pycom11g14430

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010896
Physical Location & Seq
Forward (+)
14388247 .. 14388579
333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g14430.1

Sequence Viewer

Length: 333 bp
ATGAGCAGAAGAGGAAGTAGTAGTCCAAAGCTTGAATTGAAGTTAAATCTGTCTCCTCCAAAGGCTAACTCCTCACTAATTCACTCCCCAAATGCCTCCGAGTCATCCATGGACATGTCAATCGACCGCAGCTCATGCGTGTCGTCGGAACCTGATGACGAGACGATTCATTGCCTGAGCAGGCCCGAAGCCAACGTGATGGTGCTCGCAGGGTGTCCTCGGTGCCTCATGTATGTCATGCTATCTGAATTGGATCCAAAGTGCCCAAAATGCAAAAGCTCTGTCTTGCTCGATTTCCTTAATGCAGAAAGCACCAAGAGGAAGGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

111

Amino Acids

12.0

Weight (kDa)

8.24

Isoelectric Point (pI)

79.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn-ribbon_GIR1 PF24747 67 - 100 3e-23 GIR1-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 222
AciI CCGC 1 cut(s) 127
AclWI GGATC 2 cut(s) 248, 261
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 2 cut(s) 35, 40
AluBI AGCT 3 cut(s) 31, 132, 279
AluI AGCT 3 cut(s) 31, 132, 279
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 2 cut(s) 57, 155
AlwI GGATC 2 cut(s) 248, 261
AoxI GGCC 1 cut(s) 182
ApeKI GCWGC 1 cut(s) 129
ArsI GACNNNNNNTTYG 2 cut(s) 267, 299
AspS9I GGNCC 1 cut(s) 183
BaeGI GKGCMC 1 cut(s) 266
BamHI GGATCC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 141
BccI CCATC 1 cut(s) 193
BcoDI GTCTC 2 cut(s) 57, 155
BisI GCNGC 1 cut(s) 130
BlsI GCNGC 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 183
BmiI GGNNCC 3 cut(s) 150, 224, 255
Bpu10I CCTNAGC 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 108, 218
Bse3DI GCAATG 1 cut(s) 169
BseDI CCNNGG 2 cut(s) 108, 218
BseGI GGATG 1 cut(s) 104
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 1 cut(s) 167
BseRI GAGGAG 2 cut(s) 45, 61
BseSI GKGCMC 1 cut(s) 266
BseXI GCAGC 1 cut(s) 141
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 1 cut(s) 184
BshNI GGYRCC 1 cut(s) 222
BsiEI CGRYCG 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 207
BsmAI GTCTC 2 cut(s) 57, 155
BsmBI CGTCTC 1 cut(s) 155
BsnI GGCC 1 cut(s) 184
Bsp1286I GDGCHC 2 cut(s) 207, 266
Bsp143I GATC 1 cut(s) 253
Bsp19I CCATGG 1 cut(s) 108
BspACI CCGC 1 cut(s) 127
BspANI GGCC 1 cut(s) 184
BspCNI CTCAG 1 cut(s) 168
BspLI GGNNCC 3 cut(s) 150, 224, 255
BspPI GGATC 2 cut(s) 248, 261
BspT107I GGYRCC 1 cut(s) 222
BsrDI GCAATG 1 cut(s) 169
BssECI CCNNGG 2 cut(s) 108, 218
BssMI GATC 1 cut(s) 253
BssT1I CCWWGG 1 cut(s) 108
Bst6I CTCTTC 1 cut(s) 4
BstAPI GCANNNNNTGC 1 cut(s) 135
BstC8I GCNNGC 2 cut(s) 182, 207
BstDEI CTNAG 1 cut(s) 176
BstDSI CCRYGG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 104
BstKTI GATC 1 cut(s) 256
BstMAI GTCTC 2 cut(s) 57, 155
BstMBI GATC 1 cut(s) 253
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 2 cut(s) 135, 270
BstNSI RCATGY 1 cut(s) 118
BstSLI GKGCMC 1 cut(s) 266
BstV1I GCAGC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 253
BstXI CCANNNNNNTGG 1 cut(s) 199
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 1 cut(s) 184
BtgI CCRYGG 1 cut(s) 108
BtsCI GGATG 1 cut(s) 104
Cac8I GCNNGC 2 cut(s) 182, 207
Cfr13I GGNCC 1 cut(s) 183
CviAII CATG 5 cut(s) 109, 115, 135, 229, 238
CviJI RGCY 6 cut(s) 31, 65, 132, 184, 191, 279
CviKI_1 RGCY 6 cut(s) 31, 65, 132, 184, 191, 279
DdeI CTNAG 1 cut(s) 176
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 4
EarI CTCTTC 1 cut(s) 4
Eco130I CCWWGG 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 108
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 5 cut(s) 112, 118, 138, 232, 241
FaiI YATR 6 cut(s) 110, 116, 136, 230, 234, 239
FatI CATG 5 cut(s) 108, 114, 134, 228, 237
Fnu4HI GCNGC 1 cut(s) 130
FokI GGATG 1 cut(s) 91
Fsp4HI GCNGC 1 cut(s) 130
GluI GCNGC 1 cut(s) 130
HaeIII GGCC 1 cut(s) 184
Hin1II CATG 5 cut(s) 112, 118, 138, 232, 241
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 2 cut(s) 101, 166
Hpy188I TCNGA 3 cut(s) 100, 148, 247
Hpy99I CGWCG 1 cut(s) 148
HpyAV CCTTC 1 cut(s) 316
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 2 cut(s) 273, 305
HpyF10VI GCNNNNNNNGC 2 cut(s) 135, 270
HpyF3I CTNAG 1 cut(s) 176
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 5 cut(s) 112, 118, 138, 232, 241
Kzo9I GATC 1 cut(s) 253
LpnPI CCDG 4 cut(s) 165, 166, 188, 195
Lsp1109I GCAGC 1 cut(s) 141
MaeII ACGT 1 cut(s) 195
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MboII GAAGA 1 cut(s) 21
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 2 cut(s) 207, 266
MluCI AATT 3 cut(s) 35, 78, 248
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 7 cut(s) 5, 66, 82, 106, 228, 236, 312
MseI TTAA 2 cut(s) 44, 300
MslI CAYNNNNRTG 1 cut(s) 113
MwoI GCNNNNNNNGC 2 cut(s) 135, 270
NcoI CCATGG 1 cut(s) 108
NdeII GATC 1 cut(s) 253
NlaIII CATG 5 cut(s) 112, 118, 138, 232, 241
NlaIV GGNNCC 3 cut(s) 150, 224, 255
NspI RCATGY 1 cut(s) 118
PciI ACATGT 1 cut(s) 114
PfeI GAWTC 1 cut(s) 166
PkrI GCNGC 1 cut(s) 131
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PscI ACATGT 1 cut(s) 114
PspN4I GGNNCC 3 cut(s) 150, 224, 255
PspPI GGNCC 1 cut(s) 183
PsuI RGATCY 1 cut(s) 253
RseI CAYNNNNRTG 1 cut(s) 113
SaqAI TTAA 2 cut(s) 44, 300
SatI GCNGC 1 cut(s) 130
Sau3AI GATC 1 cut(s) 253
Sau96I GGNCC 1 cut(s) 183
SchI GAGTC 1 cut(s) 110
SduI GDGCHC 2 cut(s) 207, 266
SetI ASST 5 cut(s) 33, 134, 154, 198, 281
SmiMI CAYNNNNRTG 1 cut(s) 113
Sse9I AATT 3 cut(s) 35, 78, 248
SsiI CCGC 1 cut(s) 127
StyI CCWWGG 1 cut(s) 108
TaiI ACGT 1 cut(s) 198
TaqI TCGA 2 cut(s) 123, 291
TasI AATT 3 cut(s) 35, 78, 248
TfiI GAWTC 1 cut(s) 166
Tru1I TTAA 2 cut(s) 44, 300
Tru9I TTAA 2 cut(s) 44, 300
TseI GCWGC 1 cut(s) 129
TspDTI ATGAA 1 cut(s) 158
XceI RCATGY 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.