Rh3AG131000

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0012301
Physical Location & Seq
Forward (+)
11347402 .. 11351702
4301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG131000.1

Sequence Viewer

Length: 2868 bp
ATGATCATGGAGCCGAAGATGGAGAGGAAGAGGACGTTCGCTATGAACTGGGACGGCCTCCACGACGAAGAAGACGACGACCGCTTCTTCGAGTCCCGGGAGAGAATCTCCTCCGCCGTGCCTATCGACCTCGAAGCCTCCTCCTCCGACGATGAGGACTTCGACGACAGTCGTATGTCCTTCGCCTCCTGCGTCTCCTCCGTCCGAAACGACGTCGAGGTGCTGCGAGCCTTGTCCGCCGCCGAGCCGTCCTTCGCCTCGTCGCCTCCGATGTCACCGGACTACAACATCTGGATGGCCGCGCCGGGGTCGATCTCCGAACGCCGCAGGCGGTTGTTGCAGGGCATGGGATTGGCCAACGGCGGCAAAGACATCGTCAGCTTCAGACGCCTCGTTTCGAATAAAGCCCCAAACGGCGTCTTTCCGGCCGTTACGAGTTGCCCTACTCGCTCTCGGGAAAAGATACAAGTTCCCGAAACAGCGCCCGAACCCGATCCCGATCCGGAGCCAGAGTCGGGGGCTTCGAATTCGCCTTTGCCGATGATGCTTGTACGGTCCAGATCGGAAGGAGAGATCGAAGCTTTCTCGGCCGATAAACGAAGGAAAGAGCAGGTAATTGGCACTGTCTCGAAACAACGGCTGACAAGAACGTCGTCGGCGATCGCGGCTCCTAGCTCTAGGATGTGTTTTTCCACGACGCCTCAAAGCGACGTGGGAACCAAGACCGTCCGATCTAGGCAAAACAGCGCCGCATTGTCGACGGAGTTCACCAACAATTTAGGGGCGTTCTTCTTGATTAAAAATCTGGACACGGGAACCGAGTTCATTGTGAACGAGTACGATCAAAACGGGTGTTGGAACAAGTTTAGTGATCTCCAAACGGGGAAGAAATTGACCGTGGACGAGTTTGAGAAGTCCGTCGGGTACTCGCCGGTGGTGAAGGAGGTGATGCGGCGGCAAAATGTCAACATTGAAGCCGGGATTGAGAGGAAGGTACCGGCAGGAGCCAATTCGTTTGTGTCAAAGAGTTTGAGGATGAGCAAGAGAAAGGTGGCTCTTTTGAAGAATAGCATAAAAGGTGTGGGAAGTGCTGTGAGTGTACTAATTGGGGATAAGGATCAACACGCCTCGTCGCCGCCTCCTCCGCTGCCGCAGGACCAGAAATCAAGCAAGGCTAATTCGGGGTCTGGTTCGGAGTGGATCAAAGTTAGGCAGACTGGGAAGTCATATAAGGAGCTATCAGCATTGCATTTGTGCCAGGAGATTCAGGCTCATGAGGGCTCAATTTGGACCATCAAGTTTAGCTTGGATGCTAGATTTCTTGCTAGTGCTGGAGAGGACAGGGTCATTCATGTATGGGAAGTTCAAGAATGTGAGATTATGTCCATGGATAGCAATGCGAACCCGCTGCATCCCTCCTCGGTGTCCCCATATCCGGATCGATCAATGTGTCTTAGCTCTGAAGCTTCAATTTCCTCTGAGAAGAAGAAGAAGGGGAAGGGAGGCTCTTCTAGGAAAACTAATCCCATTCCTGACTATGTCCATGTACCCGAATTCGTGTTTGCCCTCTCGGAGAAACCTGTGTGCTCTTTCGAAGGTCATTTGGATGCAGTACTGGACCTGTCATGGTCCAGAGATCAGCTATTGCTTTCATCTTCAATGGACAAAACTGTTAGATTATGGGATATGGTGACCAAGAGTTGTTTAAAGATGTTTGCCCACAATGATTATGTAACTTGCATACAGTTCAATCCATTGGACGACAGTGTTTTTCTCAGCGGCTCCCTTGATGCGAAGCTTCGATTATGGGACATATCTGATCGAAGAGTGGTGGACTGGACTGATCTTCATGAAATGGTCACCGCTGCATGCTATACTCCAGATGGCCAGGCTGCCATCATTGGCTCGCACAAAGGGAACTGTCGTATGTACAATGCAGAAGATTGTAAATTAAGTCAAGAAGGCCAGATTGATATTAAAAACAAGAAGAAGAATCAAGCAAAGAAAGTTACCGGTTTCCAGTTTTCTCCGATTAATCCATCTGAAATGCTTGTCACATCTGCTGATTCCCGGATTAGGATTGTGGACGGTAAAGATGTCACTCATAAGTTTAGAGGTTTCCGAAATACAAGCAGTCAAATTGGAGCGTCATTCACTCCCAATGGAAAGTACATTATATGTGCCAGTGAAGATTCTCATGTATATGTATGGAAGCGTGAAGAGCCTCGAGTGTCAGCCGCAGGAAAAAAGAGCACAATCACCACCACTTCTCATGAATATTTTCAGTGTAAAGATGTTTCAGTAGCAATCCCATGGCCTGGCACCATAAAAGGTGACCCTCCCCCGCCTGTGTCTGTGCATTCTTCTAAGAGGCACTCAAAACGTTCCTCTGTACAGCCAGTTTCAGGAACTGCATCACCTACTCAAGACGGAGGAGACAGCAAGAGATTCCTGCCACCTCTTCCAAAGAAAAACACTAATCCTAGTACTCCTCCTAGTGCAACAGCGGAGAAAACCACTCATCTTAGTACTCCTCCTAATGCGACAGCGGAGAAACCTTCGACTCCCCCACCAGAAGATCAGGATCCATCTGAAATTTCTCGAACGGATTCTGGGATTGGCGAGTCATTCAGCTCAGACCCTTCCTCTATTAGGTATGGTGGCGATTCACCTTCTATATCTTCTGGCAATGCATCATCATCTTGGTCTTCCTCTTGGTCCTCTTTGTTTGGGCATGAATCGAGCCAAACAACCCAAGCTTGGGGCTTGGTCATTGTGACAGCCACCCTGGAAGGTGAAATCAGGGCATATCAAAATTTTGGGATACCAAGAAGGGTGCCCGGCAGTTTTTTTGGAGGTCCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

955

Amino Acids

104.67

Weight (kDa)

7.54

Isoelectric Point (pI)

54.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 414 - 461 4.6e-07 THOC3 beta-propeller domain
WD40 PF00400 419 - 453 7.7e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 524 - 647 3e-10 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 525 - 588 1.7e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 525 - 645 5e-17 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 525 - 755 9.7e-20 Prp19 WD40 domain
WD40 PF00400 526 - 562 6.9e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 526 - 746 4.2e-38 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 527 - 620 6.7e-10 CDC20/Fizzy WD40 domain
EIF3I PF24805 528 - 727 2.8e-10 EIF3I
Beta-prop_THOC3 PF25174 529 - 571 1.7e-06 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 531 - 611 1.5e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_IFT122_1st PF23381 531 - 645 1.1e-06 IFT122 first beta-propeller
Beta-prop_THOC3 PF25174 534 - 741 5.6e-27 THOC3 beta-propeller domain
WDR55 PF24796 534 - 735 3.7e-08 WDR55
Beta-prop_Aladin PF25460 536 - 651 5.1e-10 Aladin seven-bladed propeller
WD40_Gbeta PF25391 545 - 645 3.1e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 549 - 649 1.6e-07 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 577 - 743 2.5e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 589 - 743 3.8e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 609 - 739 6.8e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37670 AT2G37670 AT5G02430
fragaria_vesca FvH4_6g13960
malus_domestica MD04G1127300.v1.1 MD12G1140500.v1.1
prunus_persica Prupe.6G253900_v2.0.a1 Prupe.6G253900_v2.0.a1
pyrus_communis pycom04g11410
rosa_chinensis RchiOBHm_Chr3g0466781
rosa_laevigata RLG00000024553
rosa_multiflora Rmu_sc0008017.1_g000009
rosa_roxburghii Rroxscaffold_6G00414170
rosa_rugosa Rorug03G0083800
rosa_samantha Rh3AG131000 Rh3BG152000 Rh3CG151900 Rh3DG152700
rosa_wichuraiana Rw3G012380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 649, 1222
AatII GACGTC 1 cut(s) 216
Acc36I ACCTGC 1 cut(s) 601
Acc65I GGTACC 1 cut(s) 994
AccB1I GGYRCC 3 cut(s) 994, 2321, 2836
AccB7I CCANNNNNTGG 1 cut(s) 2318
AccI GTMKAC 1 cut(s) 758
AccII CGCG 2 cut(s) 302, 665
AccIII TCCGGA 2 cut(s) 502, 1435
AclI AACGTT 1 cut(s) 2383
AclWI GGATC 7 cut(s) 488, 494, 1125, 1208, 1446, 2579, 2592
AcoI YGGCCR 5 cut(s) 297, 354, 426, 588, 1885
AcsI RAATTY 4 cut(s) 526, 1553, 2595, 2815
AcuI CTGAAG 2 cut(s) 367, 1482
AcyI GRCGYC 4 cut(s) 213, 388, 417, 698
AfiI CCNNNNNNNGG 9 cut(s) 306, 515, 1435, 2076, 2318, 2405, 2652, 2760, 2761
AgeI ACCGGT 1 cut(s) 2012
AgsI TTSAA 6 cut(s) 974, 1063, 1367, 1470, 1659, 1750
AjiI CACGTC 1 cut(s) 712
AjnI CCWGG 4 cut(s) 1257, 1887, 2317, 2787
Alw21I GWGCWC 2 cut(s) 1589, 2255
Alw26I GTCTC 3 cut(s) 199, 631, 2430
AlwI GGATC 7 cut(s) 488, 494, 1125, 1208, 1446, 2579, 2592
AlwNI CAGNNNCTG 1 cut(s) 2411
Ama87I CYCGRG 3 cut(s) 96, 453, 2226
Aor13HI TCCGGA 2 cut(s) 502, 1435
AoxI GGCC 8 cut(s) 55, 297, 354, 426, 588, 1885, 1963, 2315
ApeKI GCWGC 5 cut(s) 223, 1147, 1408, 1865, 1892
ApoI RAATTY 4 cut(s) 526, 1553, 2595, 2815
ArsI GACNNNNNNTTYG 4 cut(s) 71, 103, 360, 392
AseI ATTAAT 1 cut(s) 2034
AsiGI ACCGGT 1 cut(s) 2012
AsiSI GCGATCGC 1 cut(s) 663
Asp700I GAANNNNTTC 2 cut(s) 2280, 2608
Asp718I GGTACC 1 cut(s) 994
AspLEI GCGC 3 cut(s) 304, 484, 749
AspS9I GGNCC 7 cut(s) 555, 1156, 1290, 1618, 1629, 2718, 2858
AsuC2I CCSGG 6 cut(s) 97, 98, 306, 979, 2071, 2841
AsuII TTCGAA 3 cut(s) 398, 524, 1593
AvaI CYCGRG 3 cut(s) 96, 453, 2226
AvaII GGWCC 7 cut(s) 555, 1156, 1290, 1618, 1629, 2718, 2858
BaeGI GKGCMC 1 cut(s) 2841
BalI TGGCCA 2 cut(s) 356, 1887
BamHI GGATCC 1 cut(s) 2584
BanI GGYRCC 3 cut(s) 994, 2321, 2836
BanII GRGCYC 1 cut(s) 1283
BbsI GAAGAC 2 cut(s) 78, 2700
Bbv12I GWGCWC 2 cut(s) 1589, 2255
BbvI GCAGC 5 cut(s) 210, 1134, 1395, 1852, 1879
BccI CCATC 7 cut(s) 13, 289, 1301, 1877, 1904, 2047, 2596
BceAI ACGGC 7 cut(s) 70, 101, 232, 376, 413, 430, 653
BcgI CGANNNNNNTGC 4 cut(s) 355, 389, 1390, 1424
BciT130I CCWGG 4 cut(s) 1259, 1889, 2319, 2789
BciVI GTATCC 1 cut(s) 2817
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 6 cut(s) 97, 98, 306, 979, 2071, 2841
BcoDI GTCTC 3 cut(s) 199, 631, 2430
BfaI CTAG 9 cut(s) 672, 678, 735, 1314, 1326, 1512, 2484, 2496, 2866
BfoI RGCGCY 2 cut(s) 485, 750
BfuAI ACCTGC 1 cut(s) 601
BfuI GTATCC 1 cut(s) 2817
BmcAI AGTACT 3 cut(s) 1614, 2488, 2530
Bme18I GGWCC 7 cut(s) 555, 1156, 1290, 1618, 1629, 2718, 2858
BmeT110I CYCGRG 3 cut(s) 96, 453, 2226
BmgBI CACGTC 1 cut(s) 712
BmgT120I GGNCC 7 cut(s) 555, 1156, 1290, 1618, 1629, 2718, 2858
BmrI ACTGGG 2 cut(s) 58, 1227
BmsI GCATC 8 cut(s) 534, 939, 1300, 1420, 1597, 1780, 2423, 2702
BmuI ACTGGG 2 cut(s) 58, 1227
BoxI GACNNNNGTC 1 cut(s) 168
BpiI GAAGAC 2 cut(s) 78, 2700
BplI GAGNNNNNCTC 2 cut(s) 92, 124
BpmI CTGGAG 2 cut(s) 1353, 1863
Bpu14I TTCGAA 3 cut(s) 398, 524, 1593
BpuEI CTTGAG 1 cut(s) 2409
BpuMI CCSGG 6 cut(s) 97, 98, 306, 979, 2071, 2841
Bsa29I ATCGAT 1 cut(s) 1441
BsaBI GATNNNNATC 3 cut(s) 498, 1116, 2583
BsaHI GRCGYC 4 cut(s) 213, 388, 417, 698
BsaJI CCNNGG 7 cut(s) 96, 305, 897, 1386, 1419, 2312, 2787
BsaWI WCCGGW 4 cut(s) 277, 502, 1435, 2012
BsaXI ACNNNNNCTCC 2 cut(s) 497, 527
Bsc4I CCNNNNNNNGG 9 cut(s) 306, 515, 1435, 2076, 2318, 2405, 2652, 2760, 2761
Bse118I RCCGGY 3 cut(s) 931, 997, 2012
Bse1I ACTGG 7 cut(s) 53, 1222, 1620, 1841, 2020, 2184, 2399
Bse3DI GCAATG 3 cut(s) 1244, 1402, 2695
Bse8I GATNNNNATC 3 cut(s) 498, 1116, 2583
BseAI TCCGGA 2 cut(s) 502, 1435
BseBI CCWGG 4 cut(s) 1259, 1889, 2319, 2789
BseCI ATCGAT 1 cut(s) 1441
BseDI CCNNGG 7 cut(s) 96, 305, 897, 1386, 1419, 2312, 2787
BseGI GGATG 6 cut(s) 300, 687, 1041, 1315, 1411, 1612
BseJI GATNNNNATC 3 cut(s) 498, 1116, 2583
BseLI CCNNNNNNNGG 9 cut(s) 306, 515, 1435, 2076, 2318, 2405, 2652, 2760, 2761
BseMI GCAATG 3 cut(s) 1244, 1402, 2695
BseMII CTCAG 3 cut(s) 1470, 1789, 2649
BseNI ACTGG 7 cut(s) 53, 1222, 1620, 1841, 2020, 2184, 2399
BseRI GAGGAG 9 cut(s) 100, 130, 133, 187, 1131, 1408, 2448, 2481, 2523
BseSI GKGCMC 1 cut(s) 2841
BseX3I CGGCCG 2 cut(s) 426, 588
BseXI GCAGC 5 cut(s) 210, 1134, 1395, 1852, 1879
Bsh1236I CGCG 2 cut(s) 302, 665
Bsh1285I CGRYCG 4 cut(s) 82, 429, 591, 663
BshFI GGCC 8 cut(s) 57, 299, 356, 428, 590, 1887, 1965, 2317
BshNI GGYRCC 3 cut(s) 994, 2321, 2836
BshTI ACCGGT 1 cut(s) 2012
BshVI ATCGAT 1 cut(s) 1441
BsiEI CGRYCG 4 cut(s) 82, 429, 591, 663
BsiHKAI GWGCWC 2 cut(s) 1589, 2255
BsiHKCI CYCGRG 3 cut(s) 96, 453, 2226
BslFI GGGAC 4 cut(s) 65, 79, 1411, 1823
BslI CCNNNNNNNGG 9 cut(s) 306, 515, 1435, 2076, 2318, 2405, 2652, 2760, 2761
BsmAI GTCTC 3 cut(s) 199, 631, 2430
BsmBI CGTCTC 1 cut(s) 199
BsmFI GGGAC 4 cut(s) 65, 79, 1411, 1823
BsmI GAATGC 1 cut(s) 2359
BsnI GGCC 8 cut(s) 57, 299, 356, 428, 590, 1887, 1965, 2317
BsoBI CYCGRG 3 cut(s) 96, 453, 2226
Bsp119I TTCGAA 3 cut(s) 398, 524, 1593
Bsp1286I GDGCHC 4 cut(s) 1283, 1589, 2255, 2841
Bsp13I TCCGGA 2 cut(s) 502, 1435
Bsp1407I TGTACA 2 cut(s) 1929, 2392
Bsp19I CCATGG 2 cut(s) 1386, 2312
BspANI GGCC 8 cut(s) 57, 299, 356, 428, 590, 1887, 1965, 2317
BspCNI CTCAG 3 cut(s) 1471, 1788, 2648
BspDI ATCGAT 1 cut(s) 1441
BspEI TCCGGA 2 cut(s) 502, 1435
BspFNI CGCG 2 cut(s) 302, 665
BspHI TCATGA 3 cut(s) 1273, 1849, 2272
BspMI ACCTGC 1 cut(s) 601
BspPI GGATC 7 cut(s) 488, 494, 1125, 1208, 1446, 2579, 2592
BspQI GCTCTTC 2 cut(s) 1513, 2214
BspT104I TTCGAA 3 cut(s) 398, 524, 1593
BspT107I GGYRCC 3 cut(s) 994, 2321, 2836
BsrDI GCAATG 3 cut(s) 1244, 1402, 2695
BsrFI RCCGGY 3 cut(s) 931, 997, 2012
BsrGI TGTACA 2 cut(s) 1929, 2392
BsrI ACTGG 7 cut(s) 53, 1222, 1620, 1841, 2020, 2184, 2399
BssAI RCCGGY 3 cut(s) 931, 997, 2012
BssECI CCNNGG 7 cut(s) 96, 305, 897, 1386, 1419, 2312, 2787
BssNI GRCGYC 4 cut(s) 213, 388, 417, 698
BssT1I CCWWGG 2 cut(s) 1386, 2312
Bst2UI CCWGG 4 cut(s) 1259, 1889, 2319, 2789
Bst6I CTCTTC 5 cut(s) 23, 1513, 1819, 2214, 2466
BstACI GRCGYC 4 cut(s) 213, 388, 417, 698
BstAUI TGTACA 2 cut(s) 1929, 2392
BstBI TTCGAA 3 cut(s) 398, 524, 1593
BstC8I GCNNGC 4 cut(s) 228, 329, 1870, 1907
BstDEI CTNAG 6 cut(s) 1454, 1479, 1775, 2367, 2525, 2635
BstDSI CCRYGG 3 cut(s) 897, 1386, 2312
BstEII GGTNACC 3 cut(s) 1690, 1858, 2333
BstENI CCTNNNNNAGG 1 cut(s) 2650
BstF5I GGATG 6 cut(s) 300, 687, 1041, 1315, 1411, 1612
BstFNI CGCG 2 cut(s) 302, 665
BstH2I RGCGCY 2 cut(s) 485, 750
BstHHI GCGC 3 cut(s) 304, 484, 749
BstMAI GTCTC 3 cut(s) 199, 631, 2430
BstMCI CGRYCG 4 cut(s) 82, 429, 591, 663
BstMWI GCNNNNNNNGC 9 cut(s) 236, 337, 387, 447, 544, 587, 665, 1144, 2221
BstNI CCWGG 4 cut(s) 1259, 1889, 2319, 2789
BstNSI RCATGY 1 cut(s) 1872
BstPAI GACNNNNGTC 1 cut(s) 168
BstPI GGTNACC 3 cut(s) 1690, 1858, 2333
BstSLI GKGCMC 1 cut(s) 2841
BstUI CGCG 2 cut(s) 302, 665
BstV1I GCAGC 5 cut(s) 210, 1134, 1395, 1852, 1879
BstV2I GAAGAC 2 cut(s) 78, 2700
BstX2I RGATCY 1 cut(s) 2584
BstYI RGATCY 1 cut(s) 2584
BstZI CGGCCG 2 cut(s) 426, 588
Bsu15I ATCGAT 1 cut(s) 1441
BsuI GTATCC 1 cut(s) 2817
BsuRI GGCC 8 cut(s) 57, 299, 356, 428, 590, 1887, 1965, 2317
BsuTUI ATCGAT 1 cut(s) 1441
BtgI CCRYGG 3 cut(s) 897, 1386, 2312
BtrI CACGTC 1 cut(s) 712
BtsCI GGATG 6 cut(s) 300, 687, 1041, 1315, 1411, 1612
BtsIMutI CAGTG 4 cut(s) 621, 1771, 2191, 2291
BveI ACCTGC 1 cut(s) 601
Cac8I GCNNGC 4 cut(s) 228, 329, 1870, 1907
CaiI CAGNNNCTG 1 cut(s) 2411
CciI TCATGA 3 cut(s) 1273, 1849, 2272
CfoI GCGC 3 cut(s) 304, 484, 749
Cfr10I RCCGGY 3 cut(s) 931, 997, 2012
Cfr13I GGNCC 7 cut(s) 555, 1156, 1290, 1618, 1629, 2718, 2858
Cfr9I CCCGGG 1 cut(s) 96
ClaI ATCGAT 1 cut(s) 1441
CseI GACGC 5 cut(s) 181, 396, 406, 706, 2136
CspAI ACCGGT 1 cut(s) 2012
DdeI CTNAG 6 cut(s) 1454, 1479, 1775, 2367, 2525, 2635
DraI TTTAAA 1 cut(s) 1707
DrdI GACNNNNNNGTC 2 cut(s) 649, 1222
DseDI GACNNNNNNGTC 2 cut(s) 649, 1222
EaeI YGGCCR 5 cut(s) 297, 354, 426, 588, 1885
EagI CGGCCG 2 cut(s) 426, 588
Eam1104I CTCTTC 5 cut(s) 23, 1513, 1819, 2214, 2466
EarI CTCTTC 5 cut(s) 23, 1513, 1819, 2214, 2466
EciI GGCGGA 2 cut(s) 103, 226
EclXI CGGCCG 2 cut(s) 426, 588
Eco130I CCWWGG 2 cut(s) 1386, 2312
Eco24I GRGCYC 1 cut(s) 1283
Eco47I GGWCC 7 cut(s) 555, 1156, 1290, 1618, 1629, 2718, 2858
Eco52I CGGCCG 2 cut(s) 426, 588
Eco57I CTGAAG 2 cut(s) 367, 1482
Eco88I CYCGRG 3 cut(s) 96, 453, 2226
Eco91I GGTNACC 3 cut(s) 1690, 1858, 2333
EcoNI CCTNNNNNAGG 1 cut(s) 2650
EcoO109I RGGNCCY 1 cut(s) 2858
EcoO65I GGTNACC 3 cut(s) 1690, 1858, 2333
EcoRI GAATTC 2 cut(s) 526, 1553
EcoRII CCWGG 4 cut(s) 1257, 1887, 2317, 2787
EcoT14I CCWWGG 2 cut(s) 1386, 2312
EcoT22I ATGCAT 1 cut(s) 2695
EcoT38I GRGCYC 1 cut(s) 1283
ErhI CCWWGG 2 cut(s) 1386, 2312
Esp3I CGTCTC 1 cut(s) 199
FalI AAGNNNNNCTT 2 cut(s) 1289, 1321
FaqI GGGAC 4 cut(s) 65, 79, 1411, 1823
FauI CCCGC 2 cut(s) 1413, 2352
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 758
FokI GGATG 6 cut(s) 307, 694, 1048, 1322, 1398, 1619
FriOI GRGCYC 1 cut(s) 1283
FspBI CTAG 9 cut(s) 672, 678, 735, 1314, 1326, 1512, 2484, 2496, 2866
GlaI GCGC 3 cut(s) 303, 483, 748
GsuI CTGGAG 2 cut(s) 1353, 1863
HaeII RGCGCY 2 cut(s) 485, 750
HaeIII GGCC 8 cut(s) 57, 299, 356, 428, 590, 1887, 1965, 2317
HgaI GACGC 5 cut(s) 181, 396, 406, 706, 2136
HhaI GCGC 3 cut(s) 304, 484, 749
Hin1I GRCGYC 4 cut(s) 213, 388, 417, 698
Hin6I GCGC 3 cut(s) 302, 482, 747
HinP1I GCGC 3 cut(s) 302, 482, 747
HincII GTYRAC 2 cut(s) 759, 967
HindII GTYRAC 2 cut(s) 759, 967
HindIII AAGCTT 4 cut(s) 579, 1464, 1796, 2757
Hpy166II GTNNAC 8 cut(s) 759, 768, 832, 901, 967, 1100, 1834, 2086
Hpy8I GTNNAC 8 cut(s) 759, 768, 832, 901, 967, 1100, 1834, 2086
HpyCH4IV ACGT 5 cut(s) 35, 213, 650, 711, 2383
HpyF10VI GCNNNNNNNGC 9 cut(s) 236, 337, 387, 447, 544, 587, 665, 1144, 2221
HpyF3I CTNAG 6 cut(s) 1454, 1479, 1775, 2367, 2525, 2635
HpySE526I ACGT 5 cut(s) 35, 213, 650, 711, 2383
Hsp92I GRCGYC 4 cut(s) 213, 388, 417, 698
HspAI GCGC 3 cut(s) 302, 482, 747
Kpn2I TCCGGA 2 cut(s) 502, 1435
KpnI GGTACC 1 cut(s) 998
Ksp22I TGATCA 1 cut(s) 3
LguI GCTCTTC 2 cut(s) 1513, 2214
LmnI GCTCC 7 cut(s) 10, 505, 673, 1004, 1234, 1787, 2144
Lsp1109I GCAGC 5 cut(s) 210, 1134, 1395, 1852, 1879
LweI GCATC 8 cut(s) 534, 939, 1300, 1420, 1597, 1780, 2423, 2702
MaeI CTAG 9 cut(s) 672, 678, 735, 1314, 1326, 1512, 2484, 2496, 2866
MaeII ACGT 5 cut(s) 35, 213, 650, 711, 2383
MflI RGATCY 1 cut(s) 2584
MhlI GDGCHC 4 cut(s) 1283, 1589, 2255, 2841
MlsI TGGCCA 2 cut(s) 356, 1887
MluNI TGGCCA 2 cut(s) 356, 1887
MlyI GAGTC 4 cut(s) 101, 521, 2557, 2633
MmeI TCCRAC 2 cut(s) 171, 836
Mox20I TGGCCA 2 cut(s) 356, 1887
Mph1103I ATGCAT 1 cut(s) 2695
MroI TCCGGA 2 cut(s) 502, 1435
MroXI GAANNNNTTC 2 cut(s) 2280, 2608
MscI TGGCCA 2 cut(s) 356, 1887
MseI TTAA 5 cut(s) 798, 1706, 1952, 1977, 2034
MslI CAYNNNNRTG 3 cut(s) 293, 1605, 2202
Msp20I TGGCCA 2 cut(s) 356, 1887
MspA1I CMGCKG 6 cut(s) 1147, 1408, 1779, 1865, 2507, 2549
Mva1269I GAATGC 1 cut(s) 2359
MvaI CCWGG 4 cut(s) 1259, 1889, 2319, 2789
MvnI CGCG 2 cut(s) 302, 665
MwoI GCNNNNNNNGC 9 cut(s) 236, 337, 387, 447, 544, 587, 665, 1144, 2221
NciI CCSGG 6 cut(s) 97, 98, 306, 979, 2071, 2841
NcoI CCATGG 2 cut(s) 1386, 2312
NmeAIII GCCGAG 2 cut(s) 268, 566
NmuCI GTSAC 7 cut(s) 273, 1690, 1858, 2053, 2098, 2333, 2776
NsiI ATGCAT 1 cut(s) 2695
NspI RCATGY 1 cut(s) 1872
NspV TTCGAA 3 cut(s) 398, 524, 1593
PaeI GCATGC 1 cut(s) 1872
PaeR7I CTCGAG 1 cut(s) 2226
PagI TCATGA 3 cut(s) 1273, 1849, 2272
PciSI GCTCTTC 2 cut(s) 1513, 2214
PcsI WCGNNNNNNNCGW 7 cut(s) 60, 72, 189, 266, 521, 536, 656
PctI GAATGC 1 cut(s) 2359
PdmI GAANNNNTTC 2 cut(s) 2280, 2608
PfeI GAWTC 9 cut(s) 105, 1264, 1993, 2066, 2192, 2448, 2609, 2666, 2738
PflFI GACNNNGTC 3 cut(s) 374, 1343, 1538
PflMI CCANNNNNTGG 1 cut(s) 2318
PfoI TCCNGGA 1 cut(s) 2069
PinAI ACCGGT 1 cut(s) 2012
Ple19I CGATCG 1 cut(s) 663
PleI GAGTC 4 cut(s) 100, 520, 2557, 2632
PpsI GAGTC 4 cut(s) 100, 520, 2557, 2632
PpuMI RGGWCCY 1 cut(s) 2858
PshAI GACNNNNGTC 1 cut(s) 168
PshBI ATTAAT 1 cut(s) 2034
Psp1406I AACGTT 1 cut(s) 2383
Psp5II RGGWCCY 1 cut(s) 2858
Psp6I CCWGG 4 cut(s) 1257, 1887, 2317, 2787
PspEI GGTNACC 3 cut(s) 1690, 1858, 2333
PspGI CCWGG 4 cut(s) 1257, 1887, 2317, 2787
PspPI GGNCC 7 cut(s) 555, 1156, 1290, 1618, 1629, 2718, 2858
PspPPI RGGWCCY 1 cut(s) 2858
PspXI VCTCGAGB 1 cut(s) 2226
PsrI GAACNNNNNNTAC 2 cut(s) 1347, 1379
PstNI CAGNNNCTG 1 cut(s) 2411
PsuI RGATCY 1 cut(s) 2584
PsyI GACNNNGTC 3 cut(s) 374, 1343, 1538
PvuI CGATCG 1 cut(s) 663
RgaI GCGATCGC 1 cut(s) 663
RseI CAYNNNNRTG 3 cut(s) 293, 1605, 2202
SalI GTCGAC 1 cut(s) 757
SapI GCTCTTC 2 cut(s) 1513, 2214
SaqAI TTAA 5 cut(s) 798, 1706, 1952, 1977, 2034
Sau96I GGNCC 7 cut(s) 555, 1156, 1290, 1618, 1629, 2718, 2858
ScaI AGTACT 3 cut(s) 1614, 2488, 2530
SchI GAGTC 4 cut(s) 101, 521, 2557, 2633
SduI GDGCHC 4 cut(s) 1283, 1589, 2255, 2841
SfaAI GCGATCGC 1 cut(s) 663
SfaNI GCATC 8 cut(s) 534, 939, 1300, 1420, 1597, 1780, 2423, 2702
Sfr274I CTCGAG 1 cut(s) 2226
SfuI TTCGAA 3 cut(s) 398, 524, 1593
SgfI GCGATCGC 1 cut(s) 663
SgrAI CRCCGGYG 1 cut(s) 931
SinI GGWCC 7 cut(s) 555, 1156, 1290, 1618, 1629, 2718, 2858
SlaI CTCGAG 1 cut(s) 2226
SmaI CCCGGG 1 cut(s) 98
SmiMI CAYNNNNRTG 3 cut(s) 293, 1605, 2202
SmlI CTYRAG 2 cut(s) 2226, 2424
SmoI CTYRAG 2 cut(s) 2226, 2424
SphI GCATGC 1 cut(s) 1872
SspI AATATT 1 cut(s) 2279
SspMI CTAG 9 cut(s) 672, 678, 735, 1314, 1326, 1512, 2484, 2496, 2866
StyI CCWWGG 2 cut(s) 1386, 2312
TaiI ACGT 5 cut(s) 38, 216, 653, 714, 2386
TatI WGTACW 7 cut(s) 1099, 1612, 1929, 2169, 2392, 2486, 2528
TfiI GAWTC 9 cut(s) 105, 1264, 1993, 2066, 2192, 2448, 2609, 2666, 2738
Tru1I TTAA 5 cut(s) 798, 1706, 1952, 1977, 2034
Tru9I TTAA 5 cut(s) 798, 1706, 1952, 1977, 2034
TscAI CASTG 4 cut(s) 628, 1771, 2191, 2291
TseFI GTSAC 7 cut(s) 273, 1690, 1858, 2053, 2098, 2333, 2776
TseI GCWGC 5 cut(s) 223, 1147, 1408, 1865, 1892
Tsp45I GTSAC 7 cut(s) 273, 1690, 1858, 2053, 2098, 2333, 2776
TspDTI ATGAA 8 cut(s) 59, 814, 1340, 1641, 1838, 1866, 2289, 2751
TspGWI ACGGA 5 cut(s) 190, 776, 907, 2445, 2621
TspMI CCCGGG 1 cut(s) 96
TspRI CASTG 4 cut(s) 628, 1771, 2191, 2291
Tth111I GACNNNGTC 3 cut(s) 374, 1343, 1538
Van91I CCANNNNNTGG 1 cut(s) 2318
VpaK11BI GGWCC 7 cut(s) 555, 1156, 1290, 1618, 1629, 2718, 2858
VspI ATTAAT 1 cut(s) 2034
XagI CCTNNNNNAGG 1 cut(s) 2650
XapI RAATTY 4 cut(s) 526, 1553, 2595, 2815
XceI RCATGY 1 cut(s) 1872
XhoI CTCGAG 1 cut(s) 2226
XmaI CCCGGG 1 cut(s) 96
XmiI GTMKAC 1 cut(s) 758
XmnI GAANNNNTTC 2 cut(s) 2280, 2608
XspI CTAG 9 cut(s) 672, 678, 735, 1314, 1326, 1512, 2484, 2496, 2866
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 3 cut(s) 1614, 2488, 2530
Zsp2I ATGCAT 1 cut(s) 2695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.