AT5G03750

MDM2/MDM4 family protein binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011045
Physical Location & Seq
Forward (+)
984796 .. 985497
702 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03750.1

Sequence Viewer

Length: 510 bp
ATGGAAGTATTCGATGAACCTGATGAGGAAGAAGAACGCCAAATTAGCCAATACATCGATGTGGTAGACTCACCTAGGGTTTCACAGAACGAGAGTGAGTATCAAAGAAGCTGCACCTTGTCTTGTTCTTGGGATCTCAACAACTATCACAATGTTTATGCTGGACTACAGAATGGAATGGTTTTAGTATTTGACATGCGTCAAAACAGAGGACCTTTGGTTTCGTTGGCTGACGTAACAAGCAATTCGGTTCATATTGTAGATGGATTTCATGTTTGTCTCAAGAGAAGAGGTGATGGTTCTTATTCTCAGAAACAGTCATCCACACAAGCTATATCCACCCGTGAACTCATCTTGCAAGATCCTTGGTCTTTTGCTGTCTCACAACGGTTCGCATTGTCAAGTCAACTTCCGCTTCAAGATGTGAAATACGCTAACCTCAATGGCTCGGGTCTGCTCGGTCTCTTAACCAATGATAGATTCCAGTTTCTTAGAAGCGAATCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

169

Amino Acids

19.16

Weight (kDa)

4.77

Isoelectric Point (pI)

68.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RFWD3 PF23419 40 - 82 6.2e-09 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03450 AT5G03750
fragaria_vesca FvH4_6g16560 FvH4_6g16560
malus_domestica MD12G1109200.v1.1
prunus_persica Prupe.7G127800_v2.0.a1
pyrus_communis pycom12g10940
rosa_chinensis RchiOBHm_Chr3g0470481
rosa_laevigata RLG00000024263
rosa_multiflora Rmu_sc0002115.1_g000004 Rmu_sc0003381.1_g000011
rosa_roxburghii Rroxscaffold_4G00302270 Rroxscaffold_6G00410770
rosa_rugosa Rorug03G0109400 Rorug03G0109500
rosa_samantha Rh3AG159800 Rh3BG184300 Rh3CG174800 Rh3DG190600
rosa_wichuraiana Rw3G015120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 66
AciI CCGC 1 cut(s) 413
AclWI GGATC 2 cut(s) 141, 356
AgsI TTSAA 1 cut(s) 419
AjuI GAANNNNNNNTTGG 2 cut(s) 464, 496
AluBI AGCT 2 cut(s) 111, 332
AluI AGCT 2 cut(s) 111, 332
Alw26I GTCTC 3 cut(s) 284, 385, 467
AlwI GGATC 2 cut(s) 141, 356
Ama87I CYCGRG 1 cut(s) 448
ApeKI GCWGC 1 cut(s) 111
AspA2I CCTAGG 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 63, 305
AvaI CYCGRG 1 cut(s) 448
AvaII GGWCC 1 cut(s) 212
AvrII CCTAGG 1 cut(s) 74
BbvI GCAGC 1 cut(s) 98
BccI CCATC 2 cut(s) 257, 290
BcoDI GTCTC 3 cut(s) 284, 385, 467
BfaI CTAG 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 167
BisI GCNGC 1 cut(s) 112
BlnI CCTAGG 1 cut(s) 74
BlsI GCNGC 1 cut(s) 113
Bme18I GGWCC 1 cut(s) 212
BmeT110I CYCGRG 1 cut(s) 448
BmgT120I GGNCC 1 cut(s) 212
BoxI GACNNNNGTC 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 266
Bsa29I ATCGAT 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 467
BsaJI CCNNGG 2 cut(s) 74, 365
Bse1I ACTGG 1 cut(s) 484
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 2 cut(s) 74, 365
BseGI GGATG 1 cut(s) 320
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 1 cut(s) 484
BseXI GCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 97
BshVI ATCGAT 1 cut(s) 57
BsiHKCI CYCGRG 1 cut(s) 448
BsmAI GTCTC 3 cut(s) 284, 385, 467
Bso31I GGTCTC 1 cut(s) 467
BsoBI CYCGRG 1 cut(s) 448
Bsp143I GATC 2 cut(s) 133, 361
BspACI CCGC 1 cut(s) 413
BspCNI CTCAG 1 cut(s) 322
BspDI ATCGAT 1 cut(s) 57
BspPI GGATC 2 cut(s) 141, 356
BspTNI GGTCTC 1 cut(s) 467
BsrI ACTGG 1 cut(s) 484
BssECI CCNNGG 2 cut(s) 74, 365
BssMI GATC 2 cut(s) 133, 361
BssT1I CCWWGG 2 cut(s) 74, 365
Bst4CI ACNGT 2 cut(s) 318, 390
Bst6I CTCTTC 1 cut(s) 283
BstDEI CTNAG 2 cut(s) 309, 491
BstF5I GGATG 1 cut(s) 320
BstKTI GATC 2 cut(s) 136, 364
BstMAI GTCTC 3 cut(s) 284, 385, 467
BstMBI GATC 2 cut(s) 133, 361
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstNSI RCATGY 1 cut(s) 199
BstPAI GACNNNNGTC 1 cut(s) 198
BstSFI CTRYAG 1 cut(s) 167
BstV1I GCAGC 1 cut(s) 98
BstX2I RGATCY 2 cut(s) 133, 361
BstYI RGATCY 2 cut(s) 133, 361
Bsu15I ATCGAT 1 cut(s) 57
BsuTUI ATCGAT 1 cut(s) 57
BtsCI GGATG 1 cut(s) 320
Cfr13I GGNCC 1 cut(s) 212
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 1 cut(s) 188
CviAII CATG 3 cut(s) 196, 272, 507
CviJI RGCY 5 cut(s) 48, 111, 230, 332, 447
CviKI_1 RGCY 5 cut(s) 48, 111, 230, 332, 447
DdeI CTNAG 2 cut(s) 309, 491
DpnI GATC 2 cut(s) 135, 363
DpnII GATC 2 cut(s) 133, 361
Eam1104I CTCTTC 1 cut(s) 283
EarI CTCTTC 1 cut(s) 283
Eco130I CCWWGG 2 cut(s) 74, 365
Eco31I GGTCTC 1 cut(s) 467
Eco47I GGWCC 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 448
EcoO109I RGGNCCY 1 cut(s) 212
EcoT14I CCWWGG 2 cut(s) 74, 365
ErhI CCWWGG 2 cut(s) 74, 365
FaeI CATG 3 cut(s) 199, 275, 510
FaiI YATR 6 cut(s) 159, 197, 255, 273, 335, 508
FatI CATG 3 cut(s) 195, 271, 506
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 1 cut(s) 112
FokI GGATG 1 cut(s) 307
Fsp4HI GCNGC 1 cut(s) 112
FspBI CTAG 1 cut(s) 75
GluI GCNGC 1 cut(s) 112
HgaI GACGC 1 cut(s) 188
Hin1II CATG 3 cut(s) 199, 275, 510
HincII GTYRAC 1 cut(s) 407
HindII GTYRAC 1 cut(s) 407
HinfI GANTC 3 cut(s) 68, 480, 500
HphI GGTGA 2 cut(s) 63, 305
Hpy166II GTNNAC 3 cut(s) 67, 347, 407
Hpy188I TCNGA 1 cut(s) 312
Hpy188III TCNNGA 2 cut(s) 283, 419
Hpy8I GTNNAC 3 cut(s) 67, 347, 407
HpyCH4III ACNGT 2 cut(s) 318, 390
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 2 cut(s) 114, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 2 cut(s) 309, 491
HpySE526I ACGT 1 cut(s) 234
Hsp92II CATG 3 cut(s) 199, 275, 510
Kzo9I GATC 2 cut(s) 133, 361
LpnPI CCDG 3 cut(s) 33, 147, 497
Lsp1109I GCAGC 1 cut(s) 98
MaeI CTAG 1 cut(s) 75
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 235
MalI GATC 2 cut(s) 135, 363
MboI GATC 2 cut(s) 133, 361
MboII GAAGA 3 cut(s) 41, 44, 300
MflI RGATCY 2 cut(s) 133, 361
MluCI AATT 2 cut(s) 42, 244
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 4 cut(s) 19, 203, 284, 449
MseI TTAA 1 cut(s) 467
MslI CAYNNNNRTG 1 cut(s) 59
MwoI GCNNNNNNNGC 1 cut(s) 45
NdeII GATC 2 cut(s) 133, 361
NlaIII CATG 3 cut(s) 199, 275, 510
NspI RCATGY 1 cut(s) 199
PfeI GAWTC 2 cut(s) 480, 500
PkrI GCNGC 1 cut(s) 113
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PpuMI RGGWCCY 1 cut(s) 212
PshAI GACNNNNGTC 1 cut(s) 198
Psp5II RGGWCCY 1 cut(s) 212
PspPI GGNCC 1 cut(s) 212
PspPPI RGGWCCY 1 cut(s) 212
PsuI RGATCY 2 cut(s) 133, 361
RseI CAYNNNNRTG 1 cut(s) 59
SaqAI TTAA 1 cut(s) 467
SatI GCNGC 1 cut(s) 112
Sau3AI GATC 2 cut(s) 133, 361
Sau96I GGNCC 1 cut(s) 212
SchI GAGTC 1 cut(s) 62
SetI ASST 9 cut(s) 22, 76, 113, 119, 217, 237, 295, 334, 441
SfcI CTRYAG 1 cut(s) 167
SinI GGWCC 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 2 cut(s) 42, 244
SsiI CCGC 1 cut(s) 413
SspMI CTAG 1 cut(s) 75
StyI CCWWGG 2 cut(s) 74, 365
TaaI ACNGT 2 cut(s) 318, 390
TaiI ACGT 1 cut(s) 237
TaqI TCGA 2 cut(s) 12, 57
TaqII GACCGA 1 cut(s) 449
TasI AATT 2 cut(s) 42, 244
TfiI GAWTC 2 cut(s) 480, 500
Tru1I TTAA 1 cut(s) 467
Tru9I TTAA 1 cut(s) 467
TseI GCWGC 1 cut(s) 111
TspDTI ATGAA 3 cut(s) 30, 242, 260
VpaK11BI GGWCC 1 cut(s) 212
XceI RCATGY 1 cut(s) 199
XmaJI CCTAGG 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 66
XspI CTAG 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.