MD03G1151000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010896
Physical Location & Seq
Forward (+)
16982855 .. 16983184
330 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1151000.v1.1.491

Sequence Viewer

Length: 330 bp
ATGAGCAGACGAGGAAGTAATGGTCCAAGGCTTGAATTGAGGCTGAACCTATCTCCTCCGAGGGCTAACTCATCATCACCAGTTGACTCCCCAACTGCCTCCGAGACATCCTCGGACATGTCAATCGATCACAGCTCATGCGTGTCATCGGAGCCTGATGATCAGACGATGCATTACCTGAGTAGGCCCGAAGCCAAGGCGATGGTGCTCGCAGGGTGTCCTCGGTGCCTCATGTATGTCATGCTGTCTGAAGTGGATCCAAAGTGCCCCAAATGCAAAAGCTCTGTCTTGCTCGATTTCTTTAGCGAAGGAAGCAGCAAAAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

110

Amino Acids

11.93

Weight (kDa)

7.63

Isoelectric Point (pI)

89.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn-ribbon_GIR1 PF24747 67 - 101 7.5e-23 GIR1-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 225
AclWI GGATC 2 cut(s) 251, 264
AcuI CTGAAG 1 cut(s) 270
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 1 cut(s) 35
AluBI AGCT 2 cut(s) 135, 282
AluI AGCT 2 cut(s) 135, 282
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 1 cut(s) 98
AlwI GGATC 2 cut(s) 251, 264
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 1 cut(s) 315
ArsI GACNNNNNNTTYG 2 cut(s) 270, 302
AspS9I GGNCC 2 cut(s) 23, 186
AsuHPI GGTGA 1 cut(s) 69
AvaII GGWCC 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 269
BamHI GGATCC 1 cut(s) 256
BanI GGYRCC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 210
BccI CCATC 1 cut(s) 196
BclI TGATCA 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 98
BisI GCNGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 317
Bme18I GGWCC 1 cut(s) 23
BmgT120I GGNCC 2 cut(s) 23, 186
BmiI GGNNCC 3 cut(s) 153, 227, 258
BmsI GCATC 1 cut(s) 159
BplI GAGNNNNNCTC 2 cut(s) 95, 127
Bsa29I ATCGAT 1 cut(s) 126
BsaJI CCNNGG 5 cut(s) 26, 59, 111, 195, 221
Bse1I ACTGG 1 cut(s) 80
BseCI ATCGAT 1 cut(s) 126
BseDI CCNNGG 5 cut(s) 26, 59, 111, 195, 221
BseGI GGATG 1 cut(s) 107
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 1 cut(s) 80
BseRI GAGGAG 1 cut(s) 45
BseSI GKGCMC 1 cut(s) 269
BshFI GGCC 1 cut(s) 187
BshNI GGYRCC 1 cut(s) 225
BshVI ATCGAT 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 210
BsmAI GTCTC 1 cut(s) 98
BsnI GGCC 1 cut(s) 187
Bsp1286I GDGCHC 2 cut(s) 210, 269
Bsp143I GATC 3 cut(s) 127, 160, 256
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 1 cut(s) 171
BspDI ATCGAT 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 153, 227, 258
BspPI GGATC 2 cut(s) 251, 264
BspT107I GGYRCC 1 cut(s) 225
BsrI ACTGG 1 cut(s) 80
BssECI CCNNGG 5 cut(s) 26, 59, 111, 195, 221
BssMI GATC 3 cut(s) 127, 160, 256
BssT1I CCWWGG 2 cut(s) 26, 195
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 1 cut(s) 179
BstF5I GGATG 1 cut(s) 107
BstKTI GATC 3 cut(s) 130, 163, 259
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 3 cut(s) 127, 160, 256
BstMWI GCNNNNNNNGC 2 cut(s) 273, 312
BstNSI RCATGY 1 cut(s) 121
BstSLI GKGCMC 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 256
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 1 cut(s) 256
Bsu15I ATCGAT 1 cut(s) 126
BsuRI GGCC 1 cut(s) 187
BsuTUI ATCGAT 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 215
BtsCI GGATG 1 cut(s) 107
Cac8I GCNNGC 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 23, 186
ClaI ATCGAT 1 cut(s) 126
CviAII CATG 4 cut(s) 118, 138, 232, 241
CviJI RGCY 8 cut(s) 31, 43, 65, 135, 154, 187, 194, 282
CviKI_1 RGCY 8 cut(s) 31, 43, 65, 135, 154, 187, 194, 282
DdeI CTNAG 1 cut(s) 179
DpnI GATC 3 cut(s) 129, 162, 258
DpnII GATC 3 cut(s) 127, 160, 256
Eco130I CCWWGG 2 cut(s) 26, 195
Eco47I GGWCC 1 cut(s) 23
Eco57I CTGAAG 1 cut(s) 270
EcoT14I CCWWGG 2 cut(s) 26, 195
EcoT22I ATGCAT 1 cut(s) 174
ErhI CCWWGG 2 cut(s) 26, 195
FaeI CATG 4 cut(s) 121, 141, 235, 244
FaiI YATR 5 cut(s) 119, 139, 233, 237, 242
FatI CATG 4 cut(s) 117, 137, 231, 240
FbaI TGATCA 1 cut(s) 160
Fnu4HI GCNGC 1 cut(s) 316
FokI GGATG 1 cut(s) 94
Fsp4HI GCNGC 1 cut(s) 316
GluI GCNGC 1 cut(s) 316
HaeIII GGCC 1 cut(s) 187
Hin1II CATG 4 cut(s) 121, 141, 235, 244
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 1 cut(s) 86
HphI GGTGA 1 cut(s) 69
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 6 cut(s) 60, 103, 115, 151, 165, 250
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 1 cut(s) 302
HpyCH4V TGCA 2 cut(s) 172, 276
HpyF10VI GCNNNNNNNGC 2 cut(s) 273, 312
HpyF3I CTNAG 1 cut(s) 179
Hsp92II CATG 4 cut(s) 121, 141, 235, 244
Ksp22I TGATCA 1 cut(s) 160
Kzo9I GATC 3 cut(s) 127, 160, 256
LmnI GCTCC 1 cut(s) 151
LpnPI CCDG 4 cut(s) 93, 168, 191, 198
LweI GCATC 1 cut(s) 159
MalI GATC 3 cut(s) 129, 162, 258
MboI GATC 3 cut(s) 127, 160, 256
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 2 cut(s) 210, 269
MluCI AATT 1 cut(s) 35
MlyI GAGTC 1 cut(s) 80
MnlI CCTC 8 cut(s) 5, 33, 54, 66, 109, 121, 231, 239
Mph1103I ATGCAT 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 273, 312
NdeII GATC 3 cut(s) 127, 160, 256
NlaIII CATG 4 cut(s) 121, 141, 235, 244
NlaIV GGNNCC 3 cut(s) 153, 227, 258
NsiI ATGCAT 1 cut(s) 174
NspI RCATGY 1 cut(s) 121
PciI ACATGT 1 cut(s) 117
PkrI GCNGC 1 cut(s) 317
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PscI ACATGT 1 cut(s) 117
PspN4I GGNNCC 3 cut(s) 153, 227, 258
PspPI GGNCC 2 cut(s) 23, 186
PsuI RGATCY 1 cut(s) 256
SatI GCNGC 1 cut(s) 316
Sau3AI GATC 3 cut(s) 127, 160, 256
Sau96I GGNCC 2 cut(s) 23, 186
SchI GAGTC 1 cut(s) 80
SduI GDGCHC 2 cut(s) 210, 269
SetI ASST 4 cut(s) 51, 137, 180, 284
SfaNI GCATC 1 cut(s) 159
SinI GGWCC 1 cut(s) 23
Sse9I AATT 1 cut(s) 35
StyI CCWWGG 2 cut(s) 26, 195
TaqI TCGA 2 cut(s) 126, 294
TasI AATT 1 cut(s) 35
TseI GCWGC 1 cut(s) 315
VpaK11BI GGWCC 1 cut(s) 23
XceI RCATGY 1 cut(s) 121
Zsp2I ATGCAT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.