RLG00000018159

ETHYLENE INSENSITIVE 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009800
Physical Location & Seq
Forward (+)
26225581 .. 26226911
1331 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018159

Sequence Viewer

Length: 1161 bp
ATGGTAGAGATCCTTGAAGAAGTTGATCCTCGAAGATTGGAAGATGAAGAAGCTGAAGATATCAGCTACGAGGATCTCAAGAAGCGAATAAGGAAGGATCGTGTGCGCTTAGAGAAGCTCAAGGAAATCCGAAAGAGCGAAGAACCCGAGTCGGCTAAAGAAGAGGCGTCTCGCCGAAAGAAGATGGCTAGAGCTCAAGATTCCATACTCAAATACATGGTGAAGATCATGGAAGTTTGCCAAGCACGAGGCTTCGTCTATGGGATCGTTCCGAAGAAGAGGAAGCTGGTGACAGGATCGTCGGATAGCTTACGAGAGTGGTGGAAAGACAAAGTCCGGTTCGACCAGAACGCTCCACTTGCAATTTCCAAGAATCTACCTGAAGTGATTTTGGAACAAGCAGGCACTAGTAGGCTAGATCATGACAACTCTTATGTCCATCTCCTTCATGACTTGCAAGACACGACGCTGGGGTCGCTGCTTTCAGCTCTAATGCAACACTGTAAGCCTCCACAGAGGCGGTTTCCGTTGGAGAAAGGTTTGGCTCCGCCGTGGTGGCCTAACGGAAATGAGATATGGTGGGGTGAACAAGGGGTTTCTCAAGAGCATGGGCCTCCTCCATATAGGAAACCTCATGATCTCAAAAAGGCTTGGAAAAAACCAATCAGCTGCCCCGAAATCGCGGGCACCACGGCCTTACATTCTAACGAAAAGAGGAAGTGCATGTTTGATGACGATTGCTCTGAATTGGATGCTCCACATGTTTTTCCAAAAACTTCAAACAGTGAATTAGGTTTAGGCTTTGTGGATAAGAAATCAAGGGCAGGCCATGATCAAAATAATGTTTCTGATTCAATGCGGGAGGTTGACACAAGTTCTTCGTATGATCATTCCTCAAATGATTCAGGATTCGAAAGTGTGGTGGAATGGATTAATATGGAGCTACAAAAGGCCAGCATAAACGAAGTCGGTGGAGTGGTCCTGTCTGGTTTTCCAAATACTTACTGGGAAGGTGGAATGGAAGAATTAGCTTTGGATGCAGCATTTGAGATTCAAAGGCAAAACATGGATTTGAATTTATGTCCAGAAGAAGGAATCTCACAGGTGAATCAAGACTCAACTTCAATTTGGGATATGGGATATGATTATGATAATTTATAA

Protein Analysis

387

Amino Acids

44.14

Weight (kDa)

5.22

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIN3_DNA-bd PF04873 24 - 220 1.3e-90 Ethylene insensitive 3, DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1159
AasI GACNNNNNNGTC 2 cut(s) 298, 472
AccB1I GGYRCC 1 cut(s) 686
AccII CGCG 1 cut(s) 683
AciI CCGC 4 cut(s) 520, 548, 683, 859
AclWI GGATC 6 cut(s) 4, 20, 81, 105, 272, 304
AcsI RAATTY 1 cut(s) 1075
AcuI CTGAAG 2 cut(s) 75, 402
AcyI GRCGYC 1 cut(s) 167
AfiI CCNNNNNNNGG 1 cut(s) 1091
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 6 cut(s) 17, 780, 855, 1055, 1075, 1125
AhlI ACTAGT 1 cut(s) 407
Alw21I GWGCWC 1 cut(s) 196
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 6 cut(s) 4, 20, 81, 105, 272, 304
Ama87I CYCGRG 1 cut(s) 146
AoxI GGCC 5 cut(s) 557, 611, 693, 826, 951
ApeKI GCWGC 3 cut(s) 478, 669, 1040
ApoI RAATTY 1 cut(s) 1075
AseI ATTAAT 1 cut(s) 933
AspLEI GCGC 1 cut(s) 108
AspS9I GGNCC 2 cut(s) 611, 979
AsuHPI GGTGA 4 cut(s) 232, 301, 596, 1117
AsuII TTCGAA 1 cut(s) 912
AvaI CYCGRG 1 cut(s) 146
AvaII GGWCC 1 cut(s) 979
BaeGI GKGCMC 1 cut(s) 689
BanI GGYRCC 1 cut(s) 686
BanII GRGCYC 1 cut(s) 196
BauI CACGAG 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 196
BbvI GCAGC 3 cut(s) 465, 656, 1052
BccI CCATC 2 cut(s) 178, 447
BceAI ACGGC 2 cut(s) 535, 708
BclI TGATCA 2 cut(s) 832, 886
BcoDI GTCTC 1 cut(s) 174
BcuI ACTAGT 1 cut(s) 407
BfaI CTAG 3 cut(s) 189, 408, 416
BglI GCCNNNNNGGC 1 cut(s) 556
BisI GCNGC 3 cut(s) 479, 670, 1041
BlsI GCNGC 3 cut(s) 480, 671, 1042
Bme18I GGWCC 1 cut(s) 979
BmeT110I CYCGRG 1 cut(s) 146
BmgT120I GGNCC 2 cut(s) 611, 979
BmiI GGNNCC 2 cut(s) 546, 688
BmrI ACTGGG 1 cut(s) 1015
BmsI GCATC 2 cut(s) 742, 1027
BmuI ACTGGG 1 cut(s) 1015
Bpu14I TTCGAA 1 cut(s) 912
BpuEI CTTGAG 4 cut(s) 62, 104, 180, 585
BsaHI GRCGYC 1 cut(s) 167
BsaJI CCNNGG 2 cut(s) 551, 690
BsaWI WCCGGW 1 cut(s) 336
Bsc4I CCNNNNNNNGG 1 cut(s) 1091
Bse1I ACTGG 1 cut(s) 1010
BseDI CCNNGG 2 cut(s) 551, 690
BseGI GGATG 2 cut(s) 757, 1042
BseLI CCNNNNNNNGG 1 cut(s) 1091
BseNI ACTGG 1 cut(s) 1010
BseRI GAGGAG 1 cut(s) 606
BseSI GKGCMC 1 cut(s) 689
BseXI GCAGC 3 cut(s) 465, 656, 1052
BseYI CCCAGC 1 cut(s) 469
Bsh1236I CGCG 1 cut(s) 683
BshFI GGCC 5 cut(s) 559, 613, 695, 828, 953
BshNI GGYRCC 1 cut(s) 686
BsiHKAI GWGCWC 1 cut(s) 196
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 1 cut(s) 337
BslI CCNNNNNNNGG 1 cut(s) 1091
BsmAI GTCTC 1 cut(s) 174
BsmBI CGTCTC 1 cut(s) 174
BsnI GGCC 5 cut(s) 559, 613, 695, 828, 953
BsoBI CYCGRG 1 cut(s) 146
Bsp119I TTCGAA 1 cut(s) 912
Bsp1286I GDGCHC 2 cut(s) 196, 689
BspACI CCGC 4 cut(s) 520, 548, 683, 859
BspANI GGCC 5 cut(s) 559, 613, 695, 828, 953
BspFNI CGCG 1 cut(s) 683
BspHI TCATGA 3 cut(s) 421, 448, 634
BspLI GGNNCC 2 cut(s) 546, 688
BspPI GGATC 6 cut(s) 4, 20, 81, 105, 272, 304
BspT104I TTCGAA 1 cut(s) 912
BspT107I GGYRCC 1 cut(s) 686
BsrI ACTGG 1 cut(s) 1010
BssECI CCNNGG 2 cut(s) 551, 690
BssNI GRCGYC 1 cut(s) 167
BssSI CACGAG 1 cut(s) 246
Bst2BI CACGAG 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 503, 785
Bst6I CTCTTC 2 cut(s) 156, 272
BstACI GRCGYC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 912
BstC8I GCNNGC 4 cut(s) 403, 685, 826, 955
BstDEI CTNAG 1 cut(s) 109
BstDSI CCRYGG 2 cut(s) 551, 690
BstF5I GGATG 2 cut(s) 757, 1042
BstFNI CGCG 1 cut(s) 683
BstHHI GCGC 1 cut(s) 108
BstMAI GTCTC 1 cut(s) 174
BstMWI GCNNNNNNNGC 4 cut(s) 359, 475, 556, 1037
BstNSI RCATGY 2 cut(s) 727, 764
BstSLI GKGCMC 1 cut(s) 689
BstUI CGCG 1 cut(s) 683
BstV1I GCAGC 3 cut(s) 465, 656, 1052
BstX2I RGATCY 2 cut(s) 9, 73
BstYI RGATCY 2 cut(s) 9, 73
BsuRI GGCC 5 cut(s) 559, 613, 695, 828, 953
BtgI CCRYGG 2 cut(s) 551, 690
BtsCI GGATG 2 cut(s) 757, 1042
BtsIMutI CAGTG 2 cut(s) 499, 790
Cac8I GCNNGC 4 cut(s) 403, 685, 826, 955
CciI TCATGA 3 cut(s) 421, 448, 634
CfoI GCGC 1 cut(s) 108
Cfr13I GGNCC 2 cut(s) 611, 979
CseI GACGC 2 cut(s) 156, 475
DdeI CTNAG 1 cut(s) 109
DrdI GACNNNNNNGTC 2 cut(s) 298, 472
DseDI GACNNNNNNGTC 2 cut(s) 298, 472
Eam1104I CTCTTC 2 cut(s) 156, 272
EarI CTCTTC 2 cut(s) 156, 272
EciI GGCGGA 1 cut(s) 537
Ecl136II GAGCTC 1 cut(s) 194
Eco24I GRGCYC 1 cut(s) 196
Eco32I GATATC 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 979
Eco53kI GAGCTC 1 cut(s) 194
Eco57I CTGAAG 2 cut(s) 75, 402
Eco88I CYCGRG 1 cut(s) 146
EcoICRI GAGCTC 1 cut(s) 194
EcoRV GATATC 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 174
FauI CCCGC 2 cut(s) 676, 852
FbaI TGATCA 2 cut(s) 832, 886
Fnu4HI GCNGC 3 cut(s) 479, 670, 1041
FokI GGATG 2 cut(s) 764, 1049
FriOI GRGCYC 1 cut(s) 196
Fsp4HI GCNGC 3 cut(s) 479, 670, 1041
FspBI CTAG 3 cut(s) 189, 408, 416
GlaI GCGC 1 cut(s) 107
GluI GCNGC 3 cut(s) 479, 670, 1041
GsaI CCCAGC 1 cut(s) 473
HaeIII GGCC 5 cut(s) 559, 613, 695, 828, 953
HapII CCGG 1 cut(s) 337
HgaI GACGC 2 cut(s) 156, 475
HhaI GCGC 1 cut(s) 108
Hin1I GRCGYC 1 cut(s) 167
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HincII GTYRAC 1 cut(s) 868
HindII GTYRAC 1 cut(s) 868
HpaII CCGG 1 cut(s) 337
HphI GGTGA 4 cut(s) 232, 301, 596, 1117
Hpy166II GTNNAC 2 cut(s) 587, 868
Hpy188I TCNGA 5 cut(s) 131, 273, 304, 745, 850
Hpy188III TCNNGA 9 cut(s) 79, 197, 422, 449, 602, 635, 906, 1085, 1112
Hpy8I GTNNAC 2 cut(s) 587, 868
Hpy99I CGWCG 2 cut(s) 304, 469
HpyAV CCTTC 4 cut(s) 88, 455, 1004, 1085
HpyCH4III ACNGT 2 cut(s) 503, 785
HpyCH4V TGCA 5 cut(s) 362, 457, 496, 723, 1040
HpyF10VI GCNNNNNNNGC 4 cut(s) 359, 475, 556, 1037
HpyF3I CTNAG 1 cut(s) 109
Hsp92I GRCGYC 1 cut(s) 167
HspAI GCGC 1 cut(s) 106
Ksp22I TGATCA 2 cut(s) 832, 886
LmnI GCTCC 4 cut(s) 358, 550, 760, 940
Lsp1109I GCAGC 3 cut(s) 465, 656, 1052
LweI GCATC 2 cut(s) 742, 1027
MaeI CTAG 3 cut(s) 189, 408, 416
MaeIII GTNAC 1 cut(s) 289
MflI RGATCY 2 cut(s) 9, 73
MhlI GDGCHC 2 cut(s) 196, 689
MluCI AATT 7 cut(s) 363, 746, 788, 1025, 1075, 1125, 1153
MlyI GAGTC 2 cut(s) 158, 1109
MmeI TCCRAC 2 cut(s) 282, 510
MseI TTAA 1 cut(s) 933
MspA1I CMGCKG 1 cut(s) 669
MspI CCGG 1 cut(s) 337
MvnI CGCG 1 cut(s) 683
MwoI GCNNNNNNNGC 4 cut(s) 359, 475, 556, 1037
NlaIV GGNNCC 2 cut(s) 546, 688
NmuCI GTSAC 1 cut(s) 289
NspI RCATGY 2 cut(s) 727, 764
NspV TTCGAA 1 cut(s) 912
PagI TCATGA 3 cut(s) 421, 448, 634
PciI ACATGT 1 cut(s) 760
PfeI GAWTC 8 cut(s) 200, 373, 851, 902, 909, 1051, 1095, 1108
PflFI GACNNNGTC 1 cut(s) 332
PkrI GCNGC 3 cut(s) 480, 671, 1042
PleI GAGTC 2 cut(s) 157, 1109
PpsI GAGTC 2 cut(s) 157, 1109
PscI ACATGT 1 cut(s) 760
PshBI ATTAAT 1 cut(s) 933
PsiI TTATAA 1 cut(s) 1159
Psp124BI GAGCTC 1 cut(s) 196
PspFI CCCAGC 1 cut(s) 469
PspN4I GGNNCC 2 cut(s) 546, 688
PspPI GGNCC 2 cut(s) 611, 979
PsuI RGATCY 2 cut(s) 9, 73
PsyI GACNNNGTC 1 cut(s) 332
PvuII CAGCTG 1 cut(s) 669
SacI GAGCTC 1 cut(s) 196
SaqAI TTAA 1 cut(s) 933
SatI GCNGC 3 cut(s) 479, 670, 1041
Sau96I GGNCC 2 cut(s) 611, 979
SchI GAGTC 2 cut(s) 158, 1109
SduI GDGCHC 2 cut(s) 196, 689
SfaNI GCATC 2 cut(s) 742, 1027
SfuI TTCGAA 1 cut(s) 912
SinI GGWCC 1 cut(s) 979
SmlI CTYRAG 4 cut(s) 77, 119, 195, 600
SmoI CTYRAG 4 cut(s) 77, 119, 195, 600
SpeI ACTAGT 1 cut(s) 407
Sse9I AATT 7 cut(s) 363, 746, 788, 1025, 1075, 1125, 1153
SsiI CCGC 4 cut(s) 520, 548, 683, 859
SspMI CTAG 3 cut(s) 189, 408, 416
SstI GAGCTC 1 cut(s) 196
TaaI ACNGT 2 cut(s) 503, 785
TaqI TCGA 3 cut(s) 31, 342, 912
TasI AATT 7 cut(s) 363, 746, 788, 1025, 1075, 1125, 1153
TfiI GAWTC 8 cut(s) 200, 373, 851, 902, 909, 1051, 1095, 1108
Tru1I TTAA 1 cut(s) 933
Tru9I TTAA 1 cut(s) 933
TscAI CASTG 2 cut(s) 506, 790
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 3 cut(s) 478, 669, 1040
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 2 cut(s) 60, 437
TspGWI ACGGA 2 cut(s) 516, 579
TspRI CASTG 2 cut(s) 506, 790
Tth111I GACNNNGTC 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 979
VspI ATTAAT 1 cut(s) 933
XapI RAATTY 1 cut(s) 1075
XceI RCATGY 2 cut(s) 727, 764
XcmI CCANNNNNNNNNTGG 1 cut(s) 1002
XspI CTAG 3 cut(s) 189, 408, 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.