AT5G10120

Ethylene insensitive 3 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009800
Physical Location & Seq
Forward (+)
3169640 .. 3171262
1623 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10120.1

Sequence Viewer

Length: 1416 bp
ATGGTGGAAGTCGAAGAATTGGAACCACTTAGTCCAATGGACGATGAAGAAGAAGAGATAAGCTATGATGACCTCAAAAGACGCATGTGGAAAGACCGAAACCTCATGGAAAAAAAGCTCAAGCAACAGAAACGACACAGCAACGACGTCGTTTCGTTTACAACACACCGAGCCGAAGCTTCACGGCGCAAGAAGATGGCTCGTTCTCAAGATTCAGTGTTGAAGTACATGATGAAGATCATGGAGGTGTGTAAAGCTAAAGGTTTTGTATACGGAATTGTACCGGAAAAGGGTAAACCAATAACCGGTTCTTCCGATAGCTTAAGACGTTGGTGGAAAGAAAACGTTCAGTTCGACCAAAACGCTCCAGACGCTATCACAGATTACTTAGCACTCGCGGCTGCTGCTGCTGCAGCAGAGCTAATCGATAAGTCCTCATCATCCTCGAGCTTATTACACATGTTACAGGAACTGCAAGACACGACACTAGGGTCGTTACTATCAGCGTTAATGCAACATTGCATGCCGCCACAGCGGCGGTTTCCGTTAGAGAAAGGAATTGCTCCGCCATGGTGGCCGACGGGGACGGAGCTTTGGTGGGGAGAACAAGGAGCGGCACATGAGCACGGTGCACCGCCATATCGAAAGCCGCACGATTTGAGAAAATCTTGGAAAGTTAGTGTTCTCGCGGCTGTGATTAAACACATGTCGCCGAATTTGGGAAGAGTGAGACGTCTGGCGAGACAATCTAAAAGTTTGCAAGATAAAATGATGGCTAAAGAGACTGATACTTGGTCTCGTGTCTTAAACCAAGAAGAAGCTCTTCTCAATATCAAAGACCTCAAGATCTCGGAAGATCAGGACGATCAAGAATCGTCGGGGTCTAAGAGAAAAAGTGAGTCTATGGAACCGTCTAAGAGTGTTTACACTTGCCAAAACTCGAGTTGTCCTAAAAGTGATGTGAGTTTTGGATTCGGGGACAAGAACTCGAGGACGGGTCATGAGATCCAGTGTCTTTATGGGTCGAACCAAGAACCGAGCCAGAGCGGTGAATATACATCTTCCTTGCTTGAAACGGTTCCAAGTATTGTTACTAATTCGACTAGTGAAGATGATTATTACAATGTGAGCTCGCGTGCACTGGACAAGCGAGATGATGATGATCATAGCATTAATGGGAATTGGATGGAATATTTCTGGTTAGAAAAGATGCAGCAGGAGTTTCATTGCTCTAGGAGATTCGAAGATGATGAGGGTACTGGAACGGACTTTGACCAACTCACAGAATCTGATCGATCGGACAACGTGAATTTGAACCAACTTACAAAATCTGATCGATCGGACAACGTGAACCGGAGTGCTTTTTCAGTTTGGGACATGGGTTGCGAGGACAAAGACATATACATGTTCGATTAA

Protein Analysis

471

Amino Acids

53.95

Weight (kDa)

5.31

Isoelectric Point (pI)

61.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIN3_DNA-bd PF04873 23 - 273 5.8e-116 Ethylene insensitive 3, DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 490
AatII GACGTC 2 cut(s) 150, 736
AccBSI CCGCTC 2 cut(s) 614, 1047
AccI GTMKAC 1 cut(s) 270
AccII CGCG 3 cut(s) 398, 689, 1135
AclI AACGTT 1 cut(s) 345
AclWI GGATC 1 cut(s) 1000
AcoI YGGCCR 1 cut(s) 575
AcsI RAATTY 2 cut(s) 715, 1309
AcyI GRCGYC 2 cut(s) 147, 733
AfaI GTAC 3 cut(s) 227, 282, 1258
AfiI CCNNNNNNNGG 3 cut(s) 290, 305, 719
AflII CTTAAG 1 cut(s) 322
AflIII ACRYGT 3 cut(s) 459, 705, 1404
AgeI ACCGGT 1 cut(s) 305
AgsI TTSAA 3 cut(s) 223, 1073, 1315
AhlI ACTAGT 1 cut(s) 1103
AjuI GAANNNNNNNTTGG 2 cut(s) 292, 324
Alw21I GWGCWC 4 cut(s) 627, 634, 1133, 1141
Alw26I GTCTC 4 cut(s) 724, 736, 776, 801
Alw44I GTGCAC 2 cut(s) 630, 1137
AlwI GGATC 1 cut(s) 1000
AlwNI CAGNNNCTG 2 cut(s) 472, 1289
Ama87I CYCGRG 3 cut(s) 445, 940, 988
AoxI GGCC 1 cut(s) 575
ApaLI GTGCAC 2 cut(s) 630, 1137
ApeKI GCWGC 6 cut(s) 401, 404, 407, 410, 413, 1213
ApoI RAATTY 2 cut(s) 715, 1309
AseI ATTAAT 1 cut(s) 1173
AsiGI ACCGGT 1 cut(s) 305
Asp700I GAANNNNTTC 3 cut(s) 345, 822, 1077
AspLEI GCGC 1 cut(s) 189
AsuHPI GGTGA 1 cut(s) 1061
AsuII TTCGAA 1 cut(s) 1242
AvaI CYCGRG 3 cut(s) 445, 940, 988
BaeGI GKGCMC 2 cut(s) 634, 1141
BanII GRGCYC 1 cut(s) 1133
BauI CACGAG 1 cut(s) 798
Bbv12I GWGCWC 4 cut(s) 627, 634, 1133, 1141
BbvI GCAGC 6 cut(s) 388, 391, 394, 397, 425, 1225
BccI CCATC 3 cut(s) 190, 766, 1180
BceAI ACGGC 1 cut(s) 200
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BclI TGATCA 1 cut(s) 1162
BcoDI GTCTC 4 cut(s) 724, 736, 776, 801
BcuI ACTAGT 1 cut(s) 1103
BfaI CTAG 3 cut(s) 488, 1104, 1233
BfmI CTRYAG 1 cut(s) 411
BfrI CTTAAG 1 cut(s) 322
BglI GCCNNNNNGGC 2 cut(s) 535, 574
BglII AGATCT 1 cut(s) 846
BmeT110I CYCGRG 3 cut(s) 445, 940, 988
BmiI GGNNCC 3 cut(s) 24, 909, 1080
BmsI GCATC 1 cut(s) 1200
BpmI CTGGAG 1 cut(s) 351
Bpu14I TTCGAA 1 cut(s) 1242
BpuEI CTTGAG 3 cut(s) 104, 192, 827
Bsa29I ATCGAT 3 cut(s) 426, 1294, 1336
BsaBI GATNNNNATC 2 cut(s) 236, 1161
BsaHI GRCGYC 2 cut(s) 147, 733
BsaI GGTCTC 1 cut(s) 801
BsaJI CCNNGG 1 cut(s) 569
BsaWI WCCGGW 3 cut(s) 283, 305, 1353
BsaXI ACNNNNNCTCC 2 cut(s) 581, 611
Bsc4I CCNNNNNNNGG 3 cut(s) 290, 305, 719
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 3 cut(s) 1009, 1146, 1264
Bse3DI GCAATG 2 cut(s) 517, 1225
Bse8I GATNNNNATC 2 cut(s) 236, 1161
BseCI ATCGAT 3 cut(s) 426, 1294, 1336
BseDI CCNNGG 1 cut(s) 569
BseGI GGATG 2 cut(s) 440, 1191
BseJI GATNNNNATC 2 cut(s) 236, 1161
BseLI CCNNNNNNNGG 3 cut(s) 290, 305, 719
BseMI GCAATG 2 cut(s) 517, 1225
BseNI ACTGG 3 cut(s) 1009, 1146, 1264
BseSI GKGCMC 2 cut(s) 634, 1141
BseXI GCAGC 6 cut(s) 388, 391, 394, 397, 425, 1225
Bsh1236I CGCG 3 cut(s) 398, 689, 1135
Bsh1285I CGRYCG 2 cut(s) 1298, 1340
BshFI GGCC 1 cut(s) 577
BshTI ACCGGT 1 cut(s) 305
BshVI ATCGAT 3 cut(s) 426, 1294, 1336
BsiEI CGRYCG 2 cut(s) 1298, 1340
BsiHKAI GWGCWC 4 cut(s) 627, 634, 1133, 1141
BsiHKCI CYCGRG 3 cut(s) 445, 940, 988
BsiSI CCGG 3 cut(s) 284, 306, 1354
BslFI GGGAC 3 cut(s) 598, 992, 1388
BslI CCNNNNNNNGG 3 cut(s) 290, 305, 719
BsmAI GTCTC 4 cut(s) 724, 736, 776, 801
BsmBI CGTCTC 1 cut(s) 724
BsmFI GGGAC 3 cut(s) 598, 992, 1388
BsnI GGCC 1 cut(s) 577
Bso31I GGTCTC 1 cut(s) 801
BsoBI CYCGRG 3 cut(s) 445, 940, 988
Bsp119I TTCGAA 1 cut(s) 1242
Bsp1286I GDGCHC 4 cut(s) 627, 634, 1133, 1141
Bsp19I CCATGG 1 cut(s) 569
BspANI GGCC 1 cut(s) 577
BspDI ATCGAT 3 cut(s) 426, 1294, 1336
BspFNI CGCG 3 cut(s) 398, 689, 1135
BspHI TCATGA 1 cut(s) 1000
BspLI GGNNCC 3 cut(s) 24, 909, 1080
BspMAI CTGCAG 1 cut(s) 415
BspPI GGATC 1 cut(s) 1000
BspQI GCTCTTC 1 cut(s) 828
BspT104I TTCGAA 1 cut(s) 1242
BspTI CTTAAG 1 cut(s) 322
BspTNI GGTCTC 1 cut(s) 801
BsrBI CCGCTC 2 cut(s) 614, 1047
BsrDI GCAATG 2 cut(s) 517, 1225
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 3 cut(s) 1009, 1146, 1264
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 1 cut(s) 569
BssNAI GTATAC 1 cut(s) 271
BssNI GRCGYC 2 cut(s) 147, 733
BssSI CACGAG 1 cut(s) 798
BssT1I CCWWGG 1 cut(s) 569
Bst1107I GTATAC 1 cut(s) 271
Bst2BI CACGAG 1 cut(s) 798
Bst4CI ACNGT 3 cut(s) 629, 912, 1078
Bst6I CTCTTC 3 cut(s) 48, 718, 828
BstACI GRCGYC 2 cut(s) 147, 733
BstAFI CTTAAG 1 cut(s) 322
BstBI TTCGAA 1 cut(s) 1242
BstC8I GCNNGC 3 cut(s) 524, 1133, 1137
BstDEI CTNAG 4 cut(s) 29, 388, 885, 915
BstDSI CCRYGG 1 cut(s) 569
BstF5I GGATG 2 cut(s) 440, 1191
BstFNI CGCG 3 cut(s) 398, 689, 1135
BstHHI GCGC 1 cut(s) 189
BstMAI GTCTC 4 cut(s) 724, 736, 776, 801
BstMCI CGRYCG 2 cut(s) 1298, 1340
BstMWI GCNNNNNNNGC 9 cut(s) 371, 398, 404, 407, 410, 413, 532, 535, 574
BstNSI RCATGY 5 cut(s) 88, 463, 526, 709, 1408
BstSFI CTRYAG 1 cut(s) 411
BstSLI GKGCMC 2 cut(s) 634, 1141
BstUI CGCG 3 cut(s) 398, 689, 1135
BstV1I GCAGC 6 cut(s) 388, 391, 394, 397, 425, 1225
BstX2I RGATCY 2 cut(s) 846, 1005
BstYI RGATCY 2 cut(s) 846, 1005
BstZ17I GTATAC 1 cut(s) 271
Bsu15I ATCGAT 3 cut(s) 426, 1294, 1336
BsuRI GGCC 1 cut(s) 577
BsuTUI ATCGAT 3 cut(s) 426, 1294, 1336
BtgI CCRYGG 1 cut(s) 569
BtsCI GGATG 2 cut(s) 440, 1191
BtsIMutI CAGTG 3 cut(s) 222, 1016, 1139
Cac8I GCNNGC 3 cut(s) 524, 1133, 1137
CaiI CAGNNNCTG 2 cut(s) 472, 1289
CciI TCATGA 1 cut(s) 1000
CfoI GCGC 1 cut(s) 189
Cfr10I RCCGGY 1 cut(s) 305
ClaI ATCGAT 3 cut(s) 426, 1294, 1336
CseI GACGC 2 cut(s) 90, 380
Csp6I GTAC 3 cut(s) 226, 281, 1257
CspAI ACCGGT 1 cut(s) 305
CviQI GTAC 3 cut(s) 226, 281, 1257
DdeI CTNAG 4 cut(s) 29, 388, 885, 915
DrdI GACNNNNNNGTC 1 cut(s) 490
DseDI GACNNNNNNGTC 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 575
Eam1104I CTCTTC 3 cut(s) 48, 718, 828
EarI CTCTTC 3 cut(s) 48, 718, 828
EciI GGCGGA 1 cut(s) 555
Ecl136II GAGCTC 1 cut(s) 1131
Eco130I CCWWGG 1 cut(s) 569
Eco24I GRGCYC 1 cut(s) 1133
Eco31I GGTCTC 1 cut(s) 801
Eco53kI GAGCTC 1 cut(s) 1131
Eco88I CYCGRG 3 cut(s) 445, 940, 988
EcoICRI GAGCTC 1 cut(s) 1131
EcoT14I CCWWGG 1 cut(s) 569
EcoT38I GRGCYC 1 cut(s) 1133
ErhI CCWWGG 1 cut(s) 569
Esp3I CGTCTC 1 cut(s) 724
FalI AAGNNNNNCTT 2 cut(s) 807, 839
FaqI GGGAC 3 cut(s) 598, 992, 1388
FbaI TGATCA 1 cut(s) 1162
FblI GTMKAC 1 cut(s) 270
FokI GGATG 2 cut(s) 427, 1198
FriOI GRGCYC 1 cut(s) 1133
FspBI CTAG 3 cut(s) 488, 1104, 1233
GlaI GCGC 1 cut(s) 188
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 1 cut(s) 577
HapII CCGG 3 cut(s) 284, 306, 1354
HgaI GACGC 2 cut(s) 90, 380
HhaI GCGC 1 cut(s) 189
Hin1I GRCGYC 2 cut(s) 147, 733
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HindIII AAGCTT 1 cut(s) 177
HinfI GANTC 6 cut(s) 212, 872, 899, 972, 1239, 1286
HpaII CCGG 3 cut(s) 284, 306, 1354
HphI GGTGA 1 cut(s) 1061
Hpy166II GTNNAC 7 cut(s) 159, 271, 296, 632, 925, 1139, 1351
Hpy188I TCNGA 6 cut(s) 316, 853, 1291, 1300, 1333, 1342
Hpy188III TCNNGA 6 cut(s) 209, 368, 844, 860, 869, 1001
Hpy8I GTNNAC 7 cut(s) 159, 271, 296, 632, 925, 1139, 1351
Hpy99I CGWCG 4 cut(s) 149, 152, 583, 880
HpyCH4III ACNGT 3 cut(s) 629, 912, 1078
HpyCH4IV ACGT 6 cut(s) 147, 328, 345, 733, 1305, 1347
HpyCH4V TGCA 8 cut(s) 413, 475, 514, 522, 632, 760, 1139, 1213
HpyF10VI GCNNNNNNNGC 9 cut(s) 371, 398, 404, 407, 410, 413, 532, 535, 574
HpyF3I CTNAG 4 cut(s) 29, 388, 885, 915
HpySE526I ACGT 6 cut(s) 147, 328, 345, 733, 1305, 1347
Hsp92I GRCGYC 2 cut(s) 147, 733
HspAI GCGC 1 cut(s) 187
Ksp22I TGATCA 1 cut(s) 1162
LguI GCTCTTC 1 cut(s) 828
LmnI GCTCC 4 cut(s) 370, 568, 589, 611
Lsp1109I GCAGC 6 cut(s) 388, 391, 394, 397, 425, 1225
LweI GCATC 1 cut(s) 1200
MaeI CTAG 3 cut(s) 488, 1104, 1233
MaeII ACGT 6 cut(s) 147, 328, 345, 733, 1305, 1347
MaeIII GTNAC 3 cut(s) 462, 495, 1090
MbiI CCGCTC 2 cut(s) 614, 1047
MflI RGATCY 2 cut(s) 846, 1005
MhlI GDGCHC 4 cut(s) 627, 634, 1133, 1141
MluCI AATT 7 cut(s) 17, 276, 558, 715, 1096, 1180, 1309
MlyI GAGTC 1 cut(s) 908
MnlI CCTC 9 cut(s) 83, 113, 238, 445, 454, 851, 984, 1246, 1381
MroXI GAANNNNTTC 3 cut(s) 345, 822, 1077
MseI TTAA 6 cut(s) 323, 509, 699, 806, 1173, 1414
MslI CAYNNNNRTG 2 cut(s) 245, 1403
MspA1I CMGCKG 1 cut(s) 535
MspCI CTTAAG 1 cut(s) 322
MspI CCGG 3 cut(s) 284, 306, 1354
MvnI CGCG 3 cut(s) 398, 689, 1135
MwoI GCNNNNNNNGC 9 cut(s) 371, 398, 404, 407, 410, 413, 532, 535, 574
NcoI CCATGG 1 cut(s) 569
NlaIV GGNNCC 3 cut(s) 24, 909, 1080
NspI RCATGY 5 cut(s) 88, 463, 526, 709, 1408
NspV TTCGAA 1 cut(s) 1242
PaeI GCATGC 1 cut(s) 526
PaeR7I CTCGAG 3 cut(s) 445, 940, 988
PagI TCATGA 1 cut(s) 1000
PciI ACATGT 3 cut(s) 459, 705, 1404
PciSI GCTCTTC 1 cut(s) 828
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PdmI GAANNNNTTC 3 cut(s) 345, 822, 1077
PfeI GAWTC 5 cut(s) 212, 872, 972, 1239, 1286
PinAI ACCGGT 1 cut(s) 305
Ple19I CGATCG 2 cut(s) 1298, 1340
PleI GAGTC 1 cut(s) 907
PpsI GAGTC 1 cut(s) 907
PscI ACATGT 3 cut(s) 459, 705, 1404
PshBI ATTAAT 1 cut(s) 1173
Psp124BI GAGCTC 1 cut(s) 1133
Psp1406I AACGTT 1 cut(s) 345
PspN4I GGNNCC 3 cut(s) 24, 909, 1080
PspXI VCTCGAGB 3 cut(s) 445, 940, 988
PstI CTGCAG 1 cut(s) 415
PstNI CAGNNNCTG 2 cut(s) 472, 1289
PsuI RGATCY 2 cut(s) 846, 1005
PvuI CGATCG 2 cut(s) 1298, 1340
RsaI GTAC 3 cut(s) 227, 282, 1258
RsaNI GTAC 3 cut(s) 226, 281, 1257
RseI CAYNNNNRTG 2 cut(s) 245, 1403
SacI GAGCTC 1 cut(s) 1133
SapI GCTCTTC 1 cut(s) 828
SaqAI TTAA 6 cut(s) 323, 509, 699, 806, 1173, 1414
SchI GAGTC 1 cut(s) 908
SduI GDGCHC 4 cut(s) 627, 634, 1133, 1141
SfaNI GCATC 1 cut(s) 1200
SfcI CTRYAG 1 cut(s) 411
Sfr274I CTCGAG 3 cut(s) 445, 940, 988
SfuI TTCGAA 1 cut(s) 1242
SlaI CTCGAG 3 cut(s) 445, 940, 988
SmiMI CAYNNNNRTG 2 cut(s) 245, 1403
SmlI CTYRAG 7 cut(s) 119, 207, 322, 445, 842, 940, 988
SmoI CTYRAG 7 cut(s) 119, 207, 322, 445, 842, 940, 988
SpeI ACTAGT 1 cut(s) 1103
SphI GCATGC 1 cut(s) 526
Sse9I AATT 7 cut(s) 17, 276, 558, 715, 1096, 1180, 1309
SspI AATATT 1 cut(s) 1193
SspMI CTAG 3 cut(s) 488, 1104, 1233
SstI GAGCTC 1 cut(s) 1133
StyI CCWWGG 1 cut(s) 569
TaaI ACNGT 3 cut(s) 629, 912, 1078
TaiI ACGT 6 cut(s) 150, 331, 348, 736, 1308, 1350
TaqII GACCGA 1 cut(s) 111
TasI AATT 7 cut(s) 17, 276, 558, 715, 1096, 1180, 1309
TatI WGTACW 1 cut(s) 225
TauI GCSGC 6 cut(s) 401, 529, 538, 617, 652, 692
TfiI GAWTC 5 cut(s) 212, 872, 972, 1239, 1286
Tru1I TTAA 6 cut(s) 323, 509, 699, 806, 1173, 1414
Tru9I TTAA 6 cut(s) 323, 509, 699, 806, 1173, 1414
TscAI CASTG 3 cut(s) 222, 1016, 1146
TseI GCWGC 6 cut(s) 401, 404, 407, 410, 413, 1213
TspDTI ATGAA 3 cut(s) 60, 248, 1214
TspGWI ACGGA 4 cut(s) 288, 534, 602, 1280
TspRI CASTG 3 cut(s) 222, 1016, 1146
Vha464I CTTAAG 1 cut(s) 322
VneI GTGCAC 2 cut(s) 630, 1137
VspI ATTAAT 1 cut(s) 1173
XapI RAATTY 2 cut(s) 715, 1309
XceI RCATGY 5 cut(s) 88, 463, 526, 709, 1408
XcmI CCANNNNNNNNNTGG 1 cut(s) 1016
XhoI CTCGAG 3 cut(s) 445, 940, 988
XmiI GTMKAC 1 cut(s) 270
XmnI GAANNNNTTC 3 cut(s) 345, 822, 1077
XspI CTAG 3 cut(s) 488, 1104, 1233
ZraI GACGTC 2 cut(s) 148, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.