Rw3G012380

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0012301
Physical Location & Seq
Forward (+)
12184982 .. 12188768
3787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G012380.1

Sequence Viewer

Length: 2871 bp
ATGATCATGGAGCCGAAGATGGAGAGGAAGAGGACGTTCGCTATGAACTGGGACGGCCTCCACGACGAAGAAGACGACGACCGCTTCTTCGAGTCCCGGGAGAGAATGTCCTCCGCCGTGCCTATCGACCTCGAAGCCTCCTCCTCCGACGATGAGGACTTCGACGACAGTCGTATGTCCTTCGCCTCCTGCGTCTCCTCAGTCCGAAACGACGTCGAGGTGCTGCGAGCCTTGTCGGCCGCCGAGCCGTCCTTCGCCTCGTCGCCTCCGATGTCACCGGACTACAACATCTGGATGGCCGCGCCGGGGTCGATCTCCGAACGCCGCAGGCGGTTGTTGCAGGGCATGGGATTGGCCAACGGCGGCAAAGACATCGTCAGCTTCAGACGCCTCGTTTCGAATAAAGCCCCAAACGGCGTCGTTCCGGCAGTTACGAGTTGCCCCACTCGCTCTCGGGAAAAGATGCAAGTTCCCGAAACGGCGCCTGAACCCGATCCCGATCCGGAGCCAGAGTCGGGGGCTTCGAATTCGCCGTTGCCGATGATGCTTGTACGGTCCAGATCGGAAGGAGAGATCGAAGCTTTCTCGGCCGATAAACGAAGGAAAGAGGAGGTAATTGGCACTGTCTCGAAACAACGGCTGACAAGAACGTCGTCGGCGATCGCGGCGCCTAGCTCTAGGATGTGTTTTTCCACGACGCCTCAAAGCGAAGGGGGAACCAAGACAGTCCGATCTAGGCAAAACAGCGCCGCATTGTCGACGGAGTTCACCAACAATTTAGGGGCGTTCTTCTTGATTAAAAATCTGGACACGGGAACCGAGTTCATTGTGAACGAGTACGATCAAAACGGGTGTTGGAACAAGTTTAGTGATCTCCAAACGGGGAAGAAATTGACCGTGGACGAGTTTGAGAAGTCCGTCGGGTACTCGCCGGTGGTGAAGGAGGTGATGCGGCGGCAAAATGTCAACATTGAAGCCGGGATTGAGAGGAAGGTACCAGCAGGAGCCAATTCGTTTGTGTCAAAGAGTTTGAGGATGAGCAAGAGAAAGGTGGCTCTTTTGAAGAATAGCATAAAAGGTGTGGGAAGTGCTGTGAGTGTACTAATTGGGGATAAGGATCAACACGCCTCGTCGCCGCCGCCGCCGCCGCTGCCGCAGGACCAGAAATCAAGCAAGGCTAATTCGGGGTCTGGTTCGGAGTGGATCAAAGTTAGGCAGACTGGGAAGTCATACAAGGAGCTATCAGCATTGCATTTGTGCCAGGAGATTCAGGCTCATGAGGGCTCAATTTGGACGATCAAGTTTAGCTTGGATGCTAGATTTCTTGCTAGTGCTGGAGAGGACAGGGTCATTCATGTATGGGAAGTTCAAGAATGTGAGATTATGTCCATGGATAGCAATGCGAATCCGCTGCATCCCTCCTCGGTGTCCCCATATCCGGATCGATCAATGTGTCTTAGCTCTGAAGCTTCAATTTCCTCTGAGAAGAAGAAGAAGGGGAAGGGAGGCTCTTCTAGGAAAACTAATCCCATTCCTGACTATGTCCATGTACCCGAATTCGTGTTTGCCCTCTCGGAGAAACCTGTGTGCTCTTTCGAAGGTCATTTGGATGCAGTACTGGACCTGTCATGGTCCAGAGATCAGCTATTGCTTTCATCTTCAATGGACAAAACTGTTAGATTATGGGATATGGTGACCAAGAGTTGTTTAAAGATGTTTGCCCACAATGATTATGTAACTTGCATACAGTTCAATCCATTGGACGACAGTGTTTTTCTCAGCGGCTCCCTTGATGCGAAGCTTCGATTATGGGACATATCTGATCGAAGAGTGGTGGACTGGACTGATCTTCATGAAATGGTCACCGCTGCATGCTATACTCCAGATGGCCAGGCTGCCATCATTGGCTCGCACAAAGGGAACTGTCGTATGTACAATGCAGAAGATTGTAAATTAAGTCAAGGAGGCCAGATTGATATTAAAAACAAGAAGAAGAATCAAGCAAAGAAAATTACCGGTTTCCAGTTTTCTCCGATTAATCCATCTGAAATGCTTGTCACATCTGCTGATTCCCGGATTAGGATTGTGGACGGTAAAGATGTCACTCATAAGTTTAGAGGTTTCCGAAATACGAGCAGTCAAATTGGAGCGTCATTCACTCCCAATGGAAAGTACATTATATGTGCCAGTGAAGATTCTCATGTATATGTATGGAAGCGTGAAGAGCCTCGAGTGTCAGCTGCAGGAAAAAAGAGCACAATCACCACCACTTCTCATGAATATTTTCAGTGTAAAGATGTTTCAGTAGCAATCCCATGGCCTGGCACCATAAAAGGTGACCCTCCCCCGCCTGTGTCTGTGCATTCTTCTAAGAGGCACTCAAAACGTTCCTCTGTACAGCCAGTTTCAGGAACTGCATCACCTACTCAAGACGGAGGAGACAGCAAGAGATTCCTGCCACCTCTTCCAAAGAAAAACACTAATCCTAGTACTCCTCCGAGTGCAATAGCGGAGAAAACCACTAATCTTAGTACTCCTCCTAATGCGACAGCGGAGAAACCTTCAACTCCCCCACCAGAAGATCAGGATCCATCTGAAATTTCTCGAACGGATTCTGGGATTGGCGAGTCATTCAGCTCAGACCCTTCCTCTATTAGGTATGGTGGCGATTCACCTTCTATATCTTCTGGCAATGCATCATCATCTTGGTCTTCCTCTTGGTCCTCTTTGTTTGGGCATGAATCGAGCCAAACAACCCAAGCTTGGGGCTTGGTCATTGTGACAGCCACCCTGGAAGGTGAAATCAGGGCATATCAAAATTTTGGGATACCAAGAAGGGTGCCCGGCAGTTTTTTTGGAGGTCCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

956

Amino Acids

104.66

Weight (kDa)

7.53

Isoelectric Point (pI)

56.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 415 - 462 4.7e-07 THOC3 beta-propeller domain
WD40 PF00400 420 - 454 7.7e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 525 - 648 3.2e-10 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 526 - 646 5e-17 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 526 - 756 2.3e-19 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 526 - 589 1.8e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 527 - 747 8.6e-38 WDR5 beta-propeller domain
WD40 PF00400 527 - 563 6.9e-08 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 528 - 622 4.3e-10 CDC20/Fizzy WD40 domain
EIF3I PF24805 529 - 728 2.1e-10 EIF3I
Beta-prop_THOC3 PF25174 530 - 572 1.7e-06 THOC3 beta-propeller domain
Beta-prop_IFT122_1st PF23381 532 - 646 1.1e-06 IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 532 - 612 1.1e-10 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 535 - 738 3e-08 WDR55
Beta-prop_THOC3 PF25174 535 - 742 9.6e-27 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 537 - 652 9.3e-10 Aladin seven-bladed propeller
WD40_Gbeta PF25391 546 - 646 3.6e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 550 - 650 1.6e-07 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 578 - 744 6.9e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 590 - 755 2.2e-15 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37670 AT2G37670 AT5G02430
fragaria_vesca FvH4_6g13960
malus_domestica MD04G1127300.v1.1 MD12G1140500.v1.1
prunus_persica Prupe.6G253900_v2.0.a1 Prupe.6G253900_v2.0.a1
pyrus_communis pycom04g11410
rosa_chinensis RchiOBHm_Chr3g0466781
rosa_laevigata RLG00000024553
rosa_multiflora Rmu_sc0008017.1_g000009
rosa_roxburghii Rroxscaffold_6G00414170
rosa_rugosa Rorug03G0083800
rosa_samantha Rh3AG131000 Rh3BG152000 Rh3CG151900 Rh3DG152700
rosa_wichuraiana Rw3G012380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 649, 1225
AatII GACGTC 1 cut(s) 216
Acc65I GGTACC 1 cut(s) 994
AccB1I GGYRCC 5 cut(s) 481, 667, 994, 2324, 2839
AccB7I CCANNNNNTGG 1 cut(s) 2321
AccI GTMKAC 1 cut(s) 758
AccII CGCG 2 cut(s) 302, 665
AccIII TCCGGA 2 cut(s) 502, 1438
AclI AACGTT 1 cut(s) 2386
AclWI GGATC 7 cut(s) 488, 494, 1125, 1211, 1449, 2582, 2595
AcoI YGGCCR 5 cut(s) 237, 297, 354, 588, 1888
AcsI RAATTY 4 cut(s) 526, 1556, 2598, 2818
AcuI CTGAAG 2 cut(s) 367, 1485
AcyI GRCGYC 6 cut(s) 213, 388, 417, 482, 668, 698
AfiI CCNNNNNNNGG 9 cut(s) 306, 515, 1438, 2079, 2321, 2408, 2655, 2763, 2764
AgeI ACCGGT 1 cut(s) 2015
AgsI TTSAA 7 cut(s) 974, 1063, 1370, 1473, 1662, 1753, 2565
AjnI CCWGG 4 cut(s) 1260, 1890, 2320, 2790
Alw21I GWGCWC 2 cut(s) 1592, 2258
Alw26I GTCTC 3 cut(s) 199, 631, 2433
AlwI GGATC 7 cut(s) 488, 494, 1125, 1211, 1449, 2582, 2595
AlwNI CAGNNNCTG 1 cut(s) 2414
Ama87I CYCGRG 3 cut(s) 96, 453, 2229
Aor13HI TCCGGA 2 cut(s) 502, 1438
AoxI GGCC 8 cut(s) 55, 237, 297, 354, 588, 1888, 1966, 2318
ApeKI GCWGC 6 cut(s) 223, 1150, 1411, 1868, 1895, 2240
ApoI RAATTY 4 cut(s) 526, 1556, 2598, 2818
ArsI GACNNNNNNTTYG 4 cut(s) 71, 103, 360, 392
AseI ATTAAT 1 cut(s) 2037
AsiGI ACCGGT 1 cut(s) 2015
AsiSI GCGATCGC 1 cut(s) 663
Asp700I GAANNNNTTC 2 cut(s) 2283, 2611
Asp718I GGTACC 1 cut(s) 994
AspLEI GCGC 4 cut(s) 304, 484, 670, 749
AspS9I GGNCC 6 cut(s) 555, 1159, 1621, 1632, 2721, 2861
AsuC2I CCSGG 6 cut(s) 97, 98, 306, 979, 2074, 2844
AsuII TTCGAA 3 cut(s) 398, 524, 1596
AvaI CYCGRG 3 cut(s) 96, 453, 2229
AvaII GGWCC 6 cut(s) 555, 1159, 1621, 1632, 2721, 2861
BaeGI GKGCMC 1 cut(s) 2844
BalI TGGCCA 2 cut(s) 356, 1890
BamHI GGATCC 1 cut(s) 2587
BanI GGYRCC 5 cut(s) 481, 667, 994, 2324, 2839
BanII GRGCYC 1 cut(s) 1286
BbsI GAAGAC 2 cut(s) 78, 2703
Bbv12I GWGCWC 2 cut(s) 1592, 2258
BbvI GCAGC 6 cut(s) 210, 1137, 1398, 1855, 1882, 2227
BccI CCATC 6 cut(s) 13, 289, 1880, 1907, 2050, 2599
BceAI ACGGC 8 cut(s) 70, 101, 232, 376, 430, 495, 517, 653
BcgI CGANNNNNNTGC 4 cut(s) 355, 389, 1393, 1427
BciT130I CCWGG 4 cut(s) 1262, 1892, 2322, 2792
BciVI GTATCC 1 cut(s) 2820
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 6 cut(s) 97, 98, 306, 979, 2074, 2844
BcoDI GTCTC 3 cut(s) 199, 631, 2433
BfaI CTAG 8 cut(s) 672, 678, 735, 1317, 1329, 1515, 2487, 2869
BfmI CTRYAG 1 cut(s) 2241
BfoI RGCGCY 3 cut(s) 485, 671, 750
BfuI GTATCC 1 cut(s) 2820
BglI GCCNNNNNGGC 1 cut(s) 236
BmcAI AGTACT 3 cut(s) 1617, 2491, 2533
Bme18I GGWCC 6 cut(s) 555, 1159, 1621, 1632, 2721, 2861
BmeT110I CYCGRG 3 cut(s) 96, 453, 2229
BmgT120I GGNCC 6 cut(s) 555, 1159, 1621, 1632, 2721, 2861
BmrI ACTGGG 2 cut(s) 58, 1230
BmsI GCATC 9 cut(s) 453, 534, 939, 1303, 1423, 1600, 1783, 2426, 2705
BmuI ACTGGG 2 cut(s) 58, 1230
BoxI GACNNNNGTC 1 cut(s) 168
BpiI GAAGAC 2 cut(s) 78, 2703
BpmI CTGGAG 2 cut(s) 1356, 1866
Bpu14I TTCGAA 3 cut(s) 398, 524, 1596
BpuEI CTTGAG 1 cut(s) 2412
BpuMI CCSGG 6 cut(s) 97, 98, 306, 979, 2074, 2844
Bsa29I ATCGAT 1 cut(s) 1444
BsaBI GATNNNNATC 3 cut(s) 498, 1116, 2586
BsaHI GRCGYC 6 cut(s) 213, 388, 417, 482, 668, 698
BsaJI CCNNGG 7 cut(s) 96, 305, 897, 1389, 1422, 2315, 2790
BsaWI WCCGGW 4 cut(s) 277, 502, 1438, 2015
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 497, 527
Bsc4I CCNNNNNNNGG 9 cut(s) 306, 515, 1438, 2079, 2321, 2408, 2655, 2763, 2764
Bse118I RCCGGY 2 cut(s) 931, 2015
Bse1I ACTGG 7 cut(s) 53, 1225, 1623, 1844, 2023, 2187, 2402
Bse3DI GCAATG 3 cut(s) 1247, 1405, 2698
Bse8I GATNNNNATC 3 cut(s) 498, 1116, 2586
BseAI TCCGGA 2 cut(s) 502, 1438
BseBI CCWGG 4 cut(s) 1262, 1892, 2322, 2792
BseCI ATCGAT 1 cut(s) 1444
BseDI CCNNGG 7 cut(s) 96, 305, 897, 1389, 1422, 2315, 2790
BseGI GGATG 6 cut(s) 300, 687, 1041, 1318, 1414, 1615
BseJI GATNNNNATC 3 cut(s) 498, 1116, 2586
BseLI CCNNNNNNNGG 9 cut(s) 306, 515, 1438, 2079, 2321, 2408, 2655, 2763, 2764
BseMI GCAATG 3 cut(s) 1247, 1405, 2698
BseMII CTCAG 4 cut(s) 213, 1473, 1792, 2652
BseNI ACTGG 7 cut(s) 53, 1225, 1623, 1844, 2023, 2187, 2402
BseRI GAGGAG 8 cut(s) 130, 133, 187, 623, 1411, 2451, 2484, 2526
BseSI GKGCMC 1 cut(s) 2844
BseX3I CGGCCG 2 cut(s) 237, 588
BseXI GCAGC 6 cut(s) 210, 1137, 1398, 1855, 1882, 2227
Bsh1236I CGCG 2 cut(s) 302, 665
Bsh1285I CGRYCG 4 cut(s) 82, 240, 591, 663
BshFI GGCC 8 cut(s) 57, 239, 299, 356, 590, 1890, 1968, 2320
BshNI GGYRCC 5 cut(s) 481, 667, 994, 2324, 2839
BshTI ACCGGT 1 cut(s) 2015
BshVI ATCGAT 1 cut(s) 1444
BsiEI CGRYCG 4 cut(s) 82, 240, 591, 663
BsiHKAI GWGCWC 2 cut(s) 1592, 2258
BsiHKCI CYCGRG 3 cut(s) 96, 453, 2229
BslFI GGGAC 4 cut(s) 65, 79, 1414, 1826
BslI CCNNNNNNNGG 9 cut(s) 306, 515, 1438, 2079, 2321, 2408, 2655, 2763, 2764
BsmAI GTCTC 3 cut(s) 199, 631, 2433
BsmBI CGTCTC 1 cut(s) 199
BsmFI GGGAC 4 cut(s) 65, 79, 1414, 1826
BsmI GAATGC 1 cut(s) 2362
BsnI GGCC 8 cut(s) 57, 239, 299, 356, 590, 1890, 1968, 2320
BsoBI CYCGRG 3 cut(s) 96, 453, 2229
Bsp119I TTCGAA 3 cut(s) 398, 524, 1596
Bsp1286I GDGCHC 4 cut(s) 1286, 1592, 2258, 2844
Bsp13I TCCGGA 2 cut(s) 502, 1438
Bsp1407I TGTACA 2 cut(s) 1932, 2395
Bsp19I CCATGG 2 cut(s) 1389, 2315
BspANI GGCC 8 cut(s) 57, 239, 299, 356, 590, 1890, 1968, 2320
BspCNI CTCAG 4 cut(s) 212, 1474, 1791, 2651
BspDI ATCGAT 1 cut(s) 1444
BspEI TCCGGA 2 cut(s) 502, 1438
BspFNI CGCG 2 cut(s) 302, 665
BspHI TCATGA 3 cut(s) 1276, 1852, 2275
BspMAI CTGCAG 1 cut(s) 2245
BspPI GGATC 7 cut(s) 488, 494, 1125, 1211, 1449, 2582, 2595
BspQI GCTCTTC 2 cut(s) 1516, 2217
BspT104I TTCGAA 3 cut(s) 398, 524, 1596
BspT107I GGYRCC 5 cut(s) 481, 667, 994, 2324, 2839
BsrDI GCAATG 3 cut(s) 1247, 1405, 2698
BsrFI RCCGGY 2 cut(s) 931, 2015
BsrGI TGTACA 2 cut(s) 1932, 2395
BsrI ACTGG 7 cut(s) 53, 1225, 1623, 1844, 2023, 2187, 2402
BssAI RCCGGY 2 cut(s) 931, 2015
BssECI CCNNGG 7 cut(s) 96, 305, 897, 1389, 1422, 2315, 2790
BssNI GRCGYC 6 cut(s) 213, 388, 417, 482, 668, 698
BssT1I CCWWGG 2 cut(s) 1389, 2315
Bst2UI CCWGG 4 cut(s) 1262, 1892, 2322, 2792
Bst6I CTCTTC 5 cut(s) 23, 1516, 1822, 2217, 2469
BstACI GRCGYC 6 cut(s) 213, 388, 417, 482, 668, 698
BstAUI TGTACA 2 cut(s) 1932, 2395
BstBI TTCGAA 3 cut(s) 398, 524, 1596
BstC8I GCNNGC 4 cut(s) 228, 329, 1873, 1910
BstDEI CTNAG 7 cut(s) 199, 1457, 1482, 1778, 2370, 2528, 2638
BstDSI CCRYGG 3 cut(s) 897, 1389, 2315
BstEII GGTNACC 3 cut(s) 1693, 1861, 2336
BstENI CCTNNNNNAGG 1 cut(s) 2653
BstF5I GGATG 6 cut(s) 300, 687, 1041, 1318, 1414, 1615
BstFNI CGCG 2 cut(s) 302, 665
BstH2I RGCGCY 3 cut(s) 485, 671, 750
BstHHI GCGC 4 cut(s) 304, 484, 670, 749
BstMAI GTCTC 3 cut(s) 199, 631, 2433
BstMCI CGRYCG 4 cut(s) 82, 240, 591, 663
BstNI CCWGG 4 cut(s) 1262, 1892, 2322, 2792
BstNSI RCATGY 1 cut(s) 1875
BstPAI GACNNNNGTC 1 cut(s) 168
BstPI GGTNACC 3 cut(s) 1693, 1861, 2336
BstSFI CTRYAG 1 cut(s) 2241
BstSLI GKGCMC 1 cut(s) 2844
BstUI CGCG 2 cut(s) 302, 665
BstV1I GCAGC 6 cut(s) 210, 1137, 1398, 1855, 1882, 2227
BstV2I GAAGAC 2 cut(s) 78, 2703
BstX2I RGATCY 1 cut(s) 2587
BstYI RGATCY 1 cut(s) 2587
BstZI CGGCCG 2 cut(s) 237, 588
Bsu15I ATCGAT 1 cut(s) 1444
BsuI GTATCC 1 cut(s) 2820
BsuRI GGCC 8 cut(s) 57, 239, 299, 356, 590, 1890, 1968, 2320
BsuTUI ATCGAT 1 cut(s) 1444
BtgI CCRYGG 3 cut(s) 897, 1389, 2315
BtsCI GGATG 6 cut(s) 300, 687, 1041, 1318, 1414, 1615
BtsIMutI CAGTG 4 cut(s) 621, 1774, 2194, 2294
Cac8I GCNNGC 4 cut(s) 228, 329, 1873, 1910
CaiI CAGNNNCTG 1 cut(s) 2414
CciI TCATGA 3 cut(s) 1276, 1852, 2275
CfoI GCGC 4 cut(s) 304, 484, 670, 749
Cfr10I RCCGGY 2 cut(s) 931, 2015
Cfr13I GGNCC 6 cut(s) 555, 1159, 1621, 1632, 2721, 2861
Cfr9I CCCGGG 1 cut(s) 96
ClaI ATCGAT 1 cut(s) 1444
CseI GACGC 5 cut(s) 181, 396, 406, 706, 2139
CspAI ACCGGT 1 cut(s) 2015
DdeI CTNAG 7 cut(s) 199, 1457, 1482, 1778, 2370, 2528, 2638
DinI GGCGCC 2 cut(s) 483, 669
DraI TTTAAA 1 cut(s) 1710
DrdI GACNNNNNNGTC 2 cut(s) 649, 1225
DseDI GACNNNNNNGTC 2 cut(s) 649, 1225
EaeI YGGCCR 5 cut(s) 237, 297, 354, 588, 1888
EagI CGGCCG 2 cut(s) 237, 588
Eam1104I CTCTTC 5 cut(s) 23, 1516, 1822, 2217, 2469
EarI CTCTTC 5 cut(s) 23, 1516, 1822, 2217, 2469
EciI GGCGGA 1 cut(s) 103
EclXI CGGCCG 2 cut(s) 237, 588
Eco130I CCWWGG 2 cut(s) 1389, 2315
Eco24I GRGCYC 1 cut(s) 1286
Eco47I GGWCC 6 cut(s) 555, 1159, 1621, 1632, 2721, 2861
Eco52I CGGCCG 2 cut(s) 237, 588
Eco57I CTGAAG 2 cut(s) 367, 1485
Eco88I CYCGRG 3 cut(s) 96, 453, 2229
Eco91I GGTNACC 3 cut(s) 1693, 1861, 2336
EcoNI CCTNNNNNAGG 1 cut(s) 2653
EcoO109I RGGNCCY 1 cut(s) 2861
EcoO65I GGTNACC 3 cut(s) 1693, 1861, 2336
EcoRI GAATTC 2 cut(s) 526, 1556
EcoRII CCWGG 4 cut(s) 1260, 1890, 2320, 2790
EcoT14I CCWWGG 2 cut(s) 1389, 2315
EcoT22I ATGCAT 1 cut(s) 2698
EcoT38I GRGCYC 1 cut(s) 1286
EgeI GGCGCC 2 cut(s) 483, 669
EheI GGCGCC 2 cut(s) 483, 669
ErhI CCWWGG 2 cut(s) 1389, 2315
Esp3I CGTCTC 1 cut(s) 199
FalI AAGNNNNNCTT 2 cut(s) 1292, 1324
FaqI GGGAC 4 cut(s) 65, 79, 1414, 1826
FauI CCCGC 1 cut(s) 2355
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 758
FokI GGATG 6 cut(s) 307, 694, 1048, 1325, 1401, 1622
FriOI GRGCYC 1 cut(s) 1286
FspBI CTAG 8 cut(s) 672, 678, 735, 1317, 1329, 1515, 2487, 2869
GlaI GCGC 4 cut(s) 303, 483, 669, 748
GsuI CTGGAG 2 cut(s) 1356, 1866
HaeII RGCGCY 3 cut(s) 485, 671, 750
HaeIII GGCC 8 cut(s) 57, 239, 299, 356, 590, 1890, 1968, 2320
HgaI GACGC 5 cut(s) 181, 396, 406, 706, 2139
HhaI GCGC 4 cut(s) 304, 484, 670, 749
Hin1I GRCGYC 6 cut(s) 213, 388, 417, 482, 668, 698
Hin6I GCGC 4 cut(s) 302, 482, 668, 747
HinP1I GCGC 4 cut(s) 302, 482, 668, 747
HincII GTYRAC 2 cut(s) 759, 967
HindII GTYRAC 2 cut(s) 759, 967
HindIII AAGCTT 4 cut(s) 579, 1467, 1799, 2760
Hpy166II GTNNAC 8 cut(s) 759, 768, 832, 901, 967, 1100, 1837, 2089
Hpy8I GTNNAC 8 cut(s) 759, 768, 832, 901, 967, 1100, 1837, 2089
HpyCH4IV ACGT 4 cut(s) 35, 213, 650, 2386
HpyF3I CTNAG 7 cut(s) 199, 1457, 1482, 1778, 2370, 2528, 2638
HpySE526I ACGT 4 cut(s) 35, 213, 650, 2386
Hsp92I GRCGYC 6 cut(s) 213, 388, 417, 482, 668, 698
HspAI GCGC 4 cut(s) 302, 482, 668, 747
KasI GGCGCC 2 cut(s) 481, 667
Kpn2I TCCGGA 2 cut(s) 502, 1438
KpnI GGTACC 1 cut(s) 998
Ksp22I TGATCA 1 cut(s) 3
LguI GCTCTTC 2 cut(s) 1516, 2217
LmnI GCTCC 6 cut(s) 10, 505, 1004, 1237, 1790, 2147
Lsp1109I GCAGC 6 cut(s) 210, 1137, 1398, 1855, 1882, 2227
LweI GCATC 9 cut(s) 453, 534, 939, 1303, 1423, 1600, 1783, 2426, 2705
MaeI CTAG 8 cut(s) 672, 678, 735, 1317, 1329, 1515, 2487, 2869
MaeII ACGT 4 cut(s) 35, 213, 650, 2386
MaeIII GTNAC 9 cut(s) 273, 430, 1693, 1735, 1861, 2056, 2101, 2336, 2779
MflI RGATCY 1 cut(s) 2587
MhlI GDGCHC 4 cut(s) 1286, 1592, 2258, 2844
MlsI TGGCCA 2 cut(s) 356, 1890
MluNI TGGCCA 2 cut(s) 356, 1890
Mly113I GGCGCC 2 cut(s) 482, 668
MlyI GAGTC 3 cut(s) 101, 521, 2636
MmeI TCCRAC 2 cut(s) 171, 836
Mox20I TGGCCA 2 cut(s) 356, 1890
Mph1103I ATGCAT 1 cut(s) 2698
MroI TCCGGA 2 cut(s) 502, 1438
MroXI GAANNNNTTC 2 cut(s) 2283, 2611
MscI TGGCCA 2 cut(s) 356, 1890
MseI TTAA 5 cut(s) 798, 1709, 1955, 1980, 2037
MslI CAYNNNNRTG 3 cut(s) 293, 1608, 2205
Msp20I TGGCCA 2 cut(s) 356, 1890
MspA1I CMGCKG 6 cut(s) 1150, 1411, 1782, 1868, 2240, 2552
Mva1269I GAATGC 1 cut(s) 2362
MvaI CCWGG 4 cut(s) 1262, 1892, 2322, 2792
MvnI CGCG 2 cut(s) 302, 665
NarI GGCGCC 2 cut(s) 482, 668
NciI CCSGG 6 cut(s) 97, 98, 306, 979, 2074, 2844
NcoI CCATGG 2 cut(s) 1389, 2315
NmeAIII GCCGAG 2 cut(s) 268, 566
NmuCI GTSAC 7 cut(s) 273, 1693, 1861, 2056, 2101, 2336, 2779
NsiI ATGCAT 1 cut(s) 2698
NspI RCATGY 1 cut(s) 1875
NspV TTCGAA 3 cut(s) 398, 524, 1596
PaeI GCATGC 1 cut(s) 1875
PaeR7I CTCGAG 1 cut(s) 2229
PagI TCATGA 3 cut(s) 1276, 1852, 2275
PciSI GCTCTTC 2 cut(s) 1516, 2217
PcsI WCGNNNNNNNCGW 8 cut(s) 60, 72, 189, 266, 521, 530, 536, 656
PctI GAATGC 1 cut(s) 2362
PdmI GAANNNNTTC 2 cut(s) 2283, 2611
PfeI GAWTC 9 cut(s) 1267, 1405, 1996, 2069, 2195, 2451, 2612, 2669, 2741
PflFI GACNNNGTC 3 cut(s) 374, 1346, 1541
PflMI CCANNNNNTGG 1 cut(s) 2321
PfoI TCCNGGA 1 cut(s) 2072
PinAI ACCGGT 1 cut(s) 2015
Ple19I CGATCG 1 cut(s) 663
PleI GAGTC 3 cut(s) 100, 520, 2635
PluTI GGCGCC 2 cut(s) 485, 671
PpsI GAGTC 3 cut(s) 100, 520, 2635
PpuMI RGGWCCY 1 cut(s) 2861
PshAI GACNNNNGTC 1 cut(s) 168
PshBI ATTAAT 1 cut(s) 2037
Psp1406I AACGTT 1 cut(s) 2386
Psp5II RGGWCCY 1 cut(s) 2861
Psp6I CCWGG 4 cut(s) 1260, 1890, 2320, 2790
PspEI GGTNACC 3 cut(s) 1693, 1861, 2336
PspGI CCWGG 4 cut(s) 1260, 1890, 2320, 2790
PspPI GGNCC 6 cut(s) 555, 1159, 1621, 1632, 2721, 2861
PspPPI RGGWCCY 1 cut(s) 2861
PspXI VCTCGAGB 1 cut(s) 2229
PsrI GAACNNNNNNTAC 2 cut(s) 1350, 1382
PstI CTGCAG 1 cut(s) 2245
PstNI CAGNNNCTG 1 cut(s) 2414
PsuI RGATCY 1 cut(s) 2587
PsyI GACNNNGTC 3 cut(s) 374, 1346, 1541
PvuI CGATCG 1 cut(s) 663
PvuII CAGCTG 1 cut(s) 2240
RgaI GCGATCGC 1 cut(s) 663
RseI CAYNNNNRTG 3 cut(s) 293, 1608, 2205
SalI GTCGAC 1 cut(s) 757
SapI GCTCTTC 2 cut(s) 1516, 2217
SaqAI TTAA 5 cut(s) 798, 1709, 1955, 1980, 2037
Sau96I GGNCC 6 cut(s) 555, 1159, 1621, 1632, 2721, 2861
ScaI AGTACT 3 cut(s) 1617, 2491, 2533
SchI GAGTC 3 cut(s) 101, 521, 2636
SduI GDGCHC 4 cut(s) 1286, 1592, 2258, 2844
SfaAI GCGATCGC 1 cut(s) 663
SfaNI GCATC 9 cut(s) 453, 534, 939, 1303, 1423, 1600, 1783, 2426, 2705
SfcI CTRYAG 1 cut(s) 2241
SfoI GGCGCC 2 cut(s) 483, 669
Sfr274I CTCGAG 1 cut(s) 2229
SfuI TTCGAA 3 cut(s) 398, 524, 1596
SgfI GCGATCGC 1 cut(s) 663
SgrAI CRCCGGYG 1 cut(s) 931
SinI GGWCC 6 cut(s) 555, 1159, 1621, 1632, 2721, 2861
SlaI CTCGAG 1 cut(s) 2229
SmaI CCCGGG 1 cut(s) 98
SmiMI CAYNNNNRTG 3 cut(s) 293, 1608, 2205
SmlI CTYRAG 2 cut(s) 2229, 2427
SmoI CTYRAG 2 cut(s) 2229, 2427
SphI GCATGC 1 cut(s) 1875
SspDI GGCGCC 2 cut(s) 481, 667
SspI AATATT 1 cut(s) 2282
SspMI CTAG 8 cut(s) 672, 678, 735, 1317, 1329, 1515, 2487, 2869
StyI CCWWGG 2 cut(s) 1389, 2315
TaiI ACGT 4 cut(s) 38, 216, 653, 2389
TatI WGTACW 7 cut(s) 1099, 1615, 1932, 2172, 2395, 2489, 2531
TfiI GAWTC 9 cut(s) 1267, 1405, 1996, 2069, 2195, 2451, 2612, 2669, 2741
Tru1I TTAA 5 cut(s) 798, 1709, 1955, 1980, 2037
Tru9I TTAA 5 cut(s) 798, 1709, 1955, 1980, 2037
TscAI CASTG 4 cut(s) 628, 1774, 2194, 2294
TseFI GTSAC 7 cut(s) 273, 1693, 1861, 2056, 2101, 2336, 2779
TseI GCWGC 6 cut(s) 223, 1150, 1411, 1868, 1895, 2240
Tsp45I GTSAC 7 cut(s) 273, 1693, 1861, 2056, 2101, 2336, 2779
TspDTI ATGAA 8 cut(s) 59, 814, 1343, 1644, 1841, 1869, 2292, 2754
TspGWI ACGGA 4 cut(s) 776, 907, 2448, 2624
TspMI CCCGGG 1 cut(s) 96
TspRI CASTG 4 cut(s) 628, 1774, 2194, 2294
Tth111I GACNNNGTC 3 cut(s) 374, 1346, 1541
Van91I CCANNNNNTGG 1 cut(s) 2321
VpaK11BI GGWCC 6 cut(s) 555, 1159, 1621, 1632, 2721, 2861
VspI ATTAAT 1 cut(s) 2037
XagI CCTNNNNNAGG 1 cut(s) 2653
XapI RAATTY 4 cut(s) 526, 1556, 2598, 2818
XceI RCATGY 1 cut(s) 1875
XhoI CTCGAG 1 cut(s) 2229
XmaI CCCGGG 1 cut(s) 96
XmiI GTMKAC 1 cut(s) 758
XmnI GAANNNNTTC 2 cut(s) 2283, 2611
XspI CTAG 8 cut(s) 672, 678, 735, 1317, 1329, 1515, 2487, 2869
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 3 cut(s) 1617, 2491, 2533
Zsp2I ATGCAT 1 cut(s) 2698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.