FvH4_6g16560

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011045
Physical Location & Seq
Reverse (-)
10490338 .. 10495366
5029 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16560.t1

Sequence Viewer

Length: 1857 bp
ATGGCGGACCCTCATCCTCACTACGTCGATCTCGACGATGCAGAGTCCACCGAAGACGACGAAGCCTCAGAAGAACCATCCGTCCCCAGCGACGTCGAAGACTCGGAACAAGACGAAGAAGAAGAAGAACCAGACGTCCAAACCAATTCCAATTACGCTATCTATCTCTCCAGAATGGGTCCCCGTGATGAGCCCAGTGAACCGGAGTCGGATTTCAAACCAGGAAACGGCGGAAAGCGGAGAAAGATCGGGAGCCAGGAAACGGCGGCGTTTGAGACCGGAGAGAGCTCGCAGACTAGTCAGTGGAATCCGAATGAAATTGATGGCCTCTTTTGCCCGATTTGCTTGGAGGCTTGGACTAGTGATGGTGATCACTACATCTGCTGTCTTCCTTGTGGCCACATTTTCGGTAACTCGTGCATAAAAAAATGGATAGGCCAGTCTCAAAAGCAAAAGTGTCCTCAATGCAATGCCAAATGCAAGTGGAAAGATGTTAGGAAGCTTTTCGCGTCTCGAGTTATTGCTGTTGATGAAGAATCACAGAAGAGGATTCGTTCACTTGAGGGTAAATGTGCTTCTCTTGAGAAGGAGCGAGCAGAGTGGCATAAGAAGGAAGCTGAATTGCTAAAGGCACAGGGTGTACTAGAACGGAAAATTCAGCAACTTATGGAGAGCAAAACATCAGAATATACTACTGGGACCTTTGAAAGGAGATTTGGGTCTGGAAATAACATCATAGCTGCAAGGTCTTATGGGGAAACATTTGGCTCTAACCTCTGTGGACAAGCATCTATGTGCAGCTTCAAATTAGAGAAGGAGTTGTGTGTAGCTGGTGCTCGTGTGTTTGACATCGACGCGTCTAACCAAATTTTGTTAATTGCGCGAAGACATGCTGGAATAGGTGGAAAAGATGTGCTTACCAAAATGAGCCTGATACCTCCATATGACAGAGATGATGTTGTTCTTCCTAGCTCTATAAGTTCTATCAGGGACCTGCGTATTTCTCCCTCTAATACTAACCTTGCTCTCTTCGCTTGCCTAGGGAAGAAATTGGTAGTTCTCAGTATGGAGAACAACAACATCATAGTTTCCTATGACCTACCGGCTGCTGCTTGGACATGCTCATGGGATCTCAACGATTCAAATTATATATATGCTGGATTACAGAATGGCTATCTTCTGGTGTTTGACATGCGCCAAACCTCAGGGCCTGTTGAATCCCTGGAGGGGCTGACAAACAACCCAATTCATACTGTGCATTCTCTTTCATCTAATACAGCTTTTCCCTTTTGTGTTAGAACAGTGATATCCGCATCAGCAATTGGCTTATGTCTGTGGCGTTTTGGTGGTGCTGTAACAGAGGGGCCGATTTTGGTCCCCGAAACAGAGAACCAAGGAGTTTGTATATCTCTTGCCTATTGCCCTAGAACAGATGACATTGTTGCTTCCTATCGCCCCAAAGTTGAATTTTCTGATGAGACAGCCTTTACACAACCAATGCTTACTCCTTCACGTGCCATCGGACAAGGAACAGTCGGTTCCCATGTTCTTTTAAAGAGATCAGGCAACAACAGTCATTTTCTGAAGTTGGGATCTTCAGTGGGCAACGTGTGTGATATCGGATTGCCAAAGTCCGCAATTATCAACATAGAAAATCGTGGCAGGTTGTTTGCATCTGAAGATAAACTATCTTCTGAACTAGTTTTAAAGGAGCTGTCGCCTTTTACCACTGTTCAGCGCCTTAACTTACAGCAAAGTCCTGTTCATGATGTGAAGTATACAGCTTCGTCAAGGAAAGGTCTCCTCAGTTGTTTGAATGGTGACATGCTGCAACTCTTCAGTACCGAGCTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

619

Amino Acids

68.08

Weight (kDa)

5.16

Isoelectric Point (pI)

52.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 112 - 157 6.9e-09 Ring finger domain
WD40_RFWD3 PF23419 278 - 613 2.1e-32 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03450 AT5G03750
fragaria_vesca FvH4_6g16560 FvH4_6g16560
malus_domestica MD12G1109200.v1.1
prunus_persica Prupe.7G127800_v2.0.a1
pyrus_communis pycom12g10940
rosa_chinensis RchiOBHm_Chr3g0470481
rosa_laevigata RLG00000024263
rosa_multiflora Rmu_sc0002115.1_g000004 Rmu_sc0003381.1_g000011
rosa_roxburghii Rroxscaffold_4G00302270 Rroxscaffold_6G00410770
rosa_rugosa Rorug03G0109400 Rorug03G0109500
rosa_samantha Rh3AG159800 Rh3BG184300 Rh3CG174800 Rh3DG190600
rosa_wichuraiana Rw3G015120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 96, 138
Acc36I ACCTGC 2 cut(s) 1002, 1653
AccI GTMKAC 1 cut(s) 1778
AccII CGCG 3 cut(s) 509, 857, 883
AciI CCGC 6 cut(s) 5, 231, 238, 266, 1311, 1635
AclWI GGATC 2 cut(s) 1137, 1600
AcoI YGGCCR 1 cut(s) 397
AcsI RAATTY 3 cut(s) 654, 867, 1466
AcuI CTGAAG 4 cut(s) 1581, 1604, 1698, 1822
AcvI CACGTG 1 cut(s) 1514
AcyI GRCGYC 2 cut(s) 93, 135
AdeI CACNNNGTG 1 cut(s) 638
AfaI GTAC 2 cut(s) 642, 1843
AfiI CCNNNNNNNGG 4 cut(s) 227, 262, 708, 1227
AflIII ACRYGT 2 cut(s) 855, 1608
AgsI TTSAA 7 cut(s) 217, 707, 805, 1143, 1217, 1466, 1816
AhlI ACTAGT 3 cut(s) 296, 359, 1699
AjnI CCWGG 3 cut(s) 220, 255, 1221
AjuI GAANNNNNNNTTGG 2 cut(s) 699, 731
AloI GAACNNNNNNTCC 4 cut(s) 66, 98, 120, 152
Alw21I GWGCWC 2 cut(s) 290, 838
Alw26I GTCTC 5 cut(s) 269, 447, 516, 1472, 1805
AlwI GGATC 2 cut(s) 1137, 1600
AlwNI CAGNNNCTG 1 cut(s) 1211
Ama87I CYCGRG 1 cut(s) 513
AoxI GGCC 5 cut(s) 325, 397, 436, 1208, 1364
ApeKI GCWGC 5 cut(s) 740, 798, 1106, 1109, 1828
ApoI RAATTY 3 cut(s) 654, 867, 1466
Asp700I GAANNNNTTC 1 cut(s) 503
AspA2I CCTAGG 1 cut(s) 1039
AspLEI GCGC 3 cut(s) 883, 1197, 1740
AspS9I GGNCC 7 cut(s) 7, 179, 699, 991, 1208, 1364, 1375
AsuHPI GGTGA 2 cut(s) 380, 1832
AvaI CYCGRG 1 cut(s) 513
AvaII GGWCC 5 cut(s) 7, 179, 699, 991, 1375
AvrII CCTAGG 1 cut(s) 1039
AxyI CCTNAGG 1 cut(s) 1204
BalI TGGCCA 1 cut(s) 399
BanII GRGCYC 2 cut(s) 195, 290
BarI GAAGNNNNNNTAC 2 cut(s) 559, 591
BauI CACGAG 2 cut(s) 415, 837
BbrPI CACGTG 1 cut(s) 1514
BbsI GAAGAC 4 cut(s) 60, 105, 380, 892
Bbv12I GWGCWC 2 cut(s) 290, 838
BbvI GCAGC 5 cut(s) 727, 810, 1093, 1096, 1815
BccI CCATC 4 cut(s) 85, 317, 359, 1526
BceAI ACGGC 2 cut(s) 244, 279
BciT130I CCWGG 3 cut(s) 222, 257, 1223
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 5 cut(s) 269, 447, 516, 1472, 1805
BcuI ACTAGT 3 cut(s) 296, 359, 1699
BfaI CTAG 7 cut(s) 297, 360, 644, 969, 1040, 1425, 1700
BfoI RGCGCY 1 cut(s) 1741
BfuAI ACCTGC 2 cut(s) 1002, 1653
BisI GCNGC 6 cut(s) 267, 741, 799, 1107, 1110, 1829
BlnI CCTAGG 1 cut(s) 1039
BlsI GCNGC 6 cut(s) 268, 742, 800, 1108, 1111, 1830
Bme1390I CCNGG 3 cut(s) 222, 257, 1223
Bme18I GGWCC 5 cut(s) 7, 179, 699, 991, 1375
BmeT110I CYCGRG 1 cut(s) 513
BmgT120I GGNCC 7 cut(s) 7, 179, 699, 991, 1208, 1364, 1375
BmiI GGNNCC 9 cut(s) 9, 180, 181, 254, 700, 992, 1365, 1377, 1540
BmrFI CCNGG 3 cut(s) 222, 257, 1223
BmrI ACTGGG 2 cut(s) 189, 705
BmsI GCATC 4 cut(s) 28, 797, 1322, 1682
BmuI ACTGGG 2 cut(s) 189, 705
BpiI GAAGAC 4 cut(s) 60, 105, 380, 892
BpmI CTGGAG 2 cut(s) 154, 1244
BpuEI CTTGAG 2 cut(s) 581, 602
BsaAI YACGTR 1 cut(s) 1514
BsaBI GATNNNNATC 1 cut(s) 369
BsaHI GRCGYC 2 cut(s) 93, 135
BsaI GGTCTC 2 cut(s) 269, 1805
BsaJI CCNNGG 3 cut(s) 1039, 1221, 1393
BsaWI WCCGGW 2 cut(s) 202, 278
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 262, 708, 1227
Bse118I RCCGGY 1 cut(s) 1102
Bse1I ACTGG 3 cut(s) 195, 439, 700
Bse21I CCTNAGG 1 cut(s) 1204
Bse3DI GCAATG 1 cut(s) 475
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 3 cut(s) 222, 257, 1223
BseDI CCNNGG 3 cut(s) 1039, 1221, 1393
BseGI GGATG 2 cut(s) 13, 77
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 4 cut(s) 227, 262, 708, 1227
BseMI GCAATG 1 cut(s) 475
BseMII CTCAG 4 cut(s) 81, 1075, 1218, 1819
BseNI ACTGG 3 cut(s) 195, 439, 700
BseRI GAGGAG 1 cut(s) 1793
BseXI GCAGC 5 cut(s) 727, 810, 1093, 1096, 1815
BseYI CCCAGC 1 cut(s) 86
BsgI GTGCAG 1 cut(s) 817
Bsh1236I CGCG 3 cut(s) 509, 857, 883
BshFI GGCC 5 cut(s) 327, 399, 438, 1210, 1366
BsiHKAI GWGCWC 2 cut(s) 290, 838
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 3 cut(s) 203, 279, 1103
BslFI GGGAC 5 cut(s) 68, 165, 712, 1004, 1361
BslI CCNNNNNNNGG 4 cut(s) 227, 262, 708, 1227
BsmAI GTCTC 5 cut(s) 269, 447, 516, 1472, 1805
BsmBI CGTCTC 1 cut(s) 516
BsmFI GGGAC 5 cut(s) 68, 165, 712, 1004, 1361
BsmI GAATGC 1 cut(s) 1258
BsnI GGCC 5 cut(s) 327, 399, 438, 1210, 1366
Bso31I GGTCTC 2 cut(s) 269, 1805
BsoBI CYCGRG 1 cut(s) 513
Bsp1286I GDGCHC 3 cut(s) 195, 290, 838
Bsp143I GATC 6 cut(s) 28, 246, 370, 1129, 1559, 1592
BspACI CCGC 6 cut(s) 5, 231, 238, 266, 1311, 1635
BspANI GGCC 5 cut(s) 327, 399, 438, 1210, 1366
BspCNI CTCAG 4 cut(s) 80, 1074, 1217, 1818
BspFNI CGCG 3 cut(s) 509, 857, 883
BspHI TCATGA 2 cut(s) 1765, 1853
BspLI GGNNCC 9 cut(s) 9, 180, 181, 254, 700, 992, 1365, 1377, 1540
BspMI ACCTGC 2 cut(s) 1002, 1653
BspPI GGATC 2 cut(s) 1137, 1600
BspTNI GGTCTC 2 cut(s) 269, 1805
BsrDI GCAATG 1 cut(s) 475
BsrFI RCCGGY 1 cut(s) 1102
BsrI ACTGG 3 cut(s) 195, 439, 700
BssAI RCCGGY 1 cut(s) 1102
BssECI CCNNGG 3 cut(s) 1039, 1221, 1393
BssMI GATC 6 cut(s) 28, 246, 370, 1129, 1559, 1592
BssNAI GTATAC 1 cut(s) 1779
BssNI GRCGYC 2 cut(s) 93, 135
BssSI CACGAG 2 cut(s) 415, 837
BssT1I CCWWGG 2 cut(s) 1039, 1393
Bst1107I GTATAC 1 cut(s) 1779
Bst2BI CACGAG 2 cut(s) 415, 837
Bst2UI CCWGG 3 cut(s) 222, 257, 1223
Bst4CI ACNGT 5 cut(s) 1255, 1303, 1534, 1574, 1732
Bst6I CTCTTC 3 cut(s) 539, 1034, 1841
BstACI GRCGYC 2 cut(s) 93, 135
BstBAI YACGTR 1 cut(s) 1514
BstC8I GCNNGC 3 cut(s) 290, 594, 1036
BstDEI CTNAG 4 cut(s) 67, 1061, 1204, 1805
BstENI CCTNNNNNAGG 1 cut(s) 706
BstF5I GGATG 2 cut(s) 13, 77
BstFNI CGCG 3 cut(s) 509, 857, 883
BstH2I RGCGCY 1 cut(s) 1741
BstHHI GCGC 3 cut(s) 883, 1197, 1740
BstKTI GATC 6 cut(s) 31, 249, 373, 1132, 1562, 1595
BstMAI GTCTC 5 cut(s) 269, 447, 516, 1472, 1805
BstMBI GATC 6 cut(s) 28, 246, 370, 1129, 1559, 1592
BstMWI GCNNNNNNNGC 3 cut(s) 333, 342, 1031
BstNI CCWGG 3 cut(s) 222, 257, 1223
BstNSI RCATGY 4 cut(s) 893, 1122, 1195, 1828
BstSCI CCNGG 3 cut(s) 220, 255, 1221
BstUI CGCG 3 cut(s) 509, 857, 883
BstV1I GCAGC 5 cut(s) 727, 810, 1093, 1096, 1815
BstV2I GAAGAC 4 cut(s) 60, 105, 380, 892
BstX2I RGATCY 2 cut(s) 1129, 1592
BstYI RGATCY 2 cut(s) 1129, 1592
BstZ17I GTATAC 1 cut(s) 1779
Bsu36I CCTNAGG 1 cut(s) 1204
BsuRI GGCC 5 cut(s) 327, 399, 438, 1210, 1366
BtsCI GGATG 2 cut(s) 13, 77
BtsIMutI CAGTG 5 cut(s) 202, 308, 1308, 1605, 1728
BveI ACCTGC 2 cut(s) 1002, 1653
Cac8I GCNNGC 3 cut(s) 290, 594, 1036
CaiI CAGNNNCTG 1 cut(s) 1211
CciI TCATGA 2 cut(s) 1765, 1853
CfoI GCGC 3 cut(s) 883, 1197, 1740
Cfr10I RCCGGY 1 cut(s) 1102
Cfr13I GGNCC 7 cut(s) 7, 179, 699, 991, 1208, 1364, 1375
CseI GACGC 3 cut(s) 498, 846, 863
Csp6I GTAC 2 cut(s) 641, 1842
CviAII CATG 8 cut(s) 890, 1119, 1125, 1192, 1544, 1766, 1825, 1854
CviQI GTAC 2 cut(s) 641, 1842
DdeI CTNAG 4 cut(s) 67, 1061, 1204, 1805
DpnI GATC 6 cut(s) 30, 248, 372, 1131, 1561, 1594
DpnII GATC 6 cut(s) 28, 246, 370, 1129, 1559, 1592
DraI TTTAAA 2 cut(s) 1554, 1707
DraIII CACNNNGTG 1 cut(s) 638
EaeI YGGCCR 1 cut(s) 397
Eam1104I CTCTTC 3 cut(s) 539, 1034, 1841
EarI CTCTTC 3 cut(s) 539, 1034, 1841
EciI GGCGGA 2 cut(s) 20, 246
Ecl136II GAGCTC 1 cut(s) 288
Eco130I CCWWGG 2 cut(s) 1039, 1393
Eco24I GRGCYC 2 cut(s) 195, 290
Eco31I GGTCTC 2 cut(s) 269, 1805
Eco32I GATATC 2 cut(s) 1308, 1618
Eco47I GGWCC 5 cut(s) 7, 179, 699, 991, 1375
Eco53kI GAGCTC 1 cut(s) 288
Eco57I CTGAAG 4 cut(s) 1581, 1604, 1698, 1822
Eco72I CACGTG 1 cut(s) 1514
Eco81I CCTNAGG 1 cut(s) 1204
Eco88I CYCGRG 1 cut(s) 513
EcoICRI GAGCTC 1 cut(s) 288
EcoNI CCTNNNNNAGG 1 cut(s) 706
EcoO109I RGGNCCY 4 cut(s) 179, 699, 991, 1208
EcoRII CCWGG 3 cut(s) 220, 255, 1221
EcoRV GATATC 2 cut(s) 1308, 1618
EcoT14I CCWWGG 2 cut(s) 1039, 1393
EcoT38I GRGCYC 2 cut(s) 195, 290
ErhI CCWWGG 2 cut(s) 1039, 1393
Esp3I CGTCTC 1 cut(s) 516
FaeI CATG 8 cut(s) 893, 1122, 1128, 1195, 1547, 1769, 1828, 1857
FalI AAGNNNNNCTT 2 cut(s) 900, 932
FaqI GGGAC 5 cut(s) 68, 165, 712, 1004, 1361
FatI CATG 8 cut(s) 889, 1118, 1124, 1191, 1543, 1765, 1824, 1853
FauNDI CATATG 1 cut(s) 943
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 1 cut(s) 1778
Fnu4HI GCNGC 6 cut(s) 267, 741, 799, 1107, 1110, 1829
FokI GGATG 1 cut(s) 64
FriOI GRGCYC 2 cut(s) 195, 290
Fsp4HI GCNGC 6 cut(s) 267, 741, 799, 1107, 1110, 1829
FspBI CTAG 7 cut(s) 297, 360, 644, 969, 1040, 1425, 1700
GlaI GCGC 3 cut(s) 882, 1196, 1739
GluI GCNGC 6 cut(s) 267, 741, 799, 1107, 1110, 1829
GsaI CCCAGC 1 cut(s) 90
GsuI CTGGAG 2 cut(s) 154, 1244
HaeII RGCGCY 1 cut(s) 1741
HaeIII GGCC 5 cut(s) 327, 399, 438, 1210, 1366
HapII CCGG 3 cut(s) 203, 279, 1103
HgaI GACGC 3 cut(s) 498, 846, 863
HhaI GCGC 3 cut(s) 883, 1197, 1740
Hin1I GRCGYC 2 cut(s) 93, 135
Hin1II CATG 8 cut(s) 893, 1122, 1128, 1195, 1547, 1769, 1828, 1857
Hin6I GCGC 3 cut(s) 881, 1195, 1738
HinP1I GCGC 3 cut(s) 881, 1195, 1738
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 8 cut(s) 44, 101, 206, 307, 536, 550, 1139, 1217
HpaII CCGG 3 cut(s) 203, 279, 1103
HphI GGTGA 2 cut(s) 380, 1832
Hpy166II GTNNAC 6 cut(s) 48, 200, 557, 641, 782, 1779
Hpy188III TCNNGA 8 cut(s) 32, 171, 250, 513, 581, 723, 1766, 1854
Hpy8I GTNNAC 6 cut(s) 48, 200, 557, 641, 782, 1779
Hpy99I CGWCG 6 cut(s) 29, 38, 62, 95, 98, 857
HpyAV CCTTC 4 cut(s) 580, 604, 808, 1518
HpyCH4III ACNGT 5 cut(s) 1255, 1303, 1534, 1574, 1732
HpyCH4IV ACGT 5 cut(s) 24, 93, 135, 1513, 1608
HpyCH4V TGCA 9 cut(s) 41, 420, 468, 480, 743, 798, 1258, 1673, 1831
HpyF10VI GCNNNNNNNGC 3 cut(s) 333, 342, 1031
HpyF3I CTNAG 4 cut(s) 67, 1061, 1204, 1805
HpySE526I ACGT 5 cut(s) 24, 93, 135, 1513, 1608
Hsp92I GRCGYC 2 cut(s) 93, 135
Hsp92II CATG 8 cut(s) 893, 1122, 1128, 1195, 1547, 1769, 1828, 1857
HspAI GCGC 3 cut(s) 881, 1195, 1738
KflI GGGWCCC 1 cut(s) 179
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 6 cut(s) 28, 246, 370, 1129, 1559, 1592
LmnI GCTCC 3 cut(s) 252, 589, 1711
Lsp1109I GCAGC 5 cut(s) 727, 810, 1093, 1096, 1815
LweI GCATC 4 cut(s) 28, 797, 1322, 1682
MaeI CTAG 7 cut(s) 297, 360, 644, 969, 1040, 1425, 1700
MaeII ACGT 5 cut(s) 24, 93, 135, 1513, 1608
MaeIII GTNAC 3 cut(s) 410, 1354, 1820
MalI GATC 6 cut(s) 30, 248, 372, 1131, 1561, 1594
MboI GATC 6 cut(s) 28, 246, 370, 1129, 1559, 1592
MfeI CAATTG 1 cut(s) 1320
MflI RGATCY 2 cut(s) 1129, 1592
MhlI GDGCHC 3 cut(s) 195, 290, 838
MlsI TGGCCA 1 cut(s) 399
MluI ACGCGT 1 cut(s) 855
MluNI TGGCCA 1 cut(s) 399
MlyI GAGTC 3 cut(s) 53, 95, 215
MmeI TCCRAC 1 cut(s) 189
Mox20I TGGCCA 1 cut(s) 399
MroXI GAANNNNTTC 1 cut(s) 503
MscI TGGCCA 1 cut(s) 399
MseI TTAA 4 cut(s) 875, 1553, 1706, 1743
MslI CAYNNNNRTG 2 cut(s) 793, 1123
Msp20I TGGCCA 1 cut(s) 399
MspI CCGG 3 cut(s) 203, 279, 1103
MspR9I CCNGG 3 cut(s) 222, 257, 1223
MunI CAATTG 1 cut(s) 1320
Mva1269I GAATGC 1 cut(s) 1258
MvaI CCWGG 3 cut(s) 222, 257, 1223
MvnI CGCG 3 cut(s) 509, 857, 883
MwoI GCNNNNNNNGC 3 cut(s) 333, 342, 1031
NdeI CATATG 1 cut(s) 943
NdeII GATC 6 cut(s) 28, 246, 370, 1129, 1559, 1592
NlaIII CATG 8 cut(s) 893, 1122, 1128, 1195, 1547, 1769, 1828, 1857
NlaIV GGNNCC 9 cut(s) 9, 180, 181, 254, 700, 992, 1365, 1377, 1540
NmuCI GTSAC 1 cut(s) 1820
NspI RCATGY 4 cut(s) 893, 1122, 1195, 1828
PaeR7I CTCGAG 1 cut(s) 513
PagI TCATGA 2 cut(s) 1765, 1853
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 33
PctI GAATGC 1 cut(s) 1258
PdmI GAANNNNTTC 1 cut(s) 503
PfeI GAWTC 5 cut(s) 307, 536, 550, 1139, 1217
PkrI GCNGC 6 cut(s) 268, 742, 800, 1108, 1111, 1830
PleI GAGTC 3 cut(s) 52, 95, 214
PmaCI CACGTG 1 cut(s) 1514
PmlI CACGTG 1 cut(s) 1514
PpsI GAGTC 3 cut(s) 52, 95, 214
Ppu21I YACGTR 1 cut(s) 1514
PpuMI RGGWCCY 3 cut(s) 179, 699, 991
Psp124BI GAGCTC 1 cut(s) 290
Psp5II RGGWCCY 3 cut(s) 179, 699, 991
Psp6I CCWGG 3 cut(s) 220, 255, 1221
PspCI CACGTG 1 cut(s) 1514
PspFI CCCAGC 1 cut(s) 86
PspGI CCWGG 3 cut(s) 220, 255, 1221
PspN4I GGNNCC 9 cut(s) 9, 180, 181, 254, 700, 992, 1365, 1377, 1540
PspPI GGNCC 7 cut(s) 7, 179, 699, 991, 1208, 1364, 1375
PspPPI RGGWCCY 3 cut(s) 179, 699, 991
PstNI CAGNNNCTG 1 cut(s) 1211
PsuI RGATCY 2 cut(s) 1129, 1592
RsaI GTAC 2 cut(s) 642, 1843
RsaNI GTAC 2 cut(s) 641, 1842
RseI CAYNNNNRTG 2 cut(s) 793, 1123
SacI GAGCTC 1 cut(s) 290
SaqAI TTAA 4 cut(s) 875, 1553, 1706, 1743
SatI GCNGC 6 cut(s) 267, 741, 799, 1107, 1110, 1829
Sau3AI GATC 6 cut(s) 28, 246, 370, 1129, 1559, 1592
Sau96I GGNCC 7 cut(s) 7, 179, 699, 991, 1208, 1364, 1375
SchI GAGTC 3 cut(s) 53, 95, 215
ScrFI CCNGG 3 cut(s) 222, 257, 1223
SduI GDGCHC 3 cut(s) 195, 290, 838
SfaNI GCATC 4 cut(s) 28, 797, 1322, 1682
Sfr274I CTCGAG 1 cut(s) 513
SinI GGWCC 5 cut(s) 7, 179, 699, 991, 1375
SlaI CTCGAG 1 cut(s) 513
SmiMI CAYNNNNRTG 2 cut(s) 793, 1123
SmlI CTYRAG 3 cut(s) 513, 560, 581
SmoI CTYRAG 3 cut(s) 513, 560, 581
SpeI ACTAGT 3 cut(s) 296, 359, 1699
SsiI CCGC 6 cut(s) 5, 231, 238, 266, 1311, 1635
SspMI CTAG 7 cut(s) 297, 360, 644, 969, 1040, 1425, 1700
SstI GAGCTC 1 cut(s) 290
StyD4I CCNGG 3 cut(s) 220, 255, 1221
StyI CCWWGG 2 cut(s) 1039, 1393
TaaI ACNGT 5 cut(s) 1255, 1303, 1534, 1574, 1732
TaiI ACGT 5 cut(s) 27, 96, 138, 1516, 1611
TaqI TCGA 5 cut(s) 27, 33, 96, 514, 852
TatI WGTACW 1 cut(s) 640
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 5 cut(s) 307, 536, 550, 1139, 1217
Tru1I TTAA 4 cut(s) 875, 1553, 1706, 1743
Tru9I TTAA 4 cut(s) 875, 1553, 1706, 1743
TscAI CASTG 5 cut(s) 202, 308, 1308, 1605, 1735
TseFI GTSAC 1 cut(s) 1820
TseI GCWGC 5 cut(s) 740, 798, 1106, 1109, 1828
Tsp45I GTSAC 1 cut(s) 1820
TspDTI ATGAA 6 cut(s) 330, 546, 1238, 1257, 1754, 1842
TspGWI ACGGA 2 cut(s) 70, 664
TspRI CASTG 5 cut(s) 202, 308, 1308, 1605, 1735
VpaK11BI GGWCC 5 cut(s) 7, 179, 699, 991, 1375
XagI CCTNNNNNAGG 1 cut(s) 706
XapI RAATTY 3 cut(s) 654, 867, 1466
XceI RCATGY 4 cut(s) 893, 1122, 1195, 1828
XhoI CTCGAG 1 cut(s) 513
XmaJI CCTAGG 1 cut(s) 1039
XmiI GTMKAC 1 cut(s) 1778
XmnI GAANNNNTTC 1 cut(s) 503
XspI CTAG 7 cut(s) 297, 360, 644, 969, 1040, 1425, 1700
ZraI GACGTC 2 cut(s) 94, 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.