MD05G1117400.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006333
Physical Location & Seq
Forward (+)
23828353 .. 23828925
573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1117400.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGAACGCGGTAGTTTGGTTCTTGGATTGGCCTTGACTCTCCTAACCGTCGCGGTCACACCAACACATTGCAGCTACCATTCCCACACACTTCCAAATGTCCATCAAGAAGAAAAGGTCACGCGCCTCCACTTCTACCTCTTCGACATCATCAGCGGCGACAAACCTAGTGCTGTAGAAGTCGCACGTCCATACAGGACGAAGAATGACAGTTCCCCCACGCCGTTCGGCAGCGTGTGGGCCATCGACGACCCTCTACGAGAGGGCCCTGAGGCCACTTCCAAAGTTATTGGGAACGCTCAAGGGCTCTACGTGTCGTCAGTCCAGGATGAGAGCAGGCTGGGGCTTGTAATGTACGTTGATTTCGGTTTTACTGAGGGTAAGTTTAAGGGGAGCTCATTCAGCGTGTTTTCGAGAAATCCGGTGACGGAGACGAATCGGGAGTTGGCCGTTGTGGGAGGGAGAGGGAGGTTTAGAATGGCGAGAGGGTTTGCGAAGCTGAAAACTAGTTATTTAAATGTTACCAATGGTGATGCCATTATTGAGTATAAGGTCACTCTCATCCACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

191

Amino Acids

21.02

Weight (kDa)

8.65

Isoelectric Point (pI)

28.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 40 - 187 8.7e-52 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 9, 53, 124
AciI CCGC 3 cut(s) 9, 53, 156
AcoI YGGCCR 1 cut(s) 447
AfaI GTAC 1 cut(s) 356
AflIII ACRYGT 1 cut(s) 312
AhlI ACTAGT 1 cut(s) 506
AjiI CACGTC 1 cut(s) 188
AjnI CCWGG 1 cut(s) 324
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
AluBI AGCT 3 cut(s) 75, 396, 499
AluI AGCT 3 cut(s) 75, 396, 499
Alw21I GWGCWC 1 cut(s) 398
Alw26I GTCTC 1 cut(s) 425
AoxI GGCC 5 cut(s) 30, 240, 265, 273, 447
ApaI GGGCCC 1 cut(s) 269
ApeKI GCWGC 2 cut(s) 72, 231
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 3 cut(s) 240, 265, 266
AsuHPI GGTGA 2 cut(s) 436, 542
AxyI CCTNAGG 1 cut(s) 270
BaeGI GKGCMC 1 cut(s) 269
BanII GRGCYC 3 cut(s) 269, 309, 398
Bbv12I GWGCWC 1 cut(s) 398
BbvI GCAGC 2 cut(s) 84, 243
BccI CCATC 2 cut(s) 111, 251
BceAI ACGGC 2 cut(s) 208, 434
BciT130I CCWGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 425
BcuI ACTAGT 1 cut(s) 506
BfaI CTAG 2 cut(s) 168, 507
BfmI CTRYAG 1 cut(s) 174
BisI GCNGC 3 cut(s) 73, 157, 232
BlsI GCNGC 3 cut(s) 74, 158, 233
Bme1390I CCNGG 1 cut(s) 326
BmgBI CACGTC 1 cut(s) 188
BmgT120I GGNCC 3 cut(s) 240, 265, 266
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 1 cut(s) 523
BpuEI CTTGAG 1 cut(s) 285
BsaAI YACGTR 1 cut(s) 313
BsaWI WCCGGW 1 cut(s) 421
Bse21I CCTNAGG 1 cut(s) 270
Bse3DI GCAATG 1 cut(s) 67
BseBI CCWGG 1 cut(s) 326
BseGI GGATG 2 cut(s) 334, 561
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 2 cut(s) 261, 366
BseSI GKGCMC 1 cut(s) 269
BseXI GCAGC 2 cut(s) 84, 243
BseYI CCCAGC 1 cut(s) 340
Bsh1236I CGCG 3 cut(s) 9, 53, 124
BshFI GGCC 5 cut(s) 32, 242, 267, 275, 449
BsiHKAI GWGCWC 1 cut(s) 398
BsiSI CCGG 1 cut(s) 422
BsmAI GTCTC 1 cut(s) 425
BsmBI CGTCTC 1 cut(s) 425
BsnI GGCC 5 cut(s) 32, 242, 267, 275, 449
Bsp120I GGGCCC 1 cut(s) 265
Bsp1286I GDGCHC 3 cut(s) 269, 309, 398
BspACI CCGC 3 cut(s) 9, 53, 156
BspANI GGCC 5 cut(s) 32, 242, 267, 275, 449
BspCNI CTCAG 2 cut(s) 262, 367
BspFNI CGCG 3 cut(s) 9, 53, 124
BspLI GGNNCC 1 cut(s) 267
BsrDI GCAATG 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 2 cut(s) 49, 212
Bst6I CTCTTC 1 cut(s) 146
BstBAI YACGTR 1 cut(s) 313
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 2 cut(s) 270, 375
BstF5I GGATG 2 cut(s) 334, 561
BstFNI CGCG 3 cut(s) 9, 53, 124
BstHHI GCGC 1 cut(s) 126
BstMAI GTCTC 1 cut(s) 425
BstMWI GCNNNNNNNGC 1 cut(s) 402
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 324
BstSFI CTRYAG 1 cut(s) 174
BstSLI GKGCMC 1 cut(s) 269
BstUI CGCG 3 cut(s) 9, 53, 124
BstV1I GCAGC 2 cut(s) 84, 243
Bsu36I CCTNAGG 1 cut(s) 270
BsuRI GGCC 5 cut(s) 32, 242, 267, 275, 449
BtrI CACGTC 1 cut(s) 188
BtsCI GGATG 2 cut(s) 334, 561
Cac8I GCNNGC 1 cut(s) 338
CfoI GCGC 1 cut(s) 126
Cfr13I GGNCC 3 cut(s) 240, 265, 266
Csp6I GTAC 1 cut(s) 355
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 2 cut(s) 270, 375
DraI TTTAAA 1 cut(s) 516
EaeI YGGCCR 1 cut(s) 447
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
Ecl136II GAGCTC 1 cut(s) 396
Eco24I GRGCYC 3 cut(s) 269, 309, 398
Eco53kI GAGCTC 1 cut(s) 396
Eco81I CCTNAGG 1 cut(s) 270
EcoICRI GAGCTC 1 cut(s) 396
EcoO109I RGGNCCY 2 cut(s) 265, 266
EcoRII CCWGG 1 cut(s) 324
EcoT38I GRGCYC 3 cut(s) 269, 309, 398
Esp3I CGTCTC 1 cut(s) 425
FaiI YATR 2 cut(s) 193, 549
Fnu4HI GCNGC 3 cut(s) 73, 157, 232
FokI GGATG 2 cut(s) 341, 548
FriOI GRGCYC 3 cut(s) 269, 309, 398
Fsp4HI GCNGC 3 cut(s) 73, 157, 232
FspBI CTAG 2 cut(s) 168, 507
GlaI GCGC 1 cut(s) 125
GluI GCNGC 3 cut(s) 73, 157, 232
GsaI CCCAGC 1 cut(s) 344
HaeIII GGCC 5 cut(s) 32, 242, 267, 275, 449
HapII CCGG 1 cut(s) 422
HhaI GCGC 1 cut(s) 126
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HinfI GANTC 2 cut(s) 37, 436
HpaII CCGG 1 cut(s) 422
HphI GGTGA 2 cut(s) 436, 542
Hpy188III TCNNGA 3 cut(s) 107, 414, 440
Hpy99I CGWCG 2 cut(s) 53, 251
HpyCH4III ACNGT 2 cut(s) 49, 212
HpyCH4IV ACGT 3 cut(s) 187, 312, 357
HpyCH4V TGCA 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 1 cut(s) 402
HpyF3I CTNAG 2 cut(s) 270, 375
HpySE526I ACGT 3 cut(s) 187, 312, 357
HspAI GCGC 1 cut(s) 124
LmnI GCTCC 1 cut(s) 393
LpnPI CCDG 7 cut(s) 181, 282, 311, 322, 326, 338, 435
Lsp1109I GCAGC 2 cut(s) 84, 243
LweI GCATC 1 cut(s) 523
MaeI CTAG 2 cut(s) 168, 507
MaeII ACGT 3 cut(s) 187, 312, 357
MaeIII GTNAC 5 cut(s) 55, 118, 424, 520, 553
MboII GAAGA 3 cut(s) 122, 133, 214
MhlI GDGCHC 3 cut(s) 269, 309, 398
MlyI GAGTC 1 cut(s) 31
MseI TTAA 2 cut(s) 387, 515
MspA1I CMGCKG 1 cut(s) 156
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 1 cut(s) 326
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 3 cut(s) 9, 53, 124
MwoI GCNNNNNNNGC 1 cut(s) 402
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 4 cut(s) 55, 118, 424, 553
PfeI GAWTC 1 cut(s) 436
PfoI TCCNGGA 1 cut(s) 324
PkrI GCNGC 3 cut(s) 74, 158, 233
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Ppu21I YACGTR 1 cut(s) 313
Psp124BI GAGCTC 1 cut(s) 398
Psp6I CCWGG 1 cut(s) 324
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 267
PspOMI GGGCCC 1 cut(s) 265
PspPI GGNCC 3 cut(s) 240, 265, 266
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
SacI GAGCTC 1 cut(s) 398
SaqAI TTAA 2 cut(s) 387, 515
SatI GCNGC 3 cut(s) 73, 157, 232
Sau96I GGNCC 3 cut(s) 240, 265, 266
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 3 cut(s) 269, 309, 398
SfaNI GCATC 1 cut(s) 523
SfcI CTRYAG 1 cut(s) 174
SmiI ATTTAAAT 1 cut(s) 516
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
SpeI ACTAGT 1 cut(s) 506
SsiI CCGC 3 cut(s) 9, 53, 156
SspMI CTAG 2 cut(s) 168, 507
SstI GAGCTC 1 cut(s) 398
StyD4I CCNGG 1 cut(s) 324
SwaI ATTTAAAT 1 cut(s) 516
TaaI ACNGT 2 cut(s) 49, 212
TaiI ACGT 3 cut(s) 190, 315, 360
TaqI TCGA 3 cut(s) 144, 246, 413
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 2 cut(s) 387, 515
Tru9I TTAA 2 cut(s) 387, 515
TseFI GTSAC 4 cut(s) 55, 118, 424, 553
TseI GCWGC 2 cut(s) 72, 231
Tsp45I GTSAC 4 cut(s) 55, 118, 424, 553
TspGWI ACGGA 1 cut(s) 443
XspI CTAG 2 cut(s) 168, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.