RchiOBHm_Chr3g0470481

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0011045
Physical Location & Seq
Reverse (-)
16444997 .. 16450284
5288 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43627

Sequence Viewer

Length: 1863 bp
ATGGCAGACCCTCACTATGTGCATCTTGACGATGTAGATCTACAGGAAGAGGATGAAGAAGCCTCAGAAGAACCATCCGTCATCGGCGACGTTGGAGACTCAGAACAAGAAGACGAAGACGAACCAGACGCCCAAACGGATCCCAATTATGCTCTCTATCTTTCCAGAATGGGACCCACTCACGAGGCCGAAGAAGCCGACTCGGATTTTGAACGGCATCTGGTCAGAACCGCTTGCTTTCTGAGCCGCGGCGGCTGCGGCGGTGACGGCAAGCGGAGGAGAATCGGAAGGCAGGAAACGGCGGCGTTTGACACCGGAGAGAGCTCTCAGAGCAGTCAGTGGAATCCGAGTGAAATTGATGGCCTGTTTTGCCCAATTTGCTTGGAGGCTTGGACTAATGACGGTGATCACTACATCTGCTGTCTTCCTTGTGGACACATTTTCGGTATGTCCTGCATCGAAAAATGGCTAGGCCAGAGTAAAAGACAGGGAAAGTGTCCTCAATGCAATGCCAAATGCAAGTTGAATGATGTGAGGAAACTTTTCACGTCGCGAGTTATTTCTGTTGACGAAGAATCACAAAAGAGGATTCGTTCACTTGAGGCTAAATGTGCTTCTTTTGAGAAGGAGCGAGCAGAATGGCATAAGAGGGAGGCAGAATTGCTAAAGGGACAGCATGTACTAGAACTCAAAATTCGACTACTTATAGAGAGCAAAGCATCAGGATCAATCAATGCTACTGGGACCTTTGAAAGAAGATTCGTATCTGGGCTAACATTTGGCTCTAATACCTGCGGACAAGCATCTCCTTGCAGCTTCAAATTAGAGAAGGAGTTACCTGTAGCTGGTGCTCATTTGTTTGACGTTGATGCATCTAACCAAATTTTGTTAATTTCGCGAAGGCATGCTGGGCTAGGTGGAAAAGATGTGCTTACCAAAATGAGCCTGATACCTCCATATGACAGAGATGATATTGTTCTTCCTAGCTCTTTAAATTCTATCAGGGACCTACGTCTTTCTCCTTCTAACACTAACCTTGCACTCTTTGCTTGCCTAGGGAAGAAATTGGCAGTTCTCAGTACGGAGAACAACAACATCATTGTTTCCTATGACATACCGGCTGCTGCTTGGACATGCTCATGGGATCTCAACGATTCAAATTATATGTATGCTGGATTGCAGAATGGCTATCTTCTGGTGTTTGACATGCGCCAAACCTCAGGGCCTGTTGAATCCCTGAAGGGGCTGACAAGAAACCCAATTCATACCGTGCATTCCCTTTCATATAATTCAGCTCTTCCCACTGGTGTTAGAACAGTGATGTCCGCATCGTCAATTGGCTTATGTCTCTGGAATTTTGGTGGTGCTGCAAAGGGGCCAATTTTGGTCCCTGAAACAGAGAACCAAGGAGTTTGTATATCTCTTGCATATTGCCATAGAACAGATGACATTGTTGCTTCCTATCGCCCCAAAGTCGAGTTTTCTGATGAGGCAGTTCTTACACAACCAACTCCTTCACGTGCCATCGGACAAGGAACAGTCGGTTCCCATGTTCTTTTAAAGAGAGCAGGCAGCAGCAGTCATTTTCTAAAGTTGGGATCTTCATCCGGCAACGTGTGTGATATCGGACTGCCAAAATCTGCAATTATCAACATAGAAAATTGTGGCAGTTTGTTTGCATCTGAAGATAAACTATCTTCTGAACTAGTTTTAGAAGAGCTGTCGTCTTTTACTACTGTTCAGCGCCTTAACTTACAGCGAAGTCCTGTTCAGGATGTGAAGTACACATCCTTCTTAAGGAAAGGTCTCCTCAGTTGTTTGAATGGTAACACGCTGCAACTCTTCAGTACCAAGCTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

620

Amino Acids

67.96

Weight (kDa)

5.44

Isoelectric Point (pI)

58.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 124 - 170 1.4e-09 Ring finger domain
zf-RING_UBOX PF13445 124 - 167 4.4e-06 RING-type zinc-finger
WD40_RFWD3 PF23419 332 - 615 1.2e-30 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03450 AT5G03750
fragaria_vesca FvH4_6g16560 FvH4_6g16560
malus_domestica MD12G1109200.v1.1
prunus_persica Prupe.7G127800_v2.0.a1
pyrus_communis pycom12g10940
rosa_chinensis RchiOBHm_Chr3g0470481
rosa_laevigata RLG00000024263
rosa_multiflora Rmu_sc0002115.1_g000004 Rmu_sc0003381.1_g000011
rosa_roxburghii Rroxscaffold_4G00302270 Rroxscaffold_6G00410770
rosa_rugosa Rorug03G0109400 Rorug03G0109500
rosa_samantha Rh3AG159800 Rh3BG184300 Rh3CG174800 Rh3DG190600
rosa_wichuraiana Rw3G015120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 800
AccII CGCG 3 cut(s) 249, 553, 898
AclWI GGATC 5 cut(s) 134, 147, 733, 1152, 1606
AcsI RAATTY 4 cut(s) 693, 882, 994, 1354
AcuI CTGAAG 3 cut(s) 1259, 1704, 1828
AcvI CACGTG 1 cut(s) 1520
AcyI GRCGYC 1 cut(s) 129
AdeI CACNNNGTG 1 cut(s) 19
AfaI GTAC 4 cut(s) 681, 1081, 1784, 1849
AfiI CCNNNNNNNGG 3 cut(s) 845, 1242, 1797
AflII CTTAAG 1 cut(s) 1795
AflIII ACRYGT 1 cut(s) 1614
AgsI TTSAA 7 cut(s) 212, 526, 752, 820, 1158, 1232, 1822
AhlI ACTAGT 1 cut(s) 1705
AjiI CACGTC 1 cut(s) 549
AluBI AGCT 7 cut(s) 324, 816, 845, 987, 1295, 1720, 1855
AluI AGCT 7 cut(s) 324, 816, 845, 987, 1295, 1720, 1855
Alw21I GWGCWC 2 cut(s) 326, 853
Alw26I GTCTC 3 cut(s) 90, 1352, 1811
AlwI GGATC 5 cut(s) 134, 147, 733, 1152, 1606
AlwNI CAGNNNCTG 1 cut(s) 1226
AoxI GGCC 5 cut(s) 186, 361, 472, 1223, 1376
ApeKI GCWGC 8 cut(s) 255, 813, 1121, 1124, 1367, 1572, 1575, 1834
ApoI RAATTY 4 cut(s) 693, 882, 994, 1354
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 1 cut(s) 542
AspA2I CCTAGG 1 cut(s) 1054
AspLEI GCGC 2 cut(s) 1212, 1746
AspS9I GGNCC 6 cut(s) 173, 744, 1006, 1223, 1376, 1387
AsuHPI GGTGA 2 cut(s) 275, 416
AvaII GGWCC 4 cut(s) 173, 744, 1006, 1387
AvrII CCTAGG 1 cut(s) 1054
AxyI CCTNAGG 1 cut(s) 1219
BamHI GGATCC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 326
BarI GAAGNNNNNNTAC 2 cut(s) 1775, 1807
BauI CACGAG 1 cut(s) 182
BbrPI CACGTG 1 cut(s) 1520
BbsI GAAGAC 3 cut(s) 117, 123, 416
Bbv12I GWGCWC 2 cut(s) 326, 853
BbvI GCAGC 8 cut(s) 242, 825, 1108, 1111, 1354, 1584, 1587, 1821
BccI CCATC 3 cut(s) 82, 353, 1532
BceAI ACGGC 3 cut(s) 230, 283, 315
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 3 cut(s) 90, 1352, 1811
BcuI ACTAGT 1 cut(s) 1705
BfaI CTAG 6 cut(s) 470, 683, 914, 984, 1055, 1706
BfmI CTRYAG 2 cut(s) 41, 840
BfoI RGCGCY 1 cut(s) 1747
BfrI CTTAAG 1 cut(s) 1795
BfuAI ACCTGC 1 cut(s) 800
BglI GCCNNNNNGGC 1 cut(s) 252
BglII AGATCT 1 cut(s) 37
BlnI CCTAGG 1 cut(s) 1054
Bme18I GGWCC 4 cut(s) 173, 744, 1006, 1387
BmgBI CACGTC 1 cut(s) 549
BmgT120I GGNCC 6 cut(s) 173, 744, 1006, 1223, 1376, 1387
BmiI GGNNCC 8 cut(s) 141, 174, 175, 745, 1007, 1377, 1389, 1546
BmrI ACTGGG 1 cut(s) 750
BmsI GCATC 9 cut(s) 31, 226, 465, 728, 812, 859, 881, 1337, 1688
BmuI ACTGGG 1 cut(s) 750
BoxI GACNNNNGTC 1 cut(s) 1011
BpiI GAAGAC 3 cut(s) 117, 123, 416
BpuEI CTTGAG 1 cut(s) 620
BsaAI YACGTR 1 cut(s) 1520
BsaBI GATNNNNATC 3 cut(s) 36, 763, 1603
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 1811
BsaJI CCNNGG 3 cut(s) 247, 1054, 1405
BsaWI WCCGGW 1 cut(s) 314
Bsc4I CCNNNNNNNGG 3 cut(s) 845, 1242, 1797
Bse118I RCCGGY 1 cut(s) 1117
Bse1I ACTGG 2 cut(s) 745, 1309
Bse21I CCTNAGG 1 cut(s) 1219
Bse3DI GCAATG 1 cut(s) 514
Bse8I GATNNNNATC 3 cut(s) 36, 763, 1603
BseDI CCNNGG 3 cut(s) 247, 1054, 1405
BseGI GGATG 5 cut(s) 58, 74, 1604, 1780, 1787
BseJI GATNNNNATC 3 cut(s) 36, 763, 1603
BseLI CCNNNNNNNGG 3 cut(s) 845, 1242, 1797
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 7 cut(s) 78, 114, 233, 341, 1090, 1233, 1825
BseNI ACTGG 2 cut(s) 745, 1309
BseRI GAGGAG 2 cut(s) 292, 1799
BseXI GCAGC 8 cut(s) 242, 825, 1108, 1111, 1354, 1584, 1587, 1821
BseYI CCCAGC 1 cut(s) 908
Bsh1236I CGCG 3 cut(s) 249, 553, 898
BshFI GGCC 5 cut(s) 188, 363, 474, 1225, 1378
BsiHKAI GWGCWC 2 cut(s) 326, 853
BsiSI CCGG 3 cut(s) 315, 1118, 1608
BslFI GGGAC 5 cut(s) 186, 684, 757, 1019, 1373
BslI CCNNNNNNNGG 3 cut(s) 845, 1242, 1797
BsmAI GTCTC 3 cut(s) 90, 1352, 1811
BsmFI GGGAC 5 cut(s) 186, 684, 757, 1019, 1373
BsmI GAATGC 1 cut(s) 1273
BsnI GGCC 5 cut(s) 188, 363, 474, 1225, 1378
Bso31I GGTCTC 1 cut(s) 1811
Bsp1286I GDGCHC 2 cut(s) 326, 853
Bsp143I GATC 6 cut(s) 37, 139, 406, 725, 1144, 1598
Bsp68I TCGCGA 2 cut(s) 553, 898
BspANI GGCC 5 cut(s) 188, 363, 474, 1225, 1378
BspCNI CTCAG 7 cut(s) 77, 113, 234, 340, 1089, 1232, 1824
BspFNI CGCG 3 cut(s) 249, 553, 898
BspHI TCATGA 1 cut(s) 1859
BspLI GGNNCC 8 cut(s) 141, 174, 175, 745, 1007, 1377, 1389, 1546
BspMI ACCTGC 1 cut(s) 800
BspPI GGATC 5 cut(s) 134, 147, 733, 1152, 1606
BspQI GCTCTTC 2 cut(s) 1302, 1710
BspTI CTTAAG 1 cut(s) 1795
BspTNI GGTCTC 1 cut(s) 1811
BsrDI GCAATG 1 cut(s) 514
BsrFI RCCGGY 1 cut(s) 1117
BsrI ACTGG 2 cut(s) 745, 1309
BssAI RCCGGY 1 cut(s) 1117
BssECI CCNNGG 3 cut(s) 247, 1054, 1405
BssMI GATC 6 cut(s) 37, 139, 406, 725, 1144, 1598
BssNI GRCGYC 1 cut(s) 129
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 2 cut(s) 1054, 1405
Bst2BI CACGAG 1 cut(s) 182
Bst4CI ACNGT 5 cut(s) 404, 1270, 1318, 1540, 1738
Bst6I CTCTTC 4 cut(s) 42, 1302, 1710, 1847
BstACI GRCGYC 1 cut(s) 129
BstAFI CTTAAG 1 cut(s) 1795
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstBAI YACGTR 1 cut(s) 1520
BstC8I GCNNGC 6 cut(s) 235, 272, 633, 906, 1051, 1570
BstDEI CTNAG 7 cut(s) 64, 100, 242, 327, 1076, 1219, 1811
BstDSI CCRYGG 1 cut(s) 247
BstENI CCTNNNNNAGG 1 cut(s) 1795
BstF5I GGATG 5 cut(s) 58, 74, 1604, 1780, 1787
BstFNI CGCG 3 cut(s) 249, 553, 898
BstH2I RGCGCY 1 cut(s) 1747
BstHHI GCGC 2 cut(s) 1212, 1746
BstKTI GATC 6 cut(s) 40, 142, 409, 728, 1147, 1601
BstMAI GTCTC 3 cut(s) 90, 1352, 1811
BstMBI GATC 6 cut(s) 37, 139, 406, 725, 1144, 1598
BstNSI RCATGY 4 cut(s) 680, 908, 1137, 1210
BstPAI GACNNNNGTC 1 cut(s) 1011
BstSFI CTRYAG 2 cut(s) 41, 840
BstUI CGCG 3 cut(s) 249, 553, 898
BstV1I GCAGC 8 cut(s) 242, 825, 1108, 1111, 1354, 1584, 1587, 1821
BstV2I GAAGAC 3 cut(s) 117, 123, 416
BstX2I RGATCY 4 cut(s) 37, 139, 1144, 1598
BstYI RGATCY 4 cut(s) 37, 139, 1144, 1598
Bsu36I CCTNAGG 1 cut(s) 1219
BsuRI GGCC 5 cut(s) 188, 363, 474, 1225, 1378
BtgI CCRYGG 1 cut(s) 247
BtrI CACGTC 1 cut(s) 549
BtsCI GGATG 5 cut(s) 58, 74, 1604, 1780, 1787
BtsIMutI CAGTG 3 cut(s) 344, 1302, 1323
BtuMI TCGCGA 2 cut(s) 553, 898
BveI ACCTGC 1 cut(s) 800
Cac8I GCNNGC 6 cut(s) 235, 272, 633, 906, 1051, 1570
CaiI CAGNNNCTG 1 cut(s) 1226
CciI TCATGA 1 cut(s) 1859
CfoI GCGC 2 cut(s) 1212, 1746
Cfr10I RCCGGY 1 cut(s) 1117
Cfr13I GGNCC 6 cut(s) 173, 744, 1006, 1223, 1376, 1387
Cfr42I CCGCGG 1 cut(s) 250
CseI GACGC 1 cut(s) 137
Csp6I GTAC 4 cut(s) 680, 1080, 1783, 1848
CviAII CATG 7 cut(s) 677, 905, 1134, 1140, 1207, 1550, 1860
CviQI GTAC 4 cut(s) 680, 1080, 1783, 1848
DdeI CTNAG 7 cut(s) 64, 100, 242, 327, 1076, 1219, 1811
DpnI GATC 6 cut(s) 39, 141, 408, 727, 1146, 1600
DpnII GATC 6 cut(s) 37, 139, 406, 725, 1144, 1598
DraI TTTAAA 2 cut(s) 993, 1560
DraIII CACNNNGTG 1 cut(s) 19
Eam1104I CTCTTC 4 cut(s) 42, 1302, 1710, 1847
EarI CTCTTC 4 cut(s) 42, 1302, 1710, 1847
Ecl136II GAGCTC 1 cut(s) 324
Eco130I CCWWGG 2 cut(s) 1054, 1405
Eco24I GRGCYC 1 cut(s) 326
Eco31I GGTCTC 1 cut(s) 1811
Eco32I GATATC 1 cut(s) 1624
Eco47I GGWCC 4 cut(s) 173, 744, 1006, 1387
Eco53kI GAGCTC 1 cut(s) 324
Eco57I CTGAAG 3 cut(s) 1259, 1704, 1828
Eco72I CACGTG 1 cut(s) 1520
Eco81I CCTNAGG 1 cut(s) 1219
EcoICRI GAGCTC 1 cut(s) 324
EcoNI CCTNNNNNAGG 1 cut(s) 1795
EcoO109I RGGNCCY 4 cut(s) 173, 744, 1006, 1223
EcoRV GATATC 1 cut(s) 1624
EcoT14I CCWWGG 2 cut(s) 1054, 1405
EcoT22I ATGCAT 1 cut(s) 874
EcoT38I GRGCYC 1 cut(s) 326
ErhI CCWWGG 2 cut(s) 1054, 1405
FaeI CATG 7 cut(s) 680, 908, 1137, 1143, 1210, 1553, 1863
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FaqI GGGAC 5 cut(s) 186, 684, 757, 1019, 1373
FatI CATG 7 cut(s) 676, 904, 1133, 1139, 1206, 1549, 1859
FauNDI CATATG 1 cut(s) 958
FbaI TGATCA 1 cut(s) 406
FokI GGATG 5 cut(s) 61, 65, 1591, 1774, 1787
FriOI GRGCYC 1 cut(s) 326
FspBI CTAG 6 cut(s) 470, 683, 914, 984, 1055, 1706
GlaI GCGC 2 cut(s) 1211, 1745
GsaI CCCAGC 1 cut(s) 912
HaeII RGCGCY 1 cut(s) 1747
HaeIII GGCC 5 cut(s) 188, 363, 474, 1225, 1378
HapII CCGG 3 cut(s) 315, 1118, 1608
HgaI GACGC 1 cut(s) 137
HhaI GCGC 2 cut(s) 1212, 1746
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 7 cut(s) 680, 908, 1137, 1143, 1210, 1553, 1863
Hin6I GCGC 2 cut(s) 1210, 1744
HinP1I GCGC 2 cut(s) 1210, 1744
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 1 cut(s) 1853
HinfI GANTC 9 cut(s) 98, 200, 282, 343, 575, 589, 759, 1154, 1232
HpaII CCGG 3 cut(s) 315, 1118, 1608
HphI GGTGA 2 cut(s) 275, 416
Hpy166II GTNNAC 4 cut(s) 434, 568, 596, 1785
Hpy188III TCNNGA 9 cut(s) 26, 165, 182, 552, 723, 897, 1351, 1772, 1860
Hpy8I GTNNAC 4 cut(s) 434, 568, 596, 1785
Hpy99I CGWCG 2 cut(s) 92, 553
HpyAV CCTTC 8 cut(s) 282, 619, 823, 894, 1032, 1234, 1524, 1801
HpyCH4III ACNGT 5 cut(s) 404, 1270, 1318, 1540, 1738
HpyCH4IV ACGT 6 cut(s) 90, 548, 864, 1012, 1519, 1614
HpyF3I CTNAG 7 cut(s) 64, 100, 242, 327, 1076, 1219, 1811
HpySE526I ACGT 6 cut(s) 90, 548, 864, 1012, 1519, 1614
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 7 cut(s) 680, 908, 1137, 1143, 1210, 1553, 1863
HspAI GCGC 2 cut(s) 1210, 1744
KflI GGGWCCC 1 cut(s) 173
Ksp22I TGATCA 1 cut(s) 406
KspI CCGCGG 1 cut(s) 250
Kzo9I GATC 6 cut(s) 37, 139, 406, 725, 1144, 1598
LguI GCTCTTC 2 cut(s) 1302, 1710
LmnI GCTCC 1 cut(s) 628
Lsp1109I GCAGC 8 cut(s) 242, 825, 1108, 1111, 1354, 1584, 1587, 1821
LweI GCATC 9 cut(s) 31, 226, 465, 728, 812, 859, 881, 1337, 1688
MaeI CTAG 6 cut(s) 470, 683, 914, 984, 1055, 1706
MaeII ACGT 6 cut(s) 90, 548, 864, 1012, 1519, 1614
MaeIII GTNAC 3 cut(s) 263, 834, 1826
MalI GATC 6 cut(s) 39, 141, 408, 727, 1146, 1600
MboI GATC 6 cut(s) 37, 139, 406, 725, 1144, 1598
MfeI CAATTG 1 cut(s) 1335
MflI RGATCY 4 cut(s) 37, 139, 1144, 1598
MhlI GDGCHC 2 cut(s) 326, 853
MlyI GAGTC 2 cut(s) 92, 194
MmeI TCCRAC 1 cut(s) 73
Mph1103I ATGCAT 1 cut(s) 874
MroXI GAANNNNTTC 1 cut(s) 542
MseI TTAA 5 cut(s) 890, 992, 1559, 1749, 1796
MslI CAYNNNNRTG 1 cut(s) 1138
MspA1I CMGCKG 1 cut(s) 249
MspCI CTTAAG 1 cut(s) 1795
MspI CCGG 3 cut(s) 315, 1118, 1608
MunI CAATTG 1 cut(s) 1335
Mva1269I GAATGC 1 cut(s) 1273
MvnI CGCG 3 cut(s) 249, 553, 898
NdeI CATATG 1 cut(s) 958
NdeII GATC 6 cut(s) 37, 139, 406, 725, 1144, 1598
NlaIII CATG 7 cut(s) 680, 908, 1137, 1143, 1210, 1553, 1863
NlaIV GGNNCC 8 cut(s) 141, 174, 175, 745, 1007, 1377, 1389, 1546
NmuCI GTSAC 1 cut(s) 263
NruI TCGCGA 2 cut(s) 553, 898
NsiI ATGCAT 1 cut(s) 874
NspI RCATGY 4 cut(s) 680, 908, 1137, 1210
PaeI GCATGC 1 cut(s) 908
PagI TCATGA 1 cut(s) 1859
PciSI GCTCTTC 2 cut(s) 1302, 1710
PctI GAATGC 1 cut(s) 1273
PdmI GAANNNNTTC 1 cut(s) 542
PfeI GAWTC 7 cut(s) 282, 343, 575, 589, 759, 1154, 1232
PleI GAGTC 2 cut(s) 92, 194
PmaCI CACGTG 1 cut(s) 1520
PmlI CACGTG 1 cut(s) 1520
PpsI GAGTC 2 cut(s) 92, 194
Ppu21I YACGTR 1 cut(s) 1520
PpuMI RGGWCCY 3 cut(s) 173, 744, 1006
PshAI GACNNNNGTC 1 cut(s) 1011
Psp124BI GAGCTC 1 cut(s) 326
Psp5II RGGWCCY 3 cut(s) 173, 744, 1006
PspCI CACGTG 1 cut(s) 1520
PspFI CCCAGC 1 cut(s) 908
PspN4I GGNNCC 8 cut(s) 141, 174, 175, 745, 1007, 1377, 1389, 1546
PspPI GGNCC 6 cut(s) 173, 744, 1006, 1223, 1376, 1387
PspPPI RGGWCCY 3 cut(s) 173, 744, 1006
PstNI CAGNNNCTG 1 cut(s) 1226
PsuI RGATCY 4 cut(s) 37, 139, 1144, 1598
RruI TCGCGA 2 cut(s) 553, 898
RsaI GTAC 4 cut(s) 681, 1081, 1784, 1849
RsaNI GTAC 4 cut(s) 680, 1080, 1783, 1848
RseI CAYNNNNRTG 1 cut(s) 1138
SacI GAGCTC 1 cut(s) 326
SacII CCGCGG 1 cut(s) 250
SapI GCTCTTC 2 cut(s) 1302, 1710
SaqAI TTAA 5 cut(s) 890, 992, 1559, 1749, 1796
Sau3AI GATC 6 cut(s) 37, 139, 406, 725, 1144, 1598
Sau96I GGNCC 6 cut(s) 173, 744, 1006, 1223, 1376, 1387
SchI GAGTC 2 cut(s) 92, 194
SduI GDGCHC 2 cut(s) 326, 853
SfaNI GCATC 9 cut(s) 31, 226, 465, 728, 812, 859, 881, 1337, 1688
SfcI CTRYAG 2 cut(s) 41, 840
Sfr303I CCGCGG 1 cut(s) 250
SgrBI CCGCGG 1 cut(s) 250
SinI GGWCC 4 cut(s) 173, 744, 1006, 1387
SmiMI CAYNNNNRTG 1 cut(s) 1138
SmlI CTYRAG 2 cut(s) 599, 1795
SmoI CTYRAG 2 cut(s) 599, 1795
SpeI ACTAGT 1 cut(s) 1705
SphI GCATGC 1 cut(s) 908
SspMI CTAG 6 cut(s) 470, 683, 914, 984, 1055, 1706
SstI GAGCTC 1 cut(s) 326
StyI CCWWGG 2 cut(s) 1054, 1405
TaaI ACNGT 5 cut(s) 404, 1270, 1318, 1540, 1738
TaiI ACGT 6 cut(s) 93, 551, 867, 1015, 1522, 1617
TaqI TCGA 3 cut(s) 459, 697, 1476
TatI WGTACW 2 cut(s) 679, 1782
TauI GCSGC 5 cut(s) 249, 252, 255, 261, 305
TfiI GAWTC 7 cut(s) 282, 343, 575, 589, 759, 1154, 1232
Tru1I TTAA 5 cut(s) 890, 992, 1559, 1749, 1796
Tru9I TTAA 5 cut(s) 890, 992, 1559, 1749, 1796
TscAI CASTG 3 cut(s) 344, 1309, 1323
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 8 cut(s) 255, 813, 1121, 1124, 1367, 1572, 1575, 1834
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 5 cut(s) 69, 1253, 1272, 1593, 1848
TspGWI ACGGA 3 cut(s) 67, 152, 1097
TspRI CASTG 3 cut(s) 344, 1309, 1323
Vha464I CTTAAG 1 cut(s) 1795
VpaK11BI GGWCC 4 cut(s) 173, 744, 1006, 1387
XagI CCTNNNNNAGG 1 cut(s) 1795
XapI RAATTY 4 cut(s) 693, 882, 994, 1354
XceI RCATGY 4 cut(s) 680, 908, 1137, 1210
XmaJI CCTAGG 1 cut(s) 1054
XmnI GAANNNNTTC 1 cut(s) 542
XspI CTAG 6 cut(s) 470, 683, 914, 984, 1055, 1706
Zsp2I ATGCAT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.