Rroxscaffold_7G00215660

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006333
Physical Location & Seq
Forward (+)
66230849 .. 66231424
576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215660.1

Sequence Viewer

Length: 576 bp
ATGGAGCGTAGTCTTTTGATTTTTGGGTTAGCCTTTACTATCCTTCTAACCATCGTCACCACACCTTCGCACTGCAAATACCACTCAAAAACAGTCCCTGTAGACCGCCTCAAAGAGAAAGTCACGCAACTCCACTTCTACCTCTTCGACATCCTCAGCGGCACCAAACCCAGCGCCGTGGAGATCGCACGTCCGAACGTCACCACTGACAAGTCAGCCACCCCTTTCGGGAGCCTCTATGCCTTCGATGACCATCTCCGAGAGGGCCCCGAGACCAACTCATCGATAGTCGGGAATGCGAAGGGGCTCTACTTGTCCGCCAGCCAAGACCCAGCTAACTTTACCATCGTGATGTACGCAGATTTCGGTTTTTCTACCGGTAAGTTTAAAGGAAGCTCATTTGGCGTGTTTTCAAGGAATCCAGTGGCTGAGTCGCCGGAACGCGAGGTGGCTGTGGTTGGTGGGAGAGGGATGTTTAGGCTAGCTAGAGGGTTTGCTAGGGTTAAGACTCATTACTTCAATGCTACTAATGGTGATGCCATTTTGGAGTATAAGGCAACCATCATTCACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

191

Amino Acids

21.05

Weight (kDa)

9.16

Isoelectric Point (pI)

33.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 41 - 188 6.4e-47 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccI GTMKAC 1 cut(s) 102
AccII CGCG 1 cut(s) 444
AciI CCGC 3 cut(s) 106, 159, 318
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 228
AgeI ACCGGT 1 cut(s) 377
AgsI TTSAA 2 cut(s) 414, 520
AjiI CACGTC 1 cut(s) 191
AluBI AGCT 3 cut(s) 335, 396, 485
AluI AGCT 3 cut(s) 335, 396, 485
Alw26I GTCTC 1 cut(s) 266
AlwNI CAGNNNCTG 2 cut(s) 98, 428
Ama87I CYCGRG 1 cut(s) 269
AoxI GGCC 1 cut(s) 265
ApaI GGGCCC 1 cut(s) 269
ArsI GACNNNNNNTTYG 2 cut(s) 105, 137
AsiGI ACCGGT 1 cut(s) 377
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 265, 266
AsuHPI GGTGA 3 cut(s) 49, 193, 545
AsuNHI GCTAGC 1 cut(s) 481
AvaI CYCGRG 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 269
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 2 cut(s) 269, 309
BarI GAAGNNNNNNTAC 2 cut(s) 293, 325
BbvCI CCTCAGC 1 cut(s) 155
BccI CCATC 4 cut(s) 59, 261, 353, 569
BceAI ACGGC 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 3 cut(s) 482, 486, 498
BfmI CTRYAG 1 cut(s) 99
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 1 cut(s) 160
BlsI GCNGC 1 cut(s) 161
BmeT110I CYCGRG 1 cut(s) 269
BmgBI CACGTC 1 cut(s) 191
BmgT120I GGNCC 2 cut(s) 265, 266
BmiI GGNNCC 4 cut(s) 163, 233, 267, 268
BmsI GCATC 1 cut(s) 526
BmtI GCTAGC 1 cut(s) 485
BplI GAGNNNNNCTC 2 cut(s) 263, 295
Bpu10I CCTNAGC 1 cut(s) 155
Bsa29I ATCGAT 1 cut(s) 284
BsaBI GATNNNNATC 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 177
BsaWI WCCGGW 1 cut(s) 377
Bsc4I CCNNNNNNNGG 1 cut(s) 228
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 422
Bse8I GATNNNNATC 1 cut(s) 252
BseCI ATCGAT 1 cut(s) 284
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 2 cut(s) 150, 477
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 169, 420
BseNI ACTGG 1 cut(s) 422
BseSI GKGCMC 1 cut(s) 269
BseYI CCCAGC 2 cut(s) 170, 331
Bsh1236I CGCG 1 cut(s) 444
BshFI GGCC 1 cut(s) 267
BshNI GGYRCC 1 cut(s) 161
BshTI ACCGGT 1 cut(s) 377
BshVI ATCGAT 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 2 cut(s) 378, 437
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 228
BsmAI GTCTC 1 cut(s) 266
BsmFI GGGAC 1 cut(s) 80
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 1 cut(s) 267
Bso31I GGTCTC 1 cut(s) 266
BsoBI CYCGRG 1 cut(s) 269
Bsp120I GGGCCC 1 cut(s) 265
Bsp1286I GDGCHC 2 cut(s) 269, 309
Bsp143I GATC 1 cut(s) 183
BspACI CCGC 3 cut(s) 106, 159, 318
BspANI GGCC 1 cut(s) 267
BspCNI CTCAG 2 cut(s) 168, 421
BspDI ATCGAT 1 cut(s) 284
BspFNI CGCG 1 cut(s) 444
BspLI GGNNCC 4 cut(s) 163, 233, 267, 268
BspOI GCTAGC 1 cut(s) 485
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 266
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 1 cut(s) 422
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 1 cut(s) 177
BssMI GATC 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 94
Bst6I CTCTTC 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 322, 483
BstDEI CTNAG 2 cut(s) 155, 429
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 2 cut(s) 150, 477
BstFNI CGCG 1 cut(s) 444
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 1 cut(s) 186
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 1 cut(s) 183
BstMWI GCNNNNNNNGC 1 cut(s) 402
BstSFI CTRYAG 1 cut(s) 99
BstSLI GKGCMC 1 cut(s) 269
BstUI CGCG 1 cut(s) 444
BstXI CCANNNNNNTGG 1 cut(s) 178
Bsu15I ATCGAT 1 cut(s) 284
BsuRI GGCC 1 cut(s) 267
BsuTUI ATCGAT 1 cut(s) 284
BtgI CCRYGG 1 cut(s) 177
BtrI CACGTC 1 cut(s) 191
BtsCI GGATG 2 cut(s) 150, 477
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 3 cut(s) 70, 204, 429
Cac8I GCNNGC 2 cut(s) 322, 483
CaiI CAGNNNCTG 2 cut(s) 98, 428
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 2 cut(s) 265, 266
ClaI ATCGAT 1 cut(s) 284
Csp6I GTAC 1 cut(s) 355
CspAI ACCGGT 1 cut(s) 377
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 2 cut(s) 155, 429
DpnI GATC 1 cut(s) 185
DpnII GATC 1 cut(s) 183
DraI TTTAAA 1 cut(s) 388
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
EciI GGCGGA 1 cut(s) 307
Eco24I GRGCYC 2 cut(s) 269, 309
Eco31I GGTCTC 1 cut(s) 266
Eco88I CYCGRG 1 cut(s) 269
EcoO109I RGGNCCY 2 cut(s) 265, 266
EcoT38I GRGCYC 2 cut(s) 269, 309
FaiI YATR 2 cut(s) 240, 552
FaqI GGGAC 1 cut(s) 80
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 160
FokI GGATG 2 cut(s) 137, 484
FriOI GRGCYC 2 cut(s) 269, 309
Fsp4HI GCNGC 1 cut(s) 160
FspBI CTAG 3 cut(s) 482, 486, 498
GlaI GCGC 1 cut(s) 175
GluI GCNGC 1 cut(s) 160
GsaI CCCAGC 2 cut(s) 174, 335
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 1 cut(s) 267
HapII CCGG 2 cut(s) 378, 437
HhaI GCGC 1 cut(s) 176
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HinfI GANTC 3 cut(s) 418, 431, 508
HpaII CCGG 2 cut(s) 378, 437
HphI GGTGA 3 cut(s) 49, 193, 545
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 2 cut(s) 195, 260
Hpy188III TCNNGA 3 cut(s) 229, 292, 349
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 4 cut(s) 53, 75, 253, 295
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4IV ACGT 2 cut(s) 190, 198
HpyCH4V TGCA 1 cut(s) 75
HpyF10VI GCNNNNNNNGC 1 cut(s) 402
HpyF3I CTNAG 2 cut(s) 155, 429
HpySE526I ACGT 2 cut(s) 190, 198
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 1 cut(s) 183
LmnI GCTCC 2 cut(s) 4, 231
LpnPI CCDG 7 cut(s) 111, 184, 334, 345, 391, 435, 450
LweI GCATC 1 cut(s) 526
MaeI CTAG 3 cut(s) 482, 486, 498
MaeII ACGT 2 cut(s) 190, 198
MaeIII GTNAC 3 cut(s) 55, 121, 199
MalI GATC 1 cut(s) 185
MboI GATC 1 cut(s) 183
MboII GAAGA 1 cut(s) 136
MhlI GDGCHC 2 cut(s) 269, 309
MlyI GAGTC 2 cut(s) 440, 502
MnlI CCTC 8 cut(s) 119, 152, 164, 245, 256, 439, 461, 482
MseI TTAA 2 cut(s) 387, 504
MslI CAYNNNNRTG 1 cut(s) 350
MspA1I CMGCKG 1 cut(s) 159
MspI CCGG 2 cut(s) 378, 437
Mva1269I GAATGC 1 cut(s) 301
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 1 cut(s) 402
NdeII GATC 1 cut(s) 183
NheI GCTAGC 1 cut(s) 481
NlaIV GGNNCC 4 cut(s) 163, 233, 267, 268
NmuCI GTSAC 3 cut(s) 55, 121, 199
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 418
PinAI ACCGGT 1 cut(s) 377
PkrI GCNGC 1 cut(s) 161
PleI GAGTC 2 cut(s) 439, 502
PpsI GAGTC 2 cut(s) 439, 502
PspFI CCCAGC 2 cut(s) 170, 331
PspN4I GGNNCC 4 cut(s) 163, 233, 267, 268
PspOMI GGGCCC 1 cut(s) 265
PspPI GGNCC 2 cut(s) 265, 266
PstNI CAGNNNCTG 2 cut(s) 98, 428
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
RseI CAYNNNNRTG 1 cut(s) 350
SaqAI TTAA 2 cut(s) 387, 504
SatI GCNGC 1 cut(s) 160
Sau3AI GATC 1 cut(s) 183
Sau96I GGNCC 2 cut(s) 265, 266
SchI GAGTC 2 cut(s) 440, 502
SduI GDGCHC 2 cut(s) 269, 309
SetI ASST 8 cut(s) 67, 144, 193, 201, 337, 398, 450, 487
SfaNI GCATC 1 cut(s) 526
SfcI CTRYAG 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 350
SsiI CCGC 3 cut(s) 106, 159, 318
SspMI CTAG 3 cut(s) 482, 486, 498
TaaI ACNGT 1 cut(s) 94
TaiI ACGT 2 cut(s) 193, 201
TaqI TCGA 3 cut(s) 147, 246, 284
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 1 cut(s) 418
Tru1I TTAA 2 cut(s) 387, 504
Tru9I TTAA 2 cut(s) 387, 504
TscAI CASTG 3 cut(s) 77, 211, 429
TseFI GTSAC 3 cut(s) 55, 121, 199
Tsp45I GTSAC 3 cut(s) 55, 121, 199
TspRI CASTG 3 cut(s) 77, 211, 429
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 3 cut(s) 482, 486, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.