MD04G1127300.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012301
Physical Location & Seq
Reverse (-)
21317939 .. 21321916
3978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1127300.v1.1.491

Sequence Viewer

Length: 2919 bp
ATGAAGATGGAGCGGAAAAAGACGTTGACGATGAACTGGGACAGCCTCCGGGACGACGATGACGATGATCGCTTTTTCGAGTCAAAGGAACGCGCCTCTTCCGTCGTTCCTCTCGACCTCGAGGAATCCTCATCCGAAGATGAGGATTTCGACGACAGTCGCAATTCATTTGCCTCATGTGTCTCATATGTTCGAAACGATGTTGATTTTCGTGCCTCCACCGCAGCCGCTCCGATGGACCCACCGTCTACTTCTTCGGCTCCAATGACGCCGAATTACAACATTTGGATGGAGGCACCGAGGTCTATCACTGAACGGCGAAAACGATTATTGCAGGGCATGGGGTTAGCCCAAGGACCTGGAAAAGACCTCGTTAGCTTTAAACAACTAACCAACATTAAACGTCTCGTTTCGAACAAAATCACAAACGGCATTGTTCCAATCCCTCGGTCAATCACTCGGGCTAAAGAAAAAGTACAAGTATCGTCGAATCAGCCGGTATCGGAGATAGAGCCAGAGTCGGAGCCTGAGCCTGATCATGAACCATTGTCGGTGATGCTCGTCCGTTCGAGGTCGGAGGGTGACATTGAAGCTTTCTCGATAAATAAACAAAGGAAGGAAGAGATAATCGAAACAATCTCGAAGCAACGACTTACGAGGACTTGCTCCGCCATAGCCGTAACTCGCTCGAGAATATGTCCGTACACCGAATCGATTAAAGTGTCAACTAACGAAGTCAGCGACTCTACCCCCAATAGTCGATTGGTTCGGACTAGCGGTGGATTGTCGTCGGTTTTCTTGAATAACCAGTTAGGAGCCTTCTTCTTGATTAAGAATCTGGACACAGGGACTAAGTTCATCGTGAACGAGTATGATCAGGATGGGTGCTGGAATCGACTCAGCGATCTCCAAACAGGGAAGCAATTGACGATGGACGAGTTCGAGAAATGTGTCGGGTACTCGCCAGTGGTGAAAGAGGTTATGCGAAGACAGAGCGTTTCCAGCGAAGGCAGCGGCGATAACAGGAAGGTCGGTGCAGGGGCAAATTCATTTGTTTCGAAGAGCCTGAGGATGAGCAAGAGAAGAGGGGTTGCTCTGTTGAAGAACAGTATACGAGGCGTGGGGACCGCTGTGAGTGTGTTGATTGGTGAGAAAGAGAGGGAAAACACGACGCCATCGCTAACATTGCCGCCGGAGCAGAAACCCGTCAAGAACTCGAGTTCTTCTTCGTCAGCTTCATCGGAATGGGTCAAAGTTAGGCAGACAGGGAAGCATTACAAGGAGTTATCGGCATTGCATTTGTGTCAAGAGATTCAAGCTCACGAGGGGTCAATTTGGGCTATTAAGTTTAGCTTGGATGCAAGGTTTCTTGCGAGTGCGGGAGAGGATCGAATTATTCAAGTGTGGGAAGTGCAAGAGTGTGAGATTATGTCATTGGATGGGAATTCGACCCCGCTTCATCACTCTTTCAGGTCTTCCACCCCTGATCGTTCCTTCACCCCTGATCGTTCTCCATCAATGTCTGATCAAGCTGTGGTTGTGCCATATGAGAAGGAGAATAAGAATAAGGAAAAAAGTGGGTCTGCTAGAAAAAGCAATCCAATCCCAGAATATGTGCATGTGCCCGAAACAGTGTTTTCATTTTCGGAGAAACCATTTTGCTCTTTTGAAGGTCATTTGGATGCTGTTCTGGACCTGTCATGGTCCAGAGATCAGCTATTGCTTTCATCTTCAATGGACAAAACTGTTAGGTTATGGGATTTGGAGACCAAGACTTGTTTAAAGTTGTTTGCCCACAATGATTATGTGACTTGCATACAATTCAATCCTTTGGATCACAATTACTTTATTAGTGGCTCACTCGATGCAAAGATTCGATTGTGGAACGTAGCTGTTAGGCAAGTCGTGGATTGGATTGATCTTTATGAAATGGTCACTGCTGCTTCCTATACTCCAGATGGCCAGGCTTGCATCATTGGTTCACACAAAGGAAAATGTCGTATGTACAATGCAGAAGATTGTAAATTAAGTCAGCATAGTCAGATTGATATTCAAAACAAGAAGAAGAATCAAGCCAAAAAGATTACAGGTTTCCAGTTTTCACCGATAAACCCAACTGAAATGCTTGTCACGTCTGCTGATTCTCAGATTCGGATTTTGGATGGCACAAACGTGACTCACAAGTTTAGAGGTTTTCGAAATATGAGTAGCCAAATTGGAGCTTCATTCACTCCAAACGGGAAGTACGTCATATGTGCTAGTGAAGACTCTCATGTATACATTTGGAGGCGGGATGAGCCCCGAATTTCAGCCACAGGGAAAAAAAGCATCACTAGCACCAATTCCCACGAACATTTTCAGTGTAAAGATGTTTCAGTAGCAATCCCATGGCCAGGCACCATAAAGGGTGACCCTCCATCTGTCATTGTGCAACATTCCAAGAGACAATCAAAATGCTCAACGCAACAACCACAATCTGGCAATTCATCCCTTACTCAAGAAGATGCAGGTGTAAGCAAGAGAGTGCTACCACCTATTCCGAAGAAAAACAACAAAGACAACAACAACAAGGGAAATGCATCGGATCAAATACCTTTAACTCCGCCGCAGGAAGATCAAGATCCAGCTCAAATATGCCGAACAGAATCTGGGATTGGAGAGTCGTTCAATTCGGACCCTTCCTCTATTAGGTATGGTGATTCAAGTTCTATGTCCGCCGGTAGTGCCTCCACATCATCTTGGTCTTCCTCTTGGTCATGGCTTGACAATATAGGCAACAACAATGCTAGCCAAACAATGCAAGCAACAGCTTGGGGTTTGGTAATTGTGACAACCACTTTAGACGGAGAAATCAGAGCATACCAAAATTTTGGGCTGCCGCGAAAAGTACAGAATAATATTTTCGGAGGCCCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

973

Amino Acids

108.12

Weight (kDa)

7.51

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 428 - 672 6.7e-19 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 429 - 475 4.3e-07 THOC3 beta-propeller domain
WD40 PF00400 435 - 469 7.1e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 549 - 630 4.4e-14 WDR3 first beta-propeller domain
WD40 PF00400 551 - 587 2.7e-07 WD domain, G-beta repeat
EIF3I PF24805 551 - 751 7.4e-10 EIF3I
Beta-prop_WDR5 PF25175 553 - 771 8.8e-39 WDR5 beta-propeller domain
WD40_Prp19 PF24814 553 - 673 2.9e-15 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 553 - 648 2.6e-11 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 555 - 595 9.7e-07 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 555 - 612 2.1e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_IFT122_1st PF23381 556 - 670 1.2e-07 IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 556 - 636 6.3e-11 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 559 - 677 2.7e-07 WDR55
Beta-prop_THOC3 PF25174 559 - 766 7.9e-27 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 561 - 685 3.3e-08 Aladin seven-bladed propeller
WD40_Gbeta PF25391 570 - 762 1.5e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 574 - 675 8.1e-08 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 602 - 766 1.1e-11 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 613 - 783 3e-14 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37670 AT2G37670 AT5G02430
fragaria_vesca FvH4_6g13960
malus_domestica MD04G1127300.v1.1 MD12G1140500.v1.1
prunus_persica Prupe.6G253900_v2.0.a1 Prupe.6G253900_v2.0.a1
pyrus_communis pycom04g11410
rosa_chinensis RchiOBHm_Chr3g0466781
rosa_laevigata RLG00000024553
rosa_multiflora Rmu_sc0008017.1_g000009
rosa_roxburghii Rroxscaffold_6G00414170
rosa_rugosa Rorug03G0083800
rosa_samantha Rh3AG131000 Rh3BG152000 Rh3CG151900 Rh3DG152700
rosa_wichuraiana Rw3G012380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2498
AbsI CCTCGAGG 1 cut(s) 119
Acc36I ACCTGC 1 cut(s) 2498
AccB1I GGYRCC 2 cut(s) 295, 2396
AccB7I CCANNNNNTGG 1 cut(s) 2477
AccBSI CCGCTC 2 cut(s) 13, 230
AccI GTMKAC 3 cut(s) 248, 1111, 2277
AccII CGCG 2 cut(s) 93, 2881
AclWI GGATC 4 cut(s) 1395, 1842, 2592, 2615
AcoI YGGCCR 2 cut(s) 1960, 2390
AcsI RAATTY 4 cut(s) 1045, 1444, 2304, 2866
AcyI GRCGYC 2 cut(s) 269, 1172
AfaI GTAC 6 cut(s) 477, 704, 959, 2006, 2246, 2889
AfiI CCNNNNNNNGG 3 cut(s) 2393, 2477, 2688
AhdI GACNNNNNGTC 1 cut(s) 244
AjiI CACGTC 1 cut(s) 2133
AjnI CCWGG 3 cut(s) 358, 1962, 2392
AjuI GAANNNNNNNTTGG 2 cut(s) 2637, 2669
AleI CACNNNNGTG 1 cut(s) 2171
Alw26I GTCTC 4 cut(s) 187, 410, 1760, 2437
AlwI GGATC 4 cut(s) 1395, 1842, 2592, 2615
AlwNI CAGNNNCTG 1 cut(s) 2648
Ama87I CYCGRG 4 cut(s) 119, 459, 688, 1216
AoxI GGCC 3 cut(s) 1960, 2390, 2908
ApeKI GCWGC 4 cut(s) 224, 1011, 1940, 2875
ApoI RAATTY 4 cut(s) 1045, 1444, 2304, 2866
Asp700I GAANNNNTTC 1 cut(s) 2355
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 7 cut(s) 238, 356, 1125, 1693, 1704, 2674, 2909
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 8 cut(s) 565, 593, 982, 1160, 1489, 2094, 2420, 2708
AsuII TTCGAA 4 cut(s) 193, 413, 1058, 2197
AsuNHI GCTAGC 1 cut(s) 2786
AvaI CYCGRG 4 cut(s) 119, 459, 688, 1216
AvaII GGWCC 6 cut(s) 238, 356, 1125, 1693, 1704, 2674
AxyI CCTNAGG 1 cut(s) 1067
BaeGI GKGCMC 1 cut(s) 1626
BalI TGGCCA 2 cut(s) 1962, 2392
BanI GGYRCC 2 cut(s) 295, 2396
BanII GRGCYC 1 cut(s) 2301
BauI CACGAG 1 cut(s) 1322
BbsI GAAGAC 4 cut(s) 994, 1467, 2271, 2736
BbvI GCAGC 4 cut(s) 236, 1023, 1927, 2862
BceAI ACGGC 3 cut(s) 332, 445, 662
BciT130I CCWGG 3 cut(s) 360, 1964, 2394
BclI TGATCA 3 cut(s) 535, 874, 1525
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 4 cut(s) 187, 410, 1760, 2437
BfaI CTAG 5 cut(s) 774, 1587, 2259, 2334, 2787
BfuAI ACCTGC 1 cut(s) 2498
BisI GCNGC 9 cut(s) 225, 228, 1012, 1015, 1190, 1941, 2606, 2876, 2879
BlsI GCNGC 9 cut(s) 226, 229, 1013, 1016, 1191, 1942, 2607, 2877, 2880
Bme1390I CCNGG 4 cut(s) 50, 360, 1964, 2394
Bme18I GGWCC 6 cut(s) 238, 356, 1125, 1693, 1704, 2674
BmeRI GACNNNNNGTC 1 cut(s) 244
BmeT110I CYCGRG 4 cut(s) 119, 459, 688, 1216
BmgBI CACGTC 1 cut(s) 2133
BmgT120I GGNCC 7 cut(s) 238, 356, 1125, 1693, 1704, 2674, 2909
BmiI GGNNCC 8 cut(s) 240, 261, 297, 525, 817, 1126, 2398, 2676
BmrFI CCNGG 4 cut(s) 50, 360, 1964, 2394
BmrI ACTGGG 1 cut(s) 46
BmsI GCATC 8 cut(s) 546, 1348, 1672, 1855, 1980, 2337, 2494, 2588
BmtI GCTAGC 1 cut(s) 2790
BmuI ACTGGG 1 cut(s) 46
BoxI GACNNNNGTC 1 cut(s) 156
BpiI GAAGAC 4 cut(s) 994, 1467, 2271, 2736
BplI GAGNNNNNCTC 2 cut(s) 113, 145
BpmI CTGGAG 1 cut(s) 1938
Bpu10I CCTNAGC 1 cut(s) 528
Bpu14I TTCGAA 4 cut(s) 193, 413, 1058, 2197
BpuEI CTTGAG 1 cut(s) 2481
BpuMI CCSGG 1 cut(s) 50
Bsa29I ATCGAT 1 cut(s) 713
BsaBI GATNNNNATC 1 cut(s) 2619
BsaHI GRCGYC 2 cut(s) 269, 1172
BsaI GGTCTC 1 cut(s) 1760
BsaJI CCNNGG 4 cut(s) 299, 352, 446, 2387
Bsc4I CCNNNNNNNGG 3 cut(s) 2393, 2477, 2688
Bse118I RCCGGY 2 cut(s) 496, 2717
Bse1I ACTGG 4 cut(s) 41, 808, 965, 2095
Bse21I CCTNAGG 1 cut(s) 1067
Bse3DI GCAATG 2 cut(s) 1184, 1292
Bse8I GATNNNNATC 1 cut(s) 2619
BseBI CCWGG 3 cut(s) 360, 1964, 2394
BseCI ATCGAT 1 cut(s) 713
BseDI CCNNGG 4 cut(s) 299, 352, 446, 2387
BseJI GATNNNNATC 1 cut(s) 2619
BseLI CCNNNNNNNGG 3 cut(s) 2393, 2477, 2688
BseMI GCAATG 2 cut(s) 1184, 1292
BseMII CTCAG 4 cut(s) 519, 913, 1058, 2159
BseNI ACTGG 4 cut(s) 41, 808, 965, 2095
BseSI GKGCMC 1 cut(s) 1626
BseXI GCAGC 4 cut(s) 236, 1023, 1927, 2862
BsgI GTGCAG 1 cut(s) 1056
Bsh1236I CGCG 2 cut(s) 93, 2881
BshFI GGCC 3 cut(s) 1962, 2392, 2910
BshNI GGYRCC 2 cut(s) 295, 2396
BshVI ATCGAT 1 cut(s) 713
BsiHKCI CYCGRG 4 cut(s) 119, 459, 688, 1216
BsiSI CCGG 4 cut(s) 49, 497, 1193, 2718
BslFI GGGAC 4 cut(s) 53, 65, 862, 1138
BslI CCNNNNNNNGG 3 cut(s) 2393, 2477, 2688
BsmAI GTCTC 4 cut(s) 187, 410, 1760, 2437
BsmBI CGTCTC 1 cut(s) 410
BsmFI GGGAC 4 cut(s) 53, 65, 862, 1138
BsnI GGCC 3 cut(s) 1962, 2392, 2910
Bso31I GGTCTC 1 cut(s) 1760
BsoBI CYCGRG 4 cut(s) 119, 459, 688, 1216
Bsp119I TTCGAA 4 cut(s) 193, 413, 1058, 2197
Bsp1286I GDGCHC 2 cut(s) 1626, 2301
Bsp1407I TGTACA 1 cut(s) 2004
Bsp19I CCATGG 1 cut(s) 2387
BspANI GGCC 3 cut(s) 1962, 2392, 2910
BspCNI CTCAG 4 cut(s) 520, 912, 1059, 2158
BspDI ATCGAT 1 cut(s) 713
BspFNI CGCG 2 cut(s) 93, 2881
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 8 cut(s) 240, 261, 297, 525, 817, 1126, 2398, 2676
BspMI ACCTGC 1 cut(s) 2498
BspOI GCTAGC 1 cut(s) 2790
BspPI GGATC 4 cut(s) 1395, 1842, 2592, 2615
BspQI GCTCTTC 1 cut(s) 1055
BspT104I TTCGAA 4 cut(s) 193, 413, 1058, 2197
BspT107I GGYRCC 2 cut(s) 295, 2396
BspTNI GGTCTC 1 cut(s) 1760
BsrBI CCGCTC 2 cut(s) 13, 230
BsrDI GCAATG 2 cut(s) 1184, 1292
BsrFI RCCGGY 2 cut(s) 496, 2717
BsrGI TGTACA 1 cut(s) 2004
BsrI ACTGG 4 cut(s) 41, 808, 965, 2095
BssAI RCCGGY 2 cut(s) 496, 2717
BssECI CCNNGG 4 cut(s) 299, 352, 446, 2387
BssNAI GTATAC 2 cut(s) 1112, 2278
BssNI GRCGYC 2 cut(s) 269, 1172
BssSI CACGAG 1 cut(s) 1322
BssT1I CCWWGG 2 cut(s) 352, 2387
Bst1107I GTATAC 2 cut(s) 1112, 2278
Bst2BI CACGAG 1 cut(s) 1322
Bst2UI CCWGG 3 cut(s) 360, 1964, 2394
Bst4CI ACNGT 5 cut(s) 158, 246, 1109, 1633, 1747
Bst6I CTCTTC 4 cut(s) 103, 615, 1055, 1078
BstACI GRCGYC 2 cut(s) 269, 1172
BstAUI TGTACA 1 cut(s) 2004
BstBI TTCGAA 4 cut(s) 193, 413, 1058, 2197
BstC8I GCNNGC 3 cut(s) 1969, 2788, 2802
BstDEI CTNAG 5 cut(s) 528, 852, 899, 1067, 2145
BstDSI CCRYGG 1 cut(s) 2387
BstEII GGTNACC 1 cut(s) 2408
BstENI CCTNNNNNAGG 1 cut(s) 2686
BstFNI CGCG 2 cut(s) 93, 2881
BstHHI GCGC 1 cut(s) 95
BstMAI GTCTC 4 cut(s) 187, 410, 1760, 2437
BstMWI GCNNNNNNNGC 9 cut(s) 221, 1002, 1011, 1186, 1195, 1968, 2296, 2334, 2723
BstNI CCWGG 3 cut(s) 360, 1964, 2394
BstNSI RCATGY 1 cut(s) 1622
BstPAI GACNNNNGTC 1 cut(s) 156
BstPI GGTNACC 1 cut(s) 2408
BstSCI CCNGG 4 cut(s) 48, 358, 1962, 2392
BstSLI GKGCMC 1 cut(s) 1626
BstUI CGCG 2 cut(s) 93, 2881
BstV1I GCAGC 4 cut(s) 236, 1023, 1927, 2862
BstV2I GAAGAC 4 cut(s) 994, 1467, 2271, 2736
BstX2I RGATCY 1 cut(s) 2620
BstXI CCANNNNNNTGG 2 cut(s) 359, 2870
BstYI RGATCY 1 cut(s) 2620
BstZ17I GTATAC 2 cut(s) 1112, 2278
Bsu15I ATCGAT 1 cut(s) 713
Bsu36I CCTNAGG 1 cut(s) 1067
BsuRI GGCC 3 cut(s) 1962, 2392, 2910
BsuTUI ATCGAT 1 cut(s) 713
BtgI CCRYGG 1 cut(s) 2387
BtgZI GCGATG 1 cut(s) 1161
BtrI CACGTC 1 cut(s) 2133
BtsI GCAGTG 1 cut(s) 1935
BtsIMutI CAGTG 5 cut(s) 309, 972, 1638, 1935, 2366
BveI ACCTGC 1 cut(s) 2498
Cac8I GCNNGC 3 cut(s) 1969, 2788, 2802
CaiI CAGNNNCTG 1 cut(s) 2648
CciI TCATGA 1 cut(s) 538
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 2 cut(s) 496, 2717
Cfr13I GGNCC 7 cut(s) 238, 356, 1125, 1693, 1704, 2674, 2909
ClaI ATCGAT 1 cut(s) 713
CseI GACGC 2 cut(s) 277, 1180
Csp6I GTAC 6 cut(s) 476, 703, 958, 2005, 2245, 2888
CviAII CATG 8 cut(s) 177, 340, 539, 1619, 1701, 2273, 2388, 2757
CviQI GTAC 6 cut(s) 476, 703, 958, 2005, 2245, 2888
DdeI CTNAG 5 cut(s) 528, 852, 899, 1067, 2145
DraI TTTAAA 2 cut(s) 382, 1782
DriI GACNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 2 cut(s) 1960, 2390
Eam1104I CTCTTC 4 cut(s) 103, 615, 1055, 1078
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 4 cut(s) 103, 615, 1055, 1078
EciI GGCGGA 3 cut(s) 658, 2592, 2704
Eco130I CCWWGG 2 cut(s) 352, 2387
Eco24I GRGCYC 1 cut(s) 2301
Eco31I GGTCTC 1 cut(s) 1760
Eco47I GGWCC 6 cut(s) 238, 356, 1125, 1693, 1704, 2674
Eco81I CCTNAGG 1 cut(s) 1067
Eco88I CYCGRG 4 cut(s) 119, 459, 688, 1216
Eco91I GGTNACC 1 cut(s) 2408
EcoNI CCTNNNNNAGG 1 cut(s) 2686
EcoO109I RGGNCCY 2 cut(s) 356, 2909
EcoO65I GGTNACC 1 cut(s) 2408
EcoRI GAATTC 1 cut(s) 1444
EcoRII CCWGG 3 cut(s) 358, 1962, 2392
EcoT14I CCWWGG 2 cut(s) 352, 2387
EcoT22I ATGCAT 1 cut(s) 2581
EcoT38I GRGCYC 1 cut(s) 2301
ErhI CCWWGG 2 cut(s) 352, 2387
Esp3I CGTCTC 1 cut(s) 410
FaeI CATG 8 cut(s) 180, 343, 542, 1622, 1704, 2276, 2391, 2760
FalI AAGNNNNNCTT 2 cut(s) 1337, 1369
FaqI GGGAC 4 cut(s) 53, 65, 862, 1138
FatI CATG 8 cut(s) 176, 339, 538, 1618, 1700, 2272, 2387, 2756
FauI CCCGC 3 cut(s) 1372, 1461, 2283
FauNDI CATATG 3 cut(s) 187, 1546, 2252
FbaI TGATCA 3 cut(s) 535, 874, 1525
FblI GTMKAC 3 cut(s) 248, 1111, 2277
Fnu4HI GCNGC 9 cut(s) 225, 228, 1012, 1015, 1190, 1941, 2606, 2876, 2879
FriOI GRGCYC 1 cut(s) 2301
Fsp4HI GCNGC 9 cut(s) 225, 228, 1012, 1015, 1190, 1941, 2606, 2876, 2879
FspBI CTAG 5 cut(s) 774, 1587, 2259, 2334, 2787
GlaI GCGC 1 cut(s) 94
GluI GCNGC 9 cut(s) 225, 228, 1012, 1015, 1190, 1941, 2606, 2876, 2879
GsuI CTGGAG 1 cut(s) 1938
HaeIII GGCC 3 cut(s) 1962, 2392, 2910
HapII CCGG 4 cut(s) 49, 497, 1193, 2718
HgaI GACGC 2 cut(s) 277, 1180
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 2 cut(s) 269, 1172
Hin1II CATG 8 cut(s) 180, 343, 542, 1622, 1704, 2276, 2391, 2760
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HincII GTYRAC 2 cut(s) 27, 726
HindII GTYRAC 2 cut(s) 27, 726
HindIII AAGCTT 1 cut(s) 591
HpaII CCGG 4 cut(s) 49, 497, 1193, 2718
HphI GGTGA 8 cut(s) 565, 593, 982, 1160, 1489, 2094, 2420, 2708
Hpy166II GTNNAC 8 cut(s) 27, 249, 705, 726, 865, 1112, 1982, 2278
Hpy8I GTNNAC 8 cut(s) 27, 249, 705, 726, 865, 1112, 1982, 2278
Hpy99I CGWCG 6 cut(s) 59, 107, 155, 490, 793, 1174
HpyAV CCTTC 8 cut(s) 610, 829, 1001, 1021, 1504, 1546, 1664, 2688
HpyCH4III ACNGT 5 cut(s) 158, 246, 1109, 1633, 1747
HpyCH4IV ACGT 6 cut(s) 23, 403, 1887, 2132, 2172, 2247
HpyF10VI GCNNNNNNNGC 9 cut(s) 221, 1002, 1011, 1186, 1195, 1968, 2296, 2334, 2723
HpyF3I CTNAG 5 cut(s) 528, 852, 899, 1067, 2145
HpySE526I ACGT 6 cut(s) 23, 403, 1887, 2132, 2172, 2247
Hsp92I GRCGYC 2 cut(s) 269, 1172
Hsp92II CATG 8 cut(s) 180, 343, 542, 1622, 1704, 2276, 2391, 2760
HspAI GCGC 1 cut(s) 93
Ksp22I TGATCA 3 cut(s) 535, 874, 1525
LguI GCTCTTC 1 cut(s) 1055
LmnI GCTCC 8 cut(s) 10, 235, 265, 523, 671, 815, 1195, 2219
Lsp1109I GCAGC 4 cut(s) 236, 1023, 1927, 2862
LweI GCATC 8 cut(s) 546, 1348, 1672, 1855, 1980, 2337, 2494, 2588
MaeI CTAG 5 cut(s) 774, 1587, 2259, 2334, 2787
MaeII ACGT 6 cut(s) 23, 403, 1887, 2132, 2172, 2247
MaeIII GTNAC 8 cut(s) 581, 679, 1807, 1933, 2128, 2173, 2408, 2827
MbiI CCGCTC 2 cut(s) 13, 230
MfeI CAATTG 1 cut(s) 923
MflI RGATCY 1 cut(s) 2620
MhlI GDGCHC 2 cut(s) 1626, 2301
MlsI TGGCCA 2 cut(s) 1962, 2392
MluNI TGGCCA 2 cut(s) 1962, 2392
MlyI GAGTC 7 cut(s) 89, 527, 737, 891, 2170, 2261, 2669
MmeI TCCRAC 2 cut(s) 501, 555
Mox20I TGGCCA 2 cut(s) 1962, 2392
Mph1103I ATGCAT 1 cut(s) 2581
MroXI GAANNNNTTC 1 cut(s) 2355
MscI TGGCCA 2 cut(s) 1962, 2392
MseI TTAA 9 cut(s) 381, 399, 717, 831, 1344, 1781, 2027, 2597, 2917
MslI CAYNNNNRTG 4 cut(s) 287, 1243, 1680, 2171
Msp20I TGGCCA 2 cut(s) 1962, 2392
MspA1I CMGCKG 2 cut(s) 1014, 1130
MspI CCGG 4 cut(s) 49, 497, 1193, 2718
MspR9I CCNGG 4 cut(s) 50, 360, 1964, 2394
MunI CAATTG 1 cut(s) 923
MvaI CCWGG 3 cut(s) 360, 1964, 2394
MvnI CGCG 2 cut(s) 93, 2881
MwoI GCNNNNNNNGC 9 cut(s) 221, 1002, 1011, 1186, 1195, 1968, 2296, 2334, 2723
NciI CCSGG 1 cut(s) 50
NcoI CCATGG 1 cut(s) 2387
NdeI CATATG 3 cut(s) 187, 1546, 2252
NheI GCTAGC 1 cut(s) 2786
NlaIII CATG 8 cut(s) 180, 343, 542, 1622, 1704, 2276, 2391, 2760
NlaIV GGNNCC 8 cut(s) 240, 261, 297, 525, 817, 1126, 2398, 2676
NmuCI GTSAC 7 cut(s) 581, 1807, 1933, 2128, 2173, 2408, 2827
NsiI ATGCAT 1 cut(s) 2581
NspI RCATGY 1 cut(s) 1622
NspV TTCGAA 4 cut(s) 193, 413, 1058, 2197
OliI CACNNNNGTG 1 cut(s) 2171
PaeR7I CTCGAG 3 cut(s) 119, 688, 1216
PagI TCATGA 1 cut(s) 538
PaqCI CACCTGC 1 cut(s) 2498
PciSI GCTCTTC 1 cut(s) 1055
PcsI WCGNNNNNNNCGW 5 cut(s) 60, 111, 322, 738, 2244
PdmI GAANNNNTTC 1 cut(s) 2355
PflMI CCANNNNNTGG 1 cut(s) 2477
PfoI TCCNGGA 1 cut(s) 48
PkrI GCNGC 9 cut(s) 226, 229, 1013, 1016, 1191, 1942, 2607, 2877, 2880
PleI GAGTC 7 cut(s) 88, 526, 737, 891, 2170, 2261, 2668
PpsI GAGTC 7 cut(s) 88, 526, 737, 891, 2170, 2261, 2668
PpuMI RGGWCCY 1 cut(s) 356
PshAI GACNNNNGTC 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 356
Psp6I CCWGG 3 cut(s) 358, 1962, 2392
PspEI GGTNACC 1 cut(s) 2408
PspGI CCWGG 3 cut(s) 358, 1962, 2392
PspN4I GGNNCC 8 cut(s) 240, 261, 297, 525, 817, 1126, 2398, 2676
PspPI GGNCC 7 cut(s) 238, 356, 1125, 1693, 1704, 2674, 2909
PspPPI RGGWCCY 1 cut(s) 356
PspXI VCTCGAGB 2 cut(s) 119, 1216
PstNI CAGNNNCTG 1 cut(s) 2648
PsuI RGATCY 1 cut(s) 2620
RsaI GTAC 6 cut(s) 477, 704, 959, 2006, 2246, 2889
RsaNI GTAC 6 cut(s) 476, 703, 958, 2005, 2245, 2888
RseI CAYNNNNRTG 4 cut(s) 287, 1243, 1680, 2171
SapI GCTCTTC 1 cut(s) 1055
SaqAI TTAA 9 cut(s) 381, 399, 717, 831, 1344, 1781, 2027, 2597, 2917
SatI GCNGC 9 cut(s) 225, 228, 1012, 1015, 1190, 1941, 2606, 2876, 2879
Sau96I GGNCC 7 cut(s) 238, 356, 1125, 1693, 1704, 2674, 2909
SchI GAGTC 7 cut(s) 89, 527, 737, 891, 2170, 2261, 2669
ScrFI CCNGG 4 cut(s) 50, 360, 1964, 2394
SduI GDGCHC 2 cut(s) 1626, 2301
SfaNI GCATC 8 cut(s) 546, 1348, 1672, 1855, 1980, 2337, 2494, 2588
Sfr274I CTCGAG 3 cut(s) 119, 688, 1216
SfuI TTCGAA 4 cut(s) 193, 413, 1058, 2197
SinI GGWCC 6 cut(s) 238, 356, 1125, 1693, 1704, 2674
SlaI CTCGAG 3 cut(s) 119, 688, 1216
SmiMI CAYNNNNRTG 4 cut(s) 287, 1243, 1680, 2171
SmlI CTYRAG 4 cut(s) 119, 688, 1216, 2496
SmoI CTYRAG 4 cut(s) 119, 688, 1216, 2496
SspI AATATT 1 cut(s) 2899
SspMI CTAG 5 cut(s) 774, 1587, 2259, 2334, 2787
StyD4I CCNGG 4 cut(s) 48, 358, 1962, 2392
StyI CCWWGG 2 cut(s) 352, 2387
TaaI ACNGT 5 cut(s) 158, 246, 1109, 1633, 1747
TaiI ACGT 6 cut(s) 26, 406, 1890, 2135, 2175, 2250
TaqII GACCGA 1 cut(s) 438
TatI WGTACW 3 cut(s) 475, 2004, 2887
TauI GCSGC 5 cut(s) 230, 1017, 1192, 2608, 2881
Tru1I TTAA 9 cut(s) 381, 399, 717, 831, 1344, 1781, 2027, 2597, 2917
Tru9I TTAA 9 cut(s) 381, 399, 717, 831, 1344, 1781, 2027, 2597, 2917
TscAI CASTG 5 cut(s) 316, 972, 1638, 1942, 2366
TseFI GTSAC 7 cut(s) 581, 1807, 1933, 2128, 2173, 2408, 2827
TseI GCWGC 4 cut(s) 224, 1011, 1940, 2875
Tsp45I GTSAC 7 cut(s) 581, 1807, 1933, 2128, 2173, 2408, 2827
TspGWI ACGGA 4 cut(s) 91, 554, 690, 2859
TspRI CASTG 5 cut(s) 316, 972, 1638, 1942, 2366
Van91I CCANNNNNTGG 1 cut(s) 2477
VpaK11BI GGWCC 6 cut(s) 238, 356, 1125, 1693, 1704, 2674
XagI CCTNNNNNAGG 1 cut(s) 2686
XapI RAATTY 4 cut(s) 1045, 1444, 2304, 2866
XceI RCATGY 1 cut(s) 1622
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XhoI CTCGAG 3 cut(s) 119, 688, 1216
XmiI GTMKAC 3 cut(s) 248, 1111, 2277
XmnI GAANNNNTTC 1 cut(s) 2355
XspI CTAG 5 cut(s) 774, 1587, 2259, 2334, 2787
Zsp2I ATGCAT 1 cut(s) 2581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.