCGCCGGAGCGGGAGGTGGCTGTGGTTGGTGGGAGAGGGAAGTTTAGGCTGGCGAGAGGGTTTGCAAAGGTTAAGACTCATGACTTCAATGCTACAAATGGTGATGCCATTTTGGAGTACAAGGCAACTCTCATTCACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

192

Amino Acids

21.0

Weight (kDa)

8.82

Isoelectric Point (pI)

28.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 41 - 188 8.7e-50 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 280
AccB1I GGYRCC 2 cut(s) 94, 161
AccBSI CCGCTC 1 cut(s) 442
AciI CCGC 2 cut(s) 159, 442
AclWI GGATC 1 cut(s) 323
AcoI YGGCCR 3 cut(s) 30, 216, 318
AfaI GTAC 3 cut(s) 80, 356, 551
AfeI AGCGCT 1 cut(s) 175
AgeI ACCGGT 1 cut(s) 377
AgsI TTSAA 1 cut(s) 520
AjiI CACGTC 1 cut(s) 191
AjnI CCWGG 1 cut(s) 413
AluBI AGCT 2 cut(s) 335, 396
AluI AGCT 2 cut(s) 335, 396
Alw21I GWGCWC 1 cut(s) 398
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 1 cut(s) 323
Aor51HI AGCGCT 1 cut(s) 175
AoxI GGCC 4 cut(s) 30, 216, 265, 318
ApaI GGGCCC 1 cut(s) 269
ApeKI GCWGC 1 cut(s) 231
ArsI GACNNNNNNTTYG 2 cut(s) 105, 137
AsiGI ACCGGT 1 cut(s) 377
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 265, 266
AsuHPI GGTGA 2 cut(s) 193, 545
BaeGI GKGCMC 2 cut(s) 99, 269
BalI TGGCCA 1 cut(s) 32
BanI GGYRCC 2 cut(s) 94, 161
BanII GRGCYC 3 cut(s) 269, 309, 398
Bbv12I GWGCWC 1 cut(s) 398
BbvCI CCTCAGC 1 cut(s) 155
BbvI GCAGC 1 cut(s) 243
BccI CCATC 2 cut(s) 251, 353
BciT130I CCWGG 1 cut(s) 415
BcoDI GTCTC 1 cut(s) 266
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 2 cut(s) 160, 232
BlsI GCNGC 2 cut(s) 161, 233
Bme1390I CCNGG 1 cut(s) 415
BmgBI CACGTC 1 cut(s) 191
BmgT120I GGNCC 2 cut(s) 265, 266
BmiI GGNNCC 3 cut(s) 96, 163, 267
BmrFI CCNGG 1 cut(s) 415
BmsI GCATC 1 cut(s) 526
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpmI CTGGAG 1 cut(s) 397
Bpu10I CCTNAGC 1 cut(s) 155
BsaAI YACGTR 1 cut(s) 358
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 300
BsaWI WCCGGW 1 cut(s) 377
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 422
BseBI CCWGG 1 cut(s) 415
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 1 cut(s) 150
BseMII CTCAG 2 cut(s) 169, 261
BseNI ACTGG 1 cut(s) 422
BseRI GAGGAG 2 cut(s) 32, 35
BseSI GKGCMC 2 cut(s) 99, 269
BseXI GCAGC 1 cut(s) 243
BshFI GGCC 4 cut(s) 32, 218, 267, 320
BshNI GGYRCC 2 cut(s) 94, 161
BshTI ACCGGT 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 398
BsiSI CCGG 2 cut(s) 378, 437
BsmAI GTCTC 1 cut(s) 266
BsnI GGCC 4 cut(s) 32, 218, 267, 320
Bso31I GGTCTC 1 cut(s) 266
Bsp120I GGGCCC 1 cut(s) 265
Bsp1286I GDGCHC 4 cut(s) 99, 269, 309, 398
Bsp143I GATC 1 cut(s) 328
BspACI CCGC 2 cut(s) 159, 442
BspANI GGCC 4 cut(s) 32, 218, 267, 320
BspCNI CTCAG 2 cut(s) 168, 262
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 3 cut(s) 96, 163, 267
BspPI GGATC 1 cut(s) 323
BspT107I GGYRCC 2 cut(s) 94, 161
BspTNI GGTCTC 1 cut(s) 266
BsrBI CCGCTC 1 cut(s) 442
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 1 cut(s) 422
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 1 cut(s) 328
BssT1I CCWWGG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 415
Bst4CI ACNGT 1 cut(s) 94
Bst6I CTCTTC 1 cut(s) 149
BstBAI YACGTR 1 cut(s) 358
BstC8I GCNNGC 2 cut(s) 322, 483
BstDEI CTNAG 2 cut(s) 155, 270
BstF5I GGATG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 1 cut(s) 328
BstNI CCWGG 1 cut(s) 415
BstSCI CCNGG 1 cut(s) 413
BstSLI GKGCMC 2 cut(s) 99, 269
BstV1I GCAGC 1 cut(s) 243
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuRI GGCC 4 cut(s) 32, 218, 267, 320
BtrI CACGTC 1 cut(s) 191
BtsCI GGATG 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 3 cut(s) 70, 204, 429
Cac8I GCNNGC 2 cut(s) 322, 483
CciI TCATGA 1 cut(s) 511
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 2 cut(s) 265, 266
Csp6I GTAC 3 cut(s) 79, 355, 550
CspAI ACCGGT 1 cut(s) 377
CviAII CATG 1 cut(s) 512
CviQI GTAC 3 cut(s) 79, 355, 550
DdeI CTNAG 2 cut(s) 155, 270
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
DraI TTTAAA 1 cut(s) 388
DrdI GACNNNNNNGTC 1 cut(s) 280
DseDI GACNNNNNNGTC 1 cut(s) 280
EaeI YGGCCR 3 cut(s) 30, 216, 318
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Ecl136II GAGCTC 1 cut(s) 396
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 3 cut(s) 269, 309, 398
Eco31I GGTCTC 1 cut(s) 266
Eco47III AGCGCT 1 cut(s) 175
Eco53kI GAGCTC 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 396
EcoO109I RGGNCCY 2 cut(s) 265, 266
EcoRII CCWGG 1 cut(s) 413
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 3 cut(s) 269, 309, 398
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 1 cut(s) 515
FaiI YATR 4 cut(s) 9, 179, 353, 513
FatI CATG 1 cut(s) 511
FauI CCCGC 1 cut(s) 435
Fnu4HI GCNGC 2 cut(s) 160, 232
FokI GGATG 1 cut(s) 137
FriOI GRGCYC 3 cut(s) 269, 309, 398
Fsp4HI GCNGC 2 cut(s) 160, 232
GlaI GCGC 1 cut(s) 175
GluI GCNGC 2 cut(s) 160, 232
GsuI CTGGAG 1 cut(s) 397
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 4 cut(s) 32, 218, 267, 320
HapII CCGG 2 cut(s) 378, 437
HhaI GCGC 1 cut(s) 176
Hin1II CATG 1 cut(s) 515
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HinfI GANTC 3 cut(s) 418, 431, 508
HpaII CCGG 2 cut(s) 378, 437
HphI GGTGA 2 cut(s) 193, 545
Hpy188I TCNGA 1 cut(s) 260
Hpy188III TCNNGA 1 cut(s) 512
Hpy99I CGWCG 2 cut(s) 251, 286
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4IV ACGT 2 cut(s) 190, 357
HpyCH4V TGCA 2 cut(s) 75, 497
HpyF3I CTNAG 2 cut(s) 155, 270
HpySE526I ACGT 2 cut(s) 190, 357
Hsp92II CATG 1 cut(s) 515
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 1 cut(s) 328
LmnI GCTCC 4 cut(s) 4, 393, 401, 439
LpnPI CCDG 9 cut(s) 282, 334, 345, 391, 400, 427, 435, 450, 467
Lsp1109I GCAGC 1 cut(s) 243
LweI GCATC 1 cut(s) 526
MaeII ACGT 2 cut(s) 190, 357
MaeIII GTNAC 2 cut(s) 68, 121
MalI GATC 1 cut(s) 330
MbiI CCGCTC 1 cut(s) 442
MboI GATC 1 cut(s) 328
MboII GAAGA 1 cut(s) 136
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 4 cut(s) 99, 269, 309, 398
MlsI TGGCCA 1 cut(s) 32
MluNI TGGCCA 1 cut(s) 32
MlyI GAGTC 2 cut(s) 440, 502
MmeI TCCRAC 1 cut(s) 271
Mox20I TGGCCA 1 cut(s) 32
MscI TGGCCA 1 cut(s) 32
MseI TTAA 2 cut(s) 387, 504
MslI CAYNNNNRTG 1 cut(s) 350
Msp20I TGGCCA 1 cut(s) 32
MspA1I CMGCKG 2 cut(s) 159, 335
MspI CCGG 2 cut(s) 378, 437
MspR9I CCNGG 1 cut(s) 415
MvaI CCWGG 1 cut(s) 415
NdeII GATC 1 cut(s) 328
NlaIII CATG 1 cut(s) 515
NlaIV GGNNCC 3 cut(s) 96, 163, 267
NmuCI GTSAC 2 cut(s) 68, 121
PagI TCATGA 1 cut(s) 511
PcsI WCGNNNNNNNCGW 2 cut(s) 246, 354
PfeI GAWTC 1 cut(s) 418
PfoI TCCNGGA 1 cut(s) 413
PinAI ACCGGT 1 cut(s) 377
PkrI GCNGC 2 cut(s) 161, 233
PleI GAGTC 2 cut(s) 439, 502
PpsI GAGTC 2 cut(s) 439, 502
Ppu21I YACGTR 1 cut(s) 358
Psp124BI GAGCTC 1 cut(s) 398
Psp6I CCWGG 1 cut(s) 413
PspGI CCWGG 1 cut(s) 413
PspN4I GGNNCC 3 cut(s) 96, 163, 267
PspOMI GGGCCC 1 cut(s) 265
PspPI GGNCC 2 cut(s) 265, 266
PsuI RGATCY 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 3 cut(s) 80, 356, 551
RsaNI GTAC 3 cut(s) 79, 355, 550
RseI CAYNNNNRTG 1 cut(s) 350
SacI GAGCTC 1 cut(s) 398
SaqAI TTAA 2 cut(s) 387, 504
SatI GCNGC 2 cut(s) 160, 232
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 2 cut(s) 265, 266
SchI GAGTC 2 cut(s) 440, 502
ScrFI CCNGG 1 cut(s) 415
SduI GDGCHC 4 cut(s) 99, 269, 309, 398
SfaNI GCATC 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 350
SsiI CCGC 2 cut(s) 159, 442
SstI GAGCTC 1 cut(s) 398
StyD4I CCNGG 1 cut(s) 413
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 94
TaiI ACGT 2 cut(s) 193, 360
TaqI TCGA 3 cut(s) 147, 246, 284
TatI WGTACW 1 cut(s) 549
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 1 cut(s) 418
Tru1I TTAA 2 cut(s) 387, 504
Tru9I TTAA 2 cut(s) 387, 504
TscAI CASTG 3 cut(s) 77, 211, 429
TseFI GTSAC 2 cut(s) 68, 121
TseI GCWGC 1 cut(s) 231
Tsp45I GTSAC 2 cut(s) 68, 121
TspDTI ATGAA 1 cut(s) 7
TspRI CASTG 3 cut(s) 77, 211, 429
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.