Rw3G015120

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0011045
Physical Location & Seq
Reverse (-)
15520397 .. 15525476
5080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G015120.1

Sequence Viewer

Length: 1938 bp
ATGGCAGACCCTCACTATGTGCATCTTGACGATGTAGATCTACAGGAAGAGGATGAAGAAGCCTCGGAAGAACCATCCGTCATCGGCGACGTTGAAGACTCAGAACAAGAAGACGAAGACGAACCAGACGCCCAAACGGATCCCAATTATGCTCTCTATCTTTCCAGAATGGGACCCACTCATGAGGCCGAAGAAGCCGACTCGGATTTTGAACGGCATCTGGTCAGAACCGCTTGCTTTCTGAGCGGCGGCGGCGGCGGCGGTGACGGCAAGCGGAGGAGAATCGGAAGGCAGGAAACGGCGGCGTTTGACACCGGAGAGAGCTCTCAGAGCAGTCAGTGGAATCCGAGTGAAATTGATGGCCTGTTTTGCCCAATTTGCTTGGAGGCTTGGACTAATGACGGTGATCACTACATCTGCTGTCTTCCTTGTGGACACATTTTCGGTATGTCCTGCATCGAAAAATGGCTAGGCCAGAGTAAAAGACAGGGAAAGTGTCCTCAATGCAATGCCAAATGCAAGTTGAATGATGTCAGGAAACTTTTCACGTCACGAGTTATTTCTGTTGACGAAGAATCACAAAAGAGGATTCGTTCACTTGAGGCTAAATGTGCTTCTTTTGAGAAGGAGCGAGCAGAATGGCATAAGAGGGAGGCAGTATTGCTAAAGGGACAGCATGTACTAGAACTCAAAATTCGACTACTTATGGAGTCAACATCTTTCTCACATTTGCTCACTTTGTTTGGAGATAATCAGAGCAAAGCATCAGGATCAATCAATGCTACTGGGACCTTTGAAAGAAGATTCGTATCTGGTAATAACATCTTAGAGGGAAGGTCTTATGGGCTAACATTTGGCTCTAATACCTGTGGTCATGCATCTCCTTGCAGCTTCAAATTAGAGACGGAGTTACCTGTAGCTGGTGCTCATTTGTTTGACGTTGATGCGTCTAACCAAATTTTGTTAATTTCGCGAAGGCATGCTGGGATAGGTGGAAAAGATGTGCTTACCAAAATGAGCCTGATACCTCCATATGACAGAGATGATATTCTTCTTCATAGCTCTTTAAATTCTATCAGGGACCTACGTCTTTCTCCTTCTAACACTAACCTTGCACTCTTTGCTTGCCTTGGGAAGAAAATGGCAGTTCTCAGTATGGAGAACAACAACGTCATTGTTTCCTATGACCTACCGGCTGCTGCTTGGACATGCTCATGGGATCTCAACGATTCAAATTATATGTATGCTGGATTACAGAATGGCTATCTTCTGGTGTTTGACATGCGCCAAACCTCAGGGCCTGTTGAATCCCTGAAGGGGCTGACAAGAAACCCAGTTCATACCGTGCATTCCCTTTCATATAATTCATCTCTTCCCACTGGTGTTAGAACAGTGATATCCGCATCGTCAATTGGCTTATGTCTCTGGAATTTTGGTGGTGCTGCAAAGGGGCCAATTTTGGTCCCTGAAACAGAGAACCAAGGAGTTTGTATATCTCTTGCATATTGCCCTAGAACAGATGACATTGTTGCTTCCTATCGCCCCAAAGTCGAGTTTTCTGATGAGGCAGTTCTTACACAACCAACTCCTTCACGTGCCATCGGACAAGGAACAGTCGGTTCCCATGTTCTTTTAAAGAGAGCAGGCAGCAGCAGTCATTTTCTAAAGTTGGGATCTTCATCCGGCAACGTGTGTGATATCGGACTGCCAAAATCTGCAATTATCAACATAGAAAATTGTGGCAGTTTGTTTGCATCTGAAGATAAACTATCTTCTGAACTAGTTTTAGAAGAGCTGTCGTCTTTTACTACTGTTCAGCGCCTTAACTTACAGCGAAGTCCTGTTCAGGATGTGAAGTACACATCTTTCTTTAGGAAAGGTCTCCTCAGTTGTTTGAATGGAAACACGCTGCAACTCTACAGTACCAAGCTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

645

Amino Acids

70.7

Weight (kDa)

5.37

Isoelectric Point (pI)

57.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 124 - 170 1.5e-09 Ring finger domain
zf-RING_UBOX PF13445 124 - 167 4.6e-06 RING-type zinc-finger
WD40_RFWD3 PF23419 337 - 640 1.4e-33 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03450 AT5G03750
fragaria_vesca FvH4_6g16560 FvH4_6g16560
malus_domestica MD12G1109200.v1.1
prunus_persica Prupe.7G127800_v2.0.a1
pyrus_communis pycom12g10940
rosa_chinensis RchiOBHm_Chr3g0470481
rosa_laevigata RLG00000024263
rosa_multiflora Rmu_sc0002115.1_g000004 Rmu_sc0003381.1_g000011
rosa_roxburghii Rroxscaffold_4G00302270 Rroxscaffold_6G00410770
rosa_rugosa Rorug03G0109400 Rorug03G0109500
rosa_samantha Rh3AG159800 Rh3BG184300 Rh3CG174800 Rh3DG190600
rosa_wichuraiana Rw3G015120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 246
AccII CGCG 1 cut(s) 973
AclWI GGATC 5 cut(s) 134, 147, 778, 1227, 1681
AcsI RAATTY 4 cut(s) 693, 957, 1069, 1429
AcuI CTGAAG 2 cut(s) 1334, 1779
AcvI CACGTG 1 cut(s) 1595
AcyI GRCGYC 1 cut(s) 129
AdeI CACNNNGTG 1 cut(s) 19
AfaI GTAC 3 cut(s) 681, 1859, 1924
AfiI CCNNNNNNNGG 2 cut(s) 920, 1317
AflIII ACRYGT 1 cut(s) 1689
AgsI TTSAA 8 cut(s) 95, 212, 526, 797, 895, 1233, 1307, 1897
AhlI ACTAGT 1 cut(s) 1780
AjiI CACGTC 1 cut(s) 549
AluBI AGCT 6 cut(s) 324, 891, 920, 1062, 1795, 1930
AluI AGCT 6 cut(s) 324, 891, 920, 1062, 1795, 1930
Alw21I GWGCWC 2 cut(s) 326, 928
Alw26I GTCTC 3 cut(s) 896, 1427, 1886
AlwI GGATC 5 cut(s) 134, 147, 778, 1227, 1681
AlwNI CAGNNNCTG 1 cut(s) 1301
AoxI GGCC 5 cut(s) 186, 361, 472, 1298, 1451
ApeKI GCWGC 7 cut(s) 888, 1196, 1199, 1442, 1647, 1650, 1909
ApoI RAATTY 4 cut(s) 693, 957, 1069, 1429
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 1 cut(s) 542
AspLEI GCGC 2 cut(s) 1287, 1821
AspS9I GGNCC 6 cut(s) 173, 789, 1081, 1298, 1451, 1462
AsuHPI GGTGA 2 cut(s) 275, 416
AvaII GGWCC 4 cut(s) 173, 789, 1081, 1462
AxyI CCTNAGG 1 cut(s) 1294
BamHI GGATCC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 326
BauI CACGAG 1 cut(s) 552
BbrPI CACGTG 1 cut(s) 1595
BbsI GAAGAC 4 cut(s) 102, 117, 123, 416
Bbv12I GWGCWC 2 cut(s) 326, 928
BbvI GCAGC 7 cut(s) 900, 1183, 1186, 1429, 1659, 1662, 1896
BccI CCATC 3 cut(s) 82, 353, 1607
BceAI ACGGC 3 cut(s) 230, 283, 315
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 3 cut(s) 896, 1427, 1886
BcuI ACTAGT 1 cut(s) 1780
BfaI CTAG 4 cut(s) 470, 683, 1512, 1781
BfmI CTRYAG 3 cut(s) 41, 915, 1918
BfoI RGCGCY 1 cut(s) 1822
BglII AGATCT 1 cut(s) 37
Bme18I GGWCC 4 cut(s) 173, 789, 1081, 1462
BmgBI CACGTC 1 cut(s) 549
BmgT120I GGNCC 6 cut(s) 173, 789, 1081, 1298, 1451, 1462
BmiI GGNNCC 8 cut(s) 141, 174, 175, 790, 1082, 1452, 1464, 1621
BmrI ACTGGG 2 cut(s) 795, 1328
BmsI GCATC 8 cut(s) 31, 226, 465, 773, 887, 934, 1412, 1763
BmuI ACTGGG 2 cut(s) 795, 1328
BoxI GACNNNNGTC 1 cut(s) 1086
BpiI GAAGAC 4 cut(s) 102, 117, 123, 416
BpuEI CTTGAG 1 cut(s) 620
BsaAI YACGTR 1 cut(s) 1595
BsaBI GATNNNNATC 3 cut(s) 36, 808, 1678
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 1886
BsaJI CCNNGG 3 cut(s) 63, 1129, 1480
BsaWI WCCGGW 1 cut(s) 314
Bsc4I CCNNNNNNNGG 2 cut(s) 920, 1317
Bse118I RCCGGY 1 cut(s) 1192
Bse1I ACTGG 3 cut(s) 790, 1334, 1384
Bse21I CCTNAGG 1 cut(s) 1294
Bse3DI GCAATG 1 cut(s) 514
Bse8I GATNNNNATC 3 cut(s) 36, 808, 1678
BseDI CCNNGG 3 cut(s) 63, 1129, 1480
BseGI GGATG 4 cut(s) 58, 74, 1679, 1855
BseJI GATNNNNATC 3 cut(s) 36, 808, 1678
BseLI CCNNNNNNNGG 2 cut(s) 920, 1317
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 6 cut(s) 114, 233, 341, 1165, 1308, 1900
BseNI ACTGG 3 cut(s) 790, 1334, 1384
BseRI GAGGAG 2 cut(s) 292, 1874
BseXI GCAGC 7 cut(s) 900, 1183, 1186, 1429, 1659, 1662, 1896
BseYI CCCAGC 1 cut(s) 983
Bsh1236I CGCG 1 cut(s) 973
BshFI GGCC 5 cut(s) 188, 363, 474, 1300, 1453
BsiHKAI GWGCWC 2 cut(s) 326, 928
BsiSI CCGG 3 cut(s) 315, 1193, 1683
BslFI GGGAC 5 cut(s) 186, 684, 802, 1094, 1448
BslI CCNNNNNNNGG 2 cut(s) 920, 1317
BsmAI GTCTC 3 cut(s) 896, 1427, 1886
BsmBI CGTCTC 1 cut(s) 896
BsmFI GGGAC 5 cut(s) 186, 684, 802, 1094, 1448
BsmI GAATGC 1 cut(s) 1348
BsnI GGCC 5 cut(s) 188, 363, 474, 1300, 1453
Bso31I GGTCTC 1 cut(s) 1886
Bsp1286I GDGCHC 2 cut(s) 326, 928
Bsp143I GATC 6 cut(s) 37, 139, 406, 770, 1219, 1673
Bsp68I TCGCGA 1 cut(s) 973
BspANI GGCC 5 cut(s) 188, 363, 474, 1300, 1453
BspCNI CTCAG 6 cut(s) 113, 234, 340, 1164, 1307, 1899
BspFNI CGCG 1 cut(s) 973
BspHI TCATGA 2 cut(s) 181, 1934
BspLI GGNNCC 8 cut(s) 141, 174, 175, 790, 1082, 1452, 1464, 1621
BspPI GGATC 5 cut(s) 134, 147, 778, 1227, 1681
BspQI GCTCTTC 1 cut(s) 1785
BspTNI GGTCTC 1 cut(s) 1886
BsrBI CCGCTC 1 cut(s) 246
BsrDI GCAATG 1 cut(s) 514
BsrFI RCCGGY 1 cut(s) 1192
BsrI ACTGG 3 cut(s) 790, 1334, 1384
BssAI RCCGGY 1 cut(s) 1192
BssECI CCNNGG 3 cut(s) 63, 1129, 1480
BssMI GATC 6 cut(s) 37, 139, 406, 770, 1219, 1673
BssNI GRCGYC 1 cut(s) 129
BssSI CACGAG 1 cut(s) 552
BssT1I CCWWGG 2 cut(s) 1129, 1480
Bst2BI CACGAG 1 cut(s) 552
Bst4CI ACNGT 6 cut(s) 404, 1345, 1393, 1615, 1813, 1922
Bst6I CTCTTC 3 cut(s) 42, 1377, 1785
BstACI GRCGYC 1 cut(s) 129
BstAPI GCANNNNNTGC 1 cut(s) 1121
BstBAI YACGTR 1 cut(s) 1595
BstC8I GCNNGC 6 cut(s) 235, 272, 633, 981, 1126, 1645
BstDEI CTNAG 7 cut(s) 100, 242, 327, 826, 1151, 1294, 1886
BstF5I GGATG 4 cut(s) 58, 74, 1679, 1855
BstFNI CGCG 1 cut(s) 973
BstH2I RGCGCY 1 cut(s) 1822
BstHHI GCGC 2 cut(s) 1287, 1821
BstKTI GATC 6 cut(s) 40, 142, 409, 773, 1222, 1676
BstMAI GTCTC 3 cut(s) 896, 1427, 1886
BstMBI GATC 6 cut(s) 37, 139, 406, 770, 1219, 1673
BstNSI RCATGY 4 cut(s) 680, 983, 1212, 1285
BstPAI GACNNNNGTC 1 cut(s) 1086
BstSFI CTRYAG 3 cut(s) 41, 915, 1918
BstUI CGCG 1 cut(s) 973
BstV1I GCAGC 7 cut(s) 900, 1183, 1186, 1429, 1659, 1662, 1896
BstV2I GAAGAC 4 cut(s) 102, 117, 123, 416
BstX2I RGATCY 4 cut(s) 37, 139, 1219, 1673
BstYI RGATCY 4 cut(s) 37, 139, 1219, 1673
Bsu36I CCTNAGG 1 cut(s) 1294
BsuRI GGCC 5 cut(s) 188, 363, 474, 1300, 1453
BtrI CACGTC 1 cut(s) 549
BtsCI GGATG 4 cut(s) 58, 74, 1679, 1855
BtsIMutI CAGTG 3 cut(s) 344, 1377, 1398
BtuMI TCGCGA 1 cut(s) 973
Cac8I GCNNGC 6 cut(s) 235, 272, 633, 981, 1126, 1645
CaiI CAGNNNCTG 1 cut(s) 1301
CciI TCATGA 2 cut(s) 181, 1934
CfoI GCGC 2 cut(s) 1287, 1821
Cfr10I RCCGGY 1 cut(s) 1192
Cfr13I GGNCC 6 cut(s) 173, 789, 1081, 1298, 1451, 1462
CseI GACGC 2 cut(s) 137, 936
Csp6I GTAC 3 cut(s) 680, 1858, 1923
CviAII CATG 9 cut(s) 182, 677, 875, 980, 1209, 1215, 1282, 1625, 1935
CviQI GTAC 3 cut(s) 680, 1858, 1923
DdeI CTNAG 7 cut(s) 100, 242, 327, 826, 1151, 1294, 1886
DpnI GATC 6 cut(s) 39, 141, 408, 772, 1221, 1675
DpnII GATC 6 cut(s) 37, 139, 406, 770, 1219, 1673
DraI TTTAAA 2 cut(s) 1068, 1635
DraIII CACNNNGTG 1 cut(s) 19
Eam1104I CTCTTC 3 cut(s) 42, 1377, 1785
EarI CTCTTC 3 cut(s) 42, 1377, 1785
Ecl136II GAGCTC 1 cut(s) 324
Eco130I CCWWGG 2 cut(s) 1129, 1480
Eco24I GRGCYC 1 cut(s) 326
Eco31I GGTCTC 1 cut(s) 1886
Eco32I GATATC 2 cut(s) 1398, 1699
Eco47I GGWCC 4 cut(s) 173, 789, 1081, 1462
Eco53kI GAGCTC 1 cut(s) 324
Eco57I CTGAAG 2 cut(s) 1334, 1779
Eco72I CACGTG 1 cut(s) 1595
Eco81I CCTNAGG 1 cut(s) 1294
EcoICRI GAGCTC 1 cut(s) 324
EcoO109I RGGNCCY 4 cut(s) 173, 789, 1081, 1298
EcoRV GATATC 2 cut(s) 1398, 1699
EcoT14I CCWWGG 2 cut(s) 1129, 1480
EcoT22I ATGCAT 1 cut(s) 880
EcoT38I GRGCYC 1 cut(s) 326
ErhI CCWWGG 2 cut(s) 1129, 1480
Esp3I CGTCTC 1 cut(s) 896
FaeI CATG 9 cut(s) 185, 680, 878, 983, 1212, 1218, 1285, 1628, 1938
FalI AAGNNNNNCTT 2 cut(s) 990, 1022
FaqI GGGAC 5 cut(s) 186, 684, 802, 1094, 1448
FatI CATG 9 cut(s) 181, 676, 874, 979, 1208, 1214, 1281, 1624, 1934
FauNDI CATATG 1 cut(s) 1033
FbaI TGATCA 1 cut(s) 406
FokI GGATG 4 cut(s) 61, 65, 1666, 1862
FriOI GRGCYC 1 cut(s) 326
FspBI CTAG 4 cut(s) 470, 683, 1512, 1781
GlaI GCGC 2 cut(s) 1286, 1820
GsaI CCCAGC 1 cut(s) 987
HaeII RGCGCY 1 cut(s) 1822
HaeIII GGCC 5 cut(s) 188, 363, 474, 1300, 1453
HapII CCGG 3 cut(s) 315, 1193, 1683
HgaI GACGC 2 cut(s) 137, 936
HhaI GCGC 2 cut(s) 1287, 1821
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 9 cut(s) 185, 680, 878, 983, 1212, 1218, 1285, 1628, 1938
Hin6I GCGC 2 cut(s) 1285, 1819
HinP1I GCGC 2 cut(s) 1285, 1819
HincII GTYRAC 2 cut(s) 568, 714
HindII GTYRAC 2 cut(s) 568, 714
HindIII AAGCTT 1 cut(s) 1928
HpaII CCGG 3 cut(s) 315, 1193, 1683
HphI GGTGA 2 cut(s) 275, 416
Hpy166II GTNNAC 5 cut(s) 434, 568, 596, 714, 1860
Hpy8I GTNNAC 5 cut(s) 434, 568, 596, 714, 1860
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 7 cut(s) 282, 619, 828, 969, 1107, 1309, 1599
HpyCH4III ACNGT 6 cut(s) 404, 1345, 1393, 1615, 1813, 1922
HpyCH4IV ACGT 7 cut(s) 90, 548, 939, 1087, 1170, 1594, 1689
HpyF3I CTNAG 7 cut(s) 100, 242, 327, 826, 1151, 1294, 1886
HpySE526I ACGT 7 cut(s) 90, 548, 939, 1087, 1170, 1594, 1689
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 9 cut(s) 185, 680, 878, 983, 1212, 1218, 1285, 1628, 1938
HspAI GCGC 2 cut(s) 1285, 1819
KflI GGGWCCC 1 cut(s) 173
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 6 cut(s) 37, 139, 406, 770, 1219, 1673
LguI GCTCTTC 1 cut(s) 1785
LmnI GCTCC 1 cut(s) 628
Lsp1109I GCAGC 7 cut(s) 900, 1183, 1186, 1429, 1659, 1662, 1896
LweI GCATC 8 cut(s) 31, 226, 465, 773, 887, 934, 1412, 1763
MaeI CTAG 4 cut(s) 470, 683, 1512, 1781
MaeII ACGT 7 cut(s) 90, 548, 939, 1087, 1170, 1594, 1689
MaeIII GTNAC 3 cut(s) 263, 549, 909
MalI GATC 6 cut(s) 39, 141, 408, 772, 1221, 1675
MbiI CCGCTC 1 cut(s) 246
MboI GATC 6 cut(s) 37, 139, 406, 770, 1219, 1673
MfeI CAATTG 1 cut(s) 1410
MflI RGATCY 4 cut(s) 37, 139, 1219, 1673
MhlI GDGCHC 2 cut(s) 326, 928
MlyI GAGTC 3 cut(s) 92, 194, 719
Mph1103I ATGCAT 1 cut(s) 880
MroXI GAANNNNTTC 1 cut(s) 542
MseI TTAA 4 cut(s) 965, 1067, 1634, 1824
MslI CAYNNNNRTG 1 cut(s) 1213
MspI CCGG 3 cut(s) 315, 1193, 1683
MunI CAATTG 1 cut(s) 1410
Mva1269I GAATGC 1 cut(s) 1348
MvnI CGCG 1 cut(s) 973
NdeI CATATG 1 cut(s) 1033
NdeII GATC 6 cut(s) 37, 139, 406, 770, 1219, 1673
NlaIII CATG 9 cut(s) 185, 680, 878, 983, 1212, 1218, 1285, 1628, 1938
NlaIV GGNNCC 8 cut(s) 141, 174, 175, 790, 1082, 1452, 1464, 1621
NmuCI GTSAC 2 cut(s) 263, 549
NruI TCGCGA 1 cut(s) 973
NsiI ATGCAT 1 cut(s) 880
NspI RCATGY 4 cut(s) 680, 983, 1212, 1285
PaeI GCATGC 1 cut(s) 983
PagI TCATGA 2 cut(s) 181, 1934
PciSI GCTCTTC 1 cut(s) 1785
PctI GAATGC 1 cut(s) 1348
PdmI GAANNNNTTC 1 cut(s) 542
PfeI GAWTC 7 cut(s) 282, 343, 575, 589, 804, 1229, 1307
PleI GAGTC 3 cut(s) 92, 194, 718
PmaCI CACGTG 1 cut(s) 1595
PmlI CACGTG 1 cut(s) 1595
PpsI GAGTC 3 cut(s) 92, 194, 718
Ppu21I YACGTR 1 cut(s) 1595
PpuMI RGGWCCY 3 cut(s) 173, 789, 1081
PshAI GACNNNNGTC 1 cut(s) 1086
Psp124BI GAGCTC 1 cut(s) 326
Psp5II RGGWCCY 3 cut(s) 173, 789, 1081
PspCI CACGTG 1 cut(s) 1595
PspFI CCCAGC 1 cut(s) 983
PspN4I GGNNCC 8 cut(s) 141, 174, 175, 790, 1082, 1452, 1464, 1621
PspPI GGNCC 6 cut(s) 173, 789, 1081, 1298, 1451, 1462
PspPPI RGGWCCY 3 cut(s) 173, 789, 1081
PstNI CAGNNNCTG 1 cut(s) 1301
PsuI RGATCY 4 cut(s) 37, 139, 1219, 1673
RruI TCGCGA 1 cut(s) 973
RsaI GTAC 3 cut(s) 681, 1859, 1924
RsaNI GTAC 3 cut(s) 680, 1858, 1923
RseI CAYNNNNRTG 1 cut(s) 1213
SacI GAGCTC 1 cut(s) 326
SapI GCTCTTC 1 cut(s) 1785
SaqAI TTAA 4 cut(s) 965, 1067, 1634, 1824
Sau3AI GATC 6 cut(s) 37, 139, 406, 770, 1219, 1673
Sau96I GGNCC 6 cut(s) 173, 789, 1081, 1298, 1451, 1462
SchI GAGTC 3 cut(s) 92, 194, 719
SduI GDGCHC 2 cut(s) 326, 928
SfaNI GCATC 8 cut(s) 31, 226, 465, 773, 887, 934, 1412, 1763
SfcI CTRYAG 3 cut(s) 41, 915, 1918
SinI GGWCC 4 cut(s) 173, 789, 1081, 1462
SmiMI CAYNNNNRTG 1 cut(s) 1213
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
SpeI ACTAGT 1 cut(s) 1780
SphI GCATGC 1 cut(s) 983
SspMI CTAG 4 cut(s) 470, 683, 1512, 1781
SstI GAGCTC 1 cut(s) 326
StyI CCWWGG 2 cut(s) 1129, 1480
TaaI ACNGT 6 cut(s) 404, 1345, 1393, 1615, 1813, 1922
TaiI ACGT 7 cut(s) 93, 551, 942, 1090, 1173, 1597, 1692
TaqI TCGA 3 cut(s) 459, 697, 1551
TatI WGTACW 2 cut(s) 679, 1857
TauI GCSGC 6 cut(s) 249, 252, 255, 258, 261, 305
TfiI GAWTC 7 cut(s) 282, 343, 575, 589, 804, 1229, 1307
Tru1I TTAA 4 cut(s) 965, 1067, 1634, 1824
Tru9I TTAA 4 cut(s) 965, 1067, 1634, 1824
TscAI CASTG 3 cut(s) 344, 1384, 1398
TseFI GTSAC 2 cut(s) 263, 549
TseI GCWGC 7 cut(s) 888, 1196, 1199, 1442, 1647, 1650, 1909
Tsp45I GTSAC 2 cut(s) 263, 549
TspDTI ATGAA 7 cut(s) 69, 1046, 1328, 1347, 1356, 1668, 1923
TspGWI ACGGA 3 cut(s) 67, 152, 920
TspRI CASTG 3 cut(s) 344, 1384, 1398
VpaK11BI GGWCC 4 cut(s) 173, 789, 1081, 1462
XapI RAATTY 4 cut(s) 693, 957, 1069, 1429
XceI RCATGY 4 cut(s) 680, 983, 1212, 1285
XmnI GAANNNNTTC 1 cut(s) 542
XspI CTAG 4 cut(s) 470, 683, 1512, 1781
Zsp2I ATGCAT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.