RH3DG190600

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0011045
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG190600.1

Sequence Viewer

Length: 1830 bp
ATGGCAGACCCTCACTATGTGCATCTTGACGATGTAGATCTACAGGAAGAGGATGAAGAAGCCTCAGAAGAACCATCCGTCATCGGCGACGTTGAAGACGAAGAAGAACCAGACGCACAAACGGATCCCAATTATGCTCTCTATCTTTCCAGAATGGGACCCACTCATGAGGCCGAAGAAGCCGACTCGGATTTTGAACGGCATCTGGTCAGAACCGCTTGCTTTCTGAGCGGCGGCGGCGGCGGTGACGGCAAGCGGAGAAGAATCGGAAGGCAGGAAACGGCGGCGGCGTTTGACACCGGAGAGAGCTCTCAGAGCAGTCAGTGGAATCCGAATGAAATTGATGGCCTGTTTTGCCCAATTTGCTTGGAGGCTTGGACTAATGACGGTGATCACTACATCTGCTGTCTTCCTTGTGGACACATTTTCGGTATGTCCTGCATCGAAAAATGGCTAGGCCAGAGTAAAAGACAGGGAAAGTGTCCTCAATGCAATGCCAAATGCAAGTTGAATGATGTCAGGAAACTTTTCACGTCACGAGTTATTTCTGTTGACGAAGAATCACAAAAGAGGATTCATTCACTTGAGGCTAAATGTGCTTCTTTTGAGAAGGAGAGGGAAGCAGAATTGCTAAAGGGACATCATGTACTAGAACTCAAAATTCAACTACTTATGGAGAGCAAAGCATCAGGATCAATCAATGCTACTGGGGCCTTTGAAAGAAGATTCGTATCTGGGCTAACATTTGGCTCTAATACCTGTGGTCAAGCATCTCCTTGCAGCTTCAAATTAGAGAAGGAGTTACCTGTAGCTGGTGCTCATTTGTTTGACGTTGATGTGTCTAACCAAATTTTGTTAATTTCGCGAAGGCATGCTGGGATAGGTGGAAAAGATGTGCTTACCAAAATGAGCCTGATACCTCCATATGACAGAGATGATATTCTTCTTCCTAGCTCTTTAAATTCTATCAGGGACCTACGTCTTTCTCCTTCTAACACTAACCTTGCACTCTTTGCTTGCCTAGGGAAGAAATTGGCAGTTCTCAGTATGGAGAACAACAACGTTATTGTTTCCTATGACCTACCGGCTGCTGCTTGGACATGCTCATGGGATCTCAACGATTCAAATTATATGTATGCTGGATTACAGAATGGCTATCTTCTGGTGTTTGACATGCGCCAAACCTCAGGGCCTGTTGAATCCCTGAAGGGGCTGACAAGAAACCCAATTCATACCGTGCATTCCCTTTCATATAATTCAGCTCTTCCCACTGGTGTTAGAACAGTGATATCTGCATCGTCAATTGGCTTATGTCTCTGGAATTTTGGTGGTGCTGCAAAGGGGCCAATTTTGGTCCCTGAAACAGAGAACCAAGGAGTATGTATATCTCTTGCATATTGCCCTAGAACAGATGACATTGTTGCTTCCTATCGCCCCAAACTCGAGTTTTCTGATGAGGCAGTTCTTACACAACCAACTCCTTCTCGTGCCATTGGACAAGGAACAGTCGGTTCCCATGTTCTTTTAAAGAGAGCAGGCAGCAGCAGTCATTTTCTAAAGTTGGGATCTTCATCCGGCAACGTGTGTGATATCGGACTGCCAAAATCTGCAATTATCAACATAGAAAATTGTGGCAGTTTGTTTGCATCTGAAGATAAACTATCTTCTGAACTAGTTTTAGAAGAGCTGTCGTCTTTTACTACTGTTCAGCGCCTTAACTTACAGCAAAGTCCTGTTCAGGATGTGAAGTACACATCTTTCTTAAGGAAAGGTCTCCTCAGTTGTTTGAATGGTAACACGCTGCAACTCTTCAGTACCAAGCTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

609

Amino Acids

66.49

Weight (kDa)

5.27

Isoelectric Point (pI)

54.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 119 - 165 1.4e-09 Ring finger domain
zf-RING_UBOX PF13445 119 - 162 4.3e-06 RING-type zinc-finger
WD40_RFWD3 PF23419 321 - 604 1.1e-31 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03450 AT5G03750
fragaria_vesca FvH4_6g16560 FvH4_6g16560
malus_domestica MD12G1109200.v1.1
prunus_persica Prupe.7G127800_v2.0.a1
pyrus_communis pycom12g10940
rosa_chinensis RchiOBHm_Chr3g0470481
rosa_laevigata RLG00000024263
rosa_multiflora Rmu_sc0002115.1_g000004 Rmu_sc0003381.1_g000011
rosa_roxburghii Rroxscaffold_4G00302270 Rroxscaffold_6G00410770
rosa_rugosa Rorug03G0109400 Rorug03G0109500
rosa_samantha Rh3AG159800 Rh3BG184300 Rh3CG174800 Rh3DG190600
rosa_wichuraiana Rw3G015120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 231
AccII CGCG 1 cut(s) 865
AciI CCGC 9 cut(s) 216, 231, 234, 237, 240, 243, 256, 284, 287
AclI AACGTT 1 cut(s) 1062
AclWI GGATC 5 cut(s) 119, 132, 700, 1119, 1573
AcsI RAATTY 4 cut(s) 660, 849, 961, 1321
AcuI CTGAAG 3 cut(s) 1226, 1671, 1795
AdeI CACNNNGTG 1 cut(s) 19
AfaI GTAC 3 cut(s) 648, 1751, 1816
AfiI CCNNNNNNNGG 2 cut(s) 812, 1209
AflII CTTAAG 1 cut(s) 1762
AflIII ACRYGT 1 cut(s) 1581
AgsI TTSAA 9 cut(s) 95, 197, 511, 665, 719, 787, 1125, 1199, 1789
AhlI ACTAGT 1 cut(s) 1672
AjiI CACGTC 1 cut(s) 534
AluBI AGCT 7 cut(s) 309, 783, 812, 954, 1262, 1687, 1822
AluI AGCT 7 cut(s) 309, 783, 812, 954, 1262, 1687, 1822
Alw21I GWGCWC 2 cut(s) 311, 820
Alw26I GTCTC 2 cut(s) 1319, 1778
AlwI GGATC 5 cut(s) 119, 132, 700, 1119, 1573
AlwNI CAGNNNCTG 1 cut(s) 1193
Ama87I CYCGRG 1 cut(s) 1442
AoxI GGCC 6 cut(s) 171, 346, 457, 711, 1190, 1343
ApeKI GCWGC 7 cut(s) 780, 1088, 1091, 1334, 1539, 1542, 1801
ApoI RAATTY 4 cut(s) 660, 849, 961, 1321
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
Asp700I GAANNNNTTC 1 cut(s) 527
AspA2I CCTAGG 1 cut(s) 1021
AspLEI GCGC 2 cut(s) 1179, 1713
AspS9I GGNCC 6 cut(s) 158, 711, 973, 1190, 1343, 1354
AsuHPI GGTGA 2 cut(s) 257, 401
AvaI CYCGRG 1 cut(s) 1442
AvaII GGWCC 3 cut(s) 158, 973, 1354
AvrII CCTAGG 1 cut(s) 1021
AxyI CCTNAGG 1 cut(s) 1186
BamHI GGATCC 1 cut(s) 124
BanII GRGCYC 1 cut(s) 311
BauI CACGAG 2 cut(s) 537, 1485
BbsI GAAGAC 2 cut(s) 102, 401
Bbv12I GWGCWC 2 cut(s) 311, 820
BbvI GCAGC 7 cut(s) 792, 1075, 1078, 1321, 1551, 1554, 1788
BccI CCATC 2 cut(s) 82, 338
BceAI ACGGC 3 cut(s) 215, 265, 297
BclI TGATCA 1 cut(s) 391
BcoDI GTCTC 2 cut(s) 1319, 1778
BcuI ACTAGT 1 cut(s) 1672
BfaI CTAG 6 cut(s) 455, 650, 951, 1022, 1404, 1673
BfmI CTRYAG 2 cut(s) 41, 807
BfoI RGCGCY 1 cut(s) 1714
BfrI CTTAAG 1 cut(s) 1762
BglII AGATCT 1 cut(s) 37
BlnI CCTAGG 1 cut(s) 1021
Bme18I GGWCC 3 cut(s) 158, 973, 1354
BmeT110I CYCGRG 1 cut(s) 1442
BmgBI CACGTC 1 cut(s) 534
BmgT120I GGNCC 6 cut(s) 158, 711, 973, 1190, 1343, 1354
BmiI GGNNCC 8 cut(s) 126, 159, 160, 712, 974, 1344, 1356, 1513
BmrI ACTGGG 1 cut(s) 717
BmsI GCATC 7 cut(s) 31, 211, 450, 695, 779, 1304, 1655
BmuI ACTGGG 1 cut(s) 717
BoxI GACNNNNGTC 1 cut(s) 978
BpiI GAAGAC 2 cut(s) 102, 401
BpuEI CTTGAG 1 cut(s) 605
BsaBI GATNNNNATC 3 cut(s) 36, 730, 1570
BsaI GGTCTC 1 cut(s) 1778
BsaJI CCNNGG 2 cut(s) 1021, 1372
BsaWI WCCGGW 1 cut(s) 299
Bsc4I CCNNNNNNNGG 2 cut(s) 812, 1209
Bse118I RCCGGY 1 cut(s) 1084
Bse1I ACTGG 2 cut(s) 712, 1276
Bse21I CCTNAGG 1 cut(s) 1186
Bse3DI GCAATG 1 cut(s) 499
Bse8I GATNNNNATC 3 cut(s) 36, 730, 1570
BseDI CCNNGG 2 cut(s) 1021, 1372
BseGI GGATG 4 cut(s) 58, 74, 1571, 1747
BseJI GATNNNNATC 3 cut(s) 36, 730, 1570
BseLI CCNNNNNNNGG 2 cut(s) 812, 1209
BseMI GCAATG 1 cut(s) 499
BseMII CTCAG 6 cut(s) 78, 218, 326, 1057, 1200, 1792
BseNI ACTGG 2 cut(s) 712, 1276
BseRI GAGGAG 1 cut(s) 1766
BseXI GCAGC 7 cut(s) 792, 1075, 1078, 1321, 1551, 1554, 1788
BseYI CCCAGC 1 cut(s) 875
Bsh1236I CGCG 1 cut(s) 865
BshFI GGCC 6 cut(s) 173, 348, 459, 713, 1192, 1345
BsiHKAI GWGCWC 2 cut(s) 311, 820
BsiHKCI CYCGRG 1 cut(s) 1442
BsiSI CCGG 3 cut(s) 300, 1085, 1575
BslFI GGGAC 4 cut(s) 171, 651, 986, 1340
BslI CCNNNNNNNGG 2 cut(s) 812, 1209
BsmAI GTCTC 2 cut(s) 1319, 1778
BsmFI GGGAC 4 cut(s) 171, 651, 986, 1340
BsmI GAATGC 1 cut(s) 1240
BsnI GGCC 6 cut(s) 173, 348, 459, 713, 1192, 1345
Bso31I GGTCTC 1 cut(s) 1778
BsoBI CYCGRG 1 cut(s) 1442
Bsp1286I GDGCHC 2 cut(s) 311, 820
Bsp143I GATC 6 cut(s) 37, 124, 391, 692, 1111, 1565
Bsp68I TCGCGA 1 cut(s) 865
BspACI CCGC 9 cut(s) 216, 231, 234, 237, 240, 243, 256, 284, 287
BspANI GGCC 6 cut(s) 173, 348, 459, 713, 1192, 1345
BspCNI CTCAG 6 cut(s) 77, 219, 325, 1056, 1199, 1791
BspFNI CGCG 1 cut(s) 865
BspHI TCATGA 2 cut(s) 166, 1826
BspLI GGNNCC 8 cut(s) 126, 159, 160, 712, 974, 1344, 1356, 1513
BspPI GGATC 5 cut(s) 119, 132, 700, 1119, 1573
BspQI GCTCTTC 2 cut(s) 1269, 1677
BspTI CTTAAG 1 cut(s) 1762
BspTNI GGTCTC 1 cut(s) 1778
BsrBI CCGCTC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 499
BsrFI RCCGGY 1 cut(s) 1084
BsrI ACTGG 2 cut(s) 712, 1276
BssAI RCCGGY 1 cut(s) 1084
BssECI CCNNGG 2 cut(s) 1021, 1372
BssMI GATC 6 cut(s) 37, 124, 391, 692, 1111, 1565
BssSI CACGAG 2 cut(s) 537, 1485
BssT1I CCWWGG 2 cut(s) 1021, 1372
Bst2BI CACGAG 2 cut(s) 537, 1485
Bst4CI ACNGT 5 cut(s) 389, 1237, 1285, 1507, 1705
Bst6I CTCTTC 4 cut(s) 42, 1269, 1677, 1814
BstAFI CTTAAG 1 cut(s) 1762
BstAPI GCANNNNNTGC 1 cut(s) 1013
BstC8I GCNNGC 5 cut(s) 220, 254, 873, 1018, 1537
BstDEI CTNAG 6 cut(s) 64, 227, 312, 1043, 1186, 1778
BstF5I GGATG 4 cut(s) 58, 74, 1571, 1747
BstFNI CGCG 1 cut(s) 865
BstH2I RGCGCY 1 cut(s) 1714
BstHHI GCGC 2 cut(s) 1179, 1713
BstKTI GATC 6 cut(s) 40, 127, 394, 695, 1114, 1568
BstMAI GTCTC 2 cut(s) 1319, 1778
BstMBI GATC 6 cut(s) 37, 124, 391, 692, 1111, 1565
BstNSI RCATGY 3 cut(s) 875, 1104, 1177
BstPAI GACNNNNGTC 1 cut(s) 978
BstSFI CTRYAG 2 cut(s) 41, 807
BstUI CGCG 1 cut(s) 865
BstV1I GCAGC 7 cut(s) 792, 1075, 1078, 1321, 1551, 1554, 1788
BstV2I GAAGAC 2 cut(s) 102, 401
BstX2I RGATCY 4 cut(s) 37, 124, 1111, 1565
BstYI RGATCY 4 cut(s) 37, 124, 1111, 1565
Bsu36I CCTNAGG 1 cut(s) 1186
BsuRI GGCC 6 cut(s) 173, 348, 459, 713, 1192, 1345
BtrI CACGTC 1 cut(s) 534
BtsCI GGATG 4 cut(s) 58, 74, 1571, 1747
BtsIMutI CAGTG 3 cut(s) 329, 1269, 1290
BtuMI TCGCGA 1 cut(s) 865
Cac8I GCNNGC 5 cut(s) 220, 254, 873, 1018, 1537
CaiI CAGNNNCTG 1 cut(s) 1193
CciI TCATGA 2 cut(s) 166, 1826
CfoI GCGC 2 cut(s) 1179, 1713
Cfr10I RCCGGY 1 cut(s) 1084
Cfr13I GGNCC 6 cut(s) 158, 711, 973, 1190, 1343, 1354
CseI GACGC 1 cut(s) 122
Csp6I GTAC 3 cut(s) 647, 1750, 1815
CviAII CATG 8 cut(s) 167, 644, 872, 1101, 1107, 1174, 1517, 1827
CviQI GTAC 3 cut(s) 647, 1750, 1815
DdeI CTNAG 6 cut(s) 64, 227, 312, 1043, 1186, 1778
DpnI GATC 6 cut(s) 39, 126, 393, 694, 1113, 1567
DpnII GATC 6 cut(s) 37, 124, 391, 692, 1111, 1565
DraI TTTAAA 2 cut(s) 960, 1527
DraIII CACNNNGTG 1 cut(s) 19
Eam1104I CTCTTC 4 cut(s) 42, 1269, 1677, 1814
EarI CTCTTC 4 cut(s) 42, 1269, 1677, 1814
Ecl136II GAGCTC 1 cut(s) 309
Eco130I CCWWGG 2 cut(s) 1021, 1372
Eco24I GRGCYC 1 cut(s) 311
Eco31I GGTCTC 1 cut(s) 1778
Eco32I GATATC 2 cut(s) 1290, 1591
Eco47I GGWCC 3 cut(s) 158, 973, 1354
Eco53kI GAGCTC 1 cut(s) 309
Eco57I CTGAAG 3 cut(s) 1226, 1671, 1795
Eco81I CCTNAGG 1 cut(s) 1186
Eco88I CYCGRG 1 cut(s) 1442
EcoICRI GAGCTC 1 cut(s) 309
EcoO109I RGGNCCY 4 cut(s) 158, 711, 973, 1190
EcoRV GATATC 2 cut(s) 1290, 1591
EcoT14I CCWWGG 2 cut(s) 1021, 1372
EcoT38I GRGCYC 1 cut(s) 311
ErhI CCWWGG 2 cut(s) 1021, 1372
FaeI CATG 8 cut(s) 170, 647, 875, 1104, 1110, 1177, 1520, 1830
FalI AAGNNNNNCTT 2 cut(s) 882, 914
FaqI GGGAC 4 cut(s) 171, 651, 986, 1340
FatI CATG 8 cut(s) 166, 643, 871, 1100, 1106, 1173, 1516, 1826
FauNDI CATATG 1 cut(s) 925
FbaI TGATCA 1 cut(s) 391
FokI GGATG 4 cut(s) 61, 65, 1558, 1754
FriOI GRGCYC 1 cut(s) 311
FspBI CTAG 6 cut(s) 455, 650, 951, 1022, 1404, 1673
GlaI GCGC 2 cut(s) 1178, 1712
GsaI CCCAGC 1 cut(s) 879
HaeII RGCGCY 1 cut(s) 1714
HaeIII GGCC 6 cut(s) 173, 348, 459, 713, 1192, 1345
HapII CCGG 3 cut(s) 300, 1085, 1575
HgaI GACGC 1 cut(s) 122
HhaI GCGC 2 cut(s) 1179, 1713
Hin1II CATG 8 cut(s) 170, 647, 875, 1104, 1110, 1177, 1520, 1830
Hin6I GCGC 2 cut(s) 1177, 1711
HinP1I GCGC 2 cut(s) 1177, 1711
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HindIII AAGCTT 1 cut(s) 1820
HinfI GANTC 8 cut(s) 185, 264, 328, 560, 574, 726, 1121, 1199
HpaII CCGG 3 cut(s) 300, 1085, 1575
HphI GGTGA 2 cut(s) 257, 401
Hpy166II GTNNAC 3 cut(s) 419, 553, 1752
Hpy8I GTNNAC 3 cut(s) 419, 553, 1752
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 7 cut(s) 264, 604, 790, 861, 999, 1201, 1491
HpyCH4III ACNGT 5 cut(s) 389, 1237, 1285, 1507, 1705
HpyCH4IV ACGT 6 cut(s) 90, 533, 831, 979, 1062, 1581
HpyF3I CTNAG 6 cut(s) 64, 227, 312, 1043, 1186, 1778
HpySE526I ACGT 6 cut(s) 90, 533, 831, 979, 1062, 1581
Hsp92II CATG 8 cut(s) 170, 647, 875, 1104, 1110, 1177, 1520, 1830
HspAI GCGC 2 cut(s) 1177, 1711
KflI GGGWCCC 1 cut(s) 158
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 6 cut(s) 37, 124, 391, 692, 1111, 1565
LguI GCTCTTC 2 cut(s) 1269, 1677
Lsp1109I GCAGC 7 cut(s) 792, 1075, 1078, 1321, 1551, 1554, 1788
LweI GCATC 7 cut(s) 31, 211, 450, 695, 779, 1304, 1655
MaeI CTAG 6 cut(s) 455, 650, 951, 1022, 1404, 1673
MaeII ACGT 6 cut(s) 90, 533, 831, 979, 1062, 1581
MaeIII GTNAC 4 cut(s) 245, 534, 801, 1793
MalI GATC 6 cut(s) 39, 126, 393, 694, 1113, 1567
MbiI CCGCTC 1 cut(s) 231
MboI GATC 6 cut(s) 37, 124, 391, 692, 1111, 1565
MfeI CAATTG 1 cut(s) 1302
MflI RGATCY 4 cut(s) 37, 124, 1111, 1565
MhlI GDGCHC 2 cut(s) 311, 820
MlyI GAGTC 1 cut(s) 179
MroXI GAANNNNTTC 1 cut(s) 527
MseI TTAA 5 cut(s) 857, 959, 1526, 1716, 1763
MslI CAYNNNNRTG 1 cut(s) 1105
MspCI CTTAAG 1 cut(s) 1762
MspI CCGG 3 cut(s) 300, 1085, 1575
MunI CAATTG 1 cut(s) 1302
Mva1269I GAATGC 1 cut(s) 1240
MvnI CGCG 1 cut(s) 865
NdeI CATATG 1 cut(s) 925
NdeII GATC 6 cut(s) 37, 124, 391, 692, 1111, 1565
NlaIII CATG 8 cut(s) 170, 647, 875, 1104, 1110, 1177, 1520, 1830
NlaIV GGNNCC 8 cut(s) 126, 159, 160, 712, 974, 1344, 1356, 1513
NmuCI GTSAC 2 cut(s) 245, 534
NruI TCGCGA 1 cut(s) 865
NspI RCATGY 3 cut(s) 875, 1104, 1177
PaeI GCATGC 1 cut(s) 875
PaeR7I CTCGAG 1 cut(s) 1442
PagI TCATGA 2 cut(s) 166, 1826
PciSI GCTCTTC 2 cut(s) 1269, 1677
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 287
PctI GAATGC 1 cut(s) 1240
PdmI GAANNNNTTC 1 cut(s) 527
PfeI GAWTC 7 cut(s) 264, 328, 560, 574, 726, 1121, 1199
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
PpuMI RGGWCCY 2 cut(s) 158, 973
PshAI GACNNNNGTC 1 cut(s) 978
Psp124BI GAGCTC 1 cut(s) 311
Psp1406I AACGTT 1 cut(s) 1062
Psp5II RGGWCCY 2 cut(s) 158, 973
PspFI CCCAGC 1 cut(s) 875
PspN4I GGNNCC 8 cut(s) 126, 159, 160, 712, 974, 1344, 1356, 1513
PspPI GGNCC 6 cut(s) 158, 711, 973, 1190, 1343, 1354
PspPPI RGGWCCY 2 cut(s) 158, 973
PspXI VCTCGAGB 1 cut(s) 1442
PstNI CAGNNNCTG 1 cut(s) 1193
PsuI RGATCY 4 cut(s) 37, 124, 1111, 1565
RruI TCGCGA 1 cut(s) 865
RsaI GTAC 3 cut(s) 648, 1751, 1816
RsaNI GTAC 3 cut(s) 647, 1750, 1815
RseI CAYNNNNRTG 1 cut(s) 1105
SacI GAGCTC 1 cut(s) 311
SapI GCTCTTC 2 cut(s) 1269, 1677
SaqAI TTAA 5 cut(s) 857, 959, 1526, 1716, 1763
Sau3AI GATC 6 cut(s) 37, 124, 391, 692, 1111, 1565
Sau96I GGNCC 6 cut(s) 158, 711, 973, 1190, 1343, 1354
SchI GAGTC 1 cut(s) 179
SduI GDGCHC 2 cut(s) 311, 820
SfaNI GCATC 7 cut(s) 31, 211, 450, 695, 779, 1304, 1655
SfcI CTRYAG 2 cut(s) 41, 807
Sfr274I CTCGAG 1 cut(s) 1442
SinI GGWCC 3 cut(s) 158, 973, 1354
SlaI CTCGAG 1 cut(s) 1442
SmiMI CAYNNNNRTG 1 cut(s) 1105
SmlI CTYRAG 3 cut(s) 584, 1442, 1762
SmoI CTYRAG 3 cut(s) 584, 1442, 1762
SpeI ACTAGT 1 cut(s) 1672
SphI GCATGC 1 cut(s) 875
SsiI CCGC 9 cut(s) 216, 231, 234, 237, 240, 243, 256, 284, 287
SspMI CTAG 6 cut(s) 455, 650, 951, 1022, 1404, 1673
SstI GAGCTC 1 cut(s) 311
StyI CCWWGG 2 cut(s) 1021, 1372
TaaI ACNGT 5 cut(s) 389, 1237, 1285, 1507, 1705
TaiI ACGT 6 cut(s) 93, 536, 834, 982, 1065, 1584
TaqI TCGA 2 cut(s) 444, 1443
TatI WGTACW 2 cut(s) 646, 1749
TauI GCSGC 6 cut(s) 234, 237, 240, 243, 287, 290
TfiI GAWTC 7 cut(s) 264, 328, 560, 574, 726, 1121, 1199
Tru1I TTAA 5 cut(s) 857, 959, 1526, 1716, 1763
Tru9I TTAA 5 cut(s) 857, 959, 1526, 1716, 1763
TscAI CASTG 3 cut(s) 329, 1276, 1290
TseFI GTSAC 2 cut(s) 245, 534
TseI GCWGC 7 cut(s) 780, 1088, 1091, 1334, 1539, 1542, 1801
Tsp45I GTSAC 2 cut(s) 245, 534
TspDTI ATGAA 7 cut(s) 69, 351, 566, 1220, 1239, 1560, 1815
TspGWI ACGGA 2 cut(s) 67, 137
TspRI CASTG 3 cut(s) 329, 1276, 1290
Vha464I CTTAAG 1 cut(s) 1762
VpaK11BI GGWCC 3 cut(s) 158, 973, 1354
XapI RAATTY 4 cut(s) 660, 849, 961, 1321
XceI RCATGY 3 cut(s) 875, 1104, 1177
XhoI CTCGAG 1 cut(s) 1442
XmaJI CCTAGG 1 cut(s) 1021
XmnI GAANNNNTTC 1 cut(s) 527
XspI CTAG 6 cut(s) 455, 650, 951, 1022, 1404, 1673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.