Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009800

Physical Location & Seq

Genomic Coordinates Forward (+)

35385114 .. 35386238

Gene Structure 1125 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1327300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1125 bp

ATGGTAGAGTTCCATGGAGAAGTTGATACTGTAAGGGTTGGGGTAGTTGTAGAAGATGAGGAAGCATCTGAAGATCACATCAGCTACGAAGAGCTCAAGAAGCGAATGTGGAAGGATCGTATTTGCTTACAAAATCTCAAAGAGAGTGGAAAAATCATTGAGGACGAAGAAGACCGTGAAGATGAAGAAGAACCTGCAGAGTCGTTGGCAAAGCAAGAAGCGTCTCGTCGAAAGAAAATGGCAAGATCCCAAGATTCGATACTGAAATACATGGTGAAAATCATGGAGATATGCAAAGCAAAGGGCTTTGTGTATGGGATTGTGCCTGAGAAGGGAAAGCCAATGACGGGCTCCTCTTATAGCTTAAGAGAGTGGTGGAAAGACAAAGTGAGATTTGACCAAAACGCCCCCATTGCAATATCCGAGCATCTGCCAGCTTTGATTTTTGAACAAGGTGAGTTGGATCATGGTTCTTATGCTCATTTGCTTCATGAACTGCAAGACACTACTTTGGGGTTTCTTCTTTCTGCTCTGATGCAGCACTGCATGCCGCCACAGCGGAGGTTCCCTCTGGAGAAGGGTTTGGCTCCCCCTTGGTGGCCCACGGGGGAGGAGATGTGGTGGGGGGACCAAGGGCTTTCTGTAGAGCATGTCGCTCCTCCTTATCTTAAAAAGGCATGGAATGTAAATGCCTTGGCTGCTGTGATCAAACACATGGCCCCTAATTCGGATAAAGTGAGAAAGCTTGTGAGGCAAAGCAAGTGTTTGCAAGATAAGATGACTGCTAAGGAGACTGCTATTTTGTCCAAAGTGGTTAACCAAGAAGAATTGCTTATACAAATTACTGAGAAATGCCTCAAAATCTTTGATCACTCAAATAAAAAGGGTAGGAATATGAAGTGGCCTGCTACTGCTAGCAATGAAAAGGGGAAGTTGATGAGTACTGATTTGGGATTAGGGTTTGTGGATAAGAAATCAAGGGCAGACCATGATCAAGATCAAGACCATGATCTACAGGTGACGAAAACGTTTCGTCTAAAAAAACTTTACAGTACGAACAGGGCTGTCTCTGAGATTTTGGGGCCCTATGCGAGACTCCAAAGGAGGCTTTTAAAAAGTATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0042221 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071369 GO:0071495 GO:0080090 GO:0080167 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

375

Amino Acids

43.02

Weight (kDa)

7.67

Isoelectric Point (pI)

52.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	30 - 276	1.5e-104	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009800)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10120 AT5G65100
fragaria_vesca	FvH4_1g21280
malus_domestica	MD01G1025900.v1.1 MD15G1327300.v1.1
prunus_persica	Prupe.6G181600_v2.0.a1
pyrus_communis	pycom395g00020
rosa_chinensis	RchiOBHm_Chr2g0115111
rosa_laevigata	RLG00000018159
rosa_multiflora	Rmu_sc0002784.1_g000010
rosa_roxburghii	Rroxscaffold_2G00130590
rosa_rugosa	Rorug02G0193600 Rorug02G0193700
rosa_samantha	Rh2AG252000 Rh2AG252100 Rh2BG261600 Rh2BG261700 Rh2CG256600 Rh2CG256700 Rh2DG260200 Rh2DG260300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	202
AciI	CCGC	2 cut(s)	551, 559
AclI	AACGTT	1 cut(s)	1028
AclWI	GGATC	3 cut(s)	123, 240, 471
AcuI	CTGAAG	1 cut(s)	90
AfaI	GTAC	2 cut(s)	943, 1054
AfiI	CCNNNNNNNGG	4 cut(s)	332, 347, 597, 727
AflII	CTTAAG	1 cut(s)	364
AgsI	TTSAA	1 cut(s)	449
AluBI	AGCT	5 cut(s)	84, 94, 363, 437, 745
AluI	AGCT	5 cut(s)	84, 94, 363, 437, 745
Alw21I	GWGCWC	1 cut(s)	96
Alw26I	GTCTC	4 cut(s)	228, 785, 1072, 1087
AlwI	GGATC	3 cut(s)	123, 240, 471
AoxI	GGCC	4 cut(s)	599, 717, 902, 1082
ApaI	GGGCCC	1 cut(s)	1086
ApeKI	GCWGC	2 cut(s)	538, 698
ArsI	GACNNNNNNTTYG	2 cut(s)	784, 816
AspS9I	GGNCC	5 cut(s)	600, 628, 718, 1082, 1083
AsuHPI	GGTGA	3 cut(s)	286, 467, 1030
AsuNHI	GCTAGC	1 cut(s)	914
AvaII	GGWCC	1 cut(s)	628
BaeGI	GKGCMC	1 cut(s)	1086
BanII	GRGCYC	3 cut(s)	96, 353, 1086
BbsI	GAAGAC	1 cut(s)	177
Bbv12I	GWGCWC	1 cut(s)	96
BbvI	GCAGC	2 cut(s)	550, 685
BclI	TGATCA	3 cut(s)	705, 868, 991
BcoDI	GTCTC	4 cut(s)	228, 785, 1072, 1087
BfaI	CTAG	1 cut(s)	915
BfmI	CTRYAG	3 cut(s)	195, 642, 1013
BfrI	CTTAAG	1 cut(s)	364
BfuAI	ACCTGC	1 cut(s)	202
BisI	GCNGC	3 cut(s)	539, 551, 699
BlsI	GCNGC	3 cut(s)	540, 552, 700
BmcAI	AGTACT	1 cut(s)	943
Bme18I	GGWCC	1 cut(s)	628
BmgT120I	GGNCC	5 cut(s)	600, 628, 718, 1082, 1083
BmiI	GGNNCC	7 cut(s)	352, 566, 588, 629, 720, 1083, 1084
BmsI	GCATC	3 cut(s)	74, 436, 525
BmtI	GCTAGC	1 cut(s)	918
BpiI	GAAGAC	1 cut(s)	177
BplI	GAGNNNNNCTC	2 cut(s)	553, 585
BpmI	CTGGAG	1 cut(s)	593
Bpu10I	CCTNAGC	1 cut(s)	786
BpuEI	CTTGAG	1 cut(s)	80
BsaBI	GATNNNNATC	1 cut(s)	996
BsaJI	CCNNGG	5 cut(s)	13, 593, 603, 631, 693
BsaXI	ACNNNNNCTCC	4 cut(s)	602, 605, 632, 635
Bsc4I	CCNNNNNNNGG	4 cut(s)	332, 347, 597, 727
Bse3DI	GCAATG	2 cut(s)	411, 925
Bse8I	GATNNNNATC	1 cut(s)	996
BseDI	CCNNGG	5 cut(s)	13, 593, 603, 631, 693
BseJI	GATNNNNATC	1 cut(s)	996
BseLI	CCNNNNNNNGG	4 cut(s)	332, 347, 597, 727
BseMI	GCAATG	2 cut(s)	411, 925
BseMII	CTCAG	3 cut(s)	318, 837, 1062
BseRI	GAGGAG	3 cut(s)	343, 626, 648
BseSI	GKGCMC	1 cut(s)	1086
BseXI	GCAGC	2 cut(s)	550, 685
BshFI	GGCC	4 cut(s)	601, 719, 904, 1084
BsiHKAI	GWGCWC	1 cut(s)	96
BslFI	GGGAC	1 cut(s)	641
BslI	CCNNNNNNNGG	4 cut(s)	332, 347, 597, 727
BsmAI	GTCTC	4 cut(s)	228, 785, 1072, 1087
BsmBI	CGTCTC	1 cut(s)	228
BsmFI	GGGAC	1 cut(s)	641
BsnI	GGCC	4 cut(s)	601, 719, 904, 1084
Bsp120I	GGGCCC	1 cut(s)	1082
Bsp1286I	GDGCHC	3 cut(s)	96, 353, 1086
Bsp143I	GATC	9 cut(s)	73, 115, 245, 463, 705, 868, 991, 997, 1009
Bsp19I	CCATGG	1 cut(s)	13
BspACI	CCGC	2 cut(s)	551, 559
BspANI	GGCC	4 cut(s)	601, 719, 904, 1084
BspCNI	CTCAG	3 cut(s)	319, 838, 1063
BspHI	TCATGA	1 cut(s)	490
BspLI	GGNNCC	7 cut(s)	352, 566, 588, 629, 720, 1083, 1084
BspMAI	CTGCAG	1 cut(s)	199
BspMI	ACCTGC	1 cut(s)	202
BspOI	GCTAGC	1 cut(s)	918
BspPI	GGATC	3 cut(s)	123, 240, 471
BspQI	GCTCTTC	1 cut(s)	84
BspTI	CTTAAG	1 cut(s)	364
BsrDI	GCAATG	2 cut(s)	411, 925
BssECI	CCNNGG	5 cut(s)	13, 593, 603, 631, 693
BssMI	GATC	9 cut(s)	73, 115, 245, 463, 705, 868, 991, 997, 1009
BssT1I	CCWWGG	4 cut(s)	13, 593, 631, 693
Bst4CI	ACNGT	3 cut(s)	31, 176, 1052
Bst6I	CTCTTC	1 cut(s)	84
BstAFI	CTTAAG	1 cut(s)	364
BstAPI	GCANNNNNTGC	1 cut(s)	547
BstC8I	GCNNGC	4 cut(s)	435, 548, 906, 916
BstDEI	CTNAG	4 cut(s)	327, 786, 846, 1071
BstDSI	CCRYGG	2 cut(s)	13, 603
BstKTI	GATC	9 cut(s)	76, 118, 248, 466, 708, 871, 994, 1000, 1012
BstMAI	GTCTC	4 cut(s)	228, 785, 1072, 1087
BstMBI	GATC	9 cut(s)	73, 115, 245, 463, 705, 868, 991, 997, 1009
BstMWI	GCNNNNNNNGC	6 cut(s)	100, 413, 547, 556, 698, 751
BstNSI	RCATGY	2 cut(s)	550, 653
BstSFI	CTRYAG	3 cut(s)	195, 642, 1013
BstSLI	GKGCMC	1 cut(s)	1086
BstV1I	GCAGC	2 cut(s)	550, 685
BstV2I	GAAGAC	1 cut(s)	177
BstX2I	RGATCY	1 cut(s)	245
BstYI	RGATCY	1 cut(s)	245
BsuRI	GGCC	4 cut(s)	601, 719, 904, 1084
BtgI	CCRYGG	2 cut(s)	13, 603
BtsI	GCAGTG	1 cut(s)	541
BtsIMutI	CAGTG	1 cut(s)	541
BveI	ACCTGC	1 cut(s)	202
Cac8I	GCNNGC	4 cut(s)	435, 548, 906, 916
CciI	TCATGA	1 cut(s)	490
Cfr13I	GGNCC	5 cut(s)	600, 628, 718, 1082, 1083
CseI	GACGC	1 cut(s)	210
Csp6I	GTAC	2 cut(s)	942, 1053
CspCI	CAANNNNNGTGG	2 cut(s)	127, 162
CviQI	GTAC	2 cut(s)	942, 1053
DdeI	CTNAG	4 cut(s)	327, 786, 846, 1071
DpnI	GATC	9 cut(s)	75, 117, 247, 465, 707, 870, 993, 999, 1011
DpnII	GATC	9 cut(s)	73, 115, 245, 463, 705, 868, 991, 997, 1009
DraI	TTTAAA	1 cut(s)	1113
Eam1104I	CTCTTC	1 cut(s)	84
EarI	CTCTTC	1 cut(s)	84
Ecl136II	GAGCTC	1 cut(s)	94
Eco130I	CCWWGG	4 cut(s)	13, 593, 631, 693
Eco24I	GRGCYC	3 cut(s)	96, 353, 1086
Eco47I	GGWCC	1 cut(s)	628
Eco53kI	GAGCTC	1 cut(s)	94
Eco57I	CTGAAG	1 cut(s)	90
EcoICRI	GAGCTC	1 cut(s)	94
EcoO109I	RGGNCCY	2 cut(s)	1082, 1083
EcoT14I	CCWWGG	4 cut(s)	13, 593, 631, 693
EcoT38I	GRGCYC	3 cut(s)	96, 353, 1086
ErhI	CCWWGG	4 cut(s)	13, 593, 631, 693
Esp3I	CGTCTC	1 cut(s)	228
FalI	AAGNNNNNCTT	2 cut(s)	816, 848
FaqI	GGGAC	1 cut(s)	641
FbaI	TGATCA	3 cut(s)	705, 868, 991
Fnu4HI	GCNGC	3 cut(s)	539, 551, 699
FriOI	GRGCYC	3 cut(s)	96, 353, 1086
Fsp4HI	GCNGC	3 cut(s)	539, 551, 699
FspBI	CTAG	1 cut(s)	915
GluI	GCNGC	3 cut(s)	539, 551, 699
GsuI	CTGGAG	1 cut(s)	593
HaeIII	GGCC	4 cut(s)	601, 719, 904, 1084
HgaI	GACGC	1 cut(s)	210
HincII	GTYRAC	1 cut(s)	817
HindII	GTYRAC	1 cut(s)	817
HindIII	AAGCTT	1 cut(s)	743
HinfI	GANTC	3 cut(s)	200, 254, 1095
HpaI	GTTAAC	1 cut(s)	817
HphI	GGTGA	3 cut(s)	286, 467, 1030
Hpy166II	GTNNAC	1 cut(s)	817
Hpy188I	TCNGA	5 cut(s)	70, 424, 534, 730, 1072
Hpy188III	TCNNGA	5 cut(s)	97, 491, 572, 995, 1001
Hpy8I	GTNNAC	1 cut(s)	817
Hpy99I	CGWCG	1 cut(s)	231
HpyAV	CCTTC	3 cut(s)	106, 325, 571
HpyCH4III	ACNGT	3 cut(s)	31, 176, 1052
HpyCH4IV	ACGT	1 cut(s)	1028
HpyCH4V	TGCA	7 cut(s)	197, 294, 416, 499, 538, 546, 769
HpyF10VI	GCNNNNNNNGC	6 cut(s)	100, 413, 547, 556, 698, 751
HpyF3I	CTNAG	4 cut(s)	327, 786, 846, 1071
HpySE526I	ACGT	1 cut(s)	1028
Ksp22I	TGATCA	3 cut(s)	705, 868, 991
KspAI	GTTAAC	1 cut(s)	817
Kzo9I	GATC	9 cut(s)	73, 115, 245, 463, 705, 868, 991, 997, 1009
LguI	GCTCTTC	1 cut(s)	84
LmnI	GCTCC	3 cut(s)	356, 592, 661
LpnPI	CCDG	7 cut(s)	207, 339, 447, 557, 918, 1001, 1045
Lsp1109I	GCAGC	2 cut(s)	550, 685
LweI	GCATC	3 cut(s)	74, 436, 525
MaeI	CTAG	1 cut(s)	915
MaeII	ACGT	1 cut(s)	1028
MaeIII	GTNAC	1 cut(s)	1018
MalI	GATC	9 cut(s)	75, 117, 247, 465, 707, 870, 993, 999, 1011
MboI	GATC	9 cut(s)	73, 115, 245, 463, 705, 868, 991, 997, 1009
MflI	RGATCY	1 cut(s)	245
MhlI	GDGCHC	3 cut(s)	96, 353, 1086
MluCI	AATT	3 cut(s)	724, 827, 840
MlyI	GAGTC	2 cut(s)	209, 1089
MmeI	TCCRAC	1 cut(s)	441
MseI	TTAA	4 cut(s)	365, 669, 816, 1112
MspA1I	CMGCKG	1 cut(s)	559
MspCI	CTTAAG	1 cut(s)	364
MwoI	GCNNNNNNNGC	6 cut(s)	100, 413, 547, 556, 698, 751
NcoI	CCATGG	1 cut(s)	13
NdeII	GATC	9 cut(s)	73, 115, 245, 463, 705, 868, 991, 997, 1009
NheI	GCTAGC	1 cut(s)	914
NlaIV	GGNNCC	7 cut(s)	352, 566, 588, 629, 720, 1083, 1084
NmuCI	GTSAC	1 cut(s)	1018
NspI	RCATGY	2 cut(s)	550, 653
PaeI	GCATGC	1 cut(s)	550
PagI	TCATGA	1 cut(s)	490
PciSI	GCTCTTC	1 cut(s)	84
PfeI	GAWTC	1 cut(s)	254
PkrI	GCNGC	3 cut(s)	540, 552, 700
PleI	GAGTC	2 cut(s)	208, 1089
PpsI	GAGTC	2 cut(s)	208, 1089
Psp124BI	GAGCTC	1 cut(s)	96
Psp1406I	AACGTT	1 cut(s)	1028
PspN4I	GGNNCC	7 cut(s)	352, 566, 588, 629, 720, 1083, 1084
PspOMI	GGGCCC	1 cut(s)	1082
PspPI	GGNCC	5 cut(s)	600, 628, 718, 1082, 1083
PstI	CTGCAG	1 cut(s)	199
PsuI	RGATCY	1 cut(s)	245
RsaI	GTAC	2 cut(s)	943, 1054
RsaNI	GTAC	2 cut(s)	942, 1053
SacI	GAGCTC	1 cut(s)	96
SapI	GCTCTTC	1 cut(s)	84
SaqAI	TTAA	4 cut(s)	365, 669, 816, 1112
SatI	GCNGC	3 cut(s)	539, 551, 699
Sau3AI	GATC	9 cut(s)	73, 115, 245, 463, 705, 868, 991, 997, 1009
Sau96I	GGNCC	5 cut(s)	600, 628, 718, 1082, 1083
ScaI	AGTACT	1 cut(s)	943
SchI	GAGTC	2 cut(s)	209, 1089
SduI	GDGCHC	3 cut(s)	96, 353, 1086
SfaNI	GCATC	3 cut(s)	74, 436, 525
SfcI	CTRYAG	3 cut(s)	195, 642, 1013
SinI	GGWCC	1 cut(s)	628
SmlI	CTYRAG	2 cut(s)	95, 364
SmoI	CTYRAG	2 cut(s)	95, 364
SphI	GCATGC	1 cut(s)	550
Sse9I	AATT	3 cut(s)	724, 827, 840
SsiI	CCGC	2 cut(s)	551, 559
SspMI	CTAG	1 cut(s)	915
SstI	GAGCTC	1 cut(s)	96
StyI	CCWWGG	4 cut(s)	13, 593, 631, 693
TaaI	ACNGT	3 cut(s)	31, 176, 1052
TaiI	ACGT	1 cut(s)	1031
TaqI	TCGA	2 cut(s)	229, 257
TasI	AATT	3 cut(s)	724, 827, 840
TatI	WGTACW	1 cut(s)	941
TauI	GCSGC	1 cut(s)	553
TfiI	GAWTC	1 cut(s)	254
Tru1I	TTAA	4 cut(s)	365, 669, 816, 1112
Tru9I	TTAA	4 cut(s)	365, 669, 816, 1112
TscAI	CASTG	1 cut(s)	548
TseFI	GTSAC	1 cut(s)	1018
TseI	GCWGC	2 cut(s)	538, 698
Tsp45I	GTSAC	1 cut(s)	1018
TspDTI	ATGAA	5 cut(s)	198, 479, 507, 911, 936
TspRI	CASTG	1 cut(s)	548
Vha464I	CTTAAG	1 cut(s)	364
VpaK11BI	GGWCC	1 cut(s)	628
XceI	RCATGY	2 cut(s)	550, 653
XspI	CTAG	1 cut(s)	915
ZrmI	AGTACT	1 cut(s)	943

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.