Rorug05G0509700

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006333
Physical Location & Seq
Reverse (-)
69295063 .. 69300373
5311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0509700.1

Sequence Viewer

Length: 720 bp
ATGACTGCTACCATTTTTGCAGAACTCAGAGATGGGAACGGAGCCAGCAACGCTGTTATTGCTGACATTGAAAGTGACGAAAGAGCCTACAGCGACGGATGGGGCAACTTAGATCATAGTGAAAAGTTGGAAACATATAGTCATGGCAGATATGGTATTATTGCTCCGACTCTTGTAGAAGACGAAGGTACTAGTGTGGTTGAATTGGAATTGACCAAGTCCATTGCAGCAAAGCTTCGCCCCGAATCACCATTGCCTGATTCGTGTTGCATATTCAAAGTTCCCGAGGTGAAGCAAAGGCGAAACAGAGAAGCATATCGACTTGATGTTGTCTCAATCGGACCCTTACACAGAGGCTGCAATGAACAATATCAATTCATGGAAAATGTGAAACTATGGTATTTGCATTGTATCCTTTCAAGAACGAGTGGTATAAGTTTGGGAAGCTTGGTCACAGCCATTATGAAGCTTGAGAAAGAAGCCCGTGATTGTTATGCAGAATCATTGGATCATCTCAATAAAAAAGTCTTTGTTGAGATGCTGATTATTGATGGTTGCTTCCTAATAAAACTATTTGACAAGCTACCTCACTATTATGTACAAGAAAAGATGAACGACCCCATTTTCACCATATCAGGTATGCTCGACTGCGTATACCATGATATTGTGTTGCTACAAAACCAACTATCTTGGTTTGTGCTCGAGGTTTTGTACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

239

Amino Acids

27.23

Weight (kDa)

5.11

Isoelectric Point (pI)

30.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 91 - 238 5.4e-40 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 654
AclWI GGATC 1 cut(s) 516
AfaI GTAC 3 cut(s) 190, 600, 713
AgsI TTSAA 4 cut(s) 71, 203, 277, 420
AhlI ACTAGT 1 cut(s) 191
AluBI AGCT 4 cut(s) 235, 447, 469, 583
AluI AGCT 4 cut(s) 235, 447, 469, 583
Alw21I GWGCWC 1 cut(s) 702
Alw26I GTCTC 1 cut(s) 337
AlwI GGATC 1 cut(s) 516
AlwNI CAGNNNCTG 1 cut(s) 357
Ama87I CYCGRG 2 cut(s) 284, 701
ApeKI GCWGC 2 cut(s) 227, 357
AspS9I GGNCC 1 cut(s) 341
AsuHPI GGTGA 3 cut(s) 240, 301, 619
AvaI CYCGRG 2 cut(s) 284, 701
AvaII GGWCC 1 cut(s) 341
BaeI ACNNNNGTAYC 2 cut(s) 180, 213
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 702
BbvI GCAGC 2 cut(s) 239, 344
BccI CCATC 3 cut(s) 26, 93, 545
BciVI GTATCC 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 337
BcuI ACTAGT 1 cut(s) 191
BfaI CTAG 1 cut(s) 192
BfmI CTRYAG 1 cut(s) 88
BfuI GTATCC 1 cut(s) 422
BisI GCNGC 2 cut(s) 228, 358
BlsI GCNGC 2 cut(s) 229, 359
Bme18I GGWCC 1 cut(s) 341
BmeT110I CYCGRG 2 cut(s) 284, 701
BmgT120I GGNCC 1 cut(s) 341
BmiI GGNNCC 2 cut(s) 43, 343
BmsI GCATC 1 cut(s) 528
BpiI GAAGAC 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 491
BsaJI CCNNGG 1 cut(s) 285
Bse3DI GCAATG 3 cut(s) 222, 251, 367
BseDI CCNNGG 1 cut(s) 285
BseGI GGATG 1 cut(s) 104
BseMI GCAATG 3 cut(s) 222, 251, 367
BseMII CTCAG 1 cut(s) 40
BseXI GCAGC 2 cut(s) 239, 344
BsiHKAI GWGCWC 1 cut(s) 702
BsiHKCI CYCGRG 2 cut(s) 284, 701
BsmAI GTCTC 1 cut(s) 337
BsoBI CYCGRG 2 cut(s) 284, 701
Bsp1286I GDGCHC 1 cut(s) 702
Bsp1407I TGTACA 2 cut(s) 598, 711
Bsp143I GATC 2 cut(s) 112, 508
BspCNI CTCAG 1 cut(s) 39
BspLI GGNNCC 2 cut(s) 43, 343
BspPI GGATC 1 cut(s) 516
BsrDI GCAATG 3 cut(s) 222, 251, 367
BsrGI TGTACA 2 cut(s) 598, 711
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 2 cut(s) 112, 508
BssNAI GTATAC 1 cut(s) 655
Bst1107I GTATAC 1 cut(s) 655
BstAUI TGTACA 2 cut(s) 598, 711
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 2 cut(s) 26, 109
BstF5I GGATG 1 cut(s) 104
BstKTI GATC 2 cut(s) 115, 511
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 2 cut(s) 112, 508
BstMWI GCNNNNNNNGC 2 cut(s) 50, 59
BstSFI CTRYAG 1 cut(s) 88
BstV1I GCAGC 2 cut(s) 239, 344
BstV2I GAAGAC 1 cut(s) 186
BstZ17I GTATAC 1 cut(s) 655
BsuI GTATCC 1 cut(s) 422
BtsCI GGATG 1 cut(s) 104
Cac8I GCNNGC 1 cut(s) 46
CaiI CAGNNNCTG 1 cut(s) 357
Cfr13I GGNCC 1 cut(s) 341
Csp6I GTAC 3 cut(s) 189, 599, 712
CviAII CATG 3 cut(s) 143, 379, 659
CviJI RGCY 9 cut(s) 44, 86, 235, 357, 447, 458, 469, 482, 583
CviKI_1 RGCY 9 cut(s) 44, 86, 235, 357, 447, 458, 469, 482, 583
CviQI GTAC 3 cut(s) 189, 599, 712
DdeI CTNAG 2 cut(s) 26, 109
DpnI GATC 2 cut(s) 114, 510
DpnII GATC 2 cut(s) 112, 508
Eco47I GGWCC 1 cut(s) 341
Eco88I CYCGRG 2 cut(s) 284, 701
FaeI CATG 3 cut(s) 146, 382, 662
FatI CATG 3 cut(s) 142, 378, 658
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 2 cut(s) 228, 358
FokI GGATG 1 cut(s) 111
Fsp4HI GCNGC 2 cut(s) 228, 358
FspBI CTAG 1 cut(s) 192
GluI GCNGC 2 cut(s) 228, 358
Hin1II CATG 3 cut(s) 146, 382, 662
HindIII AAGCTT 3 cut(s) 233, 445, 467
HinfI GANTC 4 cut(s) 169, 245, 260, 500
HphI GGTGA 3 cut(s) 240, 301, 619
Hpy166II GTNNAC 1 cut(s) 655
Hpy188I TCNGA 3 cut(s) 29, 168, 341
Hpy188III TCNNGA 2 cut(s) 284, 420
Hpy8I GTNNAC 1 cut(s) 655
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 1 cut(s) 179
HpyCH4V TGCA 6 cut(s) 20, 227, 270, 360, 406, 497
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 59
HpyF3I CTNAG 2 cut(s) 26, 109
Hsp92II CATG 3 cut(s) 146, 382, 662
Kzo9I GATC 2 cut(s) 112, 508
LmnI GCTCC 2 cut(s) 41, 169
LpnPI CCDG 3 cut(s) 58, 270, 621
Lsp1109I GCAGC 2 cut(s) 239, 344
LweI GCATC 1 cut(s) 528
MaeI CTAG 1 cut(s) 192
MaeIII GTNAC 2 cut(s) 74, 451
MalI GATC 2 cut(s) 114, 510
MboI GATC 2 cut(s) 112, 508
MboII GAAGA 1 cut(s) 191
MhlI GDGCHC 1 cut(s) 702
MluCI AATT 3 cut(s) 203, 209, 374
MlyI GAGTC 1 cut(s) 163
MmeI TCCRAC 2 cut(s) 108, 191
MnlI CCTC 4 cut(s) 280, 347, 597, 697
MslI CAYNNNNRTG 1 cut(s) 594
MwoI GCNNNNNNNGC 2 cut(s) 50, 59
NdeII GATC 2 cut(s) 112, 508
NlaIII CATG 3 cut(s) 146, 382, 662
NlaIV GGNNCC 2 cut(s) 43, 343
NmuCI GTSAC 2 cut(s) 74, 451
PaeR7I CTCGAG 1 cut(s) 701
PfeI GAWTC 3 cut(s) 245, 260, 500
PflFI GACNNNGTC 1 cut(s) 217
PkrI GCNGC 2 cut(s) 229, 359
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
PspN4I GGNNCC 2 cut(s) 43, 343
PspPI GGNCC 1 cut(s) 341
PspXI VCTCGAGB 1 cut(s) 701
PstNI CAGNNNCTG 1 cut(s) 357
PsyI GACNNNGTC 1 cut(s) 217
RsaI GTAC 3 cut(s) 190, 600, 713
RsaNI GTAC 3 cut(s) 189, 599, 712
RseI CAYNNNNRTG 1 cut(s) 594
SatI GCNGC 2 cut(s) 228, 358
Sau3AI GATC 2 cut(s) 112, 508
Sau96I GGNCC 1 cut(s) 341
SchI GAGTC 1 cut(s) 163
SduI GDGCHC 1 cut(s) 702
SetI ASST 9 cut(s) 190, 237, 291, 449, 471, 585, 589, 640, 708
SfaNI GCATC 1 cut(s) 528
SfcI CTRYAG 1 cut(s) 88
Sfr274I CTCGAG 1 cut(s) 701
SinI GGWCC 1 cut(s) 341
SlaI CTCGAG 1 cut(s) 701
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 2 cut(s) 470, 701
SmoI CTYRAG 2 cut(s) 470, 701
SpeI ACTAGT 1 cut(s) 191
Sse9I AATT 3 cut(s) 203, 209, 374
SspMI CTAG 1 cut(s) 192
TaqI TCGA 3 cut(s) 319, 645, 702
TasI AATT 3 cut(s) 203, 209, 374
TatI WGTACW 2 cut(s) 598, 711
TfiI GAWTC 3 cut(s) 245, 260, 500
TseFI GTSAC 2 cut(s) 74, 451
TseI GCWGC 2 cut(s) 227, 357
Tsp45I GTSAC 2 cut(s) 74, 451
TspDTI ATGAA 4 cut(s) 367, 378, 479, 626
TspGWI ACGGA 2 cut(s) 54, 111
Tth111I GACNNNGTC 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 341
XhoI CTCGAG 1 cut(s) 701
XmiI GTMKAC 1 cut(s) 654
XspI CTAG 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.