MD12G1140500.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012301
Physical Location & Seq
Reverse (-)
21841469 .. 21845726
4258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1140500.v1.1.491

Sequence Viewer

Length: 2874 bp
ATGAAGATGGAGCGGAAGAGGACGTTGACGATGAACTGGGACGGCCTCCGGGACGACGACGACGACGACCGCTTCTTTGAGTCAAATGAACGCGCCTCTTCCGTCATTCCTCTTGACCTTGAGGCATCTTCTTCCGAAGATGAGGATTTTGATGACAGTCGCATGTCATTCACCTCATGCGTTTCCTCTGTTCGAAATGACATCGATTTTAGCGCCTTCGCCACAGCCACTTCGACGAAACCGCCATCCACTGCTGCAGCTCCGATGACACCAGATTACAACATCTGGATGGATACGCCGGGGTCCATCACTGAAAGGAGAAAACGATTGTTGCAGGGCATGGGGTTAGCCCAAGGACCCGGGAAAGACCTTGTCAGCTTTAAGCAGCTAACCAGCATTAAACGCCTCGTTTCGAACAAAATAGCAAATGACGCCATTCCAATCCCCCGGTCCATCTCTCATGCCAAAGAAAATGTACAAGTACCGCCTGATCAAACGGTATCGGAGCCTGAGCATGATTTGTTACCGATGATGCTCGTCCGATCAAGGTCCGAGGGTGACATCGAAGCTTTTTCAGTCGACAAACAGAGGAAAGAGGAGATCATCGGAACAATCTCGAAGCAACGCCTTACGAGGACTTGCTCCACTATCGCCGCTCCTCGTTCAAGAATATGTCCGTATACAGAATCGGTCAAAGGATCGCCAAACCAAGTAGGTGGTGCCACCCCCGTGATCCAATCTGTTCAGACCGGTGGTGGATTATCATCGGTCTTCTCAAACAACCGGTTAGAAGCATTCTTCTTGATCAAGAATCTGGACACGGGGACCGAGTTCATCGTGAACGAGTATGATCAGGATGGGTGCTGGAATCGACTCAGCGATCTCCAAACGGGAAAGCAATTAACGATGGATGAATTTGAGAAATGCGTCGGGTACTCGCCGGTGGTGAAAGAGGTGATGAGAAGACAAAATGTTTCGAGGGAAGGCGGTGGCGACAAGAAAGGCGGCGCAGTCAGTGCAAATTCTTTTGTTTCGAAGAGCCTGAGAATGAGCAAAAGAAGAGGTGTTGCTCTGTTGAAGAATAGTATACGAGGTGTGGGGACTGCCGTGAGTGTGTTGATTGGGGAGAAAGATAAGGAAAACACCACGCCACCGTTGACGCCACCGGCGCAAAAGCCCGCCAAGAACTCGAGTTCTTCTTCCTCAGAATGGGTCAAAGTTAGGCAAACAGGGAAGCATTACAAGGAGCTATCAGCATTGCATCTGTGTCAGGAGATTCAAGCTCACGAGGGGTCGATTTGGAGTATTAAGTTTAGTTTGGATGCGAGGTTTCTTGCGAGTGCAGGAGAGGATCGGGTAATTCATGTGTGGGAAGTGCAAGAATGTGAGATTATGTCATTAGACGGGAATTCGACCCCGCTTCATCACTCTTTCGGGTCCTCTACCCCTGATCGTTCACCATCGATGTCTGATCAAGCTACGCTTGGGCCATCCGATAAGGAGAAGAAGAAGAAGGGGAGAGGAGGGTCTGCTAGAAAAAGCAATTCAATCCCGGAGTACGTGCATGTACCCGAAGCTGTGTTTTCATTTTCGGAGAAACCATTTTGTTCTTTTGAAGGTCATTTGGATGCTGTTTTGGACTTGTCCTGGTCCAGAGATCAGCTATTACTTTCATCTTCAATGGACAAAACTGTTAGATTATGGGATTTGGAGACCAAGACTTGCTTAAAGTTATTTGCCCACAATGATTATGTGACTTGCATACAGTTCAATCCTTTGGATGACAATTACTTTATCAGTGGCTCACTCGATGCCAAGATTCGATTGTGGAATGTAGCCGTTAGGCAAGTCGTGGACTGGACCGATCTTCATGAAATGGTCACTGCTGCTTCCTTCACTCCAGATGGCCAGGCTTCCCTCATTGGTTCGCACAAAGGAAACTGTCATATGTATAGTGCAGAAGATTGTAAATTAAGTCAGCATAATCATATTGATATTCAGAACAAGAAGAAGAATCAAGCCAAAAAGATTACAGGTTTGCAGTTTTCGCCAATGAATCCATCTGAAATGCTTGTTACCTCTGCTGATTCCCGGATTCGAATTTTGGATGGCACAAACGTGACTCATAAGTTTAGAGGTTTCCGAAATACAAGCAGTCAAATTGCAGCTTCATTCACTCCAAACGGAAAATATGTTGTATGTGCTAGTGAAGACTCCCATGTATATGTTTGGAAGCGTGAAGAACCCCGAATCTCAGCCACGGGTAAAAAGAGCATTATCAGTACCAATTCCCACGAGCATTTTCAGTGCAAAGATGTTTCAGCAGCAATACCATGGCCAGGCACCATAAAGGGCGACCCTCCACCTGTCTCCGTGCAACATTCCAAGAGAAACTCAAAACGCTCAACGCTACAGCCGCAATCCGGCAATGCATCCCCTACTCAAGAAGATGCAGGTGTAAGTAAGAGAATGCTACCGCCTCTCCCAAAGAAAAACAACAAAGACAGCAACACCACAACCAATAAGGGAGATGCATCAGATCAAACACCTTCAACTCCTCCGCCGGAAGACCAAGATCCTGCTCAAATCTCTCGAACAGAATCCGGGATTGGAGAGTCGTTTAATTCAGACCCTTCCTCTATTAGGTATGGTGATTCAAGTTCTATGTCTGCTGGTAGTGCCTCCACATCATCTTGGTCTTCCTCTTGGTCATGGCTCGACAATATAGGCAACAGCCATGCTAGCCAAACAACACAAGCAACAGCTTGGGGTTTAGTAATTGTGACAGCCACTCTGGAAGGCGAAATCAGAGCATACCAAAATTTCGGGCTACCGCGAAAGATGCAGAACAATATTTTTGGAGGCCCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

958

Amino Acids

105.26

Weight (kDa)

7.31

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 418 - 463 7.1e-07 THOC3 beta-propeller domain
WD40 PF00400 423 - 457 2.1e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 529 - 651 3e-10 WDR3 second beta-propeller domain
EIF3I PF24805 529 - 734 2.4e-11 EIF3I
Beta-prop_WDR3_1st PF25173 531 - 612 3.3e-14 WDR3 first beta-propeller domain
WD40 PF00400 533 - 569 2.6e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 534 - 755 3e-39 WDR5 beta-propeller domain
WD40_Prp19 PF24814 535 - 757 1.4e-21 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 535 - 620 3.6e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 537 - 595 9.5e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 538 - 748 3.6e-27 THOC3 beta-propeller domain
Beta-prop_IFT122_1st PF23381 538 - 646 3.2e-06 IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 538 - 619 4.1e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 543 - 680 6.2e-10 Aladin seven-bladed propeller
WD40_like PF17005 552 - 747 5.8e-06 WD40-like domain
WD40_Gbeta PF25391 552 - 657 1.4e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 556 - 656 1.1e-08 WDR90/POC16, second beta-propeller
Beta-prop_EML PF23409 581 - 756 6.2e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st PF24817 584 - 748 3.4e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 595 - 748 6.5e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 616 - 747 7e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37670 AT2G37670 AT5G02430
fragaria_vesca FvH4_6g13960
malus_domestica MD04G1127300.v1.1 MD12G1140500.v1.1
prunus_persica Prupe.6G253900_v2.0.a1 Prupe.6G253900_v2.0.a1
pyrus_communis pycom04g11410
rosa_chinensis RchiOBHm_Chr3g0466781
rosa_laevigata RLG00000024553
rosa_multiflora Rmu_sc0008017.1_g000009
rosa_roxburghii Rroxscaffold_6G00414170
rosa_rugosa Rorug03G0083800
rosa_samantha Rh3AG131000 Rh3BG152000 Rh3CG151900 Rh3DG152700
rosa_wichuraiana Rw3G012380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2444
Acc36I ACCTGC 1 cut(s) 2444
AccB1I GGYRCC 2 cut(s) 719, 2342
AccBSI CCGCTC 2 cut(s) 13, 656
AccI GTMKAC 3 cut(s) 579, 680, 1087
AccII CGCG 2 cut(s) 93, 2836
AclWI GGATC 4 cut(s) 706, 727, 1359, 2570
AcoI YGGCCR 2 cut(s) 1906, 2336
AcsI RAATTY 5 cut(s) 914, 1021, 1408, 2100, 2821
AcyI GRCGYC 2 cut(s) 432, 1160
AfaI GTAC 6 cut(s) 477, 483, 935, 1559, 1569, 2284
AfiI CCNNNNNNNGG 4 cut(s) 1209, 2339, 2423, 2643
AgeI ACCGGT 2 cut(s) 749, 783
AgsI TTSAA 9 cut(s) 666, 1078, 1280, 1548, 1616, 1680, 1771, 2553, 2658
AjnI CCWGG 3 cut(s) 1646, 1908, 2338
AjuI GAANNNNNNNTTGG 2 cut(s) 2592, 2624
AleI CACNNNNGTG 2 cut(s) 728, 2117
Alw26I GTCTC 2 cut(s) 1706, 2374
AlwI GGATC 4 cut(s) 706, 727, 1359, 2570
Ama87I CYCGRG 2 cut(s) 359, 1189
AoxI GGCC 5 cut(s) 43, 1487, 1906, 2336, 2863
ApeKI GCWGC 6 cut(s) 254, 257, 385, 1886, 2165, 2324
ApoI RAATTY 5 cut(s) 914, 1021, 1408, 2100, 2821
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AsiGI ACCGGT 2 cut(s) 749, 783
AspLEI GCGC 4 cut(s) 95, 215, 1010, 1171
AsuC2I CCSGG 8 cut(s) 50, 300, 360, 361, 448, 1553, 2092, 2605
AsuHPI GGTGA 6 cut(s) 163, 569, 958, 967, 1449, 2663
AsuII TTCGAA 4 cut(s) 193, 413, 1034, 2098
AsuNHI GCTAGC 1 cut(s) 2741
AvaI CYCGRG 2 cut(s) 359, 1189
AvaII GGWCC 8 cut(s) 303, 356, 450, 549, 825, 1437, 1650, 1860
BalI TGGCCA 2 cut(s) 1908, 2338
BanI GGYRCC 2 cut(s) 719, 2342
BauI CACGAG 2 cut(s) 1286, 2294
BbsI GAAGAC 5 cut(s) 763, 970, 2217, 2574, 2691
BbvI GCAGC 6 cut(s) 241, 269, 397, 1873, 2177, 2336
BceAI ACGGC 3 cut(s) 58, 1091, 1823
BciT130I CCWGG 3 cut(s) 1648, 1910, 2340
BciVI GTATCC 1 cut(s) 286
BclI TGATCA 4 cut(s) 490, 804, 850, 1471
BcnI CCSGG 8 cut(s) 50, 300, 360, 361, 448, 1553, 2092, 2605
BcoDI GTCTC 2 cut(s) 1706, 2374
BfaI CTAG 3 cut(s) 1533, 2205, 2742
BfmI CTRYAG 2 cut(s) 255, 2411
BfoI RGCGCY 1 cut(s) 216
BfuAI ACCTGC 1 cut(s) 2444
BfuI GTATCC 1 cut(s) 286
BisI GCNGC 9 cut(s) 255, 258, 386, 654, 1006, 1887, 2166, 2325, 2417
BlsI GCNGC 9 cut(s) 256, 259, 387, 655, 1007, 1888, 2167, 2326, 2418
Bme18I GGWCC 8 cut(s) 303, 356, 450, 549, 825, 1437, 1650, 1860
BmeT110I CYCGRG 2 cut(s) 359, 1189
BmiI GGNNCC 7 cut(s) 304, 358, 507, 721, 826, 1438, 2344
BmrI ACTGGG 1 cut(s) 46
BmtI GCTAGC 1 cut(s) 2745
BmuI ACTGGG 1 cut(s) 46
BpiI GAAGAC 5 cut(s) 763, 970, 2217, 2574, 2691
BpmI CTGGAG 1 cut(s) 1884
Bpu10I CCTNAGC 1 cut(s) 510
Bpu14I TTCGAA 4 cut(s) 193, 413, 1034, 2098
BpuEI CTTGAG 2 cut(s) 140, 2427
BpuMI CCSGG 8 cut(s) 50, 300, 360, 361, 448, 1553, 2092, 2605
Bsa29I ATCGAT 2 cut(s) 204, 1463
BsaAI YACGTR 1 cut(s) 1561
BsaBI GATNNNNATC 1 cut(s) 763
BsaHI GRCGYC 2 cut(s) 432, 1160
BsaI GGTCTC 1 cut(s) 1706
BsaJI CCNNGG 7 cut(s) 299, 352, 359, 446, 552, 2259, 2333
BsaWI WCCGGW 2 cut(s) 749, 783
BsaXI ACNNNNNCTCC 2 cut(s) 2466, 2496
Bsc4I CCNNNNNNNGG 4 cut(s) 1209, 2339, 2423, 2643
Bse118I RCCGGY 4 cut(s) 749, 783, 940, 1165
Bse1I ACTGG 2 cut(s) 41, 1862
Bse3DI GCAATG 2 cut(s) 1256, 2434
Bse8I GATNNNNATC 1 cut(s) 763
BseBI CCWGG 3 cut(s) 1648, 1910, 2340
BseCI ATCGAT 2 cut(s) 204, 1463
BseDI CCNNGG 7 cut(s) 299, 352, 359, 446, 552, 2259, 2333
BseJI GATNNNNATC 1 cut(s) 763
BseLI CCNNNNNNNGG 4 cut(s) 1209, 2339, 2423, 2643
BseMI GCAATG 2 cut(s) 1256, 2434
BseMII CTCAG 5 cut(s) 501, 889, 1034, 1218, 2268
BseNI ACTGG 2 cut(s) 41, 1862
BseRI GAGGAG 4 cut(s) 611, 648, 1536, 2547
BseXI GCAGC 6 cut(s) 241, 269, 397, 1873, 2177, 2336
BsgI GTGCAG 2 cut(s) 1362, 1977
Bsh1236I CGCG 2 cut(s) 93, 2836
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 5 cut(s) 45, 1489, 1908, 2338, 2865
BshNI GGYRCC 2 cut(s) 719, 2342
BshTI ACCGGT 2 cut(s) 749, 783
BshVI ATCGAT 2 cut(s) 204, 1463
BsiEI CGRYCG 1 cut(s) 70
BsiHKCI CYCGRG 2 cut(s) 359, 1189
BslFI GGGAC 4 cut(s) 53, 65, 838, 1114
BslI CCNNNNNNNGG 4 cut(s) 1209, 2339, 2423, 2643
BsmAI GTCTC 2 cut(s) 1706, 2374
BsmFI GGGAC 4 cut(s) 53, 65, 838, 1114
BsmI GAATGC 2 cut(s) 794, 2475
BsnI GGCC 5 cut(s) 45, 1489, 1908, 2338, 2865
Bso31I GGTCTC 1 cut(s) 1706
BsoBI CYCGRG 2 cut(s) 359, 1189
Bsp119I TTCGAA 4 cut(s) 193, 413, 1034, 2098
Bsp1407I TGTACA 1 cut(s) 475
Bsp19I CCATGG 1 cut(s) 2333
BspANI GGCC 5 cut(s) 45, 1489, 1908, 2338, 2865
BspCNI CTCAG 5 cut(s) 502, 888, 1035, 1217, 2267
BspDI ATCGAT 2 cut(s) 204, 1463
BspFNI CGCG 2 cut(s) 93, 2836
BspHI TCATGA 1 cut(s) 1870
BspLI GGNNCC 7 cut(s) 304, 358, 507, 721, 826, 1438, 2344
BspMAI CTGCAG 1 cut(s) 259
BspMI ACCTGC 1 cut(s) 2444
BspOI GCTAGC 1 cut(s) 2745
BspPI GGATC 4 cut(s) 706, 727, 1359, 2570
BspQI GCTCTTC 1 cut(s) 1031
BspT104I TTCGAA 4 cut(s) 193, 413, 1034, 2098
BspT107I GGYRCC 2 cut(s) 719, 2342
BspTNI GGTCTC 1 cut(s) 1706
BsrBI CCGCTC 2 cut(s) 13, 656
BsrDI GCAATG 2 cut(s) 1256, 2434
BsrFI RCCGGY 4 cut(s) 749, 783, 940, 1165
BsrGI TGTACA 1 cut(s) 475
BsrI ACTGG 2 cut(s) 41, 1862
BssAI RCCGGY 4 cut(s) 749, 783, 940, 1165
BssECI CCNNGG 7 cut(s) 299, 352, 359, 446, 552, 2259, 2333
BssNAI GTATAC 2 cut(s) 681, 1088
BssNI GRCGYC 2 cut(s) 432, 1160
BssSI CACGAG 2 cut(s) 1286, 2294
BssT1I CCWWGG 2 cut(s) 352, 2333
Bst1107I GTATAC 2 cut(s) 681, 1088
Bst2BI CACGAG 2 cut(s) 1286, 2294
Bst2UI CCWGG 3 cut(s) 1648, 1910, 2340
Bst4CI ACNGT 6 cut(s) 158, 499, 1155, 1693, 1767, 1943
Bst6I CTCTTC 4 cut(s) 11, 103, 1031, 1054
BstACI GRCGYC 2 cut(s) 432, 1160
BstAPI GCANNNNNTGC 1 cut(s) 1016
BstAUI TGTACA 1 cut(s) 475
BstBAI YACGTR 1 cut(s) 1561
BstBI TTCGAA 4 cut(s) 193, 413, 1034, 2098
BstC8I GCNNGC 2 cut(s) 1179, 2743
BstDEI CTNAG 5 cut(s) 510, 875, 1043, 1204, 2254
BstDSI CCRYGG 2 cut(s) 2259, 2333
BstENI CCTNNNNNAGG 1 cut(s) 2641
BstFNI CGCG 2 cut(s) 93, 2836
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 4 cut(s) 95, 215, 1010, 1171
BstMAI GTCTC 2 cut(s) 1706, 2374
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 9 cut(s) 402, 431, 1016, 1168, 2047, 2416, 2678, 2742, 2842
BstNI CCWGG 3 cut(s) 1648, 1910, 2340
BstNSI RCATGY 2 cut(s) 166, 1568
BstSFI CTRYAG 2 cut(s) 255, 2411
BstUI CGCG 2 cut(s) 93, 2836
BstV1I GCAGC 6 cut(s) 241, 269, 397, 1873, 2177, 2336
BstV2I GAAGAC 5 cut(s) 763, 970, 2217, 2574, 2691
BstX2I RGATCY 1 cut(s) 2575
BstXI CCANNNNNNTGG 1 cut(s) 716
BstYI RGATCY 1 cut(s) 2575
BstZ17I GTATAC 2 cut(s) 681, 1088
Bsu15I ATCGAT 2 cut(s) 204, 1463
BsuI GTATCC 1 cut(s) 286
BsuRI GGCC 5 cut(s) 45, 1489, 1908, 2338, 2865
BsuTUI ATCGAT 2 cut(s) 204, 1463
BtgI CCRYGG 2 cut(s) 2259, 2333
BtsI GCAGTG 2 cut(s) 249, 1881
BtsIMutI CAGTG 6 cut(s) 249, 309, 1021, 1804, 1881, 2312
BveI ACCTGC 1 cut(s) 2444
Cac8I GCNNGC 2 cut(s) 1179, 2743
CciI TCATGA 1 cut(s) 1870
CfoI GCGC 4 cut(s) 95, 215, 1010, 1171
Cfr10I RCCGGY 4 cut(s) 749, 783, 940, 1165
Cfr9I CCCGGG 1 cut(s) 359
ClaI ATCGAT 2 cut(s) 204, 1463
CseI GACGC 3 cut(s) 440, 916, 1168
Csp6I GTAC 6 cut(s) 476, 482, 934, 1558, 1568, 2283
CspAI ACCGGT 2 cut(s) 749, 783
CviQI GTAC 6 cut(s) 476, 482, 934, 1558, 1568, 2283
DdeI CTNAG 5 cut(s) 510, 875, 1043, 1204, 2254
EaeI YGGCCR 2 cut(s) 1906, 2336
Eam1104I CTCTTC 4 cut(s) 11, 103, 1031, 1054
EarI CTCTTC 4 cut(s) 11, 103, 1031, 1054
EciI GGCGGA 1 cut(s) 2550
Eco130I CCWWGG 2 cut(s) 352, 2333
Eco31I GGTCTC 1 cut(s) 1706
Eco47I GGWCC 8 cut(s) 303, 356, 450, 549, 825, 1437, 1650, 1860
Eco88I CYCGRG 2 cut(s) 359, 1189
EcoNI CCTNNNNNAGG 1 cut(s) 2641
EcoO109I RGGNCCY 3 cut(s) 356, 1437, 2864
EcoRI GAATTC 1 cut(s) 1408
EcoRII CCWGG 3 cut(s) 1646, 1908, 2338
EcoT14I CCWWGG 2 cut(s) 352, 2333
EcoT22I ATGCAT 2 cut(s) 2434, 2536
ErhI CCWWGG 2 cut(s) 352, 2333
FalI AAGNNNNNCTT 4 cut(s) 1467, 1499, 1709, 1741
FaqI GGGAC 4 cut(s) 53, 65, 838, 1114
FauI CCCGC 2 cut(s) 1186, 1425
FauNDI CATATG 1 cut(s) 1947
FbaI TGATCA 4 cut(s) 490, 804, 850, 1471
FblI GTMKAC 3 cut(s) 579, 680, 1087
Fnu4HI GCNGC 9 cut(s) 255, 258, 386, 654, 1006, 1887, 2166, 2325, 2417
Fsp4HI GCNGC 9 cut(s) 255, 258, 386, 654, 1006, 1887, 2166, 2325, 2417
FspBI CTAG 3 cut(s) 1533, 2205, 2742
GlaI GCGC 4 cut(s) 94, 214, 1009, 1170
GluI GCNGC 9 cut(s) 255, 258, 386, 654, 1006, 1887, 2166, 2325, 2417
GsuI CTGGAG 1 cut(s) 1884
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 5 cut(s) 45, 1489, 1908, 2338, 2865
HgaI GACGC 3 cut(s) 440, 916, 1168
HhaI GCGC 4 cut(s) 95, 215, 1010, 1171
Hin1I GRCGYC 2 cut(s) 432, 1160
Hin6I GCGC 4 cut(s) 93, 213, 1008, 1169
HinP1I GCGC 4 cut(s) 93, 213, 1008, 1169
HincII GTYRAC 3 cut(s) 27, 580, 1158
HindII GTYRAC 3 cut(s) 27, 580, 1158
HindIII AAGCTT 1 cut(s) 567
HphI GGTGA 6 cut(s) 163, 569, 958, 967, 1449, 2663
Hpy166II GTNNAC 8 cut(s) 27, 580, 681, 841, 1088, 1158, 1457, 1855
Hpy8I GTNNAC 8 cut(s) 27, 580, 681, 841, 1088, 1158, 1457, 1855
Hpy99I CGWCG 6 cut(s) 59, 62, 65, 68, 238, 932
HpyAV CCTTC 8 cut(s) 226, 977, 1507, 1610, 1903, 2559, 2643, 2792
HpyCH4III ACNGT 6 cut(s) 158, 499, 1155, 1693, 1767, 1943
HpyCH4IV ACGT 3 cut(s) 23, 1560, 2118
HpyF10VI GCNNNNNNNGC 9 cut(s) 402, 431, 1016, 1168, 2047, 2416, 2678, 2742, 2842
HpyF3I CTNAG 5 cut(s) 510, 875, 1043, 1204, 2254
HpySE526I ACGT 3 cut(s) 23, 1560, 2118
Hsp92I GRCGYC 2 cut(s) 432, 1160
HspAI GCGC 4 cut(s) 93, 213, 1008, 1169
Ksp22I TGATCA 4 cut(s) 490, 804, 850, 1471
LguI GCTCTTC 1 cut(s) 1031
LmnI GCTCC 6 cut(s) 10, 265, 505, 647, 661, 1246
Lsp1109I GCAGC 6 cut(s) 241, 269, 397, 1873, 2177, 2336
MaeI CTAG 3 cut(s) 1533, 2205, 2742
MaeII ACGT 3 cut(s) 23, 1560, 2118
MaeIII GTNAC 7 cut(s) 522, 557, 1753, 1879, 2074, 2119, 2782
MbiI CCGCTC 2 cut(s) 13, 656
MflI RGATCY 1 cut(s) 2575
MlsI TGGCCA 2 cut(s) 1908, 2338
MluNI TGGCCA 2 cut(s) 1908, 2338
MlyI GAGTC 5 cut(s) 89, 867, 2116, 2207, 2624
Mox20I TGGCCA 2 cut(s) 1908, 2338
Mph1103I ATGCAT 2 cut(s) 2434, 2536
MscI TGGCCA 2 cut(s) 1908, 2338
MseI TTAA 8 cut(s) 381, 399, 902, 1308, 1727, 1973, 2622, 2872
MslI CAYNNNNRTG 5 cut(s) 287, 728, 1626, 2117, 2223
Msp20I TGGCCA 2 cut(s) 1908, 2338
Mva1269I GAATGC 2 cut(s) 794, 2475
MvaI CCWGG 3 cut(s) 1648, 1910, 2340
MvnI CGCG 2 cut(s) 93, 2836
MwoI GCNNNNNNNGC 9 cut(s) 402, 431, 1016, 1168, 2047, 2416, 2678, 2742, 2842
NciI CCSGG 8 cut(s) 50, 300, 360, 361, 448, 1553, 2092, 2605
NcoI CCATGG 1 cut(s) 2333
NdeI CATATG 1 cut(s) 1947
NheI GCTAGC 1 cut(s) 2741
NlaIV GGNNCC 7 cut(s) 304, 358, 507, 721, 826, 1438, 2344
NmuCI GTSAC 5 cut(s) 557, 1753, 1879, 2119, 2782
NsiI ATGCAT 2 cut(s) 2434, 2536
NspI RCATGY 2 cut(s) 166, 1568
NspV TTCGAA 4 cut(s) 193, 413, 1034, 2098
OliI CACNNNNGTG 2 cut(s) 728, 2117
PaeR7I CTCGAG 1 cut(s) 1189
PagI TCATGA 1 cut(s) 1870
PaqCI CACCTGC 1 cut(s) 2444
PciSI GCTCTTC 1 cut(s) 1031
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 63
PctI GAATGC 2 cut(s) 794, 2475
PflFI GACNNNGTC 1 cut(s) 371
PfoI TCCNGGA 4 cut(s) 48, 1551, 2090, 2603
PinAI ACCGGT 2 cut(s) 749, 783
PkrI GCNGC 9 cut(s) 256, 259, 387, 655, 1007, 1888, 2167, 2326, 2418
PleI GAGTC 5 cut(s) 88, 867, 2116, 2207, 2623
PpsI GAGTC 5 cut(s) 88, 867, 2116, 2207, 2623
Ppu21I YACGTR 1 cut(s) 1561
PpuMI RGGWCCY 2 cut(s) 356, 1437
Psp5II RGGWCCY 2 cut(s) 356, 1437
Psp6I CCWGG 3 cut(s) 1646, 1908, 2338
PspGI CCWGG 3 cut(s) 1646, 1908, 2338
PspN4I GGNNCC 7 cut(s) 304, 358, 507, 721, 826, 1438, 2344
PspPPI RGGWCCY 2 cut(s) 356, 1437
PspXI VCTCGAGB 1 cut(s) 1189
PstI CTGCAG 1 cut(s) 259
PsuI RGATCY 1 cut(s) 2575
PsyI GACNNNGTC 1 cut(s) 371
RsaI GTAC 6 cut(s) 477, 483, 935, 1559, 1569, 2284
RsaNI GTAC 6 cut(s) 476, 482, 934, 1558, 1568, 2283
RseI CAYNNNNRTG 5 cut(s) 287, 728, 1626, 2117, 2223
SalI GTCGAC 1 cut(s) 578
SapI GCTCTTC 1 cut(s) 1031
SaqAI TTAA 8 cut(s) 381, 399, 902, 1308, 1727, 1973, 2622, 2872
SatI GCNGC 9 cut(s) 255, 258, 386, 654, 1006, 1887, 2166, 2325, 2417
SchI GAGTC 5 cut(s) 89, 867, 2116, 2207, 2624
SfcI CTRYAG 2 cut(s) 255, 2411
Sfr274I CTCGAG 1 cut(s) 1189
SfuI TTCGAA 4 cut(s) 193, 413, 1034, 2098
SgrAI CRCCGGYG 2 cut(s) 940, 1165
SinI GGWCC 8 cut(s) 303, 356, 450, 549, 825, 1437, 1650, 1860
SlaI CTCGAG 1 cut(s) 1189
SmaI CCCGGG 1 cut(s) 361
SmiMI CAYNNNNRTG 5 cut(s) 287, 728, 1626, 2117, 2223
SmlI CTYRAG 3 cut(s) 119, 1189, 2442
SmoI CTYRAG 3 cut(s) 119, 1189, 2442
SspI AATATT 1 cut(s) 2854
SspMI CTAG 3 cut(s) 1533, 2205, 2742
StyI CCWWGG 2 cut(s) 352, 2333
TaaI ACNGT 6 cut(s) 158, 499, 1155, 1693, 1767, 1943
TaiI ACGT 3 cut(s) 26, 1563, 2121
TaqII GACCGA 4 cut(s) 679, 757, 842, 1877
TatI WGTACW 1 cut(s) 475
TauI GCSGC 3 cut(s) 656, 1008, 2419
Tru1I TTAA 8 cut(s) 381, 399, 902, 1308, 1727, 1973, 2622, 2872
Tru9I TTAA 8 cut(s) 381, 399, 902, 1308, 1727, 1973, 2622, 2872
TscAI CASTG 6 cut(s) 256, 316, 1021, 1804, 1888, 2312
TseFI GTSAC 5 cut(s) 557, 1753, 1879, 2119, 2782
TseI GCWGC 6 cut(s) 254, 257, 385, 1886, 2165, 2324
Tsp45I GTSAC 5 cut(s) 557, 1753, 1879, 2119, 2782
TspGWI ACGGA 4 cut(s) 91, 666, 2199, 2362
TspMI CCCGGG 1 cut(s) 359
TspRI CASTG 6 cut(s) 256, 316, 1021, 1804, 1888, 2312
Tth111I GACNNNGTC 1 cut(s) 371
VpaK11BI GGWCC 8 cut(s) 303, 356, 450, 549, 825, 1437, 1650, 1860
XagI CCTNNNNNAGG 1 cut(s) 2641
XapI RAATTY 5 cut(s) 914, 1021, 1408, 2100, 2821
XceI RCATGY 2 cut(s) 166, 1568
XhoI CTCGAG 1 cut(s) 1189
XmaI CCCGGG 1 cut(s) 359
XmiI GTMKAC 3 cut(s) 579, 680, 1087
XspI CTAG 3 cut(s) 1533, 2205, 2742
Zsp2I ATGCAT 2 cut(s) 2434, 2536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.