Rroxscaffold_6G00410770

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011045
Physical Location & Seq
Forward (+)
33176050 .. 33181301
5252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410770.1

Sequence Viewer

Length: 1926 bp
ATGGCAGACCCTCACCCTCACCCTCACTATGTGCATCTTGACGATGTAGATCTACAGGAAGAGGATGAAGAAGCCTCAGAAGAACCATCCGTCATCGGCGACGTTGAAGACTCAGAACAAGAAGACGAAGAAGAAGAAGAAGAAGAAGAAGAACCAGACGCCCAAACGGATCCCAATTATGCTCTCTATCTTTCCAGAATGGGACCCACTCATGAGGCCGAAGAAGCCGACTCGGATTTTGAACTGCATCTGGTCAGAACCGCTTGCTTTCTGCGCGGCGGCGGCGGCGGTGACGGTGACGGCAAGCGGAGAAGAATCGGAAGCCAGGAAACGGCGGCGTTTGACACCGGAGAGAGCTCTCAGAGCAGTCAGTGGAATCCGGATGAAATTGATGGCTTGTTTTGCCCAATTTGCTTGGAGGCTTGGACTAATGACGGTGATCACTACATCTGCTGTCTTCCTTGTGGACACATTTTCGGTAGGTCCTGCATCGAAAAATGGCTAGGCCAGAGTAAAAGACAGGGAAAGTGTCCTCAATGCAATGCCAAATGCAAGTTGAACGATGTCAGGAAACTTTTCGCGTCACGAGTTATTTCTGTTGACGAAGAATCACAAAAGAGGATTCGTTCACTTGAGGCTAAATGTGCTTCTTTTGAGAAGGAGCGAGCAGAATGGCATAAGAGGAAAGCAGAATTGCTAAAGGGACAGCGTGTACTAGAACTCAAAATTCAACAACTTATGGAGAGCAAAGCATCAGGATCAATCAATGCTACTGGGGCCTTTGAAAGAAGATTCGTGTCTGGTAATAACATCTTAGAGGGAAGGTCTTATGGGCTAACATTTGGCTCTAATACCTGTGGACAAGCATCTCCTTGCAGCTTCAAATTAGAGAAGGAGTTGCATGTAGCTGGTGCTCATTTGTTTGACGTTGATGCGTCTAACCAAATTTTGTTAATTTCGCGAAGGCATGCTGGGCTAGGTGGAAAAGATGTGCTTACCAAAATGAGCCTGATACCTCCATATGACAGAGATGATATTCTTCTTCCTAGCTCTTTAAATTCTATCAGGGACCTACGTCTTTCTCCTTCTAACACTAACCTTGCACTCTTTACTTGCCTAGGGAAGAAATTGGCAGTTCTCAGTACGGAGAACAACAACGTCATTGTTTCCTATGACCTACCGGCTGCTGCTTGGACATGCTCATGGGATCTCAACGATTCAAATTATATATATGCTGGATTACAGAATGGTTATCTTCTGGTGTTTGACATGCGCCAAACCTCAGGGCCTGTAGAATCCCTGAAGGGGCTGACAAGCAACCCAATTCATACCGTGCATTCCCTTTCATATAATTCAGCTCTTCCCACTGGTGTTAGAACAGTGATATCCGCATCGTCAATTGGCTTATGTCTCTGGAATTTTGGTGGTGCTGCAAAGGGGCCAATTTTGGTCCCTGAAACAGAGAACCAAGGAGTTTGTATATCTCTTGCATATTGCCCTAGAACAGATGACATTGTTGCTTCCTATCGCCCCAAAGTCGAGTTTTCTGATGAGGCAGTTCTTACACAACCAACTCCTTCACGTGCCATCGGACAAGGAACAGTCGGTTCCCATGTTCTTTTAAAGAGAACAGGCAGCAGCAGTCATTTTCTAAAGTTGGGATCTTCATCCGGCAACGTGTGTGATATCGGACTGCCAAAATCTGCAATTATCAACGTAGAAAATTGTGGCAGTTTGTTTGCATCTGAAGATAAACTATCTTCTGAACTAGTTTTAGAAGAGCTGTCGTCTTTTACTACTGTTCAGCGCCTTAACTTACAGCGAAGTCCTGTTCAGGATGTGAAGTACACATCTTTCTTAAGGAAAGGTCTCCTCAGTTGTTTGAATGGTAACACGCTGCAACTCTTCAGTACCAAGCTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

641

Amino Acids

70.28

Weight (kDa)

5.12

Isoelectric Point (pI)

55.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 135 - 181 5.6e-10 Ring finger domain
zf-RING_UBOX PF13445 135 - 178 1e-06 RING-type zinc-finger
WD40_RFWD3 PF23419 353 - 636 1.8e-30 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03450 AT5G03750
fragaria_vesca FvH4_6g16560 FvH4_6g16560
malus_domestica MD12G1109200.v1.1
prunus_persica Prupe.7G127800_v2.0.a1
pyrus_communis pycom12g10940
rosa_chinensis RchiOBHm_Chr3g0470481
rosa_laevigata RLG00000024263
rosa_multiflora Rmu_sc0002115.1_g000004 Rmu_sc0003381.1_g000011
rosa_roxburghii Rroxscaffold_4G00302270 Rroxscaffold_6G00410770
rosa_rugosa Rorug03G0109400 Rorug03G0109500
rosa_samantha Rh3AG159800 Rh3BG184300 Rh3CG174800 Rh3DG190600
rosa_wichuraiana Rw3G015120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 276, 581, 961
AccIII TCCGGA 1 cut(s) 379
AciI CCGC 9 cut(s) 261, 276, 279, 282, 285, 288, 307, 335, 1389
AclWI GGATC 5 cut(s) 164, 177, 766, 1215, 1669
AcsI RAATTY 4 cut(s) 726, 945, 1057, 1417
AcuI CTGAAG 3 cut(s) 1322, 1767, 1891
AcvI CACGTG 1 cut(s) 1583
AcyI GRCGYC 1 cut(s) 159
AdeI CACNNNGTG 1 cut(s) 31
AfaI GTAC 4 cut(s) 714, 1144, 1847, 1912
AfiI CCNNNNNNNGG 2 cut(s) 331, 1305
AflII CTTAAG 1 cut(s) 1858
AflIII ACRYGT 1 cut(s) 1677
AgsI TTSAA 8 cut(s) 107, 242, 559, 731, 785, 883, 1221, 1885
AhlI ACTAGT 1 cut(s) 1768
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 7 cut(s) 357, 879, 908, 1050, 1358, 1783, 1918
AluI AGCT 7 cut(s) 357, 879, 908, 1050, 1358, 1783, 1918
Alw21I GWGCWC 2 cut(s) 359, 916
Alw26I GTCTC 2 cut(s) 1415, 1874
AlwI GGATC 5 cut(s) 164, 177, 766, 1215, 1669
AlwNI CAGNNNCTG 1 cut(s) 1289
Aor13HI TCCGGA 1 cut(s) 379
AoxI GGCC 5 cut(s) 216, 505, 777, 1286, 1439
ApeKI GCWGC 7 cut(s) 876, 1184, 1187, 1430, 1635, 1638, 1897
ApoI RAATTY 4 cut(s) 726, 945, 1057, 1417
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
Asp700I GAANNNNTTC 1 cut(s) 575
AspA2I CCTAGG 1 cut(s) 1117
AspLEI GCGC 3 cut(s) 276, 1275, 1809
AspS9I GGNCC 7 cut(s) 203, 483, 777, 1069, 1286, 1439, 1450
AsuHPI GGTGA 5 cut(s) 5, 11, 302, 308, 449
AvaII GGWCC 4 cut(s) 203, 483, 1069, 1450
AvrII CCTAGG 1 cut(s) 1117
AxyI CCTNAGG 1 cut(s) 1282
BamHI GGATCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 359
BauI CACGAG 1 cut(s) 585
BbrPI CACGTG 1 cut(s) 1583
BbsI GAAGAC 3 cut(s) 114, 129, 449
Bbv12I GWGCWC 2 cut(s) 359, 916
BbvI GCAGC 7 cut(s) 888, 1171, 1174, 1417, 1647, 1650, 1884
BccI CCATC 3 cut(s) 94, 386, 1595
BceAI ACGGC 2 cut(s) 316, 348
BciT130I CCWGG 1 cut(s) 326
BclI TGATCA 1 cut(s) 439
BcoDI GTCTC 2 cut(s) 1415, 1874
BcuI ACTAGT 1 cut(s) 1768
BfaI CTAG 7 cut(s) 503, 716, 977, 1047, 1118, 1500, 1769
BfmI CTRYAG 2 cut(s) 53, 1290
BfoI RGCGCY 1 cut(s) 1810
BfrI CTTAAG 1 cut(s) 1858
BglII AGATCT 1 cut(s) 49
BlnI CCTAGG 1 cut(s) 1117
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 4 cut(s) 203, 483, 1069, 1450
BmgT120I GGNCC 7 cut(s) 203, 483, 777, 1069, 1286, 1439, 1450
BmiI GGNNCC 8 cut(s) 171, 204, 205, 778, 1070, 1440, 1452, 1609
BmrFI CCNGG 1 cut(s) 326
BmrI ACTGGG 1 cut(s) 783
BmsI GCATC 8 cut(s) 43, 256, 498, 761, 875, 922, 1400, 1751
BmuI ACTGGG 1 cut(s) 783
BoxI GACNNNNGTC 1 cut(s) 1074
BpiI GAAGAC 3 cut(s) 114, 129, 449
BpuEI CTTGAG 1 cut(s) 653
BsaAI YACGTR 1 cut(s) 1583
BsaBI GATNNNNATC 2 cut(s) 48, 1666
BsaHI GRCGYC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 1874
BsaJI CCNNGG 2 cut(s) 1117, 1468
BsaWI WCCGGW 2 cut(s) 347, 379
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 1305
Bse118I RCCGGY 1 cut(s) 1180
Bse1I ACTGG 2 cut(s) 778, 1372
Bse21I CCTNAGG 1 cut(s) 1282
Bse3DI GCAATG 1 cut(s) 547
Bse8I GATNNNNATC 2 cut(s) 48, 1666
BseAI TCCGGA 1 cut(s) 379
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 2 cut(s) 1117, 1468
BseGI GGATG 5 cut(s) 70, 86, 388, 1667, 1843
BseJI GATNNNNATC 2 cut(s) 48, 1666
BseLI CCNNNNNNNGG 2 cut(s) 331, 1305
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 6 cut(s) 90, 126, 374, 1153, 1296, 1888
BseNI ACTGG 2 cut(s) 778, 1372
BseRI GAGGAG 1 cut(s) 1862
BseXI GCAGC 7 cut(s) 888, 1171, 1174, 1417, 1647, 1650, 1884
BseYI CCCAGC 1 cut(s) 971
Bsh1236I CGCG 3 cut(s) 276, 581, 961
BshFI GGCC 5 cut(s) 218, 507, 779, 1288, 1441
BsiHKAI GWGCWC 2 cut(s) 359, 916
BsiSI CCGG 4 cut(s) 348, 380, 1181, 1671
BslFI GGGAC 4 cut(s) 216, 717, 1082, 1436
BslI CCNNNNNNNGG 2 cut(s) 331, 1305
BsmAI GTCTC 2 cut(s) 1415, 1874
BsmFI GGGAC 4 cut(s) 216, 717, 1082, 1436
BsmI GAATGC 1 cut(s) 1336
BsnI GGCC 5 cut(s) 218, 507, 779, 1288, 1441
Bso31I GGTCTC 1 cut(s) 1874
Bsp1286I GDGCHC 2 cut(s) 359, 916
Bsp13I TCCGGA 1 cut(s) 379
Bsp143I GATC 6 cut(s) 49, 169, 439, 758, 1207, 1661
Bsp68I TCGCGA 1 cut(s) 961
BspACI CCGC 9 cut(s) 261, 276, 279, 282, 285, 288, 307, 335, 1389
BspANI GGCC 5 cut(s) 218, 507, 779, 1288, 1441
BspCNI CTCAG 6 cut(s) 89, 125, 373, 1152, 1295, 1887
BspEI TCCGGA 1 cut(s) 379
BspFNI CGCG 3 cut(s) 276, 581, 961
BspHI TCATGA 2 cut(s) 211, 1922
BspLI GGNNCC 8 cut(s) 171, 204, 205, 778, 1070, 1440, 1452, 1609
BspPI GGATC 5 cut(s) 164, 177, 766, 1215, 1669
BspQI GCTCTTC 2 cut(s) 1365, 1773
BspTI CTTAAG 1 cut(s) 1858
BspTNI GGTCTC 1 cut(s) 1874
BsrDI GCAATG 1 cut(s) 547
BsrFI RCCGGY 1 cut(s) 1180
BsrI ACTGG 2 cut(s) 778, 1372
BssAI RCCGGY 1 cut(s) 1180
BssECI CCNNGG 2 cut(s) 1117, 1468
BssMI GATC 6 cut(s) 49, 169, 439, 758, 1207, 1661
BssNI GRCGYC 1 cut(s) 159
BssSI CACGAG 1 cut(s) 585
BssT1I CCWWGG 2 cut(s) 1117, 1468
Bst2BI CACGAG 1 cut(s) 585
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 6 cut(s) 296, 437, 1333, 1381, 1603, 1801
Bst6I CTCTTC 4 cut(s) 54, 1365, 1773, 1910
BstACI GRCGYC 1 cut(s) 159
BstAFI CTTAAG 1 cut(s) 1858
BstBAI YACGTR 1 cut(s) 1583
BstC8I GCNNGC 4 cut(s) 265, 305, 666, 969
BstDEI CTNAG 7 cut(s) 76, 112, 360, 814, 1139, 1282, 1874
BstF5I GGATG 5 cut(s) 70, 86, 388, 1667, 1843
BstFNI CGCG 3 cut(s) 276, 581, 961
BstH2I RGCGCY 1 cut(s) 1810
BstHHI GCGC 3 cut(s) 276, 1275, 1809
BstKTI GATC 6 cut(s) 52, 172, 442, 761, 1210, 1664
BstMAI GTCTC 2 cut(s) 1415, 1874
BstMBI GATC 6 cut(s) 49, 169, 439, 758, 1207, 1661
BstNI CCWGG 1 cut(s) 326
BstNSI RCATGY 4 cut(s) 905, 971, 1200, 1273
BstPAI GACNNNNGTC 1 cut(s) 1074
BstSCI CCNGG 1 cut(s) 324
BstSFI CTRYAG 2 cut(s) 53, 1290
BstUI CGCG 3 cut(s) 276, 581, 961
BstV1I GCAGC 7 cut(s) 888, 1171, 1174, 1417, 1647, 1650, 1884
BstV2I GAAGAC 3 cut(s) 114, 129, 449
BstX2I RGATCY 4 cut(s) 49, 169, 1207, 1661
BstYI RGATCY 4 cut(s) 49, 169, 1207, 1661
Bsu36I CCTNAGG 1 cut(s) 1282
BsuRI GGCC 5 cut(s) 218, 507, 779, 1288, 1441
BtsCI GGATG 5 cut(s) 70, 86, 388, 1667, 1843
BtsIMutI CAGTG 3 cut(s) 377, 1365, 1386
BtuMI TCGCGA 1 cut(s) 961
Cac8I GCNNGC 4 cut(s) 265, 305, 666, 969
CaiI CAGNNNCTG 1 cut(s) 1289
CciI TCATGA 2 cut(s) 211, 1922
CfoI GCGC 3 cut(s) 276, 1275, 1809
Cfr10I RCCGGY 1 cut(s) 1180
Cfr13I GGNCC 7 cut(s) 203, 483, 777, 1069, 1286, 1439, 1450
CseI GACGC 3 cut(s) 167, 570, 924
Csp6I GTAC 4 cut(s) 713, 1143, 1846, 1911
CviAII CATG 8 cut(s) 212, 902, 968, 1197, 1203, 1270, 1613, 1923
CviQI GTAC 4 cut(s) 713, 1143, 1846, 1911
DdeI CTNAG 7 cut(s) 76, 112, 360, 814, 1139, 1282, 1874
DpnI GATC 6 cut(s) 51, 171, 441, 760, 1209, 1663
DpnII GATC 6 cut(s) 49, 169, 439, 758, 1207, 1661
DraI TTTAAA 2 cut(s) 1056, 1623
DraIII CACNNNGTG 1 cut(s) 31
Eam1104I CTCTTC 4 cut(s) 54, 1365, 1773, 1910
EarI CTCTTC 4 cut(s) 54, 1365, 1773, 1910
Ecl136II GAGCTC 1 cut(s) 357
Eco130I CCWWGG 2 cut(s) 1117, 1468
Eco24I GRGCYC 1 cut(s) 359
Eco31I GGTCTC 1 cut(s) 1874
Eco32I GATATC 2 cut(s) 1386, 1687
Eco47I GGWCC 4 cut(s) 203, 483, 1069, 1450
Eco53kI GAGCTC 1 cut(s) 357
Eco57I CTGAAG 3 cut(s) 1322, 1767, 1891
Eco72I CACGTG 1 cut(s) 1583
Eco81I CCTNAGG 1 cut(s) 1282
EcoICRI GAGCTC 1 cut(s) 357
EcoO109I RGGNCCY 5 cut(s) 203, 483, 777, 1069, 1286
EcoRII CCWGG 1 cut(s) 324
EcoRV GATATC 2 cut(s) 1386, 1687
EcoT14I CCWWGG 2 cut(s) 1117, 1468
EcoT38I GRGCYC 1 cut(s) 359
ErhI CCWWGG 2 cut(s) 1117, 1468
FaeI CATG 8 cut(s) 215, 905, 971, 1200, 1206, 1273, 1616, 1926
FalI AAGNNNNNCTT 2 cut(s) 978, 1010
FaqI GGGAC 4 cut(s) 216, 717, 1082, 1436
FatI CATG 8 cut(s) 211, 901, 967, 1196, 1202, 1269, 1612, 1922
FauNDI CATATG 1 cut(s) 1021
FbaI TGATCA 1 cut(s) 439
FokI GGATG 5 cut(s) 73, 77, 395, 1654, 1850
FriOI GRGCYC 1 cut(s) 359
FspBI CTAG 7 cut(s) 503, 716, 977, 1047, 1118, 1500, 1769
GlaI GCGC 3 cut(s) 275, 1274, 1808
GsaI CCCAGC 1 cut(s) 975
HaeII RGCGCY 1 cut(s) 1810
HaeIII GGCC 5 cut(s) 218, 507, 779, 1288, 1441
HapII CCGG 4 cut(s) 348, 380, 1181, 1671
HgaI GACGC 3 cut(s) 167, 570, 924
HhaI GCGC 3 cut(s) 276, 1275, 1809
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 8 cut(s) 215, 905, 971, 1200, 1206, 1273, 1616, 1926
Hin6I GCGC 3 cut(s) 274, 1273, 1807
HinP1I GCGC 3 cut(s) 274, 1273, 1807
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 1916
HinfI GANTC 9 cut(s) 110, 230, 315, 376, 608, 622, 792, 1217, 1295
HpaII CCGG 4 cut(s) 348, 380, 1181, 1671
HphI GGTGA 5 cut(s) 5, 11, 302, 308, 449
Hpy166II GTNNAC 6 cut(s) 467, 601, 629, 713, 860, 1848
Hpy8I GTNNAC 6 cut(s) 467, 601, 629, 713, 860, 1848
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 7 cut(s) 652, 816, 886, 957, 1095, 1297, 1587
HpyCH4III ACNGT 6 cut(s) 296, 437, 1333, 1381, 1603, 1801
HpyCH4IV ACGT 7 cut(s) 102, 927, 1075, 1158, 1582, 1677, 1716
HpyF3I CTNAG 7 cut(s) 76, 112, 360, 814, 1139, 1282, 1874
HpySE526I ACGT 7 cut(s) 102, 927, 1075, 1158, 1582, 1677, 1716
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 8 cut(s) 215, 905, 971, 1200, 1206, 1273, 1616, 1926
HspAI GCGC 3 cut(s) 274, 1273, 1807
KflI GGGWCCC 1 cut(s) 203
Kpn2I TCCGGA 1 cut(s) 379
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 6 cut(s) 49, 169, 439, 758, 1207, 1661
LguI GCTCTTC 2 cut(s) 1365, 1773
LmnI GCTCC 1 cut(s) 661
Lsp1109I GCAGC 7 cut(s) 888, 1171, 1174, 1417, 1647, 1650, 1884
LweI GCATC 8 cut(s) 43, 256, 498, 761, 875, 922, 1400, 1751
MaeI CTAG 7 cut(s) 503, 716, 977, 1047, 1118, 1500, 1769
MaeII ACGT 7 cut(s) 102, 927, 1075, 1158, 1582, 1677, 1716
MaeIII GTNAC 4 cut(s) 290, 296, 582, 1889
MalI GATC 6 cut(s) 51, 171, 441, 760, 1209, 1663
MboI GATC 6 cut(s) 49, 169, 439, 758, 1207, 1661
MfeI CAATTG 1 cut(s) 1398
MflI RGATCY 4 cut(s) 49, 169, 1207, 1661
MhlI GDGCHC 2 cut(s) 359, 916
MlyI GAGTC 2 cut(s) 104, 224
MroI TCCGGA 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 575
MseI TTAA 5 cut(s) 953, 1055, 1622, 1812, 1859
MslI CAYNNNNRTG 1 cut(s) 1201
MspCI CTTAAG 1 cut(s) 1858
MspI CCGG 4 cut(s) 348, 380, 1181, 1671
MspR9I CCNGG 1 cut(s) 326
MunI CAATTG 1 cut(s) 1398
Mva1269I GAATGC 1 cut(s) 1336
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 3 cut(s) 276, 581, 961
NdeI CATATG 1 cut(s) 1021
NdeII GATC 6 cut(s) 49, 169, 439, 758, 1207, 1661
NlaIII CATG 8 cut(s) 215, 905, 971, 1200, 1206, 1273, 1616, 1926
NlaIV GGNNCC 8 cut(s) 171, 204, 205, 778, 1070, 1440, 1452, 1609
NmuCI GTSAC 3 cut(s) 290, 296, 582
NruI TCGCGA 1 cut(s) 961
NspI RCATGY 4 cut(s) 905, 971, 1200, 1273
PaeI GCATGC 1 cut(s) 971
PagI TCATGA 2 cut(s) 211, 1922
PciSI GCTCTTC 2 cut(s) 1365, 1773
PctI GAATGC 1 cut(s) 1336
PdmI GAANNNNTTC 1 cut(s) 575
PfeI GAWTC 7 cut(s) 315, 376, 608, 622, 792, 1217, 1295
PleI GAGTC 2 cut(s) 104, 224
PmaCI CACGTG 1 cut(s) 1583
PmlI CACGTG 1 cut(s) 1583
PpsI GAGTC 2 cut(s) 104, 224
Ppu21I YACGTR 1 cut(s) 1583
PpuMI RGGWCCY 3 cut(s) 203, 483, 1069
PshAI GACNNNNGTC 1 cut(s) 1074
Psp124BI GAGCTC 1 cut(s) 359
Psp5II RGGWCCY 3 cut(s) 203, 483, 1069
Psp6I CCWGG 1 cut(s) 324
PspCI CACGTG 1 cut(s) 1583
PspFI CCCAGC 1 cut(s) 971
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 8 cut(s) 171, 204, 205, 778, 1070, 1440, 1452, 1609
PspPI GGNCC 7 cut(s) 203, 483, 777, 1069, 1286, 1439, 1450
PspPPI RGGWCCY 3 cut(s) 203, 483, 1069
PstNI CAGNNNCTG 1 cut(s) 1289
PsuI RGATCY 4 cut(s) 49, 169, 1207, 1661
RruI TCGCGA 1 cut(s) 961
RsaI GTAC 4 cut(s) 714, 1144, 1847, 1912
RsaNI GTAC 4 cut(s) 713, 1143, 1846, 1911
RseI CAYNNNNRTG 1 cut(s) 1201
SacI GAGCTC 1 cut(s) 359
SapI GCTCTTC 2 cut(s) 1365, 1773
SaqAI TTAA 5 cut(s) 953, 1055, 1622, 1812, 1859
Sau3AI GATC 6 cut(s) 49, 169, 439, 758, 1207, 1661
Sau96I GGNCC 7 cut(s) 203, 483, 777, 1069, 1286, 1439, 1450
SchI GAGTC 2 cut(s) 104, 224
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 2 cut(s) 359, 916
SfaNI GCATC 8 cut(s) 43, 256, 498, 761, 875, 922, 1400, 1751
SfcI CTRYAG 2 cut(s) 53, 1290
SinI GGWCC 4 cut(s) 203, 483, 1069, 1450
SmiMI CAYNNNNRTG 1 cut(s) 1201
SmlI CTYRAG 2 cut(s) 632, 1858
SmoI CTYRAG 2 cut(s) 632, 1858
SpeI ACTAGT 1 cut(s) 1768
SphI GCATGC 1 cut(s) 971
SsiI CCGC 9 cut(s) 261, 276, 279, 282, 285, 288, 307, 335, 1389
SspMI CTAG 7 cut(s) 503, 716, 977, 1047, 1118, 1500, 1769
SstI GAGCTC 1 cut(s) 359
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 2 cut(s) 1117, 1468
TaaI ACNGT 6 cut(s) 296, 437, 1333, 1381, 1603, 1801
TaiI ACGT 7 cut(s) 105, 930, 1078, 1161, 1585, 1680, 1719
TaqI TCGA 2 cut(s) 492, 1539
TatI WGTACW 2 cut(s) 712, 1845
TauI GCSGC 5 cut(s) 279, 282, 285, 288, 338
TfiI GAWTC 7 cut(s) 315, 376, 608, 622, 792, 1217, 1295
Tru1I TTAA 5 cut(s) 953, 1055, 1622, 1812, 1859
Tru9I TTAA 5 cut(s) 953, 1055, 1622, 1812, 1859
TscAI CASTG 3 cut(s) 377, 1372, 1386
TseFI GTSAC 3 cut(s) 290, 296, 582
TseI GCWGC 7 cut(s) 876, 1184, 1187, 1430, 1635, 1638, 1897
Tsp45I GTSAC 3 cut(s) 290, 296, 582
TspDTI ATGAA 6 cut(s) 81, 399, 1316, 1335, 1656, 1911
TspGWI ACGGA 3 cut(s) 79, 182, 1160
TspRI CASTG 3 cut(s) 377, 1372, 1386
Vha464I CTTAAG 1 cut(s) 1858
VpaK11BI GGWCC 4 cut(s) 203, 483, 1069, 1450
XapI RAATTY 4 cut(s) 726, 945, 1057, 1417
XceI RCATGY 4 cut(s) 905, 971, 1200, 1273
XmaJI CCTAGG 1 cut(s) 1117
XmnI GAANNNNTTC 1 cut(s) 575
XspI CTAG 7 cut(s) 503, 716, 977, 1047, 1118, 1500, 1769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.