AT5G06270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010896
Physical Location & Seq
Forward (+)
1912013 .. 1913728
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G06270.1

Sequence Viewer

Length: 369 bp
ATGAGTCGAAGAAGTCCAAAGCTGGAACTGAAGTTGAATCTCTCGCCGCCTACGTCAAGCCAGAGGAGGATGGTTCGATCTCCAAGCCGCTCAGCGACGACTTCACCGACCTCACCTCCAAGCTCATGCGTCTCGTCGGAGATGAACCAGGACGAGCCATCTGTGAGATACTCGACAAGTCCAGAGACTACCTCGATGGTTCTTGTCGGATGTCCTCGTTGTCTCATGTACGTTATGCTCTCAGAAGACGACCCTAAATGCCCTAAATGCAAAAGCACCGTTCTTCTTGATTTCCTCCATGAAAACGCCACAAACGCCAACGCAAACGCAGCCGCTGCTTCCTCTGGTCGCAAGACTCGAAGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

122

Amino Acids

13.31

Weight (kDa)

9.54

Isoelectric Point (pI)

91.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn-ribbon_GIR1 PF24747 64 - 100 6.5e-29 GIR1-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 90
AciI CCGC 3 cut(s) 47, 88, 333
AcuI CTGAAG 1 cut(s) 50
AfaI GTAC 1 cut(s) 230
AgsI TTSAA 1 cut(s) 37
AjnI CCWGG 1 cut(s) 147
AluBI AGCT 2 cut(s) 22, 123
AluI AGCT 2 cut(s) 22, 123
Alw26I GTCTC 3 cut(s) 136, 179, 227
AlwNI CAGNNNCTG 1 cut(s) 335
ApeKI GCWGC 2 cut(s) 329, 335
AsuHPI GGTGA 2 cut(s) 96, 105
BbsI GAAGAC 1 cut(s) 252
BbvI GCAGC 2 cut(s) 322, 341
BccI CCATC 3 cut(s) 64, 166, 190
BciT130I CCWGG 1 cut(s) 149
BcoDI GTCTC 3 cut(s) 136, 179, 227
BisI GCNGC 5 cut(s) 47, 88, 330, 333, 336
BlpI GCTNAGC 1 cut(s) 91
BlsI GCNGC 5 cut(s) 48, 89, 331, 334, 337
Bme1390I CCNGG 1 cut(s) 149
BmrFI CCNGG 1 cut(s) 149
BpiI GAAGAC 1 cut(s) 252
BplI GAGNNNNNCTC 2 cut(s) 176, 208
Bpu1102I GCTNAGC 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
BseBI CCWGG 1 cut(s) 149
BseGI GGATG 2 cut(s) 75, 215
BseMII CTCAG 2 cut(s) 105, 255
BseRI GAGGAG 1 cut(s) 79
BseXI GCAGC 2 cut(s) 322, 341
BsmAI GTCTC 3 cut(s) 136, 179, 227
BsmBI CGTCTC 1 cut(s) 136
Bsp143I GATC 1 cut(s) 77
Bsp1720I GCTNAGC 1 cut(s) 91
BspACI CCGC 3 cut(s) 47, 88, 333
BspCNI CTCAG 2 cut(s) 104, 254
BsrBI CCGCTC 1 cut(s) 90
BssMI GATC 1 cut(s) 77
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 1 cut(s) 280
BstAPI GCANNNNNTGC 1 cut(s) 335
BstDEI CTNAG 2 cut(s) 91, 241
BstF5I GGATG 2 cut(s) 75, 215
BstKTI GATC 1 cut(s) 80
BstMAI GTCTC 3 cut(s) 136, 179, 227
BstMBI GATC 1 cut(s) 77
BstMWI GCNNNNNNNGC 4 cut(s) 267, 314, 329, 335
BstNI CCWGG 1 cut(s) 149
BstSCI CCNGG 1 cut(s) 147
BstV1I GCAGC 2 cut(s) 322, 341
BstV2I GAAGAC 1 cut(s) 252
BtsCI GGATG 2 cut(s) 75, 215
CaiI CAGNNNCTG 1 cut(s) 335
CseI GACGC 1 cut(s) 118
Csp6I GTAC 1 cut(s) 229
CviAII CATG 3 cut(s) 126, 226, 299
CviJI RGCY 6 cut(s) 22, 60, 87, 123, 157, 332
CviKI_1 RGCY 6 cut(s) 22, 60, 87, 123, 157, 332
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 2 cut(s) 91, 241
DpnI GATC 1 cut(s) 79
DpnII GATC 1 cut(s) 77
Eco57I CTGAAG 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 147
Esp3I CGTCTC 1 cut(s) 136
FaeI CATG 3 cut(s) 129, 229, 302
FaiI YATR 4 cut(s) 127, 227, 236, 300
FatI CATG 3 cut(s) 125, 225, 298
Fnu4HI GCNGC 5 cut(s) 47, 88, 330, 333, 336
FokI GGATG 2 cut(s) 82, 222
Fsp4HI GCNGC 5 cut(s) 47, 88, 330, 333, 336
GluI GCNGC 5 cut(s) 47, 88, 330, 333, 336
HgaI GACGC 1 cut(s) 118
Hin1II CATG 3 cut(s) 129, 229, 302
HinfI GANTC 3 cut(s) 4, 37, 355
HphI GGTGA 2 cut(s) 96, 105
Hpy188I TCNGA 3 cut(s) 139, 209, 244
Hpy188III TCNNGA 2 cut(s) 182, 287
Hpy99I CGWCG 2 cut(s) 100, 139
HpyAV CCTTC 1 cut(s) 354
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 2 cut(s) 53, 231
HpyCH4V TGCA 1 cut(s) 270
HpyF10VI GCNNNNNNNGC 4 cut(s) 267, 314, 329, 335
HpyF3I CTNAG 2 cut(s) 91, 241
HpySE526I ACGT 2 cut(s) 53, 231
Hsp92II CATG 3 cut(s) 129, 229, 302
Kzo9I GATC 1 cut(s) 77
LpnPI CCDG 6 cut(s) 8, 74, 134, 161, 195, 330
Lsp1109I GCAGC 2 cut(s) 322, 341
MaeII ACGT 2 cut(s) 53, 231
MalI GATC 1 cut(s) 79
MbiI CCGCTC 1 cut(s) 90
MboI GATC 1 cut(s) 77
MboII GAAGA 3 cut(s) 21, 257, 275
MlyI GAGTC 2 cut(s) 13, 349
MmeI TCCRAC 2 cut(s) 117, 187
MnlI CCTC 8 cut(s) 57, 60, 121, 126, 202, 225, 305, 352
MspA1I CMGCKG 1 cut(s) 335
MspR9I CCNGG 1 cut(s) 149
MvaI CCWGG 1 cut(s) 149
MwoI GCNNNNNNNGC 4 cut(s) 267, 314, 329, 335
NdeII GATC 1 cut(s) 77
NlaIII CATG 3 cut(s) 129, 229, 302
PcsI WCGNNNNNNNCGW 3 cut(s) 50, 104, 355
PfeI GAWTC 1 cut(s) 37
PkrI GCNGC 5 cut(s) 48, 89, 331, 334, 337
PleI GAGTC 2 cut(s) 12, 349
PpsI GAGTC 2 cut(s) 12, 349
Psp6I CCWGG 1 cut(s) 147
PspGI CCWGG 1 cut(s) 147
PstNI CAGNNNCTG 1 cut(s) 335
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
SatI GCNGC 5 cut(s) 47, 88, 330, 333, 336
Sau3AI GATC 1 cut(s) 77
SchI GAGTC 2 cut(s) 13, 349
ScrFI CCNGG 1 cut(s) 149
SetI ASST 7 cut(s) 24, 56, 113, 118, 125, 194, 234
SsiI CCGC 3 cut(s) 47, 88, 333
StyD4I CCNGG 1 cut(s) 147
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 2 cut(s) 56, 234
TaqI TCGA 5 cut(s) 7, 76, 173, 194, 358
TauI GCSGC 3 cut(s) 49, 90, 335
TfiI GAWTC 1 cut(s) 37
TseI GCWGC 2 cut(s) 329, 335
TspDTI ATGAA 2 cut(s) 158, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.