RchiOBHm_Chr3g0475171

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0010896
Physical Location & Seq
Forward (+)
20994920 .. 20996564
1645 bp
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UTR
Exon/CDS
Intron
PRQ44068

Sequence Viewer

Length: 342 bp
ATGAGTCGCAGAAACGGAGGCGGCCCGAAGCTTGATCTGAAGCTTAACCTTTCCCCACCGAGGGCAAACCGTACGGCTGAGTCTCCCGGACGATCATCCACCGTGTCCCCCACGTCCCCGCCGAGCTCATGCGTGTCGTCGGAGCTGACTCAGGACGACAACAACAACATGAGGTACTCCAACAGCCCCGAGGCCACTTCCATGGTGCTCGTGGGTTGCCCCAGGTGTCTCATGTACGTCATGCTATCCGAGGACGACCCGAAATGCCCCAAATGCAAGAGCACTGTTCTGCTGGACTTCCTCCATGAGAACAGCAACACCATGACGACAAGGAAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

113

Amino Acids

12.32

Weight (kDa)

8.69

Isoelectric Point (pI)

67.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn-ribbon_GIR1 PF24747 66 - 102 5.6e-29 GIR1-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 21, 119
AcuI CTGAAG 1 cut(s) 59
AfaI GTAC 3 cut(s) 73, 176, 236
AfiI CCNNNNNNNGG 2 cut(s) 60, 61
AjiI CACGTC 1 cut(s) 114
AjnI CCWGG 1 cut(s) 221
AluBI AGCT 5 cut(s) 31, 43, 126, 145, 339
AluI AGCT 5 cut(s) 31, 43, 126, 145, 339
Alw21I GWGCWC 3 cut(s) 128, 210, 284
Alw26I GTCTC 2 cut(s) 87, 233
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 2 cut(s) 22, 192
AspS9I GGNCC 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 87
AvaI CYCGRG 1 cut(s) 188
BanII GRGCYC 1 cut(s) 128
BauI CACGAG 1 cut(s) 209
Bbv12I GWGCWC 3 cut(s) 128, 210, 284
BceAI ACGGC 1 cut(s) 90
BciT130I CCWGG 1 cut(s) 223
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 2 cut(s) 87, 233
BisI GCNGC 1 cut(s) 22
BlsI GCNGC 1 cut(s) 23
Bme1390I CCNGG 2 cut(s) 87, 223
BmeT110I CYCGRG 1 cut(s) 188
BmgBI CACGTC 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 23
BmrFI CCNGG 2 cut(s) 87, 223
BpuMI CCSGG 1 cut(s) 87
BsaJI CCNNGG 5 cut(s) 59, 189, 201, 221, 249
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 61
BseBI CCWGG 1 cut(s) 223
BseDI CCNNGG 5 cut(s) 59, 189, 201, 221, 249
BseGI GGATG 1 cut(s) 95
BseLI CCNNNNNNNGG 2 cut(s) 60, 61
BseMII CTCAG 2 cut(s) 69, 164
BshFI GGCC 2 cut(s) 24, 194
BsiHKAI GWGCWC 3 cut(s) 128, 210, 284
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 1 cut(s) 87
BsiWI CGTACG 1 cut(s) 71
BslFI GGGAC 2 cut(s) 91, 100
BslI CCNNNNNNNGG 2 cut(s) 60, 61
BsmAI GTCTC 2 cut(s) 87, 233
BsmFI GGGAC 2 cut(s) 91, 100
BsnI GGCC 2 cut(s) 24, 194
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 3 cut(s) 128, 210, 284
Bsp143I GATC 2 cut(s) 34, 92
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 2 cut(s) 21, 119
BspANI GGCC 2 cut(s) 24, 194
BspCNI CTCAG 2 cut(s) 70, 163
BspQI GCTCTTC 1 cut(s) 329
BssECI CCNNGG 5 cut(s) 59, 189, 201, 221, 249
BssMI GATC 2 cut(s) 34, 92
BssSI CACGAG 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 201
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 1 cut(s) 223
Bst4CI ACNGT 3 cut(s) 71, 103, 286
Bst6I CTCTTC 1 cut(s) 329
BstDEI CTNAG 2 cut(s) 78, 150
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 95
BstKTI GATC 2 cut(s) 37, 95
BstMAI GTCTC 2 cut(s) 87, 233
BstMBI GATC 2 cut(s) 34, 92
BstMWI GCNNNNNNNGC 1 cut(s) 273
BstNI CCWGG 1 cut(s) 223
BstSCI CCNGG 2 cut(s) 85, 221
BstXI CCANNNNNNTGG 1 cut(s) 202
BsuRI GGCC 2 cut(s) 24, 194
BtgI CCRYGG 1 cut(s) 201
BtrI CACGTC 1 cut(s) 114
BtsCI GGATG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 282
Cfr13I GGNCC 1 cut(s) 23
Csp6I GTAC 3 cut(s) 72, 175, 235
CviAII CATG 7 cut(s) 129, 169, 202, 232, 241, 305, 322
CviJI RGCY 9 cut(s) 24, 31, 43, 77, 126, 145, 186, 194, 339
CviKI_1 RGCY 9 cut(s) 24, 31, 43, 77, 126, 145, 186, 194, 339
CviQI GTAC 3 cut(s) 72, 175, 235
DdeI CTNAG 2 cut(s) 78, 150
DpnI GATC 2 cut(s) 36, 94
DpnII GATC 2 cut(s) 34, 92
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Ecl136II GAGCTC 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 128
Eco53kI GAGCTC 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 126
EcoRII CCWGG 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 7 cut(s) 132, 172, 205, 235, 244, 308, 325
FaiI YATR 7 cut(s) 130, 170, 203, 233, 242, 306, 323
FaqI GGGAC 2 cut(s) 91, 100
FatI CATG 7 cut(s) 128, 168, 201, 231, 240, 304, 321
FauI CCCGC 1 cut(s) 126
Fnu4HI GCNGC 1 cut(s) 22
FokI GGATG 1 cut(s) 82
FriOI GRGCYC 1 cut(s) 128
Fsp4HI GCNGC 1 cut(s) 22
GluI GCNGC 1 cut(s) 22
HaeIII GGCC 2 cut(s) 24, 194
HapII CCGG 1 cut(s) 87
Hin1II CATG 7 cut(s) 132, 172, 205, 235, 244, 308, 325
HindIII AAGCTT 2 cut(s) 29, 41
HinfI GANTC 3 cut(s) 4, 80, 148
HpaII CCGG 1 cut(s) 87
Hpy188I TCNGA 3 cut(s) 39, 142, 250
Hpy188III TCNNGA 1 cut(s) 152
Hpy99I CGWCG 1 cut(s) 142
HpyCH4III ACNGT 3 cut(s) 71, 103, 286
HpyCH4IV ACGT 2 cut(s) 113, 237
HpyCH4V TGCA 1 cut(s) 276
HpyF10VI GCNNNNNNNGC 1 cut(s) 273
HpyF3I CTNAG 2 cut(s) 78, 150
HpySE526I ACGT 2 cut(s) 113, 237
Hsp92II CATG 7 cut(s) 132, 172, 205, 235, 244, 308, 325
Kzo9I GATC 2 cut(s) 34, 92
LguI GCTCTTC 1 cut(s) 329
LmnI GCTCC 1 cut(s) 142
LpnPI CCDG 5 cut(s) 100, 137, 208, 235, 278
MaeII ACGT 2 cut(s) 113, 237
MalI GATC 2 cut(s) 36, 94
MboI GATC 2 cut(s) 34, 92
MhlI GDGCHC 3 cut(s) 128, 210, 284
MlyI GAGTC 3 cut(s) 13, 89, 142
MmeI TCCRAC 2 cut(s) 120, 204
MnlI CCTC 6 cut(s) 11, 54, 165, 184, 244, 311
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 1 cut(s) 200
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 2 cut(s) 87, 223
MvaI CCWGG 1 cut(s) 223
MwoI GCNNNNNNNGC 1 cut(s) 273
NciI CCSGG 1 cut(s) 87
NcoI CCATGG 1 cut(s) 201
NdeII GATC 2 cut(s) 34, 92
NlaIII CATG 7 cut(s) 132, 172, 205, 235, 244, 308, 325
NmeAIII GCCGAG 1 cut(s) 147
PciSI GCTCTTC 1 cut(s) 329
PcsI WCGNNNNNNNCGW 1 cut(s) 119
Pfl23II CGTACG 1 cut(s) 71
PfoI TCCNGGA 1 cut(s) 85
PkrI GCNGC 1 cut(s) 23
PleI GAGTC 3 cut(s) 12, 88, 142
PpsI GAGTC 3 cut(s) 12, 88, 142
Psp124BI GAGCTC 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 221
PspGI CCWGG 1 cut(s) 221
PspLI CGTACG 1 cut(s) 71
PspPI GGNCC 1 cut(s) 23
RsaI GTAC 3 cut(s) 73, 176, 236
RsaNI GTAC 3 cut(s) 72, 175, 235
RseI CAYNNNNRTG 1 cut(s) 200
SacI GAGCTC 1 cut(s) 128
SapI GCTCTTC 1 cut(s) 329
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 1 cut(s) 22
Sau3AI GATC 2 cut(s) 34, 92
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 3 cut(s) 13, 89, 142
ScrFI CCNGG 2 cut(s) 87, 223
SduI GDGCHC 3 cut(s) 128, 210, 284
SmiMI CAYNNNNRTG 1 cut(s) 200
SsiI CCGC 2 cut(s) 21, 119
SstI GAGCTC 1 cut(s) 128
StyD4I CCNGG 2 cut(s) 85, 221
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 3 cut(s) 71, 103, 286
TaiI ACGT 2 cut(s) 116, 240
TauI GCSGC 1 cut(s) 24
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 1 cut(s) 289
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.