Rroxscaffold_6G00414170

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0012301
Physical Location & Seq
Reverse (-)
36457338 .. 36460994
3657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414170.1

Sequence Viewer

Length: 2793 bp
ATGATCATGGAGCCGAAGATGGAGCGGAAGAGGACCTTCGCTATGAACTGGGACGGCCTCCACGACGAAGAAGACGACGACCGCTTCTTCGAGTCCCGGGAGAGAATGTCCTCCGCCGTGCCTATCGACCTCGAAGCCTCCTCCTCCGACGATGAGGACTTCGACGACAGTCGCATGTCATTCGCCTCCTGCGTCTCCTCCGTCCGAAACGACGTCGAGGTGCTGCGGGCCTTGTCCGCCGCCGCCGCCGAGCCCTCCTTCGCCTCGTCGCCTCCGATGTCGCCGGACTACAACATCTGGATGGCCGCGCCGGGGTCGATCTCCGAACGCCGCAGGCGGTTGTTGCAGGGCATGGGATTGGCCAACGGCGGCAAGGACATCGTCAGCTTCAGACGCCTCGTTTCGAATAAAGCCCCAAACGGCGTCGTTCCGGCCGTTACGAGTTGCCCTACTCGCTCTCGGGAAAAGATACAAGTTCCCGAAACGGCGCCCGAACCCGATCCCGATCGGGAGCCGGAGCCGGGGGCTTCGAATTCACCGTTGCCGGTGATGCTTGTACGGTCCAGATCGGAAGGAGAGATCGAAGCTTTCTCGGCCGATAAACGAAGGAAAGAGGAGGTAATTGGCACCGTCTCGAAACAACGGCTGACAAGAACGTCGTCGGCGATCGCGGCTCCTAGCTCTAAGATGTGTTTTTCCACGACGCCTCAAAGCGACGTAGGAACCAAGACCGTCCGATCTAGGCAAAACAGCGCAGCATTGTCGACGGAGTTCACCAACAATTTAGGGGCGTTCTTCTTGATTAAAAATCTGGACACGGGAACCGAGTTCATTGTGAACGAGTACGATCAAAACGGGTGTTGGAACAAGTTTAGTGATCTCCAAACGGGGAAGAAATTGACCATGGACGAGTTTGAGAAGTCTGTCGGGTACTCGCCGGTGGTGAAGGAGGTGATGCGGCGGCAAAATGTCAACATTGAAGCCGGGATTGAGAGGAAGGTACCGGCAGGAGCCAATTCGTTTGTGTCAAAGAGTTTGAGGATGAGCAAGAGAAAGGTGGCTCTCTTGAAGAATAGCATAAAAGGTGTGGGAAGTGCTGTGAGTGTACTAATTGGGGATAAGGATCAACACGCCTCGTCACCGCCGCCGCCGCTGCCGCAGGACCAGAAATCAAGCAAGGCTAATTCGGGGTCTGGTTCGGAGTGGATCAAAGTTAGGCAGACTGGGAAGTCATATAAGGAGCTATCAGCATTGCATTTGTGCCAGGAGATTCAGGCTCATGAGGGCTCAATTTGGACCATCAAGTTTAGCTTGGATGCTAGGTTTCTTGCTAGTGCTGGAGAGGACAGGGTCATTCATGTGTGGGAAGTTCAAGAATGTGAGATTATGTCCATGGATAGCAATGCGAACCCGCTGCATCCCTCCTCGGTGTCCCCATATCCGGATCGATCAATGTGTCTTAGCTCTGAAGCTTCAATTTCCTCTGAGAAGAAGAAGAAGGGGAAGGGAGGCTCTTCTAGGAAAACTAATCCCATTCCTGACTACGTCCATGTGCCCGAATTCGTGTTTGCCCTCTCGGAGAAACCCGTGTGCTCTTTTGAAGGTCATTTGGATGCAGTACTGGACCTGTCATGGTCCAGAGATCAGCTATTGCTTTCATCTTCAATGGACAAAACTGTTAGATTATGGGATATGGTGACCAAGAGTTGTTTAAAGATGTTTGCCCACAATGATTATGTAACTTGCATACAGTTCAATCCATTGGACGACAGTGTTTTTCTCAGCGGCTCCCTTGATGCGAAGCTTCGATTATGGGACATACCTGATCGAAGAGTGGTGGACTGGACTGATCTTCATGAAATGGTCACCGCTGCATGCTATACTCCAGATGGCCAGGCTGCCATCATTGGGTCGCACAAAGGGAACTGTCGTATGTACAATGCAGAAGATTGTAAATTAAGTCAAGAAGGCCAGATTGATATTAAAAACAAGAAGAAGAATCAAGCAAAGAAAATTACCGGTTTCCAGTTTTCTCCGATTAATCCATCTGAAATGCTTGTCACATCTGCTGATTCCCGGATTAGGATTGTGGACGGTAAAGATGTCACTCATAAGTTTAGAGGTTTCCGAAATACAAGCAGTCAAATTGGAGCGTCATTCACTCCCAATGGAAAGTACATTATATGTGCCAGTGAAGATTCTCATGTTTATTGTAAAGATGTTTCAGTAGCAATCCCATGGCCTGGCACCATAAAAGGTGACCCACCCCCGCCTCTGTCTGTGCATTCTTCTAAGAGGCACTCAAAACGTTCCTCTGTACAGCCAGTTTCAGGAACTGCATCACCTACTCAAGACGGAGGAGACAGCAAGAGATTCCTGCCACCTCTTCCAAAGAAAAACACTAATCCTGGTACTCCTCCTAGTGCGACGGCGGAAAAAAGCACTAATCCTACTACTCCTCCTAATGCGACAGCGGAGAAACCTTCAACTCCCCCGCCGGAAGATCAGGATCCATCTGAAATTTCTCGAACGGATTCTGGGATTGGCGAGTCATTCAGCTCGGACCCTTCCTCTATTAGGTATGGTGGCGATTCACCTTCTATATCTTCTGGCAATGCATCATCATCTTGGTCTTCCTCTTGGTCCTCTTTGTTTGGGCATGAATCGAGCCAAACAACCCAAGCTTGGGGCTTGGTCATTGTGACAGCCACTCTGGAAGGTGAAATCAGGGCATATCAAAATTTTGGGATACCAAGAAGGGTGCCCGGCAGTTTTTTTGGAGGTCCAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

930

Amino Acids

101.67

Weight (kDa)

6.98

Isoelectric Point (pI)

55.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 416 - 463 4.5e-07 THOC3 beta-propeller domain
WD40 PF00400 421 - 455 7.5e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 526 - 649 5e-10 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 527 - 647 1.2e-16 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 527 - 650 5e-14 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 527 - 590 1.8e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 528 - 741 2.6e-35 WDR5 beta-propeller domain
WD40 PF00400 528 - 564 6.7e-08 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 529 - 639 5e-11 CDC20/Fizzy WD40 domain
EIF3I PF24805 530 - 729 3.8e-10 EIF3I
Beta-prop_THOC3 PF25174 531 - 573 1.6e-06 THOC3 beta-propeller domain
Beta-prop_IFT122_1st PF23381 533 - 647 6.4e-07 IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 533 - 613 2.9e-10 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 536 - 655 1.2e-07 WDR55
Beta-prop_THOC3 PF25174 536 - 737 1.3e-24 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 538 - 653 1.2e-09 Aladin seven-bladed propeller
WD40_Gbeta PF25391 547 - 648 4e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 551 - 691 1e-07 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 579 - 741 2.4e-09 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 591 - 741 1.4e-13 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37670 AT2G37670 AT5G02430
fragaria_vesca FvH4_6g13960
malus_domestica MD04G1127300.v1.1 MD12G1140500.v1.1
prunus_persica Prupe.6G253900_v2.0.a1 Prupe.6G253900_v2.0.a1
pyrus_communis pycom04g11410
rosa_chinensis RchiOBHm_Chr3g0466781
rosa_laevigata RLG00000024553
rosa_multiflora Rmu_sc0008017.1_g000009
rosa_roxburghii Rroxscaffold_6G00414170
rosa_rugosa Rorug03G0083800
rosa_samantha Rh3AG131000 Rh3BG152000 Rh3CG151900 Rh3DG152700
rosa_wichuraiana Rw3G012380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 655, 1228
AatII GACGTC 1 cut(s) 216
Acc65I GGTACC 1 cut(s) 1000
AccB1I GGYRCC 5 cut(s) 487, 626, 1000, 2246, 2761
AccB7I CCANNNNNTGG 1 cut(s) 2243
AccBSI CCGCTC 1 cut(s) 25
AccI GTMKAC 1 cut(s) 764
AccII CGCG 2 cut(s) 308, 671
AccIII TCCGGA 1 cut(s) 1441
AclI AACGTT 1 cut(s) 2308
AclWI GGATC 6 cut(s) 494, 1131, 1214, 1452, 2504, 2517
AcoI YGGCCR 5 cut(s) 303, 360, 432, 594, 1891
AcsI RAATTY 4 cut(s) 532, 1559, 2520, 2740
AcuI CTGAAG 2 cut(s) 373, 1488
AcyI GRCGYC 5 cut(s) 213, 394, 423, 488, 704
AgeI ACCGGT 1 cut(s) 2018
AgsI TTSAA 8 cut(s) 980, 1069, 1373, 1476, 1601, 1665, 1756, 2487
AjnI CCWGG 4 cut(s) 1263, 1893, 2242, 2407
Alw21I GWGCWC 1 cut(s) 1595
Alw26I GTCTC 3 cut(s) 199, 637, 2355
AlwI GGATC 6 cut(s) 494, 1131, 1214, 1452, 2504, 2517
AlwNI CAGNNNCTG 1 cut(s) 2336
Ama87I CYCGRG 2 cut(s) 96, 459
Aor13HI TCCGGA 1 cut(s) 1441
AoxI GGCC 9 cut(s) 55, 228, 303, 360, 432, 594, 1891, 1969, 2240
ApeKI GCWGC 6 cut(s) 223, 755, 1153, 1414, 1871, 1898
ApoI RAATTY 4 cut(s) 532, 1559, 2520, 2740
ArsI GACNNNNNNTTYG 2 cut(s) 71, 103
AseI ATTAAT 1 cut(s) 2040
AsiGI ACCGGT 1 cut(s) 2018
AsiSI GCGATCGC 1 cut(s) 669
Asp700I GAANNNNTTC 1 cut(s) 2533
Asp718I GGTACC 1 cut(s) 1000
AspLEI GCGC 3 cut(s) 310, 490, 755
AsuC2I CCSGG 7 cut(s) 97, 98, 312, 522, 985, 2077, 2766
AsuII TTCGAA 2 cut(s) 404, 530
AvaI CYCGRG 2 cut(s) 96, 459
AvaII GGWCC 9 cut(s) 33, 561, 1162, 1296, 1624, 1635, 2563, 2643, 2783
BaeGI GKGCMC 2 cut(s) 1557, 2766
BalI TGGCCA 2 cut(s) 362, 1893
BamHI GGATCC 1 cut(s) 2509
BanI GGYRCC 5 cut(s) 487, 626, 1000, 2246, 2761
BanII GRGCYC 2 cut(s) 255, 1289
BbsI GAAGAC 2 cut(s) 78, 2625
Bbv12I GWGCWC 1 cut(s) 1595
BbvI GCAGC 6 cut(s) 210, 767, 1140, 1401, 1858, 1885
BccI CCATC 7 cut(s) 13, 295, 1307, 1883, 1910, 2053, 2521
BceAI ACGGC 8 cut(s) 70, 101, 382, 419, 436, 501, 659, 2445
BcgI CGANNNNNNTGC 8 cut(s) 163, 197, 361, 395, 744, 778, 1396, 1430
BciT130I CCWGG 4 cut(s) 1265, 1895, 2244, 2409
BciVI GTATCC 1 cut(s) 2742
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 7 cut(s) 97, 98, 312, 522, 985, 2077, 2766
BcoDI GTCTC 3 cut(s) 199, 637, 2355
BfaI CTAG 7 cut(s) 678, 741, 1320, 1332, 1518, 2421, 2791
BfoI RGCGCY 1 cut(s) 491
BfuI GTATCC 1 cut(s) 2742
BmcAI AGTACT 1 cut(s) 1620
Bme18I GGWCC 9 cut(s) 33, 561, 1162, 1296, 1624, 1635, 2563, 2643, 2783
BmeT110I CYCGRG 2 cut(s) 96, 459
BmrI ACTGGG 2 cut(s) 58, 1233
BmsI GCATC 8 cut(s) 540, 945, 1306, 1426, 1603, 1786, 2348, 2627
BmuI ACTGGG 2 cut(s) 58, 1233
BoxI GACNNNNGTC 1 cut(s) 168
BpiI GAAGAC 2 cut(s) 78, 2625
BpmI CTGGAG 2 cut(s) 1359, 1869
Bpu14I TTCGAA 2 cut(s) 404, 530
BpuEI CTTGAG 1 cut(s) 2334
BpuMI CCSGG 7 cut(s) 97, 98, 312, 522, 985, 2077, 2766
Bsa29I ATCGAT 1 cut(s) 1447
BsaBI GATNNNNATC 3 cut(s) 504, 1122, 2508
BsaHI GRCGYC 5 cut(s) 213, 394, 423, 488, 704
BsaJI CCNNGG 7 cut(s) 96, 311, 521, 903, 1392, 1425, 2237
BsaWI WCCGGW 2 cut(s) 1441, 2018
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 2443, 2473
Bse118I RCCGGY 4 cut(s) 544, 937, 1003, 2018
Bse1I ACTGG 7 cut(s) 53, 1228, 1626, 1847, 2026, 2190, 2324
Bse3DI GCAATG 3 cut(s) 1250, 1408, 2620
Bse8I GATNNNNATC 3 cut(s) 504, 1122, 2508
BseAI TCCGGA 1 cut(s) 1441
BseBI CCWGG 4 cut(s) 1265, 1895, 2244, 2409
BseCI ATCGAT 1 cut(s) 1447
BseDI CCNNGG 7 cut(s) 96, 311, 521, 903, 1392, 1425, 2237
BseGI GGATG 5 cut(s) 306, 1047, 1321, 1417, 1618
BseJI GATNNNNATC 3 cut(s) 504, 1122, 2508
BseMI GCAATG 3 cut(s) 1250, 1408, 2620
BseMII CTCAG 2 cut(s) 1476, 1795
BseNI ACTGG 7 cut(s) 53, 1228, 1626, 1847, 2026, 2190, 2324
BseRI GAGGAG 8 cut(s) 130, 133, 187, 629, 1414, 2373, 2406, 2448
BseSI GKGCMC 2 cut(s) 1557, 2766
BseX3I CGGCCG 2 cut(s) 432, 594
BseXI GCAGC 6 cut(s) 210, 767, 1140, 1401, 1858, 1885
Bsh1236I CGCG 2 cut(s) 308, 671
Bsh1285I CGRYCG 5 cut(s) 82, 435, 508, 597, 669
BshFI GGCC 9 cut(s) 57, 230, 305, 362, 434, 596, 1893, 1971, 2242
BshNI GGYRCC 5 cut(s) 487, 626, 1000, 2246, 2761
BshTI ACCGGT 1 cut(s) 2018
BshVI ATCGAT 1 cut(s) 1447
BsiEI CGRYCG 5 cut(s) 82, 435, 508, 597, 669
BsiHKAI GWGCWC 1 cut(s) 1595
BsiHKCI CYCGRG 2 cut(s) 96, 459
BslFI GGGAC 4 cut(s) 65, 79, 1417, 1829
BsmAI GTCTC 3 cut(s) 199, 637, 2355
BsmBI CGTCTC 2 cut(s) 199, 637
BsmFI GGGAC 4 cut(s) 65, 79, 1417, 1829
BsmI GAATGC 1 cut(s) 2284
BsnI GGCC 9 cut(s) 57, 230, 305, 362, 434, 596, 1893, 1971, 2242
BsoBI CYCGRG 2 cut(s) 96, 459
Bsp119I TTCGAA 2 cut(s) 404, 530
Bsp1286I GDGCHC 5 cut(s) 255, 1289, 1557, 1595, 2766
Bsp13I TCCGGA 1 cut(s) 1441
Bsp1407I TGTACA 2 cut(s) 1935, 2317
Bsp19I CCATGG 3 cut(s) 903, 1392, 2237
BspANI GGCC 9 cut(s) 57, 230, 305, 362, 434, 596, 1893, 1971, 2242
BspCNI CTCAG 2 cut(s) 1477, 1794
BspDI ATCGAT 1 cut(s) 1447
BspEI TCCGGA 1 cut(s) 1441
BspFNI CGCG 2 cut(s) 308, 671
BspHI TCATGA 2 cut(s) 1279, 1855
BspPI GGATC 6 cut(s) 494, 1131, 1214, 1452, 2504, 2517
BspQI GCTCTTC 1 cut(s) 1519
BspT104I TTCGAA 2 cut(s) 404, 530
BspT107I GGYRCC 5 cut(s) 487, 626, 1000, 2246, 2761
BsrBI CCGCTC 1 cut(s) 25
BsrDI GCAATG 3 cut(s) 1250, 1408, 2620
BsrFI RCCGGY 4 cut(s) 544, 937, 1003, 2018
BsrGI TGTACA 2 cut(s) 1935, 2317
BsrI ACTGG 7 cut(s) 53, 1228, 1626, 1847, 2026, 2190, 2324
BssAI RCCGGY 4 cut(s) 544, 937, 1003, 2018
BssECI CCNNGG 7 cut(s) 96, 311, 521, 903, 1392, 1425, 2237
BssNI GRCGYC 5 cut(s) 213, 394, 423, 488, 704
BssT1I CCWWGG 3 cut(s) 903, 1392, 2237
Bst2UI CCWGG 4 cut(s) 1265, 1895, 2244, 2409
Bst6I CTCTTC 4 cut(s) 23, 1519, 1825, 2391
BstACI GRCGYC 5 cut(s) 213, 394, 423, 488, 704
BstAUI TGTACA 2 cut(s) 1935, 2317
BstBI TTCGAA 2 cut(s) 404, 530
BstC8I GCNNGC 3 cut(s) 228, 335, 1876
BstDEI CTNAG 5 cut(s) 684, 1460, 1485, 1781, 2292
BstDSI CCRYGG 3 cut(s) 903, 1392, 2237
BstEII GGTNACC 3 cut(s) 1696, 1864, 2258
BstENI CCTNNNNNAGG 1 cut(s) 2575
BstF5I GGATG 5 cut(s) 306, 1047, 1321, 1417, 1618
BstFNI CGCG 2 cut(s) 308, 671
BstH2I RGCGCY 1 cut(s) 491
BstHHI GCGC 3 cut(s) 310, 490, 755
BstMAI GTCTC 3 cut(s) 199, 637, 2355
BstMCI CGRYCG 5 cut(s) 82, 435, 508, 597, 669
BstNI CCWGG 4 cut(s) 1265, 1895, 2244, 2409
BstNSI RCATGY 2 cut(s) 178, 1878
BstPAI GACNNNNGTC 1 cut(s) 168
BstPI GGTNACC 3 cut(s) 1696, 1864, 2258
BstSLI GKGCMC 2 cut(s) 1557, 2766
BstUI CGCG 2 cut(s) 308, 671
BstV1I GCAGC 6 cut(s) 210, 767, 1140, 1401, 1858, 1885
BstV2I GAAGAC 2 cut(s) 78, 2625
BstX2I RGATCY 1 cut(s) 2509
BstYI RGATCY 1 cut(s) 2509
BstZI CGGCCG 2 cut(s) 432, 594
Bsu15I ATCGAT 1 cut(s) 1447
BsuI GTATCC 1 cut(s) 2742
BsuRI GGCC 9 cut(s) 57, 230, 305, 362, 434, 596, 1893, 1971, 2242
BsuTUI ATCGAT 1 cut(s) 1447
BtgI CCRYGG 3 cut(s) 903, 1392, 2237
BtsCI GGATG 5 cut(s) 306, 1047, 1321, 1417, 1618
BtsIMutI CAGTG 2 cut(s) 1777, 2197
Cac8I GCNNGC 3 cut(s) 228, 335, 1876
CaiI CAGNNNCTG 1 cut(s) 2336
CciI TCATGA 2 cut(s) 1279, 1855
CfoI GCGC 3 cut(s) 310, 490, 755
Cfr10I RCCGGY 4 cut(s) 544, 937, 1003, 2018
Cfr9I CCCGGG 1 cut(s) 96
ClaI ATCGAT 1 cut(s) 1447
CseI GACGC 5 cut(s) 181, 402, 412, 712, 2142
CspAI ACCGGT 1 cut(s) 2018
DdeI CTNAG 5 cut(s) 684, 1460, 1485, 1781, 2292
DinI GGCGCC 1 cut(s) 489
DraI TTTAAA 1 cut(s) 1713
DrdI GACNNNNNNGTC 2 cut(s) 655, 1228
DseDI GACNNNNNNGTC 2 cut(s) 655, 1228
EaeI YGGCCR 5 cut(s) 303, 360, 432, 594, 1891
EagI CGGCCG 2 cut(s) 432, 594
Eam1104I CTCTTC 4 cut(s) 23, 1519, 1825, 2391
EarI CTCTTC 4 cut(s) 23, 1519, 1825, 2391
EciI GGCGGA 3 cut(s) 103, 226, 2447
EclXI CGGCCG 2 cut(s) 432, 594
Eco130I CCWWGG 3 cut(s) 903, 1392, 2237
Eco24I GRGCYC 2 cut(s) 255, 1289
Eco47I GGWCC 9 cut(s) 33, 561, 1162, 1296, 1624, 1635, 2563, 2643, 2783
Eco52I CGGCCG 2 cut(s) 432, 594
Eco57I CTGAAG 2 cut(s) 373, 1488
Eco88I CYCGRG 2 cut(s) 96, 459
Eco91I GGTNACC 3 cut(s) 1696, 1864, 2258
EcoNI CCTNNNNNAGG 1 cut(s) 2575
EcoO109I RGGNCCY 1 cut(s) 33
EcoO65I GGTNACC 3 cut(s) 1696, 1864, 2258
EcoRI GAATTC 2 cut(s) 532, 1559
EcoRII CCWGG 4 cut(s) 1263, 1893, 2242, 2407
EcoT14I CCWWGG 3 cut(s) 903, 1392, 2237
EcoT22I ATGCAT 1 cut(s) 2620
EcoT38I GRGCYC 2 cut(s) 255, 1289
EgeI GGCGCC 1 cut(s) 489
EheI GGCGCC 1 cut(s) 489
ErhI CCWWGG 3 cut(s) 903, 1392, 2237
Esp3I CGTCTC 2 cut(s) 199, 637
FalI AAGNNNNNCTT 4 cut(s) 20, 52, 1295, 1327
FaqI GGGAC 4 cut(s) 65, 79, 1417, 1829
FauI CCCGC 4 cut(s) 219, 1419, 2277, 2502
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 764
FokI GGATG 5 cut(s) 313, 1054, 1328, 1404, 1625
FriOI GRGCYC 2 cut(s) 255, 1289
FspBI CTAG 7 cut(s) 678, 741, 1320, 1332, 1518, 2421, 2791
GlaI GCGC 3 cut(s) 309, 489, 754
GsuI CTGGAG 2 cut(s) 1359, 1869
HaeII RGCGCY 1 cut(s) 491
HaeIII GGCC 9 cut(s) 57, 230, 305, 362, 434, 596, 1893, 1971, 2242
HgaI GACGC 5 cut(s) 181, 402, 412, 712, 2142
HhaI GCGC 3 cut(s) 310, 490, 755
Hin1I GRCGYC 5 cut(s) 213, 394, 423, 488, 704
Hin6I GCGC 3 cut(s) 308, 488, 753
HinP1I GCGC 3 cut(s) 308, 488, 753
HincII GTYRAC 2 cut(s) 765, 973
HindII GTYRAC 2 cut(s) 765, 973
HindIII AAGCTT 4 cut(s) 585, 1470, 1802, 2682
Hpy166II GTNNAC 7 cut(s) 765, 774, 838, 973, 1106, 1840, 2092
Hpy8I GTNNAC 7 cut(s) 765, 774, 838, 973, 1106, 1840, 2092
HpyCH4IV ACGT 5 cut(s) 213, 656, 717, 1545, 2308
HpyF3I CTNAG 5 cut(s) 684, 1460, 1485, 1781, 2292
HpySE526I ACGT 5 cut(s) 213, 656, 717, 1545, 2308
Hsp92I GRCGYC 5 cut(s) 213, 394, 423, 488, 704
HspAI GCGC 3 cut(s) 308, 488, 753
KasI GGCGCC 1 cut(s) 487
Kpn2I TCCGGA 1 cut(s) 1441
KpnI GGTACC 1 cut(s) 1004
Ksp22I TGATCA 1 cut(s) 3
LguI GCTCTTC 1 cut(s) 1519
LmnI GCTCC 9 cut(s) 10, 22, 511, 517, 679, 1010, 1240, 1793, 2150
Lsp1109I GCAGC 6 cut(s) 210, 767, 1140, 1401, 1858, 1885
LweI GCATC 8 cut(s) 540, 945, 1306, 1426, 1603, 1786, 2348, 2627
MaeI CTAG 7 cut(s) 678, 741, 1320, 1332, 1518, 2421, 2791
MaeII ACGT 5 cut(s) 213, 656, 717, 1545, 2308
MaeIII GTNAC 9 cut(s) 436, 1137, 1696, 1738, 1864, 2059, 2104, 2258, 2701
MbiI CCGCTC 1 cut(s) 25
MflI RGATCY 1 cut(s) 2509
MhlI GDGCHC 5 cut(s) 255, 1289, 1557, 1595, 2766
MlsI TGGCCA 2 cut(s) 362, 1893
MluNI TGGCCA 2 cut(s) 362, 1893
Mly113I GGCGCC 1 cut(s) 488
MlyI GAGTC 2 cut(s) 101, 2558
MmeI TCCRAC 2 cut(s) 171, 842
Mox20I TGGCCA 2 cut(s) 362, 1893
Mph1103I ATGCAT 1 cut(s) 2620
MroI TCCGGA 1 cut(s) 1441
MroXI GAANNNNTTC 1 cut(s) 2533
MscI TGGCCA 2 cut(s) 362, 1893
MseI TTAA 5 cut(s) 804, 1712, 1958, 1983, 2040
MslI CAYNNNNRTG 3 cut(s) 299, 1358, 1611
Msp20I TGGCCA 2 cut(s) 362, 1893
MspA1I CMGCKG 5 cut(s) 1153, 1414, 1785, 1871, 2474
Mva1269I GAATGC 1 cut(s) 2284
MvaI CCWGG 4 cut(s) 1265, 1895, 2244, 2409
MvnI CGCG 2 cut(s) 308, 671
NarI GGCGCC 1 cut(s) 488
NciI CCSGG 7 cut(s) 97, 98, 312, 522, 985, 2077, 2766
NcoI CCATGG 3 cut(s) 903, 1392, 2237
NmeAIII GCCGAG 2 cut(s) 274, 572
NmuCI GTSAC 7 cut(s) 1137, 1696, 1864, 2059, 2104, 2258, 2701
NsiI ATGCAT 1 cut(s) 2620
NspI RCATGY 2 cut(s) 178, 1878
NspV TTCGAA 2 cut(s) 404, 530
PaeI GCATGC 1 cut(s) 1878
PagI TCATGA 2 cut(s) 1279, 1855
PciSI GCTCTTC 1 cut(s) 1519
PcsI WCGNNNNNNNCGW 7 cut(s) 60, 72, 189, 272, 432, 536, 662
PctI GAATGC 1 cut(s) 2284
PdmI GAANNNNTTC 1 cut(s) 2533
PfeI GAWTC 8 cut(s) 1270, 1999, 2072, 2198, 2373, 2534, 2591, 2663
PflFI GACNNNGTC 3 cut(s) 380, 1349, 1544
PflMI CCANNNNNTGG 1 cut(s) 2243
PfoI TCCNGGA 1 cut(s) 2075
PinAI ACCGGT 1 cut(s) 2018
Ple19I CGATCG 2 cut(s) 508, 669
PleI GAGTC 2 cut(s) 100, 2557
PluTI GGCGCC 1 cut(s) 491
PpsI GAGTC 2 cut(s) 100, 2557
PpuMI RGGWCCY 1 cut(s) 33
PshAI GACNNNNGTC 1 cut(s) 168
PshBI ATTAAT 1 cut(s) 2040
Psp1406I AACGTT 1 cut(s) 2308
Psp5II RGGWCCY 1 cut(s) 33
Psp6I CCWGG 4 cut(s) 1263, 1893, 2242, 2407
PspEI GGTNACC 3 cut(s) 1696, 1864, 2258
PspGI CCWGG 4 cut(s) 1263, 1893, 2242, 2407
PspPPI RGGWCCY 1 cut(s) 33
PstNI CAGNNNCTG 1 cut(s) 2336
PsuI RGATCY 1 cut(s) 2509
PsyI GACNNNGTC 3 cut(s) 380, 1349, 1544
PvuI CGATCG 2 cut(s) 508, 669
RgaI GCGATCGC 1 cut(s) 669
RseI CAYNNNNRTG 3 cut(s) 299, 1358, 1611
SalI GTCGAC 1 cut(s) 763
SapI GCTCTTC 1 cut(s) 1519
SaqAI TTAA 5 cut(s) 804, 1712, 1958, 1983, 2040
ScaI AGTACT 1 cut(s) 1620
SchI GAGTC 2 cut(s) 101, 2558
SduI GDGCHC 5 cut(s) 255, 1289, 1557, 1595, 2766
SfaAI GCGATCGC 1 cut(s) 669
SfaNI GCATC 8 cut(s) 540, 945, 1306, 1426, 1603, 1786, 2348, 2627
SfoI GGCGCC 1 cut(s) 489
SfuI TTCGAA 2 cut(s) 404, 530
SgfI GCGATCGC 1 cut(s) 669
SgrAI CRCCGGYG 1 cut(s) 937
SinI GGWCC 9 cut(s) 33, 561, 1162, 1296, 1624, 1635, 2563, 2643, 2783
SmaI CCCGGG 1 cut(s) 98
SmiMI CAYNNNNRTG 3 cut(s) 299, 1358, 1611
SmlI CTYRAG 1 cut(s) 2349
SmoI CTYRAG 1 cut(s) 2349
SphI GCATGC 1 cut(s) 1878
SspDI GGCGCC 1 cut(s) 487
SspMI CTAG 7 cut(s) 678, 741, 1320, 1332, 1518, 2421, 2791
StyI CCWWGG 3 cut(s) 903, 1392, 2237
TaiI ACGT 5 cut(s) 216, 659, 720, 1548, 2311
TatI WGTACW 5 cut(s) 1105, 1618, 1935, 2175, 2317
TfiI GAWTC 8 cut(s) 1270, 1999, 2072, 2198, 2373, 2534, 2591, 2663
Tru1I TTAA 5 cut(s) 804, 1712, 1958, 1983, 2040
Tru9I TTAA 5 cut(s) 804, 1712, 1958, 1983, 2040
TscAI CASTG 2 cut(s) 1777, 2197
TseFI GTSAC 7 cut(s) 1137, 1696, 1864, 2059, 2104, 2258, 2701
TseI GCWGC 6 cut(s) 223, 755, 1153, 1414, 1871, 1898
Tsp45I GTSAC 7 cut(s) 1137, 1696, 1864, 2059, 2104, 2258, 2701
TspDTI ATGAA 7 cut(s) 59, 820, 1346, 1647, 1844, 1872, 2676
TspGWI ACGGA 4 cut(s) 190, 782, 2370, 2546
TspMI CCCGGG 1 cut(s) 96
TspRI CASTG 2 cut(s) 1777, 2197
Tth111I GACNNNGTC 3 cut(s) 380, 1349, 1544
Van91I CCANNNNNTGG 1 cut(s) 2243
VpaK11BI GGWCC 9 cut(s) 33, 561, 1162, 1296, 1624, 1635, 2563, 2643, 2783
VspI ATTAAT 1 cut(s) 2040
XagI CCTNNNNNAGG 1 cut(s) 2575
XapI RAATTY 4 cut(s) 532, 1559, 2520, 2740
XceI RCATGY 2 cut(s) 178, 1878
XmaI CCCGGG 1 cut(s) 96
XmiI GTMKAC 1 cut(s) 764
XmnI GAANNNNTTC 1 cut(s) 2533
XspI CTAG 7 cut(s) 678, 741, 1320, 1332, 1518, 2421, 2791
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 1 cut(s) 1620
Zsp2I ATGCAT 1 cut(s) 2620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.