MD14G1034000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010896
Physical Location & Seq
Forward (+)
3017637 .. 3018377
741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1034000.v1.1.491

Sequence Viewer

Length: 375 bp
ATGTCACACACCACTTTATTATTTTCACTGTTTTCCATGAGCAGGCGAAACGGAGGCGGTCCGAAGCTTGACCTAAAGCTGAACCTGTCACCACCGAGGGCTAATCAGCAACTGGCAGAGTCACCACGAAGATCGGCAACTGTTTCACCAACGTCACCGCCAAGCTCATGCGTGTCATCGGAGCTGAATCAGGATGACAATTTCAGCCCGGAGACCACTTCCATGGTGCTAGTCGGATGCCCTAGGTGCCTCATGTATGTGATGCTCTCGGAGGACGACCCTAAATGCCCTAAATGCAAGAGCACTGTTTTGCTAGACTTTCTTCATGACACCACCACCACCACCACCCCAACCTCAAGGACAAGGAGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.64

Weight (kDa)

8.72

Isoelectric Point (pI)

75.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn-ribbon_GIR1 PF24747 73 - 109 6.7e-29 GIR1-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 246
AciI CCGC 2 cut(s) 57, 158
AfiI CCNNNNNNNGG 1 cut(s) 42
AluBI AGCT 4 cut(s) 67, 79, 165, 184
AluI AGCT 4 cut(s) 67, 79, 165, 184
Alw21I GWGCWC 1 cut(s) 305
Alw26I GTCTC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 112
AspA2I CCTAGG 1 cut(s) 242
AspS9I GGNCC 1 cut(s) 59
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 4 cut(s) 81, 114, 138, 147
AvaII GGWCC 1 cut(s) 59
AvrII CCTAGG 1 cut(s) 242
BanI GGYRCC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 305
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 3 cut(s) 230, 243, 314
BlnI CCTAGG 1 cut(s) 242
Bme1390I CCNGG 1 cut(s) 209
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 59
BmiI GGNNCC 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 209
BmsI GCATC 2 cut(s) 227, 252
BpuEI CTTGAG 1 cut(s) 340
BpuMI CCSGG 1 cut(s) 209
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 3 cut(s) 95, 222, 242
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse1I ACTGG 1 cut(s) 117
BseDI CCNNGG 3 cut(s) 95, 222, 242
BseGI GGATG 2 cut(s) 199, 242
BseLI CCNNNNNNNGG 1 cut(s) 42
BseNI ACTGG 1 cut(s) 117
BshNI GGYRCC 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 305
BsiSI CCGG 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 1 cut(s) 206
Bso31I GGTCTC 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 305
Bsp143I GATC 1 cut(s) 131
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 2 cut(s) 57, 158
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 206
BsrI ACTGG 1 cut(s) 117
BssECI CCNNGG 3 cut(s) 95, 222, 242
BssMI GATC 1 cut(s) 131
BssT1I CCWWGG 2 cut(s) 222, 242
Bst4CI ACNGT 3 cut(s) 30, 142, 307
BstC8I GCNNGC 1 cut(s) 44
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 2 cut(s) 199, 242
BstKTI GATC 1 cut(s) 134
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 1 cut(s) 131
BstMWI GCNNNNNNNGC 2 cut(s) 246, 294
BstSCI CCNGG 1 cut(s) 207
BstXI CCANNNNNNTGG 1 cut(s) 223
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 2 cut(s) 199, 242
BtsIMutI CAGTG 2 cut(s) 26, 303
Cac8I GCNNGC 1 cut(s) 44
CaiI CAGNNNCTG 1 cut(s) 112
CciI TCATGA 1 cut(s) 325
Cfr13I GGNCC 1 cut(s) 59
CpoI CGGWCCG 1 cut(s) 59
CspI CGGWCCG 1 cut(s) 59
CviAII CATG 5 cut(s) 37, 168, 223, 253, 326
CviJI RGCY 6 cut(s) 67, 79, 101, 165, 184, 207
CviKI_1 RGCY 6 cut(s) 67, 79, 101, 165, 184, 207
DpnI GATC 1 cut(s) 133
DpnII GATC 1 cut(s) 131
Eco130I CCWWGG 2 cut(s) 222, 242
Eco31I GGTCTC 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 59
EcoT14I CCWWGG 2 cut(s) 222, 242
ErhI CCWWGG 2 cut(s) 222, 242
FaeI CATG 5 cut(s) 40, 171, 226, 256, 329
FaiI YATR 6 cut(s) 38, 169, 224, 254, 258, 327
FatI CATG 5 cut(s) 36, 167, 222, 252, 325
FokI GGATG 2 cut(s) 206, 249
FspBI CTAG 3 cut(s) 230, 243, 314
HapII CCGG 1 cut(s) 209
Hin1II CATG 5 cut(s) 40, 171, 226, 256, 329
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 2 cut(s) 119, 187
HpaII CCGG 1 cut(s) 209
HphI GGTGA 4 cut(s) 81, 114, 138, 147
Hpy188I TCNGA 4 cut(s) 63, 181, 236, 271
Hpy188III TCNNGA 2 cut(s) 191, 326
HpyCH4III ACNGT 3 cut(s) 30, 142, 307
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 1 cut(s) 297
HpyF10VI GCNNNNNNNGC 2 cut(s) 246, 294
HpySE526I ACGT 1 cut(s) 152
Hsp92II CATG 5 cut(s) 40, 171, 226, 256, 329
Kzo9I GATC 1 cut(s) 131
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 5 cut(s) 28, 98, 98, 176, 222
LweI GCATC 2 cut(s) 227, 252
MaeI CTAG 3 cut(s) 230, 243, 314
MaeII ACGT 1 cut(s) 152
MaeIII GTNAC 4 cut(s) 3, 87, 120, 153
MalI GATC 1 cut(s) 133
MboI GATC 1 cut(s) 131
MboII GAAGA 2 cut(s) 141, 314
MhlI GDGCHC 1 cut(s) 305
MluCI AATT 1 cut(s) 199
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 6 cut(s) 47, 90, 260, 265, 360, 364
MslI CAYNNNNRTG 2 cut(s) 221, 257
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 209
MwoI GCNNNNNNNGC 2 cut(s) 246, 294
NciI CCSGG 1 cut(s) 209
NcoI CCATGG 1 cut(s) 222
NdeII GATC 1 cut(s) 131
NlaIII CATG 5 cut(s) 40, 171, 226, 256, 329
NlaIV GGNNCC 1 cut(s) 248
NmuCI GTSAC 4 cut(s) 3, 87, 120, 153
PagI TCATGA 1 cut(s) 325
PfeI GAWTC 1 cut(s) 187
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PspN4I GGNNCC 1 cut(s) 248
PspPI GGNCC 1 cut(s) 59
PstNI CAGNNNCTG 1 cut(s) 112
RseI CAYNNNNRTG 2 cut(s) 221, 257
Rsr2I CGGWCCG 1 cut(s) 59
RsrII CGGWCCG 1 cut(s) 59
Sau3AI GATC 1 cut(s) 131
Sau96I GGNCC 1 cut(s) 59
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 209
SduI GDGCHC 1 cut(s) 305
SetI ASST 9 cut(s) 69, 75, 81, 87, 155, 167, 186, 248, 356
SfaNI GCATC 2 cut(s) 227, 252
SinI GGWCC 1 cut(s) 59
SmiMI CAYNNNNRTG 2 cut(s) 221, 257
SmlI CTYRAG 1 cut(s) 355
SmoI CTYRAG 1 cut(s) 355
Sse9I AATT 1 cut(s) 199
SsiI CCGC 2 cut(s) 57, 158
SspMI CTAG 3 cut(s) 230, 243, 314
StyD4I CCNGG 1 cut(s) 207
StyI CCWWGG 2 cut(s) 222, 242
TaaI ACNGT 3 cut(s) 30, 142, 307
TaiI ACGT 1 cut(s) 155
TasI AATT 1 cut(s) 199
TfiI GAWTC 1 cut(s) 187
TscAI CASTG 2 cut(s) 33, 310
TseFI GTSAC 4 cut(s) 3, 87, 120, 153
Tsp45I GTSAC 4 cut(s) 3, 87, 120, 153
TspDTI ATGAA 1 cut(s) 314
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 2 cut(s) 33, 310
VpaK11BI GGWCC 1 cut(s) 59
XmaJI CCTAGG 1 cut(s) 242
XspI CTAG 3 cut(s) 230, 243, 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.