Rh3CG174800

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0011045
Physical Location & Seq
Reverse (-)
15298457 .. 15303864
5408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG174800.1

Sequence Viewer

Length: 1893 bp
ATGGCAGACCCTCACTATGTGCATCTTGACGATGTAGATCTACAGGAAGAGGATGAAGAAGCCTCAGAAGAACCATCCGTCATCGGCGACGTTGGAGACTCAGAACAAGAAGACGAAGACGAACCAGACGCCCAAACGGATCCCAATTATGCTCTCTATCTTTCCAGAATGGGACCCACTCACGAGGCCGAAGAAGCCGACTCGGATTTTGAACGGCATCTGGTCAGAACCGCTTGCTTTCTGAGCCGCGGCGGCTGCGGCGGTGACGGCAAGCGGAGGAGAATCGGAAGGCAGGAAACGGCGGCGTTTGACACCGGAGAGAGCTCTCAGAGCAGTCAGTGGAATCCGAGTGAAATTGATGGCCTGTTTTGCCCAATTTGCTTGGAGGCTTGGACTAATGACGGTGATCACTACATCTGCTGTCTTCCTTGTGGACACATTTTCGGTATGTCCTGCATCGAAAAATGGCTAGGCCAGAGTAAAAGACAGGGAAAGTGTCCTCAATGCAATGCCAAATGCAAGTTGAATGATGTGAGGAAACTTTTCACGTCGCGAGTTATTTCTGTTGACGAAGAATCACAAAAGAGGATTCGTTCACTTGAGGCTAAATGTGCTTCTTTTGAGAAGGAGCGAGCAGAATGGCATAAGAGGGAGGCAGAATTGCTAAAGGGACAGCATGTACTAGAACTCAAAATTCGACTACTTATAGAGAGCAAAGCATCAGGATCAATCAATGCTACTGGGACCTTTGAAAGAAGATTCGTATCTGGTAATAACATCTTAGAGGGAAGGTCTTATGGGCTAACATTTGGCTCTAATACCTGCGGACAAGCATCTCCTTGCAGCTTCAAATTAGAGAAGGAGTTACCTGTAGCTGGTGCTCATTTGTTTGACGTTGATGCATCTAACCAAATTTTGTTAATTTCGCGAAGGCATGCTGGGCTAGGTGGAAAAGATGTGCTTACCAAAATGAGCCTGATACCTCCATATGACAGAGATGATATTGTTCTTCCTAGCTCTTTAAATTCTATCAGGGACCTACGTCTTTCTCCTTCTAACACTAACCTTGCACTCTTTGCTTGCCTAGGGAAGAAATTGGCAGTTCTCAGTACGGAGAACAACAACATCATTGTTTCCTATGACATACCGGCTGCTGCTTGGACATGCTCATGGGATCTCAACGATTCAAATTATATGTATGCTGGATTGCAGAATGGCTATCTTCTGGTGTTTGACATGCGCCAAACCTCAGGGCCTGTTGAATCCCTGAAGGGGCTGACAAGAAACCCAATTCATACCGTGCATTCCCTTTCATATAATTCAGCTCTTCCCACTGGTGTTAGAACAGTGATGTCCGCATCGTCAATTGGCTTATGTCTCTGGAATTTTGGTGGTGCTGCAAAGGGGCCAATTTTGGTCCCTGAAACAGAGAACCAAGGAGTTTGTATATCTCTTGCATATTGCCATAGAACAGATGACATTGTTGCTTCCTATCGCCCCAAAGTCGAGTTTTCTGATGAGGCAGTTCTTACACAACCAACTCCTTCACGTGCCATCGGACAAGGAACAGTCGGTTCCCATGTTCTTTTAAAGAGAGCAGGCAGCAGCAGTCATTTTCTAAAGTTGGGATCTTCATCCGGCAACGTGTGTGATATCGGACTGCCAAAATCTGCAATTATCAACATAGAAAATTGTGGCAGTTTGTTTGCATCTGAAGATAAACTATCTTCTGAACTAGTTTTAGAAGAGCTGTCGTCTTTTACTACTGTTCAGCGCCTTAACTTACAGCGAAGTCCTGTTCAGGATGTGAAGTACACATCCTTCTTAAGGAAAGGTCTCCTCAGTTGTTTGAATGGTAACACGCTGCAACTCTTCAGTACCAAGCTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

630

Amino Acids

69.07

Weight (kDa)

5.45

Isoelectric Point (pI)

58.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 124 - 170 1.4e-09 Ring finger domain
zf-RING_UBOX PF13445 124 - 167 4.4e-06 RING-type zinc-finger
WD40_RFWD3 PF23419 342 - 625 1.2e-30 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03450 AT5G03750
fragaria_vesca FvH4_6g16560 FvH4_6g16560
malus_domestica MD12G1109200.v1.1
prunus_persica Prupe.7G127800_v2.0.a1
pyrus_communis pycom12g10940
rosa_chinensis RchiOBHm_Chr3g0470481
rosa_laevigata RLG00000024263
rosa_multiflora Rmu_sc0002115.1_g000004 Rmu_sc0003381.1_g000011
rosa_roxburghii Rroxscaffold_4G00302270 Rroxscaffold_6G00410770
rosa_rugosa Rorug03G0109400 Rorug03G0109500
rosa_samantha Rh3AG159800 Rh3BG184300 Rh3CG174800 Rh3DG190600
rosa_wichuraiana Rw3G015120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 830
AccII CGCG 3 cut(s) 249, 553, 928
AclWI GGATC 5 cut(s) 134, 147, 733, 1182, 1636
AcsI RAATTY 4 cut(s) 693, 912, 1024, 1384
AcuI CTGAAG 3 cut(s) 1289, 1734, 1858
AcvI CACGTG 1 cut(s) 1550
AcyI GRCGYC 1 cut(s) 129
AdeI CACNNNGTG 1 cut(s) 19
AfaI GTAC 4 cut(s) 681, 1111, 1814, 1879
AfiI CCNNNNNNNGG 3 cut(s) 875, 1272, 1827
AflII CTTAAG 1 cut(s) 1825
AflIII ACRYGT 1 cut(s) 1644
AgsI TTSAA 7 cut(s) 212, 526, 752, 850, 1188, 1262, 1852
AhlI ACTAGT 1 cut(s) 1735
AjiI CACGTC 1 cut(s) 549
AluBI AGCT 7 cut(s) 324, 846, 875, 1017, 1325, 1750, 1885
AluI AGCT 7 cut(s) 324, 846, 875, 1017, 1325, 1750, 1885
Alw21I GWGCWC 2 cut(s) 326, 883
Alw26I GTCTC 3 cut(s) 90, 1382, 1841
AlwI GGATC 5 cut(s) 134, 147, 733, 1182, 1636
AlwNI CAGNNNCTG 1 cut(s) 1256
AoxI GGCC 5 cut(s) 186, 361, 472, 1253, 1406
ApeKI GCWGC 8 cut(s) 255, 843, 1151, 1154, 1397, 1602, 1605, 1864
ApoI RAATTY 4 cut(s) 693, 912, 1024, 1384
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 1 cut(s) 542
AspA2I CCTAGG 1 cut(s) 1084
AspLEI GCGC 2 cut(s) 1242, 1776
AspS9I GGNCC 6 cut(s) 173, 744, 1036, 1253, 1406, 1417
AsuHPI GGTGA 2 cut(s) 275, 416
AvaII GGWCC 4 cut(s) 173, 744, 1036, 1417
AvrII CCTAGG 1 cut(s) 1084
AxyI CCTNAGG 1 cut(s) 1249
BamHI GGATCC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 326
BarI GAAGNNNNNNTAC 2 cut(s) 1805, 1837
BauI CACGAG 1 cut(s) 182
BbrPI CACGTG 1 cut(s) 1550
BbsI GAAGAC 3 cut(s) 117, 123, 416
Bbv12I GWGCWC 2 cut(s) 326, 883
BbvI GCAGC 8 cut(s) 242, 855, 1138, 1141, 1384, 1614, 1617, 1851
BccI CCATC 3 cut(s) 82, 353, 1562
BceAI ACGGC 3 cut(s) 230, 283, 315
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 3 cut(s) 90, 1382, 1841
BcuI ACTAGT 1 cut(s) 1735
BfaI CTAG 6 cut(s) 470, 683, 944, 1014, 1085, 1736
BfmI CTRYAG 2 cut(s) 41, 870
BfoI RGCGCY 1 cut(s) 1777
BfrI CTTAAG 1 cut(s) 1825
BfuAI ACCTGC 1 cut(s) 830
BglI GCCNNNNNGGC 1 cut(s) 252
BglII AGATCT 1 cut(s) 37
BlnI CCTAGG 1 cut(s) 1084
Bme18I GGWCC 4 cut(s) 173, 744, 1036, 1417
BmgBI CACGTC 1 cut(s) 549
BmgT120I GGNCC 6 cut(s) 173, 744, 1036, 1253, 1406, 1417
BmiI GGNNCC 8 cut(s) 141, 174, 175, 745, 1037, 1407, 1419, 1576
BmrI ACTGGG 1 cut(s) 750
BmsI GCATC 9 cut(s) 31, 226, 465, 728, 842, 889, 911, 1367, 1718
BmuI ACTGGG 1 cut(s) 750
BoxI GACNNNNGTC 1 cut(s) 1041
BpiI GAAGAC 3 cut(s) 117, 123, 416
BpuEI CTTGAG 1 cut(s) 620
BsaAI YACGTR 1 cut(s) 1550
BsaBI GATNNNNATC 3 cut(s) 36, 763, 1633
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 1841
BsaJI CCNNGG 3 cut(s) 247, 1084, 1435
BsaWI WCCGGW 1 cut(s) 314
Bsc4I CCNNNNNNNGG 3 cut(s) 875, 1272, 1827
Bse118I RCCGGY 1 cut(s) 1147
Bse1I ACTGG 2 cut(s) 745, 1339
Bse21I CCTNAGG 1 cut(s) 1249
Bse3DI GCAATG 1 cut(s) 514
Bse8I GATNNNNATC 3 cut(s) 36, 763, 1633
BseDI CCNNGG 3 cut(s) 247, 1084, 1435
BseGI GGATG 5 cut(s) 58, 74, 1634, 1810, 1817
BseJI GATNNNNATC 3 cut(s) 36, 763, 1633
BseLI CCNNNNNNNGG 3 cut(s) 875, 1272, 1827
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 7 cut(s) 78, 114, 233, 341, 1120, 1263, 1855
BseNI ACTGG 2 cut(s) 745, 1339
BseRI GAGGAG 2 cut(s) 292, 1829
BseXI GCAGC 8 cut(s) 242, 855, 1138, 1141, 1384, 1614, 1617, 1851
BseYI CCCAGC 1 cut(s) 938
Bsh1236I CGCG 3 cut(s) 249, 553, 928
BshFI GGCC 5 cut(s) 188, 363, 474, 1255, 1408
BsiHKAI GWGCWC 2 cut(s) 326, 883
BsiSI CCGG 3 cut(s) 315, 1148, 1638
BslFI GGGAC 5 cut(s) 186, 684, 757, 1049, 1403
BslI CCNNNNNNNGG 3 cut(s) 875, 1272, 1827
BsmAI GTCTC 3 cut(s) 90, 1382, 1841
BsmFI GGGAC 5 cut(s) 186, 684, 757, 1049, 1403
BsmI GAATGC 1 cut(s) 1303
BsnI GGCC 5 cut(s) 188, 363, 474, 1255, 1408
Bso31I GGTCTC 1 cut(s) 1841
Bsp1286I GDGCHC 2 cut(s) 326, 883
Bsp143I GATC 6 cut(s) 37, 139, 406, 725, 1174, 1628
Bsp68I TCGCGA 2 cut(s) 553, 928
BspANI GGCC 5 cut(s) 188, 363, 474, 1255, 1408
BspCNI CTCAG 7 cut(s) 77, 113, 234, 340, 1119, 1262, 1854
BspFNI CGCG 3 cut(s) 249, 553, 928
BspHI TCATGA 1 cut(s) 1889
BspLI GGNNCC 8 cut(s) 141, 174, 175, 745, 1037, 1407, 1419, 1576
BspMI ACCTGC 1 cut(s) 830
BspPI GGATC 5 cut(s) 134, 147, 733, 1182, 1636
BspQI GCTCTTC 2 cut(s) 1332, 1740
BspTI CTTAAG 1 cut(s) 1825
BspTNI GGTCTC 1 cut(s) 1841
BsrDI GCAATG 1 cut(s) 514
BsrFI RCCGGY 1 cut(s) 1147
BsrI ACTGG 2 cut(s) 745, 1339
BssAI RCCGGY 1 cut(s) 1147
BssECI CCNNGG 3 cut(s) 247, 1084, 1435
BssMI GATC 6 cut(s) 37, 139, 406, 725, 1174, 1628
BssNI GRCGYC 1 cut(s) 129
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 2 cut(s) 1084, 1435
Bst2BI CACGAG 1 cut(s) 182
Bst4CI ACNGT 5 cut(s) 404, 1300, 1348, 1570, 1768
Bst6I CTCTTC 4 cut(s) 42, 1332, 1740, 1877
BstACI GRCGYC 1 cut(s) 129
BstAFI CTTAAG 1 cut(s) 1825
BstAPI GCANNNNNTGC 1 cut(s) 1076
BstBAI YACGTR 1 cut(s) 1550
BstC8I GCNNGC 6 cut(s) 235, 272, 633, 936, 1081, 1600
BstDEI CTNAG 8 cut(s) 64, 100, 242, 327, 781, 1106, 1249, 1841
BstDSI CCRYGG 1 cut(s) 247
BstENI CCTNNNNNAGG 1 cut(s) 1825
BstF5I GGATG 5 cut(s) 58, 74, 1634, 1810, 1817
BstFNI CGCG 3 cut(s) 249, 553, 928
BstH2I RGCGCY 1 cut(s) 1777
BstHHI GCGC 2 cut(s) 1242, 1776
BstKTI GATC 6 cut(s) 40, 142, 409, 728, 1177, 1631
BstMAI GTCTC 3 cut(s) 90, 1382, 1841
BstMBI GATC 6 cut(s) 37, 139, 406, 725, 1174, 1628
BstNSI RCATGY 4 cut(s) 680, 938, 1167, 1240
BstPAI GACNNNNGTC 1 cut(s) 1041
BstSFI CTRYAG 2 cut(s) 41, 870
BstUI CGCG 3 cut(s) 249, 553, 928
BstV1I GCAGC 8 cut(s) 242, 855, 1138, 1141, 1384, 1614, 1617, 1851
BstV2I GAAGAC 3 cut(s) 117, 123, 416
BstX2I RGATCY 4 cut(s) 37, 139, 1174, 1628
BstYI RGATCY 4 cut(s) 37, 139, 1174, 1628
Bsu36I CCTNAGG 1 cut(s) 1249
BsuRI GGCC 5 cut(s) 188, 363, 474, 1255, 1408
BtgI CCRYGG 1 cut(s) 247
BtrI CACGTC 1 cut(s) 549
BtsCI GGATG 5 cut(s) 58, 74, 1634, 1810, 1817
BtsIMutI CAGTG 3 cut(s) 344, 1332, 1353
BtuMI TCGCGA 2 cut(s) 553, 928
BveI ACCTGC 1 cut(s) 830
Cac8I GCNNGC 6 cut(s) 235, 272, 633, 936, 1081, 1600
CaiI CAGNNNCTG 1 cut(s) 1256
CciI TCATGA 1 cut(s) 1889
CfoI GCGC 2 cut(s) 1242, 1776
Cfr10I RCCGGY 1 cut(s) 1147
Cfr13I GGNCC 6 cut(s) 173, 744, 1036, 1253, 1406, 1417
Cfr42I CCGCGG 1 cut(s) 250
CseI GACGC 1 cut(s) 137
Csp6I GTAC 4 cut(s) 680, 1110, 1813, 1878
CviAII CATG 7 cut(s) 677, 935, 1164, 1170, 1237, 1580, 1890
CviQI GTAC 4 cut(s) 680, 1110, 1813, 1878
DdeI CTNAG 8 cut(s) 64, 100, 242, 327, 781, 1106, 1249, 1841
DpnI GATC 6 cut(s) 39, 141, 408, 727, 1176, 1630
DpnII GATC 6 cut(s) 37, 139, 406, 725, 1174, 1628
DraI TTTAAA 2 cut(s) 1023, 1590
DraIII CACNNNGTG 1 cut(s) 19
Eam1104I CTCTTC 4 cut(s) 42, 1332, 1740, 1877
EarI CTCTTC 4 cut(s) 42, 1332, 1740, 1877
Ecl136II GAGCTC 1 cut(s) 324
Eco130I CCWWGG 2 cut(s) 1084, 1435
Eco24I GRGCYC 1 cut(s) 326
Eco31I GGTCTC 1 cut(s) 1841
Eco32I GATATC 1 cut(s) 1654
Eco47I GGWCC 4 cut(s) 173, 744, 1036, 1417
Eco53kI GAGCTC 1 cut(s) 324
Eco57I CTGAAG 3 cut(s) 1289, 1734, 1858
Eco72I CACGTG 1 cut(s) 1550
Eco81I CCTNAGG 1 cut(s) 1249
EcoICRI GAGCTC 1 cut(s) 324
EcoNI CCTNNNNNAGG 1 cut(s) 1825
EcoO109I RGGNCCY 4 cut(s) 173, 744, 1036, 1253
EcoRV GATATC 1 cut(s) 1654
EcoT14I CCWWGG 2 cut(s) 1084, 1435
EcoT22I ATGCAT 1 cut(s) 904
EcoT38I GRGCYC 1 cut(s) 326
ErhI CCWWGG 2 cut(s) 1084, 1435
FaeI CATG 7 cut(s) 680, 938, 1167, 1173, 1240, 1583, 1893
FalI AAGNNNNNCTT 2 cut(s) 945, 977
FaqI GGGAC 5 cut(s) 186, 684, 757, 1049, 1403
FatI CATG 7 cut(s) 676, 934, 1163, 1169, 1236, 1579, 1889
FauNDI CATATG 1 cut(s) 988
FbaI TGATCA 1 cut(s) 406
FokI GGATG 5 cut(s) 61, 65, 1621, 1804, 1817
FriOI GRGCYC 1 cut(s) 326
FspBI CTAG 6 cut(s) 470, 683, 944, 1014, 1085, 1736
GlaI GCGC 2 cut(s) 1241, 1775
GsaI CCCAGC 1 cut(s) 942
HaeII RGCGCY 1 cut(s) 1777
HaeIII GGCC 5 cut(s) 188, 363, 474, 1255, 1408
HapII CCGG 3 cut(s) 315, 1148, 1638
HgaI GACGC 1 cut(s) 137
HhaI GCGC 2 cut(s) 1242, 1776
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 7 cut(s) 680, 938, 1167, 1173, 1240, 1583, 1893
Hin6I GCGC 2 cut(s) 1240, 1774
HinP1I GCGC 2 cut(s) 1240, 1774
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 1 cut(s) 1883
HinfI GANTC 9 cut(s) 98, 200, 282, 343, 575, 589, 759, 1184, 1262
HpaII CCGG 3 cut(s) 315, 1148, 1638
HphI GGTGA 2 cut(s) 275, 416
Hpy166II GTNNAC 4 cut(s) 434, 568, 596, 1815
Hpy188III TCNNGA 9 cut(s) 26, 165, 182, 552, 723, 927, 1381, 1802, 1890
Hpy8I GTNNAC 4 cut(s) 434, 568, 596, 1815
Hpy99I CGWCG 2 cut(s) 92, 553
HpyAV CCTTC 9 cut(s) 282, 619, 783, 853, 924, 1062, 1264, 1554, 1831
HpyCH4III ACNGT 5 cut(s) 404, 1300, 1348, 1570, 1768
HpyCH4IV ACGT 6 cut(s) 90, 548, 894, 1042, 1549, 1644
HpyF3I CTNAG 8 cut(s) 64, 100, 242, 327, 781, 1106, 1249, 1841
HpySE526I ACGT 6 cut(s) 90, 548, 894, 1042, 1549, 1644
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 7 cut(s) 680, 938, 1167, 1173, 1240, 1583, 1893
HspAI GCGC 2 cut(s) 1240, 1774
KflI GGGWCCC 1 cut(s) 173
Ksp22I TGATCA 1 cut(s) 406
KspI CCGCGG 1 cut(s) 250
Kzo9I GATC 6 cut(s) 37, 139, 406, 725, 1174, 1628
LguI GCTCTTC 2 cut(s) 1332, 1740
LmnI GCTCC 1 cut(s) 628
Lsp1109I GCAGC 8 cut(s) 242, 855, 1138, 1141, 1384, 1614, 1617, 1851
LweI GCATC 9 cut(s) 31, 226, 465, 728, 842, 889, 911, 1367, 1718
MaeI CTAG 6 cut(s) 470, 683, 944, 1014, 1085, 1736
MaeII ACGT 6 cut(s) 90, 548, 894, 1042, 1549, 1644
MaeIII GTNAC 3 cut(s) 263, 864, 1856
MalI GATC 6 cut(s) 39, 141, 408, 727, 1176, 1630
MboI GATC 6 cut(s) 37, 139, 406, 725, 1174, 1628
MfeI CAATTG 1 cut(s) 1365
MflI RGATCY 4 cut(s) 37, 139, 1174, 1628
MhlI GDGCHC 2 cut(s) 326, 883
MlyI GAGTC 2 cut(s) 92, 194
MmeI TCCRAC 1 cut(s) 73
Mph1103I ATGCAT 1 cut(s) 904
MroXI GAANNNNTTC 1 cut(s) 542
MseI TTAA 5 cut(s) 920, 1022, 1589, 1779, 1826
MslI CAYNNNNRTG 1 cut(s) 1168
MspA1I CMGCKG 1 cut(s) 249
MspCI CTTAAG 1 cut(s) 1825
MspI CCGG 3 cut(s) 315, 1148, 1638
MunI CAATTG 1 cut(s) 1365
Mva1269I GAATGC 1 cut(s) 1303
MvnI CGCG 3 cut(s) 249, 553, 928
NdeI CATATG 1 cut(s) 988
NdeII GATC 6 cut(s) 37, 139, 406, 725, 1174, 1628
NlaIII CATG 7 cut(s) 680, 938, 1167, 1173, 1240, 1583, 1893
NlaIV GGNNCC 8 cut(s) 141, 174, 175, 745, 1037, 1407, 1419, 1576
NmuCI GTSAC 1 cut(s) 263
NruI TCGCGA 2 cut(s) 553, 928
NsiI ATGCAT 1 cut(s) 904
NspI RCATGY 4 cut(s) 680, 938, 1167, 1240
PaeI GCATGC 1 cut(s) 938
PagI TCATGA 1 cut(s) 1889
PciSI GCTCTTC 2 cut(s) 1332, 1740
PctI GAATGC 1 cut(s) 1303
PdmI GAANNNNTTC 1 cut(s) 542
PfeI GAWTC 7 cut(s) 282, 343, 575, 589, 759, 1184, 1262
PleI GAGTC 2 cut(s) 92, 194
PmaCI CACGTG 1 cut(s) 1550
PmlI CACGTG 1 cut(s) 1550
PpsI GAGTC 2 cut(s) 92, 194
Ppu21I YACGTR 1 cut(s) 1550
PpuMI RGGWCCY 3 cut(s) 173, 744, 1036
PshAI GACNNNNGTC 1 cut(s) 1041
Psp124BI GAGCTC 1 cut(s) 326
Psp5II RGGWCCY 3 cut(s) 173, 744, 1036
PspCI CACGTG 1 cut(s) 1550
PspFI CCCAGC 1 cut(s) 938
PspN4I GGNNCC 8 cut(s) 141, 174, 175, 745, 1037, 1407, 1419, 1576
PspPI GGNCC 6 cut(s) 173, 744, 1036, 1253, 1406, 1417
PspPPI RGGWCCY 3 cut(s) 173, 744, 1036
PstNI CAGNNNCTG 1 cut(s) 1256
PsuI RGATCY 4 cut(s) 37, 139, 1174, 1628
RruI TCGCGA 2 cut(s) 553, 928
RsaI GTAC 4 cut(s) 681, 1111, 1814, 1879
RsaNI GTAC 4 cut(s) 680, 1110, 1813, 1878
RseI CAYNNNNRTG 1 cut(s) 1168
SacI GAGCTC 1 cut(s) 326
SacII CCGCGG 1 cut(s) 250
SapI GCTCTTC 2 cut(s) 1332, 1740
SaqAI TTAA 5 cut(s) 920, 1022, 1589, 1779, 1826
Sau3AI GATC 6 cut(s) 37, 139, 406, 725, 1174, 1628
Sau96I GGNCC 6 cut(s) 173, 744, 1036, 1253, 1406, 1417
SchI GAGTC 2 cut(s) 92, 194
SduI GDGCHC 2 cut(s) 326, 883
SfaNI GCATC 9 cut(s) 31, 226, 465, 728, 842, 889, 911, 1367, 1718
SfcI CTRYAG 2 cut(s) 41, 870
Sfr303I CCGCGG 1 cut(s) 250
SgrBI CCGCGG 1 cut(s) 250
SinI GGWCC 4 cut(s) 173, 744, 1036, 1417
SmiMI CAYNNNNRTG 1 cut(s) 1168
SmlI CTYRAG 2 cut(s) 599, 1825
SmoI CTYRAG 2 cut(s) 599, 1825
SpeI ACTAGT 1 cut(s) 1735
SphI GCATGC 1 cut(s) 938
SspMI CTAG 6 cut(s) 470, 683, 944, 1014, 1085, 1736
SstI GAGCTC 1 cut(s) 326
StyI CCWWGG 2 cut(s) 1084, 1435
TaaI ACNGT 5 cut(s) 404, 1300, 1348, 1570, 1768
TaiI ACGT 6 cut(s) 93, 551, 897, 1045, 1552, 1647
TaqI TCGA 3 cut(s) 459, 697, 1506
TatI WGTACW 2 cut(s) 679, 1812
TauI GCSGC 5 cut(s) 249, 252, 255, 261, 305
TfiI GAWTC 7 cut(s) 282, 343, 575, 589, 759, 1184, 1262
Tru1I TTAA 5 cut(s) 920, 1022, 1589, 1779, 1826
Tru9I TTAA 5 cut(s) 920, 1022, 1589, 1779, 1826
TscAI CASTG 3 cut(s) 344, 1339, 1353
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 8 cut(s) 255, 843, 1151, 1154, 1397, 1602, 1605, 1864
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 5 cut(s) 69, 1283, 1302, 1623, 1878
TspGWI ACGGA 3 cut(s) 67, 152, 1127
TspRI CASTG 3 cut(s) 344, 1339, 1353
Vha464I CTTAAG 1 cut(s) 1825
VpaK11BI GGWCC 4 cut(s) 173, 744, 1036, 1417
XagI CCTNNNNNAGG 1 cut(s) 1825
XapI RAATTY 4 cut(s) 693, 912, 1024, 1384
XceI RCATGY 4 cut(s) 680, 938, 1167, 1240
XmaJI CCTAGG 1 cut(s) 1084
XmnI GAANNNNTTC 1 cut(s) 542
XspI CTAG 6 cut(s) 470, 683, 944, 1014, 1085, 1736
Zsp2I ATGCAT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.