AT5G02430

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012301
Physical Location & Seq
Forward (+)
526433 .. 529982
3550 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G02430.1

Sequence Viewer

Length: 2718 bp
ATGGCGAATCGAGGCGGCGAAAACGGGAAATTGGATCGGAAGAAGACGATGACGATGAATTGGGCGGGGTTAGGCGAGTTTGAAGACGACGACGATCATTTTTTCGATAGCTCTAATCGTATCTCCACCGTCGTACCTTCGGATTTAGCATCATCTTCTGACGAAGAAGAAGGTGAGTTTGATGATTGCCGGATCTCGTTTTCTTCTAATGTGTCGTCCTCCTCTCGCGCTGCTCCTGCGCCGGATATGTCGCCGGATTATGATATTTGGATGTCGGCGCCGGGATCTATCACGGAGCGTCGTCGAAGGCTTCTTAATGGAATGGGACTTGAATCTAAGAAGACTAGGCTCGGAGCTATCTCGATTCAACGCGTGAGTGAACCTGTGGCGGCGACGGGTGAGTGCTCAGGTTCTCGCGTTGCGGAAGTGAAGGTGGAGGAGTCAGATCACAATTCTCCGGTGAATCAAAGAGTTCGTCGATCTCCGTCGATGTCGGTTTTGCTTGTACGGTCTAGATCTGATTCCGATATCGAAGCGTCTTCTGCTGAGAAACAGAGGAAAGAAGAGATGTTAGGGAAAACGTCGAAATCGCGATTGACTAGAACCGCGTCTGCGATTGGAGCTCCGTGTGCGAGGATTTGTCCCTACTTCACCCAAACGCAAGCCTCGCCGCCGGACGCACCAAACGGTCATCATCAGTCTCAAGGCCAACGTAGTGGTCCGTTGCTCTCTTCGGTAGTTTCCAATACTCGTTTTAGTGCTTTTTTCTTGATTAAGAACCTTGACACTGGGAAAGAGTTCATTGTGAAGGAGTATGGTGAGAATGGAATGTGGAATCGGCTTAGCGATTTGCAAACTGGTAAGCAATTGACAATGGAAGAATTTGAGAAGAGTGTTGGTTATTCATCTGTTGTTAAGGATCTTATGAGAAGAGAGAACGCGAATTCGACGATGGATTTTAGGAAATTTAACTCGTATGTGTCAAAGAGTTTGAGAGTAAGTAAGAAGAGAGGAGCTGCTTTGTTAAAGAACATAAAGGACGTTGCTCATTCTATGAGCTCTTCTAAGGTTTCAGAGAAAGAGAAGGATTCAACTGGTAGTGGCACAAGTAGTCCAAAAGTAGCGGAGAAGAACAATGAGCAGGCGAATCAATGGGTGAAAGTGAGGCATTCAGGGAAGTCTCATAAAGATTTAAGTGCTTTGCATATGTGTCAAGAGATTCAAGCTCATCAAGGAGGTATTTGGACTATGAAATTTAGTCCAGATTCACATTTATTAGCAAGTGCAGGGGAGGATTGTGCAATCCATGTGTGGGAGGTTCAAGAATGTGAAATCATGTCAATGAATGAAGGGAGCTTGACACCTATTCATCCTTCAATGTCTGGTTCCACAGATAAATCGTCGGAAGGTGATGCTGCAGAAGTGTCACAAGATAAGAAGAAGAAAGGGAAAACTTCAATGAGTAAAAAAGGAAACCAGATACCAGATTATGTCCACGCGCCTGAAACTGTGTTCTCCTTGTCAGATAAACCAATTTGCTCTTTCACTGGTCACTTGGATGATGTTCTGGACTTATCTTGGTCTCGGTCACAGCTATTGCTTTCATCTTCCATGGACAAAACTGTGCGGTTGTGGGATATTGAAACTCAAAGTTGTCTAAAACTATTTGCCCACAATGACTATGTTACTTGTGTACAATTCAATCCATTGGATGAAGATTACTTCATTAGTGGCTCCCTTGATGCCAAGATCCGTATATGGAACATATCAAACCGTCAAGTAGTTGAGTGGAATGATCTAAAGGAAATGGTTACCGCGGTTTGTTATACTCCTGATGGTCAGGCTGCATTTGTTGGTTCAATTAATGGGCATTGCCGGCTTTACAGTGCAGAGGATTGCAAGTTGGAACAAACAAACCAGATAGATCTGCAGAACAAAAAGAAGGCTCAAGCTAAAAAGATCACAGCTTTTCAGTTTTCGCCAATTAATCCATCAGAAGTGCTTGTAACTTCTGCTGATTCACGCATTCGGGTTCTTGACGGTACTGAACTTGTTCAAAAATTCAGAGGCTTCAAAAACATGAACAGCCAAATGACAGCATCTTACACAGTAGATGCTAAGCACATTGTCTGCGCTAGCGAAGACTCACAGGTTTACGTGTGGAAACACGAGGAGCCACGACTAGGTATCACCGGTAGAAAAACAGTCACCATGTGTACTTCTTATGAAACCTTCCCGTGCAAAGATGTTTCCGTGGCAGTCCCATGGAACGGCGTTGTAAAAGGTGAACCACCATCCACACAACACCAATCGAAAAGAAACCCGAAAAAACCATCAACTACTACTACTACCCCTGAAAACACGACCACGGGTAAAAAATCAGGCCTCCCACCATTACCTAAGAAAAACAATGACGGCACCGCGGATGGAGCAACAGAACAACATCAAGAAGATGATCCAGCTACGCATATTTCGCATAACGAATCAGAGAACAACACAGGCGAGTCAATAAAGCATGGGGATTCACCTTCTATATCAATCTCCTCTCGGATTTCTTCTTGGTCGTGGTTTGATAGTAGTGGCAGTCATGGAACACATTCAGTTCAGCCAACAGCTTGGGGAATGGTGATTGTTACAGCCACAATCAAGGGCCAAATCCGAACTTACCAAAATTTTGGATTACCACGCAGGGTCGGTCGTCAAGGCTCTCTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

905

Amino Acids

99.89

Weight (kDa)

7.33

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 399 - 448 9e-07 THOC3 beta-propeller domain
WD40 PF00400 405 - 438 2.2e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 405 - 446 3.1e-06 WDR5 beta-propeller domain
Beta-prop_WDR5 PF25175 509 - 728 1e-39 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 509 - 589 2.1e-14 WDR3 first beta-propeller domain
WD40 PF00400 510 - 546 4.3e-07 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 510 - 572 9.2e-08 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 510 - 631 9.9e-15 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 511 - 677 5.6e-14 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 514 - 724 1.5e-25 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 515 - 572 2.2e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 516 - 591 6.9e-07 TEP-1 second beta-propeller
Beta-prop_RIG_2nd PF23775 520 - 579 9e-06 RIG second beta-propeller
Beta-prop_Aladin PF25460 520 - 652 1.3e-11 Aladin seven-bladed propeller
Beta-prop_WDR3_2nd PF25172 521 - 721 8.9e-14 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 522 - 722 8.2e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta PF25391 532 - 629 1.2e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 533 - 634 9.1e-11 WDR90/POC16, second beta-propeller
WD40_MABP1-WDR62_1st PF24780 539 - 639 7.8e-07 MABP1/WDR62 first WD40 domain
WD40_WDHD1_1st PF24817 561 - 724 2.8e-13 WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 571 - 654 9.5e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2 PF23414 572 - 725 6.1e-16 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 586 - 723 2.2e-08 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37670 AT2G37670 AT5G02430
fragaria_vesca FvH4_6g13960
malus_domestica MD04G1127300.v1.1 MD12G1140500.v1.1
prunus_persica Prupe.6G253900_v2.0.a1 Prupe.6G253900_v2.0.a1
pyrus_communis pycom04g11410
rosa_chinensis RchiOBHm_Chr3g0466781
rosa_laevigata RLG00000024553
rosa_multiflora Rmu_sc0008017.1_g000009
rosa_roxburghii Rroxscaffold_6G00414170
rosa_rugosa Rorug03G0083800
rosa_samantha Rh3AG131000 Rh3BG152000 Rh3CG151900 Rh3DG152700
rosa_wichuraiana Rw3G012380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 277, 2417
AccII CGCG 9 cut(s) 228, 372, 417, 592, 608, 941, 1499, 1817, 2423
AclWI GGATC 6 cut(s) 42, 200, 292, 927, 1744, 2450
AcsI RAATTY 6 cut(s) 881, 943, 965, 1253, 2060, 2671
AcyI GRCGYC 1 cut(s) 278
AdeI CACNNNGTG 1 cut(s) 2214
AfaI GTAC 5 cut(s) 135, 507, 1695, 2044, 2218
AfiI CCNNNNNNNGG 3 cut(s) 1312, 2183, 2270
AflIII ACRYGT 2 cut(s) 370, 2157
AgeI ACCGGT 1 cut(s) 2192
Alw21I GWGCWC 3 cut(s) 407, 625, 1061
Alw26I GTCTC 3 cut(s) 705, 1185, 1587
AlwI GGATC 6 cut(s) 42, 200, 292, 927, 1744, 2450
AoxI GGCC 3 cut(s) 706, 2383, 2650
ApeKI GCWGC 4 cut(s) 230, 1016, 1415, 1844
ApoI RAATTY 6 cut(s) 881, 943, 965, 1253, 2060, 2671
ArsI GACNNNNNNTTYG 2 cut(s) 460, 492
AseI ATTAAT 2 cut(s) 1863, 1986
AsiGI ACCGGT 1 cut(s) 2192
Asp700I GAANNNNTTC 3 cut(s) 797, 2052, 2596
AspLEI GCGC 5 cut(s) 230, 241, 280, 1501, 2135
AspS9I GGNCC 2 cut(s) 719, 2650
AsuC2I CCSGG 1 cut(s) 282
AsuNHI GCTAGC 1 cut(s) 2135
AvaII GGWCC 1 cut(s) 719
BaeI ACNNNNGTAYC 2 cut(s) 117, 150
BanI GGYRCC 2 cut(s) 277, 2417
BanII GRGCYC 2 cut(s) 625, 1061
BauI CACGAG 1 cut(s) 2168
BbsI GAAGAC 5 cut(s) 50, 90, 347, 531, 2148
Bbv12I GWGCWC 3 cut(s) 407, 625, 1061
BbvI GCAGC 4 cut(s) 217, 1003, 1402, 1831
BccI CCATC 6 cut(s) 946, 1829, 1999, 2302, 2341, 2420
BceAI ACGGC 2 cut(s) 2287, 2431
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 3 cut(s) 705, 1185, 1587
BfaI CTAG 5 cut(s) 345, 513, 600, 2136, 2183
BfmI CTRYAG 2 cut(s) 1416, 1928
BfoI RGCGCY 1 cut(s) 281
BglII AGATCT 2 cut(s) 515, 1924
BisI GCNGC 7 cut(s) 16, 231, 390, 671, 1017, 1416, 1845
BlpI GCTNAGC 2 cut(s) 842, 2118
BlsI GCNGC 7 cut(s) 17, 232, 391, 672, 1018, 1417, 1846
Bme1390I CCNGG 1 cut(s) 282
Bme18I GGWCC 1 cut(s) 719
BmgT120I GGNCC 2 cut(s) 719, 2650
BmiI GGNNCC 5 cut(s) 279, 1387, 1735, 2175, 2419
BmrFI CCNGG 1 cut(s) 282
BmrI ACTGGG 1 cut(s) 798
BmsI GCATC 5 cut(s) 158, 1402, 1732, 2104, 2108
BmtI GCTAGC 1 cut(s) 2139
BmuI ACTGGG 1 cut(s) 798
BpiI GAAGAC 5 cut(s) 50, 90, 347, 531, 2148
Bpu10I CCTNAGC 1 cut(s) 406
Bpu1102I GCTNAGC 2 cut(s) 842, 2118
BpuEI CTTGAG 2 cut(s) 687, 1932
BpuMI CCSGG 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 2158
BsaHI GRCGYC 1 cut(s) 278
BsaI GGTCTC 1 cut(s) 1587
BsaJI CCNNGG 6 cut(s) 1611, 1815, 2253, 2264, 2367, 2421
BsaWI WCCGGW 2 cut(s) 457, 2192
Bsc4I CCNNNNNNNGG 3 cut(s) 1312, 2183, 2270
Bse118I RCCGGY 2 cut(s) 1875, 2192
Bse1I ACTGG 4 cut(s) 793, 862, 1099, 1552
Bse3DI GCAATG 1 cut(s) 1870
BseDI CCNNGG 6 cut(s) 1611, 1815, 2253, 2264, 2367, 2421
BseGI GGATG 6 cut(s) 276, 1369, 1564, 1717, 2294, 2431
BseLI CCNNNNNNNGG 3 cut(s) 1312, 2183, 2270
BseMI GCAATG 1 cut(s) 1870
BseMII CTCAG 2 cut(s) 420, 537
BseNI ACTGG 4 cut(s) 793, 862, 1099, 1552
BseRI GAGGAG 5 cut(s) 211, 452, 1026, 2186, 2533
BseXI GCAGC 4 cut(s) 217, 1003, 1402, 1831
BsgI GTGCAG 2 cut(s) 1305, 1908
Bsh1236I CGCG 9 cut(s) 228, 372, 417, 592, 608, 941, 1499, 1817, 2423
Bsh1285I CGRYCG 1 cut(s) 2698
BshFI GGCC 3 cut(s) 708, 2385, 2652
BshNI GGYRCC 2 cut(s) 277, 2417
BshTI ACCGGT 1 cut(s) 2192
BsiEI CGRYCG 1 cut(s) 2698
BsiHKAI GWGCWC 3 cut(s) 407, 625, 1061
BsiSI CCGG 8 cut(s) 190, 242, 254, 281, 458, 674, 1876, 2193
BslFI GGGAC 3 cut(s) 339, 627, 2246
BslI CCNNNNNNNGG 3 cut(s) 1312, 2183, 2270
BsmAI GTCTC 3 cut(s) 705, 1185, 1587
BsmFI GGGAC 3 cut(s) 339, 627, 2246
BsmI GAATGC 2 cut(s) 1168, 2025
BsnI GGCC 3 cut(s) 708, 2385, 2652
Bso31I GGTCTC 1 cut(s) 1587
Bsp1286I GDGCHC 3 cut(s) 407, 625, 1061
Bsp1407I TGTACA 1 cut(s) 1693
Bsp1720I GCTNAGC 2 cut(s) 842, 2118
Bsp19I CCATGG 2 cut(s) 1611, 2264
Bsp68I TCGCGA 1 cut(s) 592
BspANI GGCC 3 cut(s) 708, 2385, 2652
BspCNI CTCAG 2 cut(s) 419, 538
BspFNI CGCG 9 cut(s) 228, 372, 417, 592, 608, 941, 1499, 1817, 2423
BspLI GGNNCC 5 cut(s) 279, 1387, 1735, 2175, 2419
BspMAI CTGCAG 2 cut(s) 1420, 1932
BspOI GCTAGC 1 cut(s) 2139
BspPI GGATC 6 cut(s) 42, 200, 292, 927, 1744, 2450
BspQI GCTCTTC 1 cut(s) 1066
BspT107I GGYRCC 2 cut(s) 277, 2417
BspTNI GGTCTC 1 cut(s) 1587
BsrDI GCAATG 1 cut(s) 1870
BsrFI RCCGGY 2 cut(s) 1875, 2192
BsrGI TGTACA 1 cut(s) 1693
BsrI ACTGG 4 cut(s) 793, 862, 1099, 1552
BssAI RCCGGY 2 cut(s) 1875, 2192
BssECI CCNNGG 6 cut(s) 1611, 1815, 2253, 2264, 2367, 2421
BssNI GRCGYC 1 cut(s) 278
BssSI CACGAG 1 cut(s) 2168
BssT1I CCWWGG 2 cut(s) 1611, 2264
Bst2BI CACGAG 1 cut(s) 2168
Bst6I CTCTTC 6 cut(s) 558, 736, 884, 925, 1001, 1066
BstACI GRCGYC 1 cut(s) 278
BstAUI TGTACA 1 cut(s) 1693
BstBAI YACGTR 1 cut(s) 2158
BstC8I GCNNGC 4 cut(s) 663, 1143, 1877, 2137
BstDEI CTNAG 7 cut(s) 336, 406, 546, 842, 1065, 2118, 2400
BstDSI CCRYGG 6 cut(s) 1611, 1815, 2253, 2264, 2367, 2421
BstEII GGTNACC 1 cut(s) 1810
BstF5I GGATG 6 cut(s) 276, 1369, 1564, 1717, 2294, 2431
BstFNI CGCG 9 cut(s) 228, 372, 417, 592, 608, 941, 1499, 1817, 2423
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 5 cut(s) 230, 241, 280, 1501, 2135
BstMAI GTCTC 3 cut(s) 705, 1185, 1587
BstMCI CGRYCG 1 cut(s) 2698
BstMWI GCNNNNNNNGC 8 cut(s) 236, 542, 620, 629, 667, 1876, 2429, 2473
BstPI GGTNACC 1 cut(s) 1810
BstSCI CCNGG 1 cut(s) 280
BstSFI CTRYAG 2 cut(s) 1416, 1928
BstUI CGCG 9 cut(s) 228, 372, 417, 592, 608, 941, 1499, 1817, 2423
BstV1I GCAGC 4 cut(s) 217, 1003, 1402, 1831
BstV2I GAAGAC 5 cut(s) 50, 90, 347, 531, 2148
BstX2I RGATCY 6 cut(s) 192, 284, 515, 919, 1749, 1924
BstXI CCANNNNNNTGG 3 cut(s) 716, 2616, 2675
BstYI RGATCY 6 cut(s) 192, 284, 515, 919, 1749, 1924
BsuRI GGCC 3 cut(s) 708, 2385, 2652
BtgI CCRYGG 6 cut(s) 1611, 1815, 2253, 2264, 2367, 2421
BtsCI GGATG 6 cut(s) 276, 1369, 1564, 1717, 2294, 2431
BtsIMutI CAGTG 3 cut(s) 786, 1545, 1891
BtuMI TCGCGA 1 cut(s) 592
Cac8I GCNNGC 4 cut(s) 663, 1143, 1877, 2137
CfoI GCGC 5 cut(s) 230, 241, 280, 1501, 2135
Cfr10I RCCGGY 2 cut(s) 1875, 2192
Cfr13I GGNCC 2 cut(s) 719, 2650
Cfr42I CCGCGG 2 cut(s) 1818, 2424
CseI GACGC 4 cut(s) 287, 525, 597, 686
Csp6I GTAC 5 cut(s) 134, 506, 1694, 2043, 2217
CspAI ACCGGT 1 cut(s) 2192
CviAII CATG 8 cut(s) 1307, 1336, 1612, 2080, 2212, 2265, 2516, 2588
CviQI GTAC 5 cut(s) 134, 506, 1694, 2043, 2217
DdeI CTNAG 7 cut(s) 336, 406, 546, 842, 1065, 2118, 2400
DinI GGCGCC 1 cut(s) 279
DraIII CACNNNGTG 1 cut(s) 2214
Eam1104I CTCTTC 6 cut(s) 558, 736, 884, 925, 1001, 1066
EarI CTCTTC 6 cut(s) 558, 736, 884, 925, 1001, 1066
Ecl136II GAGCTC 2 cut(s) 623, 1059
Eco130I CCWWGG 2 cut(s) 1611, 2264
Eco147I AGGCCT 1 cut(s) 2385
Eco24I GRGCYC 2 cut(s) 625, 1061
Eco31I GGTCTC 1 cut(s) 1587
Eco32I GATATC 1 cut(s) 529
Eco47I GGWCC 1 cut(s) 719
Eco53kI GAGCTC 2 cut(s) 623, 1059
Eco91I GGTNACC 1 cut(s) 1810
EcoICRI GAGCTC 2 cut(s) 623, 1059
EcoO65I GGTNACC 1 cut(s) 1810
EcoRI GAATTC 1 cut(s) 943
EcoRV GATATC 1 cut(s) 529
EcoT14I CCWWGG 2 cut(s) 1611, 2264
EcoT38I GRGCYC 2 cut(s) 625, 1061
EgeI GGCGCC 1 cut(s) 279
EheI GGCGCC 1 cut(s) 279
ErhI CCWWGG 2 cut(s) 1611, 2264
FaeI CATG 8 cut(s) 1310, 1339, 1615, 2083, 2215, 2268, 2519, 2591
FaqI GGGAC 3 cut(s) 339, 627, 2246
FatI CATG 8 cut(s) 1306, 1335, 1611, 2079, 2211, 2264, 2515, 2587
FauI CCCGC 1 cut(s) 58
FauNDI CATATG 1 cut(s) 1206
Fnu4HI GCNGC 7 cut(s) 16, 231, 390, 671, 1017, 1416, 1845
FokI GGATG 6 cut(s) 283, 1356, 1571, 1724, 2281, 2438
FriOI GRGCYC 2 cut(s) 625, 1061
Fsp4HI GCNGC 7 cut(s) 16, 231, 390, 671, 1017, 1416, 1845
FspBI CTAG 5 cut(s) 345, 513, 600, 2136, 2183
GlaI GCGC 5 cut(s) 229, 240, 279, 1500, 2134
GluI GCNGC 7 cut(s) 16, 231, 390, 671, 1017, 1416, 1845
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 3 cut(s) 708, 2385, 2652
HapII CCGG 8 cut(s) 190, 242, 254, 281, 458, 674, 1876, 2193
HgaI GACGC 4 cut(s) 287, 525, 597, 686
HhaI GCGC 5 cut(s) 230, 241, 280, 1501, 2135
Hin1I GRCGYC 1 cut(s) 278
Hin1II CATG 8 cut(s) 1310, 1339, 1615, 2083, 2215, 2268, 2519, 2591
Hin6I GCGC 5 cut(s) 228, 239, 278, 1499, 2133
HinP1I GCGC 5 cut(s) 228, 239, 278, 1499, 2133
HpaII CCGG 8 cut(s) 190, 242, 254, 281, 458, 674, 1876, 2193
Hpy166II GTNNAC 6 cut(s) 380, 1495, 1694, 2155, 2217, 2288
Hpy8I GTNNAC 6 cut(s) 380, 1495, 1694, 2155, 2217, 2288
HpyCH4IV ACGT 4 cut(s) 581, 712, 1041, 2157
HpyF10VI GCNNNNNNNGC 8 cut(s) 236, 542, 620, 629, 667, 1876, 2429, 2473
HpyF3I CTNAG 7 cut(s) 336, 406, 546, 842, 1065, 2118, 2400
HpySE526I ACGT 4 cut(s) 581, 712, 1041, 2157
Hsp92I GRCGYC 1 cut(s) 278
Hsp92II CATG 8 cut(s) 1310, 1339, 1615, 2083, 2215, 2268, 2519, 2591
HspAI GCGC 5 cut(s) 228, 239, 278, 1499, 2133
KasI GGCGCC 1 cut(s) 277
KroI GCCGGC 1 cut(s) 1875
KroNI GCCGGC 1 cut(s) 1877
KspI CCGCGG 2 cut(s) 1818, 2424
LguI GCTCTTC 1 cut(s) 1066
Lsp1109I GCAGC 4 cut(s) 217, 1003, 1402, 1831
LweI GCATC 5 cut(s) 158, 1402, 1732, 2104, 2108
MaeI CTAG 5 cut(s) 345, 513, 600, 2136, 2183
MaeII ACGT 4 cut(s) 581, 712, 1041, 2157
MaeIII GTNAC 8 cut(s) 1425, 1550, 1587, 1684, 1810, 2005, 2206, 2632
MfeI CAATTG 1 cut(s) 866
MflI RGATCY 6 cut(s) 192, 284, 515, 919, 1749, 1924
MhlI GDGCHC 3 cut(s) 407, 625, 1061
MluI ACGCGT 1 cut(s) 370
Mly113I GGCGCC 1 cut(s) 278
MlyI GAGTC 3 cut(s) 449, 2138, 2513
MmeI TCCRAC 2 cut(s) 1383, 1884
MroNI GCCGGC 1 cut(s) 1875
MroXI GAANNNNTTC 3 cut(s) 797, 2052, 2596
MseI TTAA 8 cut(s) 315, 774, 915, 969, 1025, 1193, 1863, 1986
MslI CAYNNNNRTG 2 cut(s) 1340, 1557
MspA1I CMGCKG 2 cut(s) 1817, 2423
MspI CCGG 8 cut(s) 190, 242, 254, 281, 458, 674, 1876, 2193
MspR9I CCNGG 1 cut(s) 282
MunI CAATTG 1 cut(s) 866
Mva1269I GAATGC 2 cut(s) 1168, 2025
MvnI CGCG 9 cut(s) 228, 372, 417, 592, 608, 941, 1499, 1817, 2423
MwoI GCNNNNNNNGC 8 cut(s) 236, 542, 620, 629, 667, 1876, 2429, 2473
NaeI GCCGGC 1 cut(s) 1877
NarI GGCGCC 1 cut(s) 278
NciI CCSGG 1 cut(s) 282
NcoI CCATGG 2 cut(s) 1611, 2264
NdeI CATATG 1 cut(s) 1206
NgoMIV GCCGGC 1 cut(s) 1875
NheI GCTAGC 1 cut(s) 2135
NlaIII CATG 8 cut(s) 1310, 1339, 1615, 2083, 2215, 2268, 2519, 2591
NlaIV GGNNCC 5 cut(s) 279, 1387, 1735, 2175, 2419
NmuCI GTSAC 4 cut(s) 1425, 1550, 1587, 2206
NruI TCGCGA 1 cut(s) 592
PceI AGGCCT 1 cut(s) 2385
PciSI GCTCTTC 1 cut(s) 1066
PctI GAATGC 2 cut(s) 1168, 2025
PdiI GCCGGC 1 cut(s) 1877
PdmI GAANNNNTTC 3 cut(s) 797, 2052, 2596
PinAI ACCGGT 1 cut(s) 2192
PkrI GCNGC 7 cut(s) 17, 232, 391, 672, 1018, 1417, 1846
PleI GAGTC 3 cut(s) 448, 2138, 2512
PluTI GGCGCC 1 cut(s) 281
PpsI GAGTC 3 cut(s) 448, 2138, 2512
Ppu21I YACGTR 1 cut(s) 2158
PshBI ATTAAT 2 cut(s) 1863, 1986
Psp124BI GAGCTC 2 cut(s) 625, 1061
PspEI GGTNACC 1 cut(s) 1810
PspN4I GGNNCC 5 cut(s) 279, 1387, 1735, 2175, 2419
PspPI GGNCC 2 cut(s) 719, 2650
PstI CTGCAG 2 cut(s) 1420, 1932
PsuI RGATCY 6 cut(s) 192, 284, 515, 919, 1749, 1924
RruI TCGCGA 1 cut(s) 592
RsaI GTAC 5 cut(s) 135, 507, 1695, 2044, 2218
RsaNI GTAC 5 cut(s) 134, 506, 1694, 2043, 2217
RseI CAYNNNNRTG 2 cut(s) 1340, 1557
SacI GAGCTC 2 cut(s) 625, 1061
SacII CCGCGG 2 cut(s) 1818, 2424
SapI GCTCTTC 1 cut(s) 1066
SaqAI TTAA 8 cut(s) 315, 774, 915, 969, 1025, 1193, 1863, 1986
SatI GCNGC 7 cut(s) 16, 231, 390, 671, 1017, 1416, 1845
Sau96I GGNCC 2 cut(s) 719, 2650
SchI GAGTC 3 cut(s) 449, 2138, 2513
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 3 cut(s) 407, 625, 1061
SfaNI GCATC 5 cut(s) 158, 1402, 1732, 2104, 2108
SfcI CTRYAG 2 cut(s) 1416, 1928
SfoI GGCGCC 1 cut(s) 279
Sfr303I CCGCGG 2 cut(s) 1818, 2424
SgrBI CCGCGG 2 cut(s) 1818, 2424
SinI GGWCC 1 cut(s) 719
SmiMI CAYNNNNRTG 2 cut(s) 1340, 1557
SmlI CTYRAG 2 cut(s) 702, 1947
SmoI CTYRAG 2 cut(s) 702, 1947
SseBI AGGCCT 1 cut(s) 2385
SspDI GGCGCC 1 cut(s) 277
SspMI CTAG 5 cut(s) 345, 513, 600, 2136, 2183
SstI GAGCTC 2 cut(s) 625, 1061
StuI AGGCCT 1 cut(s) 2385
StyD4I CCNGG 1 cut(s) 280
StyI CCWWGG 2 cut(s) 1611, 2264
TaiI ACGT 4 cut(s) 584, 715, 1044, 2160
TaqII GACCGA 2 cut(s) 1575, 2684
TatI WGTACW 2 cut(s) 1693, 2216
TauI GCSGC 3 cut(s) 18, 392, 673
Tru1I TTAA 8 cut(s) 315, 774, 915, 969, 1025, 1193, 1863, 1986
Tru9I TTAA 8 cut(s) 315, 774, 915, 969, 1025, 1193, 1863, 1986
TscAI CASTG 3 cut(s) 793, 1552, 1891
TseFI GTSAC 4 cut(s) 1425, 1550, 1587, 2206
TseI GCWGC 4 cut(s) 230, 1016, 1415, 1844
Tsp45I GTSAC 4 cut(s) 1425, 1550, 1587, 2206
TspGWI ACGGA 6 cut(s) 308, 474, 615, 711, 1742, 2242
TspRI CASTG 3 cut(s) 793, 1552, 1891
VpaK11BI GGWCC 1 cut(s) 719
VspI ATTAAT 2 cut(s) 1863, 1986
XapI RAATTY 6 cut(s) 881, 943, 965, 1253, 2060, 2671
XbaI TCTAGA 1 cut(s) 512
XmnI GAANNNNTTC 3 cut(s) 797, 2052, 2596
XspI CTAG 5 cut(s) 345, 513, 600, 2136, 2183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.