Rh3DG152700

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0012301
Physical Location & Seq
Forward (+)
13145057 .. 13148664
3608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG152700.1

Sequence Viewer

Length: 2742 bp
ATGATCATGGAGCCGAAGATGGAGCGGAAGAGGACGTTCGCTATGAACTGGGACGGCCTCCACGACGAAGAAGACGACGACCGCTTCTTCGAGTCCCGGGAGAGAATCTCCTCCGCCGTGCCCATCGACCTCGAAGCCTCCTCCTCCGACGATGAGGACTTCGACGACAGTCGTATGTCCTTCGCCTCCTGCGTCTCCTCCGTCCGAAACGACGTCGAGGTGCTGCGAGCCTTGTCCGCCGCCGCCGCCGCCGAGCCGTCCTTCGCCTCGTCGCCTCCGATGTCACCGGACTACAACATCTGGATGGCCGCGCCGGGGTCGATCTCCGAACGCCGCAGGCGGTTGTTGCAGGGCATGGGATTGGCCAACGGCGGCAAAGACATCGTCAGCTTCAGACGCCTCGTTTCGAATAAAGCCCCAAACGGCGTCGTTCCGGCGGTTACGAGTTGCCCTACTCGCTCTCGGGAAAAGATACAAGTTCCCGAAACGGCGCCCGAACCCGATCCCGATCCGGAGCCGGAGTCGGGGGCTTCGAATTCGCCGTTGCCGATGATGCTTGTACGGTCCAGATCGGAAGGAGAGATCGAAGCTTTCTCGGCCGATAAACGAAGGAAAGAGGAGGTAATTGGCACTGTCTCGAAACAACGGCTGACAAGAACGTCGTCGGCGATCGCGGCGCCTAGCTCTAGGATGTGTTTTTCCACGACGCCTCAAAGCGACGGAGGAACCAAGACCGTCCGATCTAGGCAAAACAGCGCCGCATTGTCGACGGAGTTCACCAACAATTTAGGGGCGTTCTTCTTGATTAAAAATCTGGACACGGGAACCGAGTTCATTGTGAACGAGTACGATCAAAACGGGTGTTGGAACAAGTTTAGTGATCTCCAAACGGGGAAGAAATTGACCATGGACGAGTTTGAGAAGTCCGTCGGGTACTCGCCGGTGGTGAAGGAGGTGATGCGGCGGCAAAATGTCAACATTGAAGCCGGGATTGAGAGGAAGGTACCGGCAGGAGCCAATTCGTTTGTGTCAAAGAGTTTGAGGATGAGCAAGAGAAAGGTGGCTCTTTTGAAGAATAGCATAAAAGGTGTGGGAAGTGCTGTGAGTGTACTAATTGGGGATAAGGATCAACACGCCTCGCCGCCGCCGCCGCCGCTGCCGCAGGACCAGAAATCAAGCAAGGCTAATTCGGGGTCTGGTTCGGAGTGGATCAAAGTTAGGCAGACTGGGAAGTCATATAAGGAGCTATCAGCATTGCATTTGTGCCAGGAGATTCAGGCTCATGAGGGCTCAATTTGGACCATGAAGTTTAGCTTGGATGCTAGGTTTCTTGCTAGTGCTGGAGAGGACAGGGTCATTCATGTGTGGGAAGTTCAAGAATGTGAGATTATGTCCATGGATAGCAATGCGAACCCGCTGCATCCCTCCTCGGTGTCCCCATATCCGGATCGATCAATGTGTCTTAGCTCTGAAGCTACAATCTCCTCTGAGAAGAAGAAGAAGGGGAAGGGAGGCTCTTCTAGGAAAACTAATCCCATTCCTGACTATGTCCATGTACCCGAATTCGTGTTTGCCCTCTCGGAGAAACCTGTGTACTCTTTCGAAGGTCATTTGGATGCAGTACTGGACCTGTCATGGTCCAGAGATCAGTTTAACATGGGAATCGGTCTGGCAGTAACTTGCATACAGTTCAATCCATTGGACGACGGTGTTTTTCTCAGCGGCTCCCTTGATGCGAAGCTTCGATTATGGGACATACCTGATCGAAGAGTGGTGGACTGGACTGATCTTCATGAAATGGTCACCGCTGCATGCTATACTCCAGATGGCCAGGTCCAGCTCAATTCTATTATATATGATTGTAAATTAAGTCAAGAAGGCCAGATTGATATTAAAAACAAGAAGAAGAATCAAGCAAAGAAAATTACCGGTTTCCAGTTTTCTCCGATTAATCCATCTGAAATGCTTGTAACATCTGCTGATTCCCGGATTAGGATTGTGGACGGTAAAGATGTCACTCATAAGTTTAGAGGTTTCCGAAATACAAGCAGTCAAATTGGAGCGTCATTCACTCCCAATGGAAAGTACATTATATGTGCCAGTGAAGATTCTCATGTATATGTATGGAAGCGTGAAGAGCCTCGAGTGTCAGCCGCAGGAAAAAAGAGCACAATCACCACCACTTCTCATGAATATTTTCAGTGTAAAGATGTTTCAGTAGCAATCCCATGGCCTGGCACCATAAAAGGTGACCCTCCCCCGCCTGTGTCTGTGCATTCTTCTAAGAGGCACTCAAAACGTTCCTCTGTACAGCCAGTTTCAGGAACTGCATCACCTACTCAAGACGGAGGAGACAGCAAGAGATTCCTGCCACCTCTTCCAAAGAAAAACACTAATCCTAGTACTCCTCCTAATGCGACAGCGGAGAAACCTTCAACTCCCCCACCAGAAGATCAGGATCCATCTGAAATTTCTCGAACGGATTCTGGGATTGGCGAGTCATTCAGCTCAGACCCTTCCTCTATTAGGTATGGTGGCGATTCACCTTCTATGTCTTCTGGCAATGCATCATCATCTTGGTCTTCCTCTTGGTCCTCTTTGTTTGGGCATGAATCGAGCCAAACAACCCAAGCTTGGGGCTTGGTCATTGTGACAGCCACTCTGGAAGGTGAAATCAGGGCATATCAAAATTTTGGGATACCAAGAAGGGTGCCCGGCAGTTTTTTTGGAGGTCCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

913

Amino Acids

99.92

Weight (kDa)

6.77

Isoelectric Point (pI)

55.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 417 - 464 9.5e-07 THOC3 beta-propeller domain
WD40 PF00400 422 - 456 9.6e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 557 - 713 2.2e-13 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 558 - 718 2.4e-17 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 570 - 715 2.9e-10 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37670 AT2G37670 AT5G02430
fragaria_vesca FvH4_6g13960
malus_domestica MD04G1127300.v1.1 MD12G1140500.v1.1
prunus_persica Prupe.6G253900_v2.0.a1 Prupe.6G253900_v2.0.a1
pyrus_communis pycom04g11410
rosa_chinensis RchiOBHm_Chr3g0466781
rosa_laevigata RLG00000024553
rosa_multiflora Rmu_sc0008017.1_g000009
rosa_roxburghii Rroxscaffold_6G00414170
rosa_rugosa Rorug03G0083800
rosa_samantha Rh3AG131000 Rh3BG152000 Rh3CG151900 Rh3DG152700
rosa_wichuraiana Rw3G012380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 658, 1231
AatII GACGTC 1 cut(s) 216
Acc65I GGTACC 1 cut(s) 1003
AccB1I GGYRCC 5 cut(s) 490, 676, 1003, 2237, 2710
AccB7I CCANNNNNTGG 1 cut(s) 2234
AccBSI CCGCTC 1 cut(s) 25
AccI GTMKAC 1 cut(s) 767
AccII CGCG 2 cut(s) 311, 674
AccIII TCCGGA 2 cut(s) 511, 1444
AclI AACGTT 1 cut(s) 2299
AclWI GGATC 7 cut(s) 497, 503, 1134, 1217, 1455, 2453, 2466
AcoI YGGCCR 4 cut(s) 306, 363, 597, 1828
AcsI RAATTY 4 cut(s) 535, 1562, 2469, 2689
AcuI CTGAAG 2 cut(s) 376, 1491
AcyI GRCGYC 6 cut(s) 213, 397, 426, 491, 677, 707
AfiI CCNNNNNNNGG 9 cut(s) 315, 524, 1444, 1992, 2234, 2321, 2526, 2634, 2635
AgeI ACCGGT 1 cut(s) 1928
AgsI TTSAA 5 cut(s) 983, 1072, 1376, 1693, 2436
AjnI CCWGG 3 cut(s) 1266, 1830, 2233
AjuI GAANNNNNNNTTGG 2 cut(s) 1298, 1330
Alw21I GWGCWC 1 cut(s) 2171
Alw26I GTCTC 3 cut(s) 199, 640, 2346
AlwI GGATC 7 cut(s) 497, 503, 1134, 1217, 1455, 2453, 2466
AlwNI CAGNNNCTG 1 cut(s) 2327
Ama87I CYCGRG 3 cut(s) 96, 462, 2142
Aor13HI TCCGGA 2 cut(s) 511, 1444
AoxI GGCC 7 cut(s) 55, 306, 363, 597, 1828, 1879, 2231
ApeKI GCWGC 4 cut(s) 223, 1156, 1417, 1808
ApoI RAATTY 4 cut(s) 535, 1562, 2469, 2689
ArsI GACNNNNNNTTYG 4 cut(s) 71, 103, 369, 401
AseI ATTAAT 1 cut(s) 1950
AsiGI ACCGGT 1 cut(s) 1928
AsiSI GCGATCGC 1 cut(s) 672
Asp700I GAANNNNTTC 2 cut(s) 2196, 2482
Asp718I GGTACC 1 cut(s) 1003
AspLEI GCGC 4 cut(s) 313, 493, 679, 758
AspS9I GGNCC 8 cut(s) 564, 1165, 1299, 1627, 1638, 1834, 2592, 2732
AsuC2I CCSGG 6 cut(s) 97, 98, 315, 988, 1987, 2715
AsuII TTCGAA 3 cut(s) 407, 533, 1602
AvaI CYCGRG 3 cut(s) 96, 462, 2142
AvaII GGWCC 8 cut(s) 564, 1165, 1299, 1627, 1638, 1834, 2592, 2732
BaeGI GKGCMC 2 cut(s) 123, 2715
BalI TGGCCA 2 cut(s) 365, 1830
BamHI GGATCC 1 cut(s) 2458
BanI GGYRCC 5 cut(s) 490, 676, 1003, 2237, 2710
BanII GRGCYC 1 cut(s) 1292
BbsI GAAGAC 3 cut(s) 78, 2547, 2574
Bbv12I GWGCWC 1 cut(s) 2171
BbvI GCAGC 4 cut(s) 210, 1143, 1404, 1795
BccI CCATC 6 cut(s) 13, 131, 298, 1820, 1963, 2470
BceAI ACGGC 8 cut(s) 70, 101, 241, 385, 439, 504, 526, 662
BcgI CGANNNNNNTGC 4 cut(s) 364, 398, 1399, 1433
BciT130I CCWGG 3 cut(s) 1268, 1832, 2235
BciVI GTATCC 1 cut(s) 2691
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 6 cut(s) 97, 98, 315, 988, 1987, 2715
BcoDI GTCTC 3 cut(s) 199, 640, 2346
BfaI CTAG 8 cut(s) 681, 687, 744, 1323, 1335, 1521, 2400, 2740
BfoI RGCGCY 3 cut(s) 494, 680, 759
BfuI GTATCC 1 cut(s) 2691
BmcAI AGTACT 2 cut(s) 1623, 2404
Bme1390I CCNGG 9 cut(s) 97, 98, 315, 988, 1268, 1832, 1987, 2235, 2715
Bme18I GGWCC 8 cut(s) 564, 1165, 1299, 1627, 1638, 1834, 2592, 2732
BmeT110I CYCGRG 3 cut(s) 96, 462, 2142
BmgT120I GGNCC 8 cut(s) 564, 1165, 1299, 1627, 1638, 1834, 2592, 2732
BmrFI CCNGG 9 cut(s) 97, 98, 315, 988, 1268, 1832, 1987, 2235, 2715
BmrI ACTGGG 2 cut(s) 58, 1236
BmsI GCATC 8 cut(s) 543, 948, 1309, 1429, 1606, 1723, 2339, 2576
BmuI ACTGGG 2 cut(s) 58, 1236
BoxI GACNNNNGTC 1 cut(s) 168
BpiI GAAGAC 3 cut(s) 78, 2547, 2574
BplI GAGNNNNNCTC 2 cut(s) 92, 124
BpmI CTGGAG 2 cut(s) 1362, 1806
Bpu14I TTCGAA 3 cut(s) 407, 533, 1602
BpuEI CTTGAG 1 cut(s) 2325
BpuMI CCSGG 6 cut(s) 97, 98, 315, 988, 1987, 2715
Bsa29I ATCGAT 1 cut(s) 1450
BsaBI GATNNNNATC 3 cut(s) 507, 1125, 2457
BsaHI GRCGYC 6 cut(s) 213, 397, 426, 491, 677, 707
BsaJI CCNNGG 6 cut(s) 96, 314, 906, 1395, 1428, 2228
BsaWI WCCGGW 4 cut(s) 286, 511, 1444, 1928
BsaXI ACNNNNNCTCC 2 cut(s) 506, 536
Bsc4I CCNNNNNNNGG 9 cut(s) 315, 524, 1444, 1992, 2234, 2321, 2526, 2634, 2635
Bse118I RCCGGY 3 cut(s) 940, 1006, 1928
Bse1I ACTGG 7 cut(s) 53, 1231, 1629, 1784, 1936, 2100, 2315
Bse3DI GCAATG 3 cut(s) 1253, 1411, 2569
Bse8I GATNNNNATC 3 cut(s) 507, 1125, 2457
BseAI TCCGGA 2 cut(s) 511, 1444
BseBI CCWGG 3 cut(s) 1268, 1832, 2235
BseCI ATCGAT 1 cut(s) 1450
BseDI CCNNGG 6 cut(s) 96, 314, 906, 1395, 1428, 2228
BseGI GGATG 6 cut(s) 309, 696, 1050, 1324, 1420, 1621
BseJI GATNNNNATC 3 cut(s) 507, 1125, 2457
BseLI CCNNNNNNNGG 9 cut(s) 315, 524, 1444, 1992, 2234, 2321, 2526, 2634, 2635
BseMI GCAATG 3 cut(s) 1253, 1411, 2569
BseMII CTCAG 3 cut(s) 1479, 1732, 2523
BseNI ACTGG 7 cut(s) 53, 1231, 1629, 1784, 1936, 2100, 2315
BseRI GAGGAG 9 cut(s) 100, 130, 133, 187, 632, 1417, 1474, 2364, 2397
BseSI GKGCMC 2 cut(s) 123, 2715
BseX3I CGGCCG 1 cut(s) 597
BseXI GCAGC 4 cut(s) 210, 1143, 1404, 1795
Bsh1236I CGCG 2 cut(s) 311, 674
Bsh1285I CGRYCG 3 cut(s) 82, 600, 672
BshFI GGCC 7 cut(s) 57, 308, 365, 599, 1830, 1881, 2233
BshNI GGYRCC 5 cut(s) 490, 676, 1003, 2237, 2710
BshTI ACCGGT 1 cut(s) 1928
BshVI ATCGAT 1 cut(s) 1450
BsiEI CGRYCG 3 cut(s) 82, 600, 672
BsiHKAI GWGCWC 1 cut(s) 2171
BsiHKCI CYCGRG 3 cut(s) 96, 462, 2142
BslFI GGGAC 4 cut(s) 65, 79, 1420, 1766
BslI CCNNNNNNNGG 9 cut(s) 315, 524, 1444, 1992, 2234, 2321, 2526, 2634, 2635
BsmAI GTCTC 3 cut(s) 199, 640, 2346
BsmBI CGTCTC 1 cut(s) 199
BsmFI GGGAC 4 cut(s) 65, 79, 1420, 1766
BsmI GAATGC 1 cut(s) 2275
BsnI GGCC 7 cut(s) 57, 308, 365, 599, 1830, 1881, 2233
BsoBI CYCGRG 3 cut(s) 96, 462, 2142
Bsp119I TTCGAA 3 cut(s) 407, 533, 1602
Bsp1286I GDGCHC 4 cut(s) 123, 1292, 2171, 2715
Bsp13I TCCGGA 2 cut(s) 511, 1444
Bsp1407I TGTACA 1 cut(s) 2308
Bsp19I CCATGG 3 cut(s) 906, 1395, 2228
BspANI GGCC 7 cut(s) 57, 308, 365, 599, 1830, 1881, 2233
BspCNI CTCAG 3 cut(s) 1480, 1731, 2522
BspDI ATCGAT 1 cut(s) 1450
BspEI TCCGGA 2 cut(s) 511, 1444
BspFNI CGCG 2 cut(s) 311, 674
BspHI TCATGA 3 cut(s) 1282, 1792, 2188
BspPI GGATC 7 cut(s) 497, 503, 1134, 1217, 1455, 2453, 2466
BspQI GCTCTTC 2 cut(s) 1522, 2130
BspT104I TTCGAA 3 cut(s) 407, 533, 1602
BspT107I GGYRCC 5 cut(s) 490, 676, 1003, 2237, 2710
BsrBI CCGCTC 1 cut(s) 25
BsrDI GCAATG 3 cut(s) 1253, 1411, 2569
BsrFI RCCGGY 3 cut(s) 940, 1006, 1928
BsrGI TGTACA 1 cut(s) 2308
BsrI ACTGG 7 cut(s) 53, 1231, 1629, 1784, 1936, 2100, 2315
BssAI RCCGGY 3 cut(s) 940, 1006, 1928
BssECI CCNNGG 6 cut(s) 96, 314, 906, 1395, 1428, 2228
BssNI GRCGYC 6 cut(s) 213, 397, 426, 491, 677, 707
BssT1I CCWWGG 3 cut(s) 906, 1395, 2228
Bst2UI CCWGG 3 cut(s) 1268, 1832, 2235
Bst4CI ACNGT 7 cut(s) 170, 564, 634, 736, 1689, 1709, 2006
Bst6I CTCTTC 5 cut(s) 23, 1522, 1762, 2130, 2382
BstACI GRCGYC 6 cut(s) 213, 397, 426, 491, 677, 707
BstAUI TGTACA 1 cut(s) 2308
BstBI TTCGAA 3 cut(s) 407, 533, 1602
BstC8I GCNNGC 3 cut(s) 228, 338, 1813
BstDEI CTNAG 5 cut(s) 1463, 1488, 1718, 2283, 2509
BstDSI CCRYGG 3 cut(s) 906, 1395, 2228
BstEII GGTNACC 2 cut(s) 1801, 2249
BstENI CCTNNNNNAGG 1 cut(s) 2524
BstF5I GGATG 6 cut(s) 309, 696, 1050, 1324, 1420, 1621
BstFNI CGCG 2 cut(s) 311, 674
BstH2I RGCGCY 3 cut(s) 494, 680, 759
BstHHI GCGC 4 cut(s) 313, 493, 679, 758
BstMAI GTCTC 3 cut(s) 199, 640, 2346
BstMCI CGRYCG 3 cut(s) 82, 600, 672
BstNI CCWGG 3 cut(s) 1268, 1832, 2235
BstNSI RCATGY 1 cut(s) 1815
BstPAI GACNNNNGTC 1 cut(s) 168
BstPI GGTNACC 2 cut(s) 1801, 2249
BstSCI CCNGG 9 cut(s) 95, 96, 313, 986, 1266, 1830, 1985, 2233, 2713
BstSLI GKGCMC 2 cut(s) 123, 2715
BstUI CGCG 2 cut(s) 311, 674
BstV1I GCAGC 4 cut(s) 210, 1143, 1404, 1795
BstV2I GAAGAC 3 cut(s) 78, 2547, 2574
BstX2I RGATCY 1 cut(s) 2458
BstYI RGATCY 1 cut(s) 2458
BstZI CGGCCG 1 cut(s) 597
Bsu15I ATCGAT 1 cut(s) 1450
BsuI GTATCC 1 cut(s) 2691
BsuRI GGCC 7 cut(s) 57, 308, 365, 599, 1830, 1881, 2233
BsuTUI ATCGAT 1 cut(s) 1450
BtgI CCRYGG 3 cut(s) 906, 1395, 2228
BtsCI GGATG 6 cut(s) 309, 696, 1050, 1324, 1420, 1621
BtsIMutI CAGTG 3 cut(s) 630, 2107, 2207
Cac8I GCNNGC 3 cut(s) 228, 338, 1813
CaiI CAGNNNCTG 1 cut(s) 2327
CciI TCATGA 3 cut(s) 1282, 1792, 2188
CfoI GCGC 4 cut(s) 313, 493, 679, 758
Cfr10I RCCGGY 3 cut(s) 940, 1006, 1928
Cfr13I GGNCC 8 cut(s) 564, 1165, 1299, 1627, 1638, 1834, 2592, 2732
Cfr9I CCCGGG 1 cut(s) 96
ClaI ATCGAT 1 cut(s) 1450
CseI GACGC 5 cut(s) 181, 405, 415, 715, 2052
CspAI ACCGGT 1 cut(s) 1928
DdeI CTNAG 5 cut(s) 1463, 1488, 1718, 2283, 2509
DinI GGCGCC 2 cut(s) 492, 678
DrdI GACNNNNNNGTC 2 cut(s) 658, 1231
DseDI GACNNNNNNGTC 2 cut(s) 658, 1231
EaeI YGGCCR 4 cut(s) 306, 363, 597, 1828
EagI CGGCCG 1 cut(s) 597
Eam1104I CTCTTC 5 cut(s) 23, 1522, 1762, 2130, 2382
EarI CTCTTC 5 cut(s) 23, 1522, 1762, 2130, 2382
EciI GGCGGA 2 cut(s) 103, 226
EclXI CGGCCG 1 cut(s) 597
Eco130I CCWWGG 3 cut(s) 906, 1395, 2228
Eco24I GRGCYC 1 cut(s) 1292
Eco47I GGWCC 8 cut(s) 564, 1165, 1299, 1627, 1638, 1834, 2592, 2732
Eco52I CGGCCG 1 cut(s) 597
Eco57I CTGAAG 2 cut(s) 376, 1491
Eco88I CYCGRG 3 cut(s) 96, 462, 2142
Eco91I GGTNACC 2 cut(s) 1801, 2249
EcoNI CCTNNNNNAGG 1 cut(s) 2524
EcoO109I RGGNCCY 1 cut(s) 2732
EcoO65I GGTNACC 2 cut(s) 1801, 2249
EcoRI GAATTC 2 cut(s) 535, 1562
EcoRII CCWGG 3 cut(s) 1266, 1830, 2233
EcoT14I CCWWGG 3 cut(s) 906, 1395, 2228
EcoT22I ATGCAT 1 cut(s) 2569
EcoT38I GRGCYC 1 cut(s) 1292
EgeI GGCGCC 2 cut(s) 492, 678
EheI GGCGCC 2 cut(s) 492, 678
ErhI CCWWGG 3 cut(s) 906, 1395, 2228
Esp3I CGTCTC 1 cut(s) 199
FalI AAGNNNNNCTT 2 cut(s) 1298, 1330
FaqI GGGAC 4 cut(s) 65, 79, 1420, 1766
FauI CCCGC 2 cut(s) 1422, 2268
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 767
FokI GGATG 6 cut(s) 316, 703, 1057, 1331, 1407, 1628
FriOI GRGCYC 1 cut(s) 1292
FspBI CTAG 8 cut(s) 681, 687, 744, 1323, 1335, 1521, 2400, 2740
GlaI GCGC 4 cut(s) 312, 492, 678, 757
GsuI CTGGAG 2 cut(s) 1362, 1806
HaeII RGCGCY 3 cut(s) 494, 680, 759
HaeIII GGCC 7 cut(s) 57, 308, 365, 599, 1830, 1881, 2233
HgaI GACGC 5 cut(s) 181, 405, 415, 715, 2052
HhaI GCGC 4 cut(s) 313, 493, 679, 758
Hin1I GRCGYC 6 cut(s) 213, 397, 426, 491, 677, 707
Hin6I GCGC 4 cut(s) 311, 491, 677, 756
HinP1I GCGC 4 cut(s) 311, 491, 677, 756
HincII GTYRAC 2 cut(s) 768, 976
HindII GTYRAC 2 cut(s) 768, 976
HindIII AAGCTT 3 cut(s) 588, 1739, 2631
Hpy166II GTNNAC 8 cut(s) 768, 777, 841, 976, 1109, 1594, 1777, 2002
Hpy8I GTNNAC 8 cut(s) 768, 777, 841, 976, 1109, 1594, 1777, 2002
HpyCH4III ACNGT 7 cut(s) 170, 564, 634, 736, 1689, 1709, 2006
HpyCH4IV ACGT 4 cut(s) 35, 213, 659, 2299
HpyCH4V TGCA 9 cut(s) 349, 1258, 1420, 1619, 1683, 1811, 2275, 2330, 2567
HpyF3I CTNAG 5 cut(s) 1463, 1488, 1718, 2283, 2509
HpySE526I ACGT 4 cut(s) 35, 213, 659, 2299
Hsp92I GRCGYC 6 cut(s) 213, 397, 426, 491, 677, 707
HspAI GCGC 4 cut(s) 311, 491, 677, 756
KasI GGCGCC 2 cut(s) 490, 676
Kpn2I TCCGGA 2 cut(s) 511, 1444
KpnI GGTACC 1 cut(s) 1007
Ksp22I TGATCA 1 cut(s) 3
LguI GCTCTTC 2 cut(s) 1522, 2130
LmnI GCTCC 7 cut(s) 10, 22, 514, 1013, 1243, 1730, 2060
Lsp1109I GCAGC 4 cut(s) 210, 1143, 1404, 1795
LweI GCATC 8 cut(s) 543, 948, 1309, 1429, 1606, 1723, 2339, 2576
MaeI CTAG 8 cut(s) 681, 687, 744, 1323, 1335, 1521, 2400, 2740
MaeII ACGT 4 cut(s) 35, 213, 659, 2299
MaeIII GTNAC 8 cut(s) 282, 439, 1675, 1801, 1969, 2014, 2249, 2650
MbiI CCGCTC 1 cut(s) 25
MflI RGATCY 1 cut(s) 2458
MhlI GDGCHC 4 cut(s) 123, 1292, 2171, 2715
MlsI TGGCCA 2 cut(s) 365, 1830
MluNI TGGCCA 2 cut(s) 365, 1830
Mly113I GGCGCC 2 cut(s) 491, 677
MlyI GAGTC 3 cut(s) 101, 530, 2507
MmeI TCCRAC 2 cut(s) 171, 845
Mox20I TGGCCA 2 cut(s) 365, 1830
Mph1103I ATGCAT 1 cut(s) 2569
MroI TCCGGA 2 cut(s) 511, 1444
MroXI GAANNNNTTC 2 cut(s) 2196, 2482
MscI TGGCCA 2 cut(s) 365, 1830
MseI TTAA 5 cut(s) 807, 1653, 1868, 1893, 1950
MslI CAYNNNNRTG 4 cut(s) 302, 1361, 1614, 2118
Msp20I TGGCCA 2 cut(s) 365, 1830
MspA1I CMGCKG 5 cut(s) 1156, 1417, 1722, 1808, 2423
MspR9I CCNGG 9 cut(s) 97, 98, 315, 988, 1268, 1832, 1987, 2235, 2715
Mva1269I GAATGC 1 cut(s) 2275
MvaI CCWGG 3 cut(s) 1268, 1832, 2235
MvnI CGCG 2 cut(s) 311, 674
NarI GGCGCC 2 cut(s) 491, 677
NciI CCSGG 6 cut(s) 97, 98, 315, 988, 1987, 2715
NcoI CCATGG 3 cut(s) 906, 1395, 2228
NmeAIII GCCGAG 2 cut(s) 277, 575
NmuCI GTSAC 5 cut(s) 282, 1801, 2014, 2249, 2650
NsiI ATGCAT 1 cut(s) 2569
NspI RCATGY 1 cut(s) 1815
NspV TTCGAA 3 cut(s) 407, 533, 1602
PaeI GCATGC 1 cut(s) 1815
PaeR7I CTCGAG 1 cut(s) 2142
PagI TCATGA 3 cut(s) 1282, 1792, 2188
PciSI GCTCTTC 2 cut(s) 1522, 2130
PcsI WCGNNNNNNNCGW 8 cut(s) 60, 72, 189, 275, 530, 539, 545, 665
PctI GAATGC 1 cut(s) 2275
PdmI GAANNNNTTC 2 cut(s) 2196, 2482
PflFI GACNNNGTC 3 cut(s) 383, 1352, 1547
PflMI CCANNNNNTGG 1 cut(s) 2234
PfoI TCCNGGA 1 cut(s) 1985
PinAI ACCGGT 1 cut(s) 1928
Ple19I CGATCG 1 cut(s) 672
PleI GAGTC 3 cut(s) 100, 529, 2506
PluTI GGCGCC 2 cut(s) 494, 680
PpsI GAGTC 3 cut(s) 100, 529, 2506
PpuMI RGGWCCY 1 cut(s) 2732
PshAI GACNNNNGTC 1 cut(s) 168
PshBI ATTAAT 1 cut(s) 1950
Psp1406I AACGTT 1 cut(s) 2299
Psp5II RGGWCCY 1 cut(s) 2732
Psp6I CCWGG 3 cut(s) 1266, 1830, 2233
PspEI GGTNACC 2 cut(s) 1801, 2249
PspGI CCWGG 3 cut(s) 1266, 1830, 2233
PspPI GGNCC 8 cut(s) 564, 1165, 1299, 1627, 1638, 1834, 2592, 2732
PspPPI RGGWCCY 1 cut(s) 2732
PspXI VCTCGAGB 1 cut(s) 2142
PstNI CAGNNNCTG 1 cut(s) 2327
PsuI RGATCY 1 cut(s) 2458
PsyI GACNNNGTC 3 cut(s) 383, 1352, 1547
PvuI CGATCG 1 cut(s) 672
RgaI GCGATCGC 1 cut(s) 672
RseI CAYNNNNRTG 4 cut(s) 302, 1361, 1614, 2118
SalI GTCGAC 1 cut(s) 766
SapI GCTCTTC 2 cut(s) 1522, 2130
SaqAI TTAA 5 cut(s) 807, 1653, 1868, 1893, 1950
Sau96I GGNCC 8 cut(s) 564, 1165, 1299, 1627, 1638, 1834, 2592, 2732
ScaI AGTACT 2 cut(s) 1623, 2404
SchI GAGTC 3 cut(s) 101, 530, 2507
ScrFI CCNGG 9 cut(s) 97, 98, 315, 988, 1268, 1832, 1987, 2235, 2715
SduI GDGCHC 4 cut(s) 123, 1292, 2171, 2715
SfaAI GCGATCGC 1 cut(s) 672
SfaNI GCATC 8 cut(s) 543, 948, 1309, 1429, 1606, 1723, 2339, 2576
SfoI GGCGCC 2 cut(s) 492, 678
Sfr274I CTCGAG 1 cut(s) 2142
SfuI TTCGAA 3 cut(s) 407, 533, 1602
SgfI GCGATCGC 1 cut(s) 672
SgrAI CRCCGGYG 1 cut(s) 940
SinI GGWCC 8 cut(s) 564, 1165, 1299, 1627, 1638, 1834, 2592, 2732
SlaI CTCGAG 1 cut(s) 2142
SmaI CCCGGG 1 cut(s) 98
SmiMI CAYNNNNRTG 4 cut(s) 302, 1361, 1614, 2118
SmlI CTYRAG 2 cut(s) 2142, 2340
SmoI CTYRAG 2 cut(s) 2142, 2340
SphI GCATGC 1 cut(s) 1815
SspDI GGCGCC 2 cut(s) 490, 676
SspI AATATT 1 cut(s) 2195
SspMI CTAG 8 cut(s) 681, 687, 744, 1323, 1335, 1521, 2400, 2740
StyD4I CCNGG 9 cut(s) 95, 96, 313, 986, 1266, 1830, 1985, 2233, 2713
StyI CCWWGG 3 cut(s) 906, 1395, 2228
TaaI ACNGT 7 cut(s) 170, 564, 634, 736, 1689, 1709, 2006
TaiI ACGT 4 cut(s) 38, 216, 662, 2302
TaqII GACCGA 1 cut(s) 1655
TatI WGTACW 6 cut(s) 1108, 1593, 1621, 2085, 2308, 2402
Tru1I TTAA 5 cut(s) 807, 1653, 1868, 1893, 1950
Tru9I TTAA 5 cut(s) 807, 1653, 1868, 1893, 1950
TscAI CASTG 3 cut(s) 637, 2107, 2207
TseFI GTSAC 5 cut(s) 282, 1801, 2014, 2249, 2650
TseI GCWGC 4 cut(s) 223, 1156, 1417, 1808
Tsp45I GTSAC 5 cut(s) 282, 1801, 2014, 2249, 2650
TspDTI ATGAA 8 cut(s) 59, 823, 1319, 1349, 1781, 1809, 2205, 2625
TspGWI ACGGA 6 cut(s) 190, 735, 785, 916, 2361, 2495
TspMI CCCGGG 1 cut(s) 96
TspRI CASTG 3 cut(s) 637, 2107, 2207
Tth111I GACNNNGTC 3 cut(s) 383, 1352, 1547
Van91I CCANNNNNTGG 1 cut(s) 2234
VpaK11BI GGWCC 8 cut(s) 564, 1165, 1299, 1627, 1638, 1834, 2592, 2732
VspI ATTAAT 1 cut(s) 1950
XagI CCTNNNNNAGG 1 cut(s) 2524
XapI RAATTY 4 cut(s) 535, 1562, 2469, 2689
XceI RCATGY 1 cut(s) 1815
XhoI CTCGAG 1 cut(s) 2142
XmaI CCCGGG 1 cut(s) 96
XmiI GTMKAC 1 cut(s) 767
XmnI GAANNNNTTC 2 cut(s) 2196, 2482
XspI CTAG 8 cut(s) 681, 687, 744, 1323, 1335, 1521, 2400, 2740
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 2 cut(s) 1623, 2404
Zsp2I ATGCAT 1 cut(s) 2569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.