AT5G03450

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011045
Physical Location & Seq
Reverse (-)
858735 .. 862345
3611 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03450.1

Sequence Viewer

Length: 1893 bp
ATGCCGCCTCGCCGGAGTTACCCCTTCAGTGGTCACGGAGGTACAATTGCACGATTCATTGAATTTGGGGAAGCATTTGGACACTATGGAGAAGAAGAAGACGAAGAAGAGGAAGACGAAGAGGAAGCATATGTCGACTATGGTGAAGTACAAGAGGAAGACGAGGAGGAAGAGGAGGAAGAAGATTCCGAACGCCAAACCAGGGAATACATCGATGTCCTAGACTCACCTGTTAGGGTTTCACAGAACGAGAGTGAAGGGATAGAGAATCAGAATAGGTCACAAGGTGTTTGTTCATCGTCTTCCGGATCGCAGGAGGATGTCGAATGGAAACAAGGTGACACTGAGGGATTGTCTTGTTCTATCTGTATGGAAGTTTGGACCAGCGGTGGCCAACACCAAGTCTGTTGTCTTCCCTGTGGACACTTGTATGGATATTCCTGCATCAACAAGTGGTTTCAGCAGCGACGAAGTGGAGGGAAGTGTCCCCTGTGCAACAAAATATGTAGCTTGAGAGATGTCCGGAAAATCTATGCATCACGGGTTGCTGCAGTAGATGATGAAGCACACAAGAGAATCTTATTTCTGGAGGCCAAGTTAAGGTCAATTGAACAGAAGACTGCGAGTTGGAGTAACAAAGAAGTTCAATGGAAAATAACTGAAGCTGAGCTACGATTAGAAGTTACTAAGCTGAAGAAGAAAATAGCTAATATGGAAAGTACGACTAAGGGTTCGCAAAGCCAGTCTAATGTGGCTTCTCAGGAGAAATATATACCTGCTCATAATATCTACCAAGAGCATCATGGACAGTCACCTTCTTGCAGCTTCAGACATCAGGGAGATCTGCTAATAAACGGTGGCCGTATATTTGACATAGATGGTGGTAGACAAATATTATTATATGCCCGTAGGCTCTCAGGCGTTGGTGGAACGTTTGTGCTTACGCAAATGAGCCTACATTCGCCTTGTGAGATTGATGATATTTTGTTGCCTCGTACCACTAGAGCAATTAAAGATCTTCACCTTTCCCCTCACAATAATGGGCTTGCAGTATTTGGGTCTCTAGGAAAAAAATTATCTGTTATAAGCTTGGAGAGCCACAATACTGTCTTATCTTATGATCTACCGGCTGCACCTTGGTCTTGCTCGTGGGATCTCAAAAGCTCTCACCATATTTATGCTGGACTACAAAATGGAATGGTTTTGATATTTGACATGCGTCAAACCACGGGACCTTTCGCATCGATGACAGGCTTAACAACCAATCCAGTTCATACTGTCCATCATCTCTCTGGCAATTCCACTCCAACTTCTGATGTCCGTACCCTCTTATCTGCTTCTTCCATCGGGCTGTGTCAGTGGAACATTAGTGGTAGCGAGGGAAGCCCATCATTAGTTTCGGAAACGGGAAACCCAGGTGTATGCATATCCTCCTCGTACTGTCCCCGCAGCAATCATATAGTAGCTTCTTATAGACCAAGAGTTGGATCATCTGATGATACAGTCCCGACTCAGCCCTCATTGACTCAAACAGGAGTCAGTAATAGTAATAGTAATGGTGTAGATGGATTTCATGTTTGTCACAAGAGAAGAGGTGTTGATTCGTATTACCAGAAACTATCCTCCACACAAGCTATTGTAGATCCCATCCGTTTGCCAAGAACAGCGATTATAGACTTTGGTGGGGAAAGGAAACAACTTTTTGCATCTTGTGACGAGTCCACTCGGGAGCTCATCTTGCAAGATCCTTCGACTTTTACTATTTCTCAACGGTTGCCATTGTCAAGTCATCTTCCGCTTCAAGATGTGAAATACTGTCACGTCAATGGTTCGGATCTGCTCGGTCTCTTAACGGATGATAGATTGCAGATTCTTAGGAACGAATCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

630

Amino Acids

69.87

Weight (kDa)

5.47

Isoelectric Point (pI)

66.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 119 - 166 6.5e-09 Ring finger domain
WD40_RFWD3 PF23419 334 - 624 1.7e-27 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03450 AT5G03750
fragaria_vesca FvH4_6g16560 FvH4_6g16560
malus_domestica MD12G1109200.v1.1
prunus_persica Prupe.7G127800_v2.0.a1
pyrus_communis pycom12g10940
rosa_chinensis RchiOBHm_Chr3g0470481
rosa_laevigata RLG00000024263
rosa_multiflora Rmu_sc0002115.1_g000004 Rmu_sc0003381.1_g000011
rosa_roxburghii Rroxscaffold_4G00302270 Rroxscaffold_6G00410770
rosa_rugosa Rorug03G0109400 Rorug03G0109500
rosa_samantha Rh3AG159800 Rh3BG184300 Rh3CG174800 Rh3DG190600
rosa_wichuraiana Rw3G015120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1085
Acc36I ACCTGC 1 cut(s) 784
AccB7I CCANNNNNTGG 1 cut(s) 1484
AccI GTMKAC 2 cut(s) 135, 886
AccIII TCCGGA 2 cut(s) 305, 522
AciI CCGC 4 cut(s) 5, 387, 1447, 1796
AclI AACGTT 1 cut(s) 932
AclWI GGATC 6 cut(s) 316, 1161, 1495, 1637, 1739, 1842
AcoI YGGCCR 2 cut(s) 391, 859
AcsI RAATTY 1 cut(s) 62
AcuI CTGAAG 4 cut(s) 10, 681, 713, 811
AdeI CACNNNGTG 1 cut(s) 287
AfaI GTAC 6 cut(s) 43, 150, 721, 997, 1324, 1439
AfiI CCNNNNNNNGG 4 cut(s) 29, 202, 600, 1484
AgsI TTSAA 4 cut(s) 62, 611, 647, 1802
AjiI CACGTC 1 cut(s) 1822
AjnI CCWGG 2 cut(s) 200, 1414
Alw21I GWGCWC 1 cut(s) 1734
Alw26I GTCTC 2 cut(s) 1065, 1850
AlwI GGATC 6 cut(s) 316, 1161, 1495, 1637, 1739, 1842
Ama87I CYCGRG 1 cut(s) 1725
Aor13HI TCCGGA 2 cut(s) 305, 522
AoxI GGCC 3 cut(s) 391, 591, 859
ApeKI GCWGC 5 cut(s) 463, 548, 822, 1130, 1449
ApoI RAATTY 1 cut(s) 62
ArsI GACNNNNNNTTYG 2 cut(s) 715, 747
AspS9I GGNCC 2 cut(s) 381, 1232
AsuHPI GGTGA 6 cut(s) 155, 219, 350, 804, 1013, 1160
AvaI CYCGRG 1 cut(s) 1725
AvaII GGWCC 2 cut(s) 381, 1232
BalI TGGCCA 1 cut(s) 393
BanII GRGCYC 1 cut(s) 1734
BarI GAAGNNNNNNTAC 2 cut(s) 654, 686
BauI CACGAG 1 cut(s) 1147
BbsI GAAGAC 6 cut(s) 105, 120, 165, 294, 404, 623
Bbv12I GWGCWC 1 cut(s) 1734
BbvI GCAGC 5 cut(s) 475, 535, 834, 1117, 1461
BccI CCATC 6 cut(s) 872, 1290, 1352, 1396, 1559, 1655
BceAI ACGGC 1 cut(s) 846
BciT130I CCWGG 2 cut(s) 202, 1416
BcoDI GTCTC 2 cut(s) 1065, 1850
BfaI CTAG 3 cut(s) 221, 1002, 1064
BfmI CTRYAG 1 cut(s) 549
BfuAI ACCTGC 1 cut(s) 784
BglII AGATCT 2 cut(s) 841, 1015
BisI GCNGC 6 cut(s) 5, 464, 549, 823, 1131, 1450
BlpI GCTNAGC 1 cut(s) 666
BlsI GCNGC 6 cut(s) 6, 465, 550, 824, 1132, 1451
Bme1390I CCNGG 2 cut(s) 202, 1416
Bme18I GGWCC 2 cut(s) 381, 1232
BmeT110I CYCGRG 1 cut(s) 1725
BmgBI CACGTC 1 cut(s) 1822
BmgT120I GGNCC 2 cut(s) 381, 1232
BmiI GGNNCC 1 cut(s) 1233
BmrFI CCNGG 2 cut(s) 202, 1416
BmsI GCATC 5 cut(s) 453, 545, 808, 1250, 1715
BoxI GACNNNNGTC 1 cut(s) 1218
BpiI GAAGAC 6 cut(s) 105, 120, 165, 294, 404, 623
BpmI CTGGAG 1 cut(s) 608
Bpu1102I GCTNAGC 1 cut(s) 666
BpuEI CTTGAG 1 cut(s) 532
Bsa29I ATCGAT 2 cut(s) 213, 1244
BsaI GGTCTC 2 cut(s) 1065, 1850
BsaJI CCNNGG 4 cut(s) 201, 1136, 1227, 1414
BsaWI WCCGGW 2 cut(s) 305, 522
BsaXI ACNNNNNCTCC 2 cut(s) 581, 611
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 202, 600, 1484
Bse118I RCCGGY 1 cut(s) 1126
Bse1I ACTGG 2 cut(s) 742, 1268
BseAI TCCGGA 2 cut(s) 305, 522
BseBI CCWGG 2 cut(s) 202, 1416
BseCI ATCGAT 2 cut(s) 213, 1244
BseDI CCNNGG 4 cut(s) 201, 1136, 1227, 1414
BseGI GGATG 3 cut(s) 325, 1647, 1861
BseLI CCNNNNNNNGG 4 cut(s) 29, 202, 600, 1484
BseMII CTCAG 5 cut(s) 336, 657, 773, 930, 1526
BseNI ACTGG 2 cut(s) 742, 1268
BseRI GAGGAG 3 cut(s) 179, 188, 1423
BseXI GCAGC 5 cut(s) 475, 535, 834, 1117, 1461
BsgI GTGCAG 1 cut(s) 1116
BshFI GGCC 3 cut(s) 393, 593, 861
BshVI ATCGAT 2 cut(s) 213, 1244
BsiHKAI GWGCWC 1 cut(s) 1734
BsiHKCI CYCGRG 1 cut(s) 1725
BsiSI CCGG 4 cut(s) 13, 306, 523, 1127
BslFI GGGAC 4 cut(s) 471, 1245, 1428, 1490
BslI CCNNNNNNNGG 4 cut(s) 29, 202, 600, 1484
BsmAI GTCTC 2 cut(s) 1065, 1850
BsmFI GGGAC 4 cut(s) 471, 1245, 1428, 1490
BsnI GGCC 3 cut(s) 393, 593, 861
Bso31I GGTCTC 2 cut(s) 1065, 1850
BsoBI CYCGRG 1 cut(s) 1725
Bsp1286I GDGCHC 1 cut(s) 1734
Bsp13I TCCGGA 2 cut(s) 305, 522
Bsp143I GATC 9 cut(s) 308, 841, 1015, 1120, 1153, 1487, 1642, 1744, 1834
Bsp1720I GCTNAGC 1 cut(s) 666
BspACI CCGC 4 cut(s) 5, 387, 1447, 1796
BspANI GGCC 3 cut(s) 393, 593, 861
BspCNI CTCAG 5 cut(s) 337, 658, 772, 929, 1525
BspDI ATCGAT 2 cut(s) 213, 1244
BspEI TCCGGA 2 cut(s) 305, 522
BspLI GGNNCC 1 cut(s) 1233
BspMAI CTGCAG 1 cut(s) 553
BspMI ACCTGC 1 cut(s) 784
BspPI GGATC 6 cut(s) 316, 1161, 1495, 1637, 1739, 1842
BspTNI GGTCTC 2 cut(s) 1065, 1850
BsrFI RCCGGY 1 cut(s) 1126
BsrI ACTGG 2 cut(s) 742, 1268
BssAI RCCGGY 1 cut(s) 1126
BssECI CCNNGG 4 cut(s) 201, 1136, 1227, 1414
BssMI GATC 9 cut(s) 308, 841, 1015, 1120, 1153, 1487, 1642, 1744, 1834
BssSI CACGAG 1 cut(s) 1147
BssT1I CCWWGG 1 cut(s) 1136
Bst2BI CACGAG 1 cut(s) 1147
Bst2UI CCWGG 2 cut(s) 202, 1416
Bst4CI ACNGT 8 cut(s) 810, 857, 1108, 1279, 1442, 1504, 1773, 1817
Bst6I CTCTTC 4 cut(s) 102, 114, 165, 1585
BstC8I GCNNGC 1 cut(s) 1047
BstDEI CTNAG 8 cut(s) 345, 666, 687, 726, 759, 916, 1512, 1874
BstDSI CCRYGG 1 cut(s) 1227
BstF5I GGATG 3 cut(s) 325, 1647, 1861
BstKTI GATC 9 cut(s) 311, 844, 1018, 1123, 1156, 1490, 1645, 1747, 1837
BstMAI GTCTC 2 cut(s) 1065, 1850
BstMBI GATC 9 cut(s) 308, 841, 1015, 1120, 1153, 1487, 1642, 1744, 1834
BstMWI GCNNNNNNNGC 3 cut(s) 1095, 1383, 1738
BstNI CCWGG 2 cut(s) 202, 1416
BstNSI RCATGY 1 cut(s) 1219
BstPAI GACNNNNGTC 1 cut(s) 1218
BstSCI CCNGG 2 cut(s) 200, 1414
BstSFI CTRYAG 1 cut(s) 549
BstV1I GCAGC 5 cut(s) 475, 535, 834, 1117, 1461
BstV2I GAAGAC 6 cut(s) 105, 120, 165, 294, 404, 623
BstX2I RGATCY 6 cut(s) 841, 1015, 1153, 1642, 1744, 1834
BstYI RGATCY 6 cut(s) 841, 1015, 1153, 1642, 1744, 1834
Bsu15I ATCGAT 2 cut(s) 213, 1244
BsuRI GGCC 3 cut(s) 393, 593, 861
BsuTUI ATCGAT 2 cut(s) 213, 1244
BtgI CCRYGG 1 cut(s) 1227
BtrI CACGTC 1 cut(s) 1822
BtsCI GGATG 3 cut(s) 325, 1647, 1861
BtsIMutI CAGTG 3 cut(s) 34, 342, 1364
BveI ACCTGC 1 cut(s) 784
Cac8I GCNNGC 1 cut(s) 1047
Cfr10I RCCGGY 1 cut(s) 1126
Cfr13I GGNCC 2 cut(s) 381, 1232
ClaI ATCGAT 2 cut(s) 213, 1244
CseI GACGC 1 cut(s) 1208
Csp6I GTAC 6 cut(s) 42, 149, 720, 996, 1323, 1438
CviAII CATG 4 cut(s) 803, 1216, 1574, 1890
CviQI GTAC 6 cut(s) 42, 149, 720, 996, 1323, 1438
DdeI CTNAG 8 cut(s) 345, 666, 687, 726, 759, 916, 1512, 1874
DpnI GATC 9 cut(s) 310, 843, 1017, 1122, 1155, 1489, 1644, 1746, 1836
DpnII GATC 9 cut(s) 308, 841, 1015, 1120, 1153, 1487, 1642, 1744, 1834
DraIII CACNNNGTG 1 cut(s) 287
EaeI YGGCCR 2 cut(s) 391, 859
Eam1104I CTCTTC 4 cut(s) 102, 114, 165, 1585
EarI CTCTTC 4 cut(s) 102, 114, 165, 1585
Ecl136II GAGCTC 1 cut(s) 1732
Eco130I CCWWGG 1 cut(s) 1136
Eco24I GRGCYC 1 cut(s) 1734
Eco31I GGTCTC 2 cut(s) 1065, 1850
Eco47I GGWCC 2 cut(s) 381, 1232
Eco53kI GAGCTC 1 cut(s) 1732
Eco57I CTGAAG 4 cut(s) 10, 681, 713, 811
Eco88I CYCGRG 1 cut(s) 1725
EcoICRI GAGCTC 1 cut(s) 1732
EcoO109I RGGNCCY 1 cut(s) 1232
EcoRII CCWGG 2 cut(s) 200, 1414
EcoT14I CCWWGG 1 cut(s) 1136
EcoT22I ATGCAT 2 cut(s) 538, 1427
EcoT38I GRGCYC 1 cut(s) 1734
ErhI CCWWGG 1 cut(s) 1136
FaeI CATG 4 cut(s) 806, 1219, 1577, 1893
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FaqI GGGAC 4 cut(s) 471, 1245, 1428, 1490
FatI CATG 4 cut(s) 802, 1215, 1573, 1889
FauI CCCGC 1 cut(s) 1454
FauNDI CATATG 1 cut(s) 130
FblI GTMKAC 2 cut(s) 135, 886
Fnu4HI GCNGC 6 cut(s) 5, 464, 549, 823, 1131, 1450
FokI GGATG 3 cut(s) 332, 1634, 1868
FriOI GRGCYC 1 cut(s) 1734
Fsp4HI GCNGC 6 cut(s) 5, 464, 549, 823, 1131, 1450
FspBI CTAG 3 cut(s) 221, 1002, 1064
GluI GCNGC 6 cut(s) 5, 464, 549, 823, 1131, 1450
GsuI CTGGAG 1 cut(s) 608
HaeIII GGCC 3 cut(s) 393, 593, 861
HapII CCGG 4 cut(s) 13, 306, 523, 1127
HgaI GACGC 1 cut(s) 1208
Hin1II CATG 4 cut(s) 806, 1219, 1577, 1893
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 1087
HpaII CCGG 4 cut(s) 13, 306, 523, 1127
HphI GGTGA 6 cut(s) 155, 219, 350, 804, 1013, 1160
Hpy166II GTNNAC 4 cut(s) 136, 422, 887, 1722
Hpy188I TCNGA 7 cut(s) 190, 273, 830, 1315, 1402, 1495, 1834
Hpy188III TCNNGA 7 cut(s) 306, 523, 587, 761, 1507, 1727, 1802
Hpy8I GTNNAC 4 cut(s) 136, 422, 887, 1722
Hpy99I CGWCG 1 cut(s) 471
HpyAV CCTTC 4 cut(s) 34, 251, 825, 1758
HpyCH4III ACNGT 8 cut(s) 810, 857, 1108, 1279, 1442, 1504, 1773, 1817
HpyCH4IV ACGT 2 cut(s) 932, 1821
HpyF10VI GCNNNNNNNGC 3 cut(s) 1095, 1383, 1738
HpyF3I CTNAG 8 cut(s) 345, 666, 687, 726, 759, 916, 1512, 1874
HpySE526I ACGT 2 cut(s) 932, 1821
Hsp92II CATG 4 cut(s) 806, 1219, 1577, 1893
Kpn2I TCCGGA 2 cut(s) 305, 522
Kzo9I GATC 9 cut(s) 308, 841, 1015, 1120, 1153, 1487, 1642, 1744, 1834
LmnI GCTCC 1 cut(s) 1729
Lsp1109I GCAGC 5 cut(s) 475, 535, 834, 1117, 1461
LweI GCATC 5 cut(s) 453, 545, 808, 1250, 1715
MaeI CTAG 3 cut(s) 221, 1002, 1064
MaeII ACGT 2 cut(s) 932, 1821
MalI GATC 9 cut(s) 310, 843, 1017, 1122, 1155, 1489, 1644, 1746, 1836
MboI GATC 9 cut(s) 308, 841, 1015, 1120, 1153, 1487, 1642, 1744, 1834
MfeI CAATTG 2 cut(s) 45, 606
MflI RGATCY 6 cut(s) 841, 1015, 1153, 1642, 1744, 1834
MhlI GDGCHC 1 cut(s) 1734
MlsI TGGCCA 1 cut(s) 393
MluCI AATT 6 cut(s) 45, 62, 606, 1008, 1073, 1297
MluNI TGGCCA 1 cut(s) 393
MlyI GAGTC 5 cut(s) 218, 1504, 1519, 1545, 1727
MmeI TCCRAC 3 cut(s) 608, 1331, 1465
Mox20I TGGCCA 1 cut(s) 393
Mph1103I ATGCAT 2 cut(s) 538, 1427
MroI TCCGGA 2 cut(s) 305, 522
MscI TGGCCA 1 cut(s) 393
MseI TTAA 4 cut(s) 599, 1011, 1256, 1850
MslI CAYNNNNRTG 4 cut(s) 429, 1038, 1176, 1824
Msp20I TGGCCA 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 387
MspI CCGG 4 cut(s) 13, 306, 523, 1127
MspR9I CCNGG 2 cut(s) 202, 1416
MunI CAATTG 2 cut(s) 45, 606
MvaI CCWGG 2 cut(s) 202, 1416
MwoI GCNNNNNNNGC 3 cut(s) 1095, 1383, 1738
NdeI CATATG 1 cut(s) 130
NdeII GATC 9 cut(s) 308, 841, 1015, 1120, 1153, 1487, 1642, 1744, 1834
NlaIII CATG 4 cut(s) 806, 1219, 1577, 1893
NlaIV GGNNCC 1 cut(s) 1233
NmuCI GTSAC 7 cut(s) 32, 279, 338, 810, 1580, 1712, 1817
NsiI ATGCAT 2 cut(s) 538, 1427
NspI RCATGY 1 cut(s) 1219
PfeI GAWTC 7 cut(s) 54, 185, 268, 576, 1601, 1870, 1883
PflMI CCANNNNNTGG 1 cut(s) 1484
PkrI GCNGC 6 cut(s) 6, 465, 550, 824, 1132, 1451
PleI GAGTC 5 cut(s) 218, 1504, 1519, 1544, 1726
PpsI GAGTC 5 cut(s) 218, 1504, 1519, 1544, 1726
PpuMI RGGWCCY 1 cut(s) 1232
PshAI GACNNNNGTC 1 cut(s) 1218
PsiI TTATAA 1 cut(s) 1085
Psp124BI GAGCTC 1 cut(s) 1734
Psp1406I AACGTT 1 cut(s) 932
Psp5II RGGWCCY 1 cut(s) 1232
Psp6I CCWGG 2 cut(s) 200, 1414
PspGI CCWGG 2 cut(s) 200, 1414
PspN4I GGNNCC 1 cut(s) 1233
PspPI GGNCC 2 cut(s) 381, 1232
PspPPI RGGWCCY 1 cut(s) 1232
PstI CTGCAG 1 cut(s) 553
PsuI RGATCY 6 cut(s) 841, 1015, 1153, 1642, 1744, 1834
RsaI GTAC 6 cut(s) 43, 150, 721, 997, 1324, 1439
RsaNI GTAC 6 cut(s) 42, 149, 720, 996, 1323, 1438
RseI CAYNNNNRTG 4 cut(s) 429, 1038, 1176, 1824
SacI GAGCTC 1 cut(s) 1734
SalI GTCGAC 1 cut(s) 134
SaqAI TTAA 4 cut(s) 599, 1011, 1256, 1850
SatI GCNGC 6 cut(s) 5, 464, 549, 823, 1131, 1450
Sau3AI GATC 9 cut(s) 308, 841, 1015, 1120, 1153, 1487, 1642, 1744, 1834
Sau96I GGNCC 2 cut(s) 381, 1232
SchI GAGTC 5 cut(s) 218, 1504, 1519, 1545, 1727
ScrFI CCNGG 2 cut(s) 202, 1416
SduI GDGCHC 1 cut(s) 1734
SfaNI GCATC 5 cut(s) 453, 545, 808, 1250, 1715
SfcI CTRYAG 1 cut(s) 549
SinI GGWCC 2 cut(s) 381, 1232
SmiMI CAYNNNNRTG 4 cut(s) 429, 1038, 1176, 1824
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
Sse9I AATT 6 cut(s) 45, 62, 606, 1008, 1073, 1297
SsiI CCGC 4 cut(s) 5, 387, 1447, 1796
SspI AATATT 1 cut(s) 894
SspMI CTAG 3 cut(s) 221, 1002, 1064
SstI GAGCTC 1 cut(s) 1734
StyD4I CCNGG 2 cut(s) 200, 1414
StyI CCWWGG 1 cut(s) 1136
TaaI ACNGT 8 cut(s) 810, 857, 1108, 1279, 1442, 1504, 1773, 1817
TaiI ACGT 2 cut(s) 935, 1824
TaqI TCGA 5 cut(s) 135, 213, 324, 1244, 1751
TaqII GACCGA 1 cut(s) 1832
TasI AATT 6 cut(s) 45, 62, 606, 1008, 1073, 1297
TatI WGTACW 1 cut(s) 148
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 7 cut(s) 54, 185, 268, 576, 1601, 1870, 1883
Tru1I TTAA 4 cut(s) 599, 1011, 1256, 1850
Tru9I TTAA 4 cut(s) 599, 1011, 1256, 1850
TscAI CASTG 3 cut(s) 34, 349, 1364
TseFI GTSAC 7 cut(s) 32, 279, 338, 810, 1580, 1712, 1817
TseI GCWGC 5 cut(s) 463, 548, 822, 1130, 1449
Tsp45I GTSAC 7 cut(s) 32, 279, 338, 810, 1580, 1712, 1817
TspDTI ATGAA 5 cut(s) 46, 285, 576, 1262, 1562
TspGWI ACGGA 4 cut(s) 51, 1310, 1640, 1868
TspRI CASTG 3 cut(s) 34, 349, 1364
Van91I CCANNNNNTGG 1 cut(s) 1484
VpaK11BI GGWCC 2 cut(s) 381, 1232
XapI RAATTY 1 cut(s) 62
XceI RCATGY 1 cut(s) 1219
XcmI CCANNNNNNNNNTGG 3 cut(s) 800, 1178, 1289
XmiI GTMKAC 2 cut(s) 135, 886
XspI CTAG 3 cut(s) 221, 1002, 1064
Zsp2I ATGCAT 2 cut(s) 538, 1427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.