pycom03g10550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010896
Physical Location & Seq
Forward (+)
9761176 .. 9761506
331 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g10550.1

Sequence Viewer

Length: 279 bp
ATGAGCAGACGCGGAAGTAGTGGTCCAAGGCTTGAATTGAAGCTGAACCTATCTCCTCCGAGGGCTAACTCATCGTCACCAGCTCATGCGTGTCATTGGAGCCTGATCGATCAGATGATGCATTACCTGAGTACGCCCGAAGCCAAGGCGATGGTGCTCGCAGGGTGTCCTCGGTGCCTCATGTATGTCATGCTATCTGAAGTGGATCCAAAGTGCCCCAAATGCAAAAGCTCTGTCTTGCTCAATTTCTTTAGCGAAGAAAGCAGCAAAAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

93

Amino Acids

10.28

Weight (kDa)

9.36

Isoelectric Point (pI)

86.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn-ribbon_GIR1 PF24747 50 - 84 2.3e-22 GIR1-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 174
AccII CGCG 1 cut(s) 12
AciI CCGC 1 cut(s) 12
AclWI GGATC 2 cut(s) 200, 213
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 1 cut(s) 133
AgsI TTSAA 2 cut(s) 35, 40
AluBI AGCT 3 cut(s) 43, 83, 231
AluI AGCT 3 cut(s) 43, 83, 231
Alw21I GWGCWC 1 cut(s) 159
AlwI GGATC 2 cut(s) 200, 213
ApeKI GCWGC 1 cut(s) 264
ArsI GACNNNNNNTTYG 2 cut(s) 219, 251
AspS9I GGNCC 1 cut(s) 23
AsuHPI GGTGA 1 cut(s) 69
AvaII GGWCC 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 218
BamHI GGATCC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 159
BccI CCATC 1 cut(s) 145
BisI GCNGC 1 cut(s) 265
BlsI GCNGC 1 cut(s) 266
Bme18I GGWCC 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 3 cut(s) 101, 176, 207
BmsI GCATC 1 cut(s) 108
Bsa29I ATCGAT 1 cut(s) 108
BsaJI CCNNGG 4 cut(s) 26, 59, 144, 170
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 4 cut(s) 26, 59, 144, 170
BseMII CTCAG 1 cut(s) 119
BseRI GAGGAG 1 cut(s) 45
BseSI GKGCMC 1 cut(s) 218
Bsh1236I CGCG 1 cut(s) 12
BshNI GGYRCC 1 cut(s) 174
BshVI ATCGAT 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 159
Bsp1286I GDGCHC 2 cut(s) 159, 218
Bsp143I GATC 3 cut(s) 105, 109, 205
BspACI CCGC 1 cut(s) 12
BspCNI CTCAG 1 cut(s) 120
BspDI ATCGAT 1 cut(s) 108
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 3 cut(s) 101, 176, 207
BspPI GGATC 2 cut(s) 200, 213
BspT107I GGYRCC 1 cut(s) 174
BssECI CCNNGG 4 cut(s) 26, 59, 144, 170
BssMI GATC 3 cut(s) 105, 109, 205
BssT1I CCWWGG 2 cut(s) 26, 144
BstC8I GCNNGC 1 cut(s) 159
BstDEI CTNAG 1 cut(s) 128
BstFNI CGCG 1 cut(s) 12
BstKTI GATC 3 cut(s) 108, 112, 208
BstMBI GATC 3 cut(s) 105, 109, 205
BstMWI GCNNNNNNNGC 2 cut(s) 222, 261
BstSLI GKGCMC 1 cut(s) 218
BstUI CGCG 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 205
BstXI CCANNNNNNTGG 1 cut(s) 151
BstYI RGATCY 1 cut(s) 205
Bsu15I ATCGAT 1 cut(s) 108
BsuTUI ATCGAT 1 cut(s) 108
BtgZI GCGATG 1 cut(s) 164
Cac8I GCNNGC 1 cut(s) 159
Cfr13I GGNCC 1 cut(s) 23
ClaI ATCGAT 1 cut(s) 108
CseI GACGC 1 cut(s) 18
Csp6I GTAC 1 cut(s) 132
CviAII CATG 3 cut(s) 86, 181, 190
CviJI RGCY 7 cut(s) 31, 43, 65, 83, 102, 143, 231
CviKI_1 RGCY 7 cut(s) 31, 43, 65, 83, 102, 143, 231
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 1 cut(s) 128
DpnI GATC 3 cut(s) 107, 111, 207
DpnII GATC 3 cut(s) 105, 109, 205
Eco130I CCWWGG 2 cut(s) 26, 144
Eco47I GGWCC 1 cut(s) 23
Eco57I CTGAAG 1 cut(s) 219
EcoT14I CCWWGG 2 cut(s) 26, 144
EcoT22I ATGCAT 1 cut(s) 123
ErhI CCWWGG 2 cut(s) 26, 144
FaeI CATG 3 cut(s) 89, 184, 193
FaiI YATR 4 cut(s) 87, 182, 186, 191
FatI CATG 3 cut(s) 85, 180, 189
Fnu4HI GCNGC 1 cut(s) 265
Fsp4HI GCNGC 1 cut(s) 265
GluI GCNGC 1 cut(s) 265
HgaI GACGC 1 cut(s) 18
Hin1II CATG 3 cut(s) 89, 184, 193
HphI GGTGA 1 cut(s) 69
Hpy188I TCNGA 3 cut(s) 60, 114, 199
HpyCH4V TGCA 2 cut(s) 121, 225
HpyF10VI GCNNNNNNNGC 2 cut(s) 222, 261
HpyF3I CTNAG 1 cut(s) 128
Hsp92II CATG 3 cut(s) 89, 184, 193
Kzo9I GATC 3 cut(s) 105, 109, 205
LmnI GCTCC 1 cut(s) 99
LpnPI CCDG 4 cut(s) 93, 116, 140, 147
LweI GCATC 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 75
MalI GATC 3 cut(s) 107, 111, 207
MboI GATC 3 cut(s) 105, 109, 205
MboII GAAGA 1 cut(s) 269
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 2 cut(s) 159, 218
MluCI AATT 2 cut(s) 35, 244
MnlI CCTC 4 cut(s) 54, 66, 180, 188
Mph1103I ATGCAT 1 cut(s) 123
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 2 cut(s) 222, 261
NdeII GATC 3 cut(s) 105, 109, 205
NlaIII CATG 3 cut(s) 89, 184, 193
NlaIV GGNNCC 3 cut(s) 101, 176, 207
NmuCI GTSAC 1 cut(s) 75
NsiI ATGCAT 1 cut(s) 123
PkrI GCNGC 1 cut(s) 266
PspN4I GGNNCC 3 cut(s) 101, 176, 207
PspPI GGNCC 1 cut(s) 23
PsuI RGATCY 1 cut(s) 205
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
SatI GCNGC 1 cut(s) 265
Sau3AI GATC 3 cut(s) 105, 109, 205
Sau96I GGNCC 1 cut(s) 23
SduI GDGCHC 2 cut(s) 159, 218
SetI ASST 5 cut(s) 45, 51, 85, 129, 233
SfaNI GCATC 1 cut(s) 108
SinI GGWCC 1 cut(s) 23
Sse9I AATT 2 cut(s) 35, 244
SsiI CCGC 1 cut(s) 12
StyI CCWWGG 2 cut(s) 26, 144
TaqI TCGA 1 cut(s) 108
TasI AATT 2 cut(s) 35, 244
TseFI GTSAC 1 cut(s) 75
TseI GCWGC 1 cut(s) 264
Tsp45I GTSAC 1 cut(s) 75
VpaK11BI GGWCC 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.