FvH4_6g13960

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012301
Physical Location & Seq
Forward (+)
8557523 .. 8561644
4122 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g13960.t1

Sequence Viewer

Length: 2814 bp
ATGATCATGGACAGGAAAATGGAGCGGAAGAGGACCTTCACTATGAACTGGGACGGCCTCCACGACCAAGAAGACGACTCCTTCTTCGAATCCCGAGAGCGGATGTCCTCCGCCGTCGCTATCGACCTCGAAGCCTCCTCCTCCGACGATGAGGACTTCGACGACAGCCGTATGTCCTTCGCCTCCTGCGCCTCCTCCCTCCGAAACGACCTCGAGGTCGAGGTCCGAGCCTTGTCCGGCGCCGCCGCCGAGCCTTCGTTTTCCTCTCCTCCGTTGACGCCGGACTACAACATCTGGATGGCCGCGCCGGGGTCGATCTCCGAGCGACGCAGGCGGTTGATGCAGGGGATGGGATTGGCCAACGGCGGCAGAGACATCGTCAGCTTTAAACGCATCGTTTCGAGTAAACCTCCAAACGGCGTCATTCCGGCCGTTACGAAATCCCCTACGCTTTCTGTGGAAAAGATACAAGTTCCTGAACCGGCGCCGCATGATCCGGAGGCGGAGGCGGAGGCGTCGAATTCGCCGTTGCAGGCGGTGCTTGTACGGTCGAGATCGGACGGAGAAATCGAGGCTTTCTCAGCGGATAAACGGAGGAAAGAGGAAGTGATCGGCGCAATCTCGAAGCAACGGCTGACTCGGACGTCGTCGACGATCGCGGCGCCGAGCACGAGAATGTTTTCGTACCCGGAGGTTGTGGCGACGACGCCGCAAAGCGACGGAGGAGCCAGGGCCGTCCGATCTAGACAGAACAGCACGGCGTTGTCGACGGAGTTCACTAACAATTTAGGAGCTTTCTTCTTGATTAAGAATCTGGACACCGGAACCGAGTTCATTGTGAACGAGTACGATCAGAACGGGTGTTGGAACAAGTTCAGTGATCTTCAAACGGGGAAGAAATTGACCATGGACGAGTTCGAGAAGTCCGTGGGGTACTCGCCGGTGGTGAAGGAGGTGATGCGGCGGCAGAATGTGACCAGCGACGCCGTGAATGAGAGGAAAGTTGGTTCACCCGGAGCCAATTCATTTGTGTCGAAAAGTTTGAGGATGAGCAAGCGAAAGGTGGCGCTGTTGAAGAATAGCATAAAAGGTGTGGGGAGTGCTGTGAGTGTGCTAATTGGGGATAAGGATCAACACCCGCCACCGCCGCTGCCGCAGGACCAGAAACCGAGCAAGTCTAATGAGTGGATCAAAGTTAGGCAGAATGGGAAGTCGTATAAGGAGCTATCAGCATTGCATTTGTGCCAGGAGATTCAAGCTCATGAGGGCTCAATTTGGACTATTAAGTTTAGCTTGGATGCTAGGTTTCTTGCTAGTGCTGGAGAGGATAGGGTCATTCATGTGTGGGAGGTTCAGGAATGTGAGATTATGTCCATGGATAGCAATGCGACCCCACTGCATCCCTCGTTGTCTCAGGATCGATCGGGGCCTCTTAGCTCTGAAGCGGCAATTTCGTCTGAGAAGAAGAAGAAGGGGAAAGGAGGCTCTTCCAGGAAAACAAATCCCATTCCTGACTATGTCCATGTGCCTGAATTCGTGTTTGCCCTCTCGGAGAAACCTGTGTGCTCTTTCGAAGGTCATTTGGATGCTGTACTGGACCTGTCATGGTCCAGAGATCAGCTGTTGCTTTCATCTTCAATGGACAAAACTGTTAGATTATGGGATATGGAGACCAAGAGTTGTTTGAAGATGTTTGCCCACAATGATTATGTAACTTGCATACAGTTCAATCCTTTGGACGACAATGTTTTTCTCAGCGGCTCCCTTGATGCAAAGCTTCGATTATGGGACATACCTGCTCGAAAAGTGGTAGACTGGACAGATCTTCATGAAATGGTCACTGCTGCATGCTATACTCCAGATGGCCAGGCTGCCATCACTGGCTCACATAAAGGGAACTGCCGTATGTACAATGCAGAAGACTGTAAATTAAGTCAAGAAGGACAGATTGATATCAAAAACAAGAAGAAGAGTCAAGCAAAGAAAATTACTGGTTTCCAGTTTTCTCCAACCAATCCATCCGAAATGCTTGTCACATCTGCTGATTCCCGGATTCGGATTGTAGATGGTGATAAAGATGTCACTCATAAGTTTAGAGGTTTTCGAAACACAAGTAGTCAAATTGGAGCGTCATTCACTCCCAATGGAAAGTACATTATTTGTGCTAGTGAAGACTCTAATGTATATGTATGGAAGCGTGAAGAGCCTCGAGTGTCAGCCGCAGGAAAAAAGAGCACAATCACCACCACTTCTCATGAATATTTTCAGTGTAAAGATGTATCAGTAGCAATCCCATGGCCTGGCACCATAAAGGGTGACCCTCCCCCATCTATACCTGTGCATTCTTCTAAGAGACACTCAAAACGTTCCTCTGTACAGCCAGTTTCAGGAACTGCATCGCCTACTAAAGAAGAGAGCAAAAGACTGTTGCCACCTCTCCCAAAGAAACACACTAATCCTAGCACTCCTCCTACGGCTGCGGCAGCGGAGAGACCTTCAACTCCCCCTACGGAAGATCAAGATCCATCTGAAATATCTCGAACGGATTCTGGCATTGGCGAGTCATTCGGCAGCAGCTCAGACCCTTCCTCTATTAGGTACAGTGGCGACTCATCTTCTATATTTTCTGGCAATGCATCATCATCATCTTGGTCTTCCTCTTGGTCCTCTTTGTTTGGGCATGAATCGAGCCAAACAACGCAAGCTTGGGGCTTGGTCATTGTCACAGCCACTCTGGAAGGTGAAATCAGAGCATATCAAAATTTTGGTATACCAAGAAGGGTGCAGAGCAATATTTTTGGAGGTCCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

938

Amino Acids

102.89

Weight (kDa)

7.27

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 408 - 640 3.9e-19 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 410 - 456 7.8e-07 THOC3 beta-propeller domain
WD40 PF00400 415 - 449 7.5e-06 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 518 - 581 1.4e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 518 - 638 2.5e-17 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 518 - 748 7.7e-21 Prp19 WD40 domain
WD40 PF00400 519 - 555 6.8e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 519 - 740 2.6e-40 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 520 - 637 4.4e-12 CDC20/Fizzy WD40 domain
EIF3I PF24805 521 - 721 1.1e-10 EIF3I
WDR55 PF24796 521 - 644 1.1e-08 WDR55
Beta-prop_THOC3 PF25174 523 - 564 3.5e-07 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 524 - 582 5.9e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_IFT122_1st PF23381 524 - 638 5.7e-07 IFT122 first beta-propeller
Beta-prop_THOC3 PF25174 527 - 735 2.4e-27 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 529 - 644 2.9e-10 Aladin seven-bladed propeller
WD40_Gbeta PF25391 538 - 731 3.7e-12 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 542 - 642 1.3e-08 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 570 - 737 2.4e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 581 - 737 5.8e-15 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37670 AT2G37670 AT5G02430
fragaria_vesca FvH4_6g13960
malus_domestica MD04G1127300.v1.1 MD12G1140500.v1.1
prunus_persica Prupe.6G253900_v2.0.a1 Prupe.6G253900_v2.0.a1
pyrus_communis pycom04g11410
rosa_chinensis RchiOBHm_Chr3g0466781
rosa_laevigata RLG00000024553
rosa_multiflora Rmu_sc0008017.1_g000009
rosa_roxburghii Rroxscaffold_6G00414170
rosa_rugosa Rorug03G0083800
rosa_samantha Rh3AG131000 Rh3BG152000 Rh3CG151900 Rh3DG152700
rosa_wichuraiana Rw3G012380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 215, 643
AatII GACGTC 1 cut(s) 647
AbsI CCTCGAGG 1 cut(s) 212
Acc36I ACCTGC 1 cut(s) 1804
AccB1I GGYRCC 4 cut(s) 239, 484, 661, 2303
AccB7I CCANNNNNTGG 1 cut(s) 2300
AccBSI CCGCTC 2 cut(s) 25, 100
AccI GTMKAC 4 cut(s) 650, 767, 1812, 2770
AccII CGCG 2 cut(s) 305, 659
AccIII TCCGGA 1 cut(s) 496
AclI AACGTT 1 cut(s) 2365
AclWI GGATC 5 cut(s) 488, 1137, 1196, 1425, 2516
AcoI YGGCCR 4 cut(s) 300, 357, 429, 1864
AcsI RAATTY 3 cut(s) 520, 1532, 2761
AcuI CTGAAG 1 cut(s) 1461
AcyI GRCGYC 9 cut(s) 240, 278, 420, 485, 515, 644, 662, 707, 984
AfaI GTAC 9 cut(s) 546, 686, 848, 935, 1593, 1910, 2153, 2376, 2600
AfiI CCNNNNNNNGG 7 cut(s) 99, 309, 416, 2055, 2300, 2387, 2595
AgsI TTSAA 7 cut(s) 887, 1075, 1256, 1638, 1687, 1729, 2499
AjnI CCWGG 5 cut(s) 728, 1245, 1490, 1866, 2299
AloI GAACNNNNNNTCC 2 cut(s) 991, 1023
Alw21I GWGCWC 3 cut(s) 671, 1568, 2237
Alw26I GTCTC 5 cut(s) 366, 1416, 1664, 2347, 2485
AlwI GGATC 5 cut(s) 488, 1137, 1196, 1425, 2516
AlwNI CAGNNNCTG 1 cut(s) 2393
Ama87I CYCGRG 3 cut(s) 93, 212, 2208
Aor13HI TCCGGA 1 cut(s) 496
AoxI GGCC 8 cut(s) 55, 300, 357, 429, 732, 1427, 1864, 2297
ApeKI GCWGC 7 cut(s) 1150, 1844, 1871, 2477, 2483, 2571, 2574
ApoI RAATTY 3 cut(s) 520, 1532, 2761
ArsI GACNNNNNNTTYG 4 cut(s) 68, 100, 2016, 2048
Asp700I GAANNNNTTC 4 cut(s) 679, 872, 2262, 2545
AspLEI GCGC 7 cut(s) 191, 242, 307, 487, 617, 664, 1069
AspS9I GGNCC 9 cut(s) 33, 223, 732, 1159, 1427, 1597, 1608, 2664, 2804
AsuC2I CCSGG 4 cut(s) 309, 689, 1014, 2050
AsuHPI GGTGA 7 cut(s) 958, 967, 1002, 2081, 2233, 2327, 2753
AsuII TTCGAA 3 cut(s) 87, 1572, 2104
AvaI CYCGRG 3 cut(s) 93, 212, 2208
AvaII GGWCC 7 cut(s) 33, 223, 1159, 1597, 1608, 2664, 2804
BalI TGGCCA 2 cut(s) 359, 1866
BanI GGYRCC 4 cut(s) 239, 484, 661, 2303
BanII GRGCYC 1 cut(s) 1271
BauI CACGAG 1 cut(s) 670
BbsI GAAGAC 4 cut(s) 78, 1926, 2178, 2646
Bbv12I GWGCWC 3 cut(s) 671, 1568, 2237
BbvI GCAGC 7 cut(s) 1137, 1831, 1858, 2464, 2495, 2583, 2586
BccI CCATC 8 cut(s) 292, 343, 1856, 1883, 2026, 2060, 2335, 2533
BcgI CGANNNNNNTGC 4 cut(s) 358, 392, 1378, 1412
BciT130I CCWGG 5 cut(s) 730, 1247, 1492, 1868, 2301
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 4 cut(s) 309, 689, 1014, 2050
BcoDI GTCTC 5 cut(s) 366, 1416, 1664, 2347, 2485
BfaI CTAG 6 cut(s) 744, 1302, 1314, 2166, 2460, 2812
BfoI RGCGCY 4 cut(s) 243, 488, 665, 1070
BfuAI ACCTGC 1 cut(s) 1804
BglII AGATCT 1 cut(s) 1822
Bme1390I CCNGG 9 cut(s) 309, 689, 730, 1014, 1247, 1492, 1868, 2050, 2301
Bme18I GGWCC 7 cut(s) 33, 223, 1159, 1597, 1608, 2664, 2804
BmeT110I CYCGRG 3 cut(s) 93, 212, 2208
BmgT120I GGNCC 9 cut(s) 33, 223, 732, 1159, 1427, 1597, 1608, 2664, 2804
BmiI GGNNCC 9 cut(s) 241, 486, 663, 727, 826, 1018, 1428, 1762, 2305
BmrFI CCNGG 9 cut(s) 309, 689, 730, 1014, 1247, 1492, 1868, 2050, 2301
BmrI ACTGGG 1 cut(s) 58
BmsI GCATC 9 cut(s) 330, 402, 948, 1288, 1408, 1576, 1759, 2405, 2645
BmuI ACTGGG 1 cut(s) 58
BpiI GAAGAC 4 cut(s) 78, 1926, 2178, 2646
BplI GAGNNNNNCTC 4 cut(s) 394, 426, 563, 595
BpmI CTGGAG 2 cut(s) 1341, 1842
Bpu14I TTCGAA 3 cut(s) 87, 1572, 2104
BpuMI CCSGG 4 cut(s) 309, 689, 1014, 2050
Bsa29I ATCGAT 1 cut(s) 1420
BsaBI GATNNNNATC 3 cut(s) 1128, 1952, 2520
BsaHI GRCGYC 9 cut(s) 240, 278, 420, 485, 515, 644, 662, 707, 984
BsaI GGTCTC 2 cut(s) 1664, 2485
BsaJI CCNNGG 6 cut(s) 308, 729, 906, 927, 1374, 2294
BsaWI WCCGGW 2 cut(s) 496, 821
Bsc4I CCNNNNNNNGG 7 cut(s) 99, 309, 416, 2055, 2300, 2387, 2595
Bse118I RCCGGY 2 cut(s) 481, 940
Bse1I ACTGG 7 cut(s) 53, 1599, 1820, 1885, 1996, 1999, 2381
Bse3DI GCAATG 3 cut(s) 1232, 1390, 2638
Bse8I GATNNNNATC 3 cut(s) 1128, 1952, 2520
BseAI TCCGGA 1 cut(s) 496
BseBI CCWGG 5 cut(s) 730, 1247, 1492, 1868, 2301
BseCI ATCGAT 1 cut(s) 1420
BseDI CCNNGG 6 cut(s) 308, 729, 906, 927, 1374, 2294
BseGI GGATG 8 cut(s) 108, 303, 354, 1053, 1303, 1399, 1591, 2018
BseJI GATNNNNATC 3 cut(s) 1128, 1952, 2520
BseLI CCNNNNNNNGG 7 cut(s) 99, 309, 416, 2055, 2300, 2387, 2595
BseMI GCAATG 3 cut(s) 1232, 1390, 2638
BseMII CTCAG 5 cut(s) 594, 1427, 1449, 1768, 2592
BseNI ACTGG 7 cut(s) 53, 1599, 1820, 1885, 1996, 1999, 2381
BseRI GAGGAG 6 cut(s) 127, 130, 184, 258, 738, 2457
BseX3I CGGCCG 1 cut(s) 429
BseXI GCAGC 7 cut(s) 1137, 1831, 1858, 2464, 2495, 2583, 2586
BsgI GTGCAG 1 cut(s) 2804
Bsh1236I CGCG 2 cut(s) 305, 659
Bsh1285I CGRYCG 4 cut(s) 432, 551, 657, 1424
BshFI GGCC 8 cut(s) 57, 302, 359, 431, 734, 1429, 1866, 2299
BshNI GGYRCC 4 cut(s) 239, 484, 661, 2303
BshVI ATCGAT 1 cut(s) 1420
BsiEI CGRYCG 4 cut(s) 432, 551, 657, 1424
BsiHKAI GWGCWC 3 cut(s) 671, 1568, 2237
BsiHKCI CYCGRG 3 cut(s) 93, 212, 2208
BslFI GGGAC 2 cut(s) 65, 1802
BslI CCNNNNNNNGG 7 cut(s) 99, 309, 416, 2055, 2300, 2387, 2595
BsmAI GTCTC 5 cut(s) 366, 1416, 1664, 2347, 2485
BsmFI GGGAC 2 cut(s) 65, 1802
BsmI GAATGC 1 cut(s) 2341
BsnI GGCC 8 cut(s) 57, 302, 359, 431, 734, 1429, 1866, 2299
Bso31I GGTCTC 2 cut(s) 1664, 2485
BsoBI CYCGRG 3 cut(s) 93, 212, 2208
Bsp119I TTCGAA 3 cut(s) 87, 1572, 2104
Bsp1286I GDGCHC 4 cut(s) 671, 1271, 1568, 2237
Bsp13I TCCGGA 1 cut(s) 496
Bsp1407I TGTACA 2 cut(s) 1908, 2374
Bsp19I CCATGG 3 cut(s) 906, 1374, 2294
BspANI GGCC 8 cut(s) 57, 302, 359, 431, 734, 1429, 1866, 2299
BspCNI CTCAG 5 cut(s) 593, 1426, 1450, 1767, 2591
BspDI ATCGAT 1 cut(s) 1420
BspEI TCCGGA 1 cut(s) 496
BspFNI CGCG 2 cut(s) 305, 659
BspHI TCATGA 3 cut(s) 1261, 1828, 2254
BspLI GGNNCC 9 cut(s) 241, 486, 663, 727, 826, 1018, 1428, 1762, 2305
BspMI ACCTGC 1 cut(s) 1804
BspPI GGATC 5 cut(s) 488, 1137, 1196, 1425, 2516
BspQI GCTCTTC 2 cut(s) 1492, 2196
BspT104I TTCGAA 3 cut(s) 87, 1572, 2104
BspT107I GGYRCC 4 cut(s) 239, 484, 661, 2303
BspTNI GGTCTC 2 cut(s) 1664, 2485
BsrBI CCGCTC 2 cut(s) 25, 100
BsrDI GCAATG 3 cut(s) 1232, 1390, 2638
BsrFI RCCGGY 2 cut(s) 481, 940
BsrGI TGTACA 2 cut(s) 1908, 2374
BsrI ACTGG 7 cut(s) 53, 1599, 1820, 1885, 1996, 1999, 2381
BssAI RCCGGY 2 cut(s) 481, 940
BssECI CCNNGG 6 cut(s) 308, 729, 906, 927, 1374, 2294
BssNAI GTATAC 1 cut(s) 2771
BssNI GRCGYC 9 cut(s) 240, 278, 420, 485, 515, 644, 662, 707, 984
BssSI CACGAG 1 cut(s) 670
BssT1I CCWWGG 3 cut(s) 906, 1374, 2294
Bst1107I GTATAC 1 cut(s) 2771
Bst2BI CACGAG 1 cut(s) 670
Bst2UI CCWGG 5 cut(s) 730, 1247, 1492, 1868, 2301
Bst4CI ACNGT 6 cut(s) 549, 1651, 1725, 1925, 2427, 2603
Bst6I CTCTTC 5 cut(s) 23, 1492, 1964, 2196, 2406
BstACI GRCGYC 9 cut(s) 240, 278, 420, 485, 515, 644, 662, 707, 984
BstAPI GCANNNNNTGC 1 cut(s) 538
BstAUI TGTACA 2 cut(s) 1908, 2374
BstBI TTCGAA 3 cut(s) 87, 1572, 2104
BstC8I GCNNGC 5 cut(s) 332, 534, 1055, 1849, 2703
BstDEI CTNAG 7 cut(s) 580, 1413, 1433, 1458, 1754, 2349, 2578
BstDSI CCRYGG 4 cut(s) 906, 927, 1374, 2294
BstEII GGTNACC 1 cut(s) 2315
BstENI CCTNNNNNAGG 1 cut(s) 2593
BstF5I GGATG 8 cut(s) 108, 303, 354, 1053, 1303, 1399, 1591, 2018
BstFNI CGCG 2 cut(s) 305, 659
BstH2I RGCGCY 4 cut(s) 243, 488, 665, 1070
BstHHI GCGC 7 cut(s) 191, 242, 307, 487, 617, 664, 1069
BstMAI GTCTC 5 cut(s) 366, 1416, 1664, 2347, 2485
BstMCI CGRYCG 4 cut(s) 432, 551, 657, 1424
BstNI CCWGG 5 cut(s) 730, 1247, 1492, 1868, 2301
BstNSI RCATGY 1 cut(s) 1851
BstPI GGTNACC 1 cut(s) 2315
BstSCI CCNGG 9 cut(s) 307, 687, 728, 1012, 1245, 1490, 1866, 2048, 2299
BstUI CGCG 2 cut(s) 305, 659
BstV1I GCAGC 7 cut(s) 1137, 1831, 1858, 2464, 2495, 2583, 2586
BstV2I GAAGAC 4 cut(s) 78, 1926, 2178, 2646
BstX2I RGATCY 2 cut(s) 1822, 2521
BstYI RGATCY 2 cut(s) 1822, 2521
BstZ17I GTATAC 1 cut(s) 2771
BstZI CGGCCG 1 cut(s) 429
Bsu15I ATCGAT 1 cut(s) 1420
BsuRI GGCC 8 cut(s) 57, 302, 359, 431, 734, 1429, 1866, 2299
BsuTUI ATCGAT 1 cut(s) 1420
BtgI CCRYGG 4 cut(s) 906, 927, 1374, 2294
BtgZI GCGATG 1 cut(s) 2382
BtsCI GGATG 8 cut(s) 108, 303, 354, 1053, 1303, 1399, 1591, 2018
BtsI GCAGTG 2 cut(s) 1394, 1839
BtsIMutI CAGTG 6 cut(s) 883, 1394, 1839, 1878, 2273, 2608
BveI ACCTGC 1 cut(s) 1804
Cac8I GCNNGC 5 cut(s) 332, 534, 1055, 1849, 2703
CaiI CAGNNNCTG 1 cut(s) 2393
CciI TCATGA 3 cut(s) 1261, 1828, 2254
CfoI GCGC 7 cut(s) 191, 242, 307, 487, 617, 664, 1069
Cfr10I RCCGGY 2 cut(s) 481, 940
Cfr13I GGNCC 9 cut(s) 33, 223, 732, 1159, 1427, 1597, 1608, 2664, 2804
ClaI ATCGAT 1 cut(s) 1420
CseI GACGC 7 cut(s) 286, 336, 409, 504, 715, 992, 2118
Csp6I GTAC 9 cut(s) 545, 685, 847, 934, 1592, 1909, 2152, 2375, 2599
CviQI GTAC 9 cut(s) 545, 685, 847, 934, 1592, 1909, 2152, 2375, 2599
DdeI CTNAG 7 cut(s) 580, 1413, 1433, 1458, 1754, 2349, 2578
DinI GGCGCC 3 cut(s) 241, 486, 663
DraI TTTAAA 1 cut(s) 388
DrdI GACNNNNNNGTC 2 cut(s) 215, 643
DseDI GACNNNNNNGTC 2 cut(s) 215, 643
EaeI YGGCCR 4 cut(s) 300, 357, 429, 1864
EagI CGGCCG 1 cut(s) 429
Eam1104I CTCTTC 5 cut(s) 23, 1492, 1964, 2196, 2406
EarI CTCTTC 5 cut(s) 23, 1492, 1964, 2196, 2406
EciI GGCGGA 3 cut(s) 100, 518, 524
EclXI CGGCCG 1 cut(s) 429
Eco130I CCWWGG 3 cut(s) 906, 1374, 2294
Eco24I GRGCYC 1 cut(s) 1271
Eco31I GGTCTC 2 cut(s) 1664, 2485
Eco32I GATATC 1 cut(s) 1954
Eco47I GGWCC 7 cut(s) 33, 223, 1159, 1597, 1608, 2664, 2804
Eco52I CGGCCG 1 cut(s) 429
Eco57I CTGAAG 1 cut(s) 1461
Eco88I CYCGRG 3 cut(s) 93, 212, 2208
Eco91I GGTNACC 1 cut(s) 2315
EcoNI CCTNNNNNAGG 1 cut(s) 2593
EcoO109I RGGNCCY 3 cut(s) 33, 1427, 2804
EcoO65I GGTNACC 1 cut(s) 2315
EcoRI GAATTC 2 cut(s) 520, 1532
EcoRII CCWGG 5 cut(s) 728, 1245, 1490, 1866, 2299
EcoRV GATATC 1 cut(s) 1954
EcoT14I CCWWGG 3 cut(s) 906, 1374, 2294
EcoT22I ATGCAT 1 cut(s) 2638
EcoT38I GRGCYC 1 cut(s) 1271
EgeI GGCGCC 3 cut(s) 241, 486, 663
EheI GGCGCC 3 cut(s) 241, 486, 663
ErhI CCWWGG 3 cut(s) 906, 1374, 2294
FalI AAGNNNNNCTT 4 cut(s) 20, 52, 1277, 1309
FaqI GGGAC 2 cut(s) 65, 1802
FauI CCCGC 1 cut(s) 1146
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 4 cut(s) 650, 767, 1812, 2770
FokI GGATG 8 cut(s) 115, 310, 361, 1060, 1310, 1386, 1598, 2005
FriOI GRGCYC 1 cut(s) 1271
FspBI CTAG 6 cut(s) 744, 1302, 1314, 2166, 2460, 2812
GlaI GCGC 7 cut(s) 190, 241, 306, 486, 616, 663, 1068
GsuI CTGGAG 2 cut(s) 1341, 1842
HaeII RGCGCY 4 cut(s) 243, 488, 665, 1070
HaeIII GGCC 8 cut(s) 57, 302, 359, 431, 734, 1429, 1866, 2299
HgaI GACGC 7 cut(s) 286, 336, 409, 504, 715, 992, 2118
HhaI GCGC 7 cut(s) 191, 242, 307, 487, 617, 664, 1069
Hin1I GRCGYC 9 cut(s) 240, 278, 420, 485, 515, 644, 662, 707, 984
Hin6I GCGC 7 cut(s) 189, 240, 305, 485, 615, 662, 1067
HinP1I GCGC 7 cut(s) 189, 240, 305, 485, 615, 662, 1067
HincII GTYRAC 3 cut(s) 276, 651, 768
HindII GTYRAC 3 cut(s) 276, 651, 768
HindIII AAGCTT 2 cut(s) 1775, 2703
HphI GGTGA 7 cut(s) 958, 967, 1002, 2081, 2233, 2327, 2753
Hpy166II GTNNAC 9 cut(s) 276, 407, 651, 768, 777, 841, 1010, 1813, 2771
Hpy8I GTNNAC 9 cut(s) 276, 407, 651, 768, 777, 841, 1010, 1813, 2771
HpyCH4III ACNGT 6 cut(s) 549, 1651, 1725, 1925, 2427, 2603
HpyCH4IV ACGT 2 cut(s) 644, 2365
HpyF3I CTNAG 7 cut(s) 580, 1413, 1433, 1458, 1754, 2349, 2578
HpySE526I ACGT 2 cut(s) 644, 2365
Hsp92I GRCGYC 9 cut(s) 240, 278, 420, 485, 515, 644, 662, 707, 984
HspAI GCGC 7 cut(s) 189, 240, 305, 485, 615, 662, 1067
KasI GGCGCC 3 cut(s) 239, 484, 661
Kpn2I TCCGGA 1 cut(s) 496
Ksp22I TGATCA 1 cut(s) 3
LguI GCTCTTC 2 cut(s) 1492, 2196
LmnI GCTCC 7 cut(s) 22, 725, 791, 1016, 1222, 1766, 2126
Lsp1109I GCAGC 7 cut(s) 1137, 1831, 1858, 2464, 2495, 2583, 2586
LweI GCATC 9 cut(s) 330, 402, 948, 1288, 1408, 1576, 1759, 2405, 2645
MaeI CTAG 6 cut(s) 744, 1302, 1314, 2166, 2460, 2812
MaeII ACGT 2 cut(s) 644, 2365
MaeIII GTNAC 8 cut(s) 433, 973, 1711, 1837, 2032, 2080, 2315, 2722
MbiI CCGCTC 2 cut(s) 25, 100
MflI RGATCY 2 cut(s) 1822, 2521
MhlI GDGCHC 4 cut(s) 671, 1271, 1568, 2237
MlsI TGGCCA 2 cut(s) 359, 1866
MluNI TGGCCA 2 cut(s) 359, 1866
Mly113I GGCGCC 3 cut(s) 240, 485, 662
MlyI GAGTC 6 cut(s) 71, 631, 1981, 2168, 2570, 2603
MmeI TCCRAC 3 cut(s) 168, 845, 2033
Mox20I TGGCCA 2 cut(s) 359, 1866
Mph1103I ATGCAT 1 cut(s) 2638
MroI TCCGGA 1 cut(s) 496
MroXI GAANNNNTTC 4 cut(s) 679, 872, 2262, 2545
MscI TGGCCA 2 cut(s) 359, 1866
MseI TTAA 4 cut(s) 387, 807, 1284, 1931
MslI CAYNNNNRTG 4 cut(s) 296, 674, 1340, 1584
Msp20I TGGCCA 2 cut(s) 359, 1866
MspA1I CMGCKG 5 cut(s) 584, 1150, 1621, 1758, 2486
MspR9I CCNGG 9 cut(s) 309, 689, 730, 1014, 1247, 1492, 1868, 2050, 2301
Mva1269I GAATGC 1 cut(s) 2341
MvaI CCWGG 5 cut(s) 730, 1247, 1492, 1868, 2301
MvnI CGCG 2 cut(s) 305, 659
NarI GGCGCC 3 cut(s) 240, 485, 662
NciI CCSGG 4 cut(s) 309, 689, 1014, 2050
NcoI CCATGG 3 cut(s) 906, 1374, 2294
NlaIV GGNNCC 9 cut(s) 241, 486, 663, 727, 826, 1018, 1428, 1762, 2305
NmeAIII GCCGAG 2 cut(s) 274, 690
NmuCI GTSAC 6 cut(s) 973, 1837, 2032, 2080, 2315, 2722
NsiI ATGCAT 1 cut(s) 2638
NspI RCATGY 1 cut(s) 1851
NspV TTCGAA 3 cut(s) 87, 1572, 2104
PaeI GCATGC 1 cut(s) 1851
PaeR7I CTCGAG 2 cut(s) 212, 2208
PagI TCATGA 3 cut(s) 1261, 1828, 2254
PciSI GCTCTTC 2 cut(s) 1492, 2196
PcsI WCGNNNNNNNCGW 6 cut(s) 60, 524, 567, 650, 764, 924
PctI GAATGC 1 cut(s) 2341
PdmI GAANNNNTTC 4 cut(s) 679, 872, 2262, 2545
PfeI GAWTC 7 cut(s) 89, 811, 1252, 2045, 2053, 2546, 2684
PflFI GACNNNGTC 3 cut(s) 377, 646, 1517
PflMI CCANNNNNTGG 1 cut(s) 2300
PfoI TCCNGGA 2 cut(s) 1490, 2048
Ple19I CGATCG 2 cut(s) 657, 1424
PleI GAGTC 6 cut(s) 71, 631, 1980, 2168, 2569, 2603
PluTI GGCGCC 3 cut(s) 243, 488, 665
PpsI GAGTC 6 cut(s) 71, 631, 1980, 2168, 2569, 2603
PpuMI RGGWCCY 2 cut(s) 33, 2804
Psp1406I AACGTT 1 cut(s) 2365
Psp5II RGGWCCY 2 cut(s) 33, 2804
Psp6I CCWGG 5 cut(s) 728, 1245, 1490, 1866, 2299
PspEI GGTNACC 1 cut(s) 2315
PspGI CCWGG 5 cut(s) 728, 1245, 1490, 1866, 2299
PspN4I GGNNCC 9 cut(s) 241, 486, 663, 727, 826, 1018, 1428, 1762, 2305
PspPI GGNCC 9 cut(s) 33, 223, 732, 1159, 1427, 1597, 1608, 2664, 2804
PspPPI RGGWCCY 2 cut(s) 33, 2804
PspXI VCTCGAGB 2 cut(s) 212, 2208
PstNI CAGNNNCTG 1 cut(s) 2393
PsuI RGATCY 2 cut(s) 1822, 2521
PsyI GACNNNGTC 3 cut(s) 377, 646, 1517
PvuI CGATCG 2 cut(s) 657, 1424
PvuII CAGCTG 1 cut(s) 1621
RsaI GTAC 9 cut(s) 546, 686, 848, 935, 1593, 1910, 2153, 2376, 2600
RsaNI GTAC 9 cut(s) 545, 685, 847, 934, 1592, 1909, 2152, 2375, 2599
RseI CAYNNNNRTG 4 cut(s) 296, 674, 1340, 1584
SalI GTCGAC 2 cut(s) 649, 766
SapI GCTCTTC 2 cut(s) 1492, 2196
SaqAI TTAA 4 cut(s) 387, 807, 1284, 1931
Sau96I GGNCC 9 cut(s) 33, 223, 732, 1159, 1427, 1597, 1608, 2664, 2804
SchI GAGTC 6 cut(s) 71, 631, 1981, 2168, 2570, 2603
ScrFI CCNGG 9 cut(s) 309, 689, 730, 1014, 1247, 1492, 1868, 2050, 2301
SduI GDGCHC 4 cut(s) 671, 1271, 1568, 2237
SfaNI GCATC 9 cut(s) 330, 402, 948, 1288, 1408, 1576, 1759, 2405, 2645
SfoI GGCGCC 3 cut(s) 241, 486, 663
Sfr274I CTCGAG 2 cut(s) 212, 2208
SfuI TTCGAA 3 cut(s) 87, 1572, 2104
SgrAI CRCCGGYG 1 cut(s) 940
SgrDI CGTCGACG 1 cut(s) 649
SinI GGWCC 7 cut(s) 33, 223, 1159, 1597, 1608, 2664, 2804
SlaI CTCGAG 2 cut(s) 212, 2208
SmiMI CAYNNNNRTG 4 cut(s) 296, 674, 1340, 1584
SmlI CTYRAG 2 cut(s) 212, 2208
SmoI CTYRAG 2 cut(s) 212, 2208
SphI GCATGC 1 cut(s) 1851
SspDI GGCGCC 3 cut(s) 239, 484, 661
SspI AATATT 2 cut(s) 2261, 2794
SspMI CTAG 6 cut(s) 744, 1302, 1314, 2166, 2460, 2812
StyD4I CCNGG 9 cut(s) 307, 687, 728, 1012, 1245, 1490, 1866, 2048, 2299
StyI CCWWGG 3 cut(s) 906, 1374, 2294
TaaI ACNGT 6 cut(s) 549, 1651, 1725, 1925, 2427, 2603
TaiI ACGT 2 cut(s) 647, 2368
TatI WGTACW 4 cut(s) 1591, 1908, 2151, 2374
TfiI GAWTC 7 cut(s) 89, 811, 1252, 2045, 2053, 2546, 2684
Tru1I TTAA 4 cut(s) 387, 807, 1284, 1931
Tru9I TTAA 4 cut(s) 387, 807, 1284, 1931
TscAI CASTG 6 cut(s) 883, 1401, 1846, 1885, 2273, 2608
TseFI GTSAC 6 cut(s) 973, 1837, 2032, 2080, 2315, 2722
TseI GCWGC 7 cut(s) 1150, 1844, 1871, 2477, 2483, 2571, 2574
Tsp45I GTSAC 6 cut(s) 973, 1837, 2032, 2080, 2315, 2722
TspDTI ATGAA 9 cut(s) 59, 823, 1014, 1328, 1620, 1817, 1845, 2271, 2697
TspGWI ACGGA 8 cut(s) 261, 576, 607, 735, 785, 916, 2525, 2558
TspRI CASTG 6 cut(s) 883, 1401, 1846, 1885, 2273, 2608
Tth111I GACNNNGTC 3 cut(s) 377, 646, 1517
Van91I CCANNNNNTGG 1 cut(s) 2300
VpaK11BI GGWCC 7 cut(s) 33, 223, 1159, 1597, 1608, 2664, 2804
XagI CCTNNNNNAGG 1 cut(s) 2593
XapI RAATTY 3 cut(s) 520, 1532, 2761
XbaI TCTAGA 1 cut(s) 743
XceI RCATGY 1 cut(s) 1851
XhoI CTCGAG 2 cut(s) 212, 2208
XmiI GTMKAC 4 cut(s) 650, 767, 1812, 2770
XmnI GAANNNNTTC 4 cut(s) 679, 872, 2262, 2545
XspI CTAG 6 cut(s) 744, 1302, 1314, 2166, 2460, 2812
ZraI GACGTC 1 cut(s) 645
Zsp2I ATGCAT 1 cut(s) 2638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.