MD12G1033200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010896
Physical Location & Seq
Forward (+)
3672340 .. 3673760
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1033200.v1.1.491

Sequence Viewer

Length: 336 bp
ATGAGTAGACGGAACGGAGGCGGTCCGAAGCTTGACCTGAAGCTAAACCTGTCACCACCGAGGGCTAATCATCAGCTGGCGGAGTCACCACGAAGATCAGCGACTGTTTCACCTACGTCACCGCCGAGCTCATGCGTGTCATCGGAGCTGAATCAAGACGACAATTTTAGCCCAGAGACCATTTCCATGGTGCTAGTCGGGTGCCCTAGGTGCCTCATGTATGTGATGCTCTCGGAGGACGACCCCAAATGCCCTAAATGCAAGAGCACTGTTTTGCTAGACTTTCTTCATGACACCACTACCACCACACCAACCTCAAAGACAAGGAAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

112

Amino Acids

12.11

Weight (kDa)

8.69

Isoelectric Point (pI)

71.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn-ribbon_GIR1 PF24747 62 - 97 2.9e-28 GIR1-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 201, 210
AccI GTMKAC 1 cut(s) 7
AciI CCGC 3 cut(s) 21, 80, 122
AcuI CTGAAG 1 cut(s) 59
AluBI AGCT 6 cut(s) 31, 43, 76, 129, 148, 333
AluI AGCT 6 cut(s) 31, 43, 76, 129, 148, 333
Alw21I GWGCWC 2 cut(s) 131, 269
Alw26I GTCTC 1 cut(s) 170
AlwNI CAGNNNCTG 1 cut(s) 104
AspA2I CCTAGG 1 cut(s) 206
AspS9I GGNCC 1 cut(s) 23
AsuHPI GGTGA 4 cut(s) 45, 78, 102, 111
AvaII GGWCC 1 cut(s) 23
AvrII CCTAGG 1 cut(s) 206
BaeGI GKGCMC 1 cut(s) 206
BanI GGYRCC 2 cut(s) 201, 210
BanII GRGCYC 1 cut(s) 131
Bbv12I GWGCWC 2 cut(s) 131, 269
BcoDI GTCTC 1 cut(s) 170
BfaI CTAG 3 cut(s) 194, 207, 278
BlnI CCTAGG 1 cut(s) 206
Bme18I GGWCC 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 2 cut(s) 203, 212
BmsI GCATC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 3 cut(s) 59, 186, 206
BseDI CCNNGG 3 cut(s) 59, 186, 206
BseSI GKGCMC 1 cut(s) 206
BshNI GGYRCC 2 cut(s) 201, 210
BsiHKAI GWGCWC 2 cut(s) 131, 269
BsmAI GTCTC 1 cut(s) 170
Bso31I GGTCTC 1 cut(s) 170
Bsp1286I GDGCHC 3 cut(s) 131, 206, 269
Bsp143I GATC 1 cut(s) 95
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 3 cut(s) 21, 80, 122
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 2 cut(s) 203, 212
BspQI GCTCTTC 1 cut(s) 323
BspT107I GGYRCC 2 cut(s) 201, 210
BspTNI GGTCTC 1 cut(s) 170
BssECI CCNNGG 3 cut(s) 59, 186, 206
BssMI GATC 1 cut(s) 95
BssT1I CCWWGG 2 cut(s) 186, 206
Bst4CI ACNGT 2 cut(s) 106, 271
Bst6I CTCTTC 1 cut(s) 323
BstC8I GCNNGC 1 cut(s) 78
BstDSI CCRYGG 1 cut(s) 186
BstKTI GATC 1 cut(s) 98
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 1 cut(s) 95
BstMWI GCNNNNNNNGC 2 cut(s) 210, 258
BstSLI GKGCMC 1 cut(s) 206
BstXI CCANNNNNNTGG 1 cut(s) 187
BtgI CCRYGG 1 cut(s) 186
BtsIMutI CAGTG 1 cut(s) 267
Cac8I GCNNGC 1 cut(s) 78
CaiI CAGNNNCTG 1 cut(s) 104
CciI TCATGA 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 23
CpoI CGGWCCG 1 cut(s) 23
CspI CGGWCCG 1 cut(s) 23
CviAII CATG 4 cut(s) 132, 187, 217, 290
CviJI RGCY 8 cut(s) 31, 43, 65, 76, 129, 148, 171, 333
CviKI_1 RGCY 8 cut(s) 31, 43, 65, 76, 129, 148, 171, 333
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 323
EciI GGCGGA 1 cut(s) 95
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 186, 206
Eco24I GRGCYC 1 cut(s) 131
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 1 cut(s) 23
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 1 cut(s) 59
EcoICRI GAGCTC 1 cut(s) 129
EcoT14I CCWWGG 2 cut(s) 186, 206
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 2 cut(s) 186, 206
FaeI CATG 4 cut(s) 135, 190, 220, 293
FaiI YATR 5 cut(s) 133, 188, 218, 222, 291
FatI CATG 4 cut(s) 131, 186, 216, 289
FblI GTMKAC 1 cut(s) 7
FriOI GRGCYC 1 cut(s) 131
FspBI CTAG 3 cut(s) 194, 207, 278
Hin1II CATG 4 cut(s) 135, 190, 220, 293
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 2 cut(s) 83, 151
HphI GGTGA 4 cut(s) 45, 78, 102, 111
Hpy166II GTNNAC 1 cut(s) 8
Hpy188I TCNGA 3 cut(s) 27, 145, 235
Hpy188III TCNNGA 2 cut(s) 155, 290
Hpy8I GTNNAC 1 cut(s) 8
HpyCH4III ACNGT 2 cut(s) 106, 271
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 2 cut(s) 210, 258
HpySE526I ACGT 1 cut(s) 116
Hsp92II CATG 4 cut(s) 135, 190, 220, 293
Kzo9I GATC 1 cut(s) 95
LguI GCTCTTC 1 cut(s) 323
LmnI GCTCC 1 cut(s) 145
LpnPI CCDG 4 cut(s) 50, 62, 62, 186
LweI GCATC 1 cut(s) 216
MaeI CTAG 3 cut(s) 194, 207, 278
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 3 cut(s) 51, 84, 117
MalI GATC 1 cut(s) 97
MboI GATC 1 cut(s) 95
MboII GAAGA 2 cut(s) 105, 278
MhlI GDGCHC 3 cut(s) 131, 206, 269
MluCI AATT 1 cut(s) 163
MlyI GAGTC 1 cut(s) 92
MnlI CCTC 5 cut(s) 11, 54, 224, 229, 325
MslI CAYNNNNRTG 2 cut(s) 185, 221
MspA1I CMGCKG 1 cut(s) 76
MwoI GCNNNNNNNGC 2 cut(s) 210, 258
NcoI CCATGG 1 cut(s) 186
NdeII GATC 1 cut(s) 95
NlaIII CATG 4 cut(s) 135, 190, 220, 293
NlaIV GGNNCC 2 cut(s) 203, 212
NmeAIII GCCGAG 1 cut(s) 150
NmuCI GTSAC 3 cut(s) 51, 84, 117
PagI TCATGA 1 cut(s) 289
PciSI GCTCTTC 1 cut(s) 323
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PfeI GAWTC 1 cut(s) 151
PleI GAGTC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 91
Psp124BI GAGCTC 1 cut(s) 131
PspN4I GGNNCC 2 cut(s) 203, 212
PspPI GGNCC 1 cut(s) 23
PstNI CAGNNNCTG 1 cut(s) 104
PvuII CAGCTG 1 cut(s) 76
RseI CAYNNNNRTG 2 cut(s) 185, 221
Rsr2I CGGWCCG 1 cut(s) 23
RsrII CGGWCCG 1 cut(s) 23
SacI GAGCTC 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 323
Sau3AI GATC 1 cut(s) 95
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 1 cut(s) 92
SduI GDGCHC 3 cut(s) 131, 206, 269
SfaNI GCATC 1 cut(s) 216
SinI GGWCC 1 cut(s) 23
SmiMI CAYNNNNRTG 2 cut(s) 185, 221
Sse9I AATT 1 cut(s) 163
SsiI CCGC 3 cut(s) 21, 80, 122
SspMI CTAG 3 cut(s) 194, 207, 278
SstI GAGCTC 1 cut(s) 131
StyI CCWWGG 2 cut(s) 186, 206
TaaI ACNGT 2 cut(s) 106, 271
TaiI ACGT 1 cut(s) 119
TasI AATT 1 cut(s) 163
TfiI GAWTC 1 cut(s) 151
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 3 cut(s) 51, 84, 117
Tsp45I GTSAC 3 cut(s) 51, 84, 117
TspDTI ATGAA 1 cut(s) 278
TspGWI ACGGA 2 cut(s) 25, 30
TspRI CASTG 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 23
XmaJI CCTAGG 1 cut(s) 206
XmiI GTMKAC 1 cut(s) 7
XspI CTAG 3 cut(s) 194, 207, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.