Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0009800

Physical Location & Seq

Genomic Coordinates Reverse (-)

9825648 .. 9826952

Gene Structure 1305 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1025900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1305 bp

ATGGTAAAGTTCCATGGACAAGTTGATCCTCTAAGCCTTGGAGTAGTTGCAGAAGATGAAAAAGCAGCTGAAGATCACATCAGCTACGACGAGCTCAAGAAGCGAACGTGGAAGGATAGTATTCGCTTACAAAAGCTCAACGAGAATCGTAAAATAATTGAGGTAGAAGAAGACGAAGAAGAAGAACTTGCAGAGTCGTCGGCAAACCAAGAAGGGTCTCATCGAAAGAAAATGGCAAGAGCCCAGGATTCGATACTTAAATACATGGTGAAAATCATGGAGGTATGCAAAGCAAAGGGCTTTGTGTATGGGATTGTGCCTGAGAAGGGAAAGCCAGTGACGGGCTCCTCTGATAGCTTAAGAGAGTGGTGGAAAGACAAAGCGAGATTTGACCACAACGCCCCCATTGCAATCGCCAAGTACCTGCCAGCTCTGATATTTGAAGGTGAGTCGGATCATGGCTCTTATGCTCATTTGCTTCATGAACTGCAAGACACTACTTTAGGGTCTCTTCTTTCTGCTCTGATTCAGCACTGCGTGCCGCCGCAGCGGAGGTTCCCTCTGGAGAAGGGTTTAGCTCCTCCGTGGTGGCCCACAGGGAAGGAGATGTGGTGGGGGGACCAAGGGCTCTCTGTAGAGCACGGCGCTCCTCCTTATCGGAAGCCCCACGATCTTAAAAAGGCATGGAAAGTGAGTGCCTTGGCTGCTGTGGTCAAACACATGGCTCCTAATTCGGATAGAGTGAGAAGACTTGTGACGCAAAGCAAGTGTTTGCAAGGTAAGATGACTGCTAAGGAGACTGCTATTTGGTCCAAAGTGGTTAACCAAGAAGAATCTCTCATACAGCTTACAGAGAAATGCCTCAAAATCTCAGATCACTCAAACAAAAGGGGTAAGAATTTGAAGTGGCCTGCTACTACTAGCAATGAAAAGAGGAAGATGATAAGTATTGATTTGGGATTAGGGTTTGTGGAAAAGAAATCAAGGGCAGACTATGATCAAGGTCAAGATCAAGATCGAACACTTTGTGCTTCTGATCAAAATAGTGGTGCTGTTAGTGTGGTGGATTGGATTAATATGGAGATAGAAAAGGCCAATCGGATTGATGACAACAGTGGTACTAAAGTGGCAGACGGCTATGGATCAGCAAGCTATTGGGGAGGTGGTATTGATGATGATGATCTAGCTATGGATCGAGCACTGGAGATCCAAAGGGGAAACATGGATTTAAATCGTTCTTCCGAAGATATGTCACATAATTATGACCAAGACTCAACTTCTATTTGGGATTTGGGATATGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0042221 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071369 GO:0071495 GO:0080090 GO:0080167 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

435

Amino Acids

48.98

Weight (kDa)

5.87

Isoelectric Point (pI)

42.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	30 - 278	1e-115	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009800)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10120 AT5G65100
fragaria_vesca	FvH4_1g21280
malus_domestica	MD01G1025900.v1.1 MD15G1327300.v1.1
prunus_persica	Prupe.6G181600_v2.0.a1
pyrus_communis	pycom395g00020
rosa_chinensis	RchiOBHm_Chr2g0115111
rosa_laevigata	RLG00000018159
rosa_multiflora	Rmu_sc0002784.1_g000010
rosa_roxburghii	Rroxscaffold_2G00130590
rosa_rugosa	Rorug02G0193600 Rorug02G0193700
rosa_samantha	Rh2AG252000 Rh2AG252100 Rh2BG261600 Rh2BG261700 Rh2CG256600 Rh2CG256700 Rh2DG260200 Rh2DG260300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	432
AciI	CCGC	3 cut(s)	542, 545, 550
AclWI	GGATC	5 cut(s)	20, 462, 1150, 1200, 1201
AcsI	RAATTY	1 cut(s)	898
AcuI	CTGAAG	1 cut(s)	90
AdeI	CACNNNGTG	2 cut(s)	538, 1028
AfaI	GTAC	2 cut(s)	422, 1120
AfiI	CCNNNNNNNGG	2 cut(s)	326, 341
AflII	CTTAAG	1 cut(s)	358
AgsI	TTSAA	2 cut(s)	443, 904
AjnI	CCWGG	1 cut(s)	243
Alw21I	GWGCWC	3 cut(s)	96, 642, 1201
Alw26I	GTCTC	3 cut(s)	222, 513, 791
AlwI	GGATC	5 cut(s)	20, 462, 1150, 1200, 1201
AoxI	GGCC	3 cut(s)	590, 908, 1092
ApeKI	GCWGC	3 cut(s)	65, 547, 704
ApoI	RAATTY	1 cut(s)	898
AseI	ATTAAT	1 cut(s)	1074
AspLEI	GCGC	1 cut(s)	647
AspS9I	GGNCC	3 cut(s)	591, 619, 810
AsuHPI	GGTGA	2 cut(s)	280, 458
AvaII	GGWCC	2 cut(s)	619, 810
BaeI	ACNNNNGTAYC	2 cut(s)	1110, 1143
BanII	GRGCYC	4 cut(s)	96, 244, 347, 630
BbsI	GAAGAC	2 cut(s)	177, 754
Bbv12I	GWGCWC	3 cut(s)	96, 642, 1201
BbvI	GCAGC	3 cut(s)	77, 559, 691
BceAI	ACGGC	2 cut(s)	658, 1150
BcgI	CGANNNNNNTGC	2 cut(s)	180, 214
BciT130I	CCWGG	1 cut(s)	245
BclI	TGATCA	2 cut(s)	997, 1036
BcoDI	GTCTC	3 cut(s)	222, 513, 791
BfaI	CTAG	2 cut(s)	921, 1184
BfmI	CTRYAG	1 cut(s)	633
BfoI	RGCGCY	1 cut(s)	648
BfrI	CTTAAG	1 cut(s)	358
BfuAI	ACCTGC	1 cut(s)	432
BisI	GCNGC	5 cut(s)	66, 542, 545, 548, 705
BlsI	GCNGC	5 cut(s)	67, 543, 546, 549, 706
Bme1390I	CCNGG	1 cut(s)	245
Bme18I	GGWCC	2 cut(s)	619, 810
BmgT120I	GGNCC	3 cut(s)	591, 619, 810
BmiI	GGNNCC	4 cut(s)	346, 557, 620, 726
BmrFI	CCNGG	1 cut(s)	245
BpiI	GAAGAC	2 cut(s)	177, 754
BplI	GAGNNNNNCTC	2 cut(s)	544, 576
BpmI	CTGGAG	2 cut(s)	584, 1223
Bpu10I	CCTNAGC	1 cut(s)	792
BpuEI	CTTGAG	1 cut(s)	80
BsaBI	GATNNNNATC	3 cut(s)	1014, 1179, 1230
BsaI	GGTCTC	2 cut(s)	222, 513
BsaJI	CCNNGG	6 cut(s)	13, 37, 243, 584, 622, 699
BsaXI	ACNNNNNCTCC	2 cut(s)	596, 626
Bsc4I	CCNNNNNNNGG	2 cut(s)	326, 341
Bse1I	ACTGG	2 cut(s)	335, 1206
Bse3DI	GCAATG	2 cut(s)	405, 931
Bse8I	GATNNNNATC	3 cut(s)	1014, 1179, 1230
BseBI	CCWGG	1 cut(s)	245
BseDI	CCNNGG	6 cut(s)	13, 37, 243, 584, 622, 699
BseJI	GATNNNNATC	3 cut(s)	1014, 1179, 1230
BseLI	CCNNNNNNNGG	2 cut(s)	326, 341
BseMI	GCAATG	2 cut(s)	405, 931
BseMII	CTCAG	2 cut(s)	312, 885
BseNI	ACTGG	2 cut(s)	335, 1206
BseRI	GAGGAG	3 cut(s)	337, 570, 639
BseXI	GCAGC	3 cut(s)	77, 559, 691
BshFI	GGCC	3 cut(s)	592, 910, 1094
BsiHKAI	GWGCWC	3 cut(s)	96, 642, 1201
BslFI	GGGAC	1 cut(s)	632
BslI	CCNNNNNNNGG	2 cut(s)	326, 341
BsmAI	GTCTC	3 cut(s)	222, 513, 791
BsmFI	GGGAC	1 cut(s)	632
BsnI	GGCC	3 cut(s)	592, 910, 1094
Bso31I	GGTCTC	2 cut(s)	222, 513
Bsp1286I	GDGCHC	6 cut(s)	96, 244, 347, 630, 642, 1201
Bsp19I	CCATGG	1 cut(s)	13
BspACI	CCGC	3 cut(s)	542, 545, 550
BspANI	GGCC	3 cut(s)	592, 910, 1094
BspCNI	CTCAG	2 cut(s)	313, 884
BspHI	TCATGA	1 cut(s)	481
BspLI	GGNNCC	4 cut(s)	346, 557, 620, 726
BspMI	ACCTGC	1 cut(s)	432
BspPI	GGATC	5 cut(s)	20, 462, 1150, 1200, 1201
BspTI	CTTAAG	1 cut(s)	358
BspTNI	GGTCTC	2 cut(s)	222, 513
BsrDI	GCAATG	2 cut(s)	405, 931
BsrI	ACTGG	2 cut(s)	335, 1206
BssECI	CCNNGG	6 cut(s)	13, 37, 243, 584, 622, 699
BssT1I	CCWWGG	4 cut(s)	13, 37, 622, 699
Bst2UI	CCWGG	1 cut(s)	245
Bst4CI	ACNGT	1 cut(s)	1115
Bst6I	CTCTTC	1 cut(s)	516
BstAFI	CTTAAG	1 cut(s)	358
BstAPI	GCANNNNNTGC	1 cut(s)	538
BstC8I	GCNNGC	4 cut(s)	429, 539, 912, 1150
BstDEI	CTNAG	4 cut(s)	32, 321, 792, 871
BstDSI	CCRYGG	2 cut(s)	13, 584
BstH2I	RGCGCY	1 cut(s)	648
BstHHI	GCGC	1 cut(s)	647
BstMAI	GTCTC	3 cut(s)	222, 513, 791
BstMWI	GCNNNNNNNGC	5 cut(s)	100, 407, 538, 547, 704
BstNI	CCWGG	1 cut(s)	245
BstSCI	CCNGG	1 cut(s)	243
BstSFI	CTRYAG	1 cut(s)	633
BstV1I	GCAGC	3 cut(s)	77, 559, 691
BstV2I	GAAGAC	2 cut(s)	177, 754
BstX2I	RGATCY	1 cut(s)	1206
BstYI	RGATCY	1 cut(s)	1206
BsuRI	GGCC	3 cut(s)	592, 910, 1094
BtgI	CCRYGG	2 cut(s)	13, 584
BtsI	GCAGTG	1 cut(s)	532
BtsIMutI	CAGTG	4 cut(s)	342, 532, 1120, 1199
BveI	ACCTGC	1 cut(s)	432
Cac8I	GCNNGC	4 cut(s)	429, 539, 912, 1150
CciI	TCATGA	1 cut(s)	481
CfoI	GCGC	1 cut(s)	647
Cfr13I	GGNCC	3 cut(s)	591, 619, 810
CseI	GACGC	1 cut(s)	766
Csp6I	GTAC	2 cut(s)	421, 1119
CviAII	CATG	8 cut(s)	14, 265, 277, 458, 482, 684, 721, 1222
CviQI	GTAC	2 cut(s)	421, 1119
DdeI	CTNAG	4 cut(s)	32, 321, 792, 871
DraI	TTTAAA	1 cut(s)	1230
DraIII	CACNNNGTG	2 cut(s)	538, 1028
Eam1104I	CTCTTC	1 cut(s)	516
EarI	CTCTTC	1 cut(s)	516
Ecl136II	GAGCTC	1 cut(s)	94
Eco130I	CCWWGG	4 cut(s)	13, 37, 622, 699
Eco24I	GRGCYC	4 cut(s)	96, 244, 347, 630
Eco31I	GGTCTC	2 cut(s)	222, 513
Eco47I	GGWCC	2 cut(s)	619, 810
Eco53kI	GAGCTC	1 cut(s)	94
Eco57I	CTGAAG	1 cut(s)	90
EcoICRI	GAGCTC	1 cut(s)	94
EcoRII	CCWGG	1 cut(s)	243
EcoT14I	CCWWGG	4 cut(s)	13, 37, 622, 699
EcoT38I	GRGCYC	4 cut(s)	96, 244, 347, 630
ErhI	CCWWGG	4 cut(s)	13, 37, 622, 699
FaeI	CATG	8 cut(s)	17, 268, 280, 461, 485, 687, 724, 1225
FalI	AAGNNNNNCTT	2 cut(s)	171, 203
FaqI	GGGAC	1 cut(s)	632
FatI	CATG	8 cut(s)	13, 264, 276, 457, 481, 683, 720, 1221
FbaI	TGATCA	2 cut(s)	997, 1036
Fnu4HI	GCNGC	5 cut(s)	66, 542, 545, 548, 705
FriOI	GRGCYC	4 cut(s)	96, 244, 347, 630
Fsp4HI	GCNGC	5 cut(s)	66, 542, 545, 548, 705
FspBI	CTAG	2 cut(s)	921, 1184
GlaI	GCGC	1 cut(s)	646
GluI	GCNGC	5 cut(s)	66, 542, 545, 548, 705
GsuI	CTGGAG	2 cut(s)	584, 1223
HaeII	RGCGCY	1 cut(s)	648
HaeIII	GGCC	3 cut(s)	592, 910, 1094
HgaI	GACGC	1 cut(s)	766
HhaI	GCGC	1 cut(s)	647
Hin1II	CATG	8 cut(s)	17, 268, 280, 461, 485, 687, 724, 1225
Hin6I	GCGC	1 cut(s)	645
HinP1I	GCGC	1 cut(s)	645
HincII	GTYRAC	1 cut(s)	823
HindII	GTYRAC	1 cut(s)	823
HinfI	GANTC	7 cut(s)	145, 194, 248, 449, 526, 833, 1271
HpaI	GTTAAC	1 cut(s)	823
HphI	GGTGA	2 cut(s)	280, 458
Hpy166II	GTNNAC	1 cut(s)	823
Hpy188III	TCNNGA	5 cut(s)	97, 482, 563, 1007, 1013
Hpy8I	GTNNAC	1 cut(s)	823
Hpy99I	CGWCG	2 cut(s)	92, 202
HpyAV	CCTTC	6 cut(s)	106, 206, 319, 437, 562, 595
HpyCH4III	ACNGT	1 cut(s)	1115
HpyCH4IV	ACGT	1 cut(s)	107
HpyCH4V	TGCA	6 cut(s)	50, 191, 288, 410, 490, 775
HpyF10VI	GCNNNNNNNGC	5 cut(s)	100, 407, 538, 547, 704
HpyF3I	CTNAG	4 cut(s)	32, 321, 792, 871
HpySE526I	ACGT	1 cut(s)	107
Hsp92II	CATG	8 cut(s)	17, 268, 280, 461, 485, 687, 724, 1225
HspAI	GCGC	1 cut(s)	645
Ksp22I	TGATCA	2 cut(s)	997, 1036
KspAI	GTTAAC	1 cut(s)	823
LmnI	GCTCC	4 cut(s)	350, 583, 652, 730
Lsp1109I	GCAGC	3 cut(s)	77, 559, 691
MaeI	CTAG	2 cut(s)	921, 1184
MaeII	ACGT	1 cut(s)	107
MaeIII	GTNAC	3 cut(s)	337, 754, 1251
MflI	RGATCY	1 cut(s)	1206
MhlI	GDGCHC	6 cut(s)	96, 244, 347, 630, 642, 1201
MluCI	AATT	4 cut(s)	156, 730, 898, 1258
MlyI	GAGTC	3 cut(s)	203, 458, 1265
MmeI	TCCRAC	1 cut(s)	432
MseI	TTAA	7 cut(s)	258, 359, 675, 822, 1074, 1229, 1303
MslI	CAYNNNNRTG	1 cut(s)	1260
MspA1I	CMGCKG	2 cut(s)	68, 550
MspCI	CTTAAG	1 cut(s)	358
MspR9I	CCNGG	1 cut(s)	245
MvaI	CCWGG	1 cut(s)	245
MwoI	GCNNNNNNNGC	5 cut(s)	100, 407, 538, 547, 704
NcoI	CCATGG	1 cut(s)	13
NlaIII	CATG	8 cut(s)	17, 268, 280, 461, 485, 687, 724, 1225
NlaIV	GGNNCC	4 cut(s)	346, 557, 620, 726
NmuCI	GTSAC	3 cut(s)	337, 754, 1251
PagI	TCATGA	1 cut(s)	481
PfeI	GAWTC	4 cut(s)	145, 248, 526, 833
PkrI	GCNGC	5 cut(s)	67, 543, 546, 549, 706
PleI	GAGTC	3 cut(s)	202, 457, 1265
PpsI	GAGTC	3 cut(s)	202, 457, 1265
PshBI	ATTAAT	1 cut(s)	1074
Psp124BI	GAGCTC	1 cut(s)	96
Psp6I	CCWGG	1 cut(s)	243
PspGI	CCWGG	1 cut(s)	243
PspN4I	GGNNCC	4 cut(s)	346, 557, 620, 726
PspPI	GGNCC	3 cut(s)	591, 619, 810
PsuI	RGATCY	1 cut(s)	1206
PvuII	CAGCTG	1 cut(s)	68
RsaI	GTAC	2 cut(s)	422, 1120
RsaNI	GTAC	2 cut(s)	421, 1119
RseI	CAYNNNNRTG	1 cut(s)	1260
SacI	GAGCTC	1 cut(s)	96
SaqAI	TTAA	7 cut(s)	258, 359, 675, 822, 1074, 1229, 1303
SatI	GCNGC	5 cut(s)	66, 542, 545, 548, 705
Sau96I	GGNCC	3 cut(s)	591, 619, 810
SchI	GAGTC	3 cut(s)	203, 458, 1265
ScrFI	CCNGG	1 cut(s)	245
SduI	GDGCHC	6 cut(s)	96, 244, 347, 630, 642, 1201
SfcI	CTRYAG	1 cut(s)	633
SinI	GGWCC	2 cut(s)	619, 810
SmiI	ATTTAAAT	1 cut(s)	1230
SmiMI	CAYNNNNRTG	1 cut(s)	1260
SmlI	CTYRAG	2 cut(s)	95, 358
SmoI	CTYRAG	2 cut(s)	95, 358
Sse9I	AATT	4 cut(s)	156, 730, 898, 1258
SsiI	CCGC	3 cut(s)	542, 545, 550
SspMI	CTAG	2 cut(s)	921, 1184
SstI	GAGCTC	1 cut(s)	96
StyD4I	CCNGG	1 cut(s)	243
StyI	CCWWGG	4 cut(s)	13, 37, 622, 699
SwaI	ATTTAAAT	1 cut(s)	1230
TaaI	ACNGT	1 cut(s)	1115
TaiI	ACGT	1 cut(s)	110
TaqI	TCGA	4 cut(s)	223, 251, 1018, 1195
TasI	AATT	4 cut(s)	156, 730, 898, 1258
TauI	GCSGC	2 cut(s)	544, 547
TfiI	GAWTC	4 cut(s)	145, 248, 526, 833
Tru1I	TTAA	7 cut(s)	258, 359, 675, 822, 1074, 1229, 1303
Tru9I	TTAA	7 cut(s)	258, 359, 675, 822, 1074, 1229, 1303
TscAI	CASTG	4 cut(s)	342, 539, 1120, 1206
TseFI	GTSAC	3 cut(s)	337, 754, 1251
TseI	GCWGC	3 cut(s)	65, 547, 704
Tsp45I	GTSAC	3 cut(s)	337, 754, 1251
TspDTI	ATGAA	4 cut(s)	72, 470, 498, 942
TspGWI	ACGGA	1 cut(s)	573
TspRI	CASTG	4 cut(s)	342, 539, 1120, 1206
Vha464I	CTTAAG	1 cut(s)	358
VpaK11BI	GGWCC	2 cut(s)	619, 810
VspI	ATTAAT	1 cut(s)	1074
XapI	RAATTY	1 cut(s)	898
XspI	CTAG	2 cut(s)	921, 1184

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.