MD12G1109200.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011045
Physical Location & Seq
Forward (+)
17292507 .. 17298100
5594 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1109200.v1.1.491

Sequence Viewer

Length: 1878 bp
ATGGCAAACCCTATCCTATCCTTCATTGATCTTGTTGACGGAGGACTCAGCGAGGAATCCGATGAAGACGAATCTGAAGAAGAAGAAGAAGAAGACGAGGAAGAAGAAGGCACAGTGGACGTTGATTCTGCTGTTTACCTTGCAGATCAAACCGGTGCAGCCGGAAGTGCTCCTGAAACGGCGGCGGCGAGGGATGGGGATGAGGAAGTTGGAAATAAGCGTAGAAGGAGAGGAGGAAGCGAAGCGGTGTCGGGGGATGGAGTTGGCAGTGCAGAGTGCTCCCAAGGCAGCAGCAGCCAATGGAATCGGAACGAAATGGACGGGCTATTTTGCTCTATCTGCTTGGAGCCTTGGACCAATGACGGTGATCATCATATCAGTTGTCTTCCTTGTGGACACCTGTTCGGCATGTCTTGCATAAAACGATGGCTACAGCAGCGTAGCAATTCGAGGAAGTGTCCTCAATGCAACGAAAAATGCATGTTGAAGGATGTTAGGAAGCTTTTTGTATCCCAAATTGTTTCTGTTGATGAAGAGTCACAAAAGAGAATTCGGTCACTTGAGGCTAAATGTGATTCTCTTGAGAAGAAGGAATCTGCTTGGTGTAAGAGAGAAGTTCAATGGAGAAAGCGAGAGCTTGAACTGCAAGATAAAGTTCAACATCTTACAGAGAGGGCAACTTATTTGGAGCATCTATTAGGAGACACACAGAACGGAACATCTGGAGTAGTCAATGCTAATGGGGTTCGTGAAGGAAGATTTGTGGCTGGGCGAAACTTTGGATCAAAGTTTTGTGGACAAGCATATTCTTGCAATTTCATATTAGAGAAGGAGTTGCAGGTAGATGGTGCTCGTTTATTTGATGTTGATGAATCTAACCAAATTATTTTGATTTCGCGAAGGCTGGATGGGATAGGGGGAAGACATGTGCTTACCAAGATGAGTTTGATACCTCCGTATGACAGAGATGATATTCTCCTTCCTAGAGATACAAGAGCCATTAGGGATCTACGCATTTCTTCTTCTAACAGTGACCTTGCACTCTTTGCTTGCTTGGGGAAGAAATTGTCAGTTCTCAGTATGGAAAGCAACAATGTCATCCTTACCTATGATCTACCGGCTGCTGCTTGGACATGTTCATGGGATCTCAACAATCCATATTATGCTTATGCTGGTTTACAGAATGGCTCTCTTTGGGTGTTTGACATGCGCCAAACTGCAGGGCCTGTTGAATCTCGGAGAGGGCTTTCAAACAACCCAATTCATACAGTACATTCCCTTTCACATAATTCAGCTCTTCCTTCTGGTGCTAGATCGGTGCTCTCCGCTTCTTCGATTGGCTTATGTCTGTGGAACTTTGGTTGTGCCGAAGAAGGGCCGACATTAGTCCCTCAAACCGAGAATCAAGGAGTTTGCATATCATTGGCGTATTGTCCTAGCAATGATGACATTGTTGCTTCCTACCGTCCCAAAGTTGAAACGTCTGATGAGACGCTCTTGTCTCAACCACAGCTTACTCCTTCCCGTGGTGGCTTTGGACAAGGAACAGTGGGTTCCCATGTTCTCTTGAAGAGAGCAGACATCAACTGTTTTGATCGTTTGGGTTCTGCATGCACCAATGTATGTGATATCGGGCTTCCAAGATCTGCGATCATCGACGTTGGAAATTATAGCACATTGTTTGCCTCTCAGGATAAGTTGAGTTCTGAATTAGTCTTACAAGAGCTACCGTCTTTTACGACCTTACAGCGCCTTAAATTACAGAAAGATCCTCTTCGTGATCTGAAGTACAGTCCGGCGATAAAACAAGGTCTACTTAGTTGTTTAAGTGGTGACAAACTGCAACTCTTCAGTACCAAGCTTTCAGGAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

626

Amino Acids

68.76

Weight (kDa)

5.12

Isoelectric Point (pI)

59.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 111 - 157 5.3e-09 Ring finger domain
zf-RING_UBOX PF13445 111 - 154 1.3e-06 RING-type zinc-finger
zf-C3HC4 PF00097 111 - 156 2.4e-06 Zinc finger, C3HC4 type (RING finger)
WD40_RFWD3 PF23419 280 - 617 2.2e-29 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03450 AT5G03750
fragaria_vesca FvH4_6g16560 FvH4_6g16560
malus_domestica MD12G1109200.v1.1
prunus_persica Prupe.7G127800_v2.0.a1
pyrus_communis pycom12g10940
rosa_chinensis RchiOBHm_Chr3g0470481
rosa_laevigata RLG00000024263
rosa_multiflora Rmu_sc0002115.1_g000004 Rmu_sc0003381.1_g000011
rosa_roxburghii Rroxscaffold_4G00302270 Rroxscaffold_6G00410770
rosa_rugosa Rorug03G0109400 Rorug03G0109500
rosa_samantha Rh3AG159800 Rh3BG184300 Rh3CG174800 Rh3DG190600
rosa_wichuraiana Rw3G015120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1498
Acc36I ACCTGC 1 cut(s) 829
AccI GTMKAC 1 cut(s) 1813
AccII CGCG 1 cut(s) 898
AciI CCGC 4 cut(s) 182, 185, 245, 1326
AclWI GGATC 4 cut(s) 790, 1014, 1152, 1763
AcsI RAATTY 1 cut(s) 549
AcuI CTGAAG 3 cut(s) 96, 1805, 1834
AfaI GTAC 3 cut(s) 1272, 1790, 1855
AfiI CCNNNNNNNGG 2 cut(s) 1374, 1526
AflIII ACRYGT 2 cut(s) 925, 1133
AgeI ACCGGT 1 cut(s) 152
AgsI TTSAA 8 cut(s) 487, 620, 641, 659, 1232, 1251, 1478, 1570
AluBI AGCT 6 cut(s) 502, 637, 1295, 1513, 1726, 1861
AluI AGCT 6 cut(s) 502, 637, 1295, 1513, 1726, 1861
Alw21I GWGCWC 4 cut(s) 172, 281, 853, 1323
Alw26I GTCTC 3 cut(s) 696, 1484, 1506
AlwI GGATC 4 cut(s) 790, 1014, 1152, 1763
AlwNI CAGNNNCTG 1 cut(s) 1226
AoxI GGCC 2 cut(s) 1223, 1376
ApeKI GCWGC 7 cut(s) 158, 288, 291, 294, 436, 1121, 1124
ApoI RAATTY 1 cut(s) 549
AsiGI ACCGGT 1 cut(s) 152
AspLEI GCGC 2 cut(s) 1212, 1752
AspS9I GGNCC 3 cut(s) 354, 1223, 1376
AsuHPI GGTGA 2 cut(s) 377, 1844
AvaII GGWCC 1 cut(s) 354
BaeI ACNNNNGTAYC 2 cut(s) 941, 974
BbsI GAAGAC 4 cut(s) 72, 99, 377, 928
Bbv12I GWGCWC 4 cut(s) 172, 281, 853, 1323
BbvI GCAGC 7 cut(s) 170, 300, 303, 306, 448, 1108, 1111
BccI CCATC 5 cut(s) 188, 251, 420, 839, 902
BceAI ACGGC 1 cut(s) 195
BciVI GTATCC 1 cut(s) 520
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 3 cut(s) 696, 1484, 1506
BfaI CTAG 3 cut(s) 984, 1311, 1437
BfmI CTRYAG 2 cut(s) 431, 1218
BfoI RGCGCY 1 cut(s) 1753
BfuAI ACCTGC 1 cut(s) 829
BfuI GTATCC 1 cut(s) 520
BglII AGATCT 1 cut(s) 1643
BisI GCNGC 9 cut(s) 159, 183, 186, 289, 292, 295, 437, 1122, 1125
BlsI GCNGC 9 cut(s) 160, 184, 187, 290, 293, 296, 438, 1123, 1126
Bme18I GGWCC 1 cut(s) 354
BmgT120I GGNCC 3 cut(s) 354, 1223, 1376
BmiI GGNNCC 2 cut(s) 348, 1555
BmsI GCATC 1 cut(s) 700
BoxI GACNNNNGTC 1 cut(s) 1385
BpiI GAAGAC 4 cut(s) 72, 99, 377, 928
BpmI CTGGAG 1 cut(s) 744
BpuEI CTTGAG 2 cut(s) 581, 602
BsaJI CCNNGG 3 cut(s) 283, 350, 1525
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 2 cut(s) 1374, 1526
Bse118I RCCGGY 2 cut(s) 152, 1117
Bse3DI GCAATG 1 cut(s) 1447
BseDI CCNNGG 3 cut(s) 283, 350, 1525
BseGI GGATG 6 cut(s) 199, 205, 262, 496, 913, 1098
BseLI CCNNNNNNNGG 2 cut(s) 1374, 1526
BseMI GCAATG 1 cut(s) 1447
BseMII CTCAG 3 cut(s) 61, 1090, 1703
BseRI GAGGAG 1 cut(s) 246
BseXI GCAGC 7 cut(s) 170, 300, 303, 306, 448, 1108, 1111
BseYI CCCAGC 1 cut(s) 767
BsgI GTGCAG 2 cut(s) 177, 291
Bsh1236I CGCG 1 cut(s) 898
BshFI GGCC 2 cut(s) 1225, 1378
BshTI ACCGGT 1 cut(s) 152
BsiHKAI GWGCWC 4 cut(s) 172, 281, 853, 1323
BsiSI CCGG 4 cut(s) 153, 162, 1118, 1796
BslFI GGGAC 2 cut(s) 1373, 1452
BslI CCNNNNNNNGG 2 cut(s) 1374, 1526
BsmAI GTCTC 3 cut(s) 696, 1484, 1506
BsmBI CGTCTC 1 cut(s) 1484
BsmFI GGGAC 2 cut(s) 1373, 1452
BsnI GGCC 2 cut(s) 1225, 1378
Bsp1286I GDGCHC 4 cut(s) 172, 281, 853, 1323
Bsp68I TCGCGA 1 cut(s) 898
BspACI CCGC 4 cut(s) 182, 185, 245, 1326
BspANI GGCC 2 cut(s) 1225, 1378
BspCNI CTCAG 3 cut(s) 60, 1089, 1702
BspFNI CGCG 1 cut(s) 898
BspLI GGNNCC 2 cut(s) 348, 1555
BspMAI CTGCAG 1 cut(s) 1222
BspMI ACCTGC 1 cut(s) 829
BspPI GGATC 4 cut(s) 790, 1014, 1152, 1763
BspQI GCTCTTC 1 cut(s) 1302
BsrDI GCAATG 1 cut(s) 1447
BsrFI RCCGGY 2 cut(s) 152, 1117
BssAI RCCGGY 2 cut(s) 152, 1117
BssECI CCNNGG 3 cut(s) 283, 350, 1525
BssT1I CCWWGG 2 cut(s) 283, 350
Bst4CI ACNGT 9 cut(s) 115, 365, 1031, 1270, 1466, 1549, 1589, 1731, 1793
Bst6I CTCTTC 5 cut(s) 528, 1302, 1565, 1779, 1853
BstAPI GCANNNNNTGC 2 cut(s) 414, 1046
BstC8I GCNNGC 2 cut(s) 1051, 1612
BstDEI CTNAG 4 cut(s) 47, 1076, 1689, 1817
BstDSI CCRYGG 1 cut(s) 1525
BstF5I GGATG 6 cut(s) 199, 205, 262, 496, 913, 1098
BstFNI CGCG 1 cut(s) 898
BstH2I RGCGCY 1 cut(s) 1753
BstHHI GCGC 2 cut(s) 1212, 1752
BstMAI GTCTC 3 cut(s) 696, 1484, 1506
BstMWI GCNNNNNNNGC 8 cut(s) 167, 285, 294, 339, 414, 436, 643, 1046
BstNSI RCATGY 6 cut(s) 412, 484, 929, 1137, 1210, 1614
BstPAI GACNNNNGTC 1 cut(s) 1385
BstSFI CTRYAG 2 cut(s) 431, 1218
BstUI CGCG 1 cut(s) 898
BstV1I GCAGC 7 cut(s) 170, 300, 303, 306, 448, 1108, 1111
BstV2I GAAGAC 4 cut(s) 72, 99, 377, 928
BstX2I RGATCY 4 cut(s) 1006, 1144, 1643, 1768
BstYI RGATCY 4 cut(s) 1006, 1144, 1643, 1768
BsuI GTATCC 1 cut(s) 520
BsuRI GGCC 2 cut(s) 1225, 1378
BtgI CCRYGG 1 cut(s) 1525
BtsCI GGATG 6 cut(s) 199, 205, 262, 496, 913, 1098
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 4 cut(s) 120, 274, 1036, 1554
BtuMI TCGCGA 1 cut(s) 898
BveI ACCTGC 1 cut(s) 829
Cac8I GCNNGC 2 cut(s) 1051, 1612
CaiI CAGNNNCTG 1 cut(s) 1226
CfoI GCGC 2 cut(s) 1212, 1752
Cfr10I RCCGGY 2 cut(s) 152, 1117
Cfr13I GGNCC 3 cut(s) 354, 1223, 1376
CseI GACGC 1 cut(s) 1501
Csp6I GTAC 3 cut(s) 1271, 1789, 1854
CspAI ACCGGT 1 cut(s) 152
CviAII CATG 8 cut(s) 409, 481, 926, 1134, 1140, 1207, 1559, 1611
CviQI GTAC 3 cut(s) 1271, 1789, 1854
DdeI CTNAG 4 cut(s) 47, 1076, 1689, 1817
DrdI GACNNNNNNGTC 1 cut(s) 1498
DseDI GACNNNNNNGTC 1 cut(s) 1498
Eam1104I CTCTTC 5 cut(s) 528, 1302, 1565, 1779, 1853
EarI CTCTTC 5 cut(s) 528, 1302, 1565, 1779, 1853
Eco130I CCWWGG 2 cut(s) 283, 350
Eco32I GATATC 1 cut(s) 1630
Eco47I GGWCC 1 cut(s) 354
Eco57I CTGAAG 3 cut(s) 96, 1805, 1834
EcoO109I RGGNCCY 1 cut(s) 1223
EcoRI GAATTC 1 cut(s) 549
EcoRV GATATC 1 cut(s) 1630
EcoT14I CCWWGG 2 cut(s) 283, 350
EcoT22I ATGCAT 1 cut(s) 482
ErhI CCWWGG 2 cut(s) 283, 350
Esp3I CGTCTC 1 cut(s) 1484
FaeI CATG 8 cut(s) 412, 484, 929, 1137, 1143, 1210, 1562, 1614
FalI AAGNNNNNCTT 4 cut(s) 1758, 1790, 1800, 1832
FaqI GGGAC 2 cut(s) 1373, 1452
FatI CATG 8 cut(s) 408, 480, 925, 1133, 1139, 1206, 1558, 1610
FbaI TGATCA 1 cut(s) 367
FblI GTMKAC 1 cut(s) 1813
Fnu4HI GCNGC 9 cut(s) 159, 183, 186, 289, 292, 295, 437, 1122, 1125
FokI GGATG 6 cut(s) 206, 212, 269, 503, 920, 1085
Fsp4HI GCNGC 9 cut(s) 159, 183, 186, 289, 292, 295, 437, 1122, 1125
FspBI CTAG 3 cut(s) 984, 1311, 1437
GlaI GCGC 2 cut(s) 1211, 1751
GluI GCNGC 9 cut(s) 159, 183, 186, 289, 292, 295, 437, 1122, 1125
GsaI CCCAGC 1 cut(s) 771
GsuI CTGGAG 1 cut(s) 744
HaeII RGCGCY 1 cut(s) 1753
HaeIII GGCC 2 cut(s) 1225, 1378
HapII CCGG 4 cut(s) 153, 162, 1118, 1796
HgaI GACGC 1 cut(s) 1501
HhaI GCGC 2 cut(s) 1212, 1752
Hin1II CATG 8 cut(s) 412, 484, 929, 1137, 1143, 1210, 1562, 1614
Hin6I GCGC 2 cut(s) 1210, 1750
HinP1I GCGC 2 cut(s) 1210, 1750
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HindIII AAGCTT 2 cut(s) 500, 1859
HpaII CCGG 4 cut(s) 153, 162, 1118, 1796
HphI GGTGA 2 cut(s) 377, 1844
Hpy166II GTNNAC 7 cut(s) 37, 118, 136, 395, 797, 1178, 1814
Hpy188I TCNGA 7 cut(s) 61, 76, 309, 1239, 1486, 1708, 1785
Hpy188III TCNNGA 9 cut(s) 173, 581, 723, 749, 897, 1567, 1691, 1778, 1866
Hpy8I GTNNAC 7 cut(s) 37, 118, 136, 395, 797, 1178, 1814
Hpy99I CGWCG 1 cut(s) 1661
HpyCH4III ACNGT 9 cut(s) 115, 365, 1031, 1270, 1466, 1549, 1589, 1731, 1793
HpyCH4IV ACGT 3 cut(s) 120, 1481, 1659
HpyF10VI GCNNNNNNNGC 8 cut(s) 167, 285, 294, 339, 414, 436, 643, 1046
HpyF3I CTNAG 4 cut(s) 47, 1076, 1689, 1817
HpySE526I ACGT 3 cut(s) 120, 1481, 1659
Hsp92II CATG 8 cut(s) 412, 484, 929, 1137, 1143, 1210, 1562, 1614
HspAI GCGC 2 cut(s) 1210, 1750
Ksp22I TGATCA 1 cut(s) 367
LguI GCTCTTC 1 cut(s) 1302
LmnI GCTCC 4 cut(s) 175, 284, 346, 688
Lsp1109I GCAGC 7 cut(s) 170, 300, 303, 306, 448, 1108, 1111
LweI GCATC 1 cut(s) 700
MaeI CTAG 3 cut(s) 984, 1311, 1437
MaeII ACGT 3 cut(s) 120, 1481, 1659
MaeIII GTNAC 4 cut(s) 537, 555, 1031, 1832
MflI RGATCY 4 cut(s) 1006, 1144, 1643, 1768
MhlI GDGCHC 4 cut(s) 172, 281, 853, 1323
MlyI GAGTC 2 cut(s) 39, 545
MmeI TCCRAC 2 cut(s) 190, 1642
Mph1103I ATGCAT 1 cut(s) 482
MseI TTAA 2 cut(s) 1755, 1826
MslI CAYNNNNRTG 1 cut(s) 1138
MspI CCGG 4 cut(s) 153, 162, 1118, 1796
MvnI CGCG 1 cut(s) 898
MwoI GCNNNNNNNGC 8 cut(s) 167, 285, 294, 339, 414, 436, 643, 1046
NlaIII CATG 8 cut(s) 412, 484, 929, 1137, 1143, 1210, 1562, 1614
NlaIV GGNNCC 2 cut(s) 348, 1555
NmuCI GTSAC 4 cut(s) 537, 555, 1031, 1832
NruI TCGCGA 1 cut(s) 898
NsiI ATGCAT 1 cut(s) 482
NspI RCATGY 6 cut(s) 412, 484, 929, 1137, 1210, 1614
PaeI GCATGC 1 cut(s) 1614
PciI ACATGT 2 cut(s) 925, 1133
PciSI GCTCTTC 1 cut(s) 1302
PcsI WCGNNNNNNNCGW 1 cut(s) 185
PfeI GAWTC 9 cut(s) 56, 71, 125, 304, 575, 593, 872, 1232, 1402
PinAI ACCGGT 1 cut(s) 152
PkrI GCNGC 9 cut(s) 160, 184, 187, 290, 293, 296, 438, 1123, 1126
PleI GAGTC 2 cut(s) 39, 544
PpsI GAGTC 2 cut(s) 39, 544
PscI ACATGT 2 cut(s) 925, 1133
PshAI GACNNNNGTC 1 cut(s) 1385
PspFI CCCAGC 1 cut(s) 767
PspN4I GGNNCC 2 cut(s) 348, 1555
PspPI GGNCC 3 cut(s) 354, 1223, 1376
PstI CTGCAG 1 cut(s) 1222
PstNI CAGNNNCTG 1 cut(s) 1226
PsuI RGATCY 4 cut(s) 1006, 1144, 1643, 1768
RruI TCGCGA 1 cut(s) 898
RsaI GTAC 3 cut(s) 1272, 1790, 1855
RsaNI GTAC 3 cut(s) 1271, 1789, 1854
RseI CAYNNNNRTG 1 cut(s) 1138
SapI GCTCTTC 1 cut(s) 1302
SaqAI TTAA 2 cut(s) 1755, 1826
SatI GCNGC 9 cut(s) 159, 183, 186, 289, 292, 295, 437, 1122, 1125
Sau96I GGNCC 3 cut(s) 354, 1223, 1376
SchI GAGTC 2 cut(s) 39, 545
SduI GDGCHC 4 cut(s) 172, 281, 853, 1323
SfaNI GCATC 1 cut(s) 700
SfcI CTRYAG 2 cut(s) 431, 1218
SinI GGWCC 1 cut(s) 354
SmiMI CAYNNNNRTG 1 cut(s) 1138
SmlI CTYRAG 2 cut(s) 560, 581
SmoI CTYRAG 2 cut(s) 560, 581
SphI GCATGC 1 cut(s) 1614
SsiI CCGC 4 cut(s) 182, 185, 245, 1326
SspMI CTAG 3 cut(s) 984, 1311, 1437
StyI CCWWGG 2 cut(s) 283, 350
TaaI ACNGT 9 cut(s) 115, 365, 1031, 1270, 1466, 1549, 1589, 1731, 1793
TaiI ACGT 3 cut(s) 123, 1484, 1662
TaqI TCGA 3 cut(s) 449, 1334, 1656
TaqII GACCGA 1 cut(s) 543
TatI WGTACW 2 cut(s) 1270, 1788
TauI GCSGC 2 cut(s) 185, 188
TfiI GAWTC 9 cut(s) 56, 71, 125, 304, 575, 593, 872, 1232, 1402
Tru1I TTAA 2 cut(s) 1755, 1826
Tru9I TTAA 2 cut(s) 1755, 1826
TscAI CASTG 4 cut(s) 120, 274, 1036, 1554
TseFI GTSAC 4 cut(s) 537, 555, 1031, 1832
TseI GCWGC 7 cut(s) 158, 288, 291, 294, 436, 1121, 1124
Tsp45I GTSAC 4 cut(s) 537, 555, 1031, 1832
TspDTI ATGAA 7 cut(s) 13, 78, 546, 808, 885, 1128, 1253
TspGWI ACGGA 3 cut(s) 54, 729, 945
TspRI CASTG 4 cut(s) 120, 274, 1036, 1554
VpaK11BI GGWCC 1 cut(s) 354
XapI RAATTY 1 cut(s) 549
XceI RCATGY 6 cut(s) 412, 484, 929, 1137, 1210, 1614
XmiI GTMKAC 1 cut(s) 1813
XspI CTAG 3 cut(s) 984, 1311, 1437
Zsp2I ATGCAT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.