MD11G1170900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010896
Physical Location & Seq
Reverse (-)
18521167 .. 18521937
771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1170900.v1.1.491

Sequence Viewer

Length: 333 bp
ATGAGCAGAAGAGGAAGTAGTAGTCCAAAGCTTGAATTGAAGCTAAATCTGTCTCCTCCAAGTGCTAACTCATCACCAATTGACACCTCAAATGCCTCCGAGTCATCCTCGGACATGTTAATCGACCGCAGCTCATACGTGTCGTCAGAACCAGACGATGAGACGATGCATTGCCTGAGCAGGCCCGAAGCCAAGGCGAGGGTGCTTGCAGGGTGTCCTCGGTGCCTCATGTATGTCATGCTATCTGAATTGGATCCAAACTGCCCAAAATGCAAAAGCTCTGTCTTGCTCGATTTCCTTAACGAAGAAAGCACCAAGAGGAAAGGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

111

Amino Acids

12.04

Weight (kDa)

5.81

Isoelectric Point (pI)

82.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn-ribbon_GIR1 PF24747 68 - 100 8.4e-22 GIR1-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 222
AciI CCGC 1 cut(s) 127
AclWI GGATC 2 cut(s) 248, 261
AfiI CCNNNNNNNGG 1 cut(s) 198
AflIII ACRYGT 2 cut(s) 114, 138
AgsI TTSAA 2 cut(s) 35, 40
AluBI AGCT 4 cut(s) 31, 43, 132, 279
AluI AGCT 4 cut(s) 31, 43, 132, 279
Alw26I GTCTC 2 cut(s) 57, 155
AlwI GGATC 2 cut(s) 248, 261
AoxI GGCC 1 cut(s) 182
ApeKI GCWGC 1 cut(s) 129
ArsI GACNNNNNNTTYG 2 cut(s) 267, 299
AspS9I GGNCC 1 cut(s) 183
AsuHPI GGTGA 1 cut(s) 66
BamHI GGATCC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 141
BcoDI GTCTC 2 cut(s) 57, 155
BisI GCNGC 1 cut(s) 130
BlsI GCNGC 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 183
BmiI GGNNCC 2 cut(s) 224, 255
BmsI GCATC 1 cut(s) 156
BplI GAGNNNNNCTC 2 cut(s) 92, 124
Bpu10I CCTNAGC 1 cut(s) 176
BsaAI YACGTR 1 cut(s) 139
BsaJI CCNNGG 3 cut(s) 108, 192, 218
Bsc4I CCNNNNNNNGG 1 cut(s) 198
Bse3DI GCAATG 1 cut(s) 169
BseDI CCNNGG 3 cut(s) 108, 192, 218
BseGI GGATG 1 cut(s) 104
BseLI CCNNNNNNNGG 1 cut(s) 198
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 1 cut(s) 167
BseRI GAGGAG 1 cut(s) 45
BseXI GCAGC 1 cut(s) 141
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 1 cut(s) 184
BshNI GGYRCC 1 cut(s) 222
BsiEI CGRYCG 1 cut(s) 127
BslI CCNNNNNNNGG 1 cut(s) 198
BsmAI GTCTC 2 cut(s) 57, 155
BsmBI CGTCTC 1 cut(s) 155
BsnI GGCC 1 cut(s) 184
Bsp143I GATC 1 cut(s) 253
BspACI CCGC 1 cut(s) 127
BspANI GGCC 1 cut(s) 184
BspCNI CTCAG 1 cut(s) 168
BspLI GGNNCC 2 cut(s) 224, 255
BspPI GGATC 2 cut(s) 248, 261
BspT107I GGYRCC 1 cut(s) 222
BsrDI GCAATG 1 cut(s) 169
BssECI CCNNGG 3 cut(s) 108, 192, 218
BssMI GATC 1 cut(s) 253
BssT1I CCWWGG 1 cut(s) 192
Bst6I CTCTTC 1 cut(s) 4
BstBAI YACGTR 1 cut(s) 139
BstC8I GCNNGC 2 cut(s) 182, 207
BstDEI CTNAG 1 cut(s) 176
BstF5I GGATG 1 cut(s) 104
BstKTI GATC 1 cut(s) 256
BstMAI GTCTC 2 cut(s) 57, 155
BstMBI GATC 1 cut(s) 253
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 1 cut(s) 270
BstNSI RCATGY 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 1 cut(s) 184
BtsCI GGATG 1 cut(s) 104
Cac8I GCNNGC 2 cut(s) 182, 207
Cfr13I GGNCC 1 cut(s) 183
CviAII CATG 3 cut(s) 115, 229, 238
CviJI RGCY 6 cut(s) 31, 43, 132, 184, 191, 279
CviKI_1 RGCY 6 cut(s) 31, 43, 132, 184, 191, 279
DdeI CTNAG 1 cut(s) 176
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 4
EarI CTCTTC 1 cut(s) 4
Eco130I CCWWGG 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 192
EcoT22I ATGCAT 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 192
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 3 cut(s) 118, 232, 241
FaiI YATR 5 cut(s) 116, 136, 230, 234, 239
FatI CATG 3 cut(s) 114, 228, 237
Fnu4HI GCNGC 1 cut(s) 130
FokI GGATG 1 cut(s) 91
Fsp4HI GCNGC 1 cut(s) 130
GluI GCNGC 1 cut(s) 130
HaeIII GGCC 1 cut(s) 184
Hin1II CATG 3 cut(s) 118, 232, 241
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 1 cut(s) 101
HphI GGTGA 1 cut(s) 66
Hpy188I TCNGA 4 cut(s) 100, 112, 148, 247
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 3 cut(s) 169, 209, 273
HpyF10VI GCNNNNNNNGC 1 cut(s) 270
HpyF3I CTNAG 1 cut(s) 176
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 3 cut(s) 118, 232, 241
Kzo9I GATC 1 cut(s) 253
LpnPI CCDG 4 cut(s) 165, 166, 188, 195
Lsp1109I GCAGC 1 cut(s) 141
LweI GCATC 1 cut(s) 156
MaeII ACGT 1 cut(s) 138
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MboII GAAGA 2 cut(s) 21, 317
MfeI CAATTG 1 cut(s) 78
MflI RGATCY 1 cut(s) 253
MluCI AATT 3 cut(s) 35, 78, 248
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 9 cut(s) 5, 66, 97, 106, 118, 192, 228, 236, 312
Mph1103I ATGCAT 1 cut(s) 171
MseI TTAA 2 cut(s) 119, 300
MunI CAATTG 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 270
NdeII GATC 1 cut(s) 253
NlaIII CATG 3 cut(s) 118, 232, 241
NlaIV GGNNCC 2 cut(s) 224, 255
NsiI ATGCAT 1 cut(s) 171
NspI RCATGY 1 cut(s) 118
PciI ACATGT 1 cut(s) 114
PkrI GCNGC 1 cut(s) 131
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
Ppu21I YACGTR 1 cut(s) 139
PscI ACATGT 1 cut(s) 114
PspN4I GGNNCC 2 cut(s) 224, 255
PspPI GGNCC 1 cut(s) 183
PsuI RGATCY 1 cut(s) 253
SaqAI TTAA 2 cut(s) 119, 300
SatI GCNGC 1 cut(s) 130
Sau3AI GATC 1 cut(s) 253
Sau96I GGNCC 1 cut(s) 183
SchI GAGTC 1 cut(s) 110
SetI ASST 6 cut(s) 33, 45, 89, 134, 141, 281
SfaNI GCATC 1 cut(s) 156
Sse9I AATT 3 cut(s) 35, 78, 248
SsiI CCGC 1 cut(s) 127
StyI CCWWGG 1 cut(s) 192
TaiI ACGT 1 cut(s) 141
TaqI TCGA 2 cut(s) 123, 291
TasI AATT 3 cut(s) 35, 78, 248
Tru1I TTAA 2 cut(s) 119, 300
Tru9I TTAA 2 cut(s) 119, 300
TseI GCWGC 1 cut(s) 129
XceI RCATGY 1 cut(s) 118
Zsp2I ATGCAT 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.